共查询到20条相似文献，搜索用时 62 毫秒
1.
为探讨中国美利奴羊不同甘露(聚)糖结合凝集素(MBL)型个体感染绵羊肺炎支原体的免疫应答变化,对4种不同MBL型个体进行MBL水平测定,选择其中20只作为对照组,50只为试验组,在相同饲养条件下试验组人工感染绵羊肺炎支原体,分别在攻毒前1 d(-1 d)、攻毒后1 d(1 d)、1周(7 d)、2周(14 d)、3周(21 d)用ELISA方法定量分析血清中TNF-α、IFN-γ、补体C1、C3水平.结果显示,A型和B型其MBL浓度较低,C型MBL浓度较高,与对照组相比,在攻毒后1周,A型和B型IFN-γ表达显著降低(P＜o.05),攻毒后2周,补体C3表达显著降低,TNF-α升高显著(P＜0.05);与A型和B型相比,在攻毒后1周,C型IFN-γ表达增加(P＜0.05),在攻毒后2周,补体C3表达增加、TNF-α显著降低(P＜o.05),补体C1各组均不显著.结论:低血清MBL浓度与绵羊支原体肺炎有一定的相关性,不同基因型之间其TNF-α、IFN-γ、补体C1、C3水平有差异,低浓度MBL绵羊更易发生比较严重的炎症反应. 相似文献
2.
X Wang Z Ju J Huang M Hou L Zhou C Qi Y Zhang Q Gao Q Pan G Li J Zhong C Wang 《Veterinary immunology and immunopathology》2012,148(3-4):311-319
Mannose-binding lectin (MBL), a calcium-dependent collagenous lectin, plays an important role in the host immune defence against a wide range of pathogens. There are MBL1 and MBL2 genes which encode the MBL-A and MBL-C proteins, respectively. This study was carried out to investigate the relationship between the variants of the bovine MBL2 gene and milk production traits, mastitis, serum MBL-C levels and hemolytic complement activity in both classical pathway (CH50) and alternative pathway (ACH50) in Chinese Holstein cattle. Four single-nucleotide polymorphisms (SNPs) in the exon 1 of the MBL2 gene in Chinese Holstein cattle and Luxi yellow cattle were identified by the direct sequencing method. The SNP g.201 G>A was identified as a non-synonymous mutation (codon 31, Arg>Gln) at the N-terminus cysteine-rich domain and the SNPs g.234 C>A and g.235 G>A (codon 42) made Pro to Gln at the 1st Gly-X-Y repeat of the collagen-like domain, while the SNP g.244 T>C (codon 45) was identified as a synonymous mutation (Asn>Asn) at the 2th Gly-X-Y repeat of the collagen-like domain. The SNP markers (g.201 G>A, and g.234 C>A) were significantly correlated with somatic cell score (SCS) (P<0.05). The concentration of MBL-C protein in serum ranges from 0.8 to 7.4μg/mL by enzyme-linked immunosorbent assay. Six combinations of different haplotypes from the four SNPs were identified in Chinese Holstein cattle. Statistical analysis revealed that cows with the haplotype combination H4H5 exhibited the lowest SCS. The CH50 value of H4H5 and H5H5 cow are significantly higher than H2H5 haplotype combination (P<0.05). The association analysis results showed that the haplotype combination H4H5 may be used as a tolerance haplotype combination for the bovine mastitis. 相似文献
3.
根据GenBank发表的绵羊骨形态发生蛋白6（Bone morphogenetic protein6,BMP6）基因部分序列所包含的外显子5、6、7和小鼠BMP6基因外显子5、6、7序列设计3对引物,采用PCR-SSCP技术检测BMP6基因外显子5、6和7在小尾寒羊、湖羊、多赛特、特克塞尔、考力代5个绵羊品种301个个体中的单核苷酸多态性。结果发现这3对引物的扩增片段在检测的5个品种中均无多态性,说明所检测的BMP6外显子5、6、7序列比较保守。同时克隆了小尾寒羊（695bp）和济宁青山羊（699bp）部分BMP6mRNA以及小尾寒羊BMP6基因外显子3—4的内含子序列和两端部分外显子序列（785bp）。发现小尾寒羊和济宁青山羊的核苷酸和氨基酸序列差异很小,两者的核苷酸序列同源性高达99．28％,氨基酸序列同源性为98．1％,除济宁青山羊中插入一个丙氨酸外,两者只有4个氨基酸的差异。绵羊与牛、人、小鼠和大鼠的核苷酸同源性分别为97．04％、81．82％、84．68％和85．06％;氨基酸序列同源性分别为99．05％、91．43％、90．48％和92．38％,均大于90％,表明各物种BMP6核苷酸序列虽然差异较大,但氨基酸序列却非常保守。 相似文献
4.
