<Emphasis Type="Italic">In vitro</Emphasis> chemosensitivity of canine mast cell tumors grades II and III to all-<Emphasis Type="Italic">trans</Emphasis>-retinoic acid (ATRA).     

K. C. Pinello  M. Nagamine  T. C. Silva  P. Matsuzaki  H. V. Caetano  L. N. Torres  H. Fukumasu  J. L. Avanzo  J. M. Matera  M. L. Z. Dagli 《Veterinary research communications》2009,33(6):581-588

Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect the skin and soft tissue of dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. While retinoids are well recognized as promising antitumor agents, there have been only a few reports about retinoids’ effect on canine cancers. The aim of this study was to investigate the chemosensitivity of MCT grades II and III to all-trans retinoic acid (ATRA). Immediately after surgical resection, MCT were prepared for primary culture. Samples of MCTs were also fixed in formalin for histopathology and grading according to the classification of Patnaik et al. (Veterinary Pathology 21(5):469–474, 1984). The best results were obtained when neoplastic mast cells were co-cultivated with fibroblasts. Cultured mast cells were, then, treated with concentrations of 10⁻⁴ to 10⁻⁷ M of ATRA, in order to evaluate their chemosensitivity to this retinoid. MTT assay was performed to estimate cell growth and death. The highest level of mast cell chemosensivity was obtained at the dose of 10⁻⁴ M (p < 0,002). MCT of grades II or III were equally susceptible to the treatment with ATRA. Cell death was observed on the first 24 h until 48 h. According to these results, ATRA may be a potential chemotherapeutic agent for the treatment of canine MCT.  相似文献   

Immune responses to haemorrhagic septicaemia (HS) vaccination in <Emphasis Type="Italic">Trypanosoma evansi</Emphasis> infected buffalo-calves     

Lachhman D. Singla  Prayag D. Juyal  Narinder S. Sharma 《Tropical animal health and production》2010,42(4):589-595

To assess the immunosuppressive effect of Trypanosoma evansi infection in buffalo-calves on immune responses to heterologous antigen, the study was planned to examine the responses of haemorrhagic septicaemia vaccination in simultaneously and previously (80 days before vaccination) T. evansi-infected buffalo-calves. Eight buffalo-calves were divided into three groups. Buffalo-calves of group A (n = 3) were previously (80 days before primary vaccination with haemorrhagic septicaemia [HS] vaccine) infected with T. evansi (1 × 10⁷ tryps.calf⁻¹; sc) and that of group B (n = 3) were infected with T. evansi (1 × 10⁷ tryps.calf⁻¹; sc) on the day of primary vaccination with HS vaccine. Two healthy uninfected control calves given only HS vaccine were kept in group C. All the buffalo-calves were given a booster dose of vaccine 21 days post-primary vaccination (PPV). Twenty eight days PPV, animals of group A were given trypanocidal quinapyramine prosalt at 6.66 mg kg⁻¹. Immunosuppressive effect of T. evansi infection was evident from day 7 PPV with HS vaccine. The effect was more pronounced in previously T. evansi-infected buffalo-calves as compared with simultaneously infected buffalo-calves. Group A buffalo-calves appeared to have recovered from the immunosuppressive effect after 28 days post-trypanocidal treatment as observed by humoral and cell-mediated immune responses. Immunosuppressive effect to HS vaccination was observed in T. evansi-infected buffalo-calves, and trypanocidal therapy enabled the calves to mount the responses similar to uninfected controls.  相似文献   

PCR based detection of furadan genotoxicity effects in rohu (<Emphasis Type="Italic">Labeo rohita</Emphasis>) fingerlings     

G. Mohanty  J. Mohanty  S. K. Garnayak  S. K. Dutta 《Veterinary research communications》2009,33(7):771-780

The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control and treated groups at each of the four sampling hours. A total of six selected RAPD primers were used for PCR amplification. Template stability has been taken as the measure of DNA damage caused by pesticide. The results obtained showed no significant difference in the template stability in the blood DNA of furadan treated groups at any of the four sampling hours; however, the liver DNA were able to show significant difference at 48 and 96 hours of treatment.  相似文献   

Proliferation and transmission patterns of <Emphasis Type="Italic">Pasteurella multocida</Emphasis> B:2 in goats     

