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1.
AIM: To investigate the effect of cell cycle regulator p231WAF1 on hypertrophy of peritoneal mesothelial cells affected by high concentrated glucose. METHODS: RT-PCR and Western Blot method were used to detect p21WAF1 expression of rat peritoneal mesothelial cells in high glucose concentration medium (containing 1.86%, 3.86% glucose) after 24 hours. Flow cytometer technique was used to analyze the cell cycle distribution. RESULTS: In high glucose medium, most of the cells became hypertrophy, and were arrested in G1 phase of the cell cycle, which was obvious in 3.86% glucose group. Glucose increased p21 mRNA and protein expression in a dose-dependent manner, and the levels of p21WAF1 mRNA and protein in 3.86% glucose group were higher than those in 1.38% glucose group (P＜0.05). p21 WAF1 mRNA and protein expression were absent in the serum-free normal medium and D-mannitol groups which had the same osmolarity as the glucose groups. CONCLUSION: p21WAF1 may be pivotal in the hypertrophy and arrest in the G1 phase of mesothelial cells induced by high concentrated glucose.  相似文献   

2.
ZHANG Tuan-jie  REN Min 《园艺学报》2018,34(11):2096-2100
AIM: To evaluate the expression of Wnt/β-catenin signaling pathway-related proteins in breast cancer and the significance. METHODS: The patients with breast cancer (n=150) in our hospital from January 2015 to January 2017 were selected as study object. The tumor tissue samples of these patients were obtained from paraffin section of breast cancer by surgical resection with complete clinicopathological data. The corresponding paracancerous tissue sam-ples were taken from the non-tumor tissue samples from the above breast cancer patients, which were 0.5~1 cm away from the tumor tissue. The methods of real-time PCR and Western blot were performed to examine the expression of Wnt-1 and β-catenin at mRNA and protein levels. Human breat cancer MCF-7 cells were divided into 3 groups:control group (MCF-7 cells without treatment), agonist group[MCF-7 cells+Wnt3a (1 mg/L)] and antagonit group[MCF-7 cells+DKK1 (16 μmol/L)]. The expression of Wnt-1 and β-catenin at mRNA and protein levels was detected by real-time PCR and Western blot. RESULTS: Compared with the paracancerous tissues, the expression levels of Wnt-1 and β-catenin were higher in tumor tissues at mRNA and proteins levels (P<0.05). Notably, the positive expression rates of Wnt-1 and β-catenin were significantly higher in tumor tissues than that in the paracancerous tissues. Furthermore, Wnt-1 expression was associated with tumor metastasis (χ2=5.352, P=0.021), tumor stage (χ2=9.412, P=0.002) and tumor size (χ2=9.412, P=0.002). In addition, β-catenin expression was also associated with tumor metastasis (χ2=9.851, P=0.002) and tumor stage (χ2=5.661, P=0.017). Compared with control group, the expression of Wnt-1 and β-catenin at mRNA and protein levels in agonist group was increased (P<0.05),while that in antagonist group was decreased (P<0.05). CONCLUSION: The expression levels of Wnt-1 and β-catenin related with Wnt/β-catenin signaling pathway are increased in the breast cancer, which are closely related to the malignant state of the tumor.  相似文献   

3.
AIM: To study the role and regulation of calcineurin(CaN) in angiotensin II(AngⅡ)-stimulated cardiacmyocyte hypertrophy of rats. METHODS: Using AngⅡ to induce the cultured cardiac myocyte hypertrophy of rats, and investigating the effect of CaN inhibitor on [3H]-leucine incorporation of AngⅡ-stimulated cardiomyocytes and the regulation of various factors on CaN activity in cardiomyocytes.RESULTS: AngⅡ can stimulate the CaN activity in cultured neonatal rat cardiomyocytes in a dose- and time-dependent manner. In cardiac myocytes incubated with 10, 100, 1000 nmol·L-1 of AngⅡ for 12h, the CaN activities increased respectively by 13%,57%(P＜0.05) and 228%(P＜0.01) compared with that in non-stimulated cardiomyocytes. The CaN activities in AngⅡ-stimulated cardiomyocytes were significantly inhibited by losartan(50 μmol·L-1), H7(50 μmol·L-1)and Fura-2/AM(4 μmol·L-1),while no effect was observed with PD98059(50 μmol·L-1).The [3H]-leucine incorporation in AngⅡ-stimulated cardiomyocytes increased by 46%(P＜0.01) compared with that in control group, which was dramatically inhibited by cyclosporin A(0.5~5μg/mL). CONCLUSIONS: Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, may play an important role in AngⅡ-induced cardiac myocyte hypertrophy. The activation of CaN may dependent on the sustained increases of [Ca2+]i and be regulated by some protein kinases (such as PKC,etc.).  相似文献   