为建立不同甘露(聚)糖结合凝集素(MBL)基因型绵羊支原体肺炎的动物疾病模型,本研究选择MBL外显子1中4种不同基因型共32只绵羊作为试验组,6只健康羊作为对照组,在人工感染绵羊肺炎支原体3周后全部迫杀,取肺脏组织做病理切片,以组织病理学评分确定肺部的炎症反应程度.不同MBL型绵羊的肺脏呈现不同程度病理改变,组织病理学评分结果为MBLA型和B型平均分为18.3和19.1,表现为重度病变;MBLD型平均分为12.3,为中度病变;MBL C型平均分为8.9,为轻度病变.本研究以组织病理学评分方法客观量化了不同MBL基因型肺部炎症反应的严重程度,根据评分结果推测MBL A型和B型为易感型,C型为抗性型.本研究利用组织病理学评分方法对其肺炎程度进行量化评价,为筛选绵羊支原体肺炎抗性基因型提供依据. 相似文献
5.
Wang C Liu M Li Q Ju Z Huang J Li J Wang H Zhong J 《Veterinary immunology and immunopathology》2011,139(2-4):229-236
Mannan-binding lectin (MBL), a pattern recognizing serum protein, participates in the innate immune system of mammals as an opsonin. In humans, single-nucleotide polymorphisms (SNPs) in MBL2 gene were found to cause various innate immune dysfunctions. In the present study, we discovered three single-nucleotide polymorphisms of the MBL1 gene in Chinese native cattle and analyzed their associations with milk traits. By screening the genetic variation of MBL1 in 1053 individuals of three Chinese native cattle breeds including China Holstein, Luxi Yellow and Bohai Black using created restriction site–polymerase chain reaction (CRS–PCR), PCR–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing techniques, three new SNPs, g.855G>A, g.2651G>A and g.2686T>C, were found to have allele frequencies of 0–12.65%, 24.07–42.39% and 56.95–73.68%, respectively. While SNP g.855G>A is located within intron ?, the other two SNPs reside in the exon II region with one mutation being non-synonymous (GTT (Val) > ATT (Ile)) and the other synonymous (GCT (Ala) > GCC (Ala)). Among the 596 Chinese Holstein cattle with at least 3 lactation Dairy Herd Improvement (DHI) records, eight different haplotypes and 19 genotype combinations were detected. Statistical analyses revealed no correlation between either g.855G>A or g.2686T>C and somatic cell score (SCS), however significant association was found between g.2651G>A and SCS, suggesting a possible role of this SNP in the host response against mastitis. Our data also suggested that the combined genotypes of GGC/AAC with the lowest SCS, AAT/AAT with the highest protein content and AGC/AGC with the highest 305-d milk yield were favorable combinations for mastitis resistance and milk production traits. Therefore, GGC/AAC, AAT/AAT and AGC/AGC can be used as possible candidates for marker-assisted selection in the dairy cattle breeding program. 相似文献
6.
7.
绵羊雌激素受体基因外显子4多态性分析 总被引：4，自引：2，他引：2
根据GenBank发表的人、鸡、大鼠雌激素受体(estrogen receptor,ESR)基因外显子4的序列设计1对引物,采用PCR SSCP技术分析ESR基因外显子4在高繁殖力绵羊品种（小尾寒羊和湖羊）和低繁殖力绵羊品种（特克塞尔、中国美利奴、考力代和杜泊）中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。结果表明,ESR基因的此对引物扩增片段在所检测的6个绵羊品种中均不存在PCR SSCP多态性,说明所检测的ESR基因外显子4序列比较保守,该区域可能不是影响绵羊高繁殖力的功能结构域。 相似文献
8.
