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银黄注射液对机体免疫功能的影响 总被引：1，自引：0，他引：1
银黄注射液主要由黄芩、金银花、连翘等多味中药组成,其单药分别的杀菌作用已得到证实,黄芩活血通经、散结消肿,能影响体细胞的代谢,增强细胞的免疫功能,提高淋巴细胞的杀菌能力,药理实验表明,黄芩具有广谱抗菌作用,能促进淋巴细胞生成,增加机体免疫力。金银花清热解毒、凉散风热。连翘解热镇痛、降低毛细血管的通透性。诸药合用,具有清热解毒、抗菌消炎、通经活血、消肿止痛、活络通乳作用。资料显示,银黄注射液既能通过抗菌消炎来达到防治乳房炎的作用,同时也能调节机体的免疫作用,达到标本兼治的效果。为探讨银黄注射液治疗机理,本试验通过建立兔乳房炎模型,并通过血液白细胞计数、脾淋巴细胞转化增殖试验和巨噬细胞吞噬功能试验来考察银黄注射液对乳房炎免疫功能的影响。结果表明,模型组白细胞总数升高,与正常对照组比较有显著的差异,用药后,青链霉素组和银黄注射液组白细胞总数下降,与模型组比较有显著的差异;与正常对照组比较,模型组的脾淋巴细胞增殖转化能力显著下降（P＜0.05）,与模型组比较,银黄注射液组和青链霉素组的脾淋巴细胞增殖转化能力显著升高（P＜＞0.05）;同时在考察银黄注射液对小鼠腹腔巨噬细胞吞噬试验可以看出,注射银黄注射液后,小鼠腹腔巨噬细胞吞噬功能明显增强,与黄芪多糖注射液组比较,无显著差异（P＞0．05）。 相似文献
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《黑龙江畜牧兽医》2017,(22)
为了研究不同剂量的葎草乙醇提取物对小鼠血液淋巴细胞转化率和腹腔巨噬细胞吞噬活性的影响,试验将48只健康昆明小鼠随机分为对照组(灌胃生理盐水),葎草提取物低剂量(100 mg/kg)组、中剂量(200 mg/kg)组及高剂量(300 mg/kg)组,每组3个重复,每个重复4只,低、中、高剂量组小鼠每天分别按体重灌胃葎草提取物100,200,300 mg/kg,每天1次,连续灌胃30 d。肌肉注射植物血细胞凝集素用以检测血液淋巴细胞转化率;腹腔注射鸡红细胞,取腹腔液,检测巨噬细胞吞噬百分率和吞噬指数。结果表明:葎草提取物中剂量组小鼠的淋巴细胞转化率和巨噬细胞吞噬百分率、吞噬指数均极显著高于低剂量组和高剂量组(P0.01)。说明葎草提取物能提高血液淋巴细胞转化率和巨噬细胞吞噬活性,提高机体免疫功能。 相似文献
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复方白毛藤注射剂对小鼠免疫调节作用的试验研究 总被引：1，自引：0，他引：1
复方白毛藤注射液是经水醇法制备而成,以小鼠为试验动物模型,连续给药15d后,通过ConA诱导的小鼠脾淋巴细胞转化试验小鼠碳粒廓清试验、二硝基氟苯(DNFB)诱导迟发型变态反应(DTH)试验及腹腔巨噬细胞吞噬功能试验测定脾淋巴细胞增殖能力、DNFB诱发的DTH反应的强度、腹腔吞噬细胞的吞噬率及廓清指数。结果表明。高剂量组显著增强ConA诱导的脾淋巴细胞增殖能力和小白鼠对DNFB诱发的DTH反应;同时吞噬百分数、吞噬指数、廓清指数也明显高于对照组。表明复方白毛藤注射剂具有免疫增强的调节作用。 相似文献
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复方白毛藤注射剂对小鼠的免疫调节作用 总被引：1，自引：0，他引：1
为研究复方白毛藤注射剂对小鼠免疫调节作用 ,采用常规免疫学方法进行了 con A诱导的小鼠淋巴细胞转化增殖试验 ,硝基氟苯诱导迟发型变态反应试验 ,腹腔巨噬细胞吞噬功能试验及小鼠碳粒廓清试验。结果表明 :该制剂中 ,高剂量组均具有明显增强 con A诱导的小鼠脾淋巴细胞增殖能力 ,能显著增强硝基氟苯诱导的小鼠迟发型变态反应 ,明显增强小鼠腹腔巨噬细胞吞噬鸡红细胞能力及小鼠碳粒廓清能力。与对照组相比差异极显著 (P <0 .0 1 ) ,中、高剂量组差异不显著 (P >0 .0 5)。 相似文献
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氯化钐,氯化镨对小鼠免疫功能的影响 总被引：18，自引：0，他引：18
以小鼠为对象，应用实验形态学与免疫学方法，研究了氯化钐（ＳｍＣｌ３）、氯化镨（ＰｒＣｌ３）对经用人Ａ型红细胞免疫后，其特异性抗体（简称抗Ａ抗体）形成、淋巴细胞转化及腹腔巨噬细胞吞噬功能的影响。实验结果表明，ＳｍＣｌ３，及ＰｒＣｌ３剂量为５、０．５及０．０５ｍｇ／ｋｇ时，对小鼠抗Ａ抗体形成、淋巴细胞转化及腹腔巨噬细胞吞噬功能均具有明显的促进作用（Ｐ＜０．０１），尤以小剂量组（０．０５ｍｇ／ｋｇ）的促进作用更为显著。 相似文献
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本实验用黄连解毒汤和加减消毒饮注射液（分别制成）经小鼠腹腔注射，观察对腹腔巨噬细胞吞噬功能的影响，结果表明，上述方剂注射液对小鼠巨噬细胞吞噬功能均有明显的促进作用（P＜0．01）。黄连解汤注射液的效果优于加减消毒饮。此外，氢化可的松对小鼠腹腔巨噬细胞吞噬功能有明显的抑制作用，注射氢化可的松的同时给予加减消毒饮，则能对抗氢化可的松的抑制作用，使巨噬细胞吞噬和蚕噬指数维持在正常水平。 相似文献
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为研究复方天门冬多糖注射液对小鼠免疫功能的影响,采用环磷酰胺(CTX)作为抑制剂建立小鼠免疫抑制模型,注射不同配比的复方天门冬多糖注射液,测定小鼠胸腺和脾脏脏器指数、血清白细胞介素2(IL-2)水平及腹腔巨噬细胞的吞噬百分率和吞噬指数,并从组织形态上观察胸腺与脾脏的结构变化。结果表明,复方天门冬多糖注射液对小鼠胸腺指数和脾脏指数无显著影响;复方天门冬多糖Ⅳ组可以极显著提高小鼠血清中IL-2的水平(P<0.01);复方天门冬多糖Ⅰ组、Ⅳ组极显著提高小鼠腹腔巨噬细胞的吞噬能力(P<0.01);复方天门冬多糖能不同程度地减缓环磷酰胺所致小鼠胸腺和脾脏的萎缩,增加胸腺皮质细胞数量,保持脾小结完整和增加脾淋巴细胞数量。表明复方天门冬多糖对小鼠的非特异性免疫功能具有一定的促进作用。 相似文献
