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1.
The Mexican rice borer, Eoreuma loftini (Dyar), is the key pest of sugarcane, Saccharum hybrids, in south Texas, having largely displaced the sugarcane borer, Diatraea saccharalis (F.), and it is moving into rice- and sugarcane-growing areas of east Texas and Louisiana. While a number of alternative weed and crop hosts have been reported, the extent to which they might support Mexican rice borer populations is unknown. This study involved choice assays that compared oviposition preference for and larval infestations of five mature graminaceous weed species. Levels of infestation between sugarcane and corn, Zea mays L., crop hosts and between corn and sorghum, Sorghum bicolor (L.) Moench, were also assessed. We determined that the average number of larval entry holes in sudangrass stems was ≥2.5-fold more than for any of the other four weed host plants, that corn had ≥5.9-fold more larval entry holes than sorghum and ≥8.2-fold more than sugarcane. Greater oviposition and infestation of one non-crop host over another was not related to numbers of stems per plant, but was associated with the greater stem diameter and abundance of dry leaf tissue found in Sudangrass, Sorghum bicolor (L.) Moench ssp. drummondi (Nees ex Steud.) de Wet & Harlan, johnsongrass, S. halepense (L.) and barnyardgrass, Echinochloa crus-galli (L.) P. Beauv.; relative to the other weed species in this study. In terms of the crop plants, stalk diameter and quantity of dry leaf tissue were not associated with numbers of eggs or larval entry holes in the choice assays between corn and sorghum, and between sugarcane and corn. While corn has been known as a host of the Mexican rice borer for at least 84 yr, its role in area-wide population dynamics and control efforts has likely been greatly underestimated.  相似文献   

2.
On-farm field experiments were conducted in 2004 and 2007 to assess the suitability of novaluron, a chitin synthesis inhibitor, for sugarcane borer, Diatraea saccharalis (F.), management in Louisiana sugarcane (Saccharum spp. hybrids). Aerial insecticide applications reproducing commercial production practices were made when D. saccharalis infestation levels exceeded a recommended action threshold. In addition to decreased D. saccharalis infestations, 6.3 – 14.5-fold reductions in end of season injury, expressed as the percentage of bored sugarcane internodes, were observed in plots treated with novaluron. D. saccharalis control in novaluron plots was equivalent to (P > 0.05) or better (P < 0.05) than that achieved with tebufenozide, an ecdysone agonist that has been extensively used for over a decade on sugarcane. With a numerical trend of a 3.1-fold decrease in percent bored internodes, the pyrethroid gamma-cyhalothrin seemed less effective than the biorational insecticides in protecting sugarcane against D. saccharalis. Using continuous pitfall trap sampling, no measurable (P > 0.05) decreases in predaceous and non-predaceous soil-dwelling non-target arthropods were associated with insecticides. However, numerical trends for decreases in immature crickets associated with novaluron and gamma-cyhalothrin were recorded in 2007. Our data suggest that novaluron will fit well in Louisiana sugarcane integrated pest management.  相似文献   

3.
Two sugarcane, Saccharum hybrids, varieties were grown in the greenhouse under well watered or drought conditions to examine the influences of stress on Mexican rice borer, Eoreuma loftini (Dyar), oviposition preference and selected nutritional components without impinging factors common to field conditions that alter responses. Our research revealed that, under controlled conditions, drought induced a wider range and greater uniformity of free amino acid (FAA) accumulations than have been previously reported. Drought stress resulted in increased dry leaf tissue and elevated concentrations of 7 of 9 detectable free essential (for insect health) amino acids in stalks, the chief food of Mexican rice borer larvae. Stressed sugarcane was preferred for oviposition, likely related to greater numbers of dry leaves and heightened host plant nutritional quality. Dry leaf tissue, which is not consumed, may be a cue for improving chances of larvae encountering nutrient-enhanced living tissue, and for concealing eggs in folds. Excised dry leaf tissue from the treatments was indistinguishable for oviposition preference; therefore, biochemical status of living tissue may provide oviposition cues. Varieties exhibited no major genotypic differences in FAA accumulations or oviposition preference.  相似文献   

