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1.
AIM: To investigate the effect of Jagged1 overexpression on endothelial cell-directional differentiation of aged rat-derived endothelial progenitor cells (EPC).METHODS: Mononuclear cells were obtained from bone marrow of young (1 to 2 months old) or aged (19 to 26 months old) Sprague-Dawley rats and cultured in DMEM/F12 medium supplemented with 10% FBS. EPC were characterized as double positive for DiI-ac-LDL uptake and lectin binding. The experiments were divided into control group, PIRES2-EGFP transfection group, PIRES2-EGFP-Jagged1 transfection group and young rat-derived EPC group in which transfection was not performed. The GFP expression positive cell number was acquired by fluorescence microscopy and the transfection efficiency was calculated. Immunofluorescence, RT-PCR and Western blotting were used to detect the mRNA and protein expression. In vitro vasculogenesis kit was used to test the tube formation ability of EPC.RESULTS: EGFP-Jagged1 transfection induced a significant increase in the expression of Jagged1 in aged rat-derived EPC (P<0.01). Compared with the control, Jagged1 overexpression markedly enhanced the mRNA expression of von Willebrand factor (vWF) and kinase insert domain receptor (KDR) of vascular endothelial grouth factor vWF in aged rat-derived EPC (P<0.01) and improved the EPC-related tube formation (P<0.01). No significant difference between Jagged1 transfection and young rat-derived EPC groups in vWF and KDR mRNA expression and the ability of tube formation was found. CONCLUSION: In endothelial cell-conditioning medium, Jagged1 overexpression significantly promotes aged rat-derived EPC differentiation into mature endothelial cells.  相似文献   

2.
AIM: To evaluate the mobilization of endothelial progenitor cells (EPCs) in mice peripheral blood during hindlimb ischemia alone or in combination with granulocyte colony stimulating factor (G-CSF). METHODS: Hindlimb ischemia was established in mice by surgical excision of both femoral arteries. Fluorescence-activated cell sorting (FACS) was used to detect the expression of cell-surface CD34 and vascular endothelial growth factor recepter-2 (VEGFR2) antigens. The ratio of double-positive cells for CD34 and VEGFR2 was regarded as the level of EPCs in peripheral blood. In G-CSF administration in combination with hindlimb ischemia group, the percentage of double-positive cells was also detected. RESULTS: As compared with control group, hindlimb ischemia increased the percentage of EPCs in mice peripheral blood. The hindlimb ischemia combined with G-CSF administration significantly enhanced the percentage of EPCs. CONCLUSION: Ischemia increases the number of EPC in peripheral blood. It may induce the migration of EPC from barrow to peripheral blood. By mobilizing barrow, G-CSF enhances this effect.  相似文献   

3.
AIM To investigate the role of monocyte chemoattractant protein-1 (MCP-1) and its receptor CC chemokine receptor 2 (CCR2) in ethanol-promoted breast cancer angiogenesis and the underlying mechanism. METH?ODS: A mouse model of transplanted breast tumor with moderate alcohol consumption was established. The correlations between the expression of MCP-1/CCR2 and the expression of angiogenesis markers [platelet endothelial cell adhesion molecule-1 (PECAM-1) and vascular endothelial growth factor (VEGF)] in tumor tissues were examined by immunohistochemistry. In vitro, a 3D tumor-endothelial co-culture system was established to observe tumor angiogenesis and the role of MCP-1/CCR2 signaling pathway in alcohol-mediated angiogenesis. The cell migration ability was detected to clarify whether MCP-1/CCR2 enhanced cell mobility to form new vessels. RESULTS MCP-1 and CCR2 were both highly expressed in the breast tumor tissues of tumor-bearing mice consuming alcohol, and their expression levels were consistent with the angiogenic markers PECAM-1 and VEGF (P<0.05). The interaction between mouse breast cancer E0771 cells and endothelial cells was observed to promote angiogenesis in the 3D tumor-endothelial co-culture system with or without alcohol stimulation. MCP-1 promoted this kind of tumor angiogenesis, while CCR2 antagonist effectively inhibited the tumor angiogenesis and especially blocked alcohol-induced angiogenesis. Activation of MCP-1/CCR2 signaling pathway enhanced the migration ability of endothelial cells. CONCLUSION The MCP-1/CCR2 signaling pathway plays an important role in promoting the angiogenesis of breast cancer stimulated by alcohol. The mechanism might be that MCP-1 improves the migration of endothelial cells and then promotes angiogenesis.  相似文献   

