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1.
水稻生育期内红壤稻田氨氧化微生物数量和硝化势的变化 总被引：1，自引：1，他引：0
利用荧光定量PCR(Real-timePCR)技术,通过特异引物检测amoA基因拷贝数分析了水稻不同生育期红壤稻田土壤中氨氧化细菌(Ammonia oxidizing bacteria,AOB)和氨氧化古菌(Ammonia oxidizing archaea,AOA)的数量变化,并测定了土壤潜在硝化势。结果显示:红壤稻田土壤中AOA数量显著高于AOB,二者比例在1.6～120.7之间;红壤稻田根层土中AOA数量显著高于表土,随水稻生长根层和表土中AOA数量均逐渐增加,且根层土中增加幅度更大;在水稻生长前期表土中AOB数量较多,孕穗期后根层土中AOB数量显著增加且高于表土。水稻生长期内土壤潜在硝化势也具有逐渐增加趋势,且根层土潜在硝化势增加幅度更大。根层土中潜在硝化势与AOB和AOA数量均呈显著正相关,而表土中潜在硝化势只与AOA数量存在显著正相关。研究表明,红壤稻田土壤中AOA数量更为丰富,且与硝化作用的关联程度更为密切,证实了氨氧化微生物在红壤稻田土壤微生物组成及其生态系统功能中的重要性。 相似文献
2.
氮肥对稻田土壤反硝化细菌群落结构和丰度的影响 总被引：6，自引：1，他引：5
以氮肥田间定位试验为研究对象,利用PCR-DGGE(聚合酶链反应变性梯度凝胶电泳)和荧光定量PCR(real-time PCR)技术,通过对反硝化细菌nirS基因的检测,分析了定位试验第2年稻田反硝化细菌群落结构和丰度的变化。DGGE图谱及依据其条带位置和亮度数字化数值进行的主成分分析(PCA)结果均显示:在氮肥定位试验第2年,与不施肥对照(CK)比较,在水稻各个生育期(分蘖期、齐穗期和成熟期)内,施用氮肥[150kg(N)·hm-2]的稻田根层土或表土中的反硝化细菌群落结构均无明显变化;且稻田根层土或表土中的反硝化细菌群落结构在水稻各个生育期间也均无明显差异。荧光定量PCR结果显示,在水稻生长发育过程中,施用氮肥的稻田根层土或表土中的反硝化细菌nirS基因拷贝数始终显著(P<0.05)高于其对应的不施肥对照。此外,无论施用氮肥与否,根层土中的反硝化细菌nirS基因拷贝数在水稻成熟期时都会显著(P<0.05)降低;但表土中的nirS基因拷贝数在水稻各生育期间无明显变化;且水稻成熟期时施用氮肥和不施肥的稻田表土中nirS基因拷贝数都显著(P<0.05)高于根层土。同时,与对照比较施用氮肥可促进水稻增产44%。研究表明,短期定位试验中施用氮肥能够显著提高稻田土壤反硝化细菌的丰度,但对其群落结构没有明显影响。 相似文献
3.
模拟稻秸原位还田条件,分别在水稻土和红壤中添加水稻秸秆培养70d ,第0、5、2 5、4 5、70天采集土样。采用非机械破壁法直接提取水稻土和红壤细菌总DNA ,水稻土细菌总DNA经过二次纯化;红壤细菌总DNA经过一次纯化后,PCR扩增其16SrDNAV3可变区,均可获得清晰的目的条带,对扩增产物进行DGGE分析,结果显示:水稻土和红壤样品的DGGE条带增加,说明稻秸能够增加土壤细菌群落分子多态性的丰富度,随着培养期的延长,施有稻秸的处理中土壤细菌群落多态性的变化远远复杂于空白对照土壤中的细菌群落变化;同时发现在稻秸刺激下不同土壤细菌群落多态性高峰期出现时间不同 相似文献
4.
有机肥和无机肥对水稻土产甲烷的影响 总被引：3，自引：0，他引：3
在盆栽条件下,研究了二种有机肥和五种无机肥对淹水稻上产甲烷细菌数量和甲烷释放量的影响。结果表明有机肥对产甲烷菌数量和甲烷释放量均有较大的促进作用,其中以绿肥最为显著。无机肥料的施用处理中,NH4HCO3、（NH4）2SO4、CO（NH2）2和NH4Cl对水稻土产甲烷细菌数量的增加和甲烷释放量均略有促进作用,而施NaNO3处理的对甲烷释放总量有较明显的抑制作用。 相似文献
5.
