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1.
AIM: To observe the effects of folic acid (FA) on antioxidant enzyme, nitric oxide synthase (NOS) and nitric oxide (NO) in ovariectomized (OVX) rats.METHODS: Forty three-month-old female SD rats were randomly divided into 5 groups: sham group, OVX group, diethylstilbestrol group (0.03 mg·kg-1·d-1), low-dose FA group (5 mg·kg-1·d-1) and high-dose FA group (20 mg·kg-1·d-1). Gastric gavage started 1 week after operation and lasted for 10 weeks. The rats in sham group and OVX group were given distilled water instead of FA as controls. At the end of the 10th week, the L5 vertebra and right femur were removed for determination of bone mineral density (BMD). The bone homogenates were made using the L3 and L4 vertebrae. The levels of the total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), NOS and NO were detected in plasma and bone homogenates.RESULTS: Compared with sham group, the BMD levels in L5 vertebra and right femur and the levels of GSH-Px and NO in the plasma were all decreased. The levels of TAC, GSH-Px, NOS and NO in the bone homogenates were also decreased, while the MDA concentration was increased in OVX group (all P < 0.01). Compared with OVX group, the levels of TAC, GSH-Px, NOS, NO and BMD of the L5 vertebra and right femur were all increased, while the MDA concentration was decreased in high-dose FA group (all P < 0.01). CONCLUSION: In female SD rats, ovariectomy leads to a significant reduction of antioxidant enzyme, NOS and NO levels. Oxidative stress is possibly involved in the development of osteoporosis. Protection against osteoporosis by high-dose FA may be linked to improvement of antioxidant enzyme activity, the levels of NOS and NO as well as a reduction of oxidative stress in ovariectomized rats.  相似文献   

2.
AIM:To assess the antioxidative activity of a new cyclic diaryheptanoid (compound 1) from ginger on free radical damage. METHODS: Hemolysis, MDA levels of mice liver tissue homogenates, the conformation changes of irradiated PUC18 were used as indices. RESULTS:Compound 1 significantly reduced the hemolysis of human red blood cells induced by H2O2 (P＜0.01) and MDA level in the liver of mice (P＜0.01), raised the percentage of the supercoil conformation of plasmid PUC18 induced by[60Co]γ-rays (P＜0.05 or P＜0.01).CONCLUSION:Compound 1 had a good activity on suppressing oxygen free radical damage.  相似文献   

3.
AIM:To assess the antiradiation effect of oligomeric procyanidins. METHODS:The hemolysis, malondialdehyde(MDA) levels of mouse liver homogenates and the broken degree of DNA single-strain after being irradiated by ultraviolet were examined. RESULTS:Oligomeric procyanidins significantly reduced the hemolysis of erythrocytes induced by ultraviolet irradiation, inhibited the increase in MDA levels of irradiated mouse liver homogenates (P＜0.01), and raised the percentage of supercoil configuration of plasmid PUC18 irradiated with ultraviolet(P＜0.01). CONCLUSION:Oligomeric procyanidins has an antiradiation effect, the mechanism may be related to its antioxidant action.  相似文献   

4.
AIM: To observe the changes of metallothionein (MT) in various tissues of mice during hyperhomocysteinemia. METHODS: Intraperitoneal injection of homocysteine into mice induced hyperhomocysteinemia. The contents of tissue MT and malondialdehyde (MDA) in liver, heart and kidney were determined. RESULTS: Compared with control group, tissue MT levels in Hcy-group animals were increased by 210% (P＜0.01) for liver, by 133% (P＜0.05) for heart and by 60% (P＜0.01) for kidney. Tissue MDA contents were increased by 6%, 52%(P＜0.05) and 69% (P＜0.05), respectively. However, in treated animals with Hcy plus superoxide dismutase (SOD), the tissue MT contents in liver, heart and kidney were decreased by 22% (P＜0.05), 33% (P＜0.05) and 13% (P＞0.05), respectively, compared with Hcy alone group. Tissue MDA contents were decreased by 24% (P＜0.05), 21% (P＜0.05) and 48% (P＜0.01). There was a positive correlation between tissue MT content and MDA level. CONCLUSION: Hcy could induce tissue MT formation through oxidative stress.  相似文献   