4.
The Mexican rice borer, Eoreuma loftini (Dyar), attacks crops including corn, Zea mays L., rice, Oryza sativa L., sorghum, Sorghum bicolor (L.) Moench, and sugarcane, Saccharum spp. Strongly resistant varieties of any kind, native or otherwise, have not been identified. A field plot corn variety test using two transgenic Bt varieties, Pioneer 31G71, expressing the Cry1F insecticidal protein, and Golden Acres 28V81, expressing the Cry1A.105, Cry2Ab2, and Cry3Bb1 insecticidal proteins, and two non-Bt controls, Dekalb DKC 69-72 and BH Genetics 9050, all four commonly grown in the Lower Rio Grande Valley of Texas, showed that, although oviposition preference was not affected, 28V81 resisted larval stalk boring to the extent that Mexican rice borer injury was almost non-existent. Pioneer 31G71 was infested nearly as much as the controls, but larval development to adulthood was reduced by ≈70%. Rearing larvae on 5, 50, 500, and 5000 μg of corn leaf tissue per ml of artificial diet showed that, while the three lowest concentrations did not affect larval growth and development, the high concentration of 28V81 reduced survivorship to the pupal stage, decreased weight of 4-wk-old larvae, and prolonged development to pupation. Lower numbers of pheromone trap-captured adults at the edges of commercial Bt and non-Bt corn fields showed that populations were lower at the Bt cornfields, suggesting a lesser rate of adult production. Because corn is a preferred host plant over sugarcane, sorghum or rice, use of resistant transgenic Bt corn varieties will likely protect the crop from the substantial injury that can be caused by the pest. This study also suggests that Bt genes might result in similarly strong resistance when inserted in other vulnerable crops such as sugarcane.  相似文献   

5.
The Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), is a serious pest of sugarcane (Saccharum spp.), sorghum (Sorghum bicolor (L.) Moench), corn (Zea mays L.), rice (Oryza sativa L.), and related graminaceous bioenergy crops. A two-year field study was conducted in Jefferson County, TX to examine the impact of nitrogen (N) fertilization on E. loftini infestations and subsequent yields in cultivars of high-biomass and sweet sorghum. In 2013, percentage of bored internodes and number of adult emergence holes per stalk increased with higher N rates; however, only the percentage of bored internodes was impacted by N in 2014. Yields from both years indicated that N rate was positively associated with increases in stalk weight and ethanol productivity, but not sucrose concentration. Because higher N rates were associated with increased yields despite having greater levels of E. loftini injury, our data suggest that increases in yield from additional N outweigh decreases from additional E. loftini injury. Fertilization rates maintained between the recommended 45 and 90 kg N/ha minimize risks of negative area-wide impacts from increased production of E. loftini adults, while still allowing for optimum yields.  相似文献   

6.
《Field Crops Research》2003,82(1):59-73
Stalk water content is an important variable for a sugarcane simulation model as sugar industries in many countries use cane yield and sucrose content on a wet mass basis for payment and yield reports. The prime objective was to develop a stalk water content module (SWCM) that can be incorporated into a sugarcane simulation model. SWCM starts from consideration of the dynamics of water concentration (ρ, g water g−1 dry matter) along stalks and through seasons. The quantities of stalk water were modelled separately for the top and basal sections of the millable stalks. Field observations showed that the stalk water concentration (ρ) declined from 7.8 to 11.8 in the top internodes to 1.6–2.9 g water g−1 dry matter in bottom internodes. In the basal section, ρ ranged from 1.98 in winter to 2.83 g water g−1 stalk dry matter in summer. A two-parameter equation was used to model ρ and resulted in a range of coefficients of determination from 0.8 to 0.97 for six varieties. The SWCM was developed to simulate both the effects of seasonal variation and the age of internodes on the quantity of stalk water. The module was incorporated into a process oriented model of sugarcane growth for validation against field observations in tropical and subtropical areas of Australia and Hawaii, USA. Comparison of observed yields with cane yield simulated by the model that included the SWCM, gave an average of R2 of 0.95, compared with the average of R2 of 0.97 for simulation of stalk dry matter. The average relative root mean squared error (RMSE) was 15.2% in simulation of cane yield and 15.1% for simulation of cane dry weight. The module can be readily incorporated into a model that simulates sugarcane dry matter so that commercial crop yield can be estimated.  相似文献   