Schou TW Permin A Christensen JP Cu HP Juul-Madsen HR 《Comparative immunology, microbiology and infectious diseases》2010,33(3):183-195
The present study is the first demonstration of an association of the genetic serum Mannan-binding lectin (MBL) concentration with bacterial infections in chickens. The genetic serum MBL concentration was determined in two chicken breeds, and the association with the specific Pasteurella multocida humoral immune response during an experimental infection was examined. Furthermore, we examined the association of the genetic serum MBL concentration with systemic infection. The chickens with systemic infection had a statistically significant lower mean serum MBL concentration than the rest of the chickens, suggesting that MBL plays an important role against P. multocida. A statistically significant negative correlation was found between the specific antibody response and the genetic serum MBL concentration for both breeds. This indicates that MBL in chickens is capable of acting as the first line of defence against P. multocida by diminishing the infection before the adaptive immune response takes over. 相似文献
9.
《Veterinary immunology and immunopathology》2015,163(3-4):125-133
The Apolipoprotein B mRNA-editing catalytic polypeptide-like 3 (APOBEC3) genes are able to inhibit the replication of a wide range of exogenous retroviruses, as well as endogenous retroviruses and retrotransposons.Three APOBEC3 genes, named APOBEC3Z1, APOBEC3Z2 and APOBEC3Z3, have been described in sheep.In this work the three genes have been screened in order to identify polymorphisms. No polymorphism was detected for the A3Z2 and A3Z3 genes but 16 SNPs and a 3-bp deletion were found in the A3Z1 gene. A thermoestability prediction analysis was applied to the detected amino acidic SNPs by three different programs. This analysis revealed a number of polymorphisms that could affect the protein stability. The SNPs of the 3′UTR were tested to detect alterations on the predicted microRNA target sites. Two new microRNA target sites were discovered for one of the alleles.Two SNPs were selected for association studies in relation with the retroviral disease Visna/Maedi in Latxa and Assaf sheep breeds. Although association analyses resulted unconclusive, probably due to the unsuitability of the SNP allele frequency distribution of the selected polymorphisms in the analyzed breeds, these genes remain good candidates for association studies. 相似文献
10.
研究采用生物信息学方法分析了猪MBL2基因,进一步获得猪MBL2基因序列特征及编码蛋白的结构和功能,并且对CDS区里的编码蛋白的理化性质进行了预测。除此之外还对这些编码蛋白是亲水还是疏水、它们的糖基化位点、信号肽、二级结构以及三级结构进行了预测。实验结果表明,猪的MBL2基因的大小是723 bp,编码的氨基酸共有240个,蛋白质的相对分子质量为25 522.83 KDa,理论等电点PI为4.97。MBL2基因的二级结构是无规则的卷曲型,蛋白结构预测结果是一种可溶性蛋白,具有4个外显子;在第20-21位氨基酸之间存在信号肽,表现为亲水性。研究结果为进一步研究猪MBL2基因的功能提供了理论基础。 相似文献
11.
O. L. Nielsen J. C. Jensenius P. H. Jrgensen S. B. Laursen 《Veterinary immunology and immunopathology》1999,70(3-4):309-316
Mannan-binding lectin (MBL) is a serum collectin which is believed to be an opsonin of the innate immune defence against various microorganisms. MBL is a minor acute phase reactant in man. We investigated the concentration of serum MBL in chickens infected with infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The concentration of serum MBL increased about twofold (from approximately 6 to 12 μg/ml) due to these viral infections. The concentration peaked 3–7 days after infection with IBV, and 3–5 days after ILTV infection, depending on the ILTV strain used. The increased levels returned to normal values 6–10 days after infection. The results indicated that MBL is a minor acute phase reactant in chickens. 相似文献
12.
