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1.
AIM: To investigate the variation and distribution of abnormaly methylated p15 INK4B in myelodysplastic syndrome (MDS) and its subgroups. METHODS: The abnormal methylation of p15 INK4B in 32 cases with MDS was studied using methylation-specific PCR (MSP). RESULTS:The positive rate of abnormal methylation of p15 INK4B was about 50% in MDS. The ratios in subtypes were: 0% (0/6) in RA,20% (1/5) in RA-S,57.1% (4/7) in RAEB,74.1% (5/7) in CMML,85.7% (6/7) in RAEB-t, respectively.It was worth noticing that 4 cases represented abnormal methylation of p15INK4B during their transformation and progression into AML. CONCLUSION:The abnormal methylation in p15 INK4B might be one of the causes of MDS, which was related to pathologial process of MDS.Every subtype was not solitary classification completely. Abnormal methylation of p15 INK4B was apt to occur accompanying the progression and transformation of the subtypes.  相似文献   

2.
AIM: To study the characteristics and regulations of p15 (MST INK4B) methylation in multiple myeloma (MM). METHODS: Method of methylation-specific PCR was applied in 23 cases of MM about the methylation rate of p15 gene. RESULTS: Methyaltion rate in MM was 73.5%(17/23). The PCR product was a fragment of 148 bp. In four stageⅠcases of MM with plasma cell-typed bone marrow profile, p15INK4B gene was non-methylated; In one case of stag ⅡMM and one case of stage Ⅲ MM with mature plasma typed bone marrow profile, p15INK4B gene was no-methylated, too, while in many cases of stageⅠ、stage Ⅱand stage Ⅲ with naive plasma cell in bone marrow profile which were plasma cell-typed or mixed typed, p15INK4B gene methylation was frequently detected. The methylation rates for stageⅠ、stage Ⅱand stage Ⅲ MM were respectively 55%(5/9),100%(7/7) and 71.4%(5/7). CONCLUSION: p15 gene methylation was a possible pathogenic factor,and might be related to the progression and prognosis of the disease.  相似文献   

3.
AIM:To study the effect of p19ARF on the biological behavior of human leukemia cells. METHODS:p19ARF was cloned in eukaryotic expression vector pcDNA3.1 and transferred into INK4a/ARF locus-deficient leukemia cells HEL and K562. The changes in biological characteristics of the two p19ARF-transfected cells were observed.RESULTS:The growth of the p19ARF-transfected HEL cells was significantly inhibited compared with the vector-transfected cells; Cell cycle analysis showed that the expression of foreign p19ARF gene resulted in G1 phase cell cycle arrest and apoptosis cell death in some of HEL cells. However, p19ARF had no marked effects on the growth of K562 cells with p53 gene mutation and did not induce apoptosis in K562 cells.CONCLUSION:p19ARF suppressed the growth of leukemia cells by p53-dependent pathway.  相似文献   

4.
AIM: To investigate the characteristic of T-cell acute lymphocytic leukemia 1 (TAL1) gene expression in acute myeloid leukemia (AML) cell lines and in primary AML cells from de novo AML patients with different subtypes. METHODS: Real-time PCR was used to determine the expression of TAL1 mRNA in acute leukemia cell lines (Jurkat, CCRF-CEM, HL-60 and NB4 cell lines) and peripheral blood mononuclear cells from 47 newly diagnosed AML patients. Twelve healthy individuals were served as healthy control group. RESULTS: A significantly increased level in TAL1 mRNA was found in AML cell lines (HL-60 and NB4), T-cell acute lymphacytic leukemia (T-ALL) cell lines (Jurkat, CCRF-CEM) and primary AML cells compared with the healthy controls. Over-expression of TAL1 was found in all detected AML subtypes, the highest level of TAL-1 mRNA was found in AML-M1 and AML-M5 subtype (P<0.05). CONCLUSION: High expression of TAL1 in AML might influence the differentiation and proliferation of myeloid cells, further investigation needs to confirm whether it would be as a biomarker for pathogenesis of AML.  相似文献   

