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1.
By propagating lodgepole pine (Pinus contorta) cuttings in vivo, we obtained after 7 growing cycles (ca 3.5 years) in a greenhouse, sufficient number of cuttings from most families to establish clonal progeny tests. Twenty-one full-sib families with approximately 20 clones per family were studied for five years. Years when cuttings were set, families within latitude and clones within families differed significantly in rooting percentages, with the variance components 4.2%, 8.2% and 9.5%, respectively. One way to get a frequent and uniform rooting is to take cuttings from non-leading shoots since they have higher rooting percentage than leading shoots. Neither total length of the cuttings nor length of the primary needles were significantly correlated to rooting percentage. With appropriate management of the ortets and the cuttings during rooting, most clones could be included in a cutting propagation program. 相似文献
2.
Shoot anatomical features viz., number of vessel elements, width and length of vessel and fibre elements were studied in relation
to juvenility/maturity of teak (Tectona grandis Linn. f.). The findings show significant differences in these features, which indicates that variation in shoot anatomy can
be used as a reliable indicator of juvenility/maturity in teak. The effect of age of donor plants and application of auxins
on adventitious root formation in single noded leafy stem cuttings of teak was also investigated. Aging of donor trees suppressed
rooting and sprouting of cuttings, but increased callus formation at the base of cuttings. Per cent rooting and per cent sprouting
in cuttings decreased as age of donor plants increased from 2-months to 15-years and up to 30-years. IBA treatment promoted
per cent rooting as well as per cent sprouting, NAA promoted only rooting and it suppressed sprouting. The effect of auxin
treatment on rooting and sprouting of teak stem cuttings varied with the age of donor plants. Auxin and its concentration,
which suppressed rooting and sprouting in cuttings of younger donor plants, actually promoted rooting when cuttings were taken
from more mature donor plants. It appears that the auxin requirement for causing and promoting rooting in teak cuttings increased
with increasing age of donor plants. The higher auxin requirement for causing and promoting rooting in cuttings of older trees
may be due to a decrease in the content on endogenous auxins or decreased sensitivity of aging tissues to rooting promoters
and /or accumulation of inhibitory substances which inhibit rooting. 相似文献
3.
Aging of the donor tree decreased adventitious root formation in shoot cuttings of Tectona grandis Linn. f. (teak). Exogenous application of auxins, i.e., α-naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) has
a significant positive effect on the percentage of rooting. The maximum percent rooting was obtained with 4,000 ppm IBA as
compared to other treatment. Significant increase in root number was recorded in shoot cuttings treated with 4,000 ppm NAA.
The overall rooting response was better in the treatment with IBA rather than with NAA. Further periodic samples (0, 10, 20,
and 30 days) were taken to assess the total soluble sugar, starch, protein, and peroxidase (PER) activity in the rooting zone
of shoot cuttings of teak during adventitious root formation. Application NAA and IBA to shoot cuttings resulted in an increase
in the level of total soluble sugar, starch, protein, and PER-activity in the rooting zone. The stored carbohydrates were
utilized during adventitious root formation. Hence, total soluble sugar and starch contents of cuttings, irrespective of age
of donor plants, decreased with the passage of time in cuttings planted for rooting. Significant fluctuations were observed
in the protein content of cuttings during the time of root induction. There was an increase in the protein content with the
passage of time from the day of planting up to its 20th day, followed by a sharp decline in the protein content of cuttings
at the 30th day of planting, irrespective of the age of donor plants or the treatment of cuttings with auxins. Irrespective
of donor plant age, PER-activity in the cuttings increased from the day of their planting for rooting up to the 20th day,
and then declined at its 30th day of planting. It was interesting to note that PER-activity remained higher at all stages
in the cuttings of 2-month-old seedlings which rooted profusely as compared to the cuttings of 15- and 30-year-old donor plants
those rooted poorly. This study suggested that the exogenously applied NAA and IBA at different concentration seems to activate
sugar metabolism for release of energy, protein and PER-activity which are necessary for cellular division and differentiation
during adventitious root primordium initiation or development in the rooting zone of shoot cuttings. 相似文献
4.