4.
AIM:To observe the effect of adrenomedullin(ADM)on proliferation of vascular smooth muscle cells(VSMC) induced by urotensin Ⅱ(UⅡ). METHODS:DNA synthesis of cultured rat aortic VSMC was measured by [3H]-TdR incorporation. The activities of mitogen activated protein kinase(MAPK) were determined by isotope tagged with [γ-32P]-ATP. RESULTS:UⅡ(10-8mol/L) significantly increased [3H]-TdR incorporation of VSMC and MAPK activities by 38%(P＜0.05) and 260%(P＜0.01) respectively compared with control group. Compared with UⅡ group, 10-10,10-9,10-8mol/L ADM decreased [3H]-TdR incorporation of VSMC by 7%(P＞0.05), 32%(P＜0.05)and 41%(P＜0.01),respectively, and diminished MAPK activities by 24%(P＞0.05), 32%(P＜0.05)and 36%(P＜0.05),respectively. CONCLUSION:ADM inhibits proliferation of VSMC induced by urotensin Ⅱ through inhibiting MAPK activation.  相似文献   

5.
AIM: To investigate the effect of Salidroside on the proliferation, DNA synthesis, intracellular Ca2+ content of rabbit PASMC (pulmonary artery smooth muscle cells) under hypoxia. METHODS: Techniques of cell culture, MTT test, [3H][3H][3H]-TdR incorporation, fluo-3 and confocal laser scanning microscopy were used. RESULTS: The A value of MTT and [3H][3H]-TdR incorporation of PASMC increased significantly by 62% (P＜0.05) and 138% (P＜0.01) after 24 h hypoxia. Salidroside (32×10-5 mol/L) inhibited the action of hypoxia on the proliferation of PASMC, the A value of MTT and [3H][3H]-TdR incorporation declined significantly by 29% (P＜0.05) and 37% (P＜0.01) compared with hypoxia group. A calcium channel blocker, verapamil could also inhibit the accelerative effect of hypoxia on the proliferation of PASMC. The intracelluler Ca2+ content of PASMC raised markedly under hypoxia, but the effect of hypoxia on the intracelluler Ca2+ content could be inhibited by Salidroside. CONCLUSION: Salidroside inhibited the proliferation, DNA synthesis of PASMC induced by hypoxia. The inhibitory action of Salidroside on the increase in intracellular Ca2+ concentration under hypoxia might be one of the mechenisms.  相似文献   

6.
AIM:To investigate the significance and changes of p14ARF gene in gastric cancer.METHODS:The tumors and gastric tissues neighboring carcinoma from 48 patients with gastric cancer were studied. The homozygous deletions, mutations, methylation of the CpG islands, and mRNA expression of p14ARF gene were assessed by PCR, PCR-SSCP, PCR based methylation assay, and RT-PCR.RESULTS:①The homozygous deletion rate of p14ARF was 31.3% (15/48), and no homozygous deletions were examined in all the gastric tissues neighboring tumor. ②There were no point mutations of p14ARF in 33 gastric cancers without homozygous deletion and in the matched gastric tissues adjacent to tumor. ③Methylation rate of the CpG islands of p14ARF was significantly higher in gastric cancers(47.9%, 23/48) than that in gastric tissues neighboring cancer (4.2%, 2/48)(P＜0.01).④ No expression of p14ARF mRNA was detected in 45.8%(22/48) of gastric cancers. Moreover, the negative rate (100%, 3/3) of p14ARF mRNA of gastric cancers with the combined methylation of exons 1β and 2 was significantly higher than that (15%, 3/20) of the sole methylation of exon2(P＜0.05). CONCLUSION:p14ARF gene is frequently inactivated by homozygous deletion and methylation of the 5' CpG islands in gastric cancer, which may play an important role in the development of gastric cancer.  相似文献   