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T M Jacks F R Judith S D Feighner R O Likoff 《American journal of veterinary research》1986,47(11):2325-2328
The 21 field isolates of Treponema hyodysenteriae which were tested were sensitive to 3-acetyl-4'-isovaleryl tylosin (AIV); the minimal inhibitory concentration was 0.25 to 16 micrograms/ml. 3-Acetyl-4'-isovaleryl tylosin administered prophylactically to pigs at concentrations of 5 to 100 mg/kg of feed and tylosin at 110 mg/kg of feed for 28 or 31 days prevented swine dysentery induced by tylosin-sensitive T hyodysenteriae strain SQ2; 15 nonmedicated, inoculated control pigs had bloody diarrhea, and 9 pigs died. In 2 additional trials, AIV administered prophylactically for 28 days at 55 or 110 mg/kg of feed prevented swine dysentery induced by tylosin-insensitive T hyodysenteriae strain B204. All of the inoculated principal pigs medicated with AIV at 55 or 110 mg/kg of feed or carbadox at 55 mg/kg of feed and the noninoculated sentinel pigs for each group had solid feces throughout the 56-day trial. In the nonmedicated, inoculated control groups, bloody diarrhea began at 4 to 5 days after inoculation was done, and 9 of 10 principal pigs and 6 of 9 sentinel pigs had dysentery; 2 pigs died. In the groups medicated with AIV at 27.5 or 5.5 mg/kg of feed, all 5 principal pigs and 3 or 4 sentinel pigs in each group had dysentery; 3 or 4 pigs in each group died. In the group medicated with tylosin at 110 mg/kg of feed, 7 of 10 principal pigs and all 9 sentinel pigs had dysentery; 1 pig died.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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苷肽注射液对犬淋巴细胞转化试验 总被引：3，自引：3，他引：0
以20只健康犬为研究对象,随机分为四组,每组5只,分别注射苷肽注射液、苷肽注射液和犬五联疫苗、犬五联疫苗、生理盐水,研究苷肽注射液及其与疫苗配合进行的对犬淋巴细胞转化试验。各组犬于注射的第1、2、7、14、21天采血,进行淋巴细胞转化试验。结果表明:苷肽注射液单独应用和与疫苗同时应用,在注射的第2天就可以显著提高淋巴细胞转化率(P0.05),且能维持14～21天;疫苗组在注射的第7天达到了最高值,但明显低于前两组。这说明了苷肽注射液具有增强免疫的作用,并且可延长疫苗维持较高免疫功能的时间。 相似文献
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Bock-Gie Jung Jin-A Lee Bong-Joo Lee 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(2):135-141
Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model, dietary supplementation with GB enhanced concanavalin A (ConA)-induced lymphocyte proliferation and increased the percentage of CD3+CD8+ T lymphocytes. In pigs experimentally infected with PRRSV, viral titers in lungs and lymphoid tissues from the GB-fed group were significantly decreased compared to those of the control group 12 days post-infection. Corresponding histopathological analyses demonstrated that GB-fed pigs displayed less severe pathological changes associated with PRRSV infection compared to the control group, indicating that GB promotes PRRSV clearance. These antiviral effects in pigs may be related to the ability of GB to increase CD3+CD8+ T lymphocyte production observed in the mice. Hence, this mineral may be an effective feed supplement for increasing immune activity and preventing disease. 相似文献
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基因枪与肌肉注射猪繁殖与呼吸综合征病毒ORF5基因疫苗诱导小鼠体液及细胞免疫的比较研究 总被引：8，自引：2，他引：8
猪繁殖与呼吸综合征病毒ORF5基因疫苗（pcDNA—PRRSV—ORF5）以不同免疫途径（基因枪和肌肉注射）免疫BALB／c小鼠，以PBS和空载质粒pcDNA3．1（＋）为对照，采用流式细胞仪（FACS）、淋巴细胞增殖试验（MTT法）及间接ELISA试验分别对小鼠外周血中CD4^＋、CD8^＋T淋巴细胞数、T淋巴细胞的转化功能及小鼠血清中特异性PRRSV血清抗体IgG动态变化进行了检测。结果表明，pcDNA—PRRSV-ORF5基因疫苗接种小鼠后外周血对ConA有明显的反应性，试验组与对照组比较差异极显著（P〈0．01）或显著（P〈0．05），CD4^＋T淋巴细胞数在免疫后7d高于对照组（P〈0．01），CD8^＋T淋巴细胞在免疫后28d高于对照组，不同途径基因疫苗接种小鼠后均诱导小鼠产生PRRSV特异性IgG。在诱导细胞免疫方面，基因枪和肌肉注射各组间无明显差异；在诱导体液免疫方面，基因枪法优于肌肉注射。研究表明制备的pcDNA—PRRSV—ORF5基因疫苗免疫小鼠能够诱导其机体产生良好的体液和细胞免疫应答，基因枪法较肌肉注射更能诱导体液免疫应答的产生。 相似文献
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T M Jacks E Frazier F R Judith G Olson 《American journal of veterinary research》1988,49(11):1832-1835
The influence of efrotomycin administered at the rate of 16 mg/kg of feed in 10 Salmonella typhimurium-inoculated pigs was determined by comparing this group with a group of 10 pigs inoculated with S typhimurium that were given nonmedicated feed. Two control groups of 4 noninoculated pigs each, 1 group medicated with efrotomycin at 16 mg/kg of feed, the other nonmedicated, also were evaluated. An inoculum of 1.7 x 10(10) colony-forming-units/pig induced colonization of S typhimurium in all 20 pigs. Evaluation of the quantity of shedding did not reveal a clear or consistent treatment-related increase in S typhimurium counts; mean differences between the nonmedicated and medicated groups never exceeded 1 log unit. On the last day of the study (day 56 of the medication), 8 nonmedicated and 9 medicated pigs were determined to be infected with S typhimurium via enrichment procedures, so there was no difference in duration of shedding, and there were no significant differences in prevalence of shedding between the nonmedicated and medicated groups at any of the sampling times. Of 1,340 S typhimurium colonies isolated