Variable adventitious rhizogenesis of juvenile shoot cuttings was observed in seven half-sib families (TP-1, BAN-1, AN-3,
JR-3, SL-16, UM-3, and UM-5) of Albizia procera Benth. Treatment with IBA invariably promoted sprouting by 8%, adventitious rooting by 218%, root number by 869%, and root
length by 36.4% compared with the control. Interaction between families and IBA treatment significantly enhanced sprouting
and root length in BAN-1, JR-3, UM-3, and UM-5, and root number in all families, but had a significant suppressive effect
on sprouting in AN-3. Treatment with IBA increased family heritability (h
f
2) 1.38-fold for sprouting, 2.86-fold for adventitious rooting, and 10.86-fold for root number, but reduced family heritability
by a factor of 8.25 for root length. Thus, direct genetic effects seem to have strongly affected sprouting and adventitious
rooting, with IBA playing an auxiliary role, but had little effect on root number and length, for which IBA had a dominant
regulatory role. 相似文献
5.
6.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
7.
Effects of genotype, collection date, auxin treatment, crown position and artificial chilling on rooting and subsequent vegetative growth of Cupressus sempervirens L. softwood cuttings taken from 13-year-old plants were evaluated. Three different clones (318, 296 and 22) previously selected for resistance to cypress cortical cancer caused by Seiridium cardinale were used. In all these clones, both the IBA treated and the untreated cuttings showed seasonal periodicity of rooting. Untreated cuttings rooted best when collected in April (up to 49% for the best clone, i.e. 296) and rooted very poorly in July and October. Auxin treatments appeared unable to overcome this periodicity, as the cuttings taken in July and October did not respond to IBA stimulation. However, in January and April, IBA treatments enhanced adventitious root formation of poorly rooting clones (318 and 22), as well as of clone 296, which performed best (89%) when cuttings were treated in April with 1.5% IBA. Cuttings of each clone collected in the lower third of the crown of the donor trees showed a significantly higher rooting percentage, when compared with those from the upper third. A 4-week post-severance chilling treatment (4°C) significantly enhanced the rooting capacity of cuttings taken in January, while a longer chilling treatment (8 weeks) had a negative effect. The subsequent growth of the rooted cuttings was not affected by either the crown position of the severed cuttings, or the genotype. 相似文献
8.
The main objective of our clonal propagation program is the production of high quality timber and faster tree growth. Teak
(Tectona grandis Linn. f.) is an important plantation species in the tropics but the demand of teak timber is increasing globally. Teak planting
stock is still produced from seeds of unselected elite genotypes which may lead to poor stand establishment. The number of
seeds per fruit and their germination ability are also variable and limited by several factors. Therefore an alternative method
i.e., clonal propagation is required for mass multiplication of elite trees. It is an established fact that the clonal propagation
or adventitious root formation in cuttings is markedly affected by several external and internal factors. Considering these,
an experiment was conducted on leafy soft wood shoot cuttings of 3-year-old hedged teak stock plants grown in a hedge garden
to study the effects of branch position and auxin treatment on adventitious root formation. A factorial completely randomized
design was used for experimentation. Coppice shoots were collected from basal, middle and upper positions of the hedge garden;
and mono-nodal leafy cuttings were prepared. These cuttings were treated with different concentrations of IBA and NAA; and
were cultured in a mist chamber for rooting where the relative humidity was maintained at 85 ± 2% with maximum and minimum
day-night temperature at 32 ± 1°C and 26 ± 1°C respectively. After 45 days the cuttings were removed from the rooting medium
and observations were made. It was observed that the rooting parameters viz., percent rooting and percent sprouting, mean
number of leaves, mean number of shoots and length, and mean number of roots and length per cutting were significantly influenced
by the branch position and auxin treatment. Treatment with 4000 ppm IBA increased percent rooting and percent sprouting whereas
NAA suppressed it. Treatment with 4000 ppm IBA also increased the mean number of leaves and shoots, and the length, and the
mean number of roots per cutting. The highest mean number of roots and length were observed in cuttings taken from the branch
emerging at the upper position followed by branches of middle and lower position. Cuttings of branches at a middle position
showed the highest values of percent rooting, percent sprouting, mean number of leaves and shoots and mean shoot length per
cutting. Furthermore, the interactive effect of branch position and auxin treatment on rooting and sprouting response was
also found to be significant. Overall, the findings of this investigation suggest that middle branch position in hedge plants
and 4000 ppm IBA can be made to induce maximum rooting and to produce high quality planting stock material for clonal forestry
program of teak. 相似文献
9.