5.
AIM: To observe the level of metallothionein (MT) in liver, aorta and plasma of rabbit with atherosclerosis (AS) in order to recognize the alteration of oxidative defense system in body when AS occurred.METHODS:Preparation of AS model of rabbit induced by having high-fat diet for eight weeks; the levels of MT and malondialdehyde (MDA) were measured in the tissues of liver and aorta and plasma of rabbit.RESULTS:The MT levels in liver tissues and plasma in atherosclerotic group increased 318%(P＜0.01) and 62% (P＜0.01), compared with control group, but its level in aortic tissue in atherosclerotic group decreased 33% (P＜0.01). The MDA levels in liver, aortic and plasma in atherosclerotic group increased 95%(P＜0.01), 76%(P＜0.01) and 42%(P＜0.01), respectively, compared with control group. The changes of contents of MT in liver and plasma have relation with level of MDA in liver tissues and plasma.CONCLUSION:The alteration of MT in liver tissues and plasma in atherosclerotic rabbits may be related to lipid hyperoxidative injury.  相似文献   

6.
AIM: To compare effects of SiNi-decoction and Vitamin E on vascular endothelial function of experimental atherosclerosis rabbits and their therapeutic action on atherosclerosis.METHODS:The model of experimental atherosclerosis rabbits fed with forage of high lipid was established and treated in groups randomly. At the end of the experiment, samples of aorta and blood were taken and the percentage of lipid plaque area of aortic endothelium ,lipid metabolism and vascular endothelial oxidative injury (SOD activity, MDA content, NO level, endothelin concentration) of each group were analyzed. RESULTS: In comparison with model group,the percentage of the lipid plaque area of aortic endothelium and endothelial oxidative injury (except for SOD of VitE group) of SiNi-high and mid-dose group and VitE group are reduced obviously (P＜0.05),and the index of lipid metabolism of SiNi-decoction group is improved (P＜0.05). CONCLUSION:The comprehensive therapentic effects of SiNi decoction on vascular endothelial oxidative injury and atherosclerosis are superior to VitE.  相似文献   

7.
LIU Jia-xian  CHEN Jin-he  WU Yong 《园艺学报》2005,21(12):2374-2377
AIM: To investigate the changes of the gut mucosa antioxidant system and liver, renal functions during rat intestinal ischemia-reperfusion injury. METHODS: 30 male Wistar rats underwent 45 min of intestinal ischemia by clamping the superior mesenteric artery followed by reperfusion. The levels of malondialdehyde (MDA), glutathione (GSH), the activities of antioxidant enzymes in the gut mucosa including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), glutathione S- transferase (GST) activity and serum ALT, AST, BUN, Cr were assayed at 2, 4, 8, 12 and 24 h after reperfusion. RESULTS: The levels of MDA and GSH in the gut mucosa increased and decreased significantly at 2 h of reperfusion, respectively (P<0.05). MDA was still lower than sham at 24 h of reperfusion (P<0.05), while GSH decreased to 40% of sham at 4 h of reperfusion (P<0.01) but returned to the level of control at 12 h. The activities of CAT, SOD and GSH-Px did not show significant changes in rat after intestinal ischemia reperfusion. GST decreased 39% at 2 h of reperfusion compared with the sham group and decreased to 56% of sham at 4 h (P<0.05), but returned to the level of control at 12 h after reperfusion. Serum ALT, AST, BUN and Cr increased significantly at 2 h of reperfusion (P<0.05) and increased 208%, 100%, 103%, 41% compared with control at 4 h of reperfusion (P<0.01). However, at 24 h of reperfusion, they returned to normal. CONCLUSION: Intestinal ischemia/reperfusion diminishes GSH level and GST activity, increases MDA level and causes liver and renal reversible damages.  相似文献   