试验旨在探讨心脏脂肪酸结合蛋白（heart fatty acid-binding protein，H-FABP）基因在绵羊中的遗传多态性，并寻找可用于辅助选择的分子标记。本研究以滩羊（250只）及滩羊×湖羊杂交F1代（174只）为试验动物，利用SNaPshot分型技术对H-FABP基因（GenBank登录号：AY157617）的多态位点进行单核苷酸多态性（SNP）分析，统计基因频率和基因型频率，进行Hardy-Weinberg平衡性检测，计算期望杂合度（He）、多态信息含量（PIC）和有效等位基因数（Ne）等遗传多态指标，分析候选基因不同基因型与体重、体长、体高、胸围、胸深、胸宽和管围等生长性状的关联性。结果显示：①检测到9个多态位点：939[A/G]、980[G/A]、1018[T/C]、2878[C/T]、2956[C/T]、3017[G/A]、3341[G/C]、3394[T/A]、1056[-/G]，其中有6处转换、2处颠换、1处单碱基插入。②939[A/G]、980[G/A]、2956[C/T]、3341[G/C]、3394[T/A]和1018[T/C]的He为0.3200~0.4666，PIC为0.2688~0.3577；2878[C/T]、3017[G/A]位点的He为0.0283~0.1272，PIC为0.0279~0.1191，为低度多态；1056[-/G]位点的He在滩羊、滩羊×湖羊杂交F1代群体中分别为0.0120和0，PIC在滩羊、滩羊×湖羊杂交F1代群体中分别为0.0119和0；9个多态位点在滩羊、滩羊×湖羊杂交F1代群体中均符合Hardy-Weinberg平衡定律。③5个多态位点：939[A/G]、980[G/A]、2956[C/T]、3341[G/C]、3394[T/A]处于紧密连锁（D'>0.99），将H-FABP基因分为3个单倍型：AA、AB和BB。④在41只滩羊×湖羊杂交F1代羊中，BB单倍型在各生产性状上具有最大值，但各单倍型间差异不显著（P>0.05）。结果表明，绵羊H-FABP基因具有丰富的遗传多态性，939[A/G]、980[G/A]、2956[C/T]、3341[G/C]、3394[T/A]5个位点紧密连锁，BB单倍型可能是与绵羊生产性状相关的优势单倍型。 相似文献
13.
Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black) 总被引：3，自引：0，他引：3
Nakamitsu S Miyazawa T Horiuchi M Onoe S Ohoba Y Kitagawa H Ishiguro N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):27-33
To assess relationships between nucleotide polymorphisms of the prion protein (PRNP) gene and susceptibility to bovine spongiform encephalopathy (BSE), we investigated polymorphisms in the open reading frame (ORF) and 2 upper regions of the PRNP gene from 2 Japanese cattle breeds: 863 healthy Holstein cattle, 6 BSE-affected Holstein cattle, and 186 healthy Japanese Black (JB) cattle. In the ORF, we found single-nucleotide polymorphisms (SNPs) at nucleotide positions 234 and 576 and found 5 or 6 copies of the octapeptide repeat, but we did not find any amino acid substitutions. In the upper region, we examined 2 sites of insertion/deletion (indel) polymorphisms: a 23-bp indel in the upper region of exon 1, and a 12-bp indel in the putative promoter region of intron 1. A previous report suggests that the 23-bp indel polymorphism is associated with susceptibility to BSE, but we did not find a difference in allele frequency between healthy and BSE-affected Holstein cattle. There were differences in allele frequency between healthy Holstein and JB cattle at the 23- and 12-bp indels and at the SNPs at nucleotide positions 234 and 576, but there was no difference in allele frequency of the octapeptide repeat. We identified a unique PRNP gene lacking a 288-bp segment (96 amino acids) in DNA samples stocked in our laboratory, but this deletion was not found in any of the 1049 cattle examined in the present study. The present results provide data about variations and distribution of the bovine PRNP gene. 相似文献
14.
15.
M.X. Chu X.C. Wang M. Jin R. Di H.Q. Chen G.Q. Zhu L. Fang Y.H. Ma & K. Li 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(1):63-68
A single nucleotide polymorphism of 5' flanking region of the prolactin gene was investigated in both high prolificacy breeds (Small Tail Han and Hu sheep) and low prolificacy breeds (Dorset and Suffolk sheep) using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP). The results indicated that two genotypes (AA and AB) were detected in Small Tail Han sheep (n = 239), only one genotype (AA) was detected in Hu (n = 40), Dorset (n = 50) and Suffolk sheep (n = 39). The mutant homozygous genotype (BB) was not detected in four sheep breeds. In Small Tail Han sheep (n = 239), the frequency of genotypes AA and AB was 0.91 and 0.09, the frequency of the A and B alleles was 0.95 and 0.05, respectively. The fitness tests showed that the Small Tail Han sheep population was in Hardy–Weinberg equilibrium. Sequencing revealed a mutation (G→T) at the position 63 bp of the 5' flanking region of prolactin gene in AB genotype compared with AA genotype in Small Tail Han sheep. The Small Tail Han ewes with AB genotype had 0.83 (p < 0.05) lambs more than those with AA genotype. These results preliminarily showed that the prolactin locus is either a major gene that influences the high prolificacy in Small Tail Han sheep or is in close linkage with such a gene. 相似文献
16.