Shafarin MS  Zamri-Saad M  Khairani BS  Saharee AA 《Tropical animal health and production》2008,40(5):335-340

This report describes the proliferation and transmission patterns of Pasteurella multocida B:2 among stressful goats, created through dexamethasone injections. Thirty seven clinically healthy adult goats were divided into three groups consisted of 15 goats in group A, 11 goats in group B and the remaining 11 in group C. At the start of the study, all goats of group A were exposed intranasally to 1.97 × 10¹⁰ CFU/ml of live P. multocida B:2. Dexamethasone was immediately administered intramuscularly for 3 consecutive days at a dosage rate of 1 mg/kg. The exposed goats were observed for signs of HS for a period of 1 month. At the end of the 1-month period, 11 goats from group B were introduced into and commingled with the surviving goats of group A before all goats from both groups were immediately injected intramuscularly with dexamethasone for 3 consecutive days. The treatment with dexamethasone was then carried out at monthly interval throughout the 3-month study period. Goats of group C were kept separately as negative control. Three surviving goats from each group were killed at 2-week interval for a complete post-mortem examination. Two (13%) goats of group A were killed within 24 hours after intranasal exposure to P. multocida B:2 while another two (13%) goats from the same group were killed on day 40, approximately 10 days after the second dexamethasone injection. All four goats showed signs and lesions typical of haemorrhagic septicaemia. Bacteraemia was detected in 3 goats of group A that were having rectal temperature higher than 41°C. The P. multocida B:2 isolation pattern was closely associated with dexamethasone injections when significantly (p < 0.05) higher rate of isolations from both groups were observed after each dexamethasone injection. Transmission of P. multocida B:2 from goats of group A to group B was successful when P. multocida B:2 was isolated from goats of group B for a period of 28 days. There was a strong correlation between dexamethasone injections, rate of bacterial isolation and serum cortisol level. The IgG level showed an increasing trend 2 weeks after exposure to P. multocida B:2 and remained high throughout the study period.  相似文献   

Biological control of <Emphasis Type="Italic">Ascaris suum</Emphasis> eggs by <Emphasis Type="Italic">Pochonia chlamydosporia</Emphasis> fungus     

Ferreira SR  de Araújo JV  Braga FR  Araujo JM  Frassy LN  Ferreira AS 《Veterinary research communications》2011,35(8):553-558

Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3 did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately 100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs.  相似文献   

Cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in Cirrhinus mrigala infected with Aeromonas hydrophila     

Sahoo PK  Mohanty BR  Kumari J  Barat A  Sarangi N 《Comparative immunology, microbiology and infectious diseases》2009,32(6):527-537

Transferrin partial complementary DNAs were cloned from the livers of five species in four genera of Indian carps (Indian major carp species: Labeo rohita, Catla catla and Cirrhinus mrigala; medium carp: Puntius sarana; minor carp: Labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cDNA sequences. The partial transferrin cDNAs of the five species of carps had sizes from 624 to 633 bp (487 bp for L. rohita) and encoded an open reading frame consisting of 206–211 (162 for L. rohita) amino acids. The alignments of carp cDNA sequences showed 85–97% homology and 71–93% homology in deduced amino acid sequences. A phylogenetic tree of amino acid sequences of transferrin cDNAs from carps showed that the relationship among the four genera of Indian carps is well correlated with that derived from classic morphologic analyses. The hypothesized cleavage site and interdomain bridge of transferrin molecule were predicted for the above carp species and interestingly the cleavage site amino acid sequence was found to be conserved among all the carps. To study the tissue-specific expression of the transferrin gene, various tissues (liver, kidney, spleen, brain, muscle, testis, heart, intestine, gill and fin) from apparently healthy (control), moribund and survived C. mrigala experimentally infected with Aeromonas hydrophila infection were analyzed. Transferrin mRNA was detected only in liver RNA and to lesser extent in brain tissue out of the 10 tissues analyzed irrespective of bacterial infection.  相似文献   

Ovicidal activity of seven <Emphasis Type="Italic">Pochonia chlamydosporia</Emphasis> fungal isolates on <Emphasis Type="Italic">Ascaris suum</Emphasis> eggs     

