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1.
基因芯片检测转基因油菜   总被引：11，自引：0，他引：11  
在设计转基因油菜(Brassica napus)的基因芯片检测方法时，根据油菜中所转入的外源基因，选择了CaMV35S启动子、FMV35S启动子、Nos终止子、Bar基因、Barnase基因、Barstar基因、EPSPS基因、GOX基因、PAT基因和内源基因Fbp等设计了引物对与探针，并制备了寡核甘酸芯片，通过多重PCR对样品核酸进行扩增和荧光标记后，将PCR产物与芯片杂交，检测油菜样品中所含的外源基因。结果表明，实验有较好的特异性和重复性，在检测低含量的转基因油菜时灵敏度可达到0．5％。由于采用了多重PCR和芯片的多基因并行杂交的技术，一次可同时检测10个基因，在检测多品种混合的转基因油菜商品时具有独特优势。  相似文献   

2.
抗亚洲玉米螟、抗草甘膦转基因玉米的培育   总被引：2，自引：0，他引：2  
5-烯醇式丙酮酸莽草酸-3-磷酸合酶基因(5-enolpyruvyl-shikimate-3-phosphate synthase,epsps)是一个高抗草甘膦的基因,苏云金芽胞杆菌(Bacillus thuringiensis)的杀虫蛋白基因(Bt基因)cry1Ac M是一个能在单子叶植物中高效表达的抗亚洲玉米螟基因。为了获得具有抗虫、抗除草剂优良复合性状的转基因玉米,本研究构建出质粒p CAMBIA1302-P35S::epsps-Tnos-Pubi::cry1Ac M-Tnos,通过农杆菌(Agrobacterium tumefacien)介导的玉米(Zea mays)茎尖遗传转化法将目标基因转入玉米。通过分子检测、草甘膦抗性筛选和田间接种亚洲玉米螟(Ostrinia furnacalis(Guenée))试验筛选出转基因株系,在此基础上进行了转基因植株室内抗亚洲玉米螟试验和田间草甘膦抗性分析,并采用RT-PCR检测和Western blot方法确定了抗虫蛋白在转基因植株中稳定表达。从大量转基因株系中优选出6个遗传稳定且抗亚洲玉米螟、抗除草剂草甘膦的转基因玉米株系,这些株系的抗亚洲玉米螟、抗草甘膦特性完全满足大田应用的需求。综上所述,将改造后的Bt抗虫基因cry1Ac M和抗草甘膦基因epsps一同转入玉米,赋予两种玉米骨干自交系植株抗玉米螟、抗草甘膦特性,为我国抗亚洲玉米螟抗草甘膦转基因玉米的大面积推广培育出了优良自交系。  相似文献   

3.
为确定猪生长激素基因(pGH)在F1代转基因猪体内的遗传稳定性及其在育肥初期表达水平的差异,利用SYBRGreenⅠReal-time PCR法检测精子介导纳米基因载体法获得的F0代母猪与非转基因公猪配种所产的7头转pGH基因猪和9头非转基因猪3月龄生长激素的表达水平。根据16个血液样品荧光定量结果绘制溶解曲线和扩增曲线图谱,并用F=(1+E)﹣ΔΔCt,公式进行Ct均值的分析,得出F1代转pGH基因猪生长激素平均表达水平是同期非转基因猪的1.77倍。结果证实,1)外源pGH基因在转基因猪体内能稳定传代;2)并非每头转基因猪体内pGH基因的表达量均高于非转基因猪;3)在F1代转基因猪体内,pGH的平均表达水平显著高于同期非转基因猪。本实验建立了能够稳定测评家猪生长激素相对基因表达水平的方法,为研究转基因与非转基因动物之间生理指标与定量表达的差异性等提供了科学依据。  相似文献   

