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1.
以西天山野果林中的重要伴生种鞑靼忍冬和疏花蔷薇带芽茎段为外植体,研究不同培养基及激素配比对芽分化、增殖及再生的影响。结果表明:腋芽诱导的最适宜培养基为MS培养基,鞑靼忍冬和疏花蔷薇诱导率分别可达99.12%和100%;适合鞑靼忍冬腋芽增殖的培养基为MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+GA 31.0 mg·L-1,增殖系数可达5.37;而疏花蔷薇腋芽增殖的最佳培养基为MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1+GA 31.0 mg·L-1,增殖系数高达3.0;适合鞑靼忍冬组培苗生根的最佳培养基为1/2 MS+NAA 1.5 mg·L-1+6-BA 0.05 mg·L-1+活性炭1.0 g·L-1,生根率为100%;而疏花蔷薇组培苗生根最佳的培养基为1/2 MS+NAA 1.5 mg·L-1+6-BA 0.1 mg·L-1+活性炭1.0 g·L-1,生根率为66.67%。移栽到单独的珍珠岩基质中的鞑靼忍冬和疏花蔷薇组培苗成活率最高,成活率分别达90.00%和75.00%。  相似文献   

2.
本试验以红落藜为试验材料,进行茎段组织培养及植株再生的研究。试验结果表明75%酒精5s+0.1%升汞8min既可进行彻底消毒,又保证具有较高的成活率;通过初代培养筛选出NAA为最适的生长激素,最适培养基为MS + 6-BA 1.5 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%;通过继代培养筛选出最适培养基为MS + 6-BA 2.0 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%;最适的生根培养基为1/2MS + IBA1.0 mg?L-1 + NAA 0.1mg?L-1+琼脂0.8% + 蔗糖2% + 活性炭0.2%,生根率为80%。当试管苗叶片数3~5片,苗高2cm~3cm,生根数3~4条时,移栽至蛭石和腐殖土(1∶2)混合的基质中,保湿遮阴,成活率可达90%以上。  相似文献   

3.
为探究不同浓度的无机盐、蔗糖、KT、NAA和6-BA等对金毛狗脊原叶体增殖和孢子体转化的影响,采用正交设计实验方法,设置5因素4水平正交试验,筛选金毛狗脊原叶体增殖和孢子体转化的最佳培养基。结果表明,原叶体增殖最佳培养基为1/2MS+2%蔗糖+0.1 mg/L NAA+0.3 mg/L 6-BA+0.1 mg/L KT。孢子体转化最佳培养基为1/4MS+1%蔗糖+0.5 mg/L KT+0.1 mg/L NAA。  相似文献   

4.
药百合种子萌发及染色体倍性检测   总被引:2,自引:1,他引:1  
以浙江省广布种药百合为材料,采用不同激素浓度的培养基进行种子诱导及萌发实验,以不添加任何激素的MS培养基为对照,对药百合种子萌发率、诱导率、诱导芽数及生长势等进行研究,同时,对药百合种子诱导所形成试管苗进行染色体制片常规技术分析。结果表明,添加不同激素浓度培养基能显著促进药百合种子萌发,最佳培养基为MS+6-BA1.0 mg.L-1+NAA0.1 mg.L-1,萌发率为83.3%,诱导率为88.9%,平均诱导不定芽数达2.5个;未经剥皮处理的种子在30 d时仍无任何萌动;将诱导后的种子转至MS基本培养基中,仍表现出与转接前一致的生长状态;93%以上种子属于子叶出土型,仅接种在高浓度6-BA(1.0 mg.L-1)及低浓度NAA(0.1~0.2 mg.L-1)培养基上的种子有少量表现为种子留土型,占4%;种子萌发试管苗染色体倍性为2n=2x=24,未发生遗传变异。  相似文献   

5.
陈爽  李叶华  赵冰 《种子》2020,(4):60-63,70
以蜡莲绣球和莼兰绣球种子为外植体,探究不同培养基类型、不同激素浓度对其增殖及生根的影响。结果表明:蜡莲绣球最佳增殖培养基为MS+0.8 mg·L^-16-BA+0.05 mg·L^-1 NAA,增殖系数高达9,较适宜的生根培养基为1/2 MS+0.1 mg·L^-1 IBA+NAA 0.1 mg·L^-1或未加激素的1/2 MS,生根率为100%;莼兰绣球最佳增殖培养基为MS+0.8 mg·L^-16-BA+0.2 mg·L^-1 IBA,增殖系数高达9.67,较适宜的生根培养基为1/2 MS+0.2 mg·L^-1 IBA+NAA 0.5 mg·L^-1或未加激素的1/2 MS,生根率可达100%。  相似文献   

