共查询到20条相似文献，搜索用时 93 毫秒
1.
In this study, we investigated the optimal concentration of nutrient-mist and NaHSO3 for cutting propagation of Juniperus squamata 'Blue Star' , as well as the variation in the contents of chlorophyll in leaves, and solubility carbohydrate,soluble protein and other nutrition in leaves and bases of cuttings during rooting. Cuttings of grade Ⅲ (the lenghth of cutting bases lignified from 0.1 to 0.2 cm) was treated by 1/4MS+NaHSO3(200 mg·L-1),and the rooting rate rose 35% and root qualities were improved compared with control experiment, for there were different effects among three grade cuttings. The treatment of grade Ⅲ cuttings also resulted in higher content of soluble sugar and starch of those bases during rooting, especially obviously different for fifteen days, but less effect on the chlorophyll and some nutrition content of cutting leaves. 相似文献
2.
Effects of substrates, type of auxin, treatment concentration and time on rooting ability of Acacia mangium were systematically studied, and the change trend of rooting ability over time were analyzed. The results obtained from the experiment indicated that the substrate of yellow subsoil could improve the rooting rate of the cuttings significantly. IBA was superior to other auxins in increase the number of mean roots and the longest root length. And general rooting effect for different treatments was evaluated based on subordinate function values analysis. The optimized combinations were the cutting treated by IBA 400 mg·L-1 soaked for 2 hours. Callus were occurred in 5 d after cutting, and higher rooting period occurred in 10—15 d and 25—30 d after cutting. 相似文献
3.
The rapid propagation technology of Dendrocalamus minor var. amoenus was studied by investigating the effects of some factors such as selection of explant, phytohormone, culture method etc. The results show that: The best month for explant collection of D. minor var. amoenus is May and June. The best position for explant collection is middle-upper part knot of semi-lignification branch. The clump shoot could be induced in medium with 3/4MS+BA 4 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1. The optimal medium for subculture of D. minor var. amoenus is 3/4MS+BA 2 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1. Liquid medium is beneficial for improving growth condition and proliferation rate of clump shoot. The medium 1/5MS+IBA 8 mg·L-1+ NAA 4.5 mg·L-1 + KT 0.1mg·L-1 is a relative suitable rooting medium for D. minor var. amoenus, with the rooting method of synchronized treatment first and then rooting. The survival rate of seedlings could be higher than 90% in substrate of fine river sand∶peat soil=3∶ 1. 相似文献
4.
Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus. The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1. Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found. The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5, infection time was 20 min, culture time was 3 days. Adding 100 μmol·L-1 AS, the frequency of transient expression of GUS gene was the highest, which reached about 85% in present study. 相似文献
5.
Through Ulmus pumila cv. 'Jinye’ container-growing seedling cultivation experiment with 4 types of media or 9 media formulas, the seedling height, the stem and crown diameter indexes of U. pumila cv. jinye were measured. These indexes were significantly or extreme significantly different among various media. The growth of seedling root system varied with different media. The rooting and root number in soft media were much better than that in dense media. According to the growth traits of above-ground and underground parts of U. pumila cv. jinye seedlings, the medium suitable for U. pumila cv. jinye seedling growth was defined as media type I, whose ratio of C/N was 17.7—18.1, the density was higher than 0.26 g·cm-3. Grown on this medium, the height, stem diameter and crown diameter of U. pumila cv. jiny were 1.34—1.57 m, 1.36—1.50 cm and 59.6—73.9 cm, respectively. The results of experiment showed also that the physical property played an active role in cultural media, so a good initial medium could provide great assistance of fertilization and water management in seedling culture. 相似文献
6.
The growth dynamics of root system and aboveground of the grafted seedlings of different Camellia oleifera cultivars are studied by skeleton method and root scanning. The results of 8-months’ observation on grafted seedlings of 5 C. oleifera cultivars showed that: the growth of aerial parts and root of the C. oleifera seedlings were not entirely in sync, during the time of low temperature from November to February, the root system still in sustained growth though the aerial parts of the 5 cultivars grew slowly. Remarkable differences were existed in root length, root surface area, root volume, root biomass and other indicators after 11-months’ growth. The mean value of total length of root-system was up to 243.3 cm and 143.4 cm for the minimum, the mean value of total root surface area was up to 50.2 cm2 and 31.7 cm2 for the minimum, the mean value of root volume of the C. oleifera seedlings was up to 0.843 cm3 and 0.578 cm3 for the minimum which lived 11 months after grafting. All the root length, root surface area, root volume of the 5 cultivars have remarkable differences. 相似文献
7.
