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1.
1.?This research was conducted to determine the impact of diet supplementation with yeast cell walls and Yucca schidigera extract on the growth performance, antibody titres, and intestinal tissue histology of layer chicks.

2.?White, 1-d-old, Hy-Line hybrid chicks (n?=?840) were divided into 4 main groups, each comprising 7 replicates of 30 chicks (n?=?210): (1) control; (2) 1000?mg/kg yeast cell walls (YCW) added; (3) 1000?mg/kg Yucca schidigera extract (YE) added; and (4) 500?mg/kg YE?+?500?mg/kg YCW added. The trial lasted 60?d.

3.?Daily weight gain of the chicks was positively affected between d 45-60 in the YE and YCW?+?YE groups compared with the control group.

4.?Overall, feed consumption did not differ between the control and YCW, YE, YCW?+?YE groups during the 60?d study period. Feed efficiency was better in the YE and YCW?+?YE groups than in the control group between d 1-60.

5.?During the 60?d evaluation period, live weight gain, and final live weight were higher in YE and YCW?+?YE groups than in the control group.

6.?Antibody titres against infectious bronchitis and infectious bursal disease did not differ among the 4 treatments, but those for Newcastle disease were higher in the YE?+?YCW groups than in the control, YCW and YE groups on d 45.

7.?There were differences in intestinal histomorphometry between the 4 treatments. The height of the jejunal and ileal villi was greater in the YE and YCW?+?YE groups than in the control and YCW groups

8.?It can be concluded that YCW and YE supplementation for layer chicks is beneficial for growth performance and intestinal histology during the 1-60?d growing period.  相似文献   


2.
1.?Using the so-called TOBEC (Total Body Electrical Conductivity) method, which allows the determination of egg composition in vivo, correlations between egg composition, hatchability and hatched chicks’ development were studied.

2.?A total of 1500 hen eggs (Ross-308) were measured by TOBEC, and eggs with extremely high (10%, n = 150), extremely low (10%, n = 150) and average (10%, n = 150) electrical conductivity values were chosen for further investigation.

3.?During the incubation period, it was observed that eggs with high electrical conductivity had a significantly higher mortality than eggs with low electrical conductivity.

4.?It was observed that both the increase in electrical conductivity at the same egg weight, and the increase in egg weight at the same electrical conductivity resulted in an increase in the hatching weight.

5.?It was found that the dry matter, protein and fat content of the chicks hatched from eggs with low electrical conductivity was higher at hatching than that of the chicks hatched from eggs with high electrical conductivity.

6.?At 42 d of age the liveweight of cocks and pullets hatched from eggs with low electrical conductivity was 3·2 and 8·2% higher than the liveweight of cocks and pullets hatched from eggs with high electrical conductivity.

7.?Because of the higher liveweight at slaughter, there was a significant superiority of the chicks hatched from eggs with low electrical conductivity in the case of the examined carcase traits at slaughter.

8.?Similar tendencies were found also in the ratios of carcase variables to liveweight, but the between group differences were not statistically significant in this case.

9.?Based on the results it was concluded that TOBEC seems to be a useful method for separating eggs with different composition.

10.?This could be a good starting point for further in vivo investigations in order to clarify the effect of egg composition on hatchability and further development.  相似文献   


3.
1.?The objectives were to quantify the presence of neurogenic inflammation in 4 regions of the upper digestive tract of anaesthetised ducks (post-pharynx, pseudo-crop, transition between the pseudo-crop and the proventriculus, and proventriculus) after application of HCl stimulation of up to 4?M in the pseudo-crop.

2.?The second objective was to quantify the presence of neurogenic inflammation in the same digestive tract regions as mentioned above during 4 feeding periods of foie gras production (rearing, preparation to force-feeding, and second and last meals of the force-feeding period).

3.?Extravasation increased above a HCl stimulation threshold of 2?M. Furthermore, more extravasation was observed in the proventriculus compared to the other regions (P?

4.?Highest extravasation responses were observed in the proventriculus and the pseudo-crop at the end of the preparation period, and in the proventriculus after the second forced meal, compared with the rearing period (P?