from the nonmedicated and medicated groups, 1,330 were susceptible to all 12 antibacterials tested, indicating no treatment-related effect on susceptibility. At necropsy, S typhimurium was not isolated from any liver or spleen specimens, and was isolated from only 2 of 20 lymph nodes. However, S typhimurium was isolated via enrichment from the cecal contents from all 20 pigs. There were no treatment-related differences in feed consumption, weight gain, or feed efficiency. Appreciable differences in the measurements were not found between the efrotomycin-medicated and nonmedicated pigs. 相似文献
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试验旨在研究羧甲基葡聚糖（CMG）注射剂药效,从而为临床动物疾病防制提供试验依据。试验选取小鼠作为试验动物,进行急性毒性试验及体内抗金黄色葡萄球菌、无乳链球菌及大肠杆菌感染试验。另外,采用MTT方法测定了CMG对奶牛淋巴细胞转化率影响,并对CMG注射剂进行了奶牛临床初步应用试验。结果表明,小白鼠对CMG最大耐受量在240 mg/只以上,且在4 mg/只浓度下对金黄色葡萄球菌、无乳链球菌及大肠杆菌感染的保护率分别为60%、62.5%、87.5%;CMG能明显提高奶牛淋巴细胞转化率,临床应用试验组比空白对照组隐性乳腺炎发病率下降26.8%。本试验结果表明,CMG注射液可作为潜在新型兽用免疫增强剂在动物临床应用。 相似文献
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口蹄疫疫苗非抗原蛋白对146S抗原免疫效果的影响 总被引：1，自引：1，他引：0
为探究非抗原蛋白对口蹄疫病毒A型(FMDV-A) 146S抗原免疫效果的影响,本研究以小鼠和猪为试验动物,分别制备5组小鼠注射用样品:A组(3μg 146S抗原)、B组(3μg 146S抗原+25μg非抗原蛋白)、C组(3μg 146S抗原+50μg非抗原蛋白)、D组(3μg 146S抗原+100μg非抗原蛋白)及E组(空白对照,PBS);同时制备4组猪注射用样品:A组(18μg 146S抗原)、B组(18μg 146S抗原+400μg非抗原蛋白)、C(18μg 146S抗原+4 000μg非抗原蛋白)及D组(空白对照,PBS)。免疫接种试验动物后,通过淋巴细胞增殖试验及实时荧光定量PCR评价比较不同疫苗样品组小鼠产生的细胞免疫应答水平,同时通过液相阻断ELISA方法检测、评价各试验组动物(小鼠和猪)产生的体液免疫应答水平。小鼠试验结果显示,在免疫后3个检测时间点内,4个试验组中C组的平均抗体水平最高,平均抗体效价分别为4.13、5.83和5.50,而D组的抗体水平最低,平均抗体效价分别为3.46、5.16和4.46;C组细胞增殖能力及Th-1型细胞因子IL-6、IFN-β和TNF-αmRNA表达量均高于其他组。猪试验结果显示,A组和B组间平均抗体水平差异不显著(P>0.05),但A组和B组的平均抗体效价均极显著高于C组(P<0.01)。综合上述结果表明,一定范围内少量非抗原蛋白对FMDV-A 146S抗原的免疫效果没有影响,而高浓度的非抗原蛋白则抑制FMDV-A 146S抗原的免疫效果。 相似文献
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A Kumar M K Oaks K J Kelly 《Comparative immunology, microbiology and infectious diseases》1992,15(2):97-101
The effects of cimetidine, an H2 receptor antagonist, on subpopulation of splenic lymphocytes were studied in mice. Cimetidine (50 mg/kg) was given to groups of mice by intraperitoneal injection. The splenic mononuclear cells from treated and control animals were evaluated for relative number of lymphocyte subpopulations (i.e. LYT1, LYT2, L3T4 and GAM cells) by flow cytometry. The percentages of LYT2 cells (suppressor equivalent) were significantly lower (5.5 vs 12.0%) in mice who were treated with cimetidine vs control animals. The percentages of L3T4 (helper equivalent) were not statistically different in any of the experimental groups. 相似文献