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1.
Control of Lymantria dispar L. by biological agents   总被引:1,自引:0,他引:1  
The experiment on control ofLymantria dispar L by using different kinds of biological measures, including nuclear polyhedrosis virus (NPV) ofLymantria dispar L., BtMP-342, sex-attractant as well as botanical insecticide, was carried out in the forest regions of Inner Mongolia in 2003. Two concentrations (2.632×106 PIB·ml−1 and 2.632×107 PIB·ml−1) ofLymantria dispar L. NPV were sprayed on the 2rd-instar-larvae ofL. dispar and 70% and 77.8% control effect were obtained respectively. BtMP0-342 was applied to the 3rd- and 4th-instar larvae and the control effect was around 80%. The sex-attractant provided by Canada Pacific Forestry Research Center also showed a good result in trappingL. dispar adults. The self-produced botanical insecticide, which was extracted from a kind of poisonous plant distributed in Daxing'an Mountains, China, exhibited a good control result in controlling the larvae ofL. dispar, and 82% mortality was observed when spraying primary liquid of the botanical insecticide on the 3rd–5th-instar-larvae in lab. Foundation item: This paper was supported by Natural Science Foundation of Heilongjiang Province (C0235) and Youth Foundation of Harbin City (0061218024). Biography: ZHANG Guo-cai (1964), male, Ph. Doctor Candidate, associate professor in School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Song Funan  相似文献   

2.
银杏大蚕蛾的生物学和防治技术研究   总被引:6,自引:0,他引:6       下载免费PDF全文
银杏大蚕蛾在湖北一年发生一代。幼虫5龄;4、5龄幼虫有绿色型和黑色型。幼虫取食38种植物,天敌有53种。本文还叙述了该虫发生与环境的关系。防治试验表明:喷洒2.5%溴氰菊酯5000倍液、2×10~8孢子/ml白僵菌液、25%苏脲一号500倍液、1×10~8PIB/mlDjNPV虫尸液效果均很好。  相似文献   

3.
Mortality ofHeliothis armigera (Hbn.) larvae due to nuclear polyhedrosis virus (NPV) was significantly higher onGossypium barbadense (cv. DCH 32) than onG. birsutum (cv. LRA 5166) andG. arboreum (cv. K 10). A positive correlation existed between leaf area consumed and NPV mortality of the larvae. Among the three dosages of NPV tested, 2.16×108 polyhedral occlusion bodies (POB)/ml as ultra low volume application caused the maximum larval mortality. Probit analysis of time mortality responses showed that the LT50 was lower onG. barbadense than on other two species tested.  相似文献   

4.
A strain of Cytoplasmic polyhedrosis virus (CPV) was separated from the infected larva during the research of integrated pest management ofDendrolimus superans. The morphology, bioassay, histopathology and field-test for this CPV were studied. The size of CPV is 0.16 μm×1.57 μm and the virion is 16.0 nm×58.1 nm. The LC50 to the 3rd and 5th instar larva ofDendrolimus superans were 2.81×104 PIB/mL and 7.17×104 PIB/mL respectively. The polyhedrosis were formed after midgut of larva were infected for 72 h. A large amount of polyhedrosis was formed after 144 h. The mortality was more than 82% and average mortality was 84.62% when using 1.17×108 PIB/mL virus suspension to control the pest in field test. The project was supported by the Natural Sciences Foundation of Heilongjiang Province. (Responsible Editor: Chai Ruihai)  相似文献   

5.
1993年在大邑县共收集到舞毒蛾天敌30种,其中寄生性昆虫19种,捕食性昆虫9种,病毒和虫霉菌各1种。卵期平均寄生率为27.06%±0.54%,大蛾卵跳小蜂是主要天敌。幼虫期天敌有病毒、斑痣悬茧蜂、毒蛾镶颚姬蜂和绒茧蜂等,平均杀死幼虫51.59%±6.48%。蛹期主要天敌有寄蝇、广大腿小蜂和脊腿囊爪姬蜂等,平均寄生率达32.91%±26.40%。在舞毒蛾卵-蛹期,累计约有87.62%的个体被天敌致死。  相似文献   

6.
从黑龙江省双城林场采集健康的分月扇舟蛾2龄幼虫,将室内保存一年的分月扇舟蛾虫尸研碎,分离提纯得到纯净的颗粒体病毒。将病毒悬液稀释为1..58×103PIB·mL-1,1.58×104PIB·mL-1,1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-17种浓度,采集新鲜的杨树叶分别浸入上述不同浓度的悬液,对采集的健康幼虫进行接毒。9天实验结果表明,浓度为1..58×103PIB·mL-1时校正死亡率最小(7.32%),浓度是1.58×109PIB·mL-1时校正死亡率最大(97.36%),浓度对数和死亡几率值的回归直线方程为y=1.946 0.558x,LC50为2.97×105PIB·mL-1。1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-1w所对应的半致死时间(LT50)分别为8.55d,6.89d,5.9d,4.65d,4.08d。随着浓度的增大,LT50逐渐缩短,说明该病毒的毒力较强,作为杀虫剂具有很好的应用潜力。  相似文献   

