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1.
Our objectives were to compare the effects of sources of supplemental N on ruminal fermentation of dried citrus pulp (DCP) and performance of growing steers fed DCP and bahiagrass (Paspalum notatum) hay. In Exp. 1, fermentation of DCP alone was compared with that of isonitrogenous mixtures of DCP and solvent soybean meal (SBM), expeller soybean meal (SoyPLUS; SP), or urea (UR). Ground (1 mm) substrates were incubated in buffered rumen fluid for 24 h, and IVDMD and fermentation gas production kinetics and products were measured. Nitrogen supplementation increased (P < 0.10) ruminally fermentable fractions, IVDMD, pH, and concentrations of NH3 and total VFA, but reduced the rate of gas production (P < 0.10) and the lag phase (P < 0.01). Supplementation with UR vs. the soy-based supplements increased ruminally fermentable fractions (P < 0.05) and concentrations of total VFA (P < 0.10) and NH3 (P < 0.01), but these measures were similar (P > 0.10) between SBM and SP. In Exp. 2, 4 steers (254 kg) were fed bahiagrass hay plus DCP, or hay plus DCP supplemented with CP predominantly from UR, SBM, or SP in a 4 x 4 Latin square design, with four 21-d periods, each with 7 d for DMI and fecal output measurement. Nitrogen-supplemented diets were formulated to be isonitrogenous (11.9% CP), and all diets were formulated to be isocaloric (66% TDN). Intake and digestibility of DM, N, and ADF were improved (P < 0.05) by N supplementation. Compared with UR, the soy-based supplements led to greater (P < 0.05) DM and N intakes and apparent N and ADF digestibilities. Plasma glucose and urea concentrations increased (P < 0.10) with N supplementation and were greater (P < 0.01) for the soy-based supplements than for UR. Intake, digestibility, and plasma metabolite concentrations were similar (P > 0.1) for SBM and SP. In Exp. 3, 24 steers (261 kg) were individually fed bahiagrass hay plus DCP (control), or hay plus DCP supplemented with CP predominantly from UR or SBM. Over 56 d, DMI and ADG were greatest (P < 0.05) in steers fed SBM. Nitrogen supplementation increased (P < 0.05) DMI, ADG, and G:F. However, SBM supplementation produced greater (P < 0.05) DMI and ADG and similar (P > 0.05) G:F compared with UR supplementation. We conclude that supplemental N is important to optimize ruminal function and performance of growing steers fed forage diets supplemented with DCP. Diets with supplemental N mainly from SBM improved diet digestibility and animal performance beyond that achieved by UR.  相似文献   

2.
Two 160-d feedlot experiments, each consisting of 20 Angus-Hereford steers (216 +/- 5 kg BW, Exp. 1; 258 +/- 5 kg BW, Exp. 2) and 20 Angus-Hereford heifers (208 +/- 5 kg BW, Exp. 1; 236 +/- 5 kg BW, Exp. 2), were used to investigate the effects of supplementing diets with either roasted soybeans (RSB, roasted at 127 degrees C for 10 min) or soybean meal (SBM) and implanting or not implanting with an estrogenic growth promoter (SYN; Synovex-S, 20 mg of estradiol benzoate plus 200 mg of progesterone or Synovex-H, 20 mg of estradiol benzoate plus 200 mg of testosterone) on performance. The cattle were fed a basal diet of 15% orchardgrass silage, 15% corn silage, and 70% corn-based concentrate. Treatments were 1) no SYN and fed a SBM-supplemented diet, 2) no SYN and fed a RSB-supplemented diet, 3) SYN and SBM, and 4) SYN and RSB. Cattle in the SYN groups were reimplanted at 80 d. Four additional Angus-Hereford steers were used in a digestion and nitrogen balance experiment conducted during the first half of Exp. 1. For the total 160-d feedlot experiments, DMI for RSB compared with SBM was lower (P < .01; 8.5 vs 9.2 kg/d, SEM = .07) and ADG/DMI tended to be higher (P < .10; 165 vs 157 g/kg, SEM = 1.3). Final BW of steers fed RSB was similar (P > .10) to that of steers fed SBM (473 vs 478 kg, SEM = 5.6), as was ADG (1.39 vs 1.43 kg/d, SEM = .02). Dry matter intake for SYN-implanted steers was higher (P < .01) than for steers not implanted (9.2 vs 8.5 kg/d). Likewise, final BW (491 vs 460 kg) and ADG (1.49 vs 1.33 kg/d) were higher (P < .01), and ADG/DMI (166 vs 157 g/kg) tended to be higher (P < .10), for SYN-implanted steers than for steers not implanted. During the more rapid muscle growth period (0 to 80 d), DMI for RSB compared with SBM was lower (P < .01; 7.8 vs 8.6 kg/d, SEM = .07) and ADG/DMI was similar (P > .10; 181 vs 172 g/kg, SEM = 1.8). Dry matter intake for SYN-implanted steers was higher (P < .05) than for steers not implanted (8.4 vs 8.0 kg/d), as was ADG/DMI (P < .01, 182 vs 171 g/kg). During this more rapid growth period, the supplement x implant interaction for ADG was significant (P < .05; 1.35, 1.36, 1.59, and 1.44 kg/d for Treatments 1, 2, 3, and 4, respectively, SEM = .04). There were no differences in digestibilities or N balance. The results suggest that there is no improvement in performance under feedlot conditions when RSB replaces SBM in the diet of beef cattle, and, in young cattle, RSB may reduce the response expected by an estrogenic growth promoter.  相似文献   

