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1.
用消化法所得的猪旋毛虫、犬旋毛虫、旋毛形线虫 (Trichinellaspiralis)和本地毛形线虫 (Trichinellanativa)分别感染健康猪。结果表明 :4个旋毛虫隔离种对猪的感染性存在着明显差异 ,猪旋毛虫和T .spiralis对猪易感 ,其繁殖力指数 (RCI)分别为 385 .6 8± 41.5 1和 30 0 .5 5± 12 .45 ;而犬旋毛虫和T .nativa对猪不易感 ,RCI分别是 0 .0 6 4± 0 .0 31和 0 .0 33± 0 .0 33。结果揭示黑龙江省猪旋毛虫相当于T .spiralis,犬旋毛虫相当于T .nativa ;犬旋毛虫和T .nativa很难通过猪的感染而对人体健康构成威胁。  相似文献   

2.
Experimental trichinosis in sheep.   总被引：1，自引：1，他引：0       下载免费PDF全文
Trichinella spiralis spiralis infections were established in sheep by administering infective larvae via gavage or feeding infected musculature. Trichinella spiralis nativa infective larvae had a low infectivity for sheep although light infections may be established in some animals with large infective doses. For the most part, sheep were averse to ingesting musculature mixed in a grain ration unless it was camouflaged with molasses. The heaviest infections usually occurred in the masseter muscle. The fact that sheep are averse to ingesting muscle tissue may reduce the likelihood of trichinosis. Anti-Trichinella antibodies to both T: spiralis spiralis and T. spiralis nativa were produced as demonstrated by the enzyme-linked immunosorbent assay. Seroconversion occurred in several sheep challenged with T. spiralis nativa even though larvae were not recovered from the musculature by pepsin-digestion.  相似文献   

3.
Rats and pigs were vaccinated against Trichinella spiralis spiralis either by feeding infective larvae of the subspecies, Trichinella spiralis nativa in musculature or by gavage. The number of larvae established in the musculature of vaccinated nonchallenged and vaccinated challenged rats and pigs were negligible and statistically comparable, while highly significant infections were established in the nonvaccinated challenged rats and pigs. High vaccination doses of T. spiralis nativa gave virtually complete protection to challenge with T. spiralis spiralis in pigs. The results of one trial in rats with a lower vaccination dose of larvae suggest that there is a minimal vaccination dose of larvae required to elicit marked resistance to challenge. The low numbers of muscle larvae established due to the high vaccination doses of larvae confirm the low infectivity of the subspecies, T. spiralis nativa in rats and pigs.  相似文献   

4.
旋毛虫各隔离种对小鼠的感染性研究   总被引：1，自引：0，他引：1  
比较研究了黑龙江省猪、犬旋毛虫及国际标准虫种旋毛形线虫(Trichinella spiralis)和本地毛形线虫(Trichinella nativa)对小鼠的感染性异同。结果表明，四者对小鼠的感染力存在着显著差异，猪旋毛虫和旋毛形线虫在小鼠体内的繁殖力指数(RCI)分别为121.01±7.80和149.86±7.47；而犬旋毛虫和本地毛形线虫的RCI分别为60.98±5.05和55.15±4.69。由实验结果可认为黑龙江省的猪旋毛虫相当于旋毛形线虫，而犬旋毛虫相当于本地毛形线虫。  相似文献   

5.
The influence of preconditioning temperature, length of the preconditioning period, host and age of the infection on the survival of Trichinella spiralis nativa larvae in musculature to low temperature refrigeration was investigated. Dogs, foxes, ferrets, mink and guinea pigs were infected with a T. spiralis nativa isolate, killed at various times postinfection, preconditioned at temperature of -10 degrees C, -15 degrees C or -20 degrees C for varying periods of time prior to low temperature refrigeration and subsequent pepsin digestion to determine survival of larvae. The preconditioning temperature played an important role in the subsequent survival of larvae in musculature at low refrigeration temperatures. Under the conditions of this study, survival of larvae was greater as the preconditioning temperature became lower. The minimum period of preconditioning required had an inverse relationship with the refrigeration temperature. Preconditioning of the T. spiralis nativa isolate used occurred in the musculature of guinea pigs, foxes, ferrets, mink and dogs with larvae surviving longer in vulpine and canine musculature than in the other hosts studied. Age of the infection was not a major factor in the survival of preconditioned larvae in musculature at low refrigeration temperatures although survival was slightly longer in older infections.  相似文献   

