共查询到20条相似文献，搜索用时 203 毫秒
1.
AIM: To observe the effect of TNF-α and Yigu capsule drug-containing sera on osteoblasts apoptosis in osteoblasts-osteoclasts coculture system.METHODS: (1) Twenty female Sprague-Dawley rats, 10 months old, were randomly assigned into 2 groups, NS and Yigu capsule groups, to prepare blank sera and drug-containing sera. (2) The DNA gel electrophoresis method was used to detect apoptosis of osteoblasts treated with different concentration of TNF-α in order to determine the best dosage in the co-culture system. (3) The cells were divided into four groups, TNF-α group, normal group, TNF-α + blank serum group and TNF-α + drug-containing serum group. DNA gel electrophoresis, acridine orange staining and flow cytometry were used to observe osteoblast apoptosis in these groups. RESULTS: (1) After induced by TNF-α for 72 h at a concentration of 60 μg/L, relatively typical DNA ladder appeared in TNF-α group. (2) Only two DNA brands appeared and most of cells were well-proportioned stained in TNF-α + drug-containing serum group, the rate of osteoblasts apoptosis in TNF-α + drug-containing serum group (9.60%±0.26%) was obviously lower than that in the TNF-α group (26.90%±0.06%) and TNF-α + blank serum group (18.10%±0.06%). CONCLUSION: TNF-α induces osteoblast apoptosis in the co-culture system, and Yigu capsule drug- containing serum prevents osteoblast apoptosis induced by TNF-α. 相似文献
2.
AIM: The effects of YIGU capsule on proliferation and IGF-I mRNA protein expressions in osteoblasts were studied. METHODS: (1) Forty 12-month old Sprague-Dawley female rats were divided randomly into four groups (YIGU capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-Dawley rat osteoblasts were cultured with different YIGU capsule drug-containing serum at different concentrations and different exposure time. MTT method was used to observe proliferation of osteoblasts. (3) RT-PCR method was used to measure the relative IGF-I mRNA levels and ELISA method was used to measure IGF-I secretion at different exposure time. (4) ELISA method was used to measure IGF-I secretion at different exposure time. RESULTS: (1) Proliferation of osteoblasts was more than the control groups after 48, 72 and 96 h, respectively (P<0.01); (2) The relative IGF-I mRNA levels and IGF-I protein expression were higher than those in control group after 48, 72 and 96 h, respectively (P<0.01 or P<0.05). CONCLUSIONS: It was suggested that YIGU capsule drug-containing serum promoted proliferation, IGF-I mRNA and protein expression. These results may be parts of the mechanisms of YIGU capsule to prevent and treat osteoporosis. 相似文献
3.
AIM:To investigate the effect of yigu caps ule drug-containing serum on proliferation,ALP activity and expression of inter leukin-11 mRNA in Sprague-Dawley rat osteobalsts in the co-culture system.METHODS:(1) Osteoblasts and osteoclasts were isolated from 1 an d 5-day-old Sprague-Dawley rats,respectively.The osteoblasts-osteoclasts co- culture system was built to prevent the two kinds of cells from contact and allo w the media to exchange.The experiment included two groups,drug-containing se ra group and control group.(2) The osteoblasts proliferation,ALP activity and expression of interleukin-11 mRNA were detected by the MTT,4-aminoantipyrine sp ectrometric methods and FQ-PCR，respectively.RESULTS:In drug-containing sera group,the osteoblasts prolifer ation,ALP activity,expression of IL-11 mRNA were higher than those of control group (P<0.05).CONCLUSION:Rat sera containing yigu capsule can obviously enhan ce the osteoblast proliferation,ALP activity and expression of IL-11 mRNA. 相似文献
4.