试验旨在研究绵羊催乳素受体(Prolactin receptor,PRLR)基因g.38976954C>A位点多态性和可卡因-苯丙胺调控转录肽(CART prepropeptide,CARTPT)基因g.9856267C>A、g.9854141G>C位点多态性与绵羊产羔数之间的关系,为绵羊多羔新品系的选育和高繁殖力机理研究提供参考。采用重测序和Sequenom MassARRAY?誖SNP技术对常年发情(小尾寒羊、湖羊、策勒黑羊)和季节性发情(草原型藏羊、苏尼特羊、滩羊、萨福克羊)的不同绵羊品种PRLR、CARTPT基因进行检测分型,然后与小尾寒羊产羔数关联分析。结果表明:g.38976954C>A位点有CC、CA和AA三种基因型,该位点基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异不显著(P>0.05);g.9856267C>A位点存在CC、CA和AA三种基因型,g.9854141G>C位点存在GG、GC和CC三种基因型,这2个位点的基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异均极显著(P<0.01)。群体遗传学分析结果表明,g.38976954C>A位点在小尾寒羊、草原型藏羊、滩羊和策勒黑羊中均表现为低度多态(PIC<0.25),在苏尼特羊、湖羊和萨福克羊中均表现为中度多态(0.25A位点在7个绵羊品种中均表现为低度多态(PIC<0.25);g.9854141G>C位点在小尾寒羊、苏尼特羊、草原型藏羊、滩羊、湖羊和策勒黑羊中均表现为低度多态(PIC<0.25),在萨福克羊中表现为中度多态(0.25A位点在7个绵羊品种中均处于哈代-温伯格不平衡状态(P<0.05);g.9856267C>A位点在草原型藏羊和湖羊中处于哈代-温伯格不平衡状态(P<0.05);g.9854141G>C位点在7个绵羊品种中均处于哈代-温伯格平衡状态(P>0.05)。关联分析表明,PRLR基因g.38976954C>A位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),该位点不适用于小尾寒羊多羔性状选育;CARTPT基因g.9856267C>A和g.9854141G>C位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),但这2个位点不同基因型各胎产羔数相差较大,推测该位点可能对产羔有影响。 相似文献
17.
试验旨在探究CLPG(Callipyge)与MSTN(Myostatin)基因作为绵羊生长性状候选基因的可能性,调查与绵羊生长性状相关的分子遗传标记。本试验以133只澳洲白羊×杜泊羊×湖羊杂交绵羊为研究对象,利用PCR产物直接测序及PCR-RFLP技术检测CLPG与MSTN基因的单核苷酸多态性,然后通过SPSS22.0软件GLM统计模型分析多态位点不同基因型及聚合基因型与绵羊生长性状的关联性。测序结果表明,CLPG基因STS序列232bp处检测到C→T突变位点C1,MSTN基因3'UTR区检测到G→A突变位点M1。PCR-RFLP分析显示,C1位点表现为2种基因型:CC和CT;M1位点表现为2种基因型:GG和GA。关联分析表明,C1位点与绵羊背膘厚和眼肌面积显著或极显著相关(P<0.05;P<0.01),M1位点与绵羊体重、管围、背膘厚和眼肌面积显著或极显著相关(P<0.05;P<0.01)。聚合基因型对体重、背膘厚和眼肌面积影响极显著(P<0.01)。研究揭示CLPG与MSTN基因多态性及其聚合基因型对绵羊生长性状具有显著影响,C1和M1位点可以考虑作为绵羊生长性状的有效遗传标记。 相似文献
18.