6.
Enzyme immunoassays using the triple antibody enzyme linked immunosorbent assay (ELISA) with both Trichinella spiralis spiralis and T. spiralis nativa excretory-secretory (ES) antigens and a commercial Trichinella spiralis enzyme immunoassay test kit were carried out on sera from pigs that were infected with light, moderate and high doses of infective T. spiralis spiralis and T. spiralis nativa respectively. Seroconversion occurred in all pigs given infective Trichinella larvae although no trichinae were recovered from pigs given T. spiralis nativa larvae and examined between days 92 and 99 postinfection by pepsin digestion. Anti-Trichinella antibodies were detected in pigs infected with T. spiralis spiralis and T. spiralis nativa by ELISA using either the homologous or heterologous ES antigen. The commercial Trichinella spiralis enzyme immunoassay test kit also detected anti-Trichinella antibodies in both the T. spiralis spiralis and T. spiralis nativa infected pigs. The commercial test kit did not appear to be as sensitive as the triple antibody ELISA since it usually took two to three days longer for seroconversion to be detected by the former procedure. Finally seroconversion occurred more rapidly in swine infected with T. spiralis spiralis than with pigs receiving comparable doses of T. spiralis nativa.  相似文献   

7.
Inoculation of swine with a sylvatic isolate of Trichinella spiralis, designated T s nativa, resulted in low numbers of muscle larvae, compared with muscle larvae accumulation in swine inoculated with a pig type of T s spiralis. Despite low infectivity of T s nativa for swine, primary inoculation resulted in high levels of immunity against challenge infection with T s spiralis. This immunity was expressed in accelerated expulsion of challenge adults from the intestine and reduced numbers of muscle larvae. Pigs inoculated with T s nativa developed cellular and humoral responses similar to those in pigs inoculated with T s spiralis. However, in immunoblots, sera from pigs inoculated with T s nativa recognized additional proteins in muscle larvae excretory-secretory (ES) products, compared with sera from pigs inoculated with T s spiralis. Active immunization of pigs with ES products from T s nativa resulted in numerically higher, but not significantly different levels of immunity, compared with pigs immunized with ES from T s spiralis. The highest levels of immunity were obtained in pigs immunized with a T s spiralis newborn larval extract. The combination of ES products and newborn larval extract did not result in additive levels of immunity. These results indicate that the major immune effector response to Trichinella sp in pigs is against the newborn larvae, regardless of the genetic type of Trichinella sp.  相似文献   

8.
旋毛虫病McAb快速ELISA诊断盒的应用研究   总被引：3，自引：0，他引：3  
应用ＭｃＡｂ 快速ＥＬＩＳＡ 诊断盒,对感染了４ 个旋毛虫隔离种的猪定期检测其血清中抗体出现情况。结果,猪旋毛虫和旋毛形线虫（Ｔ．ｓｐｉｒａｌｉｓ）在感染２４ ｄ 后、犬旋毛虫和本地毛形线虫（ Ｔ．ｎａｔｉｖａ）３１ ｄ 后,可在猪血清中检出抗体;在抗体出现时间上前二者较后二者早７ ｄ 左右。  相似文献   