AIM: The effects of benefiting-bone Capsule (BBC) containing serum on IL-6 mRNA and protein expression in osteoblasts were studied.METHODS: (1) The neonate Sprague-dawley rat osteoblasts were cultured and divided into three groups: group Ⅰ (containing deactivating serum with BBC), group Ⅱ (containing deactivating serum without BBC) and group Ⅲ (DMEM medium group)； (2) RT-PCR was used to measure the relative IL-6 mRNA levels； (3) The radioimmunoassay method was used to examine IL-6 protein in the supernatant of the cultured osteoblasts. RESULTS: (1) The relative IL-6 mRNA levels was lower in group I than the control (P<0.05); (2) The IL-6 protein expression in osteoblasts was also lower in group I than the control (P<0.05).CONCLUSIONS: These results suggest BBC drug-containing serum can down-regulate the expression of IL-6 mRNA and protein in osteoblasts, which may be one of the mechanisms of BBC preventing and treating osteoporosis. 相似文献
5.
AIM: To analyze the regulatory effect of Yigu capsule on core binding factor alpha 1 (cbf α-1) gene expression in bone of ovariectomized osteoporosis (OP) rats. METHODS: Thirty-six 10-month old Sprague-Dawley female rats were randomized into three groups: sham-operated group, model group and drug group. After intervention by the corresponding methods, the femurs were collected, SYBR green Ⅰ fluorescence quantitative PCR technique was applied with the internal control of GAPDH according to the relative quantitative formula (2-ΔΔCt), the differentially expressed multiples between the model group or drug group and sham-operated group were calculated. RESULTS: Quantitative formula analysis showed that the level of cbf α-1 gene expression in bone of model groups was decreased than that in sham-operated rats ［compared with sham-operated group, P<0.01, it was (9.9×105)-(1.6×104) times］. While in drug groups the level of cbf α-1 gene expression was 0.19 to 0.92 times than that in the sham-operated group, no significant difference was observed (P>0.05). CONCLUSION: The results of this study show that cbf α-1 gene expression in bone tissue of ovariectomized OP rat is decreased, and Yigu capsule increases the level of cbf α-1 gene expression in bone of OP, indicating that Yigu capsule induces bone marrow mesenchymal stem cells into osteoblasts. 相似文献
6.
AIM: To investigate the protection of pentoxifylline against spinal cord ischemia/reperfusion injury.METHODS: Rabbits sustained spinal cord ischemia with 45 min cross-clamping of the infrarenal aorta. Groups were as follows: sham operation (n=8); ischemic control (n=20), receiving only vehicle; PTX A (n=20), receiving PTX before clamping and PTX B (n=20), receiving PTX at the onset of reperfusion. Rabbits were evaluated for hind-limb motor function with the Tarlov scoring system at 48 h. Serum was assayed with ELISA for TNF-α and spinal cords were harvest for MPO activity, histopathologic analysis and TUNEL staining. Immunohistochemistry was used for PECAM-1 and caspase-3 detection, and the numbers of necrosic and apoptotic neuron were counted at 12 h, 24 h, 48 h and 72 h of reperfusion. The necrotic and apoptotic neurons were also observed with transmission electron microscope.RESULTS: Improved Tarlov scores were observed in PTX-treated rabbits as compared with ischemic control rabbits at 48 h. The significant reductions of TNF-α in serum, activity of MPO, immunoreactivity of the PECAM-1 and caspase-3 were found in PTX-treated rabbits. The numbers of necrosic and apoptotic neuron were higher in PTX-treated rabbits than that in the ischemic control rabbits (P<0.05). No necrosic and apoptotic neuron were found in the sham operation group. CONCLUSION: PTX induces protection against ischemia/reperfusion injury in the spinal cord, thereby preventing both necrosis and apoptosis. 相似文献
7.
