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1.
旨在估计华南地区长白种公猪精液性状遗传参数以及分析公猪采精月龄和季节对精液性状的影响,为合理制定种公猪育种方案提供理论依据。本研究采用Asreml-R对华南地区两个公猪站1 605头长白公猪107 221条精液数据进行统计分析,利用单性状重复力动物模型估计公猪精液各性状的方差组分、遗传力和重复力,利用两性状重复力动物模型对精液体积、精液密度、精子活力和精子畸形率等性状进行遗传相关和表型相关估计;利用R语言程序中的一般线性模型分析采精月龄和季节对精液性状的影响。结果表明,精液体积和精子畸形率属于中等遗传力（0.23和0.38）,其中精子畸形率的变异系数较大,为85.42%,其余性状都为低遗传力（0.07~0.19）;精液体积与精液密度以及精子活力与精子畸形率为极显著遗传负相关（-0.77和-0.90）;精液密度与精子活力呈极显著的遗传正相关（0.50）。采精月龄对精液性状影响显著（P<0.05）,在公猪达到性成熟后,精液体积呈显著上升趋势,精液密度和精子畸形率总体呈下降趋势,精子总数和有效精子数在13~18月龄组显著高于其他组（P<0.05）。在春季精液密度最高,精子总数和有效精子数在秋、冬季显著优于春、夏季（P<0.05）。综上,长白公猪精液体积和精子畸形率具有较大的选育潜力,可作为候选性状进行选择。在公猪生产管理方面,公猪36月龄后精液产量下降,考虑更新淘汰。公猪精液品质在秋、冬季显著优于春、夏季,在中国南方夏季应提前做好降温工作。 相似文献
2.
为研究不同品种、采精月份、采精月龄和采精间隔等因素对加系公猪精液品质的影响,以及品种、初次采精周龄对精液质量稳定性的影响,本研究以江西某种公猪站79头加系大白猪、长白猪、杜洛克猪种公猪为试验群体,收集2018年12月至2020年12月3 921条精液采集与精液质量数据,通过混合线性模型与方差分析探究各因素对精液量、精液密度、精子活力、总精子数及其稳定性的影响。结果显示,从不同品种对精液质量的影响来看,长白猪精液量和总精子数均高于大白猪、杜洛克猪,但杜洛克猪精液密度高于长白猪、大白猪,杜洛克猪精子活力最低;从不同月份来看,1~3月采精精液密度最高,4~6月采精精子活力最高,10~12月采精精液量和总精子数最高,精液量呈现秋冬多、春夏少的季节变化规律。公猪不同月龄采精,精液质量指标也存在差异,月龄越小精液量越低,但精液密度偏高,精子活力相对较好,在19~24月龄黄金期总精子数最高。不同采精间隔对精液质量有较大影响,采精间隔越长,精液量、精液密度、精子活力和总精子数相对较好,采精间隔为5 d时综合性能最佳,但过长的采精间隔导致精子活力降低。品种影响总精子数稳定性,长白猪、大白猪总精子数稳定性显著优于杜洛克猪（P<0.05）。本研究结果表明,品种、采精月份、采精月龄和采精间隔均会影响公猪精液质量,关注这些因素有助于公猪站制定更完善的生产计划,提高公猪利用率。 相似文献
3.