9.
猪、犬旋毛虫DNA限制性片段长度多态性分析   总被引：3，自引：0，他引：3  
以旋毛虫国际标准虫种Ｔ．ｓｐｉｄａｉｓ和Ｔ．ｎａｔｉｖａ作对照,应用ＤＮＡ限制性片段长度多态性（ＲＦＬＰ）技术对黑龙江省猪、大旋毛虫进行虫种鉴定。结果显示：猪旋虫和Ｔ．ｓｐｉｒｌａｉｓ酶切图谱相同;犬旋毛虫和Ｔ．ｎａｔｉｖａ酶谱一致,结果提示,黑龙江猪旋毛虫为Ｔｓｐｉｒａｌｉｓ,犬旋毛虫为Ｔ．ｎａｔｉｖａ。  相似文献   

10.
以旋毛虫国际标准虫种：旋毛形线虫（Trichinella spirlais）和本地长形线虫(Trichinella nativa）作对照,应用DNA限制性片段长度多态性（PELP）技术对黑龙江省猪、犬旋毛虫进行虫种鉴定。结果显示：猪旋毛虫和旋毛形线虫酶切图谱相同;犬旋毛虫和本地毛形线虫酶谱一致。结果提示,黑龙江猪旋毛虫为旋毛形线虫,犬旋毛虫为本地毛形线虫。  相似文献   

11.
Preconditioning of a Trichinella spiralis nativa isolate in ferret and fox musculature was carried out by freezing at -15 degrees C from 0 to 322 days prior to low temperature refrigeration at -32 degrees C. A limited number of preconditioned samples of infected fox musculature was also refrigerated at -45 degrees C. Preconditioned larvae were appreciably more resistant than those subjected to the low temperatures directly. Under the conditions of this investigation, the longer the period of preconditioning, the greater the resistance, (i.e., survival of larvae) observed. The larvae in fox musculature were slightly more resistant than those in ferret musculature. Limited infectivity trials indicated that pre-conditioned larvae surviving low temperature refrigeration of -32 degrees C -45 degrees C retained their infectivity for at least 44 and 37 days, respectively.  相似文献   

12.
A refrigeration technique to differentiate the subspecies, Trichinella spiralis spiralis and T. spiralis nativa is described. Trichinella spiralis spiralis trichinae in musculature do not survive 48 hours post-refrigeration at -32 degrees C while T. spiralis nativa will survive 72 hours and longer at the same temperature.  相似文献   

13.
猪旋毛虫对猪,犬的感染性研究   总被引：4，自引：0，他引：4  
用消化法所得的猪旋毛虫蚴分别接种猪，犬，结果表明：猪旋毛虫对猪，犬的感染性存在着明显差异，在猪的繁殖力指数为１１７．０４，而在犬为３０．６０，说明哈尔滨地区猪旋毛早相当于旋毛形线虫；其对猪感染性较高而对犬感染较差，但能通过犬的感染而对人体健康构成威胁。  相似文献   

14.
Trichinella spiralis spiralis infections were established in cattle by gavage and by feeding infected musculature in the ration. Trichinae were present in greatest numbers in masseter, tongue and diaphragm. Trichinella spiralis nativa had a low infectivity to cattle although a light infection was established in one cow by a heavy challenge. Cattle had an aversion to eating musculature unless it was camouflaged with molasses. Clinical signs of reluctance to eat and masticate were observed between 10 and 30 days postinfection. Eosinophil counts started to increase at seven days and peaked at about 30 days postinfection. By day 60 eosinophil counts returned to near preinfection levels but in animals examined greater than 90 days postinfection, the counts were variable. Focal lesions of eosinophilic myositis were observed up to about 90 days postinfection. Little cellular reaction was observed surrounding trichinae after muscle invasion and cyst development was completed except for cysts undergoing disintegration. Seroconversion occurred in all cattle examined between 7 and 14 days postinfection. Seroconversion was associated with IgG1 and IgG2 immunoglobulins. Peak levels of antibody occurred between 30 and 60 days. Cattle examined at 182 and 369 days postinfection showed a gradual decrease in antibody levels over time.  相似文献   