4.
MicroRNAs (miRNAs)are a class of non-coding, endogenous, single-stranded small RNA molecules composed of 19~25 nucleotides. miRNAs are widely involved in the process of human life activities. Recent studies have shown that part of miRNAs regulate the vascular endothelial function and angiogenesis. High expression of miRNA-21 is found to play important roles in the cell proliferation, cell apoptosis, cell growth and death of vascular endothelial cells. This review will focus on the recent progress related to miRNAs in vascular endothelial function and angiogenesis, providing a new insight in cardiovascular disease prevention, clinical diagnosis, prognosis and target therapeutics.  相似文献   

5.
AIM: To establish a three-dimensional angiogenesis model in vitro for observing the influence of tumor cells on angiogenesis, and to explore its possible molecular mechanism. METHODS: Human umbilical vein endothelial cells (HUVECs) and mouse endothelial cells SVEC4-10EE2 were coated on the surface of beads, and then mixed with fibrinogen and seeded in cell culture plates containing thrombin. The cultured endothelial cells coated on the beads grew into a vessel-like structure in a three-dimensional space containing fibrin condensate to establish an in vitro three-dimensional angiogenesis model. Tumor cells MDA-MB-231 and E0771 were co-cultured in the model to observe the effect of tumor cells on angiogenesis in vitro,respectively. The concentrations of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) in the tumor cells culture supernatant were measured by ELISA. An antagonist of CCR2 (receptor of MCP-1), along with SU5416 (an inhibitor of VEGF receptor), were added to the tumor cell co-culture system. The supernatant of the tumor cells was collected as a conditioned medium, which was then added to the angiogenesis system of HUVECs or SVEC4-10EE2 cultured individually. The effect of conditioned medium on angiogenesis was observed under the conditions of with or without SU5416, as well as with or without CCR2 antagonist. RESULTS: Under normal condition, HUVECs and SVEC4-10EE2 formed a multi-cellular vascular structure the three-dimensional angiogenesis model in vitro. The co-culture of MDA-MB-231 (E0771) cells significantly promoted in the formation of membrane-like structures. The ELISA results showed that the levels of MCP-1 and VEGF in the supernatant of tumor cells were significantly elevated (P<0.05). MCP-1 promoted the formation of membrane tube in three-dimensional culture system in vitro. After adding the antagonists of MCP-1 receptor CCR2 and VEGF (SU5416), the angiogenesis was significantly inhibited (P<0.05). At the same time, conditioned medium promoted the formation of extracorporeal membranes in the endothelial cells. The promoting effect was blocked by CCR2 antagonists and SU5416 (P<0.05). CONCLUSION: The in vitro three-dimensional angiogenesis model is successfully established. Tumor cells significantly promote the formation of membrane tubes. The effect of tumor cells might be related to MCP-1 and VEGF secretion.  相似文献   