The influence of seasonal variation, indole-3-butyric acid (IBA) and type of cuttings was examined on induction and growth
of adventitious roots in Bambusa nutans Wall. and Bambusa tulda Roxb. Singlenode culm and culm-branch cuttings from the mature culms were provided with immersion treatment for 24 h of either
water (control) or 2 mM IBA in four different seasons, i.e., spring (mid February), summer (mid May), rainy (mid July), and
winter (mid November) and maintained for two months in the mist chamber at the relative humidity of (70±5)% and the temperature
of (30±2)°C. In B. nutans, adventitious rooting occurred in both types of cuttings in all the seasons with the best rooting in the summer season i.e.,
May (88% in culm cuttings) and the least in winter. On the contrary, adventitious rooting was recorded only in culm cuttings
in spring and summer season in B. tulda. IBA treatment significantly enhanced rooting, root number and root length; registering 14 to 17 times improvement over control
in the best rooting season. Three factor-interactions (season × cutting type × IBA treatment) were significant for rooting
in B. nutans and all characteristics, except sprouting in B. tulda. Thus, single-node culm and culm-branch cuttings in B. nutans and culm cuttings in B. tulda treated with 2 mM IBA during spring (February) to summer (May) season are recommended for their clonal multiplication. 相似文献
10.
Mikko Tikkinen Saila Varis Heli Peltola Tuija Aronen 《Scandinavian Journal of Forest Research》2018,33(3):207-214
In the Nordic countries, Norway spruce (Picea abies) is a major species in tree breeding. In order to facilitate breeding work and availability of highly bred forest regeneration material, the time required for breeding and implementation of results should be shortened. This could be done by accelerating production of clonal material for field testing, and possibly for planting stock, by combining production of rooted cuttings with somatic embryogenesis (SE). This would allow efficient production of numerous plants of the same genotype, with equal age and propagation history between genotypes. In the present work, we studied the rooting potential of cuttings from Norway spruce emblings. Altogether 36 clones from 12 families representing elite breeding materials and ornamental forms were examined under different rooting conditions (container type and rooting media) in 2015 and 2016. Our results show that Norway spruce emblings are good donors for cuttings. Best combination (peat–vermiculite mixture and Plantek 81f containers) resulted in 91% rooting, variation among the tested clones of elite breeding materials being 55–100% per treatment. The rooting variation between families is acceptable for breeding purposes. High rooting (87–96%) of ornamental forms indicates propagation potential with the combination of SE and rooted cuttings. 相似文献
11.
Kiran Kaul 《New Forests》2008,36(3):217-224
The present study investigates optimal conditions for the vegetative propagation of Himalayan yew Taxus wallichiana Zucc., an important medicinal tree, during spring. Effect of four treatments: (a) sex of donor plant (male and female), (b)
age/type of shoot (1, 2, 3 year old, long and dwarf shoots), (c) auxin treatment (IBA and NAA at 0, 0.5, 1.25, 5.0 & 50.0 mM)
and (d) rooting environment (raised beds/polythene bags) on percentage rooting in stem cuttings was studied. Randomized complete
block (RBD) designs were used for experimentation. Rooting ability of cuttings was significantly influenced by all these treatments.
The overall rooting response was higher in long shoot cuttings taken from female tree. Age of shoot also influenced the rooting
response and was highest in 1 year old long shoot cuttings of female tree. Exogenous application of auxin, α-naphthalene acetic
acid (NAA) and indole-3 butyric acid (IBA), had significant positive effect on the percentage rooting. IBA significantly enhanced
the rooting percentage in 1 year old long and dwarf shoots at lower doses and 2 and 3 year old long shoots from female tree
at higher doses. Maximum percent rooting (90% ± 2.8) was obtained with interactive effect of 0.5 mM, NAA (22 h) × 1 year old
long shoot from female tree; followed by the interactive effect of 50 mM IBA (5 s) × 3 year old long shoot from female tree
(83% ± 4.1). Cuttings planted in soil: sand medium in polythene bags showed earlier rooting response (12 weeks) than cuttings
planted in raised nursery beds (24 weeks). Overall, the findings of this study suggest that 0.5 mM IBA treatment is suitable
for enhancing adventitious rooting in 1 year old long and dwarf shoots of male and female trees. IBA at higher doses is suitable
for enhancing the rooting percentage of 2 and 3 year old long shoots from female tree. This study provides a significant lead
towards the development of a simple and inexpensive technique for large scale propagation, aforestation of elite genotypes
and raising of bush type plantation under ex-situ conditions. 相似文献
12.