5.
AIM: To investigate the relationship between promoter hypermethylation of secreted frizzled-related protein (SFRP) genes and acute leukemia (AL)，and to study the mechanism how 5-aza-2-deoxycytidine (5-Aza-CdR) reverses the hypermethylation of SFRP genes in human AL cell lines. METHODS：Methylation-specific PCR (MSP) was used to detect the methylation levels of SFRP1, SFRP2, SFRP4 and SFRP5 genes in different human AL cell lines (Molt-4, Jurkat, HL-60 and NB4). The methylation levels of these genes in Jurkat cell line before and after 5-Aza-CdR treatment were also analyzed by MSP. The expression of SFRP1, SFRP2, SFRP4 and SFRP5 mRNA was detected by real-time fluorescence quantitative RT-PCR. The mRNA levels of DNA methyltransferase (DNMT) 1, DNMT3A and DNMT3B were analyzed by semiquantitative RT-PCR. RESULTS：None of normal human blood or bone marrow mononuclear cells showed methylation of SFRP genes. Hypermethylation in the promoter regions of SFRP1, SFRP2 and SFRP5 genes was observed in all of the four AL cell lines. SFRP4 gene was totally methylated in NB4, Molt-4 and Jurkat cell lines but partially methylated in HL60 cell line. Treatment with 5-Aza-CdR for 72 h successfully reversed the hypermethylation of SFRP genes, and significantly increased the mRNA expression of SFRP. Moreover, the mRNA expression of DNMT1, DNMT3A and DNMT3B was down-regulated by 5-Aza-CdR in a concentration-dependent manner. CONCLUSION：Methylated SFRP genes may serve as potential independent biomarkers for early detection of AL. 5-Aza-CdR activates SFRP gene expression by demethylation of SFRP genes and down-regulation of DNMT1, DNMT3A and DNMT3B mRNA expression.  相似文献   

6.
AIM: To define the gene expression changes of vascular smooth muscle cells (VSMCs) in response to norepinephrine (NE). METHODS: The expression adrenergic receptors (AR) were determined by radioligand binding assay in A7r5 cells. Gene expression profiles were identified by cDNA microarray after A7r5 cells were treated with NE for 24 h, and mRNA expressions of α1A-AR and α1B-AR were confirmed by real-time PCR. RESULTS: α1-AR and β-AR existed in A7r5 cells. Seventy-five genes with changed expression in response to NE were screened out. These genes are involved in cell structure, cell/organism defense, metabolism, signal transduction and so on. α1A-, α1B-AR mRNA expression identified by microarray and realtime quantitive PCR displayed similar patterns. CONCLUSIONS: Gene expression profile in response to NE was analyzed comprehensively with the microarray technique. NE induces many kinds of different function genes in A7r5 cells, which may provide a novel insight into the particular role of NE that modulates multiple aspects of biological function in VSMCs.  相似文献   

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9.
AIM: To investigate the significance of aberrant p53 gene promoter methylation in acute leukemia by detecting the occurrence of p53 gene promoter methylation. METHODS: Genomic DNA was digested using restriction endonuclease MspⅠ, HpaⅡ, EcoRⅡ, BstNⅠ, respectively. PCR amplification was conducted and the products after digestion and genomic DNA were used as template. The PCR product was subjected to electrophoresis and the results were analyzed by gel imaging and analysis system. Parts of the separated DNA were sequenced after purification from gel. RESULTS: The prevalence of methylation in acute leukemia group was 38.7％, of which ALL was 45.5％ (5 of 11) and ANLL 35.0％ (7 of 20). No methylation was detected in normal control group. There was significant difference between the prevalence of methylation in acute leukemia group and the normal control group (Fisher′s exact test, P<0.05). However, the prevalence between ALL and ANLL was not significantly different (Fisher′s exact test, P>0.05). Compared the relationship between aberrant methylation of p53 gene and clinical data, statistical significance between aberrant methylation of p53 gene and enlargement of lymph nodes, liver or spleen(P<0.05) was observed. CONCLUSION: ①Aberrant DNA methylation in P1 promoter region of p53 gene exits in part of acute leukemic patients, but not in health people. ②The prevalence of aberrant DNA methylation between ALL and ANLL is not significantly different. ③The patients with aberrant methylation of p53 gene seem to show more frequently the manifestations of enlarged lymph nodes, liver or spleen than usual.  相似文献   