5.?Such a kinetic could be indicative of a relative mildness of the irritant components associated with this feeding practice.  相似文献   


4.
1.?The purpose of this study was to investigate the effects of epidermal growth factor (EGF) on progesterone (P4) secretion from ovarian granulosa cells in Tsaiya ducks (Anas platyrhynchos var. domestica).

2.?We obtained the largest (F1) follicle from Tsaiya duck, the granulosa layer was separated and the cells were isolated according to their proximity to the germinal disc.

3.?The granulosa cells were cultured in vitro, the culture media and the cells were used to determine P4 and steroidogenic enzyme concentrations, respectively.

4.?P4 concentrations were decreased in cultured granulosa cells taken proximal to the germinal disc (GD) compared to those distal to the germinal disc (NGD).

5.?EGF inhibited both basal and ovine luteinising hormone (oLH)-induced P4 concentrations. It also inhibited the P4 secretion via protein kinase A (PKA) pathway when cultured with GD and NGD granulose cells (mixed together) in vitro.

6.?Western blot results showed decreased concentrations of cytochrome P450 side-chain cleavage (P450scc) enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD) enzyme and steroid acute regulatory (StAR) protein expression, when the cells were co-treated with EGF and oLH.

7.?The inhibitory effect of oLH-induced P4 production was attenuated by EGF by the addition of MAP-erk kinase (MEK) inhibitor (PD98059) suggests EGF may inhibit P4 production by affecting via the mitogen-activated protein kinase (MAPK) pathway.  相似文献   


5.
1.?In this study the effect of a blend of essential oils (EO) comprising 15 g/tonne thymol and 5 g/tonne cinnamaldehyde on the performance and intestinal microbiota of broilers was investigated.

2.?A total of 720 male Ross broilers were divided into two dietary treatments with 12 replicate pens per treatment. Broilers were given a control soybean-wheat-based diet with or without added EO in two diet phases (0–21 d and 22–42 d).

3.?The blend of EO increased body weight gain of broilers from 0 to 42 d by 4?5%.

4.?Caecal microbiota were affected by the EO blend; in particular increases in the proportions of Lactobacillus and Escherichia coli at 41 d was observed.

5.?The EO blend had major effects on caecal metabolites. The proportion of caecal butyrate at 20 and 41 d of age increased, whereas the proportion of caecal acetic acid at 20 d, and propionic acid and isovaleric acid at 41 d, decreased with the EO blend. In addition, the caecal proportion of spermine increased and tyramine decreased at 41 d of age with the EO treatment.

6.?The present study shows that EO supplementation exerts a positive effect on intestinal microbiota with a concomitant enhancement in growth performance. The study suggests that modulation of broiler gut microbiota composition and activity through the administration of EO offers an effective means for improving broiler performance.  相似文献   


6.
1.?The effect of dietary pea and addition of organic acid blend (OA) or probiotic (Pro) on performance and caecal microbial ecology of broiler chickens was studied.

2.?A growth trial was conducted with 160 Ross 308 female broilers from d 1 to 35 of age. There were 8 treatment groups based on either control (S) or white pea (P). Both S and P were supplemented with OA (Galliacid – fumaric acid, calcium formate, calcium propionate and potassium sorbate coated with plant triglycerides, Vetagro) and or with Pro (LABYuc-Probio – lactic acid bacteria, Saccharomyces cerevisiae and Yucca schidigeri extract, Mifarmex GmbH).

3.?Inclusion of peas in the diet increased feed intake and decreased gain:feed ratio in comparison to the control diet. Neither probiotic nor OA supplementations affected broiler performance.

4.?The caecal microbiota was characterised in 37-d-old birds by fluorescent in situ hybridisation (FISH) and terminal-restriction fragment length polymorphism (T-RFLP). Total bacterial counts in caecal contents were slightly higher for birds fed the pea diets, but were not affected by OA or Pro supplements.

5.?Neither pea nor Pro affected the Lactobacillus/Enterococcus and Streptococcus/Lactococcus counts in caecal contents, whereas OA supplementation slightly increased the Lactobacillus/Enterococcus counts. The composition of the Lactobacillus/Enterococcus population was altered by inclusion of peas as revealed by the T-RFLP patterns.