华南地区覆膜旱种稻田甲烷排放及其与土壤水分和温度的关系 总被引：2，自引：0，他引：2
采用静态箱法和田间小区试验,研究了常规稻田和覆膜旱种稻田水稻全生育期CH4的排放规律,探讨了温度和水分与稻田CH4排放的关系。结果表明:覆膜旱种稻田的甲烷排放量明显低于常规水田的排放量,常规水田的甲烷累计排放通量为20.38g/m2,覆膜旱种稻田为2.46g/m2,水稻覆膜旱种后甲烷排放量降低了88%。常规水田CH4排放峰期持续了35d,覆膜旱种稻田CH4排放峰期为25d,两者在CH4排放高峰期的排放量分别占整个生育期累计排放量的72%和97%。覆膜旱作稻田CH4排放量降低,主要表现在最大排放峰值降低和排放峰持续时间缩短。土壤温度(5cm处)和水分与水稻生育期稻田甲烷的排放有显著正相关。CH4排放通量大于1.0mg·m-2·h-1主要集中在土壤质量含水率高于36.25%的区域,在土壤质量含水率小于36.25%时,常规稻田和覆膜旱种稻田都只有少量CH4排放。 相似文献
6.
稻田水分管理和秸秆还田对甲烷排放的微生物影响 总被引：2，自引：0，他引：2
为了探讨不同水分管理和秸秆还田对稻田甲烷排放的影响机理,依托中国科学院亚热带农业生态研究所长沙农业环境监测研究站长期定位试验,选择长期淹水(CF)、秸秆还田+长期淹水(HS+CF)、常规灌溉(IF)、秸秆还田+常规灌溉(HS+IF)4个处理,应用限制性片段长度多态技术(T-RFLP)和实时荧光定量PCR技术在DNA和c DNA水平对比研究不同水分管理和秸秆还田影响稻田土壤甲烷排放的微生物分子机理。结果表明:长期淹水处理的甲烷排放量显著高于常规灌溉处理,秸秆还田处理对甲烷排放的影响不显著。产甲烷菌和甲烷氧化菌群落结构只对土壤水分管理产生响应,对秸秆还田没有响应。水分管理和秸秆还田显著影响产甲烷菌的数量,却对甲烷氧化菌数量没有影响。产甲烷菌和甲烷氧化菌群落组成和表达群落组成存在明显的分异,c DNA水平上产甲烷菌和甲烷氧化菌表达群落组成对水分管理的响应比DNA水平群落组成更敏感。稻田甲烷排放通量与mcr A和pmo A基因丰度和表达丰度均没有显著相关性,只与DNA水平基因丰度比值(mcr A/pmo A)呈显著正相关关系。可以看出,长期淹水处理可以通过改变土壤中产甲烷菌和甲烷氧化菌的群落结构和数量及两者数量的比例来调控甲烷的排放量。 相似文献
7.
Naoki Harada Masaya Nishiyama Shigeto Otsuka Satoshi Matsumoto 《Soil Science and Plant Nutrition》2005,51(3):361-367
Phototrophic purple bacteria (PPB) are one group of dinitrogen (N2 )-fixing bacteria often found in the anoxic and photic zone of paddy soil. In the present study, the effects of inoculation of PPB on grain yield of rice and changes in their populations and nitrogenase activity were investigated with and without surface application of rice straw in a pot experiment. As an inoculant, Rhodopseudomonas palustris strain KN122 isolated from a paddy soil was selected, and the cell suspension was inoculated into the floodwater once or three times during the cultivation. As a result, the inoculation of PPB increased the grain yield of rice. Compared to the control, the grain yield was 9% higher in the inoculated plot without rice straw application. The treatment was more effective in combination with rice straw application. In the plots where PPB were inoculated with rice straw, grain yields were 21% (single inoculation) and 29% (triple inoculation) higher. Populations of PPB in the plots without rice straw application significantly increased by the inoculation, unlike those in the plots with rice straw application, except for the bulk soil. Phototrophic and heterotrophic nitrogenase activities (acetylene-reducing activity) associated with the soils or residues of the rice straw were not affected by PPB inoculation throughout the experiment. This indicates that the inoculation of PPB into floodwater may not be effective for enhancing N2 fixation in paddy soils and that the beneficial effect of PPB inoculation on the grain yield of rice may be due to unidentified functions of PPB other than biological N2 fixation. 相似文献
8.
