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1.
In 5 experiments with 1,296 laying hybrids at the age of 22-73 weeks producing eggs for consumption a varied supplementation of between 0 and 20 mg vitamin E per kg of a semisynthetic ration was tested. The vitamin E content of the basal ration was less than 1 mg per kg feed. The quotas of selenium and unsaturated fatty acids in the feed were taken into consideration. Vitamin E and Se supplements did not influence, feed intake, laying, performance, feed efficiency/100 g egg development of body weight and mortality. The supplementation of 14% vitamin-E-free sunflower oil caused a significant decrease of the laying performance by 15%. There was no relation between the humoral immune reaction and vitamin E supply. The peroxide value of the abdominal fat and the activities of the creatine phosphokinase, the aspartate and alanine amino transferase in the blood serum were not directionally changed by the vitamin E, Se and oil supplements. There were no significant relations between the vitamin E content of the feed and that of the yolk and the yolk sac of the chicken. A vitamin E requirement of less than 1 mg and a requirement norm, including a safety margin, of 5 mg per kg feed was derived from the results for laying hens for egg production. A supplement of synthetic vitamin E to laying hen feed is thus not necessary.  相似文献   

2.
In 4 experiments young chickens of the species White Leghorn received semisynthetic rations with a vitamin E content less than 1 mg/kg basal ration. Sunflower oil heated with FeCl3 supplement was used as a factor increasing requirement. Feed intake, live weight gain and feed expenditure with semisynthetic rations without oil supplements did not depend on vitamin E supplements. After the feeding of rations without vitamin E animal losses were higher. The losses were normalized by 1 mg vitamin E supplement per kg feed. Rations containing sunflower oil without vitamin E supplementation resulted in encephalomalacia of the young chickens. Vitamin deficiency increased triiodine thyronine content in the blood plasma. Antibody formation after the application of human gamma globulin did not depend on the vitamin E supply studied. A vitamin E requirement of young chickens and young hens of the laying species of less than 1 mg/kg mixed feed is derived from the results.  相似文献   

3.
A randomized, blocked 23 factorial experiment was conducted with 48 pigs from sows fed a diet low in selenium and vitamin E. From 3 to 12 weeks of age the piglets were kept in single pens and fed a basic diet consisting mostly of barley, dried skim milk, soybean meal and dried yeast, and containing 55 µg selenium and 3 mg vitamin E per kg. The treatment factors — i.e. feed supplements — were 2 levels of Se (nil, 60 µg/kg), 2 levels of vitamin E (nil, 50 mg/kg), and 2 levels of the feed antioxidant ethoxyquin (nil, 150 mg/kg). Blood samples, collected at termination of the experiment, were examined for glutathione peroxidase activity (GSH-Px) and resistance against erythrocyte lipid peroxidation (ELP) to evaluate Se and vitamin E status, respectively. Analysis of variance showed the GSH-Px activity to be litter-dependent (P < 0.001) and influenced by selenium supplementation (P < 0.001) but not by the other supplements or by interactions between supplements. Resistance against ELP was influenced only by vitamin E supplementation (P < 0.001). GSH-Px and ELP thus seem to be valuable and simple methods for evaluating, respectively, Se status and vitamin E status in growing pigs.  相似文献   

4.
Abstract

The influence of vitamin E supplementation on blood serum vitamin E concentration as well as the seasonal variation of serum vitamin concentration was investigated. Forty horses were divided into a control and three vitamin E supplementation (experimental) groups. The levels of the vitamin E supplementation in the three experimental groups were 1, 3 or 5 mg/kg body weight (bwt) per day. The vitamin was dosed orally. The supplementation was started either at the beginning or in the middle of the indoor (winter) feeding period. There was seasonal variation in the serum vitamin E content; the serum vitamin concentration increased during the grazing and decreased during the indoor feeding period. The basal feeding and a daily supplement of 1 mg/kg bwt were not adequate to maintain or increase the serum vitamin E concentration during the indoor feeding period in an exercising horse. The minimum daily intake of vitamin E would seem to be greater than 1.5 mg/kg bwt. It is suggested that a daily supplement of 3 to 5 mg/kg bwt may be required by horses in training to increase the serum vitamin E levels. This amounts to 1500–2500 mg/day for a horse weighing 500 kg. Supplementation should be started at the beginning of the indoor feeding period.  相似文献   

