共查询到20条相似文献，搜索用时 15 毫秒
1.
Qun Wan Zhengsheng Zhang Meichun Hu Li Chen Dajun Liu Xiao Chen Wei Wang Jing Zheng 《Euphytica》2007,158(1-2):241-247
A genetic linkage map of chromosome 6 was constructed by using 270 recombinant inbred lines originated from an upland cotton
cross (Yumian 1 × T586) F2 population. The genetic map included one morphological (T1) and 18 SSR loci, covering 96.2 cM with an average distance of 5.34 cM between two markers. Based on composite interval mapping
(CIM), QTL(s) affecting lint percentage, fiber length, fiber length uniformity, fiber strength and spiny bollworm resistance
(Earias spp.) were identified in the t1 locus region on chromosome 6. The allele(s) originating from T586 of QTLs controlling lint percentage increased the trait
phenotypic value while the alleles originating from Yumian 1 of QTLs affecting fiber length, fiber length uniformity, fiber
strength and spiny bollworm resistance increased the trait phenotypic value. 相似文献
2.
陆地棉衣分差异群体产量及产量构成因素 总被引：14，自引：5，他引：9
以衣分差异较大的陆地棉品种为材料,构建了包含188个F2单株的作图群体,应用6111对SSR引物对亲本进行了分子标记筛选,结果仅获得了123个多态性位点,其中88个位点构建了总长为666.7 cM、平均距离为7.57 cM的遗传图谱,覆盖棉花基因组的14.9%。通过复合区间作图法对F2单株和F2∶3家系进行QTL检测,共鉴定出了18个控制产量及产量构成因素变异的QTLs,包括2个衣分QTLs、4个子棉产量QTLs、4个皮棉产量QTLs、2个衣指QTLs、3个单株铃数QTLs、2个铃重QTLs和1个子指QTL。 解释的表型变异分别为\{6.9%\}～16.9%、5.6%～16.2%、4.8%～15.6%、7.7%～13.3%、8.2%～11.6%、6.1%～7%和6.6%。不同QTLs在相同染色体区段上的成簇分布表明与产量性状相关的基因可能紧密连锁或一因多效。产量及产量构成因素QTLs的遗传方式主要以显性和超显性效应为主。检测到的主效QTLs可以用于棉花产量及产量构成因素的分子标记辅助选择。 相似文献
3.
陆地棉产量相关性状的QTL定位 总被引：10，自引：0，他引：10
中棉所28和湘杂棉2号分别是以中棉所12×4133和中棉所12×8891配制而成的两个陆地棉强优势杂交种。以其F2为作图群体,筛选6000多对SSR引物,利用两群体间27个共有多态位点,通过JoinMap 3.0软件整合了一张包含245个多态位点、全长1847.81 cM的遗传图谱。利用Win QTLCart 2.5复合区间作图法分别对两群体8个产量相关性状在F2和F2:3中进行QTL定位,在中棉所28群体多环境平均值的联合分析中检测到16个QTL,三环境分离分析中检测到43个QTL;在湘杂棉2号群体分别检测到20个和66个QTL。在A3、D8、D9等染色体上有QTL成簇分布现象,同时在两个群体中发现一些不受环境影响且稳定遗传的QTL。对考察的8个性状在两个群体中发现12对共有QTL,控制果枝数、衣分和籽指的QTL增效基因位点均来源于共同亲本中棉所12。综合分析推测中棉所12的育种价值主要是通过提高后代的结铃性来实现的。研究结果为棉花产量性状的分子设计育种提供了有用的信息。 相似文献
4.
Dao-Hua He Zhong-Xu Lin Xian-Long Zhang Yi-Chun Nie Xiao-Ping Guo Yan-Xin Zhang Wu Li 《Euphytica》2007,153(1-2):181-197
A high-density molecular marker linkage map of cotton based entirely on polymerase chain reaction-based markers is useful
for a marker-assisted breeding program. Four kinds of markers—simple sequence repeats (SSRs), sequence-related amplified polymorphism
(SRAP), random amplified polymorphic DNA (RAPD), and retrotransposon-microsatellite amplified polymorphism (REMAP)—were used
to assay an F2 population from a cross between “Handan208” (Gossypium hirsutum) and “Pima90” (Gossypium barbadense). Sixty-nine F2 plants were used for map construction using 834 SSRs, 437 SRAPs, 107 RAPDs, and 16 REMAPs. Linkage analysis revealed that
1,029 loci could be mapped to 26 linkage groups that extended for 5,472.3 cM, with an average distance between 2 loci of 5.32 cM.
The corresponding 69 F2:3 families were grown, arranged in two replicates, and scored for eight phenotypes. Quantitative trait loci (QTL) analysis
was performed by means of composite interval mapping using WinQtlCart ver 2.0. A total of 52 distinct QTLs were detected:
4 QTLs for lint index, 8 for seed index, 11 for lint yield, 4 for seed cotton yield, 9 for number of seed per boll, 3 for
fiber strength, 5 for fiber length, and 8 for micronaire value. The present map and QTL analysis may provide a useful tool
for breeders to transfer desirable traits from G. barbadense to the mainly cultivated species, G. hirsutum. 相似文献
5.