6.?The DNA fingerprint further suggested that the caecal microbiota was dominated by the lactic acid bacterium Streptococcus alactolyticus.

7.?In ileal contents, the concentration of short-chain fatty acids (SCFA) was decreased only by Pro supplementation. In caecal contents, the SCFA concentration was higher for birds fed on the pea diets, and increased significantly with Pro supplementation

8.?In conclusion, the results indicate that the use of pea and probiotics in broiler feed may stimulate the caecal commensal microbiota (growth and/or activity) to some extent and hence prevent establishment of pathogenic and zoonotic enterobacteria in these segments of the gut.  相似文献   


7.
1.?Forty-six flocks of commercially-reared Pekin ducks were studied in 23 houses differing in their ventilation and brooding systems, and water and feed resources, in order to identify factors affecting duck welfare in commercial practice.

2.?A wide range of environmental variables were measured, together with the physical and plumage condition of the ducks at two ages, whilst companies supplied mortality and growth rate data.

3.?At 23 d, more than 98% of ducks had clean eyes, nostrils and feathers and an upright posture, and 86% had no gait abnormalities. By 41 d, body condition had deteriorated slightly with 84% of ducks having clean eyes, 67% clean feathers and 79% no gait abnormalities.

4.?Gait worsened with increasing temperature and litter moisture, and atmospheric ammonia concentrations. The incidence of foot pad lesions was 10% (moderate) and 3% (severe) and was positively correlated with increasing humidity and ammonia.

5.?Average mortality rates were 5?2% for ducks reared to 3?35 kg at 48 d with average growth rates of 60?3 to 81?3 g/d. High temperatures correlated with high mortality and reduced growth rate; growth rate was not related to poor gait.

6.?Controlling the ducks’ environment, particularly temperature, humidity, litter moisture and ammonia is crucial to duck welfare. Effective ventilation systems, high quality straw and access to some form of open water were considered important for duck welfare.  相似文献   


8.
1.?The effects of injecting threonine in ovo on early growth, some immunological responses and the activity of digestive enzymes of broiler chicks were investigated. Fertile eggs were distributed into 6 groups, each of 60. These were: untreated control, sham control, 10, 20, 30 or 40 mg threonine. Threonine was dissolved in 0·5 ml sterile saline and inoculated into the yolk sac of the 14-d-old embryo through the narrow end of the egg.

2.?The ratio of chick to egg weight was 1·6% higher in the group given 30 mg threonine and at 28 d of age chicks receiving threonine were 29 to 79 g heavier than untreated controls.

3.?Food conversion ratio until 7 d after hatching was improved in those chicks receiving 10, 20 or 40 mg threonine but there was no significant effect on the activities of amylase, pepsin or trypsin.

4.?The humoral response to sheep red blood cells was significantly greater in those groups receiving 10, 20 or 30 mg threonine supplementation than in untreated controls.

5.?The response to phytohaemagglutinin-P, a measure of the cell-mediated immune response, was not affected, however.

6.?It is concluded that injections of 20 to 30 mg threonine into yolk sac can improve post-hatching growth and humoral responses of broiler chicks.  相似文献   


9.
1.?Litter samples were collected at the end of the production cycle from spread litter in a single shed from each of 28 farms distributed across the three Eastern seaboard States of Australia.

2.?The geometric mean for Salmonella was 44 Most Probable Number (MPN)/g for the 20 positive samples. Five samples were between 100 and 1000 MPN/g and one at 105 MPN/g, indicating a range of factors are contributing to these varying loads of this organism in litter.

3.?The geometric mean for Campylobacter was 30 MPN/g for the 10 positive samples, with 7 of these samples being <100 MPN/g. The low prevalence and incidence of Campylobacter were possibly due to the rapid die-off of this organism.

4.?E. coli values were markedly higher than the two key pathogens (geometric mean 2?0 × 105 colony forming units (cfu)/g) with overall values being more or less within the same range across all samples in the trial, suggesting a uniform contribution pattern of these organisms in litter.