3.
Six ruminal-cannulated nonlactating Holstein Friesian cows (mean body weight:660 ± 42.9 kg) were used to investigate the effect of soybean meal (SBM) supplementation on voluntary rice straw (RS) intake, feed particle size reduction, and passage kinetics in the rumen. They were allocated to two dietary treatments: RS alone or RS supplemented with SBM. Voluntary dry matter intake of RS and total tract fiber digestibility was increased by SBM supplementation (p < 0.05). Supplementation with SBM decreased rumination time per dietary dry matter (DM) and neutral detergent fiber (NDFom) intake (p < 0.01). Particle size distribution in the rumen and total ruminal NDFom digesta weights were not affected by SBM supplementation. However, the disappearance rates of total digesta and large and small particles from the rumen were increased by SBM supplementation (p < 0.01). Moreover, SBM supplementation increased the rate of size reduction in ruminal particles (p < 0.05). In situ disappearance of DM and NDFom of RS in the rumen was greater in SBM-supplemented cows than in nonsupplemented cows (p < 0.05). This study clearly showed that increased ruminal RS particle size reduction, passage, and fermentation due to SBM supplementation accelerated the RS particle clearance from the rumen and resulted in increased voluntary RS intake of dairy cows.  相似文献   

4.
Four experiments were conducted to determine the effect of adding corn gluten mean (CGM) or soybean meal (SBM) at 24- or 48-h intervals to diets based on corn stalks. In each experiment corn stalks was the primary diet ingredient fed to wethers or steers. Monensin was also fed to determine whether its effects on ruminal fermentation would improve the efficiency of N utilization under these conditions. Evaluation criteria included ruminal fermentation characteristics, DM intake and utilization, N balance in sheep, and steer feedlot performance. Ruminal ammonia nitrogen (NH3 N) concentrations measured over time were higher (P < .05) when diets contained SBM. Diet did not influence (P > .10) total VFA concentrations in ruminal fluid. Differences in diurnal shifts in ruminal NH3 N and total VFA due to protein source resulted in diet x hour interactions (P < .05). Dry matter intake response to protein source and frequency of supplement feeding was variable. Dry matter digestibility and nitrogen digestibility were not affected (P > .10) by protein source or feeding interval. The 48-h interval feeding of CGM was favorable compared with 24-h interval feeding (P < .05). The opposite response occurred with SBM, resulting in a diet x feeding interval interaction (P < .05). Nitrogen retention was greater (P < .05) when CGM was fed and with alternate day feeding. Diets that contained CGM supported higher (P < .05) ADG and gain/feed than diets that contained SBM when fed to steer calves. Alternate day feeding of supplements that contained monensin was detrimental to steer performance under the conditions of these experiments. Corn gluten meal is an effective substitute for SBM when alternate day protein supplementation is practiced.  相似文献   

5.
The objective of two experiments was to correlate plasma levels of urea N (PUN) and the percentage of urine N in the form of urea (UUN) to weight gain in response to different dietary protein regimens for growing Angus steers. In Exp. 1, 60 steers (302 kg BW) were assigned to various levels of dietary N (control plus supplemental N to provide from 100 to 400 g more crude protein daily) within two sources of supplemental N (soybean meal [SBM] or a mixture of two parts corn gluten meal:one part blood meal [CGM:BM]). In Exp. 2, 27 steers (229 kg BW) were fed two levels of SBM, and half of the steers received growth-promoting implants. Steers were housed in groups of 12 and fed individually for 84 d in both experiments. Corn silage was fed at a restricted rate to minimize orts. Jugular blood and urine samples were collected during the experiments. In Exp. 1, maximal ADG of steers fed SBM (1.0 kg) was reached with 671 g/d total crude protein, or 531 g/d metabolizable protein. Maximal ADG of steers fed CGM:BM (0.91 kg) was reached with 589 g/d total crude protein, or 539 g/d metabolizable protein. The DMI was higher (P < 0.07) for steers fed SBM (6.37 kg/d) than for steers fed CGM:BM (6.14 kg/d). Increasing ruminal escape protein from 36% (SBM) to 65% (CGM:BM) of CP decreased (P < 0.05) endogenous production of urea, as evidenced by lower concentrations of urea in blood and lower UUN. In Exp. 2, increasing supplemental protein from 100 to 200 g/d increased (P < 0.05) ADG and PUN. Implants lowered (P < 0.05) UUN, particularly at the higher level of supplemental protein. Protein supplementation of growing steers can be managed to maintain acceptable ADG yet decrease excretion of urea in the urine.  相似文献   