4.
快捷、准确地检测转基因植物中外源基因的拷贝数,是转基因生物育种的重要研究内容,具有较大的应用前景。本研究以7个抗虫BT(Bacillus thuringiensis)和抗草甘膦EPSPS-G10转基因大豆事件为材料,以SYBR Green I为荧光染料,利用实时荧光定量PCR方法,根据PCR反应获得的Ct值与起始模板数的对数值存在线性反比关系这一原理,建立了相关性系数在0.99以上的模板定量标线。通过目的基因与内参基因——大豆肌动蛋白编码基因ACTIN的起始模板量比较,估算出各株系的目的基因拷贝数。数据显示,株系1、2、3和4的2个基因均为单拷贝,株系6的2个基因均有2个拷贝,株系5和7的2个基因的拷贝数为3。利用Southern印迹方法对材料1~4的拷贝数进行验证,结果表明,两者的检测结果基本一致,证明实时荧光PCR检测法是大豆外源基因拷贝数检测中快速有效的方法。实时荧光PCR高效快速检测基因拷贝数,对于大规模转基因株系的外源基因拷贝数检测应用具有重大意义。  相似文献   

5.
转基因产品中抗除草剂基因的PCR检测   总被引：1，自引：0，他引：1  
抗除草剂作物占全球转基因作物的75％以上，并且这类作物还有不断增加的趋势。首次建立并优化了对4类转基因作物（大豆（Glycine max）、玉米（Zea mays）、棉花（Gossypium hirsutnm）和油菜（Brassica campestris var.oleifera））的5种外源抗除草剂基因（bar、pat、cp4-epspsI、cp4-epsps2和gox）的PCR检测方法，并在实践中应用。结果表明，本方法灵敏特异、结果准确可靠，可对目前批准的所有抗除草剂转基因产品进行定性检测。  相似文献   

6.
5-烯醇式丙酮酰莽草酸-3-磷酸合酶(EPSPS)催化莽草酸-3-磷酸(S3P)与磷酸烯醇式丙酮酸(PEP)合成5-烯醇式丙酮酰莽草酸-3-磷酸(EPSP),具有与草甘膦结合的活性位点,且结合后会抑制EPSPS的活性,在植物抗除草剂基因工程中具有重要的应用价值.为了培育抗草甘膦玉米(Zea mays),本研究通过对高粱(Sorghum bicolor)EPSPS基因的结构分析,克隆了该基因5'端的叶绿体转运肽(chloroplast transit peptide,CTP).将该转运肽与来源于农杆菌(Agrobacterium tumefaciens)菌株CP4 EPSPS基因整合,以Ubiquitin为启动子,35S polyA为终止子,构建表达载体,同时以不含有转运肽的CP4 EPSPS基因为对照,遗传转化玉米得到稳定转基因株系;用PCR、Southern blot、ELISA等方法检测转基因玉米CP4 EPSPS基因的表达量并对其进行草甘膦抗性检测,研究发现,不含转运肽的转化事件虽然CP4 EPSPS基因表达量与含有转运肽的基本一致但却不具有草甘膦抗性,而含有转运肽的转化事件则抗性明显.说明转运肽并不影响CP4 EPSPS基因的表达,但对转基因植株草甘膦抗性起着重要作用,说明本研究克隆的叶绿体转运肽能够正常行使其生物学功能.研究结果为利用EPSPS基因培育抗草甘膦作物提供了重要参考资料.  相似文献   

7.
以从抗草甘膦的荧光假单胞菌(Pseudomonas fluorescens)G2中克隆的、并按双子叶植物偏爱密码子改造的5-烯醇式丙酮酰莽草酸-3-磷酸合酶(EPSPS)基因aroAG2M为目的基因,用菊花Rubisco小亚基的启动子驱动,在基因的5'端加叶绿体定位信号肽,构建植物表达载体。用根癌农杆菌(Agrobacterium tumefaciens)介导的叶盘法转化烟草(Nicotiana tabacum)获得转基因植株,PCR检测表明,外源基因已整合到烟草基因组中。转基因和非转基因植株6～8叶龄苗的叶片涂抹不同浓度的草甘膦异丙胺盐,表明转基因植株可抗4‰浓度的草甘膦,而非转基因对照植株则在2‰草甘膦时即死亡。花粉管通道法转化棉花(Gossypium hirsutum),得到3株具有草甘膦抗性的转基因植株,PCR和Southern检测显示,外源基因已整合到棉花基因组中,田间喷洒草甘膦异丙胺盐水剂,表明T1代转基因植株具有草甘膦抗性。  相似文献   

