共查询到20条相似文献，搜索用时 62 毫秒
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The human interleukin-2 receptor is an inducible growth factor receptor present on the surface of activated T lymphocytes. The receptor is required for a normal T-cell immune response. High-resolution fluorescence-activated chromosome sorting and DNA spot-blot analysis with complementary DNA's for the interleukin-2 receptor indicated that the receptor gene was located on chromosome 9, 10, 11, or 12. In situ hybridization studies showed that the interleukin-2 receptor gene is on the short arm of chromosome 10, p14----15. 相似文献
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Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression 总被引：72，自引：0，他引：72
B Hoyos D W Ballard E B?hnlein M Siekevitz W C Greene 《Science (New York, N.Y.)》1989,244(4903):457-460
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根据GenBank上发表的猪白细胞介素-2（Interleukin．2,IL-2）基因序列,设计1对引物。采用RT-PCR技术,以ConA刺激的猪外周血淋巴细胞为材料,从总RNA中扩增出484bp的特异性片段,将其克隆入pGEM-Teasy载体。序列测定表明;扩增片段为猪IL-2基因,该基因与GenBank上发表的猪IL-2基因的核苷酸序列同源性达99．8％。将其定向克隆至原核表达载体pET32a,构建了表达重组猪IL-2的基因工程菌株,经IPTG诱导表达和层析纯化,获得了纯化的重组猪IL-2蛋白。 相似文献
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The x gene is essential for HTLV replication 总被引：44，自引：0，他引：44
I S Chen D J Slamon J D Rosenblatt N P Shah S G Quan W Wachsman 《Science (New York, N.Y.)》1985,229(4708):54-58
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Structure of the human interleukin-2 receptor gene 总被引：38，自引：0，他引：38
W J Leonard J M Depper M Kanehisa M Kr?nke N J Peffer P B Svetlik M Sullivan W C Greene 《Science (New York, N.Y.)》1985,230(4726):633-639
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The interleukin-2 T-cell system: a new cell growth model 总被引：98，自引：0，他引：98
Synchronized interleukin-2 receptor-positive T cells, homogeneous immunoaffinity-purified interleukin-2, and a monoclonal antibody to interleukin-2 receptors were used to show that only three factors are critical for T-cell cycle progression: interleukin-2 concentration, interleukin-2 receptor density, and the duration of the interleukin-2 receptor interaction. Since the proliferative characteristics of T cells are identical to those of both prokaryotic and all other eukaryotic cells, these findings provide a new model that accounts fully for the variables that determine cell cycle progression. 相似文献
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Regulation of expression of the interleukin-2 receptor on hematopoietic cells by interleukin-3 总被引：4，自引：0，他引：4
M C Birchenall-Sparks W L Farrar D Rennick P L Kilian F W Ruscetti 《Science (New York, N.Y.)》1986,233(4762):455-458
Remarkable similarities in the intracellular and genetic events occur when lymphoid and hematopoietic cells are exposed to their specific growth factors. The interleukin-2 (IL-2) receptor, whose cell-surface expression is an absolute requirement for the growth and differentiation of lymphoid cells, was detected on various nonlymphoid hematopoietic cell types in this study. Cell lines consisting either of granulocyte-macrophage precursors or mast cells, which are dependent on interleukin-3 (IL-3) for their growth, expressed high levels of the IL-2 receptor on their surface. Analysis of the binding characteristics of these receptors with 125I-labeled recombinant IL-2 revealed that only receptors with low affinity for IL-2 were present on these cells. Addition of purified recombinant IL-3 to these cell lines led to an increase in IL-2 receptor gene expression within 1 hour in isolated nuclei. This IL-3--induced increase in the number of IL-2 receptors on the cell surface is maximal within 24 hours. Addition of 10,000 units of IL-2 to these cells had no apparent effect on their growth or differentiation. The presence of the receptor with only low affinity for IL-2 on hematopoietic cells and the regulation by IL-3 suggest that this receptor is involved in some important metabolic event in hematopoiesis. 相似文献
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T M Williams D Moolten J Burlein J Romano R Bhaerman A Godillot M Mellon F J Rauscher J A Kant 《Science (New York, N.Y.)》1991,254(5039):1791-1794
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HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene 总被引：91，自引：0，他引：91
D W Ballard E B?hnlein J W Lowenthal Y Wano B R Franza W C Greene 《Science (New York, N.Y.)》1988,241(4873):1652-1655
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The structure, function, and expression of interleukin-2 receptors on normal and malignant lymphocytes 总被引：56，自引：0，他引：56
T A Waldmann 《Science (New York, N.Y.)》1986,232(4751):727-732