6.
Six ruminally cannulated Angus-cross steers (362 kg) were used in a replicated 3 x 3 Latin square design to determine effects of supplementing Maillard reaction products (MRP) on acid-resistant E. coli and coliform populations. Steers were fed roughage-based diets supplemented (DM basis) with either 10% soybean meal (SBM), 10% nonenzymatically browned SBM (NESBM), or 10% SBM top-dressed with 45 g of a lysine-dextrose Maillard reaction product (LD-MRP). Equal weights of dextrose, lysine hydrochloride, and deionized water were refluxed to produce the LD-MRP. The NESBM was manufactured by treating SBM with invertase enzyme, followed by heating to induce nonenzymatic browning. Steers were allowed slightly less than ad libitum access to diets fed twice daily and were adapted to their respective treatments within 10 d. On d 11, ruminal and fecal samples were collected at 0, 2, 4, 6, 8, and 12 h after feeding from each of the steers and transported to the laboratory for microbial analysis. Ruminal samples and feces were analyzed for pH and VFA, and both ruminal fluid and feces were tested for acid-resistant E. coli and total coliforms by incubating samples in tryptic soy broth adjusted to pH 2, 4, and 7. Ruminal pH and total VFA concentrations did not differ among treatments. The molar proportion of ruminal acetate was higher (P < 0.05) for steers receiving NESBM than for steers receiving SBM and LD-MRP. At pH 4, steers that received NESBM had lower (P < 0.05) ruminal populations of E. coli and total coliforms than steers that received SBM. No differences were observed for ruminal E. coli and total coliforms at pH 2 and 7. Fecal pH was lower (P < 0.05) for steers fed NESBM than for steers fed SBM or LD-MRP. Molar proportions of fecal acetate were lower (P < 0.05) and proportions of butyrate and isovalerate were higher (P < 0.05) for steers fed NESBM compared with steers fed SBM. Fecal E. coli at pH 4 was lower (P < 0.05) for steers fed NESBM than for steers fed LD-MRP. Fecal E. coli and total coliforms at pH 2 and 7 did not differ among treatments. Dietary MRP had limited effectiveness at decreasing acid-resistant coliforms in the rumen and feces of cattle. Acid resistance in coliforms may depend on protein availability.  相似文献   

7.
Seven cannulated (rumen and duodenal) Angus x Hereford steers (264 +/- 8 kg BW) consuming low-quality forage (5% CP; 61% NDF; 31% ADF) were used to determine the influence of CP degradability and supplementation frequency (SF) on DMI and nutrient digestion. Treatments included an unsupplemented control and degradable intake protein (DIP) or undegradable intake protein (UIP) provided daily, every 3 d, or every 6 d. The DIP treatments (18% UIP) were calculated to provide 100% of the DIP requirement, while the UIP treatments (60% UIP) were provided on an isonitrogenous basis compared with DIP. Forage DMI was not affected by treatment. Total DM and N intake, duodenal N flow, and intestinal N disappearance increased (P < 0.01) with supplementation. Dry matter intake and duodenal N flow responded quadratically (P < 0.04; greatest values on the every-third-day treatments) as SF decreased. However, no differences in N intake or intestinal N disappearance were observed because of CP degradability or SF. Duodenal bacterial N flow and true bacterial N synthesis (g bacterial N/kg of OM truly digested in the rumen) were increased (P < 0.05) with supplementation. Also, duodenal bacterial N flow was greater (P < 0.05) for DIP compared with UIP. Duodenal nonbacterial N flow was increased (P = 0.02) with CP supplementation and for UIP compared with DIP (P < 0.01). Supplemental CP increased (P < 0.01) total tract DM and N digestibility with no difference due to CP degradability or SF. Results suggest CP supplements consisting of 20 to 60% UIP can be effectively used by steers consuming low-quality forage without adversely affecting DMI, nutrient digestibility, or bacterial CP synthesis, even when provided as infrequently as once every 6 d.  相似文献   