7.
番茄绿果与红果颜色性状遗传的研究   总被引：3，自引：0，他引：3  
吴浪  刘婧仪  梁燕 《园艺学报》2016,43(4):674-682
以绿果番茄‘绿樱’和红果番茄‘TTD1003A’为亲本材料,构建4个世代P_1、P_2、F_1和F_2遗传群体,采用标准比色卡,对成熟果实的果色、果皮色、果肉色和胎座胶状物质颜色进行观察分析。结果表明:在F_2代分离群体中,果色分离比例为,红︰棕︰黄︰绿=9︰3︰3︰1;果皮色为,黄色︰透明=3︰1;果肉色为,红︰浅黄︰浅绿=12︰3︰1,即果色、果皮色和果肉色的遗传符合孟德尔遗传规律,且分别由两对、一对和两对核基因控制;果实绿色相对果实红色为隐性,果皮透明相对果皮黄色为隐性,果肉浅绿色相对果肉红色为隐性,果皮与果肉颜色独立遗传。同时,运用色差仪测定果实表面颜色的L值、a值和b值,计算色光值后,运用植物数量性状主基因+多基因遗传分析法分析得出:番茄果实绿色对红色的遗传可能符合两对加性—显性—上位性主基因+加性—显性多基因遗传(MX2-ADI-AD),其中两对主基因均以加性效应为主,第一对主基因的加性作用更为明显。在F_2代中,色差仪测定指标的主基因遗传率为76%~89%,而多基因遗传率接近0,即该组合控制果色性状的主基因遗传力很高,多基因遗传力很低,对番茄果色的选择应在分离早期世代进行。  相似文献   

8.
AIM: To investigate the role of Th17 cells in the patients with cervical cancer.METHODS: We measured the peripheral levels of Th17 cells and CD3+CD8-IL-21+ T cells in 37 cervical cancer (CC) patients, 25 cervical intraepithelial neoplasia (CIN) patients and 18 healthy controls by flow cytometry. The percentages of Th17 cells and CD3+CD8-IL-21+ T cells in total CD4+ cells were calculated.RESULTS: Compared with controls, the patients with CC or CIN had higher proportions of Th17 cells (all P<0.01) and CD3+CD8-IL-21+ T cells (all P<0.05). Notably, in CC patients, the increased percentages of Th17 cells and CD3+CD8-IL-21+ T cells were independently associated with the clinical stage(all P<0.05), lymph node metastasis (P<0.01,P<0.05) and vasoinvasion (all P<0.01), while the elevated percentage of CD3+CD8-IL-21+ T cells was also associated with the tumor size(P<0.01). Furthermore, the percentage of Th17 cells was positively correlated with that of CD3+CD8-IL-21+ T cells in healthy controls and CC patients, but not in CIN patients.CONCLUSION: Our results indicates a possible role of Th17 cells in CC patients correlated with CD3+CD8-IL-21+T cells, and the elevated percentage of circulating Th17 cells may be involved in the development and progression of cervical cancer.  相似文献   