6.
孟祥玉  熊亚  张素勤  耿广东 《种子》2019,(10):14-18
以草石蚕叶片和茎段为外植体,研究植物生长调节剂对草石蚕愈伤组织和不定芽诱导的影响,筛选愈伤组织及不定芽诱导的适宜培养基配方。结果表明,茎段在MS+NAA0.6mg·L^-1+6-BA6mg·L^-1培养基上愈伤组织诱导率和干重最高,分别为96.25%和0.027g;叶片在MS+NAA0.6mg·L^-1+6-BA6mg·L^-1培养基上愈伤组织诱导率、干重和鲜重均最大,分别为97.5%、0.647g和0.132g;在MS+NAA0.1mg·L^-1+6-BA6mg·L^-1+GA33mg·L^-1培养基上愈伤组织分化不定芽率最高,为76.67%;分化芽数最多的是MS+NAA0.1mg·L^-1+6-BA4mg·L^-1+GA32mg·L^-1培养基,其芽数为13.25个。  相似文献   

7.
烤烟(Nicotiana tabacum L)嫩茎3~15层表皮和亚表皮薄细胞层在附加有适当激素配比的MS培养基上可一次培养成苗。薄细胞层一次培养成苗的适宜培养基为:MS+NAA0.1~0.3 mg/l +IBA0.1 mg/l +6-BA1.5~2.5 mg/l+3%蔗糖;BA浓度控制在2.5mg/l以下有利于培育壮苗。适宜的生根培养基为:1/2MS+2%蔗糖+NAA0.1~0.2 mg/l。  相似文献   

8.
掌叶半夏微茎尖培养脱毒和快繁体系的建立   总被引:1,自引:1,他引:0  
研究了茎尖大小、激素浓度、培养基成分对药用植物掌叶半夏脱毒效果的影响以及影响无病毒苗快速繁殖的因素等,结果表明:茎尖大小是影响茎尖成活和脱毒效果的主要因素,0.2~0.5mm的茎尖培养在MS+6-BA 1.0mg/L+NAA 0.1mg/L+蔗糖30g/L+琼脂7g/L培养基上,成活率为53.3%,病毒脱除率为76.7%,培养基中附加247mg/L NH4 +可有效提高茎尖成活率至86.7%,同种培养基上继续培养,可一次性成功诱导形成试管苗,移栽成活率高达100%。MS+6-BA 2.0mg/L+NAA 0.5mg/L+GA3 0.5mg/L组合最有利于试管茎的形成和发育,从而建立了以掌叶半夏微茎尖为外植体的脱毒和快速繁殖体系。  相似文献   

9.
驱蚊香草快繁体系的建立及工厂化育苗主要影响因素研究   总被引:2,自引:2,他引:0  
以驱蚊香草的幼嫩叶片作外植体,在含不同激素的不同培养基上进行丛生芽的诱导、芽的继代增殖、芽生根与移栽研究,以快速获得再生植株。结果表明,丛生芽诱导培养基以MS+6-BA1.0mg/L+NAA0.1mg/L较好,诱导率为96%;增殖培养基以MS(B5)+6-BA0.5mg/L +NAA0.1mg/L较好,增殖系数达8倍以上,且增殖芽生长好;壮苗培养基为MS(B5)+6-BA0.1mg/L+NAA0.2mg/L为好;生根培养基以1/2MS+NAA0.1 mg/L和MS+IBA0.2 mg/L为最佳,生根率达100%,幼苗生长势旺。影响工厂化育苗的主要因素有激素浓度、继代次数和移栽管理  相似文献   