8.
内标准基因(endogenous reference gene)是指具有物种专一性、不显示等位基因变化、拷贝数恒定的保守DNA序列,对基因定量分析时具有重要意义.本研究选取了5个水稻内标准基因:根部表达的水稻基因(rice root-specific,GOS9)、磷脂酶D基因(phospholipase D,PLD)、蔗糖磷酸合成酶基因(sucrose phosphate synthase,SPS)、水稻淀粉分支酶基因(rice starch branching enzyme,RBE4)、泛素蛋白基因(ubiquitin 5,UBQ5)和2个外源基因:苏云金芽孢杆菌CrylAb杀虫晶体蛋白基因CrylAb和cryl Ab/cryl Ac融合杀虫晶体蛋白基因(CrylA c/CrylAb),分别以转基因水稻(Oryza sativa L.)克螟稻2号和TT51-1为模板,比较各自的外源基因(CrylAb =KMD2和CrylA c/CrylA b=TT51-1)与5个内源基因的PCR产物量,筛选出和外源基因PCR产物量最接近的内标准基因为RBE4.在此基础上,数字PCR不依赖于已知浓度的标准曲线来定值,可以避免标准样品的标准曲线和样品目的基因的扩增曲线在扩增效率上的不一致等因素所带来的误差,定值结果更加准确、可靠,采用数字PCR分析外源基因拷贝数与内标准基因RBE4拷贝数的比值,荧光定量PCR方法分析内标准基因RBE4和外源基因的定量检测稳定性、灵敏度等.结果表明,外源基因拷贝数与内标准基因RBE4拷贝数的比值在转基因水稻克螟稻2号和TT51-1上都较接近于1∶1,分别为115.9％和105.3％.RBE4的荧光定量PCR体系重复性、定量检测稳定性和灵敏度好,符合作为转基因水稻基体标准物质定量分析的内标准基因的要求,RBE4定量体系在TT51-1和克螟稻2号的最小检出限(LOD)分别为5～11 copies/μL和3～12 copies/μL,最小定量限(LOQ)分别为11～22和12～24 copies/μL.RBE4是转基因水稻标准物质研制和产品定量检测的适宜内标准基因.研究结果为转基因作物标准物质研制和定量检测的内标准基因选取提供一定的参考.  相似文献   

9.
中国转基因油菜的环境安全性分析   总被引：15，自引：0，他引：15  
根据中国转基因油菜研究和环境释放的现状，对中国环境条件下转基因油菜的生存竞争能力、基因扩散风险以及对生物多样性影响等环境安全问题进行了分析。分析认为，中国是许多十字花科植物进化起源中心和生物多样性中心，生态环境类型复杂；转基因油菜的外源基因可以通过花粉向油菜（Brassica napus）、白菜（B．rapa）和芥菜（B．juncea）类植物扩散；中国转基因油菜的基因扩散风险高于其它作物．存中国，油菜的转基因技术相当成熟，油菜的转基因实验频次很高，涉及的基因种类繁多，进口的转基因油菜数量巨大，隐患不容忽视。在中国，转基因油菜的安全性管理和研究相当薄弱，检测和监测手段无法满足安全管理需求，这一现状必将阻碍中国转基困油菜的产业化进程，并对中国环境安全带来隐患。作者提出，加强转基因油菜的环境安全意识，加强中国转基冈油菜的安全管理及其措施的落实，加强转基因油菜检测和监测等技术平台的建设。  相似文献   

10.
在利用实时荧光定量PCR(Real-time quantitative PCR,q RT-PCR)检测转基因成分的含量时,数据分析的规范化与标准化对保证实验数据的准确性及实验室间数据的可比性具有重要意义。本研究以转基因玉米(Zea mays)NK603为研究材料,分析了分别以q RT-PCR中3次平行反应的单个Ct值和3次平行反应Ct值的平均值所绘制标准曲线的差异;分析了分别以盲样3次平行反应的Ct平均值和3次平行反应的拷贝数平均值(算术平均值或几何平均值)计算转基因成分含量的差异;并研究了结果准确性判定的方法。结果表明,标准曲线应基于单个Ct值;在计算转基因成分的含量时,Ct值应取算术平均值,拷贝数应取几何平均值;最后通过比较样品的测量结果与标准值之间的差异是否显著(UΔ>Δm,表明测量结果的平均值与样品的标准值无显著差别)来判断实验结果的可靠性。本研究为转基因产品检测中q RT-PCR实验数据处理的标准化提供了参考资料。  相似文献   