Effects of stock plant nutrition on growth and subsequent rooting of leafy stem cuttings of the commercially important west African tree Triplochiton scleroxylon K. Schum. were investigated to identify the primary morphological variables influencing adventitious root formation. Potted plants were watered as required with one of four balanced nutrient solutions (0, 15, 63 or 125 mg N l(-1)). Increasing the nutrient supply to stock plants increased growth rate and rate of adventitious root production of subsequently harvested cuttings, but an optimum nutrient regime was not identified. Rooting percentage increased from 27% in cuttings harvested from stock plants receiving a low nutrient supply to 64% for cuttings harvested from stock plants receiving eight times the amount of nutrients extractable from plants under typical field conditions in West Africa. The ability of a cutting to retain its leaf during propagation and the length of the cutting were the primary measured characteristics associated with rooting and accounted for 43% of the variance fitted in the model. The length of the new shoot, total leaf area and within-shoot position (node position) were also significantly associated with rooting. After these characteristics were fitted to the model, the genetic origin (clone) was not statistically significant. We conclude that genetic variation in rooting potential is mediated through variations in morphological and physiological traits, rather than through genetic variation in cell differentiation or related aspects of root initiation. 相似文献
13.
Liliana Muñoz-Gutiérrez J. Jesús Vargas-Hernández Javier López-Upton Marcos Soto-Hernández 《New Forests》2009,38(2):187-196
Taxus globosa (Mexican yew) is a conifer endemic to México and Central America. It produces a substance known as taxol, which is useful
in treatment of ovarian cancer. Because seed production for this dioecious tree is limited, and seed germination is extremely
difficult, the use of cuttings could facilitate propagation of this species. With the intention of massively propagating individuals
selected for taxol content, two trials were established in which the effect of substrate temperature (average temperatures
18 and 23°C), age (i.e., young vs. old shoots) and management of cuttings, as well as clone variation in rooting capacity,
were evaluated. Low temperature favored rooting (53 vs. 34% on average for the two trials); younger shoots rooted three times
(61 vs. 23%) more than mature ones, while basal wounding did not affect rooting capacity. A wide variation was found in rooting
capacity of clones (8–76%), which could be associated with genetic or physiological differences among donor trees. 相似文献
14.
Many plantation tree species are cloned to achieve the growth, disease resistance and wood quality characteristics required
for a successful economic venture. However, clonal propagation is limited by declines in adventitious root formation with
increasing stock plant age. We examined the effects of immediate or delayed IBA application on adventitious root formation
and subsequent root and shoot development of cuttings harvested from 8-year-old clonal hedge plants of Pinus elliottii var. elliottii × P. caribaea var. hondurensis. IBA applied at the time of setting accelerated root formation, elevating the percentage of cuttings with roots at 13 weeks
post-setting from 45 to 78% and from 83 to 93% for a low- and a high-rooting clone, respectively. Final rooting percentages
for the same treatments and clones (78 and 85%, and 88 and 100%, respectively, at 20 weeks post-setting) were not significantly
affected by IBA application. IBA increased the root:shoot ratio of rooted cuttings by decreasing shoot weight compared with
untreated cuttings, without affecting root weight, root length, root surface area or root volume. IBA was only effective when
applied at the time of setting. A simple IBA treatment for cuttings from 8-year-old clonal hedges, by accelerating root production,
has potential for reducing nursery costs and increasing the root system quality of containerised pine cuttings. 相似文献
15.
本文报导西加云杉优树离体复壮与无性繁殖。低温预处理（２℃，１－３０天）促进休眠顶芽破裂和自由生长芽形成。Ｃ型外植体为最佳接种类型。连续继代培羊可改进复壮效应，腋芽和不定芽再生率。目前还未找到有效试管内生根法。组培嫩梢扦插生根率可达７５％以上。大量部分复壮组培苗已成功移入温室，经园艺生理和外源植物生长调节物质处理，可为生产提供大量扦条，实现快速大量繁殖。 相似文献
16.