8.
Four rumen-fistulated steers averaging 400 kg in body weight were used in a 4 X 4 Latin square arrangement with 18-d periods to investigate the effect of treating soybean meal (SBM) with formaldehyde on nitrogen (N) utilization and ruminal fermentation. Experimental diets, on a dry matter basis, consisted of 42% corn silage, 48.5% cracked corn-mineral mixture and 9.5% SBM treated with 0, .3, .6 or .9% formaldehyde by weight. Dry matter and organic matter digestibilities were not affected by treatment. Formaldehyde treatment of SBM resulted in a linear decrease in N digestibility (P less than .005) and urinary N excretion (P less than .01) and a quadratic increase (P less than .05) in N retention. The depression in apparent N digestibility was small when SBM was treated with .3% formaldehyde. This level of formaldehyde treatment also had little effect on in vitro enzymatic hydrolysis of SBM. Ruminal ammonia-N concentrations were lower (P less than .05) in steers fed formaldehyde-treated SBM. Ruminal pH was lower (P less than .05) at 6 and 8 h postfeeding while volatile fatty acid concentrations were higher (P less than .05) at 8 and 12 h postfeeding for steers fed untreated SBM. Propionic acid (mol/100 mol) decreased linearly (P less than .05) with increasing level of formaldehyde treatment. Urea-N concentrations in plasma were decreased (P less than .001) and plasma-free essential amino acid concentrations were increased (P less than .10) by formaldehyde treatment. Ruminal disappearance of N from polyester bags containing the SBM supplements was greatly reduced (P less than .005) by formaldehyde treatment.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

9.
Experiments (Exp) I and II were conducted to compare raw whole soya beans (WSB), roasted (rWSB) or other protein sources as supplements of low‐quality forages fed ad libitum to beef cattle, upon DM intake (DMI), ruminal and blood parameters, and animal performance. Exp I: treatments for wheat straw fed to four ruminally cannulated steers were (i) Control‐WS: no supplement; (ii) WSB‐WS: whole soya beans; (iii) rWSB‐WS: roasted WSB; and (iv) SBM‐WS: soybean meal–wheat midds mixture; all fed at 1.4 kg DM/day. Exp II: 12 steers grazed deferred grain sorghum (DS) receiving these treatments: (i) Control‐DS: no supplement; (ii) WSB‐DS: 1.26 kg DM/day whole soya beans; and (iii) SFM‐DS: 1.35 kg DM/day of sunflower meal. In Exp I, WS DMI resulted 47, 52 and 41% greater for WSB‐WS, rWSB‐WS and SBM‐WS, respectively, than Control‐WS (< .05). In Exp II, the DMI of DS was unaffected by supplementation; a substitution of DS by supplement was found for WSB‐DS (< .05); however, total diet and digestible DMI increased with supplementation (< .05). Rumen pH in Exp I remained unaffected by supplementation, but N‐NH3 as well as blood urea‐N in Exp II increased (< .05). In Exp II, average daily weight gains improved similarly with both supplements compared with Control‐DS. Additionally, feed‐to‐gain ratio decreased (p < .05), being lower for WSB‐DS (8.3) vs. SFM‐DS (9.9). Roasting effects of WSB as a supplement for low‐quality forages were not detected, and all protein sources increased total diet DMI and forage utilization. Only moderate cattle weight gains could be expected for unsupplemented DS.  相似文献   

10.
Six Japanese Black (Wagyu) steers (average initial weight 467 ± 45 kg) fitted with a ruminal cannula were used in a split‐plot design experiment comprising a 3 × 3 Latin square design (whole‐plot) and a randomized block design (subplot). The whole‐plot treatments were three different feeding levels of urea‐treated potato pulp (PP) silage‐based concentrate: 1.00%, 1.75% and 2.50% of body weight (BW) (on a dry matter (DM) basis). The subplot treatments consisted of the concentrate formulated to contain either soybean meal (SBM) as a rapidly rumen‐degraded protein source or corn gluten meal (CGM) as a slowly degraded protein source. Dry matter intake tended to be lower (P = 0.071) for CGM (8.9 kg/day) than for SBM (9.4 kg/day). Protein sources had no significant effect on digestibility and in situ degradation. Ruminal ammonia nitrogen (NH3‐N) was lower (P = 0.033) for CGM (7.5 mg/dL) than for SBM (9.5 mg/dL). Protein sources did not affect ruminal pH and the total volatile fatty acids (VFA) concentrations. The molar proportions of ruminal acetate and valerate were higher (P = 0.032) for CGM than for SBM. The maximum daily intake of the PP silage‐based concentrate expressed as a percentage of BW was approximately 1.4% of BW. Dry matter intake was higher (P = 0.046) for steers fed at 1.0% of BW of the PP silage‐based concentrate than for steers fed at 1.75% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate had no effect on DM and nutrients digestibility, except for crude protein (CP) digestibility. CP digestibility tended to be lower (P = 0.071) for steers fed at 1.75% of BW of the PP silage‐based concentrate than for steers fed at 1.0% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate also did not affect the in situ degradation parameter of hay and PP silage. The feeding levels of the PP silage‐based concentrate did not affect ruminal pH, NH3‐N and total VFA concentrations. The molar proportion of acetate was highest for steers fed at 1.0% of BW of the concentrate. In conclusion, in the urea‐treated PP silage‐based concentrate, CGM seems to be more effective than SBM for stabilizing the ruminal NH3‐N concentration and to be advantageous for fiber digestion in the rumen. The feeding levels of the PP silage‐based concentrate did not change the amount of VFA production in the rumen and the DM digestibility.  相似文献   