LUO Qiang GAO Jing-ping LIANG Ting ZHANG Ya-qi LI Pei-yao WANG Shao-hua LI Jun ZHANG Rui-hua XU Tong 《园艺学报》2019,35(12):2241-2246
AIM: To investigate the expression and role of transient receptor potential cation channel M2 (TRPM2) in H9N2 swine influenza virus (H9N2-SIV) infection-induced mouse pulmonary microvascular endothelial cell (PMEVC) damage. METHODS: At 24 h and 48 h after PMEVC (3 parallel test in each group) was infected by H9N2-SIV, the superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, reactive oxygen species (ROS) level and nitric oxide synthase (NOS) activity were measured according to the manufacturer's instructions. The ultrastructural changes of PMVEC were observed under electron microscope. Transendothelial electrical resistance (TEER) and the percentage of horse radish peroxidase (HRP) influx was measure by Transwell culture. The expression of TRPM2 at mRNA and protein levels was determined by real-time PCR and Western blot. The apoptosis of PMVEC was observed with Annexin V/PI staining. RESULTS: The SOD and GSH levels were decreased significantly in H9N2-SIV-infected PMEVC as compared with control group(P<0.05). However, NOS activity and ROS level were increased dramatically (P<0.01). The organelles of PMVEC infected by H9N2-SIV were decreased sharply, and vacuolization in mitochondria and apoptosis were also observed under electron microscope. The value of TEER was decreased significantly and the percentage of HRP influx was increased dramatically with the H9N2-SIV infection (P<0.01). The expression of TRPM2 at mRNA and protein levels in H9N2-SIV-infected PMVEC was increased dramatically at 24 h and 48 h as compared with control group (P<0.01). Annexin V/PI double staining showed that the cytomembrane was stained by great green fluorescence and some nuclei were stained by red fluorescence in H9N2-SIV-infected PMVEC. However, less fluorescence stained PMVEC were found in control group. CONCLUSION: The expression of TRPM2 at mRNA and protein levels is increased significantly, which is involved in cell damage and apoptosis induced by oxidative stress of H9N2-SIV infection. 相似文献
8.
AIM:To investigate clinical effect of tongxinluo capsule in treating stable angina pectoris patients,and its influence on endothelial function,superoxide dismutase (SOD) and malondialdehyde (MDA).METHODS:One hundred and twenty-four stable angina pectoris patients were divided into three groups,isosorbide treatment group (41 cases),tongxinluo capsule treatment group (40 cases),tongxinluo and isosorbide treatment group (combined treatment group,43 cases).The serum concentrations of nitric oxide (NO),endothelin-1 (ET-1),SOD and MDA were determined before and after treatment.The data in traetment groups were compared with that in normal control.RESULTS:The symptoms of 3 groups were significantly improved,and the total effective rate of tongxinluo capsule group and combined treatment groups were better than that in isororbide treatment group (85.00% and 88.37% vs 73.17%,P<0.05).Before treatment,the levels of serum NO and activity of SOD in angina patients were lower than that in control group.The serum MDA and ET-1 levels were higher than those in control.The levels of serum NO and SOD activity were increased remarkably after tongxinluo capsule or tongxinluo combined treatment.However,besides the concentration of NO increased after isosorbide treatment,the levels of serum ET-1 and MDA and SOD activity were not changed.CONCLUSIONS:The results suggest that tongxinluo capsule could effectively improve the symptoms of stable angina pectoris,and it is important for tongxinluo capsule to increase NO level and decrease ET-1 product,scavenge free radical and prevent lipid peroxidation. 相似文献
9.
AIM:The aim of the present study is to investigate changes of five biochemical markers of bone turnover during the formation of ovariectomized rat model. METHODS:Three-month-old female SD rats were divided randomly into ovariectomized (OVX) group, sham-operated (sham) group and control group. Five biochemical markers of bone turnover levels, including serum osteocalcin (OC), total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRAP) and hydroxyproline (HYP) were measured before and at 1, 1.5, 2, 2.5, 3 and 4 months after surgery. The right proximal tibias of rats were excised at the same time point for histopathological observation. RESULTS:Serum OC, ALP, BALP, TRAP and HYP levels in OVX group were significantly higher than that in sham group. As regard to the time of peak level arrived, the sequence of changes was as follows: TRAP/HYP→OC→ALP/BALP. There was a significant positive correlation between five markers. The pathological changes of trabecular bone in OVX group were only observed 3 months after surgery. CONCLUSION:The results suggest that postmenopausal osteoporosis has a high bone turnover rate. During the formation of ovariectomized rat model, changes of bone resorption markers precede changes of bone formation markers. Serum OC, ALP, BALP, TRAP and HYP are sensitive to evaluate the bone loss in the earlier stage of postmenopausal osteoporosis. 相似文献
10.