10.
《分子植物育种》2021,19(16):5470-5474
为建立泽林考兰薄层细胞的再生体系,以其试管苗茎尖薄层细胞为外植体,研究了不同激素浓度配比对其类原球茎诱导、增殖、分化及生根的影响。结果表明,茎尖薄层细胞在1/2MS+6-BA 2.0 mg/L+TDZ2.0 mg/L+NAA 0.1mg/L培养基上的类原球茎的诱导率最高,达53.3%;类原球茎在1/2MS+6-BA 2.5 mg/L+NAA 0.1 mg/L培养基上培养40 d后,增殖系数为9.6,类原球茎在1/2MS+6-BA 0.5 mg/L+NAA 0.15 mg/L培养基上培养40 d后分化出小苗,分化率为89.7%;小苗在1/2MS+NAA 0.2 mg/L+AC 0.5 g/L培养基上生根培养40 d后,生根率达94.2%;小苗驯化移栽成活率达90%以上,长势良好。本研究通过以泽林考兰试管苗茎尖薄层细胞为外植体诱导出类原球茎,建立了泽林考兰薄层细胞再生体系,为泽林考兰试管苗产业化生产提供了新的途径。  相似文献   

11.
覆膜栽培对甘薯幼根生长发育、块根形成及产量的影响   总被引:6,自引:0,他引:6  
选用济徐23为材料,进行2年的大田试验和一年的盆栽试验,研究2种覆膜栽培对甘薯生长前期幼根生长发育和吸收能力、分化根内源激素含量和封垄期单株块根鲜重的影响及其与产量的关系。结果表明,覆黑色膜和覆透明膜处理与对照相比,均显著(P<0.05)增加甘薯秧苗栽植后10 d和20 d的幼根数量、总长度、鲜重、表面积、体积和幼根根系的吸收面积、活跃吸收面积,其中的幼根数量、鲜重、体积、幼根根系的吸收面积、活跃吸收面积在2种覆膜处理间差异显著(P<0.05),覆黑色膜优于覆透明膜;2种覆膜处理显著(P<0.05)提高秧苗栽植后10 d的根系活力,且覆黑色膜优于覆透明膜。同时,在块根分化期(秧苗栽植后20 d和30 d)2种覆膜处理显著(P<0.05)提高了分化根的ZR含量,促进初生形成层的活动和块根形成;在块根膨大初期(秧苗栽植后40 d)2种覆膜处理显著(P<0.05)提高分化根的ABA含量和显著(P<0.05)降低分化根的GA含量,促进次生形成层的活动、淀粉积累和块根膨大,其中,块根膨大初期2种覆膜处理ABA和GA含量差异显著(P<0.05),均覆黑色膜处理效果最好。在2年的大田试验中,2种覆膜处理与对照相比,均显著提高了甘薯封垄期的单株有效薯块数和单薯鲜重,覆黑色膜的单株有效薯块数高于覆透明膜,而覆透明膜的单薯鲜重显著(P<0.05)大于覆黑色膜处理。覆黑色膜和覆透明膜处理2011年分别增产10.71%和5.76%,2012年分别增产12.99%和7.45%。  相似文献   

12.
地黄栽培种与野生种内源激素含量的差异   总被引:1,自引:0,他引:1  
块根的发育和形成与植物内源激素密切相关。本研究测定地黄2个栽培种和2个野生种在生长发育过程中内源激素含量表明,地黄叶片中IAA、ZR和ABA含量变化均呈单峰曲线,即在拉线期及快速增长期逐渐升高,之后逐渐降低,但各生育期栽培种叶片中IAA、ZR和ABA含量均高于野生种;栽培种与野生种块根中IAA和ZR含量变化规律明显不同,在拉线期与快速增长期,栽培种迅速上升,而后逐渐降低,而在整个生长期野生种变化不明显;整个生育期参试品种叶片中GA含量变化不大,栽培种在快速增长期根中GA、ABA的含量迅速上升,而野生种变化不明显。从上述变化规律来看,地黄块根的形成与发育是多种内源激素协同作用的结果。IAA和ZR是块根起始分化关键激素,决定了块根的形成,GA、ABA在地黄块根的发育与膨大中起了更重要的作用。  相似文献   

13.
Law of Endogenous Hormone Variation of Growing Tobacco Root   总被引:5,自引:1,他引:5  
采用盆栽实验研究了烟草根系生长过程中根系和叶片中生长素,细胞分裂素,赤霉素及过氧化物酶,多酚氧化酶的分布规律,并分析了烟根内3种内源激素含量和两种酶活性的相关性,初步阐明了内源激素对烟草生根的诱导原理。  相似文献   