【目的】探究品种、公猪出生胎次、公猪同窝仔猪数、公猪乳头数、采精季节、采精月龄和采精间隔对猪精液品质的影响,以及不同品种对精液质量稳定性的影响。【方法】选取909头杜洛克、长白、大白种公猪为试验群体,收集2021年4月至2022年4月27 408条精液测定记录,采用混合线性分析模型和方差分析探究品种、公猪出生胎次、公猪同窝仔猪数、公猪乳头数、采精季节、采精月龄和采精间隔对精液体积、精液密度、精子活力、直线前进运动精子比例、精子畸形率、总精子数及各精液性状稳定性的影响。【结果】从不同品种对精液品质的影响来看,长白猪精液体积和总精子数均显著高于大白猪、杜洛克猪(P＜0.05),杜洛克猪精液密度显著高于大白猪和长白猪(P＜0.05),杜洛克猪、大白猪精子活力均显著高于长白猪(P＜0.05),大白猪直线前进运动精子比例显著高于长白猪和杜洛克猪(P＜0.05),长白猪和大白猪精子畸形率均显著低于杜洛克猪(P＜0.05);从不同公猪出生胎次对精液品质的影响来看,1~3胎出生的公猪具有较高的精液品质;从不同采精季节对精液品质的影响来看,精液密度、精子活力和总精子数秋、冬季显著高于春、夏季(P＜0.05);从不同采精月龄对精液品质的影响来看,16~25月龄公猪具有较高的精液品质;从不同采精间隔对精液品质的影响来看,4~5 d为最佳采精间隔,采精间隔过长会导致精子畸形率上升;从不同公猪总乳头数来看,乳头数13~16个时,各精液性状品质都处于中等水平,有利于公猪生产应用。3个品种各精液性状间稳定性趋势不一,杜洛克猪和大白猪精液体积和精子活力的稳定性均显著高于长白猪(P＜0.05),杜洛克猪精液密度、精子畸形率和总精子数的稳定性均显著高于大白猪和长白猪(P＜0.05),大白猪直线前进运动精子比例的稳定性显著高于长白猪和杜洛克猪(P＜0.05)。各品种中,长白猪稳定性较差;各精液性状中,精子活力稳定性最好。【结论】品种、公猪出生胎次、采精季节、采精月龄和采精间隔均会影响公猪精液品质,可根据不同品种公猪制定更完善的选择方案,提高精液质量,加速公猪遗传改良。 相似文献
4.
Xiujin Li Bo Jiang Xibin Wang Xiaohong Liu Qin Zhang Yaosheng Chen 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(3):183-189
The economic profitability of a boar station largely depends on semen quantity and quality traits. However, genetic analysis of semen traits has not yet been done in the boar population in China. In this study, we aimed to estimate genetic parameters for semen traits and the influence of seasons on these traits by using data of Duroc, Landrace and Yorkshire boars in South China. The following four semen traits were analysed: semen volume (ml; VOL), sperm concentration (106/ml; DEN), sperm motility (MOT) and percentage of abnormal sperm (ABN). Genetic parameters and season effects were estimated simultaneously for each breed by using a multiple‐trait (4 × 4) repeatability animal model. The four traits had a moderate heritability with average estimates of 0.23, 0.28, 0.26 and 0.17 across the three breeds, respectively. The estimates of genetic correlations among four traits differed in the three breeds. In particular, in Yorkshire, the four traits were nearly genetically independent. The season of collecting semen had a significant impact on these four semen traits except ABN in Duroc (Bonferroni adjusted p < 0.05/6). The moderate heritabilities indicate the possibility of effective selection of boars for semen traits. Different genetic correlations for the three breeds suggest that the selection strategy for the four traits should be investigated separately for each breed. Some necessary actions should be taken to reduce the influence of seasons on semen traits. 相似文献
5.
本试验旨在研究骨桥蛋白（osteopontin,OPN）在长白公猪生殖细胞中的表达和定位,并探究其与公猪繁殖性能的相关性。试验采集了长白公猪精液和不同阶段（3日龄、3月龄、6月龄和12月龄）的睾丸组织,通过蛋白印迹的方法检测OPN蛋白在精液和不同月龄睾丸中的表达,通过免疫组化的方法对OPN蛋白在公猪睾丸细胞中进行定位;同时,根据配种胎次≥ 20胎,3次配种公猪为同一头的标准,筛选并采集17头长白种公猪精液,统计相对应的1 388头母猪的生产成绩,计算得到公猪繁殖性能指标（包括窝产总仔数、窝产活仔数、分娩率和繁殖力）。低温离心精液分离得到精子和精浆,丙酮法提取精浆蛋白,Lysis buffer方法提取精子蛋白,最后运用BCA和ELISA的方法检测精子和精浆中OPN蛋白的含量,分析OPN蛋白与公猪繁殖性能的相关性。蛋白印迹结果显示,OPN在精子、精浆和各月龄阶段的长白公猪睾丸中均以两种形式表达（67.4和33.7 ku）,且67.4 ku的形式在3月龄公猪睾丸中表达量最高;免疫组化的结果显示,OPN在长白公猪的初级精母细胞、次级精母细胞和精子细胞中表达,在精母细胞、支持细胞和间质细胞中无表达;BCA和ELISA结果显示,精子中的OPN蛋白含量是精浆中的7倍（P<0.05）,精液中的OPN蛋白与公猪窝产活仔数显著正相关（P<0.05）。本试验结果表明,OPN在各阶段的长白公猪睾丸中都有表达,且在精子和精浆中也有表达,这可能与公猪的繁殖性能有关,从而为后期OPN蛋白在公猪受精力的研究奠定基础。 相似文献
6.