Ferreira SR  Araújo JV  Braga FR  Araujo JM  Carvalho RO  Silva AR  Frassy LN  Freitas LG 《Tropical animal health and production》2011,43(3):639-642

The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.  相似文献   

Setting New Immunobiological Parameters in the Hamster Model of Visceral Leishmaniasis for <Emphasis Type="Italic">In Vivo</Emphasis> Testing of Antileishmanial Compounds     

Dea-Ayuela MA  Rama-Iñiguez S  Alunda JM  Bolás-Fernandez F 《Veterinary research communications》2007,31(6):703-717

To establish suitable immunobiological parameters for in vivo testing of new antileishmanial compounds in the golden hamster model of visceral leishmaniasis, two groups of 8 animals were infected each with 10⁵ or 10⁷ stationary promastigotes by the intracardiac route and the clinical and immunoparasitological features were monitored up to day 155 after infection. All animals became infected at both doses, although significant differences were observed between parasite burdens in liver and spleen. The mean number of parasites in animals infected with 10⁷ promastigotes increased by 9.5 times in liver and by 43.1 times in spleen compared with those infected with 10⁵ promastigotes. In animals given the higher dose, the outcome of the disease occurred between days 75 and 90 after infection, whereas no signs of disease were apparent in those given the lower infecting dose. Positive antibody (IgG) responses were detected earlier (week 5–7 after infection) in animals infected with the higher dose than in those infected with the lower dose (week 8–10 after infection), but these responses did not correlate with individual parasitological loads in liver and spleen. An inverse correlation was observed between infecting doses and in vitro spleen lymphocyte proliferation against mitogens (ConA). The proportion of CD4⁺ and CD19⁺ spleen cell increased in animals given the higher infection, whereas it decreased in those given the lower infection compared to naive controls.  相似文献   

Non-enzymatic antioxidant status and modulation of lipid peroxidation in the muscles of <Emphasis Type="Italic">Labeo rohita</Emphasis> by sub lethal exposure of CuSO<Subscript>4</Subscript>     

S. D. Jena  M. Behera  J. Dandapat  N. Mohanty 《Veterinary research communications》2009,33(5):421-429

Xenobiotics-mediated environmental stress is an important determining factor in the maintenance of fish health as fishes are frequently exposed to such components. Increasing evidences indicate that acute and chronic xenobiotic exposure modulates ROS production, suppresses immune response and increase the incidence of fish diseases. In the present context an attempt has been made to study the in vivo effect of different concentrations of CuSO₄ (0.5, 1.00 or 2.00 ppm) on lipid peroxidation (an index of oxidative stress) and non enzymatic antioxidant status (glutathione and Ascorbic acid), in the muscle of a widely consumed freshwater fish Labeo rohita. From the out come of this study it is concluded that comparatively low dose of copper (0.5 ppm) induce mild oxidative stress in the experimental fish with concomitant elevation of GSH and AsA content of the muscle. However, high concentration of CuSO₄ (2.00 ppm) in the ambient water leads to severe oxidative stress manifested in the form of LPX and morphoanatomical alteration.  相似文献   

Haematology and Serum Biochemistry of Chital (<Emphasis Type="Italic">Axis axis</Emphasis>) and Barking Deer (<Emphasis Type="Italic">Muntiacus muntjak</Emphasis>) Reared in Semi-Captivity     

Gupta AR  Patra RC  Saini M  Swarup D 《Veterinary research communications》2007,31(7):801-808

Haematological and serum biochemical values of clinical significance that could serve as reference data for deer kept in captivity were measured for chital (Axis axis) and barking deer (Muntiacus muntjak). The venous blood from four each of chital and barking deer (n = 8) reared in semi-captivity was collected after proper restraint of the animals. The mean blood haemoglobin, packed cell volume, total erythrocyte count and total leukocyte count of all the eight deer of the two species were 15.90 ± 0.44,g/dl, 51.44 ± 0.60%, 20.83 ± 0.57 × 10⁶μl and 2.37 ± 0.20 × 10³μl. Serum total protein, albumin, bilirubin, cholesterol and blood urea nitrogen irrespective of species were 6.83 ± 0.19,g/dl, 3.90 ± 0.11,g/dl, 0.33 ± 0.08,mg/dl, 106.81 ± 3.59,mg/dl and 24.79 ± 2.11,mg/dl, respectively. Serum enzyme activities indicative of liver function such as alanine transaminase (ALT) and aspartate transaminase (AST) were 30.38 ± 4.67,units/ml and 42.88 ± 5.97,units/ml, respectively. The serum calcium and phosphorus levels of all the eight deer were 10.27 ± 0.36,mg/dl and 8.31 ± 0.68,mg/dl, respectively. This is the first report on baseline values in barking deer. The distribution of haematological and serum biochemical values was fairly normal, suggesting that the mean values could be representative of normal values for two different deer species.  相似文献   