11.
To establish the effect of sward height (SH) and concentrate supplementation on performance of grazing cattle, 24 crossbred Angus beef cows (535 kg BW) and calves (114 kg BW) were grouped by weight and calving date. They were randomly assigned to two SH treatments, either 4 to 8 cm or 8 to 11 cm, and fed three levels of supplement, high, low, or none, consisting of 6.24, 3.12, and 0 kg x animal(-1) x d(-1), respectively. The experiment was repeated over three 15-d periods in 1996: May (P1), June/July (P2), and August (P3). No SH x supplement level x period or SH x supplement level interactions (P > 0.10) were evident for responses tested. Cows on lower SH had greater (P < 0.08) DMI but spent an additional 1.3 h/d (P < 0.01) grazing compared with cows on higher SH. Sward height had no influence (P > 0.10) on forage DM digestibility (DMD). Forage DMI, DMD, and grazing time (GT) decreased (P < 0.05) as supplementation increased. Nonetheless, supplemented cows consumed more total DMI (P < 0.08) than unsupplemented cows. Cows consumed 2.4 kg/d more forage DM (P < 0.01) in P1 and P2 than in P3. Cows grazed 1.3 h/d (P < 0.01) less in P1 than in P2 and P3. Grazing efficiency (DMI/h GT) declined as supplementation increased and grazing season advanced to P3 (P < 0.01). Decreased forage DMI and grazing efficiency with increasing supplementation suggests that supplemented cattle should be able to maintain productivity while grazing at SH lower than unsupplemented cattle.  相似文献   

12.
To evaluate the effects of balancing total diet degradable intake protein with dietary total digestible nutrients (TDN), we conducted two studies during 2 yr with 100 (302 +/- 8 kg initial BW) mixed-breed yearling steers and 12 ruminally cannulated steers (526 +/- 28 kg). Steers individually received one of four supplements 5 d/wk while grazing dormant native tallgrass prairie. Supplements included: 1) corn and soybean meal, balanced for total diet degradable intake protein in relation to total diet TDN (CRSBM), 2) corn and soybean hulls, equal in supplemental TDN to CRSBM (CORN), 3) soybean meal, equal in supplemental degradable intake protein to CRSBM (SBM), or 4) a cottonseed hull-based control supplement (CONT). At each feeding (5 d/wk), steers consumed 13.6, 13.6, or 4.2 g of dry matter/kg of body weight, or 178 g of DM, respectively, of supplement. Steers fed CRSBM had greater (P < 0.01) average daily gain than cattle fed CORN or SBM. Feeding soybean meal (CRSBM, SBM) resulted in improved (P < 0.01) efficiency of supplement. Grazing time, intensity, and harvesting efficiency were reduced (P < 0.05) by corn supplementation (CRSBM and CORN), whereas the number of grazing bouts per day was increased (P < 0.08). Intake and digestibility of forage organic matter were reduced (P < 0.01) for steers supplemented with corn (CORN and CRSBM) vs cattle not fed corn (SBM and CONT). Total diet digestibility (P < 0.12) and digestible organic matter intake (P < 0.01) were greater for CRSBM-fed steers than for cattle fed either CORN or SBM. Steers fed CRSBM had greater (P < 0.01) fecal nitrogen and serum insulin than cattle fed CORN or SBM. Corn-fed cattle had lesser (P < 0.01) fecal pH and ADF concentrations than steers not consuming grain. Cattle fed supplements with soybean meal (CRSBM and SBM) had greater (P < 0.01) serum urea nitrogen than steers fed supplements without soybean meal (CORN, CONT). Supplemented steers grazing dormant tallgrass prairie had a greater rate of gain, with the greatest response in animal performance occurring when grain supplements were balanced for total diet degradable intake protein in relation to total diet TDN. These results lead us to suggest that grain-supplemented cattle grazing dormant tallgrass prairie require a balance of total diet degradable intake protein in relation to total diet TDN to optimize animal performance.  相似文献   