本研究采用单性状重复力模型和双性状动物模型分别对公猪精液体积、精子活力、精子密度、精子畸形率4个性状进行遗传参数估计,并对不同性状间的遗传相关进行估计。结果显示:精液体积为中等遗传力(遗传力为0.29),精子密度、精子活力和精子畸形率性状具有较低遗传力,遗传力分别为0.10、0.16、0.15;这4个性状均有中等偏高的重复力,分别为0.47、0.42、0.36、0.50。精液体积与精子密度具有较强的遗传负相关(-0.389),精液体积与精子畸形率具有较弱的遗传负相关(-0.171),精子活力与精子畸形率具有强的遗传负相关(-0.826),其余性状均无明显遗传相关。以上结果表明可以通过对精液性状进行选择来提高公猪精液品质。 相似文献
7.
【目的】研究采精员、猪舍温湿度、氨气浓度和光照强度对种公猪精液品质的影响,以提高猪精液品质。【方法】选取347头(580±48) d的杜洛克公猪,收集2021年11月至2022年2月共2 966条精液测定记录,采精员做好每天公猪的采精记录,猪舍温湿度、氨气浓度和光照强度参数于每天上午09:00进行测定。使用R软件对采精员记录、环境参数和精液性状进行关联分析。【结果】(1)采精员对精液品质具有显著影响(P<0.05),8号采精员采集的精液精子活力最高、精子畸形率最低。(2)猪舍环境温度为22～25℃时,精子活力显著高于18.5～22℃(P<0.05),精子畸形率显著低于18.5～22℃(P<0.05)。(3)相对湿度在70%～77%时精子活力最高、精子畸形率最低,与相对湿度在46%～60%时差异显著(P<0.05),与相对湿度在60%～70%时无显著差异(P>0.05),相对湿度在60%～70%时精子活力、精子畸形率和总精子数都处于较高水平。(4)随着氨气浓度降低,精子活力和总精子数呈现上升趋势、精子畸形率呈下降趋势,氨气浓度2～4 ppm与5～7.3 pp... 相似文献
8.
不同品种猪精液品质的季节性变化规律研究 总被引：3，自引：0，他引：3
在不同季节采集56头大白、长白、杜洛克公猪的精液,对精液品质进行了评定和分析。结果表明:在一年内,杜洛克和大白公猪的精液品质均以秋季最好,春季次之,夏季最差,而长白公猪的精液品质以春季最好、夏季最差;精液量有显著(P<0.05)的品种间差异,长白猪、大白猪、杜洛克猪依次降低;精子活率表现为杜洛克猪、大白猪、长白猪依次降低但差异不显著(P>0.05);精子畸形率以长白猪为最高,而在各品种间差异也不显著(P>0.05)。经综合评定,在受试的3个品种公猪中,杜洛克猪的精液品质较好,而且在夏季也优于其他2个猪种。 相似文献
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本试验旨在研究不同精液品质特征长白公猪血清和精浆中元素含量的差异,分析血清和精浆中元素含量对精液品质的影响。基于107头长白公猪的1402次精液品质记录,将公猪群按照精液可利用率划分为3组:低利用率组(利用率<60%,n=21)、中等利用率组(60%≤利用率≤80%,n=27)和高利用率组(利用率>80%,n=59)。采集每头长白公猪的血清和精浆样品,采用电感耦合等离子体质谱法检测血清和精浆中营养元素和毒性元素含量。结果表明:1)低利用率组长白公猪的有效精子数和精子活力显著低于中等利用率和高利用率组(P<0.01),精子畸形率显著高于中等利用率和高利用率组(P<0.01)。2)不同利用率组间长白公猪的血清和精浆元素含量无显著差异(P>0.05);但在血清和精浆元素含量与精液品质参数相关性分析中发现,精浆铅元素含量与精子活力呈显著负相关(P<0.05),与精子畸形率呈显著正相关(P<0.05)。3)对精浆铅含量分组进一步分析发现,精浆铅含量为0μg/L时长白公猪的精子畸形率显著低于精浆铅含量>10.0μg/L时(P<0.05),精子畸形率降低约6.11%。总的来说,长白公猪精浆中毒性元素铅的存在会通过损害精子活力和形态,影响公猪精液品质。 相似文献
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【目的】 探讨负压条件下向Modena稀释剂中添加不同浓度牛磺酸对长白公猪精子常温保存质量及抗氧化能力的影响,以期为猪精液保存体系的完善提供参考。【方法】 向Modena稀释剂中分别添加浓度为0（对照组）、1、5及10 mmol/L牛磺酸,各组精液在―0.04 Mpa条件下保存11 d。于第1、3、5、7、9及11天利用计算机辅助精子分析系统（CASA）检测精子活力、活率、畸形率、平均路径速度（VAP）、曲线速度（VCL）及鞭打频率（BCF）;利用试剂盒测定精液的总抗氧化能力（T-AOC）及H2O2含量。【结果】 在保存1~11 d期间,3个牛磺酸组猪精子的活力及活率均显著高于对照组（P<0.05）;第11天时5 mmol/L牛磺酸组猪精子畸形率显著低于其他各组（P<0.05）;自第3天开始,1、5及10 mmol/L牛磺酸组猪精子的VAP均显著高于对照组（P<0.05）,其中以5 mmol/L牛磺酸组效果最佳;第11天时,5 mmol/L牛磺酸组猪精子的VCL、BCF均显著优于其他各组（P<0.05）;牛磺酸可显著提升猪精子的T-AOC （P<0.05）,但第11天时T-AOC较弱;第1~3天时,所有试验组间猪精子H2O2含量无显著差异,第5~11天时,3个牛磺酸组的H2O2含量均显著低于对照组（P<0.05）。【结论】 在―0.04 Mpa条件下,向Modena稀释剂中添加牛磺酸可以显著改善常温保存猪精液的质量参数与抗氧化性能,其中以5 mmol/L添加量最好,保存时间在9 d以内为宜。 相似文献
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Phenotypic Correlations of Testes Size with Semen Traits and the Productive Traits of Young Boars 