7.
Few studies have focused on the effect that silicon fertilisation has on the incidence of brown rust in sugarcane, even though this crop is Si-accumulating and Si addition has been shown to have positive effects in other plant-pathosystems. The objective of this study was to quantify brown rust development in sugarcane after the addition of silicon fertilizer to texturally distinct soils over three sugarcane cropping cycles. One experiment was conducted for three years with sugarcane grown in 100 L pots receiving four levels of silicon fertilizer (0, 185, 370, 555 kg ha−1 Si), with three different soil types (Quartzipsamment-RQ; Rhodic Hapludox-LV; Rhodic Acrudox-LVdf), in randomised blocks design with four replications. Silicate fertilisation increased Si concentrations in the soil and sugarcane leaves. The incidence of brown rust was influenced by Si rates and soils type for the plant cane and second ratoon. For the plant cane, Si application reduced the maximum rust incidence, which was estimated by the b1 parameter of the monomolecular model, with values reaching 29%, 41% and 47% for the RQ, LV and LVdf, respectively. For the RQ, the b1 value was reduced by 20%, 20% and 59% of the control treatment values for the 185, 370 and 555 kg ha−1 Si application rates, respectively. There was a significant reduction in brown rust incidence as a function of increasing leaf Si concentrations in all soil types. These results suggest that Si fertilisation could play an important role in the management of brown rust for sugarcane.  相似文献   

8.
Four neem-based insecticides, Neemix® (0.25% EC @ 20 mg azadirachtin/liter), Ecozin® (3% EC @ 20 mg azadirachtin/liter), Agroneem® (0.15% EC @ 4.8 mg azadirachtin/liter) and Neem oil (0.25% EC azadirachtin @ 20 mg azadirachtin/liter) and a non-commercial neem leaf powder, were evaluated for oviposition deterrence, antifeedant effect to larvae and toxicity to eggs and larvae of Pieris brassicae (Linn.) on cabbage leaves in the laboratory. The concentrations tested were with in the ranges of recommended field rates. Oviposition deterrence in no-choice, two-choice and six-choice assays, was observed for all the treatments. They exhibited significant (P < 0.01) oviposition deterrence on P. brassicae when compared with a non-treated control. Cabbage leaves treated with the neem-based insecticides were used as an egg-laying substrate. Numbers of eggs oviposited by P. brassicae adults on treated cabbage leaves were significantly lower then those treated with water, but no significant differences were detected among the neem insecticides. They also deterred feeding by Pieris larvae and exhibited significant antifeedant effects. Larvae of P. brassicae on treated leaves stopped feeding and dropped from the leaf, resulting in no or minimal damage. Direct contact with neem-based insecticides decreased the survival of eggs. Survival of larvae fed for 9 days on leaves treated with neem-based insecticides was reduced to 51%, 49%, 48%, 24% and 18% in the Neem oil, Neemix, Agroneem, Ecozin and neem leaf powder treatments, respectively. Therefore, it can be concluded from the present investigations that neem-based insecticides had oviposition deterrence, antifeedant and toxic effect to P. brassicae.  相似文献   