5.
1. The effects of selenium and vitamin E supplementation of the maternal diet on their transfer to the egg yolk and tissues of the newly hatched chick and on the development of the antioxidant system in the chick liver in early postnatal life were investigated. 2. One hundred Cobb broiler breeder hens were divided into 10 equal groups and housed in pens at 25 weeks of age. Each hen received 1 of the treatment diets which included 0.2 or 0.4 mg/kg selenium, 40, 100, 200 mg/kg vitamin E or their combination. After 6 weeks, the hens were artificially inseminated once per week. From week 8, eggs were collected and placed in an incubator. After hatching, chicks from each group were reared (under standard commercial conditions) to 10 d of age. The chicks were fed on a standard starter commercial broiler diet. At the time of hatching, and at 5 and 10 days old, 4 chicks from each group were sacrificed and blood, liver and brain were collected for the subsequent biochemical analyses. 3. The inclusion of organic selenium or vitamin E in the commercial diet significantly increased their concentration in the egg and in the liver of 1-d-old chicks obtained from the eggs enriched with these substances. A positive effect of such dietary supplementation was seen at d 5 and d 10 of postnatal development. 4. There was a positive effect of selenium supplementation of the maternal diet on glutathione concentration in the liver of 1-d-old and 5-d-old chicks. A combination of a dietary selenium supplementation with high vitamin E doses further increased glutathione concentration in the liver. Dietary selenium supplementation significantly increased selenium-dependent glutathione peroxidase (Se-GSH-Px) activity in the liver of the 1-d-old and 5-d-old chicks and decreased liver susceptibility to peroxidation. 6. It is concluded that the nutritional status of the laying hen determines the efficiency of the antioxidant system throughout embryonic and early postnatal development of the offspring.  相似文献   

6.
1. The effects of selenium and vitamin E supplementation on some immune parameters were investigated in broilers. 2. Broiler chicks were fed on maize-soybean diets with different concentrations of vitamin E (0-200 mg/kg) and selenium (0-0.2 mg/kg diet) either alone or in combinations from 1 to 42 d of age. 3. Chicks were immunised against Newcastle disease virus (NDV) vaccine at 21 d of age and haemagglutination inhibition (HI) titres were determined after 10 d. 4. Chicks receiving supplements of 200 mg vitamin E/kg and 0.2 mg selenium/kg produced significantly higher HI antibody titres. This was associated with an increased serum concentration of total immunoglobulins and circulatory immune complexes. 5. The chicks given 200 mg vitamin E/kg and 0.2 mg selenium/kg had significantly heavier spleen and bursa. 6. These results suggested that vitamin E and selenium have synergistic effects on immune responses.  相似文献   

7.
在3×5的两因子试验中,研究了不同添加水平维生素E(4、14和24mg/kg)与有机和无机硒(亚硒酸钠0.20、0.35和0.50mg/kg,酵母硒0.35和0.50mg/kg)对褐壳蛋种母鸡在20~40周龄阶段生产繁殖性能和有关血液指标的影响。结果表明,产蛋母鸡的采食量、产蛋率、蛋重、产蛋量、饲料转化率和死淘率在3个维生素E添加水平间无差异;除了添加酵母硒0.50mg/kg组蛋鸡的蛋重和产蛋量显著低于酵母硒0.35mg/kg组以外,其它各项生产性能指标在各硒处理组间差异不明显。维生素E和硒可增加彼此在血液中的存留;当日粮中只补加维生素E4mg/kg时,将日粮亚硒酸钠硒添加水平由0.20mg/kg提高到0.35~0.50mg/kg可使血浆α-生育酚含量由0.50μg/ml上升到接近1.0μg/ml左右。  相似文献   