11.
The results from this research indicate that canola meal (CM) can be used for adsorption of Zn2+, Cd2+, Cu2+, Pb2+and Ni2+from aqueous solutions. The order of sorption for these metals in single metal systems was as follows (molar basis): Zn2+> Cu2+> Cd2+> Ni2+> Pb2+. It was noted that a decrease in the concentration of CM caused a higher metal loading on the meal. Increases in the metal concentration, temperature or pH resulted in increased sorption of the metals by the meal. The systems with identical ratios of CM to Zn2+concentrations, regardless of their levels, resulted in the same amount of metal adsorbed per unit weight of meal. The Freundlich isotherm type model was used in this study and was found to fit the experimental equilibrium concentration data of Zn2+and Cd2+; however, the Langmuir isotherm model fit only the equilibrium data of Zn2+. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray (EDX) microanalyses revealed that the metal ions were sorbed mainly at the cell wall and only small amounts of ions diffused into the cytoplasm of the CM cells. The Electron Spin Resonance (ESR) tests were inconclusive regarding the direct participation of free radicals in copper sorption.  相似文献   

12.
Spring wheat (Triticum aestivumL.) is the major crop species grown in south-western Australia and no responses of wheat to applied calcium (Ca) have been obtained in field experiments though responses have been obtained in glasshouse pot studies for wheat grown on the predominantly sandy acidic soils of the region. Since the mid 1990s canola (oilseed rape, Brassica napus L.) has been grown in rotation with wheat and has often developed symptoms of Ca deficiency when grown on sandy acidic soils in the field. The Ca requirement of canola in these soils is not known and was measured and compared with Ca requirements of wheat in the glasshouse study reported here when 5 amounts of Ca (0–630 mg Ca/pot), as calcium sulfate, were applied.

Application of Ca did not affect shoot production of wheat but increased grain yields by about 25% and 50 mg Ca/pot was required to produce 90% of the maximum grain yield. Two canola cultivars were grown, and both showed no shoot yield responses to applied Ca at early growth (GS1.5). However, at flower bud visible (GS3.5) shoots of triazine tolerant canola cv. ‘Karoo’ showed about 17% increase to applied Ca and required ～47 mg Ca/pot to produce 90% of the maximum yield, while corresponding values for cv. ‘Outback’ were 42% and 185 mg Ca/pot. Both canola cultivars showed large seed (grain) yield responses to applied Ca. Canola cv. ‘Outback’ produced no grain when no Ca was applied and showed ～ 97% increase to applied Ca and required about 462 mg Ca/pot to produce 90% of the maximum grain yield. The triazine tolerant cv. ‘Karoo’ produced about 22% of the maximum grain yield when no Ca was applied, showed approximately 78% grain yield response to applied Ca and required about 475 mg Ca/pot to produce 90% of the maximum grain yield. However, to produce 50% of the maximum grain yield, cv. ‘Outback’ required 250 mg Ca/pot while cv. ‘Karoo’ required about 100 mg Ca/pot. The grain yield response curve for cv. ‘Karoo’ was exponential and that for cv. ‘Outback’ was sigmoid so differences in the response curves were largest when small amounts of Ca were applied and decreased as more Ca was applied. Evidently canola cultivars differ in their ability to access soil and applied Ca providing opportunity to breed and select cultivars efficient at accessing soil and applied Ca. For both wheat and canola the concentration of Ca in dried shoots increased as more Ca was applied and, for each amount of Ca applied, the concentration of Ca in shoots decreased as plants matured. Both canola cultivars consistently had larger concentrations of Ca in shoots than wheat, either when no Ca was applied and for each amount of Ca applied, suggesting canola roots were better able to access soil and applied Ca than wheat roots. The Ca concentration in young wheat (GS15) and canola (GS1.5) shoots that was related to 90% of the maximum grain yield (critical Ca concentration) was 0.33% for wheat and 2.5% for both canola cultivars.  相似文献   