9.
Epuraea domina Reitter (Coleoptera: Nitidulidae) is a flower-visiting insect and a pest that causes corky scar on citrus fruit. This study was conducted to investigate the life cycle of E. domina in a citrus grove. E. domina showed the characteristics of cold breeders as they complete key life history events of oviposition and immature development during the winter. E. domina adults spent the summer in uncultivated habits started to lay eggs in soil near fallen citrus fruit in orchards during late autumn. Oviposition activity continued throughout the winter with a large decrease in activity when the minimum air temperature was <0 °C. Hatched larvae fed on decaying citrus and pupated in soil in the spring. The pupae emerged as new adults in mid-May to feed on the pollen and nectar of citrus flowers, probably followed by their migration to uncultivated habitats in the summer.  相似文献   

10.
Mechoris ursulus (Roelofs) (Coleoptera: Attelabidae) is a pest weevil of Fagaceae oak trees in eastern Asia. The female has a distinct branch-cutting behavior in conjunction with oviposition in the acorns of its host plant. This study analyzed the factors influencing oviposition preference by carrying out continuous field surveys over the course of 2009–2010 and through laboratory rearing. The field survey showed that for both of the hosts, Cyclobalanopsis glaucoides Schottky (Fagales: Fagaceae) and Quercus franchetii Skan, M. ursulus preferred branches with fewer acorns and larger acorns on the same branch for oviposition. Laboratory rearing experiments showed offspring performance (i.e., survival rate and fresh weight of larvae) was significantly and positively correlated with acorn size. Preference for larger acorns could maximize the fitness of offspring by providing sufficient food source and space.  相似文献   

11.
Eleusine indica L. Gaertn. and Paspalum distichum L. are annual and perennial grasses, respectively that are widely distributed in turf and perennial cropping systems throughout Spain. Often, glyphosate is used between rows of perennial crops for control of these grasses, but variable responses have been observed. Sensitivity to glyphosate in each species was examined under greenhouse, laboratory and field conditions. In vitro tests on whole plants of both P. distichum and E. indica revealed no differences in sensitivity to glyphosate for areas with long-term use (treated; T) and no history of use (not treated; NT). The NT population of P. distichum (ED50 73.1 g ae ha−1) was 11.6% more sensitive to glyphosate than NT E. indica (ED50 81.6 g ae ha−1). No differences between T and NT populations of both species were observed for foliar retention of glyphosate as well as accumulation of shikimate. However, glyphosate retention and shikimate accumulation were up to 64 and 24.4% greater, respectively in P. distichum compared to E. indica. Within 96 h after treatment (HAT), foliar absorption of 14C-glyphosate was similar among T and NT populations, but 8.8% higher for P. distichum compared to E. indica. Retention of 14C-glyphosate in treated leaves of P. distichum was approximately 55% lower compared to E. indica. Translocation from the treated leaf into other shoot tissue (2.8-fold) and roots (8.5-fold) was higher for P. distichum versus E. indica. This would suggest that differences in E. indica versus P. distichum response to glyphosate are based upon differential retention in treated leaves and reduced movement out of treated tissue in other shoot and root tissue. In separate field experiments in citrus orchards, glyphosate and other herbicides were applied to assess control of E. indica and P. distichum over two years. Flazasulfuron and cycloxidim resulted in 90% or greater control of both species by 60 days after treatment (DAT). Only glufosinate, oxyfluorfen, paraquat and iodosulfuron resulted in >85% control of E. indica. These corresponding treatments ranged in effectiveness from 73 to 92% on P. distichum. Integration of effective herbicides with modes of action different than glyphosate should be used for management of E. indica and P. distichum and may delay the selection for resistance to glyphosate.  相似文献   