7.
Zethenia rufescentaria Motsch. has outbroken successively in the larch plantation of Jixi region and seriously damaged stands since 1990. Chemical control was studied for controlling the occurrence of the pest. The results showed as follows: When the third or the forth instar larvae was controlled with smoke agent of 5% Lindane. the most suitable time was July 10–13. The area controlled was 2000 hm2. The dosage was 7.5 kg/hm2 and the mortality of the pest reached 93.5%. 25% dimilin III (flowable formulation) was used to prevent the third instar larvae. The best time was about July 5. The dosage was 150g/hm2. The mortality of the pest reached 89.85%. 3% Hexyclan dusting was also used to control adults of the pest. The best time was before the peak of their emergence (June 3). the dosage was 11.3 kg/hm2. The mortality of the pests reached 89.6%.  相似文献   

8.
The diatomaceous earth (DE) Dryacide® was tested under laboratory conditions on surfaces (2 g DE/m2) and in wheat grain (3 kg DE/t wheat) at a relative humidity of 70±5% and a temperature of 22±1°C. The wheat had a moisture content of 14.5%. The effect of surface treatments was tested onEphestia kuehniella, Oryzaephilus surinamensis, Tenebrio molitor andTribolium castaneum. The efficacy of grain treatments was determined usingEphestia elutella, O. surinamensis, Sitophilus granarius andTribolium confusum. In both treatments adult and larval stages were included.100% mortality were reached with the surface treatments in adults and larvae ofO. surinamensis and the adults ofT. molitor andE. kuehniella after an exposition time of 3–9 days. AgainstT. castaneum and the larvae ofT. molitor andE. kuehniella no complete control could be achieved.In the grain treatments the dosage of 3 kg DE/t wheat reached 100% mortality in all tested pests exceptS. granarius. Most sensitive wereO. surinamensis andE. elutella. InT. confusum the adults died within 13 days of treatment, but for complete control of the larvae 13 weeks were necessary. AgainstS. granarius the silica dust showed no satisfactory efficacy, because 49 days after beginning of the examinations beetles of the progeny hatched. Dryacide® could not prevent the reproduction of the granary weevil, but there was a lower number of progeny compared to the untreated control.  相似文献   

9.
On biological control of Boarmia bistortata Goeze (Lep., Geometridae) by help of a virusThere are very few cases of virus diseases in Geometridae known hitherto. During an outbreak ofBoarmia bistortata near Weiden (northern Bavaria) 1974, a single larva infected by a virus was found. The pathogen was reared in 16000Boarmia larvae fed with an artificial diet in 1974/75 for aid of biological control of the pest in the field. After 13 days 100% mortality of the larvae were registered. The virus was specific toBoarmia bistortata.  相似文献   

10.
Using multiple pest-mortality sources is a basic tenet of IPM, but they may not always be compatible. To that end, we examined compatibility of parasitism and nucleopolyhedrovirus (NPV) infected. Specifically, we examined the effects of prior oviposition experience by Microplitis pallidipes on its ability to distinguish NPV-infected from healthy larvae of Spodoptera litura. We found that time spent searching for hosts was significantly lower, while the number of attacks, percentage of first attacks, and parasitism rate were all significantly higher in healthy versus virus-infected hosts for parasitoids that had experienced healthy hosts. Meanwhile, parasitoids that had experienced infected hosts spent significantly more time searching for infected hosts starting on day 2. For these parasitoids, the number of attacks, percentage of first attacks, and parasitism rate were all significantly lower from day 2 in virus-infected versus healthy larvae. We found no significant differences between parasitoids without experience versus ones experienced with infected hosts in search time for infected hosts, number of infected host attacks, or percentage of first attacks on infected hosts. In comparison, parasitoids that had experienced healthy hosts spent less time searching for healthy hosts than parasitoids without experience, and displayed a higher frequency of attack on healthy hosts and a higher percentage of first attacks on healthy hosts. Experience with healthy larvae aided parasitoids in distinguishing healthy from virus-infected larvae, but experience with virus-infected larvae did not convey the same discrimination. This information provides a better understanding of how to integrate NPV applications with naturally occurring parasitism for this pest in crops.  相似文献   

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