8.
AIM:Scopolamine blocks cholinergic transmission and impairs learning and memory in mice. The purpose of this study was to evaluate the memory-improving properties of curcumin on scopolamine-induced memory impairment in mice. METHODS:The mice of memory impairment were induced by scopolamine. Step down test and Morris water maze test were used to observe the learning and memory ability in curcumin-treated mice. Biochemical assessments of AChE, MDA, and GSH-Px levels in brains were performed. RESULTS:Oral administration of curcumin significantly reduced the numbers of step-down errors (P<0.05) and prolonged the step-down latency induced by scopolamine (P<0.05). The escape latency time in mice treated with curcumin was remarkably reduced compared to that in scopolamine group by Morris water maze test (P<0.05). After the platform was removed, the total time that the mice swam in the target quadrant was also longer in curcumin group than that in model group (P<0.05). The data also indicated that curcumin significantly inhibited AChE activity (P<0.01) and prevented oxidative stress characterized by the significant reduction in MDA content and the significant increase in GSH-Px activities in the brain (P<0.01). CONCLUSION:Curcumin induces cognitive improvement by enhancing the function of cholinergic system and its antioxidant activity.  相似文献   

9.
AIM: To investigate DNA damage and cell stress (heat shock protein 70 expression) in human bronchi smooth muscle cells by cigarette smoke extract (CSE) in vitro. METHODS: 30 mL smog was dissolved in 1 mL culture medium as stock solution of CSE. Human bronchi smooth muscle cells were cultured 3 hours with 1∶16, 1∶10, 1∶8, 1∶6 and 1∶4 of CSE. The DNA damage and HSP70 expression were determined by single cell gel assay (comet assay) and Western blot, respectively. RESULTS: Associated with rising CSE concentration, DNA damage aggravated. Compared with the untreated group, except 1∶16 of CSE, the level of DNA damage was significantly different (P＜0.05). The level of HSP70 expression rised in 1∶16 and 1∶10 of CSE, but it gradually decreased in others. There was significantly different in the fifth and sixth group with negative control (P＜0.05). CONCLUSION: CSE results in DNA damage and decreasing of HSP70 expression, which may be related with chronic obstructive pulmonary diseases.  相似文献   

10.
AIM: To observe the role of exogenous and endogenous basic fibroblst growth factor (bFGF) on myocardial ischemia/reperfusion(I/R) injury of rats.METHODS:bFGF and bFGF antiserum were applied to rat isolated I/R heart. Myocardial function, coronary effluent volume,protein and myoglobin content as well as LDH activity in coronary effluent fluid, myocardial calcium, MDA and ATP concentration as well as PKC, MAPK activity were measured. RESULTS:Compared with control, myocardial function in I/R group significantly decreased. Protein, myoglobin content and LDH activity in coronary effluent liquid as well as myocardial MDA and calcium content increased, while myocardial ATP concentration decreased(all P＜0.01). Compared with I/R group, ±LV dp/dtmax in bFGF group increased by 43% and 26%, respectively. LVEDP decreased by 40%. HRr/HRi and B/A augmented by 42% and 20%, respectively. Protein and myoglobin content as well as LDH activity lowered by 29%,30％ (all P＜0.01) and 33％ (P＜0.05) respectively. Myocardial MDA and calcium content decreased by 44% and 35%, respectively, while myocardial ATP level as well as PKC and MAPK activity increased by 34%,41％ and 10% (all P＜0.01), respectively. In bFGF antiserum group, ±LV dp/dtmax were 35% and 38% lower than those in I/R group. LVEDP increased by 93%. HRr/HRi and B/A decreased by 36% and 45%, respectively. Protein and myoglobin content as well as LDH activity augmented by 54%,96％ (all P＜0.01) and 34％ (P＜0.05) respectively. Myocardial MDA and calcium content increased by 24% and 50%, respectively, while myocardial ATP level as well as PKC and MAPK activity lowered by 28%,21％ and 8% (all P＜0.01), respectively. CONCLUSION:Endogenous bFGF is a protective factor against myocardial ischemia/reperfusion injury of rats.  相似文献   