12.
The orange wheat blossom midge, S. mosellana (Gehin) (Diptera: Cecidomyiidae), a global pest of wheat (Tritium aestivum L.), has recently invaded Montana's Golden Triangle, an important wheat producing region. Unchecked, S. mosellana populations can quickly grow to damaging levels. In this study, we document the prevalence of S. mosellana and its main natural enemy, Macroglenes penetrans (Hymenoptera: Pteromalidae), in this newly invaded area of Montana, and compared the effects of irrigated versus dryland cropping systems on S. mosellana and its parasitoid. Different approaches to monitoring S. mosellana populations were examined, including pheromone traps for adults, collection of larvae from wheat heads, and overwinter sampling of soil to measure the levels of larvae and cocoons. Adults of the M. penetrans were surveyed through daily sweep net sampling. This study demonstrated that the emergence of M. penetrans was usually well synchronized with emergence of S. mosellana as the emergence of parasitoids occurred shortly after the pest with the highest peak (90%) at 26 June and 14 July, 2015, respectively. Irrespective of sampling techniques; pheromone traps or soil sampling, no significant difference was observed in population of S. mosellana between irrigated and dryland cropping systems. In contrary, cropping systems played a significant role in the abundance of parasitoids since the irrigated fields had significantly higher population of parasitoids (five times on an average) compared to dryland cropping system. The possible implications of these findings in monitoring of S. mosellane and the establishment of M. penetrans are discussed.  相似文献   

13.
《Field Crops Research》2005,91(2-3):207-215
Sugarcane (Saccharum spp.) is rather unusual among field crops in that it is not the seed that is of economic value, but rather the stalk. Sucrose is extracted from the large stalks that are produced by sugarcane plants. Considering their economic value, it is rather surprisingly that there is very little information concerning the development and size of the individual internodes over the length of sugarcane stalks. The objective of this study was to document internode volume over the sugarcane stalk of four cultivars and to observe changes in individual internode volume later in the growing season and in response to lodging. When harvested in August, the size of the internodes varied in a continuous manner along the stalk with the largest internode occurring at the bottom of the plant at approximately internode #15. One cultivar (CP80-1743) had much less of a decrease in internode volume below internode #15 than the other cultivars, indicating that growth was more concentrated in the lower internodes of this cultivar. Since the internode volume diminished as the apex was approached, much of the harvested material was concentrated in the bottom nodes. Comparison of a mid-season harvest (August) and a final harvest (January) indicated both that additional internodes were added to the stalk and that the size of all internodes increased. That is, plant growth was distributed all along the stalk and not focused on one particular zone. Similarly, the negative impact of plant lodging was uniformly distributed over the entire stalk, although there appeared to be differences in cultivars in the extent to which the growth of the stalk was decreased.  相似文献   

14.
The southern United States has a long growing period between corn, Zea mays L., harvest and first winter frost, so volunteer corn which germinates after harvest has a growing period sufficient for corn earworm, Helicoverpa zea (Boddie) and fall armyworm, Spodoptera frugiperda (J. E. Smith) to feed on these plants. However, lower air temperatures can limit larval development on late season volunteer corn and thereby successful pupation. Here we explore the suitability of late season volunteer corn for larval development and the potential contribution of H. zea larvae to the overwintering population. Our survey revealed the occurrence of volunteer corn in high densities, with monthly mean densities ranging from 56,000 to 143,000 plants ha−1. H. zea larvae were found feeding on both vegetative and reproductive stage plants while S. frugiperda were only found on vegetative stage plants. An analysis of H. zea growing degree day (GDD) accumulations based on Mississippi weather data from 1980 to 2010 revealed that sufficient GDD to reach prepupation would always be accumulated before first frost if oviposition occurred by 9 September, with the probability of successful pupation decreasing rapidly thereafter. However, most of the H. zea larvae were oviposited after this, and could not reach pupation. Because S. frugiperda cannot overwinter in Mississippi, their ability to pupate was not examined. Low suitability of whorl stage corn for H. zea development coupled with low larval densities during this stage effectively diminish the number of larvae that complete development on late season volunteer transgenic corn expressing genes from the soil bacterium, Bacillus thuringiensis (Bt). This limits the Bt resistance risk posed by larvae developing on late season volunteer corn in all but the most southern locations in the US.  相似文献   