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下载免费PDF全文 The aim of this study was to investigate whether there is a relationship of young boar testes size with semen traits and with productive traits. The dimensions (length, width and volume) of each testis and semen traits (semen volume, percentage of sperm with progressive motility, sperm concentration, total number of sperm in semen, percentage of sperm with normal acrosome, percentage of sperm with major and minor morphological defects, osmotic resistance test value and activity of aspartate aminotransferase in seminal plasma) were determined on 120 young boars aged 6 months. At 180 day of age, the boars backfat thickness and leanness (by ultrasonic apparatus) and body weight were also measured. The average daily gain was determined in the period from 70 to 180 days of age of the boars. The results showed that the sperm concentration, total number of sperm in semen and percentage of progressive motile sperm were a significantly positively correlated with width and volume of the left (p ≤ 0.01) and right testis (p ≤ 0.05) and with total volume of both testes (p ≤ 0.01). But the highest values of correlation coefficients were found between the width of the left testis and sperm concentration, total number of sperm in semen and percentage of progressive motile sperm. A correlations of dimensions (length and width) and volume of testes with other semen traits were very low and statistically non‐significant. The volume of testes (left and right testis and total testes) was significantly positively correlated with body weight at 180 days of age and daily gain (p ≤ 0.01), but lower correlation coefficient was between left testis and daily gain (p ≤ 0.05), whereas correlations were low and non‐significant with leanness and backfat thickness. 相似文献
13.
Krzysztof Górski Stanisław Kondracki Maria Iwanina Władysław Kordan Leyland Fraser 《Animal Science Journal》2021,92(1):e13629
The aim of the study was to determine the relation between the semen quality, frequency of sperm defects, sperm dimensions and shape, and the ejaculate volume of Large White and Landrace boars. A total of 648 ejaculates collected from 31 Large White and 30 Landrace boars were divided into three groups according to the criterion of the ejaculate volume. In this study Landrace boars produced ejaculates with higher volume, sperm concentration, and total numbers of spermatozoa than Large White boars. Landrace boars also showed a lower frequency of sperm with morphological abnormalities (P < 0.05). Landrace boars sperm had larger heads, which were by 0.15 μm longer, and by a larger perimeter and area (P < 0.05). Landrace boar spermatozoa also had a longer flagellum and were generally larger and by 2.07 μm longer than Large White boar sperm (P < 0.05). Significant differences were also found in the shape of sperm of the two breeds (P < 0.05). Landrace boars sperm had more elongated heads, and the ratio of head size to flagellum length was lower than in Large White boars sperm (P < 0.05). Sperm from ejaculates with low volume had a shorter flagellum and a greater head length/flagellum length ratio than sperm from medium- and high-volume ejaculates (P < 0.05). 相似文献
14.