14.
In order to explore the changes of endogenous hormones on storage root formation and its relationship with storage root number under different potassium application rates of sweet potato root, sweet potato varieties ‘Yanshu 25’ and ‘Beijing 553’ with significant differences in storage root number were used as experimental materials, potassium oxide (K2O) was used as fertilizer, and four potassium fertilizer gradients of 0 (K0), 120 (K2), 240 (K3), and 360 kg hm-2 (K4) were set. The effects of different potassium application rates on the contents of endogenous hormones, the activity of related metabolic enzymes, the number of storage root and root evenness in swelling roots of sweet potato during root formation and harvest stage were studied. Compared with the treatment without potassium fertilizer (K0), the application of potassium fertilizer decreased the enzyme activities of indoleacetic acid oxidase (IAAO) and peroxidase (POD), increased the content of indole-3-acetic acid (IAA), increased the content of zeatin riboside (ZR) and decreased the content of gibberellins (GA3). Potassium application enhanced the activity of primary cambium and promoted the differentiation from adventitious root to storage root by regulating the content of endogenous hormones in swelling roots of sweet potato. Compared with the control, the application of potash fertilizer significantly increased the number and weight of storage roots per plant and root yield of ‘Yanshu 25’ and ‘Beijing 553’. The number of storage roots per plant of ‘Yanshu 25’ and ‘Beijing 553’ increased by 3.16%-25.40% and 3.85%-33.11%, respectively, and the yield increased by 4.22%-17.31% and 3.94%-18.45%, respectively. Compared with the potassium application treatments of the two varieties, the K2 treatment had the highest storage roots number per plant, the highest weight and yield of storage roots, with the best root evenness.  相似文献   

15.
木薯内源激素含量与块根淀粉积累关系研究   总被引:2,自引:1,他引:1  
为探讨木薯内源激素(IAA、ABA、GA3、CYT)含量和块根淀粉积累的关系,分别对高低淀粉木薯品种不同时期块根、茎秆、叶片内源激素(IAA、ABA、GA3、CYT)和块根淀粉含量变化进行研究。结果表明:块根、茎秆、叶片中IAA含量变化趋势一致,在块根形成期达到最大值;块根、茎秆内ABA、GA3,叶片内ABA与块根淀粉含量呈极显著正相关关系,其中茎秆ABA、GA3主要是通过影响光合产物的装载和运输来促进块根淀粉的积累;在块根形成期和膨大期,高淀粉木薯品种‘辐选01’块根内CYT含量高且持续时间长。叶片中IAA、CYT、GA3与块根淀粉含量呈极显著负相关关系,随着块根成熟,这3种内源激素都有不同程度的上升趋势,可能与它抗衰老的能力有关。在块根膨大期,‘华南124’茎秆GA3含量极显著高于‘辐选01’,‘辐选01’块根、叶片ABA含量在生长后期显著高于‘华南124’,说明低淀粉木薯品种后期不易早衰,但地上部分生长过旺不利于块根淀粉积累。  相似文献   

16.
选用8个木薯品种(系)调查和评价储藏根采后3、7、12、17和27 d贮藏保鲜过程,发现其耐贮存性从高至低依次为SMH、BRA755、SC205、SC5、BRA440、7G-2、T1和BRA258。利用线性回归分析表明,木薯储藏根贮存变质抗性与干物质含量呈负相关,相关系数r为0.687 (P=0.05);与淀粉率含量呈负相关,相关系数r为0.696 (P=0.05);与储藏根氢氰酸(HCN)含量无相关性。其中SMH贮存变质抗性最强,储藏根干物质和淀粉含量最低;BRA258和T1贮存变质抗性最差,干物质和淀粉含量最高,因此这3个木薯品种(系)在育种上具有较高的利用价值。  相似文献   