Immune responses elicited in mice with recombinant <Emphasis Type="Italic">Lactococcus lactis</Emphasis> expressing F4 fimbrial adhesin FaeG by oral immunization     

Shujie Liu  Yongming Li  Ziwei Xu  Yicheng Wang 《Veterinary research communications》2010,34(6):491-502

Enterotoxigenic Escherichia coli (ETEC) is a major pathogenic agent causing piglet diarrhea. The major subunit and adhesin FaeG of F4⁺ ETEC is an important virulence factor with strong immunogenicity. To determine whether Lactococcus lactis (L. lactis) could effectively deliver FaeG to the mucosal immune system, recombinant L. lactis expressing FaeG was constructed, and immune responses in mice following oral route delivery of recombinant L. lactis were explored. The production of FaeG expressed in L. lactis was up to approximately 10% of soluble whole-cell proteins, and recombinant FaeG (rFaeG) possessed good immunoreactivity by Western blot analysis. Oral immunization with recombinant L. lactis expressing FaeG induced F4-specific mucosal and systemic immune responses in the mice. In addition, high dose recombinant L. lactis or co-administration of high dose recombinant L. lactis with CTB enhanced the immune responses. These results suggested that L. lactis expressing FaeG was a promising candidate vaccine against ETEC.  相似文献   

Comparison of the pathogensis of two isolates of <Emphasis Type="Italic">Besnoitia caprae</Emphasis> in inbred BALB/c mice   总被引：1，自引：1，他引：0  

Ahmad Oryan  Fatemeh Namazi  Mohammad-Mehdi Namavari  Hassan Sharifiyazdi  Marjan Moraveji 《Veterinary research communications》2010,34(5):423-434

This study was performed to evaluate the infectivity of bradyzoites of two Besnoitia caprae isolates, BC-1 and BC-2, to inbred BALB/c mice. Each group of inbred BALB/c mice was inoculated intraperitoneally with 1 × 10³, 1 × 10⁴, 1 × 10⁵, 5 × 10⁵ and 1 × 10⁶ of one of the two isolates of B. caprae bradyzoites. The mice were monitored daily for a period of 40 days for survival. After death of each mice, several passages from its peritoneal washing and tissues were analyzed using ribosomal DNA-specific PCR assay. Marked differences in pathogenicity between the isolates were seen. All the inbred BALB/c mice infected with BC-2 survived but all the mice that were administered with 1 × 0⁵, 5 × 10⁵ and 1 × 10⁶ BC-1 bradyzoites were died within 4–9 days post-infection (DPI). Histopathological examination of the tissues of the dead mice revealed hyperemia and necrosis with presence of mononuclear and polymorphonuclear cell infiltration in myocardium, spleen and intestines together with interstitial pneumonia and peritonitis. All inbred BALB/c mice in the 1 × 10³ and 1 × 10⁴ groups of BC-1 inoculated mice survived and they were euthanized after 40 DPI. Chronic inflammation with infiltration of mononuclear cells was evident in myocardium, spleen, alveolar septa of the lungs of most of the examined tissues with hemorrhagic enteritis in the mice infected with 1x10⁶ bradyzoites. The mice infected with different doses of BC-2 were euthanized after 40 DPI and no lesion was seen in histopathological sections of their organs. All peritoneal washings and examined tissues were PCR positive in BC-1 group. This experiment is the first report to show inbred BALB/c mice as a relevant model for B. caprae and demonstrates that this strain of inbred BALB/c mice is a suitable animal model for biological studies and examination of pathogenesis for this species of Besnoitia. The present findings also provide evidence for significant differences between the two isolates of B. caprae.  相似文献   