XUE Ying-sheng WANG Jing-feng NIE Ru-qiong WU Wei-kang LUO Han-chuan CHEN Xiao-wei 《园艺学报》2004,20(4):603-607
AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs. 相似文献
11.
YANG Qin LUO Xin-hua GENG Xiao-xia XIE Ru-jia HAN Bing LI Cheng-xiu CHENG Ming-liang 《园艺学报》2005,21(5):975-979
AIM: To investigate the effect of a Chinese medicime, Dan-Shao-Hua-Xian capsule, on liver fibrosis induced by CCl4 by observation of apoptosis and cell cycle variation in the liver cells. METHODS: Animal models were produced through eight-week treatment of the rats with CCl4, alcohol and diet of high fat/low proteins, and then administration of Dan-Shao-Hua-Xian to the rats (1 g/kg) via stomach-tube－pouring for eight weeks was performed. Liver index, serum hyaluronic acid (HA) and glutamate pyruvate transaminase (ALT) were measured and hydroxyproline (Hyp) content in urine were determined. The extent of the liver fibrosis was observed under light microscope and apoptosis and cell cycle were also examined by cytometry between the two groups. RESULTS: Compared to the liver fibrosis group, the liver index, serum HA, ALT in the treatment group decreased, the development of liver fibrosis delayed, the urine Hyp and the number of apoptosed cells and the ratio of G0/G1 cells increased, as well as the S phase cells decreased, yet unable to return to normal. All those changes detected were statistically significant. CONCLUSIONS: The Dan-Shao-Hua-Xian is effective in treating the CCl4-induced liver fibrosis in rats partly by virtue of inhibition of the growth of hepatic stellate cells and induction of apoptosis. 相似文献
12.
AIM: To investigate the effects of rosiglitazone on fibroblast-like synoviocyte (FLS)-induced osteoclastogenesis in rheumatoid arthritis (RA) and the related mechanism. METHODS: RA-FLS were cocultured with peripheral blood monocytes from healthy volunteers in the presence of macrophage colony-stimulating factor (M-CSF) and rosiglitazone. Osteoclasts were assayed by tartrate-resistant acid phosphatase (TRAP) staining. Resorption lacunae area was identified by toluidine blue staining and quantified by image analysis software. The mRNA expression of RANKL and OPG was evaluated by real-time PCR, and the protein levels of RANKL, OPG, p-ERK, p-p38 and p-JNK were measured by Western blot. RESULTS: Compared with control group (without rosiglitazone treatment), rosiglitazone at concentration of 15 μmol/L significantly decreased the number of osteoclasts (P<0.01) and resorption lacunae area (P<0.05). The expression of RANKL at mRNA and protein levels was significantly down-regulated by rosiglitazone at concentration of 15 μmol/L, while the mRNA and protein expression of OPG was up-regulated (P<0.01). Rosiglitazone (15 μmol/L) significantly decreased the protein level of p-ERK (P<0.05), but not the protein level of p-p38 or p-JNK (P>0.05). CONCLUSION: Rosiglitazone inhibits RA-FLS-induced osteoclast formation and its resorption activity by down-regulating RANKL expression and ERK phosphorylation, suggesting that rosiglitazone may inhibit RA osteoclastogenesis and bone resorption. 相似文献
13.