Genetic Parameters for Semen Traits in AI Boars Estimated from Data on Individual Ejaculates 总被引：1，自引：0，他引：1
J Wolf 《Reproduction in domestic animals》2009,44(2):338-344
Semen volume, sperm concentration, percentage progressive motility, percentage of abnormal spermatozoa and total number of sperms from 215 830 ejaculates were analysed. The ejaculates were collected between 2000 and 2005 and originated from 3675 boars of different breeds and crossbred combinations from 23 AI centres of the Czech Republic. Genetic parameters were estimated using multiple- and single-trait animal models. Factors included in the models were: breed or crossbred combination, year–month, age class of the boar, interval between subsequent semen collections, joint effect of AI centre and year, permanent environment and additive genetic effect of the boar. The estimated heritabilities for semen volume and sperm concentration were approximately 0.20, whereas the estimates were somewhat lower for motility, percentage of abnormal sperm and total number of sperms. High negative genetic correlations were estimated between semen volume and sperm concentration (−0.68 for dam breeds, −0.69 for sire breeds) and between motility and percentage of abnormal sperm (−0.93 for dam breeds and −0.59 for sire breeds). The correlations between both semen volume or sperm concentration and motility or percentage of abnormal sperm were mostly small and negligible. Repeatabilities of 0.43–0.46, 0.37–0.38, 0.29–0.35, 0.42–0.50 and 0.29–0.30 were estimated for semen volume, sperm concentration, motility, percentage of abnormal sperm and the total number of sperms, respectively. On the basis of the estimated genetic parameters presented here, effective selection on sperm characteristics, especially for volume and concentration, should be possible using an animal model. 相似文献
15.
【目的】 分析胎次、配种季节和与配公猪3个固定效应对大白母猪妊娠天数的影响,估计妊娠天数的遗传参数并探索其与总产仔数、产活仔数、初生窝重、死胎数、木乃伊数的关系。【方法】 收集2 138头大白母猪妊娠天数、总产仔数、产活仔数、初生窝重、死胎数及木乃伊数6种生产表型,共包含4个胎次（1~4）的4 261条记录。利用一般线性模型分析试验猪群的固定效应对妊娠天数的影响,并采用多性状重复力模型估计妊娠天数与其他繁殖性状的遗传参数（遗传力、重复力、遗传相关、表型相关）。【结果】 大白母猪平均妊娠天数为116.08 d,经t检验分析与114 d差异显著（P<0.05）。胎次对妊娠天数有显著影响,且第1胎妊娠期显著短于第2胎（P<0.05）;配种季节对妊娠天数有极显著影响（P<0.01）,春季配种的母猪妊娠天数最短,秋季配种的母猪妊娠天数最长,相差0.73 d （P<0.05）;与不同公猪个体配种后母猪的妊娠天数之间有显著差异（P<0.05）,差异最大达3.05 d。妊娠天数遗传力为0.1603,与总产仔数、产活仔数、初生窝重、死胎数及木乃伊数之间均呈现负表型和负遗传相关。【结论】 妊娠天数受到多种因素的影响,且具有遗传性,与母猪总产仔数等繁殖性状有显著相关,可作为养猪生产的间接指标,对准确把握母猪预产期、实现精准饲养管理具有重要意义。 相似文献
16.
17.
季节变化对不同品种种公牛精液品质及产能的影响 总被引：1，自引：0，他引：1
选择年龄在3~4.5岁的不同品种的种公牛20头,观察四季变化对其精液次均采集量、平均密度、平均活力、精液利用率及制冻数量的影响。结果显示,在四个季节中,鲁西牛春、夏季的精液密度极显著高于其他季节(P＜0.01),秋季精子活力极显著低于春季(P＜0.01),夏、秋季的精液利用率及制冻数量显著低于春季(P<0.05);蒙贝利亚牛在夏季的精液采集量显著高于其他季节(P＜0.05),夏、秋季精液密度均显著低于春季(P＜0.05,P＜0.01),夏季精子活力显著低于春季(P＜0.05),夏、秋两季精液利用率及制动数量显著低于春季(P＜0.05);德系西门塔尔牛夏季精液采集量有明显上升,显著高于冬季(P＜0.05),秋季的精液密度显著低于春季(P＜0.05),而夏、秋季精子活力显著低于春、冬季(P＜0.01,P＜0.05),春季的精液利用率及制冻数量显著高于冬季与夏、秋季(P＜0.05,P＜0.01)。综上所述,季节变化均会对各品种公牛精液指标产生不同程度的影响,其中对鲁西牛的影响较小。 相似文献
18.