17.
木麻黄自毒作用物对其幼苗内源激素的影响   总被引:3,自引:0,他引:3  
林武星 《中国农学通报》2009,25(19):100-103
以15年生木麻黄根系的水浸液作为他感作用物,采用水培方法研究木麻黄自毒作用对其苗木内源激素的影响。结果表明,木麻黄自毒作用主要表现在对其幼苗叶、根中生长类激素IAA、GR、iPAs和ZRs含量下降,且随自毒作用物质浓度升高下降幅度增大,ABA含量、ABA/IAA、ABA/GA、ABA/iPAs、ABA/ZRs、ABA/IAA+GA+iPAs+ZRs增加,内源激素失衡。木麻黄自毒作用对其根、茎和叶生长量和生物量下降与IAA、GR、iPAs和ZRs含量减少和ABA含量增加及内源激素失衡有密切关系。  相似文献   

18.
Cassava is predominantly an upland crop that is also cultivated in inland valley swamps (IVS). Identifying physiological traits that can withstand excess moisture stress can aid in the selection and use of stable cassava cultivars in IVS. Three cassava cultivars were evaluated for growth and yield in the upland and IVS ecologies in the 1993–94 crop seasons using a randomized complete block design. In the upland, 80/40 outyielded 87/29 and 'coco', while in the IVS, 87/29 had the highest yield. Highest yielding cultivars in each ecology also had the highest tuberous root bulking rate (TBR), dry matter (DM) production, crop growth rate (CGR), relative growth rate, net assimilation rate (NAR), leaf area index, leaf production rate and leaf life. Positive correlations between these parameters and tuberous root yield were noted. High yield of 87/29 in IVS was partly due to its low tuberous root rotting. High root rotting in 80/40 was partly as a result of the greater depth of tuberous roots in the soil (0.3–0.6 m) as compared to the other cultivars (0.15–0.3 m) where roots were in contact with stressful water levels earlier than others. The yield and yield components, growth and leaf characters were all drastically reduced when the cultivars were grown in IVS as compared to upland. For example, root yields of 'coco', 87/29 and 80/40 were reduced by 53 %, 60 % and 92 %, respectively, in IVS. Selection of cassava cultivars with longer leaf life and leaf area maintenance leading to high CGR, TBR, NAR and harvest index (in IVS and upland), coupled with a shallow tuberous root formation zone (in IVS) can give higher root and leaf yields in the two ecologies.  相似文献   

19.
Solutions of sucrose, glucose and fructose were artificially injected into the stems of sweet potato plants. The effects of solution injection on both dry matter production and the activity of adenosine 5'-diphosphate glucose pyrophosphorylase (AGPase) in tuberous roots were investigated and compared. The total weight of carbon (TC) artificially and photosynthetically supplied to a plant during the treatment period of 40 days was 0.987–1.869 times the weight of photosynthetically assimilated carbon alone. At the final sampling time, the dry matter weight of tuberous roots in the plants injected with sugar solutions showed a 2.73–9.13-fold increase over that of the control plant. The root weight linearly increased with TC. The activity of AGPase was also enhanced by solution injections, with 27–63 % increases compared to the control, but was not significantly related to TC. The injection of sugar solutions is concluded to have a dual effect on root production in sweet potato. One effect is that the increased sugar concentration in the plant increases AGPase and sink activities, and the other effect is that the increased carbon supply quantitatively promotes starch synthesis and accumulation in roots.  相似文献   

20.
华北五角枫‘京2’插穗生根过程中内源激素变化   总被引:1,自引:1,他引:0  
为建立高效的扦插技术体系,运用酶联免疫吸附测定法,测定了华北五角枫‘京2’扦插过程中插穗内生长素(IAA)、脱落酸(ABA)、赤霉素(GA)、玉米素(ZR)4种植物内源激素的含量,研究了华北五角枫‘京2’扦插生根的生理生化机理。结果表明:华北五角枫‘京2’扦插生根过程中插穗内源激素含量与生根密切相关,插穗根原基分化形成及生长发育在高浓度的内源IAA及低浓度的内源ABA作用下被促进,华北五角枫‘京2’插条不定根的形成在高浓度的内源GA作用下会被抑制;华北五角枫‘京2’扦插生根的不同时期,其内源ZR的作用不同,低浓度的内源ZR促进根原基分化形成,而较高浓度的内源ZR有利于根原基生长发育;4种内源激素的初始含量与生根率的相关性表现出不同,ABA、GA的初始含量与生根率呈负相关,而IAA、ZR呈正相关,其中IAA与生根率关系最密切。  相似文献   

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