AIM: To investigate the effect of Dan-shao-hua-xian (DSHX) capsule on the expression of the family of microRNA-200 (miR-200s) in rat fibrotic livers. METHODS: Forty male Wistar rats weighing 180 g to 220 g were divided into 5 groups (control group, two model groups and two interference groups). The rats in model groups and interference groups were induced by hypodermic injection of CCl4 for 4 weeks and 8 weeks. The rats in interference groups were also treated with DSHX capsule (0.5 g/kg) once daily for 4 weeks and 8 weeks at the same time. The liver index and serum activity of ALT and AST were analyzed. The liver fibrosis was observed under microscope. Additionally, the expression of miR-200a, -200b, -200c, -141 and -429 was determined by quantitative real-time PCR. RESULTS: The liver index, and serum activity of ALT and AST in model groups and 4-week interference group were obviously higher than those in normal control group. The apparent liver fibrosis was observed in 8-week model group. The expression of miR-200a,-200b, -200c, -141 and -429 in the liver of 8-week model groups was obviously higher than that in control group. CONCLUSION: In the process of liver fibrosis induced by CCl4, the obvious changes of miR-200s may play an important role in the development of liver fibrosis. The miR-200s might be the potential target that DSHX capsule inhibits the process of liver fibrosis. 相似文献
14.
AIM: To study the expression profiles and the role of Ca2+/calmodulin-dependent kinase Ⅱ delta (CaMKⅡδ) during osteoclast differentiation.METHODS: Mouse RAW264.7 cells were induced by receptor activator of nuclear factor κB ligand (RANKL) at 50 μg/L for osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption lacunae examination were performed to verify osteoclast formation. The expression of CaMKⅡδ at mRNA and protein levels was also determined by immunofluorescent cytochemistry, RT-qPCR and Western blot at days 0, 1, 3 and 5.RESULTS: TRAP positive multinuclear cells with bone resorption function were formed after 5 d of induction. The mRNA levels of CaMKⅡδ detected by RT-qPCR were 1.028±0.041, 2.478±0.087, 10.524±1.284 and 42.914±2.667 at days 0, 1, 3 and 5, respectively, while the protein levels of CaMKⅡδ detected by Western blot were 0.762, 0.963, 1.802 and 3.136, respectively. The changes of protein level were also verified by immunofluorescence cytochemistry, in which the fluorescence intensity increased in a time-dependent manner (P<0.05).CONCLUSION: The expression of CaMKⅡδ increases with the differentiation of osteoclasts. CaMKⅡδ may play a key role in the osteoclastogenesis. 相似文献
15.
AIM: To conduct the relevance analysis of serum Dickkopf-1 (Dkk-1) and bone mineral density (BMD) in the different ages of female populations. METHODS: The women volunteers (n=100, 20~80 years old) were selected and divided into young group (20~39 years old), middle age group (40~59 years old) and elderly group (60~80 years old). The serum levels of Dkk-1 in the 3 groups of volunteers were detected by ELISA. In the middle age group, 25 people of 45~55 years old were selected and divided into postmenopausal group and premenopausal group to detect the serum level of Dkk-1 in the 2 groups of volunteers by ELISA. The BMD was measured by the method of dual energy X-ray absorptiometry. The differences of Dkk-1 expression levels among different ages of female populations, and the relevance with BMD were compared. RESULTS: With the increase in age, the serum Dkk-1 expression level increased (P<0.05), and BMD were reduced (P<0.05). The blood level of Dkk-1 and BMD negatively correlated (P<0.05) in the 3 groups of volunteers. The serum levels of Dkk-1 and BMD had stronger negative correlation in postmenopausal women group than that in premenopausal women group. CONCLUSION: With the increase in age, the expression level of serum Dkk-1 increases and the BMD level decreases, which contribute to a risk of osteoporosis. In the same age range, the postmenopausal women express higher level of Dkk-1, and the decreased BMD is more obvious, which contribute to a greater risk of osteoporosis. The increased level of Dkk-1 also inhibits bone formation and promotes bone resorption. It may become a new target for preventing and treating osteoporosis. 相似文献
16.