10.
CAI Jiang-yi  ZHU Le-le 《园艺学报》2000,36(9):1602-1607
AIM To investigate the expression of histone chaperone anti-silencing function 1B （ASF1B） in prostate cancer cells and its effect on cell viability in vitro. METHODS Human prostate cancer PC-3 cells were used, and knockdown of ASF1B was conducted by small interfering RNA （siRNA） transfection into the cells. The cells were divided into control group, siRNA negative control vector （mock） group and siRNA-ASF1B group. The viability of the PC-3 cells treated with ASF1B-siRNA for 12, 24 and 48 h was measured by MTT assay. Flow cytometry was used to analyze cell apoptosis and cell cycle distribution. The mRNA expression of apoptosis-related molecules was detected by RT-qPCR, and the expression levels of MAPK/JNK/ERK signaling pathway-related proteins were determined by Western blot. RESULTS The protein level of ASF1B in the normal cells （benign prostatic hyperplasia） was significantly lower than that in the PC-3 cells （P<0.01）. Compared with control group and mock group, the protein expression level of ASF1B in the PC-3 cells transfected with siRNA-ASF1B plasmid and the viability of the PC-3 cells were significantly decreased （P<0.01）. The apoptotic rate of the PC-3 cells transfected with siRNA-ASF1B plasmid was significantly higher than that in control group （P<0.01）, and the cell cycle was arrested at G1 phase. The mRNA levels of p53, caspase-3, Bax and PARP-1 in the PC-3 cells transfected with siRNA-ASF1B plasmid were up-regulated compared with those in control group and Mack group （P<0.01）. In addition, the protein levels of MAP2K4 and p-JNK in the PC-3 cells in siRNA-ASF1B group were significantly higher than those in mock group （P<0.01）, while the protein level of p-ERK was significantly lower than that in mock group （P<0.01）. CONCLUSION ASF1B silencing induces G1 arrest and promotes apoptosis of PC-3 cells. Activating MAPK/JNK/ERK signaling pathway may be a possible contributor to the anti-prostate cancer effect of siRNA-ASF1B.  相似文献   

11.
AIM: To investigate nucleostemin gene expression in bone marrow of acute leukemia and its clinical significance.METHODS: The expression of nucleostemin in 67 acute leukemia patients was detected by fluorescent quantitative polymerase chain reaction (FQ-PCR). The correlation between the expression level of nucleostemin gene and clinical significance was analyzed.RESULTS: Significantly higher expression levels of nucleostemin gene were detected in the initially-treated acute leukemia patients than those in normal control group and complete remission (CR) group by FQ-PCR (P<0.01). The expression level of nucleostemin gene in the cells from ALL was significantly lower than that of the cells in ANLL (P<0.05). No significant difference of nucleostemin expression in various differentiation stages (M2, M3, M4, M5) of ANLL was found (P>0.05). No significant association was observed between nucleostemin expression levels and age, sex, hepatauxe, splenomegaly, WBC count of acute leukemia patients by logistic analysis. The patients with positive expression of nucleostemin had significantly lower complete remission rate than those with negative expression (51.3% vs 83.3%, P<0.05). The nucleostemin expression level was significantly reduced during complete remission. Long-term follow-up of nucleostemin expression level showed that continuous or significant increase in nucleostemin expression in acute leukemia patients predicts refractoriness and impending relapse.CONCLUSION: Expression level of nucleostemin in acute leukemia patients is obviously higher than that in normal control. Nucleostemin can be a marker for evaluating therapy efficacy and monitoring minimal residual diseases (MRD) in leukemias.  相似文献   