The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001.The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators. 相似文献
8.
Bi-huạ Chen 《中国林学(英文版)》2009,11(3):174-178
In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg·L–1) and NAA (0.1 mg·L–1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg·L–1) and NAA (0.1 mg·L–1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg·L–1) and acti-vated charcoal (0.5 mg·L–1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproduci-ble procedure can be adopted for large-scale propagation of T. wilfordii. 相似文献
9.
Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mechnisms of the adventitious rooting and the regulation of rooting processes are the principles and techniques of plant propagation and improvement. Microstructures and fluctuations of phytohormones in the adventitious rooting were studied with the etiolated softwood shoots of Paeonia suffruticosa ‘Yinfen Jinlin’. There are no pre-primordia in the shoots of the cultivar. Adventitious roots are produced in five stages: shoot selection, primordium initiation, primordium growth, conducting tissue differentiation and root protrusion. Primordia initiated in the cortex. The contents of the endogenous hormones, IAA, ABA and GA, were 5.842, 0.873 and 1.043 nmol•g–1 FW on the bases of shoots, respectively. CTKs which included isopentenyl adenine (iPA), zeatin riboside (ZR) and dihydrozeatin riboside (DHZR) were 0.949, 0.695 and 2.034 nmol•g–1 FW, respectively. DHZR is active among CTKs. The ratio of IAA to GA, CTK and ABA clearly increased at the stage of primordium initiation, while they showed low levels at the stages of primordium growth. The ratios were restored at the shoot levels at the stage of root protrusion. IBA provoked primordia initiation in the cortex, the vascular cambium, the pith and even in the callus induced on the base of shoots. IAA levels in the treated shoots increased gradually to its highest level (three times of control) at the stage of conducting tissue differentiation. The ratios of IAA to GA, CTK and ABA clearly decreased at the stage of primordium initiation. The ratio of IAA to ABA is regulated at 10:1. 相似文献
10.
In vitro conditions of the culture media, plant growth regulators and culture containers may cause anatomical and physiological changes that have negative effects on rooting and ex vitro acclimatization of somatic plantlets. The control of these factors could contribute to the improvement of somatic embryogenesis systems in conifers, especially in pines. The influence of macronutrient concentrations, explant type and culture containers in Pinus radiata D. Don in vitro somatic embryo rooting were analyzed. The highest rooting percentage was observed using half-strength macronutrient concentrations, complete micronutrients and vitamins of Quoirin and Lepoivre medium. Although the use of glass culture vessels was the best to increase the efficiency of the somatic embryogenesis process in terms of rooting, the use of ventilated containers resulted in a significant increase in the percentage of plants able to be planted in the field. 相似文献
11.
12.
13.
一种简便的牡丹、芍药DNA的提取方法 总被引：5，自引：0，他引：5
对近几年发展起来的植物基因组DNA的提取方法进行改良,提出一种简便实用的牡丹、芍药的DNA提取方法。得DNA的产量和纯度满足AFLP分析。 相似文献
14.
FAN Bing-you GAO Shui-pingCollege of Forestry Henan University of Science Technology HOU Xiao-gai SHI Guo-an KONG Xiang-sheng 《中国林学(英文版)》2012,14(4):291-298
The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by ACC oxidase (ACO). In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO (FJ855434) has 1281 bases, containing four exons and three introns, encoding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm. 相似文献
15.
为研究不同牡丹品种引种的适应性,对初步筛选出的8个名优牡丹品种的生长特性和开花性状进行了研究。结果表明:筛选出的8个牡丹引种品种适生性良好,能够正常地分蘖,各引进品种的花型和花色等表型表现基本与原产地相当,均能维持较高的观赏价值。通过进一步对引种适应性进行综合评价,筛选出的8个牡丹引种品种的各项得分均在10分以上,且综合评分均较高(≥70分),说明引进品种的适应性均较好,开花品质均较高,可从中筛选出引种适应性综合评分较高的品种进行进一步的引种驯化研究。 相似文献
16.