5.?Listeria monocytogenes was absent in all samples and this organism appears not to be an issue in litter.

6.?The dominant (70% of the isolates) Salmonella serovar was S. Sofia (a common serovar isolated from chickens in Australia) and was isolated across all regions. Other major serovars were S. Virchow and S. Chester (at 10%) and S. Bovismorbificans and S. Infantis (at 8%) with these serovars demonstrating a spatial distribution across the major regions tested.

7.?There is potential to re-use litter in the environment depending on end use and the support of relevant application practices and guidelines.  相似文献   


10.
1.?Effects of early feeding with a diet containing added putrescine on duck intestinal development and growth performance were examined by a 2 × 2 factorial arrangement with two different feeding times (6 and 48 h) and two levels of putrescine (0 and 0?25%).

2.?A significant main effect of early feeding on increasing body weight (BW) was observed from hatch to 35 d, whereas dietary putrescine had no significant effect on BW.

3.?In the first week posthatch, enhanced small intestinal weight and intestinal density (weight of intestinal tissue/unit length of intestine), increased villus length and reduced crypt depth were observed in the early feeding group, while no effect was observed when putrescine was added to the diet.

4.?Maltase and sucrase activity and protein/DNA ratio in jejunum were increased by early feeding in the first week, while decreased by putrescine supplementation.

5.?In conclusion, early feeding methods have great potential for small intestine development and thereafter enhanced the growth performance of ducks, but dietary putrescine used during this period should be used cautiously to avoid toxicity.  相似文献   


11.
1.?Combinations of chromium and copper were added to the diet to assess their effects on broiler meat characteristics.

2.?For this purpose 175 one-day-old broiler chicks were divided into 7 equal groups and were given treatment feeds containing copper sulphate, chromium chloride and nicotinic acid in different combinations.

3.?The study was carried out for 5 weeks and samples were collected at the end of 15, 29 and 35 d of treatment and at 42 d after a withdrawal period of one week.

4.?Cholesterol content had decreased significantly in breast meat at d 29 in all treatment groups. In thigh meat, it decreased significantly at d 29 in groups receiving two concentrations of chromium + two concentrations of copper. Cholesterol content remained lower even after withholding the treatment for one week.

5.?Crude fat content decreased significantly in breast meat in all treatment groups. In thigh meat, at d 29, a significant reduction in crude fat was observed only in birds receiving low chromium and high copper.

6.?Crude protein at d 29 increased significantly in breast meat of birds receiving low chromium and high copper, and low or high chromium, while it decreased significantly in treatment groups after withholding the treatment. In thigh meat, at d 29, it increased significantly in treatment groups but decreased significantly after withholding the treatment.

7.?It was concluded that chromium and copper, along with nicotinic acid, have modulating effects on broiler meat under tropical conditions.  相似文献   


12.
1.?Plasma lipids were investigated to determine whether they influence the biological activities of specific coagulation proteins Factors V, VII and X.

2.?Factor activities decreased when lipids were depleted from the plasma of Single Comb White Leghorn (SCWL) and Fatty Liver Haemorrhagic Syndrome-susceptible (FLHS) laying hens.

3.?Addition of lipids removed from SCWL laying hens and FLHS-susceptible laying hens into lipid-depleted plasma of both bird strains caused an increase and decrease, respectively, in Factors V and X activities.

4.?Omega-3 fatty acids were negatively correlated to Factors V, VII and X activities. When bird strain was considered, it was significant for Factor X in SCWL laying hens. Omega-6 fatty acids were positively correlated with Factors VII and X for FLHS-susceptible laying hens.

5.?The results suggest that the type of fatty acid in plasma phospholipids influences the activities of Factors V, VII and X and by altering lipid composition in the plasma, activities of coagulation factors may be affected.  相似文献   


13.
1.?The aim of the present study was to demonstrate trace mineral interactions among organic copper, iron, manganese and zinc (Cu, Fe, Mn and Zn) in broiler chickens.

2.?Three experiments were conducted using a control diet which was deficient in Cu, Fe, Mn and Zn.