13.
《Journal of plant nutrition》2013,36(7):1145-1161
Abstract

Production of new high-yielding canola hybrids has been extremely prolific and, as a consequence, very little work has been performed to assess the fertility requirements of these crops. A series of experiments (14 site-years) was carried out over three years (1999–2001), primarily to assess the nitrogen (N) fertility of canola hybrid cultivars and at the same time ascertain whether the associated phosphate and sulfur (S) fertility are influenced by N application. All experiments included 12 rates of N (0 to 220 kg N ha?1 in 20 kg ha? 1 increments) and three rates of either P2O5 or S (0, 20, and 40 kg ha? 1) with blanket application of other nutrients. Although differences in the performance of individual canola hybrid cultivars can be significant in some cases, the term “hybrid” in this study does not refer to the performance of one specific cultivar, but to the group of hybrids tested. Under an identical nutrient regime, on average, hybrid cultivars produced a 17% higher seed yield, but did not reach maximum potential; on average, this result, occurred under a higher N fertility regime at which hybrid cultivars produced 33% higher yields than did conventional cultivars. To maintain maximum yield, hybrids must be supplied with phosphate and S at levels that are similar to those used on conventional cultivars. Hence, it would appear that hybrids are more efficient scavengers of soil nutrients, a fact that may have serious ramifications for the fertility of the following crops.  相似文献   

14.
Applying lime to ameliorate soil acidity has been observed to induce manganese (Mn) deficiency in canola (Brassica napus L.) crops grown on acid sandy soils near Albany and gravelly acid sands of the Great Southern Districts of southwestern Australia. These soils were often Mn-deficient in patches for wheat (Triticum aestivum L.) production when they were newly cleared for agriculture requiring application of Mn fertilizer to ensure grain yields were not reduced by the deficiency. Since then, these soils have acidified and in the 1990s, canola started to be grown on these soils in rotation with wheat and lupins (Lupinus angustifolius L.). These limed soils may now have become marginal to deficient in Mn for canola production. The effect of liming may change the effectiveness of fertilizer Mn. In addition, the effect of liming on the residual value of Mn fertilizer applied to these soils for canola production is unknown. Therefore, a glasshouse experiment was conducted using Mn deficient sand. Three levels of finely-powdered calcium carbonate were added and incubated in moist soil for 42 days at 22±2°C to produce 3 soils with different pH values [1:5 soil:0.01 M calcium chloride (CaCl2)]: 4.9 (original soil), 6.3, and 7.5. Five Mn levels, as solutions of Mn sulfate, were then added and incubated in moist soil for 0, 50, and 100 days before sowing canola. To estimate the residual value (RV) of incubated Mn for canola production, the effectiveness of the incubated Mn was calculated relative to the effectiveness of Mn applied just before sowing canola (freshly-applied Mn). The RV of the incubated Mn was determined using yield of dried canola shoots, the Mn application level required to produce 90% of the maximum shoot yield, and Mn content in dried shoots (Mn concentration in shoots multiplied by yield of dried shoots). As measured using both yield of dried shoots and Mn content of dried shoots, the residual value of Mn decreased with increasing soil pH and with increasing period of incubation of Mn with moist soil. The critical Mn concentration, for 90% of the total yield of dried canola shoots, was (mg Mn kg?1) ～17 in youngest mature growth (apex and youngest emerged leaf, YMG), and ～22 for the rest of dried shoots. These values were similar to current critical values for un-limed soils suggesting critical Mn concentrations remain the same for limed soils. Plant testing of canola is recommended if soils are to be limed to ameliorate soil acidity. When plant tests indicate a high likelihood of Mn deficiency, foliar Mn sprays need to be applied to that crop to ensure Mn deficiency does not reduce grain production that year, and fertilizer Mn needs to be re-applied to the soil when sowing the next crop to reduce the likelihood of Mn deficiency for subsequent crops.  相似文献   

15.
Eurasian Soil Science - In order to study the root-induced changes on behavior of phosphorus in the rhizosphere of two canola cultivars in order to better management of P fertilization this...  相似文献   

16.
Production of oilseed crops requires balanced fertilization, while environmental problems of applied fertilizers must be considered. We evaluated seven winter canola cultivars (Brassica napus L.) for their relative efficiency to use or acquire phosphorus (P) under deficient and sufficient conditions. Average root mean diameter (RMD), total root length (RL) and root surface area (RA), of plants were measured as well as rhizosphere properties in rhizobox technique. Water-soluble P (WSP) and phosphatase activity of treatments containing plant were higher than control. WSP increased by 2.86 mg kg?1 soil in Gabriela and 2.63 mg kg?1 soil in Elvis at P deficient condition, compared to the control soil. The Olsen extractable P of the treatments decreased compared to control. Variations in total dry weights of cultivars were mainly explained by the differences in P solubilizing bacteria (PSB) population, pH, and phosphatase activity. The responsible mechanism for the P efficient (PE) cultivars can be higher P uptake through larger root and changes in rhizosphere properties.  相似文献   