Juan Majada Celia Martínez-Alonso Isabel Feito Angelo Kidelman Ismael Aranda Ricardo Alía 《New Forests》2011,41(3):399-412
Pinus pinaster Ait. is one of the main forest tree species planted in Spain, Portugal and France. Due to its high economic relevance, there
is considerable interest in developing techniques for vegetative breeding aimed at mass propagation. In this study we present
a mini-propagation protocol in order to define an efficient method to propagate families or clones of P. pinaster. We carried out three experiments using mini-cuttings of 3–5 cm in length with the aim of evaluating the effects of temperature
(4°C vs. 25°C), plant growth regulator (IBA) and shoot age on rooting ability. Percentage of rooted cuttings and morphological
root variables were recorded. The percentage of rooted cuttings per treatment ranged from 68 to 97%. Treatment with IBA significantly
influenced the rooting process at 25°C but not at 4°C. The number of apexes, length, area and volume of roots were all positively
affected by temperature treatment. Shoot age also had a positive effect on rooting capacity of cuttings, with the cuttings
from the youngest shoots (70 days after pruning) having higher rooting percentages, ranging from 84.7 to 98.3%. The use of
juvenile material, good environmental conditions and IBA all benefited the rooting of clonal material, resulting in high rooting
capacity. This study presents an innovative propagation protocol for P. pinaster that can be used as a tool in breeding programs. 相似文献
18.
Hedged stock plants of four full-sib families [27-2 × 27-5, 27-3× 27-1, 27-2 × 27-1, and 27-6 × 27-1 (designated B, G, R, andW)] of loblolly pine (Pinus taeda L.) were fertilizeddailywith a complete nutrient solution containing N at either 10, 25, 40, 55, or 70mg·L–1. May (spring softwood), July(summersoftwood), and January (winter hardwood) terminal stem cuttings were taken fortissue analysis and rooting studies. Spring cuttings rooted in the highestpercentages (59.5%), followed by winter (40.5%), and summer (34.7%). Maximumrooting for spring (70.0%), summer (48.6%), and winter (55.6%) occurred withcuttings taken from hedges that received N at 55mg·L–1. Genetic differences among familieswere evident as families G and W rooted in greater percentages at lower appliedN rates and family B was the best rooting family at the highest applied N rate.When internal N levels were considered families G and W also rooted best at lowinternal N levels, while family B was the best rooting family at high internalNlevels. Neither total nonstructural carbohydrates (TNC) nor any of severalspecific carbohydrates were correlated with rooting and an optimal TNC : Nratiofor rooting was not found. Root counts generally increased with increases inapplied N. Root number was weakly correlated with TNC (R = 0.29, P 0.01) and several specific sugars, but was not correlated with internal N.Totalroot dry weight, total root length, and total root area responded similarly. 相似文献
19.
《Journal of Sustainable Forestry》2013,32(1):63-72
Abstract Graded doses of IBA (200, 400, and 600 ppm), rice bran extract (0.075%, 0.150%, 0.225% and 0.300%, w/v) and their all possible interactions significantly influenced adventitious rhizogenesis in semi-hardwood shoot cuttings of Tectona grandis. Treatments with 600 ppm IBA and 0.150% rice bran extract proved optimum and increased adventitious rooting by 442% and 49%, respectively, over the control. The regression analysis for various characteristics of propagules indicated a direct role of constituent(s) of rice bran in the process of adventitious rhizogenesis, explaining the total observed variations to the extent of 82.34% in sprout length (p < 0.05), 94.26% in rooting percent (p < 0.05), 88.38% in root number (p < 0.1) and 75.25% in root length (p < 0.1) to be a function of exogenous graded doses of rice bran extract. A very strong synergism between IBA and rice bran was also observed, for a combined application of 600 ppm IBA and 0.150% rice bran extract enhanced adventitious rooting by 1735% over the control. Thus, rice bran extract not only emulates auxin but also acts as rooting synergists. 相似文献
20.
Alex M. Diner 《New Forests》1995,9(1):61-65
Tissues of winter-dormant buds were collected from mature Larix decidua, and used for adventitious shoot organogenesis. Tissues of dormant buds merely died on the cytokinin-supplemented, bud-initiation medium. In contrast, buds allowed to flush under greenhouse conditions subsequently yielded approximately two adventitious shoots per bud explant tissue. These shoots elongated normally and several rooted spontaneously. Microbial contamination of in vitro cultures was negligible using explants from buds orgininally collected in a dormant state. 相似文献