6.
AIM: To investigate whether homocysteine (Hcy) has influences on endothelial progenitor cell (EPCs) number and activity from peripheral blood. METHODS: Total mononuclear cells (MNCs) were plated on fibronectin-coated culture dishes and cultured for 7 days, and then attached cells were stimulated with Hcy or vehicle control for 6 h, 12 h, 24 h and 48 h. The adhesion, proliferation, migration and in vitro vasculogenesis activity of EPCs were assayed, respectively. RESULTS: Incubation of isolated human MNCs with Hcy dose and time-dependently decreased the number of EPCs with maximum at 200 μmol/L for 24 hours (35.7±6.7 vs 62.5±10.6， P＜0.01). In addition, Hcy impaired EPC proliferative (0.531±0.061 vs 0.328±0.055， P＜0.05), migratory (26.3±6.4 vs 6.4±3.7， P＜0.01), adhesive (33.1±8.1 vs 17.4±7.5， P＜0.01) and vasculogenesis capacity (25.4±9.1 vs 10.4±4.7， P＜0.01) in a dose and time-dependent manner. CONCLUSION: It is suggested that Hcy may result in the reduction of EPCs and decrease EPC functional activity.  相似文献   

7.
Limitations against propagation of Cola nitida include pronounced dormancy of between 1 and 8 months after sowing (MAS) leading to uneven germination and seedling growth. This experiment was conducted in a screenhouse, Federal University of Agriculture Abeokuta, Nigeria, to evaluate the effects of mini-nut and nut-color on germination and seedling growth of C. nitida nuts. The experiment was in a completely randomized design replicated 3 times. Treatments comprised two factors: nut/seed-colored biotype (white, pink, and red) and mini-nut (embryonic portion of cotyledon) [excised embryonic portion cotyledon (EEPC), 25% embryonic portion cotyledon (EPC), 50% EPC, 75% EPC, and 100% EPC (whole nut/seed)], giving 15 treatment combinations sown in plastic cups of 50 cl filled with sawdust. Germination percentage and morphological growth of plantlets (plant height (cm), number of leaves, dry matter yield (g/plant)) were monitored; data subjected to analysis of variance and mean comparisons done (p ≤ 0.05). As early as 4 WAS, the EEPC and 25% EPC had 67.78% and 54.44% germination, respectively. Within the same period, 51.33%, 48.00%, and 31.33%, respectively, of white-, pink-, and red-colored seeds germinated. At 6 WAS, the white-, pink-, and red-colored seeds had mean germination of 80.67%, 92.00%, and 72.67%, respectively. At 12 WAS, germination was observed to complete with the pink-, red-, and white-colored nuts having 97.33%, 92.61%, and 91.33%, respectively. Within the same period, the mini-nut sizes had between 92.22% and 95.56% germination. Combining the two factors, 50% EPC, 25% EPC, and EEPC of white-nut biotype germinated earliest with 50.00–76.67% at 4 WAS and 73.33–80.00% at 5 WAS. The EEPC of pink-colored biotype attained 100% at 6 WAS, while other treatments were between 56.67% and 93.33%. Thus, while earliness in germination pattern follows the order: EEPC > 25% EPC > 50% EPC > 75% EPC > whole nut, the plantlet morphological growth followed a reverse order. With mini-nut technique therefore, timely and even germination of kola nuts were obtained and 50%EPC would be recommended for conventional propagation while EEPC would require nutrient amendment for improved growth.  相似文献   

8.
AIM：To investigate the cytological basis and differentiating conditions of human bone marrow mesenchymal stem cells(hMSCs) differentiated into cells of the endothelial lineage in vitro.METHODS：hMSCs were isolated by density gradient centrifugation and fractionated on a 1 073 g/L Percoll.The combination of VEGF165 and various matrix proteins including fibronectin (FN) and typeⅠ collagen (Col) was used to induce hMSCs in vitro.Cells were characterized by immunohistochemistry,cytochemistry,FACS and ultrastructure to identify and detect the differentiated population and markers.RESULTS：hMSCs was positive for KDR.PAS reaction was positive and ultrastructure of hMSCs showed glycogen-pool in ectoplasm.Glycogen reducing or disappear suggested that stem cells have occurred differentiation.Induction of hMSCs resulted in the increase of KDR,β1 integrin and CD34.CONCLUSION：hMSCs were induced to a transit population (TP) that differentiated toward the endothelial progenitor cells (EPC),but not a really EPC.hMSCs pedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells (ECs).  相似文献   