9.
AIM: To investigate the correlation of UGT1A1 *28 and UGT1A1 *6 gene polymorphisms with irinotecan-associated adverse events and efficacy in the patients with metastatic colorectal cancer (mCRC) treated with irinotecan-based chemotherapy. METHODS: Analysis of UGT1A1 *28 and UGT1A1 *6 gene polymorphisms was performed in 207 gastrointestinal cancer patients admitted to our hospital from April 2010 to March 2012 by amplifying the gene fragments using PCR and direct sequencing. Fifty six cases with mCRC treated with irinotecan were chosen to observe the adverse events and efficacy during chemotherapy, and the time to progression (TTP) was also recorded. The incidence of different genotypes was compared. RESULTS: The distribution of the genotypes in 207 gastrointestinal cancer patients was as follows: UGT1A1 *28 wild-type (WT) genotype TA6/6 (164, 79.2%), heterozygous genotype TA6/7 (41, 19.8%), and homozygous genotype TA7/7 (2, 1.0%); UGT1A1 *6 WT genotype G/G (154, 74.4%), heterozygous genotype G/A (51, 24.6%), and homozygous genotype A/A (2, 1.0%). In the 56 mCRC cases, the incidence of grade 3 and 4 delayed diarrhea and neutropenia in the patients carrying UGT1A1 *6 (G/A and A/A) was higher than that in the WT genotype (6/6) (38.9% vs 7.9%,61.1% vs 29.0%, both P<0.05). The incidence of grade 3 and 4 thrombocytopenia in the patients carrying UGT1A1 *28 (TA6/7 and TA7/7) was higher than that in the WT genotype (TA6/6) (33.3% vs 2.1%, P<0.05). No significant difference of TTP and chemotherapeutic effect was observed between different genotypes. CONCLUSION: The UGT1A1 *6 (G/A and A/A) genotypes increase the risk of grade 3 and 4 delayed diarrhea and neutropenia, and the UGT1A1 *28 (TA6/7 and TA7/7) genotypes increase the risk of grade 3 and 4 thrombocytopenia in mCRC patients treated with irinotecan-based chemotherapy.  相似文献   

10.
AIM: To Compare immunogenicity of three kinds of heterogenic corneal stroma. METHODS: 36 SD rats were randomized into 4 groups, each group consisting of 9 rats. Group 1 was control group. Three kinds of heterogenic corneal stroma: porcine, rabbit and chicken corneal stroma were heterotopically transplanted to subcutaneous layer of 27 (group 2-4) SD rats, respectively. The expression of CD4+, CD8+, CD25+, CD71+ on peripheral T cells was identified and analyzed by dual fluorescence flow cytometry at 7, 14, 28 days after operation. RESULTS: Compared with control group, the expression of CD4+, CD8+, CD25+, CD71+ was no significant change in porcine corneal stroma group(P＞0.05), the expression of CD4+ was increased in rabbit corneal stroma (P＜0.05), CD4+, CD4+ CD71+ markedly higher in the chicken corneal stroma (P＜0.01) at 7 days after operation. CONCLUSION: The immunogenicity of porcine stroma is the lowest in three kinds of heterogenic corneal stroma (chicken, rabbit and porcine).  相似文献   

11.
AIM: To investigate the expression and location of SET domain-containing 4(SETD4) protein in p38 +/+ and p38 -/- cells treated with sodium arsenite(NaAsO2). METHODS: The expression and location of SETD4 were detected in different cells with or without NaAsO2 treatment by Western blotting and immunofluorescence technique. RESULTS: The expression of SETD4 was detectable in both murine- and human-derived cells. Its distribution was found to be located in the whole cell, mainly in the cytoplasm. Further investigation also suggested that the protein expression of SETD4 was reduced in both p38 +/+ and p38 -/- cells 6 h after NaAsO2 treatment. Moreover, SETD4 protein was translocated from cytoplasm to nucleus in p38 +/+ cells treated with NaAsO2, which was unobvious in p38 -/- cells. CONCLUSION: SETD4 protein is expressed in various cells derived from different species and tissues, and it is mainly located in cytoplasm. NaAsO2 treatment influences the expression of SETD4, and induces the translocation of SETD4 protein to nucleus, which might be involved in the p38 MAPK signal pathway.  相似文献   