8.
Cottonseed meal (CSM) that contained a high concentration of free gossypol was inadvertently used as a protein supplement, without appropriate iron supplementation, for a swine herd in Illinois. Fifty percent of 300 grower and finishing swine died, and an additional 20% became ill during a 4- to 6-week period. Clinical signs included respiratory distress and abdominal distention. At necropsy, the hearts were diffusely pale, flaccid, and rounded because of dilatation of all 4 chambers, the livers were large and congested, and hydropericardium, hydrothorax, and ascites were evident. Histologic changes consisted of diffuse myocardial fiber atropy with perinuclear vacuolation, and multifocal myocardial and skeletal muscle necrosis. Changes in the liver included marked centrilobular congestion, loss of hepatocytes, and fatty degeneration. Differential diagnoses included monensin, selenium, and gossypol toxicoses, and vitamin E/selenium deficiency. Analyzed feed samples did not contain monensin. Feed selenium concentrations ranged from 428 to 1,513 micrograms/kg, and iron concentrations from 160 to 180 mg/kg. Cottonseed meal (3 to 10%) was detected by feed microscopy. A sample of the 40% protein supplement contained 19% CSM and 1,300 mg of free gossypol/kg, whereas feed samples contained 200 to 400 mg of free gossypol/kg. The history, clinical signs, pathologic findings, and feed analyses were compatible with a diagnosis of gossypol toxicosis. Cottonseed meal, a high-protein supplement used widely in southern United States, may contain gossypol (a polyphenolic binaphthalene pigment), which in its free form is especially toxic to simple-stomached animals. If CSM is used, supplementation with ferrous sulfate is recommended at a 1:1 weight ratio with free gossypol, up to 400 mg of FeSO4/kg.  相似文献   

9.
A randomized blocked factorial experiment was conducted with 90 young pigs. From 4 to 11 weeks of age the pigs were kept in individual pens and fed a selenium supplemented basal diet consisting mostly of propionic acid treated barley, soybean meal and dried skim milk, and containing < 0.5 mg vitamin E per kg. The treatment factors during this period were 3 dietary levels of added vitamin E (nil, 10 and 30 mg/kg) and a 6 % supplement of fresh or oxidized fat (2/3 lard and 1/3 herring oil). From 11 weeks of age until slaughter at 90 kg the pigs received the vitamin E supplements but no fat or dried skim milk. The basal diet for this later experimental period was based on untreated dry barley. Blood samples collected during the period of investigation were examined for vitamin E and for resistance against erythrocyte lipid peroxidation (ELP) in order to evaluate the antioxidant status.Analysis of variance and Student’s t-test on least squares means showed the ELP to be influenced independently by the vitamin E supplement and, during the fat feeding period, by the quality of the fat supplement, with the highest peroxidation resistance (low ELP) in the groups fed fresh fat and a high level of vitamin E. Blood vitamin E level was only influenced — positively — by the vitamin E supplement although variations in the feed vitamin E level below 10–15 mg vitamin E per kg did not result in corresponding variations in measurable blood vitamin E concentrations. In the same low range of vitamin E in the feed there was a statistically significant difference in ELP values between the different vitamin E treatment groups. No clinical manifestations of selenium-vitamin E deficiency were observed in the pigs.The ELP and the plasma vitamin E levels observed would seem to suggest that a total of 15 mg vitamin E per kg barley-based feed will not always be sufficient for growing pigs.  相似文献   

10.
Effect of selenium on sheep lymphocyte responses to mitogens   总被引:1,自引:0,他引:1  
The effect of selenium (Se) on sheep lymphocyte response to mitogens was studied. In an indoor experiment lambs were fed a basal diet containing 0.13 mg Se kg-1, and supplemented with, respectively, 0.1 or 0.5 mg Se kg-1, either as sodium selenite or as selenomethionine. Enhancement of the proliferative response of lymphocytes to phytohaemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A was found in lambs following selenium supplementation at the lower levels. The highest dietary selenium content, however, induced decreased mitogen response. Transient increases in lymphocyte response to PHA and PWM by ewes supplemented with selenium was demonstrated in one field study and a combined effect of selenium and vitamin E was seen in another. There was no stimulatory effect on the mitogen response of lymphocytes from sheep supplemented with dietary vitamin E alone.  相似文献   

11.
杜洛克母猪生长、肥育阶段补充维生素E和维生素C的效果   总被引:1,自引:0,他引:1  
在生长猪日粮(含添加剂VE 7 IU/kg)中添加VE 50IU/kg和VC 100 mg/kg,对杜洛克生长母猪的生产性能无明显影响,肥育猪日粮(含添加剂VE 4.9 IU/kg)中添加VE 25 IU/kg和VC 50 mg/kg,可提高日增重8.6%,单位增重的饲料消耗下降11. 7%。表明杜洛克母猪肥育日粮的VE和VC水平可能应高于生长阶段。  相似文献   