13.
Three hundred, day-old broiler chicks, with an average initial weight of 41.8±1.79 g, were used in a 15-day study (10 birds per battery cage) to characterize their performance and fluorine status when dicalcium phosphate (DCP) was replaced by Busumbu rock phosphate (BRP) as the source of phosphorus in the chicks' ration. The treatments comprised a standard ration with BRP replacing 0, 25%, 50%, 75% or 100% of DCP. Replacing DCP with BRP significantly reduced the final weight of the chicks (p<0.01), feed intake (p<0.01), weight gains (p<0.01) and dry matter digestibility (p<0.05) but increased (p<0.05) the feed-to-gain ratio. True phosphorus absorption and the percentage of phosphorus in the tibia were not affected by increasing amounts of BRP in the diet. Increasing levels of BRP in the diet linearly reduced (p<0.01) the percentage bone ash, calcium, Ca:P ratio, ultimate breaking force, bending moment, stress, and modulus of elasticity. Leg stiffness, lameness, reduced feed intake, and a decline in general health were recorded in 10–40% of the chicks on 75% and 100% BRP, respectively. These results suggest that excessive ingestion of fluorine from the BRP caused the reduction in the chicks' performance.  相似文献   

14.
Multiple fecal samples were collected from growing Angus bulls (264 to 419 kg of BW, 3.0 to 11.4 kg/d of DMI) to predict DMI of a corn-silage-based diet. Contemporaneous digestion trials were conducted with the same diet in 12 steers in yr 1 to 3 and bulls in yr 4. Near-infrared spectra from fecal samples (n = 730 from 282 growing bulls, n = 240 from 36 steers and 12 bulls for digestion trials) were obtained from dried and ground fecal samples, and modified partial least squares regression was used to develop equations to predict DMI and DM digestibility (DMD). Although mean predicted DMI of the growing bulls (7.52 ± 0.04 kg/d or 22.4 ± 0.1 g/kg of BW) was within 2% of mean measured DMI (7.63 ± 0.06 kg/d or 22.7 ± 0.1 g/kg of BW), the mean of paired differences within samples (0.11 ± 0.04 kg/d or 0.3 ± 0.1 g/kg of BW) was greater (P < 0.01) than zero. Measured DMD (72.3 ± 0.5%) was identical (P < 0.97) to predicted DMD (72.3 ± 0.5%), and DMD for bulls in the digestion trial did not differ (P < 0.27) from DMD for steers. Prediction of intake requires incorporation of some measured values from the set of fecal samples to be predicted. Lack of similarity between spectra of fecal grab samples from the growing bulls and daily fecal collection of steers and bulls in the digestion trials in this study indicates the need for further verification before prediction of DMD with fecal grab samples.  相似文献   

15.
Twenty-eight Angus (289 +/- 3.8 kg) steers were used in a completely randomized design to evaluate the effect of isocaloric supplementation of 2 different energy sources to steers rotationally grazing tall fescue pastures for 197 d in comparison to positive and negative controls. Steers were supplemented with either corn grain (0.52% BW on a DM basis; PC) or soybean hulls plus corn oil (0.45% BW on a DM basis + 0.10% BW on an as-fed basis; PO) using Calan gates for individual intake measurement. Negative, pasture only (PA), and positive, high-concentrate control diets (85% concentrate:15% roughage on DM basis; C) were also included in the study. Steers on PC, PO, and PA treatments were managed together under a rotational grazing system, whereas C steers were fed a high-concentrate diet for the final 113 d using Calan gates. Forage DMI and apparent DM and NDF digestibility for the grazing treatments were evaluated using Cr(2)O(5) and indigestible NDF as digesta markers. Energy supplementation decreased (P = 0.02) forage DMI (% of BW) with respect to PA, but not (P = 0.58) total DMI. There were no differences (P = 0.53) among grazing treatments on apparent total DM digestibility. However, NDF digestibility was less (P < or = 0.05) in PC than in PO and PA; the latter 2 treatments did not differ (P > 0.05). Overall ADG was greater (P < 0.01) in supplemented, regardless of type, than in nonsupplemented grazing treatments. During the final 113 d, ADG was greater (P < 0.01) in C than in the grazing treatments. Overall supplement conversion did not differ (P = 0.73) between supplement types and was less (P = 0.006) than C. Carcass traits did not differ (P > 0.05) between energy sources. Dressing percentage and HCW were greater (P < 0.01) in supplemented cattle than in PA. Fat thickness and KPH percentage for PA were less (P < 0.05) than for PO but did not differ (P > 0.14) from PC. Marbling score, LM area, and quality grade did not differ (P > 0.05) between grazing treatments. Hot carcass weight for C was heavier (P < 0.001) than for pastured cattle. Quality and yield grades of C carcasses were also greater (P < 0.001) than carcasses from pastured steers. Energy supplementation, regardless of source, to grazing steers increased ADG, dressing percentage, and carcass weight compared with PA steers; however, supplemented steers had less ADG, efficiency, dressing percentage, and carcass weight compared with high-concentrate finished steers.  相似文献   