3.?In experiment 1, the control diet, supplemental organic Cu, Fe alone and combined diets, were randomly fed to 4 groups of one-day-old Cobb broilers (each group had 6 replicates of 4 birds).

4.?In experiment 2, the control diet, supplemental organic Mn and Zn alone or combined with Cu, Fe diets and corresponding inorganic combined diet, were randomly fed to 6 groups (each group had 8 replicates of 6 birds).

5.?In experiment 3, the depletion of organic Zn, the depletion of inorganic Zn and normal Zn treatments were carried out in three groups of one-day-old Cobb broilers (each group had 8 replicates of 6 birds).

6.?Adding organic Cu, Fe and Mn alone or combined to Zn deficient diets did not significantly improve bird performance and were mostly excreted. Supplemental organic Zn alone or combined with other elements significantly increased feed intake, body weight gain and tibia bone length. However, supplemental organic Fe alone or combined with Cu significantly increased feed intake but had no obvious effect on body weight gain. The organic Fe supplementation resulted in a wider tibia.

7.?Depletion of organic and inorganic Zn resulted in decreased feed intake, body weight gain and total tibia bone Zn content. Zinc deficiency did not affect the uptake of organic Fe by tibia bone but reduced its total Fe content.

8.?Zinc is the first limiting element among these 4 trace minerals. Adding Mn, Cu and Fe to Zn deficient diets did not stimulate bird performance. Surplus organic Fe and Cu resulted in increased feed intake and increased tibia bone Fe content but did not contribute to bird performance.  相似文献   


14.
1.?An experiment was conducted to determine the effects of supposedly unpleasant physical treatment on broiler performance, small intestinal development and ameliorating role of probiotics.

2.?The following treatments were applied from day one: (1) chicks exposed to normal human contact fed basal diet (control); (2) chicks were exposed to unpleasant physical treatment and fed basal diet (UPT-BD); and (3) chicks were exposed to unpleasant physical treatment and fed basal diet supplemented with Lactobacillus (UPT-BDL). Chicks were exposed to UPT from days 1 to 21. Different segments of gastrointestinal tract were sampled at 14, 28, 35 and 42 d of age.

3.?Broilers of UPT-BD had lower feed consumption compared with control group at 7 d of age. Overall, UPT-BDL birds showed higher body weight gain (BWG) and better feed conversion ratio (FCR) over the course of the experiment.

4.?Birds of UPT-BD had lower concentrations of lactic, propionic and butyric acids in the caecum as compared with other groups at 14 d of age. Acetic acid concentration was profoundly decreased in both UPT groups compared to the control.

5.?Duodenal villus height of UPT-BD broilers showed a slight reduction compared to the control and UPT-BDL birds at 14 d of age. Afterwards until day 42, UPT-BDL birds showed the highest villus height among treatments in different parts of the small intestine.

6.?The results suggested that, even though UPT did not have significant inhibitory effects on the development of the small intestine and broiler performance, it negatively affected bacterial metabolic end products in the caecum, which could be ameliorated by the addition of Lactobacillus.  相似文献   


15.
1.?The aim in this study was to evaluate cleaning and disinfection programmes in battery cage and on-floor layer houses in France.

2.?Cleaning and disinfection efficiency was assessed by a visual evaluation of cleaning and a bacteriological monitoring of surface contamination from counts of thermotolerant streptococci on contact agar plates.

3.?In battery cage houses, dropping belts, manure conveyors, and house floors remained highly contaminated due to poor cleaning in half of the buildings examined.

4.?In on-floor houses, a high standard of cleaning was achieved but errors in the planning of cleaning and disinfection operations sometimes led to a high residual contamination of nest boxes and egg sorting tables.  相似文献   


16.
1. The incidence of salmonellae was investigated in two batches of turkeys in the environmentally‐controlled houses and naturally ventilated pole‐barns of a commercial turkey enterprise.

2. The poults were free from contamination on arrival at the rearing house but were contaminated via the water supply from troughs which had been ineffectively fumigated.

3. Foodstuffs did not appear to be a source of contamination.

4. The spread of salmonellae in this turkey rearing enterprise was very similar to that in chicken rearing houses.  相似文献   


17.
1. The aim of the present study was to estimate gas concentrations in commercial turkey houses by Fourier Transform Infrared Spectroscopy (FTIR).