Immunopathology of <Emphasis Type="Italic">Dirofilaria immitis</Emphasis> Infection     

Simón F  Kramer LH  Román A  Blasini W  Morchón R  Marcos-Atxutegi C  Grandi G  Genchi C 《Veterinary research communications》2007,31(2):161-171

Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts, with infections occasionally being reported in humans. Studies have shown that both dirofilarial antigens and those derived from its bacterial endosymbiont Wolbachia, interact with the host organism during canine, feline and human infections and participate in the development of the pathology and in the regulation of the host’s immune response. Both innate and acquired immune responses are observed and the development of the acquired response may depend on the host and, or on its parasitological status. This review aims at illustrating current research on the role of both D. immitis and Wolbachia, in the immunology and immunopathology of dirofilariosis.  相似文献   

Reduction of <Emphasis Type="Italic">Theileria annulata</Emphasis> Infection in Ticks Fed on Calves Immunized with Purified Larval Antigens of <Emphasis Type="Italic">Hyalomma anatolicum anatolicum</Emphasis>     

Das G  Ghosh S  Ray DD 《Tropical animal health and production》2005,37(5):345-361

Larval antigen of Hyalomma anatolicum anatolicum, the vector of Theileria annulata, was purified by two-step affinity chromatography using anti-tick gut-specific rabbit IgG and IgG from immunized cattle. The purified antigen showed the presence of a single polypeptide of 37 kDa (GHLAgP) on SDS-PAGE. Two groups (I and II) of naive crossbred calves (Bos taurus × B. indicus) were immunized with 1 mg of GHLAgP in three divided doses. Immunized calves of group I were also infected with a sublethal dose of T. annulata along with a group of non-immunized calves (group III). Animals in groups I, II, III as well a control group (group IV) were challenged with live nymphs of H. a. anatolicum on the 10th day of immunization. There was a significant reduction in the number of emerging adults of 56.9% ± 1.67% in calves of group I (p < 0.01) and 63.09% ± 1.26% in calves of group II (p < 0.001) compared to the controls. The calves of groups I and II showed antibody responses to tick antigen up to day 70 post immunization. Infection with T. annulata was determined in the salivary glands of adult ticks that developed from the nymphs used for challenge infection. In ticks taken from group I calves, there was a 75.0% ± 0.00% infection compared with only 85.0% ± 2.88% infection in ticks taken from calves of group III. Using PCR, a lower infection (83.33% ± 3.33%) was detected in ticks that developed from calves of group I compared with calves from group III (90.00% ± 2.88%). The ground-up tick supernatants (GUTS) of the ticks taken from calves of group III yielded higher infection rate and exhibited higher infectivity titre in in vitro infection assay of bovine mononuclear cells than the GUTS of the ticks taken from calves of group I. The results suggest a partial reduction in growth rate of T. annulata in ticks feeding on calves immunized with GHLAgP.  相似文献   

Immunoprotection of recombinant <Emphasis Type="Italic">Eg</Emphasis>.P29 against <Emphasis Type="Italic">Echinococcus granulosus</Emphasis> in sheep     

Hao Wang  Zihua Li  Fu Gao  Jiaqing Zhao  Mingxing Zhu  Xin He  Nan Niu  Wei Zhao 《Veterinary research communications》2016,40(2):73-79

  相似文献   

The analysis of <Emphasis Type="Italic">groEL</Emphasis> gene in <Emphasis Type="Italic">Salmonella enterica</Emphasis> subspecies <Emphasis Type="Italic">enterica</Emphasis> serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method     

Dilmaghani M  Ahmadi M  Zahraei Salehi T  Talebi A 《Veterinary research communications》2011,35(3):133-143