9.
Tryptase, a predominant serine protease in mast cells, is released by mast cell degranulation in an enzymatically active form. Recent research suggests that tryptase plays important roles in mast cell-mediated anaphylaxis, angiogenesis, initiation and promotion of neoplasm. This paper reviews tryptase.s struture, molecular biology, biochemistry, processing, activation and its relationship with diseases.  相似文献   

10.
MO Qian  LI Dan  LING Wen-hua 《园艺学报》2012,28(7):1187-1191
AIM: To study the effect of miR-18a on angiogenesis of human aortic artery endothelial cells. METHODS: After 10 nmol/L miR-18a mimics or 20 nmol/L inhibitor was transfected into human aortic endothelial cells, the expression level of miR-18a was determined by qRT-PCR, and the capacities of endothelial cell angiogenesis, such as migration, adhesion and tube formation, were observed. RESULTS: Forty-eight hours after transfection with miR-18a mimics, the expression of miR-18a was as 608 folds as the control (P<0.05), but decreased to 31% of the control level in miR-18a inhibitor transfection group (P<0.05). Compared with control group, the numbers of endothelial cells, which migrated to the lower Transwell chamber and formed capillary-like tubes, declined by 60% and 52%, respectively, in miR-18a mimics group (P<0.01), and they increased by 100% and 84%, respectively, in miR-18a inhibitor transfection group (P<0.05, P<0.01). In addition, the inhibitor treatment group displayed more potent adhesion capacity, 43% higher than that in control group (P<0.05). CONCLUSION: miR-18a is involved in angiogenesis of human arterial endothelial cells, and might be a potential molecular therapeutic target of vascular diseases.  相似文献   

11.
The protein of BRCC36 is a kind of enzyme specifically hydrolyzing K63-linked poly-ubiquitin chain and widely found in a variety of eukaryotic cells. BRCC36 recognizes diverse substrate proteins, and participates in various kinds of pathophysiological responses such as DNA damage repair, cell signal transduction and cell cycle control. It plays an important role in the process of cancer, angiogenesis and cardiac injury. This review discusses the progress in the investigation on BRCC36 protein to provide the necessary information for searching new therapeutic targets of many diseases.  相似文献   

12.
李平  包隆桦 《现代园艺》2011,(11):147-148
园林景观建设是发展城市品味和文化建设的需要。要搞好园林景观建设,就必须抓好园林景观工程的施工管理。分析了园林景观工程的特点,并对园林工程中实行EPC总承包管理模式提出了几点看法。  相似文献   

13.
仙客来实生黄化叶柄培养的研究   总被引：13，自引：0，他引：13  
对仙客来实生黄化叶柄形成器官的能力及消除内生菌的污染效果进行了研究。结果表明,以实生黄化叶柄为外植体进行培养,不仅完全可以消除内生菌的污染,而且诱芽率( 75.6％)是普通叶柄培养的5.3倍,产生的总芽数是普通叶柄的11.5倍,诱根率(59.3％)也是普通叶柄培养的1.9倍。这种培养方法,必将成为今后无性繁殖仙客来的一条有效途径。  相似文献   

14.
Angiogenesis is the process of capillary formation from the existing blood vessels, which is regulated by many cytokines. Balance of these cytokines plays an important role in angiogenesis. Unbalance of these cytokines, leading to excessive or insufficient blood vessel, relates to a variety of diseases, such as tumor, ophthalmic diseases and wound healing. Recently, it has been observed that angiogenesis is also involved in Parkinson's disease and Alzheimer's disease. This review mainly discusses the molecular mechanism of angiogenesis and related diseases, and emphasizes the value of targeting angiogenesis as a strategy to develop drugs for those diseases.  相似文献   