11.
AIM: To study the relationship between multidrug-resistance (MDR1) gene product P-glycoprotein (P-gp) and the volume-activated chloride current. METHODS:The volume-activated chloride current in bovine non-pigmented ciliary epithelial cells was recorded using a whole cell recording technique. An antisense technique was used to inhibit the expression of MDR1 gene. The immunofluorescence of P-gp was monitored with a real-time laser confocal microscope.RESULTS:P-gp immunofluorescence correlated negatively with the concentration of the human MDR1 antisense oligonucleotide. The antisense oligonucleotide inhibited the volume-activated chloride current specifically and partially. The latency of activation of the current increased and the peak current decreased. The percentage of inhibition of peak current correlated positively to the concentration of the antisense oligonucleotide(r＝0.99, P＜0.01) and to the reduction(%) of P-gp immunofluorescence(r=0.99,P＜0.01).CONCLUSION:P-gp, the product of MDR1 gene, plays an important role in the activation pathway of volume-activated chloride current in non-pigmented ciliary epithelial cells.  相似文献   

12.
AIM: To investigate the changes of oxidative stress in the stomach tissues and their roles in gastric motility and interstitial cells of Cajal (ICC) in diabetic rats. METHODS: Thirty-eight SD rats (8-week-old, male) were intraperitoneally injected with streptozotocin (STZ). Diabetes was successfully induced in 36 of them. The diabetes rats were randomly divided into untreated diabetes group and treated diabetes group. Eighteen healthy SD rats (8-week-old, male) served as controls. The body weight and the levels of blood glucose and glycosylated hemoglobin were measured. At the end of week 1 and week 10, 9 rats were sacrificed in each group. The gastric emptying rate and the levels of malondialdehyde (MDA),superoxide dismutase (SOD), tumor necrosis factor α (TNF-α), tyrosine kinase receptor c-Kit and stem cell factor (SCF) in gastric smooth muscle were analyzed. The apoptosis of ICC in gastric tissues was detected by the methods of immunocytochemistry and TUNEL. RESULTS: Compared with control group, gastric motility and SOD activity in untreated diabetes group were significantly weakened, the levels of MDA and TNF-α increased, the levels of c-Kit and SCF decreased, and apoptosis of ICC enhanced. In treated diabetes group, the oxidative stress level was attenuated, antioxidant capacity was enhanced, the levels of c-Kit and SCF were significantly increased, and the ICC apoptosis was reduced. Gastric motility was significantly improved after antioxidant therapy. CONCLUSION: Hyperglycemia affects the expression of antioxidant enzymes in the stomachs of diabetic rats. Oxidative stress is caused by hyperglycemia and is an important factor in the etiology of gastric motility dysfunction in diabetic rats, which may be correlated with the augmentation of ICC apoptosis resulting from oxidative stress-induced c-Kit/SCF damage.  相似文献   

13.
AIM: To investigate the effects of N-acetylcysteine (NAC) combined with azithromycin (AZI) on oxidative stress in the rats with chronic obstructive pulmonary disease (COPD). METHODS: Male Wistar rats (n=60) were randomly divided into control group, model group, AZI intervention group,NAC intervention group and AZI+NAC group. The COPD model was established by passive smoking and intratracheal instillation of lipopolysaccharide. Each day 30 min prior to smoking, intragastric administration with AZI, NAC or combination of the 2 drugs was given for AZI, NAC, and AZI+NAC groups, respectively. On the 31st day, all rats were killed following lung function test. Cell counts of bronchoalveolar lavage fluid (BALF) were performed, and the contents of interleukin-8 (IL-8), interleukin-17 (IL-17) and tumor necrosis factor alpha (TNF-α) in BALF were measured by ELISA. The histopathology of the lung tissues was observed under light microscope, and the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the lung homogenate were measured. RESULTS: Compared with control group, the other 4 groups showed decreased pulmonary function, and inflammatory cell infiltration and alveolar destruction in histopathology. Compared with control group, the other groups showed higher white blood cells, monocyte-macrophages, neutrophils and lymphocytes in the BALF (P<0.05). Compared with model group, AZI group and NAC group, lower white blood cells, neutrophils and lymphocytes in the BALF were observed in AZI+NAC group (P<0.05). Compared with model group, IL-8, IL-17, TNF-α and MDA in AZI group, NAC group and AZI+NAC group significantly decreased (P<0.05), while SOD and GSH-Px significantly increased (P<0.05). Compared with AZI or NAC group, IL-8, IL-17, TNF-α and MDA in AZI+NAC group significantly decreased (P<0.05), while SOD and GSH-Px increased significantly (P<0.05). CONCLUSION: Both NAC and AZI attenuate the lung inflammation and oxidative damage in COPD model rats. Combined medication exerts preferable anti-oxidation effects, which might be more suitable for the treatment of COPD.  相似文献   