Salmonella enterica subspecies enterica serovar Typhimurium causes food-borne outbreaks and systemic diseases in humans and animals. groEL gene (also known as mopA gene in S. Typhimurium), possessing conserved sequence, plays an important role in invasion of bacteria. The purpose of present study was to identify the polymorphism of groEL gene among different avians in different regions by PCR-RFLP method. Fifty two S. Typhimurium isolates (Broiler (n = 13), Layer (n = 12), Duck (n = 5), Goose (n = 5), Sparrow (n = 8), Canary (n = 3), Pigeon (n = 5) and Casco parrot (n = 1). were identified using serotyping as well as multiplex-PCR. Then, amplification of groEL gene performed and amplified products subjected to restriction digestion with BsuRI enzyme. Three RFLP profiles, A, B and C, generated DNA fragments between approximately 100–1,000 bp in size, were observed. The RFLP profile A was observed in 35 (67.3%), profile B in 14 (26.9%) and profile C in 3 (5.77%) of isolates. S. Typhimurium isolates recovered from 13 broilers (two of which profile A, 9 profile B and 2 profile C) and from 8 sparrows (two of which profile A, 5 profile B and 1 profile C) showed all three profiles, but 12 layers and other avians (including Canary (n = 3), Goose (n = 5), Duck (n = 5), Pigeon (n = 5) and Casco parrot (n = 1)) showed profile A. None of these profiles was allotted for a special region. The result of present study showed that S. Typhimurium undergoes genetic mutations in groEL gene under unpleasant milieu in different regions and in different avians. Thus, genetic diversity, despite conserved nature of groEL gene in S. Typhimurium, may exist but it depends on the condition where bacteria have settled. To our knowledge, three RFLP profiles of groEL gene generated by BsuRI restriction enzyme were not reported previously.  相似文献   

<Emphasis Type="Italic">Theileria</Emphasis> (<Emphasis Type="Italic">Babesia</Emphasis>) <Emphasis Type="Italic">equi</Emphasis> and <Emphasis Type="Italic">Babesia caballi</Emphasis> Infections in Horses in Galicia,Spain     

Camacho AT  Guitian FJ  Pallas E  Gestal JJ  Olmeda AS  Habela MA  Telford SR  Spielman A 《Tropical animal health and production》2005,37(4):293-302

The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B. caballi. Sixty serum samples (control group) were taken from healthy horses pastured on two farms, and examined for evidence of equine T. equi and B. caballi infection by indirect fluorescent antibody test (IFAT). Of the 60 samples, 24 (40%) and 17 (28.3%) samples were positive for T. equi and B. caballi, respectively. Twelve (20%) samples were positive for both parasites. Haematology and serum biochemistry were compared between controls and a series of 36 horses clinically affected by T. equi (25) or B. caballi (11). Compared with the healthy group, there was a 43% and 37% decrease in the haematocrit for T. equi and B. caballi infection, respectively. Parasitaemic horses presented an intense anaemia and serum biochemistry signs of liver damage. The anaemia was more severe in T. equi-infected than in B. caballi-infected horses. Our results suggest that equine piroplasmosis is widespread in the region and is a cause for concern.  相似文献   

Methicillin and aminoglycoside resistance in <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> isolates from bovine mastitis and sequence analysis of their <Emphasis Type="Italic">mecA</Emphasis> genes     

Hulya Turutoglu  Mustafa Hasoksuz  Dilek Ozturk  Murat Yildirim  Sonay Sagnak 《Veterinary research communications》2009,33(8):945-956

Although methicillin-resistant Staphylococcus aureus (MRSA) were generally isolated from human beings; these agents were recently isolated from various animal species. It has been shown that MRSA isolates are not only resistant to beta-lactam antibiotics, but can also be resistant to the other commonly used antibiotics. In this study, 18 phenotypic methicillin resistant S. aureus isolates from bovine mastitis cases were analyzed by PCR for the presence of mecA gene encoding methicillin resistance and aac(6′)/aph(2″), aph(3′)-IIIa and ant(4′)-Ia genes encoding aminoglycoside resistance. Out of 18 S. aureus isolates (oxacillin MICs, ≥4 μg/ml), 3 were positive for mecA gene. Only one from 3 mecA positive isolates was positive for genes encoding aminoglycoside-modifying enzymes and this isolate carried aac(6′)/aph(2″) in combination with aph(3′)-IIIa gene. The aph(3′)-IIIa gene was detected in 3 isolates. These three isolates carrying the aminoglycoside-modifying enzyme genes were resistant to gentamicin, kanamycin and neomycin. The mecA gene of 3 MRSA isolates was sequenced. All three mecA genes of these isolates were identical to that found in human MRSA strains, except a one-base substitution at nucleotide position 757. From the data presented in this study, it can be concluded that MRSA isolated from bovine mastitis may be originated from human beings, but further studies are needed to investigate the possibility of zoonotic transfer of MRSA.  相似文献   