TU Sheng-hao HU Yong-hong WENG Geng-min LU Fu-er ZHANG Ming-min LAI Xian-yang LIU Pei-lin 《园艺学报》2006,22(4):630-633
AIM: To determine whether triptolide induce apoptosis of synovial cells in collagen-induced arthritis (CIA) in rats. METHODS: The male Wistar rats were used to make CIA models by immunized with Bovine collagen Ⅱ (BCⅡ) in Freund's complete adjuvant (FCA). A total of 20 CIA rats were randomly divided into 2 groups, triptolide group (10 rats) and CIA control group (10 rats). Triptolide group were administered with triptolide at 40 μg/kg body weight intramuscularly every three days. CIA control group and another 10 age-matched normal rats were given normal saline instead. The rats were sacrificed on the 31st day after the triptolide administration. The pieces of synovium of the rat knee joints were harvested. The synovium was examined by HE staining and electron microscope. The apoptosis was tested by TUNEL and flow cytometer. RESULTS: The earlier phase of apoptotic synoviocytes were observed under the electron microscope. The flow cytometry showed that the percentage of the apoptotic cells was (3.98±1.16)% in the triptolide group, (1.83±0.82)% in the CIA control group, and (0.87±0.24)% in the normal group (P<0.01: triptolide vs control group). While the percentage of the cells in DNA synthesis phase was (3.3±1.2)% in the triptolide group, (8.0±1.4)% in the CIA control group, and (3.4±0.7)% in the normal group. There is significantly different in the apoptosis changes between the triptolide group and the CIA control group (P<0.01: triptolide vs CIA control group). The TUNEL labeling demonstrated that the percentage of the apoptotic cells was (4.5±1.0)% in the triptolide group, (2.2±1.0)% in the CIA control group, and (1.0±0.4)% in the normal group. The difference of apoptotic rate between the triptolide group and the CIA control group is significant (P<0.01). CONCLUSION: This study demonstrates that triptolide can induce apoptosis in CIA rats, which may be one of the mechanisms that triptolide treats the rheumatoid arthritis. 相似文献
17.
Prevention and treatment of Hua Yu Dao Zhi decoction in renal fibrosis induced by gentamicin in rats
AIM: To investigate the effects of Hua Yu Dao Zhi decoction (HYDZD) on renal interstitial fibrosis induced by gentamicin in rats. METHODS: 32 healthy male Wistar rats were randomly divided into 3 groups: control group (n=6), gentamicin group (n=13) and HYDZD+gentamicin group (n=13). The renal tubulointerstitial fibrosis in rats was induced by gentamicin for 9 d. All rats were sacrificed on 30 d after modeling and renal tissues were stained with HE. The renal indexes were calculated and the changes of serum creatinine, blood urea nitrogen, total urinary protein in 24 h, and the level of α-SMA, Smad2, Smad7 were detected. RESULTS: Compared to control group, it was observed that there was mainly necrosis and partly degeneration in the renal tubular epithelial cells, proliferation of fibroblasts and infiltration of the inflammatory cells in the interstitial substance in gentamicin group under the light microscope. The renal indexes, serum creatinine, blood urea nitrogen, total urinary protein in 24 h, and the level of α-SMA, Smad2 were significantly higher than those in control group (P<0.01) and the level of Smad7 was significantly lower than that in control group (P<0.01). Under the light microscope, the results of all mentioned above in HYDZD+gentamicin group were improved significantly (P<0.01), the level of α-SMA, Smad2 were significantly lower than those in gentamicin group (P<0.01). However, no significant difference of Smad7 protein expression between gentamicin group and HYDZD+gentamicin group was observed (P>0.05). CONCLUSION: HYDZD is very effective in preventing and treating renal interstitial fibrosis caused by gentamicin with the decreased level of Smad2. 相似文献
18.