12.
1. This study compared the effect of dietary supplementation with organic or inorganic selenium (Se) sources plus control amounts or large amounts of vitamin E (alpha-tocopherol acetate) in broilers raised at control (20 to 24 degrees C) or low (14.5 to 16.8 degrees C) temperatures after 2 weeks of age. 2. The following dietary treatments were used from one day old. Diet 1, the control diet, comprised a commercial diet containing 0.15 mg/kg inorganic Se and 50 mg vitamin E/kg feed. Diet 2 was the same as diet 1, supplemented with 0.15 mg/kg inorganic Se. Diet 3 was the same as diet 2 but was supplemented with 200 mg/kg vitamin E. Diet 4 was the same as diet 1, but inorganic Se was replaced with 0.30 mg/kg organic Se. Diet 5 was the same as diet 4, supplemented with 200 mg/kg vitamin E. 3. Low temperature reduced the growth rate of broilers; however, at 6 weeks, there were no differences in the body weights of birds fed on organic Se supplemented diets housed at low or control temperature. The feed conversion ratio was significantly affected by low temperature but not by diet. The heterophil/lymphocyte ratio was higher in chicks after one week in the cold, indicating mild stress. Blood triiodothyronine levels were significantly higher in birds after 1 and 4 weeks in the cold but thyroxin was not affected. 4. Organic Se supplementation increased relative lung weight at the control temperature, which might lead to greater respiratory capacity. Relative spleen weight significantly decreased in broilers fed diets supplemented with inorganic Se under cold conditions, a possible indication of chronic oxidative stress. 5. At the low temperature, supplementation with organic Se alone, or with inorganic Se and vitamin E increased glutathione peroxidase (GSHPx) activity and glutathione (GSH) concentration in the liver of broilers, which may indicate increased activity of birds' antioxidant defence against suboptimal environments.  相似文献   

13.
试验探讨维生素E对免疫抑制蛋鸡生产性能和蛋品质的调控效应,选取健康的尼克褐产蛋鸡270只,随机等分为5组,每组3个重复,每重复18只。第Ⅰ组为对照组,第Ⅱ~Ⅴ组为免疫抑制组,对第Ⅱ~Ⅴ组鸡在试验第5、6、7 d进行腿部肌肉注射80 mg/kgBW环磷酰胺(CTX),第Ⅰ组注射等量生理盐水。Ⅰ、Ⅱ组饲喂基础日粮(维生素E含量44.59 mg/kg),Ⅲ、Ⅳ、Ⅴ组在基础日粮中分别添加50、100、200 mg/kg维生素E。结果显示:①免疫抑制产蛋鸡的产蛋率、平均蛋重和平均日采食量显著降低(P<0.05),破蛋率显著升高(P<0.05)。基础日粮中添加50 mg/kg维生素E显著提高免疫抑制产蛋鸡的产蛋率、平均蛋重和平均日采食量(P<0.05),但仍低于对照组Ⅰ(P<0.05);显著降低料蛋比和破蛋率(P<0.05)。②免疫抑制产蛋鸡的蛋壳厚度、蛋壳强度和哈氏单位显著降低(P<0.05),蛋形指数和蛋黄颜色无显著改变(P>0.05);50 mg/kg维生素E添加量显著提高免疫抑制产蛋鸡的蛋壳厚度、蛋壳强度和哈氏单位(P<0.05),与对照组Ⅰ无显著差异(P>0.05)。因此,免疫抑制显著降低产蛋鸡的生产性能和蛋品质;基础日粮中添加维生素E对免疫抑制蛋鸡的生产性能和蛋品质具有明显的调控效应,且以50 mg/kg添加量效果较好。  相似文献   