12.
AIM: To explore the ex vivo expansion characteristics of the endothelial progenitor cells (EPCs). METHODS: CD34+ cells were selected from umbilical cord blood mononuclear cells (MNC) by MiniMACS system, expanded at the same conditions as that for total MNC, coincubation of CD34+ and CD34- from the same donation for EPCs. In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis. EPCs were determined and quantified by immunocytochemistry and flow cytometry. RESULTS: Coculture of CD34+ and CD34-,total MNC led to a significant increase in the expansion of CD34+ cells compared with CD34 enrichment (P＜0.05). There was a trend toward decreased apoptosis in cultures when early passage was performed once the linear cord like structures appeared. There was no significant effect on apoptosis between with VEGF and without VEGF group (P＞0.05). These differentiated EPCs were stained positive for CD34+, von Willebrand factor (vWF), KDR, CD31 and incorporate acetylated low-density lipoprotein (LDL). CD34+ and AC133+cells accounted for 68.2%±6.3% (n=6) and 57.2%±9.8% (n=6) of attaching (AT) cells at day 7 of culture, respectively. CONCLUSIONS: Coculture of CD34+ and CD34- or culture of MNC enhances ex vivo expansion of EPCs. Early passage decreases apoptosis rate, VEGF has no significant effect on ex vivo expansion of EPCs.  相似文献   

13.
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14.
AIM: To investigate the clinical significance of the new method of modified immunomagnetic enrichment of tumor cells in combination with fluorescent immunocytochemistry to detect circulating tumor cells (CTCs) in peripheral blood samples of patients with nasopharyngeal cancer (NPC). METHODS: Peripheral blood samples were obtained from 76 histology-proven patients with NPC before the initial therapy. After isolation of the mononuclear cells, the CTCs expressing cytokeratin (CK)8/18 in the blood samples were detected by the method of immunomagnetic enrichment in combination with fluorescent immunocytochemistry. The magnetic beads covalent binding with epithelial cell adhesion molecule (EpCAM) antibody were used to enrich the tumor cells which expressed EpCAM. After median following-up for 25 months, the effects of CTCs and other prognostic factors on patient prognosis were thoroughly investigated. RESULTS: None of the positive CK8/18 cells was detected in 20 normal blood samples. The CTCs were detected in 82.9% of the patients (P<0.01). Relapse patients had significantly higher number of median CK8/18+ CTCs than the patients without relapse (P<0.01). No association of viral capsid antigen (VCA)-IgA (P>0.05) was observed between the patients with and without relapse. Relapse-free survival rates were lower when the number of peripheral blood CK8/18+ CTCs was more than 3. The 2-year relapse-free survival rates were 100%, 100%, 100%, 94.1%, 71.4%, 53.3% and 44.4%(P<0.01) when the numbers of peripheral blood CK8/18+ CTCs were 0, 1, 2, 3, 4, 5, 6 before treatment, respectively. Overall survival rates were lower when the number of peripheral blood CK8/18+ CTCs was more than 5, but the difference was not significant. The 2-year overall survival rates were 100%, 100%, 100%, 100%, 100%, 100%, 80% and 77.8% (P>0.05) when the numbers of peripheral blood CK8/18+ CTCs were 0, 1, 2, 3, 4, 5, 6 before treatment,respectively. The VCA-IgA titer could not predict survivals. Cox multivariate analysis also showed the same results. CONCLUSION: Peripheral blood CK8/18+ CTCs are a prognostic factor for initial treatment of NPC.  相似文献   

15.
AIM:To observe the changes of iNOS and eNOS in lung tissue and NO in bronchoalveolar lavage fluid (BALF) in smoking rats.METHODS:80 Wistar rats were divided into control, smoking group, L-NIL group and L-NAME group (rats were exposed to smoke and injected (i.p.) with selective iNOS inhibitor L-NIL or NOS inhibitor L-NAME). iNOS and eNOS protein levels in whole lung were detected by immunohistochemical staining, and NOS mRNA was quantified using RT-PCR. In addition, NO2-/NO3- was determined using Griess assay.RESULTS:The expression of iNOS mRNA and protein in smoking rats increased, the expression of eNOS mRNA and eNOS protein decreased, and the total cell count and the level of NO2-/NO3-in BALF increased(P＜0.05). In vivo, L-NIL reduced the total cell count and NO2-/NO3- in BALF (P＜0.05), while L-NAME had no effect on them.CONCLUSION:Cigarette smoke increased expression of iNOS mRNA and protein and decreased expression of eNOS mRNA and protein. The large amount of NO generated by iNOS may amplify inflammation in lung tissue.  相似文献   