不同土壤肥力条件下水稻控释氮肥效应及其氮素利用的研究 总被引：23，自引：2，他引：23
田间试验在湖南的衡山县和长沙县进行,探讨了不同肥力水平水稻土上水稻控释氮肥对早、晚稻的增产效果和肥料氮利用率。结果表明,在肥力水平较低的水稻土上施用水稻控释氮肥对水稻的增产效果极为显著,应当增加施用量;在肥力水平较高的水稻土上,水稻吸收土壤氮较多,肥料氮的吸收量与增产作用自然减少,应适当减少施用量。不同肥力水平的土壤上水稻控释氮肥较等氮量尿素增产4.4%～16.4%。较无氮区增产13.8%～74.5%。水稻控释氮肥在不同肥力水平土壤中早、晚稻的利用率为69.7%～86.9%,平均比等N量尿素高37.6%,且水稻控释氮素利用率的高低与土壤肥力水平有关。水稻控释氮肥作一次性全量基肥施用,氮素生产效率明显高于尿素,是增产的主要原因。 相似文献
9.
Allelopathic rice can release allelochemicals from roots to inhibit neighboring plant species, but little is currently known about their fate and impact on microorganisms in paddy soil. This study showed that allelopathic rice PI312777 released much higher concentrations of allelochemical (5,7,4'-trihydroxy-3',5'-dimethoxyflavone) than non-allelopathic rice Liaojing-9 in field. When quantitative 5,7,4'-trihydroxy-3',5'-dimethoxyflavone was added into soil, flavone gave a short half-life of 18.27 +/- 2.32 h (r(2) = 0.94) and could easily be degraded into benzoic acid. Benzoic acid with a half-life of 29.99 +/- 2.19 h (r(2) = 0.96) was more resistant toward degradation in paddy soil. Furthermore, both the culturable microbial population and the entire microbial community structure of soil incubated with flavone and benzoic acid were evaluated using the soil dilution plate method and phospholipid fatty acid (PLFA) analysis, respectively. It appeared from the results that flavone could reduce microorganisms especially for fungi present in paddy soil, while benzoic acid could induce a higher response for soil microorganisms especially for bacteria. Consequently, flavone would be responsible for the dynamics of soil microorganisms during the early period, and any observed effect during the late period would be very likely due to its degradation product benzoic acid rather than flavone itself. These results suggested that allelopathic rice varieties could modify soil microorganisms to their advantage through the release of allelochemicals. The concentration and fate of discriminating 5,7,4'-trihydroxy-3',5'-dimethoxyflavone between allelopathic and non-alleloparhic varieties tested in rice soil would result in the different patterns of microbial population and community structure in paddy ecosystems. 相似文献
10.
Degradability of black carbon and its impact on trace gas fluxes and carbon turnover in paddy soils 总被引：2，自引：0，他引：2
Christian Knoblauch Arina-Ann Maarifat Stephan M. Haefele 《Soil biology & biochemistry》2011,43(9):1768-1778
Rice paddy soils are characterized by anoxic conditions, anaerobic carbon turnover, and significant emissions of the greenhouse gas methane. A main source for soil organic matter in paddy fields is the rice crop residue that is returned to fields if not burned. We investigated as an alternative treatment the amendment of rice paddies with rice residues that have been charred to black carbon. This treatment might avoid various negative side effects of traditional rice residue treatments. Although charred biomass is seen as almost recalcitrant, its impact on trace gas (CO2, CH4) production and emissions in paddy fields has not been studied. We quantified the degradation of black carbon produced from rice husks in four wetland soils in laboratory incubations. In two of the studied soils the addition of carbonised rice husks resulted in a transient increase in carbon mineralisation rates in comparison to control soils without organic matter addition. After almost three years, between 4.4% and 8.5% of the black carbon added was mineralised to CO2 under aerobic and anaerobic conditions, respectively. The addition of untreated rice husks resulted in a strong increase in carbon mineralisation rates and in the same time period 77%-100% of the added rice husks were mineralised aerobically and 31%-54% anaerobically. The 13C-signatures of respired CO2 gave a direct indication of black carbon mineralisation to CO2. In field trials we quantified the impact of rice husk black carbon or untreated rice husks on soil respiration and methane emissions. The application of black carbon had no significant effect on soil respiration but significantly enhanced methane emissions in the first rice crop season. The additional methane released accounted for only 0.14% of black carbon added. If the same amount of organic carbon was added as untreated rice husks, 34% of the applied carbon was released as CO2 and methane in the first season. Furthermore, the addition of fresh harvest residues to paddy fields resulted in a disproportionally high increase in methane emissions. Estimating the carbon budget of the different rice crop residue treatments indicated that charring of rice residues and adding the obtained black carbon to paddy fields instead of incorporating untreated harvest residues may reduce field methane emissions by as much as 80%. Hence, the production of black carbon from rice harvest residues could be a powerful strategy for mitigating greenhouse gas emissions from rice fields. 相似文献