14.
AIM: To study the effect of β-amyloid protein (β-AP) and D-galactose(D-gal) on learning-memory and SOD activity and MDA content of hippocampus in rats. METHODS: The behaviors of rats were measured by using open field, Y-maze and one-trial passive avoidance response, and the content of SOD and MDA were measured. RESULTS: In the D-gal and D-gal+β-AP group rats, the spontaneous activities and responses to novel environment in the open field were significantly decreased, and the abilities of learning-memory were remarkably attenuated, the content of SOD decreased and MDA content increased markedly in hippocampus (P＜0.05 or P＜0.01 compared with N group). CONCLUSION: The combination of β-AP and D-gal enhanced the damage induced by free radical in hippocampus and decreased the learning-memory ability in rats.  相似文献   

15.
AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A2(PLA2) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA2 activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.  相似文献   

16.
AIM: To investigate the effect of bilirubin on acute lung injury (ALI) and the mechanism. METHODS: 30 male Wistar rats were divided into normal group, ALI group and bilirubin treatment group. Lung specimens were examined by histopathological technique. Lung index (LI) and lung permeability index (LPI) were measured. Moreover, white blood cell (WBC) count, neutrophil percentage (PMN%) and the content of protein (Pr) in the bronchoalveolar lavage fluid (BALF), as well as the contents of superoxide dismutase (SOD), malonaldehyde (MDA) and glutathione peroxidase (GSH-Px) in the lung homogenate were determined. RESULTS: (1) In ALI group: LI, WBC count, PMN%, Pr and LPI increased significantly compared with normal group (P<0.01). In bilirubin treatment group, all the values determined decreased compared with ALI group (P<0.01; P<0.05). No notable discrepancy between bilirubin treatment group and normal group (P>0.05) was observed. (2) In ALI group, the content of MDA was significantly higher (P<0.01), but the contents of SOD and GSH-Px were significantly lower than those in normal group (P<0.01). In bilirubin treatment group, the content of MDA decreased significantly (P<0.01) but the contents of SOD and GSH-Px increased significantly (P<0.01; P<0.05) compared with ALI group. No notable discrepancy between bilirubin treatment group and normal group was observed (P>0.05). CONCLUSION: Bilirubin relieves ALI induced by LPS in rats via antioxidation.  相似文献   

17.
AIM:To investigate the regulatory effects of homocysteine (Hcy) on the viability and migration of rat basilar arterial smooth muscle cells (BASMCs) and its potential molecular mechanisms. METHODS:BASMCs were isolated, cultured in vitro and treated with Hcy at different concentrations. The cell viability was measured by CCK-8 assay, and the activation of Rho kinase pathway was measured by Western blot. The cells were treated with Hcy at fixed concentration (1 mmol/L), and ROCK inhibitor Y-27632 was also used. The cell cycle distribution was analyzed by flow cytometry. The cell migration ability was detected by wound healing assay and Transwell assay. The activation of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the level of malondialdehyde (MDA) were measured for determining the status of oxidative stress.RESULTS:Hcy increased the viability of BASMCs and the protein expression of GTP-RhoA and ROCK2 in a dose-dependent manner (P<0.05). Compared with the cells treated with Hcy for 24 h, the cells treated with Hcy for 48 h had enhanced viability (P<0.05). Compared with control group, treatment with Hcy increased cell population in S phase and decreased cell population in G0/G1 phase, while pre-incubation with Y-27632 reversed Hcy-induced G1/S phase transition in BASMCs (P<0.05). The cell migration rate in Hcy treatment group was remarkably higher than that in control group(P<0.05), while pre-incubation with Y-27632 reversed Hcy-induced cell migration (P<0.05). Furthermore, Hcy inhibited the activation of SOD and GSH-Px, accompanied with increased MDA level (P<0.05). Compared with Hcy treatment group, pre-incubation with Y-27632 increased the activation of SOD and GSH-Px, but decreased MDA level (P<0.05). CONCLUSION:Homocysteine induces the viability and migration of rat BASMCs, and its mechanisms may be related to activation of Rho kinase pathway.  相似文献   