2. The experiment was conducted in 5 buildings of a commercial turkey farm. The measurements of gases were carried out every 3 weeks of the growth cycle.

3. The results demonstrate that ammonia and carbon dioxide are the prevalent gases released during the entire production cycle in turkey houses. The mean concentrations of the above compounds ranged between 4–31 ppm and 220–2058 ppm, respectively. Thiols, nitriles, amines, aldehydes, hydrocarbons and other organic and inorganic compounds also occurred in turkey houses, but they were emitted periodically and their mean concentrations were significantly lower in comparison with CO2 and NH3.

4. Lower ventilation ratio and higher moisture of excreta in the first half of the growth period accelerated the release of some gases, whereas gradual faeces and urine accumulation contributed to an increase in the concentration of selected organic compounds.

5. A portable FTIR analyser is a useful device for measuring gas concentrations in commercial turkey farms, and it supports determinations of tolerable emission limits in turkey production.  相似文献   


18.
1. The efficiency of the AvGard? (or Assur‐Rince? in the USA) trisodium phosphate poultry carcase decontamination process was evaluated during both manual and industrial trials against total aerobic mesophilic count (TAMC), thermotolerant coliforms, Pseudomonas, Enterobacteriaceae, Campylobacter, Listeria monocytogenes and Salmonella.

2. The TSP treatment proved to have significant effects on the bacterial decontamination of poultry neck skin, lowering the contamination by a factor of about 10 for TAMC and of 100 for Coliform and Pseudomonas.

3. Numeration of Salmonella with an innovative miniaturised most probable number method has proved that the effect upon these micro‐organisms was also close to 2 log10 reduction.

4. The effect of TSP treatment on the ecological balance of psychrotrophic bacterial flora was also investigated to study the origin of the shelf‐life flora of treated carcases (Pseudomonas being reduced to the limit of detection) and to ascertain whether L. monocytogenes might gain a competitive advantage. In fact AvGard? reduced the number of L. monocytogenes on poultry carcases.

5. As a consequence of the virtual elimination of the Pseudomonas usually present, Brochothrix thermosphacta became the main species responsible for putrefaction.

6. Because the growth rate of Brochothrix thermosphacta was greater than that of L. monocytogenes at refrigeration temperature, it was considered that putrefaction would occur before the emergence of large numbers of L. monocytogenes.  相似文献   


19.
1.?A factorial (2 × 3) feeding trial was set up to investigate the effects of coarse or finely ground brewers’ spent grain (BSG) and xylanase treatment, either with no xylanase, top-dressed with xylanase or pre-treated with xylanase.

2.?The experimental diets shared the same basal formulation and were fed to male broiler chickens (Ross 308) housed in individual cages from 12 to 29 d of age.

3.?Xylanase pre-treatment reduced the dietary concentration of arabinoxylan by 15–30%. Pellet durability increased when BSG was ground.

4.?Feed utilisation was significantly higher (6%) when the birds were given coarse BSG rather than ground BSG, whereas there was no significant effect of enzyme treatment. Apparent metabolisable energy was unaffected by the dietary treatments.

5.?The overall starch digestibility was high (99%), with no dietary differences, whereas ileal protein digestibility was low (57%). Xylanase top-dressing tended to improve ileal protein digestibility but, in general, xylanase treatment had no major effect on overall performance in male broilers given diets with BSG.  相似文献   


20.
1.?The effect of drinking water supplementation with lycopene on the semen quality, fertility and immunity of broiler breeders was evaluated.

2.?Broiler breeder males were individually caged from 25 to 42 weeks old and divided into two group: L group, treated birds (lycopene 0.5 g/l) and C group, control birds. Laying hens were divided into two groups and artificially inseminated.

3.?Semen variables were evaluated and daily fertility recorded. Serum bactericidal activity was tested.

4.?Semen production and viability were affected by lycopene supplementation. Serum bactericidal activity was better in L than in C group. The fertility rate curve of the L group displayed a positive trend.  相似文献   


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