16.
AIM: To investigate the expression of CUG-binding protein 1 (CUGBP1) in breast cancer tissues, and to explore the effect of CUGBP1 gene silencing on the viability and invasion ability of human breast cancer MCF-7 cells. METHODS: A total of 96 cases of patients with breast cancer undergoing surgical treatment were selected in the Second Affiliated Hospital of Zhengzhou University from March 2015 to September 2017. Immunohistochemical staining was used to detect the protein expression of CUGBP1 in the breast cancer and adjacent tissues. MCF-7 cells were cultured and divided into CUGBP1 interference sequence group, control sequence group and blank group. Western blot was used to detect the protein expression of CUGBP1, Twist, E-cadherin and vimentin in the cells. The cell viability was measured by MTT assay. The cell invasion ability was detected by Transwell assay. RESULTS: The positive expression rate of CUGBP1 protein in the breast cancer tissues was higher than that in the adjacent tissues (χ2=28.900, P<0.001). The differences of CUGBP1 protein expression in the breast cancer tissues among TNM staging, histological grading and lymph node metastasis were statistically significant (P<0.05). The relative protein expression levels of CUGBP1, Twist and vimentin in CUGBP1 interference sequence group were lower than those in control sequence group and blank group, while the relative protein expression of E-cadherin was higher than that in control sequence group and blank group (P<0.05). The cell viability at 24 h, 48 h, 72 h and 96 h in CUGBP1 interference sequence group was lower than that in control sequence group and blank group (〖P<0.05). The invasive cells in CUGBP1 interference sequence group were less than those in control sequence group and blank group (P<0.05). CONCLUSION: CUGBP1 protein is highly expressed in the breast cancer tissues. Specific silencing of 〖STBX〗CUGBP1〖STBZ〗 gene expression in breast cancer MCF-7 cells effectively inhibits the cell viability and invasiveness, and its mechanism may be related to inhibiting the process of epithelial-mesenchymal transition.  相似文献   

17.
AIM:To investigate the effect of cyclosporine A on rat cardiomyocyte hypertrophy caused by neuropeptide Y (NPY).METHODS:Cardiomyocytes of neonatal Wistar rats were cultured with NPY or NPY together with CsA. For assessing protein synthesis rate and c-jun mRNA expression in cardiomyocytes, the methods of [3H]-Leu incorporation and RT-PCR were used.RESULTS:(1) [3H]-Leu incorporation in cardiomyocytes: [3H]-Leu incorporation in NPY (10 nmol/L) group was higher than that in control group, but there were no distinct changes between two groups. To compare with control group, [3H]-Leu incorporation in NPY (100 nmol/L) group were increased significantly (P＜0.05). There was no significant change between control group and CsA group; (2) c-jun mRNA expression in cardiomyocytes: RT-PCR production of c-jun mRNA in NPY group was enhanced considerably compared with CsA group and control group (P＜0.01). There was no significant change between CsA group and control group.CONCLUSIONS:NPY can induce cardiomyocyte hypertrophy. Cyclosporine A (inhibitor of CaN) can blunt the effect of NPY, suggesting that the Ca2+/CaM-dependent calcineurin (CaN) signaling pathway plays an important role in it.  相似文献   