Breed of Boar Influences the Optimal Concentration of Gamma‐oryzanol Needed for Semen Cryopreservation 下载免费PDF全文
下载免费PDF全文 The aim of this study was to investigate the influence of boar breed on the optimal concentration of gamma‐oryzanol on the qualities of cryopreserved boar semen. Semen was collected from 20 boars (10 Duroc, 5 Large white and 5 Landrace boars). The semen sample was divided into five groups (A–E) according to the concentration of gamma‐oryzanol in extender II, that is 0, 0.08, 0.16, 0.24 and 0.32 mm , respectively. The semen was cryopreserved by nitrogen vapour and storage in nitrogen tank (?196°C). After storage for a week, samples were thawed at 50°C for 12 s and evaluated for progressive motility, sperm viability and acrosome integrity. The results demonstrated that gamma‐oryzanol significantly improved progressive motility, viability and acrosome integrity of frozen–thawed boar semen. Considering the influence of breeds on the optimal concentration of gamma‐oryzanol, for Duroc boar, gamma‐oryzanol at 0.16 mm (group C) yielded the highest percentage of progressive motility, sperm viability and acrosome integrity. For Large white and Landrace boars, gamma‐oryzanol at 0.24 mm (group D) showed a significantly higher percentage of progressive motility, viability (not significant in Landrace) and acrosome integrity than other concentrations. In conclusion, the optimal concentration of gamma‐oryzanol needed for boar semen cryopreservation in lactose–egg yolk (LEY) freezing extender is not only depended on individual boar but also breed of boar, that is 0.16 mm for Duroc and 0.24 mm for Large white and Landrace. 相似文献
19.
Quanshun Mei Chuanke Fu Goutam Sahana Yilong Chen Lilin Yin Yuanxin Miao Shuhong Zhao Tao Xiang 《Journal of animal science》2021,99(7)
Semen traits are crucial in commercial pig production since semen from boars is widely used in artificial insemination for both purebred and crossbred pig production. Revealing the genetic architecture of semen traits potentially promotes the efficiencies of improving semen traits through artificial selection. This study is aimed to identify candidate genes related to the semen traits in Duroc boars. First, we identified the genes that were significantly associated with three semen traits, including sperm motility (MO), sperm concentration (CON), and semen volume (VOL) in a Duroc boar population through a genome-wide association study (GWAS). Second, we performed a weighted gene co-expression network analysis (WGCNA). A total of 2, 3, and 20 single-nucleotide polymorphisms were found to be significantly associated with MO, CON, and VOL, respectively. Based on the haplotype block analysis, we identified one genetic region associated with MO, which explained 6.15% of the genetic trait variance. ENSSSCG00000018823 located within this region was considered as the candidate gene for regulating MO. Another genetic region explaining 1.95% of CON genetic variance was identified, and, in this region, B9D2, PAFAH1B3, TMEM145, and CIC were detected as the CON-related candidate genes. Two genetic regions that accounted for 2.23% and 2.48% of VOL genetic variance were identified, and, in these two regions, WWC2, CDKN2AIP, ING2, TRAPPC11, STOX2, and PELO were identified as VOL-related candidate genes. WGCNA analysis showed that, among these candidate genes, B9D2, TMEM145, WWC2, CDKN2AIP, TRAPPC11, and PELO were located within the most significant module eigengenes, confirming these candidate genes’ role in regulating semen traits in Duroc boars. The identification of these candidate genes can help to better understand the genetic architecture of semen traits in boars. Our findings can be applied for semen traits improvement in Duroc boars. 相似文献
20.
SHI Li-guang ZHOU Xiong XUN Wen-juan HOU Guan-yu ZHOU Han-lin HUANG Xian-zhou 《中国畜牧兽医》2016,43(1):101-107
This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress. 相似文献