16.
Eighteen Angus steers (438 +/- 4 kg of BW) were supplemented with varying levels of corn oil (0 g/kg of BW, none; 0.75 g/kg of BW, MED; or 1.5 g/kg of BW, HI) on rotationally stocked, endophyte-free tall fescue to determine the effect of supplemental oil level on in vivo digestibility, intake, performance, and carcass traits. Pelleted cottonseed hulls were used as a carrier for the oil supplements, and all supplements were offered to steers using Calan gate feeders for individual intake determination. On d 49, each steer was dosed with a controlled-release capsule containing chromium sesquioxide, and fecal samples were obtained 12 d later over a 7-d period to estimate fecal output that, with forage, supplement, and fecal indigestible NDF concentration, was used to estimate DMI and in vivo total diet digestibility. Steers were slaughtered at the end of the 116-d grazing period, and carcass data were collected at 24 h postmortem. Total fatty acid intake linearly increased with corn oil supplementation, and forage DMI, total DMI, and total DE intake were linearly decreased (P < 0.01). The decrease in total DMI was reflected in forage substitution rates greater (P < or = 0.01) than 1, with a trend (P = 0.09) for a greater substitution rate in HI than in MED. In vivo DM, OM, and NDF digestibility were linearly decreased (P < 0.01) by corn oil supplementation. Average daily gain and final BW tended (P = 0.09) to increase linearly in response to oil level. Oil conversion (0.36 kg of BW gain/kg of corn oil) was greater (P < or = 0.05) than zero and did not differ (P = 0.15) between MED and HI. Dressing percent (P = 0.09), carcass weight (P = 0.01), and carcass backfat thickness (P = 0.01) increased linearly with oil supplementation. No treatment effect was observed for carcass LM area, KPH percentage, marbling score, or yield grade (P > 0.10). Oil supplementation to grazing steers linearly reduced forage DMI intake; however, animal performance was maintained and tended to be greater for oil-supplemented cattle. Oil supplementation increased carcass fat thickness and weight without altering other carcass quality parameters.  相似文献   

17.
A study was conducted to determine whether bacterial direct-fed microbials (DFM) could be used to minimize the risk of acidosis in feedlot cattle receiving high concentrate diets. Six ruminally cannulated steers, previously adapted to a high concentrate diet, were used in a double 3 x 3 Latin square to study the effects of DFM on feed intake, ruminal pH, and ruminal and blood characteristics. Steers were provided ad libitum access to a diet containing steam-rolled barley, barley silage, and a protein-mineral supplement at 87, 9, and 4% (DM basis), respectively. Treatments were as follows: control, Propionibacterium P15 (P15), and Propionibacterium P15 and Enterococcus faecium EF212 (PE). The bacterial treatments (10(9) cfu/g) plus whey powder carrier, or whey powder alone for control, were top-dressed once daily at the time of feeding (10 g/[steer/d]). Periods consisted of 2 wk of adaptation and 1 wk of measurements. Ruminal pH was continuously measured for 6 d using indwelling electrodes. Dry matter intake and ruminal pH (mean, minimum, hours, and area pH < 5.8 or < 5.5) were not affected by treatment (P > 0.05). However, supplementation with P15 increased protozoal numbers (P < 0.05) with a concomitant increase in ruminal NH3 concentration (P < 0.01) and a decrease in the number of amylolytic bacteria (P < 0.05) compared with the control. Streptococcus bovis, enumerated using a selective medium, was numerically reduced with supplementation of PE. Although blood pH and blood glucose were not affected by DFM supplementation, steers fed PE had numerically lower concentrations of blood CO2 than control steers, which is consistent with a reduced risk of metabolic acidosis. Although the bacterial DFM used in this study did not induce changes in DMI or ruminal and blood pH, some rumen and blood variables indicated that the bacterial DFM used in this study may decrease the risk of acidosis in feedlot cattle.  相似文献   