18.
AIM: To study the expression of glypican-3 in hepatocellular carcinoma (HCC) tissues and to clarify its clinical significance. METHODS: The expression of GPC3 was detected in 59 cases of HCC and their para-cancerous tissues, 10 cases of intrahepatic cholangiocellular carcinoma (ICC), 11 cases of cirrhotic tissues and 14 cases of normal liver tissues (around haemangioma) by RT-PCR and immunohistochemical staining. The survival curves were constructed using Kaplan-Meier method and evaluated using the log-rank test. In addition, the Cox proportional hazards regression model was established to identify the factors that were independently associated with disease-free survival (DFS). RESULTS: The mRNA expression of GPC3 in the HCC tissues was significantly higher than that in the para-cancerous tissues (83.1% vs 35.6%, χ2=27.53, P<0.01). The protein expression of GPC3 in the HCC tissue was also higher than that in the para-cancerous tissues (78.0% vs 33.2%, χ2=24.97, P<0.01). The expression of GPC3 in ICC tissues, liver cirrhosis tissues and normal liver tissues was undetectable. Kaplan-Meier survival analysis indicated that the GPC3(+)HCC patients had worse 1-year DFS than that of GPC3(-) patients (33.6% vs 72.7%, P<0.05). The HCC patients with para-cancerous GPC3(+) also had worse 1-year DFS than that of the para-cancerous GPC3(-) patients (23.5% vs 40.1%, P<0.05). The DFS rate decreased significantly as the expression intensity of GPC3 increased. The Cox regression model analysis indicated that AFP(+) (odd ratio=0.372, 95% confidence interval: 0.140-0.900, P<0.05), tumor size (odd ratio=5.215, 95% confidence interval: 1.737-15.656, P<0.01), para-cancerous tissue GPC3(+) (odd ratio=0.226, 95% confidence interval: 0.085-0.599, P<0.01) and the intensity of GPC3 expression in HCC tissue (odd ratio=1.946, 95% confidence interval: 1.080-3.507, P<0.05) were the independent risk factors linked to DFS of patients. CONCLUSION: GPC3 protein is highly expressed in the HCC tissues,but not in ICC, cirrhotic liver and normal liver tissues. The expression of GPC3 in para-cancerous tissues and the intensity of GPC3 expression in HCC tissues are the important independent risk factors linked to DFS of patients.  相似文献   

19.
AIM: To characterize the proportion of CD14+CD16+ monocytes in peripheral blood from type 2 diabetes (T2DM) patients and to observe the response of CD14+CD16+ monocytes to lipopolysaccharide (LPS) and interleukin-15 (IL-15) for further exploring the potential mechanism of inflammatory immune response in the pathogenesis of T2DM. METHODS: Twenty-eight patients with T2DM and 20 healthy volunteers were enrolled in the study. The peripheral blood was collected for determining the percentage of CD14+CD16+ monocytes by flow cytometry. The peripheral blood mononuclear cells (PBMC) were isolated and subject to stimulation with LPS and IL-15 for 4 h. The protein expression of STAT5 was detected by Western blotting and the phosphorylated (p)-STAT5 was determined by Western blotting and immunofluorescence. Serum levels of 25-hydroxyvitamin D3 and IL-6, and the concentrations of IL-6 and monocyte chemoattractant protein-1(MCP-1) in the culture supernatants were assessed by ELISA. Serum level of C-reactive protein (CRP) was measured by immunoturbidimetry. RESULTS: There were positive correlations between the quantity of CD14+CD16+ monocytes and serum levels of CRP and IL-6 (r=0.394, P<0.05 and r=0.741, P<0.01), while serum 25 (OH) D3 was negatively correlated with the quantity of CD14+CD16+ monocytes (r=-0.409, P<0.01), serum CRP(r=-0.479,P<0.01) and serum IL-6 (r=-0.774,P <0.01). After stimulated with LPS and IL-15, PBMC showed significant up-regulation of p-STAT5 protein expression, and significant increases in the supernatant levels of IL-6 and MCP-1 were observed (P<0.05). The expression of p-STAT5 existed in the nucleus.CONCLUSION: These findings suggest that the functional disturbance in monocytes occurs in T2DM, which may be related to insufficiency of vitamin D3. The aberrant activation of STAT5 signaling pathway underlies the functional abnormalities of the monocytes in T2DM.  相似文献   

20.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.  相似文献   

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