15.
Bactericera cockerelli has recently become a major concern because of its direct feeding and vectoring of bacterial diseases in many solanaceous crops. The repellency of four biorational insecticides, MOI-201 (a Chinese medicine plant extract), Requiem (a plant extract of Chenopodium ambrosioides), BugOil (a mixture of four plant essential oils), and SunSpray oil (a mineral oil), to B. cockerelli adults was tested on tomato. In a no-choice test, all the insecticides had significant repellency to adults and deterred oviposition as compared with untreated controls. Of the four insecticides, the two oils showed a stronger repellency to adults and deterred oviposition more strongly than Requiem or MOI-201. In a choice test, all insecticides had significant repellency to adults and deterred oviposition compared to untreated controls. Of the four tested insecticides, <1 adults and no eggs were found on the leaves treated with SunSpray Oil, BugOil or Requiem 3 d after treatment. The repellency rates of these three insecticides were 77.2–95.4%. MOI-201 also repelled adults significantly and deterred oviposition compared to untreated controls even though it was the least effective insecticide among the four evaluated. In conclusion, all four insecticides tested showed significant repellency to B. cockerelli adults and deterred oviposition, especially the two oils. The overall repellency to potato psyllid adults can be arranged in a descending order of SunSpray oil > BugOil > Requiem > MOI-201. These insecticides could be used in integrated pest management programs targeted against the potato psyllid on solanaceous crops.  相似文献   

16.
The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a major target of transgenic corn, Zea mays L., expressing Bacillus thuringiensis (Bt) proteins in both North and South America. A highly Cry1F-resistant strain of S. frugiperda was established from a field collection in Puerto Rico in 2011. In this study, three greenhouse trials were conducted to evaluate larval survival and leaf injury of Cry1F-susceptible, -resistant, and -heterozygous genotypes of S. frugiperda on whole plants of five non-Bt and eight Bt corn hybrids. The Bt corn products included two single-gene Bt corn hybrids containing Herculex®I (Cry1F) and YieldGard® (Cry1Ab) traits and six pyramided Bt corn hybrids representing four traits: Genuity® VT Double Pro™, Genuity®VT Triple Pro™, Genuity® SmartStax™, and Agrisure® Viptera™ 3111. In each trial, neonates of S. frugiperda were placed into the plant whorls at vegetative plant stages (V6–V10). Larvae of the three insect genotypes on non-Bt corn hybrids survived well and caused serious plant injury. Cry1Ab corn was ineffective against all three insect genotypes. On Cry1F corn plants, resistant larvae survived on 72.9% plants after 12–15 d and caused a leaf injury rating (Davis' 1 to 9 scales) of 5.7 after 7 d and 7.6 after 12–15 d. Both the larval survivorship and leaf injury rates of the resistant larvae on Cry1F corn plants were not significantly different from those observed on non-Bt corn hybrids. In contrast, no live larvae and little or no leaf injury were observed on the Cry1F corn plants that were infested with susceptible or heterozygous genotypes, or on the pyramided Bt plants. The results demonstrated that the Cry1F-resistant S. frugiperda was highly resistant to whole plants of Cry1F corn and the resistance was recessive. Hybrids that contained one of the four pyramided Bt traits were effective for managing the Cry1F resistance in S. frugiperda.  相似文献   

17.
《Field Crops Research》2002,77(1):43-49
Influence of application of phosphorus solubilizing bacteria (PSB), Bacillus megatherium var. Phosphaticum, at 10 kg ha−1 of lignite based culture with and without varying amounts of P fertilizer was studied on soil available P changes and sugarcane growth and yield. The PSB application increased the PSB population in the rhizosphere and the plant available P status in the soil. It also enhanced tillering, stalk population and stalk weight, and led to a cane yield increase of 12.6% over no application. When used in conjunction with P fertilizers, PSB reduced the required P dosage by 25%. In addition, it was found that 50% of the costly super phosphate could be replaced by rock phosphate (RP), a cheap source of P, when applied in conjunction with PSB. The PSB improved juice quality and sugar yields. The influence of PSB was greatest when RP constituted a part of the P fertilizers applied.  相似文献   