15.
GUO Hui  YANG Hui-ling  ZHENG Qin 《园艺学报》2005,21(11):2178-2182
AIM: To investigate the angiogenesis in the process of sarcoma 180 (S180) tumor transplantation and changes of regulator factors, and explore the possible mechanism. METHODS: The S180 transplanted tumor in the Km mouse was used to detect the tumor angiogenesis by immunohistochemical examination of FⅧ. The levels of VEGF (V) and endostatin (E) in serum and the homogenate of tumor tissue were measured by ELISA and EIA, and the correlation between tumor weight and microvessel count (MVC) and morphology in tumor was also analyzed by multiple ANNOVA method. RESULTS: MVC, the relative count of total vessels and relative total vessel area increased with the development of transplanted S180. VEGF level in tumor tissue were higher at the 10th and 15th day than the 5th day after tumor transplantation. Endostatin in the tumor tissue and serum both reached the highest level at the 15th day, V/E ratio did not changed in this process. Furthermore, MVC, average vessel area and relative total area had a significant correlation with tumor weight. CONCLUSION: MVC increases in the development of S180 transplantation tumor and is related with the tumor weight; the positive regulator of angiogenesis in the tumor tissue is up-regulated during tumor growth, and the regulators in the tumor tissue maintains a relative balance.  相似文献   

16.
As a human basement membrane-derived inhibitor of angiogenesis and tumor growth, canstatin has been paid great attention since it was isolated and identified in 2000. Canstatin significantly inhibited human endothelial cell migration and proliferation and induced apoptosis, suggesting that it might be a powerful and potential therapeutic molecule for atherosclerosis, unstable angina and tumor.  相似文献   

17.
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18.
DU Bin  ZHOU Xu-long 《园艺学报》2003,19(2):275-278
Neovascularization plays an important role in embyonic development and many diseases. VEGFs and angiopoietins are two known growth factor families that are specific to vascular endothelium. The action of angiopoietins is associated not only with angiogenesis but also with postnatal vasulogenesis. Thus there is a good prospective use for angiopoietins or their antagonists to promote or inhibit angiogenesis in clinical therapy.  相似文献   

19.
AIM:To investigate the effects of atorvastatin reloading in pre-percutaneous coronary intervention (PCI) period on endothelial progenitor cell (EPC) count and inflammatory cytokine expression in the stable angina pectoris patients who had previously received long-term statin treatment. METHODS:The patients with stable angina pectoris that had received long-term statin therapy and planned to accept PCI were randomized into 3 groups: 80 mg atorvastatin 12 h and 40 mg 2 h before coronary angioplasty (80 mg reloading), pre-operatively with 40 mg/d atorvastatin for 7 d (40 mg reloading), and without atorvastatin reloading (no reloading). CD45-/CD133+/CD34+, CD45-/CD34+/KDR+ and CD45-/CD144+/KDR+ EPCs in 100 μL peripheral blood were determined by flow cytometry 1 h prior to PCI and 1 h, 6 h and 24 h after PCI. The serum concentrations of soluble intercellular adhesion molecule 1 (sICAM-1), C-reactive protein (CRP) and troponin I (TnI) were analyzed immediately prior to and 24 h after PCI. RESULTS: (1) In 80 mg reloading group, the numbers of circulating CD45-/CD133+/CD34+ and CD45-/CD34+/ KDR+ early differentiation stage EPCs 1 h and 6 h after coronary angioplasty was significantly elevated compared with those before PCI (P<0.05). (2) In control group, the serum concentrations of sICAM-1 and CRP 24 h after PCI were significantly elevated (P<0.05) compared with preoperative values. (3) The rise in serum TnI concentration from pre- to post-operation in 80 mg reloading group was lower than that in control group. CONCLUSION: The method of atorvastatin reload before PCI affects the number of EPCs in peri-operative period. High dose of atorvastatin application before PCI triggers early EPC circulation. The serum levels of post-operative inflammatory cytokine sICAM-1 as well as CRP are reduced by atorvastatin reloading before PCI.  相似文献   

20.
LIU Jing  FAN Zhong-ze 《园艺学报》2010,26(8):1646-1649
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