14.
本试验旨在研究饲粮核黄素水平对15~42日龄北京鸭生长性能和血浆生化指标的影响。采用单因子完全随机试验设计,选取288只体重相近的15日龄雄性北京鸭,随机分为6个组,每组6个重复,每个重复8只鸭。对照组饲喂基础饲粮(核黄素水平为1.38 mg/kg),试验组分别饲喂在基础饲粮中添加1.00、2.00、3.00、4.00和5.00 mg/kg核黄素的试验饲粮。试验期为28 d。结果表明,与对照组相比,饲粮中添加核黄素显著提高了15~42日龄北京鸭的平均日增重和平均日采食量(P0.05),显著提高了血浆核黄素含量(P0.05),显著降低了血浆谷丙转氨酶活性和甘油三酯含量(P0.05)。由此可见,饲粮中添加核黄素可显著提高15~42日龄雄性北京鸭的生长性能和血浆核黄素含量。以平均日增重、平均日采食量、料重比和血浆核黄素含量为评价指标,采用折线模型估测15~42日龄雄性北京鸭核黄素需要量为2.24~2.66 mg/kg。  相似文献   

15.
半胱胺对生长肥育猪后期生长性能的影响   总被引:3,自引:0,他引:3  
选用60头体重约60kg左右的三元杂交(杜×长×大)商品代瘦肉型肥育猪,随机分为试验组和对照组两组,每组又设三个重复,每个重复10头猪。试验组饲喂添加200g/吨半胱胺含量为50%的“大丰收”(由杭州某饲料有限公司提供)的日粮,对照组日粮不添加大丰收。进行为期41天的对比试验。试验结果显示:同对照组相比试验组平均日增重、饲料转化效率、瘦肉率和胴体品质均得到不同程度的提高和改善;而且在外观上也得到明显的改善;猪后臀丰满、皮红毛亮、肌肉发达。  相似文献   

16.
本试验旨在研究饲粮中硒添加水平对产蛋高峰期临武鸭产蛋性能、蛋品质、血清抗氧化指标和蛋硒含量动态变化的影响。试验选用29周龄体况良好、产蛋率接近的正处于产蛋高峰期的临武鸭200只,随机分为5组,每组5个重复,每个重复8只。对照组饲喂基础饲粮(不额外补充硒源,实测硒含量为0.15 mg/kg)45 d,Ⅰ组、Ⅱ组、Ⅲ组、Ⅳ组分别在基础饲粮中以酵母硒形式添加0.10、0.20、0.40、0.80 mg/kg的硒,饲喂35 d添加硒饲粮后改喂基础饲粮10 d。结果显示:1)饲粮中硒添加水平对蛋鸭产蛋性能和蛋品质各指标均无显著影响(P0.05)。2)与对照组和Ⅰ组相比,饲粮中添加0.40和0.80 mg/kg的硒可以显著提高蛋鸭血清中硒和谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P0.05),显著降低血清中丙二醛(MDA)含量(P0.05)。3)随试验天数的增加,对照组和Ⅰ组蛋硒含量无显著变化(P0.05),而Ⅱ组、Ⅲ组、Ⅳ组蛋硒含量均呈现先升高后降低的趋势,且均在试验第9天达到峰值。试验第7、9、13、17、21、28和35天,Ⅱ组、Ⅲ组、Ⅳ组蛋硒含量显著高于对照组和Ⅰ组(P0.05);停喂添加硒饲粮的第1~4天,Ⅱ组、Ⅲ组、Ⅳ组蛋硒含量仍显著高于对照组(P0.05);停喂添加硒饲粮的第5~8天,Ⅲ组和Ⅳ组蛋硒含量显著高于对照组(P0.05);停喂添加硒饲粮的第9天,各试验组蛋硒含量均衰减至与对照组相同水平(P0.05)。由此可见,饲粮中添加不同水平的酵母硒形式的硒对产蛋高峰期临武鸭的产蛋性能和蛋品质均无不良影响。饲粮中添加0.40和0.80 mg/kg酵母硒形式的硒可以提高产蛋高峰期临武鸭的蛋硒含量、血清硒含量和抗氧化能力,延长蛋硒的存留时效。综合考虑各因素,产蛋高峰期临武鸭饲粮中硒(以酵母硒形式添加)的适宜添加水平为0.40 mg/kg。  相似文献   