18.
We conducted two experiments to evaluate the effect of the ionophore laidlomycin propionate (LP) on steer performance and ruminal N metabolism. Experiment 1 was a 91-d growth study evaluating the growth and ruminal characteristics of steer calves consuming supplemental LP. Steers (n = 96; 255 +/- 3 kg; four steers/pen; six pens/treatment) were used in a randomized complete block design with a 2 x 2 factorial arrangement of treatments consisting of two levels of dietary CP (formulated to be 10.5 and 12.5% of DM) with and without LP (11 mg/kg diet DM). Ruminal fluid was collected via stomach tube on d 91 from one steer randomly selected from each pen. No CP x LP interactions were observed with performance data (P > .64). Final weight and total gain were greater (P < .07) for 12.5% CP and LP compared with 10.5% CP and control steers, respectively. Also, DMI was increased (P = .08) with 12.5% CP but not with LP supplementation (P = .36). In addition, ADG and gain:feed ratio were greater (P < .03) for both 12.5% CP and supplemental LP. Ruminal NH3 N concentration was greater (P < .09) with 12.5% CP and LP. Total VFA concentration and molar proportion of acetate were not affected by treatment (P > .11). However, propionate concentration was increased (P < .09) with 12.5% CP and LP, and acetate:propionate was lower (P = .02) with LP supplementation. In Exp. 2, six steers were used in a replicated 3 x 3 Latin square design to compare ruminal fermentation and protein degradation in steers without ionophore feeding or adapted to LP or monensin. In vitro deamination of amino acids by adapted ruminal microbes was also assessed. Ionophore supplementation decreased (P = .07) ruminal NH3 N concentration compared with control steers, and LP increased (P = .02) ruminal NH3 N compared with monensin. Molar proportion of acetate was decreased (P = .02) and propionate increased (P = .01) with ionophore treatment. Consequently, ionophore supplementation depressed the acetate:propionate ratio (P = .01). In situ degradation rate of soybean meal (SBM) CP was greater (P = .09) with ionophore treatment, but estimates of SBM undegradable intake protein were not altered by treatment (P > .25). Microbial specific activity of net NH3 N release and alpha-amino N degradation were decreased (P < .04) with ionophores. Based on this study, LP and monensin did not affect the extent of ruminal degradation of SBM CP but decreased amino acid deamination.  相似文献   

19.
Eight Holstein steers with cannulas in the rumen and proximal duodenum were used in a split-plot design experiment to evaluate the interaction of body weight (175 vs. 370 kg) and level of fat supplementation (0, 3, 6, and 9% yellow grease) on characteristics of digestion and feeding value of fat in finishing diets. Dry matter intake was restricted to 2% of BW. There were no interactions between BW and level of fat supplementation (P > 0.10) on ruminal or total-tract digestion. Level of supplemental fat decreased (linear, P < 0.01) ruminal digestion of OM and NDF, and increased (linear, P < 0.05) ruminal N efficiency. There were no treatment effects (P > 0.10) on postruminal digestion of OM, NDF, and N. There tended to be an interaction (P < 0.10) between BW and level of fat supplementation on postruminal starch digestion. Increasing level of fat supplementation increased postruminal digestion of starch in heavier steers but did not affect starch digestion in lighter steers. There were no interactions (P > 0.10) between BW and level of fat supplementation on postruminal fatty acid digestion. Increasing level of fat supplementation decreased (linear, P < 0.01) postruminal fatty acid digestion, which was due to a decreased (linear, P < 0.01) postruminal digestion of C16:0 and C18:0. Supplemental fat decreased (linear, P < 0.01) total-tract digestion of OM and NDF. The estimated NEm (Mcal/kg) of yellow grease averaged (linear, P < 0.01) 6.02, 5.70, and 5.06 for the 3, 6, and 9% of level supplementation, respectively. We conclude that intestinal fatty acid digestion (FAD, %) is a predictable function (r2 = 0.89; P < 0.01) of total fatty acid intake per unit body weight (FAI, g/kg BW): FAD = 87.560 - 8.591FAI. Depressions in fatty acid digestion with increasing level of intake were due primarily to decreased intestinal absorption of palmitic and stearic acid. Level of fatty acids intake did not appreciably affect intestinal absorption of unsaturated fatty acid. Changes in intestinal fatty acid digestion accounted for most of the variation in the NE value of supplemental fat.  相似文献   

20.
An experiment was carried out at Alemaya University in Ethiopia to investigate the effect of night kraaling on the dry matter intake (DMI), live weight gain (LWG) and foraging behaviour of Ogaden cattle. Three groups of four animals were given either 7 h access to pasture per day, simulating traditional grazing (TG) practice; extended grazing (EG) access for 11 h per day; or traditional grazing access plus a nocturnal forage supplement (TF). Live weight gain, DMI and foraging behaviour were measured during the late dry season (EP1) and the wet season (EP2). None of the treatments had any significant effect on either DMI or LWG during EP1 or EP2. Extending pasture access time from 7 h to 11 h did not significantly increase the amount time spent grazing, but grazing intensity was significantly (p < 0.05) reduced during the non-common grazing hours. Step rate was significantly lower (p < 0.01) during EP2 than during EP1 and bites per step were significantly higher (p < 0.001) during EP2 than EP1, indicating that animals had to travel a shorter distance before selecting material to eat during the wet season (EP2). Providing supplementary forage (TF) had no significant effect on any measured parameter. In this study neither of the two low-cost methods (EG and TF) of improving access to forage had any beneficial effect on cattle productivity. It is concluded that, under the prevailing conditions, the traditional grazing practices of this part of Ethiopia do provide sufficient pasture access time to achieve daily voluntary food intake.  相似文献   

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