18.
AIM: To assess the degree of oxidative damage during acute myocardial infarction and reperfusion, and to clarify the protective effect of Tongxinluo in mini-swine model. METHODS: Thirty mini-swines were randomized into 5 study groups: sham group, model group, low dose (0.05 g·kg-1·d-1), medium dose (0.2 g·kg-1·d-1) and high dose (0.5 g·kg-1·d-1) of Tongxinluo groups (pretreated with Tongxinluo for 3 d). Animals except in sham group were subjected to 3 h of coronary occlusion followed by 1 h of reperfusion. Concentrations of total antioxidative capability (T-AOC), total superoxide dismutase (T-SOD), reduced glutathione (GSH) and malondialdehyde (MDA) in blood sample and the myocardium were measured. RESULTS: (1) T-AOC, T-SOD and GSH in serum significantly decreased (all P<0.05), while MDA significantly increased (P<0.01) at 3 h after AMI in comparison with those at baseline. Compared to those at 3 h after AMI, the contents of T-AOC, T-SOD and GSH at 1 h after reperfusion significantly decreased (all P<0.01), accompanied by increase of MDA (P<0.01). (2) Compared to those in normal area, levels of T-AOC, T-SOD and GSH in reperfusion myocardium decreased significantly (all P<0.01) and MDA increased significantly (P<0.01). T-AOC, T-SOD and GSH in no-reflow myocardium further decreased (all P<0.01) and MDA increased (P<0.01) as compared to those in reperfusion myocardium. (3) Compared to model group, medium dose of Tongxinluo increased the contents of T-AOC and T-SOD and reduced MDA production in serum at 3 h after AMI (all P<0.05), while medium dose of Tongxinluo increased T-SOD level at 1 h after reperfusion (P<0.05). High dose of Tongxinluo increased the levels of T-AOC and T-SOD and decreased MDA content in serum at 3 h after AMI and 1 h after reperfusion (all P<0.05). (4) The medium dose of Tongxinluo increased T-AOC content (P<0.05) and reduced MDA (P<0.05) in reperfusion myocardium, while high dose of Tongxinluo increased T-AOC, T-SOD and GSH (all P<0.05), reduced MDA (P<0.01) in reperfusion myocardium, and also increased T-AOC, T-SOD (all P<0.05), reduced MDA (P<0.01) in no-reflow area as compared to those in model group. CONCLUSION: Impairment of antioxidant defense system in vivo and imbalance of redox homeostasis in myocardium region might play an important role in the pathogenesis of no-reflow after myocardial acute infarction following reperfusion. Tongxinluo protects myocardium from reperfusion injury by improving antioxidant defense and attenuating oxidative damage.  相似文献   

19.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.  相似文献   

20.
AIM:To investigate the correlation between the signal pathway of IKK/NF-κB and the anti-oxidant activity in asthmatic rats and the modulation of Ginkgo biloba extract (Egb). METHODS:Thirty-six male SD rats were randomly divided into three groups:control group (group C), asthmatic group (group A) and Egb group(group E). Asthma in rats was established by ovalbumin (OVA) challenge methods. The mRNA of IKKβ and the protein of NF-κB P65 in lung tissue were assessed by using in situ hybridization with oligonucleotide probe and immunohistochemisty, respectively. RESULTS:The expression of IKKβ mRNA and NF-κB P65 protein in group A were significantly increased when compared with group C (P＜0.01, respectively), but those data in group E were significantly decreased when compared with group A (P＜0.01, respectively). The concentrations of MDA in serum and BLAF in group A were significantly higher than those in control group (P＜0.01，respectively), but the concentrations of MDA in group E were significantly lower than those in group A (P＜0.01, respectively). The concentrations of GSH in group A were significantly lower than those in group C (P＜0.01, respectively), but the concentrations of GSH in group E were significantly higher than those in group A (P＜0.01, respectively). CONCLUSION:The expression of IKKβ mRNA and NF-κB P65 protein were increased significantly in asthmatic rats. This was possibly attributed to the decreased anti-oxidant activity. Egb increased the anti-oxidant activity and inhibited the activity of IKK/NF-κB.  相似文献   

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