以牡丹为材料,研究了不同外植体、激素对愈伤组织诱导和继代增殖的影响,结果表明:①牡丹子叶、胚轴是诱导愈伤组织的最佳外植体;②牡丹子叶愈伤组织诱导的最佳培养基为:MS+Ca2+;③牡丹愈伤组织增殖的最佳培养基为:MS+2,4-D 0.5 mg.L-1+NAA 1.5 mg.L-1+Vc 500 mg.L-1;④在培养基中添加500 mg.L-1Vc或去除Cu2+,具有较好的抗褐化效果。 相似文献
17.
对NCBI(美国国立生物技术信息中心)中牡丹的ESTs(expressed sequence tags)的序列进行分析,结果表明:在所分析的2 204条序列中,仅324条分布有SSRs(simple sequence repeats),占全部ESTs序列的14.70%;SSR的出现频率为15.20%,共计335个,其中,二核苷酸重复比例84.18%,三核苷酸重复比例为15.22%,四和六核苷酸重复比例为0.30%。在此基础上,利用软件(serafer 1.3)设计了51对备选SSR引物,以6个滇牡丹不同花色类群DNA为模板对引物进行筛选,其中,10对引物有扩增产物;用这些引物进一步在10个类群50个DNA模板进行多态性测试,结果显示:上述10对SSR引物均有多态性,且同一类群内不同模板DNA间也存在多态性。本研究结果证明:基于牡丹EST信息建立SSR标记是一种有效而可行的方法,有助于滇牡丹遗传多样性分析及基因组学方面的研究。 相似文献
18.
【目的】探讨容器育苗技术在甘肃省天水市武山县滩歌林场林业育苗中的应用价值。【方法】选取2018年8月甘肃省天水市武山县滩歌林场的紫斑牡丹种子,种子重319.16 g·cm^-3,实验于2018年8月开始。为了更好的对比目前容器育苗技术的先进性,本次研究选取了传统林木穴盘(A)、平衡根系轻基质无纺布容器(B)、新型林木穴盘(C)。三种穴盘规格分别为:45 cm×55 cm×10 cm、45 cm×55 cm×8 cm、45 cm×55 cm×11 cm、21.5 cm×35.5 cm×11 cm。基质为珍珠岩、进口草炭。【结果】不同穴盘容器对紫斑牡丹种子生根的影响来看,B容器、C穴盘的生根时间、生根率、根长>5 cm比例、种子腐烂率均优于A穴盘的生根时间、生根率、根长>5 cm比例、种子腐烂率,而C穴盘的相关指标显著优于其它两种育苗容器,组间比较存在显著差异(P<0.05),统计学有意义。30天低温处理时:A穴盘与B容器相比萌发率无明显差异(P>0.05),两种容器与C穴盘相比,存在显著差异(P<0.05),统计学有意义。40天低温处理时:A穴盘与C穴盘相比萌发率无明显差异(P>0.05),两种容器与B容器相比,存在显著差异(P<0.05),统计学有意义。50天低温处理时:A穴盘与C穴盘相比萌发率无明显差异(P>0.05),两种容器与B容器相比,存在显著差异(P<0.05),统计学有意义,而50天的B容器与40天的B容器相比萌发率无明显差异(P>0.05),故B容器40天低温处理是紫斑牡丹种子的最佳条件。【结论】通过实验得到平衡根系轻基质无纺布容器、新型林木穴盘是林业育苗中最佳的容器育苗技术,并且平衡根系轻基质无纺布容器的侧根萌发率高于新型林木穴盘技术,因此,最终明确平衡根系轻基质无纺布容器适用于在甘肃省天水市武山县滩歌林场林业育苗中的应用价值较高,值得广泛推广。 相似文献
19.
本试验以分别接种AM真菌Glomus mosseae和G.versiforme的两年生盆栽‘凤丹’幼苗为材料,以不接种为对照,研究不同AM真菌对牡丹幼苗根系构型和生长的影响。结果表明:不同AM真菌的侵染率不同,G.mosseae的侵染率高于G.versiforme;接种G.mosseae的植株干重、根冠比和根系活力显著高于对照。接种不同AM真菌的牡丹幼苗的根系参数均比对照有不同程度的增加,其中对水平延伸根系所占比例和根系总体积的影响最为显著。相关性分析表明,水分饱和亏缺与植株干重、根冠比、根系活力等呈显著负相关;根系参数与牡丹生长呈显著正相关。不同AM真菌可通过影响牡丹幼苗的根系构型而影响其生长。G.mosseae是促进牡丹生长的高效菌种。 相似文献