Antigen or mitogen-induced activation of resting T cells induces the synthesis of interleukin-2 (IL-2) as well as the expression of specific cell surface receptors for this lymphokine. Failure of the production of either IL-2 or its receptor results in a failure of the T-cell immune response. The receptor is composed of a 33,000-dalton (251-amino acid) peptide precursor that is post-translationally glycosylated into the mature 55,000-dalton form. In contrast to resting T cells, human T-cell lymphotrophic virus I (HTLV-I)-associated adult T-cell leukemia cells constitutively express large numbers of IL-2 receptors. Because IL-2 receptors are present on the malignant T cells but not on normal resting cells, clinical trials have been initiated in which patients with adult T-cell leukemia are treated with a monoclonal antibody that binds to the IL-2 receptor. 相似文献
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A large deletion within the T-cell receptor beta-chain gene complex in New Zealand white mice 总被引：16，自引：0，他引：16
The T-cell receptor beta-chain gene complex contains a duplication of D beta, J beta, and C beta gene segments in mice and man. When DNA from many inbred strains of mice was screened an unusual allele of the beta locus was identified in New Zealand White (NZW) mice. This allele is distinguished by the deletion of an 8.8-kilobase segment of DNA containing C beta 1, D beta 2 and the J beta 2 cluster. Despite the fact that all NZW T-cell receptors must be derived from a single set of beta-chain gene segments, this strain has functional T cells and is phenotypically normal. This deletion of T-cell receptor beta-chain segments occurs in a strain known to contribute to lupus-like autoimmune disease. 相似文献
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Interleukin-2 induction of T-cell G1 progression and c-myb expression 总被引：36，自引：0，他引：36
In studies to determine the biochemical mechanisms responsible for cell proliferation, synchronized T cells were used as a model for cellular growth control. By metabolic and morphologic criteria, it was found that activation of the T-cell antigen receptor rendered the cells responsive to interleukin-2 (IL-2), but did not move them through the cell cycle. Instead, IL-2 stimulated G1 progression to S phase, or lymphocyte "blastic transformation." During IL-2-promoted G1 progression, expression of the cellular proto-oncogene c-myb was induced transiently at six to seven times basal levels, maximal levels occurring at the midpoint of G1. 相似文献
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贵州白香猪的白细胞介素2基因的克隆及其序列分析 总被引：1，自引：0，他引：1
为获得贵州白香猪白细胞介素2基因,以提取的经刀豆蛋白A诱导培养的贵州白香猪外周血淋巴细胞总RNA为模板,扩增全长猪白细胞介素2(IL-2)基因,并克隆至pMD18-T Simple载体后测序。结果表明:贵州白香猪IL-2基因ORF长为465bp,可编码154个氨基酸,其中前20个氨基酸为信号肽,后134个氨基酸为成熟肽,具有一个潜在的N-糖基化位点;贵州白香猪除与FJ543109(99.4%)、Neijiang(98.7%)、JN851821(98.7%)、AB194099(98.5%)氨基酸一致性相对较低外,与其他猪源IL-2的一致性均为100%,而与其他种属动物的IL-2基因氨基酸一致性则较低,为11.9%～75%。 相似文献
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Expression of the pX gene of HTLV-I: general splicing mechanism in the HTLV family 总被引：43，自引：0，他引：43
Human T-cell leukemia virus type I (HTLV-I) is an etiological agent of adult T-cell leukemia. A viral gene pX encodes for p40X and it has been proposed that this protein trans-activates the viral long terminal repeat and possibly some cellular genes; this activation may be associated with T-cell transformation. The mechanism of pX gene expression and the primary structure of p40X are now reported. Two-step splicing generates the 2.1-kilobase pX mRNA; the initiator methionine for env becomes part of the pX protein. These splicing signals are conserved among all members of the HTLV family except for the acquired immune deficiency syndrome-associated viruses. 相似文献
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Monocyte-derived human B-cell growth factor identified as interferon-beta 2 (BSF-2, IL-6) 总被引：36，自引：0，他引：36
G Tosato K B Seamon N D Goldman P B Sehgal L T May G C Washington K D Jones S E Pike 《Science (New York, N.Y.)》1988,239(4839):502-504
Soluble products of either Epstein-Barr virus (EBV)-infected B cells or activated monocytes promote the proliferation of EBV-infected B cells and permit their growth at low cell densities. This suggests that growth factors are important for B-cell immortalization by EBV. In this study, a monocyte-derived factor that promotes the growth of EBV-infected b cells was purified and identified as interferon-beta 2 (IFN-beta 2), which is also known as 26-kilodalton protein, B-cell differentiation factor (BSF-2), and interleukin-6 (IL-6). The purified protein has a specific activity of approximately 4 X 10(7) units per milligram of protein in assays of B-cell growth. Thus, IFN-beta 2/BSF-2 is a B-cell growth factor that promotes the proliferation of human B cells infected with EBV. 相似文献