18.
The fungal pathogen Sclerotium rolfsii isolate SC64 is being assessed as the basis for a mycoherbicide for biological control of broadleaf weeds in dry-seeded rice fields. Species tested for susceptibility in the field included Cyperus difformis, Lindernia procumbens, Rotala rotundifolia, Ammannia baccifera and Eclipta prostrata. Following preliminary small plot field applications in summer 2008 and 2010, applications of fungus-infested solid substrates (mixture of rice hulls and bran) of 60-140 g m−2 were conducted at two sites, Nantong and Rugao, in Jiangsu province, China in summer 2010. The sites included a one-year fallow field and a wheat-rice rotation field. Plant mortality was recorded 7 and 14 days after inoculation (DAI). Percentage mortality ranged from 50 to 89% and 30-71% in the 2008 and 2010 solarium small plot trials, respectively. At the Nantong site field trial, 30-60% plant mortality and 31-59% fresh weight reduction were recorded at 14 DAI when applied for the first time but the efficacy increased to 39-86% and 42-90% for plant mortality and fresh weight reduction at 14 DAI with a repeated application. Higher levels of plant mortality (42-77%) and fresh weight reduction (52-82%) were achieved at 14 DAI at the Rugao site with a single treatment, due to the lower weed density and more favourable temperature and humidity conditions at the time of pathogen application. Results confirmed that S. rolfsii SC64 is a potential biocontrol agent of some of the broadleaf weeds tested in dry-seeded rice.  相似文献   

19.
The internal colonization of Brassica napus plants by Metarhizium anisopliae was demonstrated and the endophytic and direct effects of the fungus against Plutella xylostella larvae were assessed. Internal colonization of the plant by the fungus was shown by re-isolating the fungus from leaves, petioles and stems of the plant that were not the initial site of inoculation. Results indicated that significant differences (at P = 0.05) were obtained in mean % successful re-isolation of the fungus from leaves, petioles and stems at 2, 3 and 4 weeks after inoculation. To assess the endophytic effect of the fungus, larvae of P. xylostella were introduced onto plant leaves distant from the initial site of inoculation with the fungus (one leaf per plant). Results indicated that significant differences (at P = 0.05) were obtained in mean % mortality of larvae that were introduced at 2, 3 and 4 weeks after inoculation compared to the non-inoculated control. Similar significant results were obtained when the direct effect of the fungus against the larvae was assessed. In conclusion, this research provides evidence for the first time of the endophytic action of M. anisopliae against P. xylostella larvae and the ability of the fungus to colonize the internal tissues of B. napus plants.  相似文献   

20.
Replicated field trials were conducted to determine the effect of 1,3-dichloropropene (1,3-D) as a potential alternative for methyl bromide (MeBr) in tomato–cucumber rotations in two successive cropping seasons in China. Fumigation with MeBr (400 kg ha−1), three 1,3-D doses (180, 120 and 90 l ha−1), an avermectin dose (187.5 g ha−1) and an untreated control were compared. Tomato data revealed that MeBr was generally superior to the treatments involving 1,3-D and avermectin, which in turn were superior to the control, for improving tomato crop yield and inhibiting Meloidogyne incognita, weeds and mortality caused by plant disease. In a successive cucumber crop, all fumigants tested except avermectin, showed significant continual influence in the same plots. In most cases, the highest 1,3-D dose was comparable to MeBr. Overall, in both growth seasons, 1,3-D at the dose of 180 l ha−1 was as effective as MeBr in increasing plant height, yield and in reducing the incidence of soil borne disease, especially in maintaining excellent M. incognita control, but it provided only moderate control of weeds. On the basis of these results, combining 1,3-D with other alternatives to MeBr, is recommended for satisfactory control of soil pests in tomato–cucumber rotations in China.  相似文献   

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