17.
试验在肉鸡日粮中添加维生素E(VE)、有机硒,旨在探讨有机硒与VE对肉仔鸡肉质性状、血液生化指标及抗氧化性能的影响。将280只1日龄AA肉仔鸡随机分为7组,每组40只,1组不添加有机硒和VE,2组添加100 IU/kg VE,3组添加0.15 mg/kg有机硒和100 IU/kg VE,4组添加0.30 mg/kg有机硒和100 IU/kg VE,5组添加200 IU/kg VE,6组添加0.15 mg/kg有机硒和200 IU/kg VE,7组添加0.30 mg/kg有机硒和200 IU/kg VE。结果显示,添加有机硒与VE对肉仔鸡的全净膛率、屠宰率、胸肌率影响显著(P< 0.05),对半净膛率、腿肌率无显著影响(P> 0.05);添加0.15 mg/kg有机硒、100 IU/kg VE可以降低肉仔鸡的滴水损失、蒸煮损失、L*值及pH,其中蒸煮损失、L*值与对照组间差异极显著或显著(P< 0.01;P< 0.05),但对剪切力、a*、b*值却无显著影响(P> 0.05);能够显著或极显著降低血液胆固醇(CHO)及丙二醛(MDA)含量(P< 0.05;P< 0.01),提高血糖(GLU)、总抗氧化能力(T-AOC)与谷胱甘肽过氧化物酶(GSH-Px)含量,其中GLU与对照组间差异显著(P< 0.05),GSH-Px与对照组间差异极显著(P< 0.01),但对过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量无显著差异(P> 0.05)。  相似文献   

18.
Selenium may be related to the hepatic metabolism of the coumarin compounds aflatoxin B1 and warfarin. Selenium evidently increased the pharmacologic activity of warfarin, probably due to a displacement of warfarin from albumin by selenium, the close relationship among selenium, vitamin E, and sulphur-containing groups (eg, glutathione), or the antioxidant effect of selenium. A diet containing selenium in a concentration of 2.5 mg/kg of feed was protective against the toxic effects of both coumarins in pigs given 4 daily oral doses of 0.2 mg/kg of body weight. Selenium, as glutathione peroxidase, at least in part, protects the hepatic cells against the toxic effects of aflatoxin B1 and warfarin. The protection was demonstrated by alteration of clinical responses and hematologic (prothrombin times), electrophoretic, and clinical chemistry values. It also was demonstrated that selenium at 2.5 mg/kg of feed does not produce toxic effects; however, dietary selenium at a concentration of 5 mg/kg (and in the presence of both toxic agents) was toxic for young pigs within the 3-week experimental period. Warfarin was more active as an anticoagulant than aflatoxin B1.  相似文献   

19.
硒与维生素E对鸡马杜霉素急性中毒的保护作用   总被引:3,自引:0,他引:3  
在实验条件下用7mg/kg bw.剂量的马杜霉不诱发鸡急性中毒,处理组鸡在给马杜霉素前给予硒(0.2mg/kg bw.剂量,内服)-维生素E(每kg饲料含200mg)7d,在给马杜霉素后给予硒(0.2mg/kg bw.剂量,内服)-维生素E(200mg/kg bw.剂量,肌注)4d,中毒组在给马杜霉素前后不给任何硒和维生素E,对照组既不给马杜霉素,也不给硒与维生素E。结果是:处理组鸡的死亡率显著低于中毒组(P<0.05),且病鸡恢复比中毒组快。处理组的肝脏、心肌、骨骼肌的出血与变性比中毒组轻,但处理组与中毒组的肾脏的出血与变性的程度相似;处理组的血清GOT值、肝脏的脂质过氧化物的含量、肝脏过氧化氢酶的性均显著低于中毒组;但处理组的血清CK值与中毒组相比无显著性差异。结果表明硒与维生素E对鸡马杜霉素急性中毒有较好的保护作用。  相似文献   

20.
Moderate to extremely low levels of selenium in hay were related to marginally deficient levels of selenium in sera from cattle in the Windermere Valley of southeastern British Columbia. Deficiency was most pronounced in cattle fed local hay during the winter or grazed on fertilized/irrigated pastures. Cattle on range had adequate levels of serum selenium. When sodium selenite was fed at 1.0 and 3.5 mg per head per day, serum selenium levels increased commensurate with the dose. Clinical observations indicated that a variety of clinical problems disappeared after injection or supplementation with selenium/vitamin E.  相似文献   

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