HU Shi-jin ZHANG Shu-zhen YUAN Ze-hu XUAN Jun-li MA Xiao-meng ZHANG Li ZHAO Fu-ping WEI Cai-hong DU Li-xin 《中国畜牧兽医》2016,43(5):1285-1293
The study was conducted to explore the possibility that CLPG (Callipyge) and MSTN (Myostatin) genes which could be the candidate genes of sheep growth traits, and investigate the molecular genetic markers related to sheep growth traits.133 (Austrilian White sheep×Dorper sheep×Hu sheep) hybid-sheep were chosen as subjects, the technology of direct sequencing of PCR products and PCR-RFLP were used to detect the single nucleotide polymorphism of CLPG and MSTN genes, then the association of the SNPs different genotypes and combined genotypes with sheep growth traits were analyzed by the GLM statistical model of SPSS 22.0.Sequencing results showed that the SNP of C/T which called C1 was detected at position 232 bp of the STS sequence in CLPG gene.The SNP of G/A which called M1 was detected in the 3'UTR of MSTN gene.PCR-RFLP analysis showed that two genotypes CC and CT were in C1 site, two genotypes GG and GA were in M1 site.Association analysis revealed that C1 site was significantly or extremely significantly associated with backfat thickness and loin muscle area (P<0.05;P<0.01), M1 site was significantly or extremely significantly associated with body weight, tube girth, backfat thickness and loin muscle area (P<0.05;P<0.01).Meanwhile, the combined genotype was extremely significantly associated with body weight, backfat thickness and loin muscle area (P<0.01).The conclusions indicated that SNPs and combined genotype of CLPG and MSTN genes had effects on growth traits in sheep.C1 and M1 sites could be considered as effective genetic markers for sheep growth traits. 相似文献
19.
Polymorphism analysis of BMPR1B gene by forced RFLP and PCR-SSCP techniques and expression of the mutation in introgressed sheep 总被引：2，自引：0，他引：2
The present study was conducted to screen Kashmir valley sheep with history of prolificacy for the presence of FecB mutation. Forced polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and single strand conformation
polymorphism (SSCP) techniques were employed to detect any polymorphism present in bone morphogenetic protein receptor type
1B (BMPR1B) gene. Further, it was aimed at introgressing the FecB mutation into nonprolific noncarrier sheep. A 140-bp fragment of BMPR1B gene was amplified from isolated genomic DNA and subjected to forced RFLP with restriction enzyme AvaII. Three different RFLP patterns were identified. SSCP analysis showed one-to-one correspondence with RFLP patterns. Sequencing
of the samples showing different patterns revealed that the wild (+) and mutant (B) alleles were different by a single nucleotide
substitution in the form of A109G from wild to mutant allele. It led to change in amino acid from Glutamine (Q) to Arginine
(R) from wild to mutant allele. The mutation was only detected in NARI-Suwarna and their crosses; all Kashmir valley sheep
with prolific history lacked it. The + allele was abundant in the studied population. The FecB mutation was introgressed in nonprolific noncarrier sheep by crossing ewes with NARI-Suwarna rams possessing the mutation.
First generation crossing produced heterozygous (B+) progeny. Some of the F1 heterozygous ewes gave birth to twins when mated to unrelated NARI-Suwarna rams. It showed that FecB mutation was successfully expressing in those crosses. 相似文献
20.
Mingxing CHU Haibin ZHUANG Yingjie ZHANG Mei JIN Xuewei LI Ran DI Guiling CAO Tao FENG Li FANG 《Animal Science Journal》2011,82(1):57-61
The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Small Tail Han and Hu sheep. According to the sequence of exon 1 and 2 of bovine INHBB gene, six pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and 2 of INHBB gene in both high (Small Tail Han and Hu sheep) and low prolificacy breeds (Dorset, Texel and German Mutton Merino sheep) by polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP). Three pairs of primers (primers 1‐1, 1‐2 and 1‐3) were used to amplify the exon 1, and others (primers 2‐1, 2‐2 and 2‐3) to the exon 2. Only the products amplified by primer 2‐3 displayed polymorphism. For primer 2‐3, three genotypes (AA, AB and BB) were detected in Hu sheep and only AA genotype in other breeds. In Hu sheep, frequency of AA, AB and BB genotypes was 0.636, 0.046 and 0.318, respectively. Sequencing revealed 276A > G mutation (based on the amplification region of primer 2‐3) which did not cause any amino acid change because it lay in the 3′ untranslated region. The ewes with genotype BB had 0.58 (P < 0.01) lambs more than those with AA in Hu sheep. 相似文献