12.
AIM: To investigate the expression of cystic fibrosis transmembrane conductance regulator (CFTR) in acute myeloid leukemia (AML) and its effect on the biological function of human erythroleukemia cell line TF1, and to explore the underlying mechanism. METHODS: The abundance of CFTR in the bone marrow mononuclear cells of patients with AML was measured by real-time PCR. After TF1 cells were incubated with CFTR specific inhibitor CFTRinh-172, cell viability, cell cycle distribution and cell apoptosis were analyzed by CCK-8 assay and flow cytometry. The Wnt signaling pathway-related proteins were determined by Western blot. RESULTS: CFTR was highly expressed in both patients with AML and leukemia cell lines. After incubated with CFTRinh172, the viability of TF1 cells was decreased, the proportion of the cells in G0/G1 phase was increased, while that in S phase declined (P<0.05). Furthermore, the cells treated with CFTRinh-172 exhibited higher apoptotic rate, accompanied with lower protein expression of β-catenin, c-Myc and cyclin D1 (P<0.05). CONCLUSION: CFTR expression is dramatically increased in AML. Inhibition of CFTR restrains the growth and promotes the apoptosis of TF1 cells via classical Wnt signaling pathway.  相似文献   

13.
AIM and METHOD: The relationship between the evolution of the reticulin fibres(RF) or the collagen fibres(CF) and the growing of hematopoietic cells in long-term bone marrow culture (LTBMC) from 15 paticnts with acute myeloid leukemia (AML) and form 6 normal subjects was observed by inverted microscope, Gomoris staining and Massons staining were used. RESULTS: (1)The amount of RF contents of 8 AMLs, with self- maintained(AMLsm) in the 1~8 weeks-old-culture was significant less than that of normal control and 7 AMLs, without self-maintained(AMLnsm) (P＜0.05). On the contrary, the amount of CF coutents of AMLsm in the 1~6 weeks-old-culture was significant more than that of normal and AMLnsm(P＜0.05). (2) There were many hematopoietic cells in the areas of CF, and there were few in the areas of RF. (3)During the whole culture, the most part of confluence of the adherent layer only observed in 3 AMLsm. CONCLUSION: A proper amounts of fibres in the extracellular matrixes of bone marrow is necessary for hematopoiesis, but the change of the proportion of RF contents to CF contents may play an important role in the development of AML and other malignant blood diseases.  相似文献   

14.
QIAN Jun  CHEN Zi-xing 《园艺学报》2003,19(8):1137-1140
Myelodysplastic syndrome(MDS)is considered as a preleukemic course, characteristic of hypercel ular marrow and pancytopenia.Many studies have demonstrated that defects occur in the heamtopoietic cels from patients with MDS.Recently, many abnormal changes in apoptosis, proliferation, ability of hematopoietic support, cytokine secret ion, clonal origin of stromal cells and angiogenesis have also been re vealed in the bone marrow microenvironment of MDS patients.  相似文献   

15.
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16.
AIM: To investigate the expression of aplasia rashomolog member I (ARHI) gene in acute myeloid leukemia cells (AML) and to study the effects of ARHI on the growth of AML cell line U937.METHODS: The mRNA expression of ARHI in AML cells, 293FT cells, AML primary cells and healthy volunteer blood cells were detected by RT-PCR. After transfection with the MSCV-IRES-GFP-ARHI plasmid to the U937 cells, the growth curve was analyzed by MTT assay. U937 cells were re-suspended by fresh medium and cultured for 24 h, then the cell cycle distribution and apoptotic rate were determined. RESULTS: The mRNA of ARHI was positively detectable in 293FT cells and healthy volunteer blood cells instead of AML cell line and AML primary cells. The growth curve showed that cell viability in U937 cells with high expression of ARHI (U937-ARHI) was lower than that in the control cells (U937-GFP) on 6th~8th day. The ratio of G2/M phase and apoptotic rate in the U937-ARHI cells were increased compare with control group(P<0.05). CONCLUSION: The mRNA level of ARHI is low in AML cells. High expression of ARHI gene in U937 cells inhibits cell growth, arrests the cells at G2/M phase and induces apoptosis.  相似文献   