AIM: To investigate the effect of catalpol on the activity of osteoblasts (OB) and osteoclasts (OC), and OB estrogen receptor (ER) α/β mRNA expression in the OB-OC co-culture system. METHODS: OB and OC were isolated from the SD rats of 1 and 5 days old. In the OB-OC co-culture system, different concentrations of catalpol including low dosage (0.05, 0.1, 0.5 and 1 mg/L), middle dosage (2, 5 and 10 mg/L), and high dosage (20, 50 and 100 mg/L) were added into the culture medium to detect the changes of OB proliferation by MTT assay. The catalpol at maximal dosage was added to OB section to detect the alkaline phosphatase (ALP) activity of OB by pNPP method. The mRNA expression of ERα/β in the OB treated with catalpol in the co-culture system was detected by RT-PCR. The catalpol at maximal dosage was added to OC group to detect the activity of OC by microscopy and tartrate-resistantacid phosphatase (TRAP) activity detection. RESULTS: In 0.05~2 mg/L catalpol groups, the proliferation of OB was significantly increased as compared with control group in the co-culture system, and it reached the maximum value when catalpol was at 0.05 mg/L, while in 5~100 mg/L catalpol groups, the proliferation of OB was not increased. The ALP activity of OB in 0.05 mg/L catalpol group was higher than that in control group. The catalpal at 0.05 mg/L promoted the mRNA expression of ERβ in OB in the co-culture system, but did not increase the mRNA expression of ERα as compared with control group. Catalpol at 0.05 mg/L obviously inhibited the bone resorption and the TRAP activity in OC. CONCLUSION: Catalpol stimulates the proliferation and activity of OB, inhibits the bone resorption and activity of OC, and increases the mRNA expression of ERβ in OB in the OB-OC co-culture system, suggesting that high mRNA expression of ERβ may be the regulatory pathway of catalpol in response to bone metabolism. 相似文献
19.
AIM: To explore the influence of IFN-γ on the role of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in inducing apoptosis of RKO cell line. METHODS: Survival fraction and apoptosis were measured by MTT method and flow cytometry (FACS). RESULTS: Survival fraction of IFN-γ group, TRAIL and IFN-γ 72 h+TRAIL group were 99.28%, 85.45%, 52.60%, respectively. The percentage of apoptotic cells of IFN-γ group, TRAIL group, IFN-γ 24 h+TRAIL group, IFN-γ 48 h+TRAIL and IFN-γ 72 h+TRAIL group were 1.51%, 2.38%, 4.97%, 13.30%, 21.00%, respectively. The percentage of apoptotic cells of IFN-γ 24 h+TRAIL group was higher than the sum of IFN-γ group and TRAIL group (P<0.01). The longer the IFN-γ pretreated, the higher the percentage of apoptotic cells was observed (P<0.01). CONCLUSION: IFN-γ can reinforce the effect of TRAIL inducing apoptosis in RKO. 相似文献
20.
AIM: To investigate the antagonistic and protective mechanism of salviae miltiorrhizae injection(SMI) on kidney toxicity damaged by streptomycin (SM). METHODS: 40 guined pigs were divided randomly into 4 groups: the control group, SM group, SMI+SM group and SMI group. Light microscope, transmission electron microscope (TEM), immunohistochemical staining, TdT mediated biotin dUTP nick-end cabelling (TUNEL) techniques and image quantitative analysis technique were used to observe the change of kidney after administration of SM and SMI. The expressions of iNOS and bFGF, apoptosis, and the activity of N-acetyl-D-glucosaminidase(NAG) were also observed. RESULTS: 10 d after administration of SM, the serious pathological change in SM group was observed under the light microscope and transmission electron microscope. Slight damage was found in SMI+SM group, and the number of apoptosis cells was fewer than that in SM group. The result of immunohistochemical implied that the iNOS expression in SM group was higher than that in SMI+SM group, while the bFGF expression was lower. The activity of NAG in SMI+SM group was lower than that in SM group (P<0.01). CONCLUSION: Salvia miltiorrhiza injection inhibits the increased expression of iNOS during kidney toxicity damaged by SM, reduces the activity of NAG, increases the expression of bFGF, and decreases the apoptosis, hence has the antagonism to the kidney toxicity damaged by SM. 相似文献