15.
旋毛虫各隔离种雌虫生殖能力的实验研究   总被引：4，自引：0，他引：4  
本试验对旋毛虫各隔离种雌虫体外产新生幼虫能力进行了研究。结果显示，猪旋毛虫和旋毛形线虫(Trichinella spiralis)雌虫体外培养24h平均产新生幼虫数分别为66.00±7.34和76.20±7.57，而犬旋毛虫和本地毛形线虫(Trichinella nativa)分别是28.80±4.30和22.00±3.22，前者在雌虫体外产幼虫能力上明显高于后者。研究结果表明，黑龙江猪旋毛虫相当于旋毛虫形线虫，犬旋毛虫相当于本地毛形线虫。  相似文献   

16.
哈尔滨地区猪旋毛虫对猪,犬的感染性试验   总被引：1，自引：0，他引：1  
用消化法所得的猪旋毛虫蚴分别接种猪，犬。结果表明，猪旋毛虫对猪，犬的感染性存在着明显差异，在猪的繁殖力指数为１１７．０４，而在犬为３０．６０，说明哈尔滨地区猪旋毛虫档地旋毛形线虫；  相似文献   

17.
A tongue biopsy technique developed for the detection of Trichinella spiralis infection in swine involves taking a deep core biopsy of the tongue musculature, and examination of the sample by digestion. Using this procedure, 31 of 52 (60%) swine from an Indiana herd were found to be infected with T. spiralis. The average biopsy weighed 0.42 g, and the intensity of infection averaged 180 larvae per gram (range 2-1157). The biopsy was quick and easy to perform and the tongues healed well following the procedure. This technique may have applicability for Trichinella detection in epidemiological, control and research studies on swine and other animals.  相似文献   

18.
Enzyme-linked immunosorbent assay examination of sera from pigs vaccinated with T. spiralis nativa infective larvae and/or challenged with T. spiralis spiralis larvae using a T. spiralis spiralis excretory-secretory antigen showed a significant cross-reaction between the two species of Trichinella. Eight of 12 pigs vaccinated with a high dose of T. spiralis nativa reacted positively 28 days postvaccination while the remaining four pigs had high but negative ELISA optical density readings. Five of six pigs challenged with the homologous species reacted positively 28 days postchallenge but the sixth pig remained negative despite having a muscle infection of 5.6 larvae/g of musculature.  相似文献   

19.
本地毛形线虫49 Ku ES蛋白结构基因的分子克隆及原核表达   总被引：1，自引：0，他引：1  
提取Trichinella nativa(T.nativa)肌幼虫的总RNA,用RT-PCR方法扩增出了编码T.nativa 49 Ku ES蛋白的结构基因。基因克隆后测序,序列测定结果表明:目的基因TNPG长度为951 bp,核苷酸序列同已发表的Trichinella spiralis(T.spiralis)相应的序列P49同源性为97.68%,所推导的氨基酸序列同源性为95.24%。将目的基因TNPG插入到原核表达载体pET-30a的BamHⅠ酶切位点处,并转化到感受态表达菌中进行诱导表达。结果显示TNPG在原核表达菌BL-21中获得了高效表达,表达产物为40.8 Ku的融合蛋白,表达量达到菌体总蛋白的22.8%。通过Western blot分析,表达产物可以被小鼠T.nativa和T.spiralis阳性血清以及它们的中国地理株的小鼠血清特异性识别。  相似文献   

20.
The study of Trichinella isolates from wildlife in Germany revealed the presence of Trichinella spiralis and Trichinella britovi in wild boars and foxes. T spiralis was detected in meat products imported from Spain, which is one of the two endemic areas of domestic trichinellosis in the European Union: It was also detected in meat from a grizzly bear marketed in Alaska, and Trichinella nativa was detected in a polar bear from the Berlin Zoo. These results stress the importance of examining for Trichinella live animals and meat products imported to Germany from both EU and non-EU countries. Furthermore, carnivores from Arctic regions that are born in the wild and placed in zoos can represent a risk for the introduction of the freeze-resistant species of Trichinella in a new region if, once the animal dies, the carcass is not properly destroyed.  相似文献   

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