17.
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18.
AIM and METHODS: To investigate the expression of adhesion molecule β2 integrins (CD11a、CD11b) and L-selectin (CD62L )on acute lymophocyte leukemia (ALL) cells and its clinical implications. Adhesion molecules CD11a, CD11b and CD62L of 45 ALL patients and 25 health people were measured by flow-cytometric analysis. RESULTS: ①CD11a and CD11b expression were lower on ALL cells than the normal hematopoietic cells. The rate of low expression was 100% for CD11b, 50% for CD11a, respectively. CD62L expression were higher on ALL cells than the normal hematopoietic cells. ②The CD11a was lower expressed on B-ALL than T-ALL. CD62L was higher on T-ALL than B-ALL. ③The expression of CD11a in the invasion group was much higher than that in the non-invasive group (P<0.05). ④The levels of CD11a, CD11b were returned to normal levels at remission. CONCLUSION: These results suggest that there are abnormalities in the expression of cell adhesion molecules in ALL which may help identify ALL subtypes and the treatment effect.  相似文献   

19.
AIM: To investigate the expression of microRNA (miRNA)-93 in acute lymphocytic leukemia (ALL) and its effect on the proliferation of acute T-cell leukemia Jurkat cells.METHODS: The expression of miRNA-93 in the bone marrow samples of patients with ALL was measured by real-time PCR. After down-regulation of miRNA-93 by transfection with miRNA-93 inhibitor in the Jurkat cells, the cell viability, cell proliferation and cell cycle distribution were detected by CCK-8 assay, EdU assay and flow cytometry, respectively. Furthermore, the protein levels of cell cycle-related molecules such as cyclin D1, cyclin-dependent kinase 4 (CDK4), phosphorylation retinoblastoma (Rb) and P27 were measured by Western blot.RESULTS: miRNA-93 was highly expressed in the patients with ALL, and the expression level was highest in the high risk patients. Down-regulation of miRNA-93 inhibited Jurkat cell viability, arrested cell cycle in G1/S transition. In addition, the protein levels of cyclin D1, CDK4 and p-Rb were significantly decreased, the protein expression of P27 was increased in Jurkat cells trasfected with miRNA-93 inhibitor.CONCLUSION: miRNA-93 expression is increased in ALL patients. Down-regulation of miRNA-93 restrains cell proliferation in the acute T cell leukemia cell line Jurkat via regulating cell cycle-related molecules.  相似文献   

20.
AIM: To observe whether transfection of mammalian expression vector pEGFP containing the gene of B-cell specific moloney leukemia virus insertion site 1(BMI-1) could express in human cervix cancer cell line HeLa, and to detect its effect on HOX family expression and cell cycle.METHODS: pEGFP-BMI-1 was transfected into HeLa cells with Lipofectamine 2000. The expression of pEGFP-BMI-1 was determined by EGFP fluorescence and Western blotting. SYBR green I real-time RT-PCR was used to quantitate mRNA expression of P16INK4a, hTERT, HOXA9, HOXB4 and HOXC13. FACS analysis was used to detect the change of cell cycle.RESULTS: In HeLa cells transfected with pEGFP-BMI-1, the results of real-time RT-PCR showed that the mRNA expressions of P16INK4a, HOXA9 and HOXC13 were reduced to 9.2%, 10.9% and 69.7%, respectively, as compared to control HeLa cells (P<0.01). However, hTERT and HOXB4 mRNA expressions did not change significantly (P>0.05). FACS analysis showed a decrease from 65.68 % to 50.53% in G1 population and a significant increase from 27.17% to 39.59 % in S population after transfection (P<0.01).CONCLUSION: BMI-1 over-expression in HeLa cells down-regulates mRNA expressions of P16INK4a, HOXA9 and HOXC13, decreases G1 population and increases S population. Therefore, BMI-1 may be involved in carcinogenesis and cancer development.  相似文献   

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