17.
Abstract

Different sources of phosphorus (P) fertilizer (triple superphosphate and phosphate rocks) with different concentrations of cadmium (Cd) as an impurity were applied in different amounts to plots in a field experiment to produce soil with different concentrations of Cd. The plots were sown to canola (rape, Brassica napus L.) and spring wheat (Triticum aestivum L.). For each amount of Cd applied, the concentration of Cd in canola grain was about three times that in wheat grain. The concentration of P in canola grain was consistently about double that in wheat grain. Canola used all sources of P more effectively than wheat to produce dried shoots and grain.  相似文献   

18.
以油枯为基本原料,采用好氧堆肥方式进行堆肥试验,研究了添加4种不同微生物菌剂的条件下,油枯-稻壳-甘蔗渣堆肥体系中pH、C/N、水溶性NO3--N、水溶性NH4＋-N中的动态变化规律及菌剂对高温堆肥腐熟进程的影响。结果表明,添加菌剂能有效缩短堆肥到达高温的时间,延长高温分解阶段,加快物料水溶性NH4＋-N和C/N降低,pH和水溶性NO3--N含量升高,加快了油枯堆肥腐熟化进程。添加VT菌剂的堆肥处理相比其他在堆肥15d后最先进入高温分解阶段,高温持续时间为10d,提早5d腐熟,水溶性NO3--N含量从71.41mg.kg-1增加到887.4mg.kg-1,C/N的降低有效促进了NH4＋-N向NO3--N的转化,加快了油枯堆肥化进程,有助于提高堆肥腐熟化程度,说明添加VT菌剂的堆肥腐熟效果相对显著。  相似文献   

19.
不同地区油菜秸秆制备的生物质炭对酸性红壤的改良效果   总被引：1，自引：0，他引：1  
董颖  邵捷  徐仁扣  王辉  赵震杰  姜军 《土壤》2020,52(1):134-138
从江西鹰潭、安徽宣城、江苏南京和淮阴等4个地区收集油菜秸秆,在500℃下厌氧热解制备生物质炭,比较生物质炭的pH、盐基离子和碳酸盐含量的差异,并在20 g/kg加入量下考察其对安徽宣城pH 4.1的酸性红壤改良效果。结果表明,江西鹰潭油菜秸秆炭pH、盐基离子和碳酸盐含量最低,安徽宣城油菜秸秆炭次之,江苏淮阴和南京油菜秸秆炭的相应参数值最高。当用这4种油菜秸秆炭改良土壤酸度时,改良效果表现为江苏淮阴>江苏南京>安徽宣城>江西鹰潭,与生物质炭pH、盐基离子和碳酸盐含量一致。因此,利用秸秆生物质炭改良土壤酸度时,不仅需要考虑炭化条件和秸秆类型,作物的产地差异也需要进行考量。  相似文献   

20.
ABSTRACT

This study was conducted in a greenhouse to evaluate the root and shoot response of canola (Brassica napus L.) to salt-stress conditions and the remobilization, deposition, and input rate of sodium (Na), potassium (K), and magnesium (Mg) at different salinity levels using two canola cultivars. A salt-tolerant (‘Kristina’) cultivar and a salt-sensitive (‘Hyola 308’) cultivar were grown in nutrient solutions with 0, 50, 100, 150, and 200 mol m?3 NaCl for 7 d. The plants were harvested after 6, 12, 18, and 24 h and 3 and 7 d after salt treatment. The results indicated that increasing salinity significantly decreased shoot and root weights 7 d after treatment. Also, K content and K-Na selectivity decreased in both cultivars, but the changes in ‘Hyola 308’ were greater than in ‘Kristina.’ Electrolyte leakage was increased significantly by salinity, and cell-membrane stability of ‘Hyola 308’ was damaged more than that of ‘Kristina’. Sodium import, transport, and deposition was increased by salinity concentration but remobilization was decreased. The K and Mg import, deposition, and remobilization were also decreased. From this experiment we can conclude that greater K and Mg remobilization in ‘Kristina’ could be a mechanism of salt tolerance in canola.  相似文献   

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