The effects of replacing <Emphasis Type="Italic">Dichantium</Emphasis> hay with banana (<Emphasis Type="Italic">Musa paradisiaca)</Emphasis> leaves and pseudo-stem on carcass traits of Ovin Martinik sheep     

Carine Marie-Magdeleine  Léticia Liméa  Tatiana Etienne  Cicero H. O. Lallo  Harry Archimède  Gisele Alexandre 《Tropical animal health and production》2009,41(7):1531-1538

A study was done to evaluate banana (Musa paradisiaca) as a forage (leaves and pseudo-stems) for feeding Ovin Martinik lambs (OMK), with the aim to test its impact on carcass quality. Forty four intact OMK male were used after weaning with an initial mean live weight of 14.4 (± 3.3) kg, reared in individual pens. Animals were offered either Dichantium hay (control diet, Dh) or cut chopped leaves and pseudo-stems of banana (experimental diet, Blps). They were fed 200—250 g.d⁻¹ of commercial concentrate. Lambs were slaughtered according to 3 classes of slaughter weight (SW): SW20, SW23 and SW26. Growth and carcass performances of both groups were not significantly different, 77 vs. 81 g.d⁻¹ and 42% vs. 43% hot carcass yield, for Dh vs. Blps, respectively. There was a significant (P < 0.05) decrease (31.0 vs. 29.7%) for the dry matter content of the shoulder for lambs fed the banana diet. However, there was no effect observed for the other chemical component (CP, lipid and mineral 585, 317 and 95 g.kg⁻¹DM, respectively). The shoulder (20% of the carcass whatever the SW) was precocious as demonstrated by the allometry coefficient relative to carcass weight (0.894) significantly (P < 0.01) less than 1. It was concluded that, the use of Blps had no significant effect on growth, carcass weights and yields of the OMK lambs, irrespective of the class of the slaughter weight. From these initial results, the use of banana foliages and pseudo-stems could be recommended as sources of forages.  相似文献   

<Emphasis Type="Italic">Eimeria bovis</Emphasis> meront I-carrying host cells express parasite-specific antigens on their surface membrane     

Ahmed Ibrahem I. Badawy  Kathleen Lutz  Anja Taubert  Horst Zahner  Carlos Hermosilla 《Veterinary research communications》2010,34(2):103-118

Host immune responses conducted against antigens of Eimeria bovis are key factors for the development of protective immunity against this protozoan disease. In this study we investigated the expression of E. bovis-derived antigens on the host cell surface membrane during E. bovis first merogony in vitro. Host cells carrying E. bovis-meront I stages expressed E. bovis host cell surface antigens (EbHCSAg) on their surface membrane which were recognised by hyperimmune sera of calves and by sera from rats immunized with E. bovis merozoites I, when tested by indirect immune fluorescent antibody test (IIFAT), laser scanning confocal microscopy (LSCM) and immune electron microscopy. Expression of EbHCSAg on permissive host cells was earliest detected 7 days p. i., thus coinciding with the onset of the parasite replication. Membrane-associated EbHCSAg were removed from infected host cells by proteinase K, partially by Triton X-100, Triton X-114 and Triton X-405, but not by 1 M NaCl, CHAPS or phospholipase C treatment. Antibodies, affinity-purified on paraformaldehyde/glutardialdehyde (PAGA)-fixed E. bovis meront I-infected bovine host cells bound to the surface meront I-carrying cells and to merozoites I (IIFAT, LSCM) but, in contrast to untreated sera, not to sporozoites. When tested on methanol-fixed merozoites I and sporozoites by IIFAT, affinity-purified antibodies bound to structures in the apical complex area of merozoites I, but not to sporozoites, whilst untreated sera caused diffuse labelling of internal structures of both parasite stages. Immune electron microscopy demonstrated binding of affinity-purified antibodies to micronemes and dense granules of merozoites I. Although the function of EbHCSAg is still unknown, results of this study might suggest an involvement in the development of protective immunity against E. bovis infections.  相似文献