陆地棉遗传图谱构建及产量和纤维品质性状QTL定位 总被引：13，自引：0，他引：13
利用3 458对SSR引物筛选陆地棉中棉所35和渝棉1号间的多态性引物, 获得173对。以多态性引物检测(渝棉1号×中棉所35)F2群体180个单株的标记基因型, 共获得178个标记位点。构建的遗传连锁图谱包括148个标记, 36个连锁群, 总长1 309.2 cM, 标记间平均距离8.8 cM, 覆盖棉花基因组的29.5%。36个连锁群中的28个分别被定位于20条染色体, 8个连锁群未定位于染色体。以渝棉1号×中棉所35的F2、F2:3群体的产量、纤维品质性状鉴定结果, 利用区间作图方法, 检测到4个产量性状QTL, 即2个衣分(LP)、1个铃重(BW)、1个籽指(SD); 5个纤维品质性状QTL, 即1个纤维长度(FL)、2个纤维比强度(FS)和2个纤维细度(FF)。LP1、BW、SD、FL和FS1被定位于第7染色体, LP2、FS2、FF1和FF2被分别位于第15、21、9和20染色体。5个纤维品质QTL的有利等位基因均来源于渝棉1号。 相似文献
6.
Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton 总被引：21，自引：1，他引：21
Xinlian Shen Wangzhen Guo Qiongxian Lu Xiefei Zhu Youlu Yuan Tianzhen Zhang 《Euphytica》2007,155(3):371-380
The improvement of cotton fiber quality has become more important because of changes in spinning technology. Stable quantitative
trait loci (QTLs) for fiber quality will enable molecular marker-assisted selection to improve fiber quality of future cotton
cultivars. A simple sequence repeat (SSR) genetic linkage map consisting of 156 loci covering 1,024.4 cM was constructed using
a series of recombinant inbred lines (RIL) developed from an F2 population of an Upland cotton (Gossypium hirsutum L.) cross 7235 × TM-1. Phenotypic data were collected at Nanjing and Guanyun County in 2002 and 2003 for 5 fiber quality
and 6 yield traits. We found 25 major QTLs (LOD ≥ 3.0) and 28 putative QTLs (2.0 < LOD < 3.0) for fiber quality and yield
components in two or four environments independently. Among the 25 QTLs with LOD ≥ 3, we found 4 QTLs with large effects on
fiber quality and 7 QTLs with large effects on yield components. The most important chromosome D8 in the present study was
densely populated with markers and QTLs, in which 36 SSR loci within a chromosomal region of 72.7 cM and 9 QTLs for 8 traits
were detected. 相似文献
7.
转基因抗虫棉产量相关性状QTL的分子标记及定位 总被引：1，自引：0，他引：1
采用亚洲棉渐渗的纤维强度突出的陆地棉优质新品系0-153与陆地棉转基因抗虫新品系sGK9708为亲本,构建了F2及F2∶3分离群体。利用3869对SSR引物筛选亲本,得到125对多态性引物。进一步对183个F2群体单株分析得到150个多态性标记位点,其中100个标记位点连锁,构建20个连锁群,共覆盖660 cM,占棉花总基因组的14.67%,每个连锁群平均包含5个标记位点,标记间平均相距6.6 cM,其中13个连锁群确定了对应的染色体。利用F2和F2:3数据,通过复合区间作图,共检测到28个产量及相关因素的QTLs。这些控制产量性状的QTLs只存在于5个连锁群上,成簇分布。与皮棉产量性状有关的2个QTLs,均与其它多个产量相关性状的QTLs在同一个连锁区段内,增效基因遗传效应方向一致,有必要研究其在标记辅助选择中的效果。本研究没有检测到在多世代表现稳定的QTL。因此,需要培育重组自交系,进一步明确产量性状有关QTL的遗传效应。 相似文献
8.
Mapping QTLs of traits contributing to yield and analysis of genetic effects in tetraploid cotton 总被引：16，自引：0，他引：16
Dao-Hua He Zhong-Xu Lin Xian-Long Zhang Yi-Chun Nie Xiao-Ping Guo Chun-Da Feng James McD. Stewart 《Euphytica》2005,144(1-2):141-149
Fiber yield and yield components – including lint index (LI), seed index (SI), lint yield (LY), seed cotton yield (SCY) and
number of seeds per boll (NSPB) – were investigated on the farm of Huazhong Agricultural University in a population of 69
F2 individuals and corresponding F2:3 families derived from a cross between high-fiber-yield Gossypium hirsutum CV Handan 208 and a low-fiber-yield Gossypium barbadense CV Pima 90. On the basis of the genetic map constructed previously from the same population by Lin et al. (Plant Breed.,
2005), quantitative trait locus (QTL) analysis was performed with the software QTL Cartographer V2.0 using composite interval
mapping method (LOD ≥ 3.0). A total of 21 QTLs were identified, which were located in 15 linkage groups. The number of QTLs
per trait ranged from one to seven. Of these QTLs detected, one affecting LI explained 24.3% of phenotypic variation (PV),
five influencing SI explained 16.15–39.21% of PV, seven controlling LY explained 13.01–28.35% of PV, and two controlling SCY
explained 22.76 and 39.97% of PV, respectively. Simultaneously, the detected six QTLs for NSPB were located on five linkage
groups, which individually explained 28.01–38.32% of the total phenotypic variation. The results would give breeders further
insight into the genetic basis of fiber yield. 相似文献
9.
不同遗传背景下陆地棉衣分和子指性状QTL定位 总被引：3，自引：1，他引：2
为陆地棉产量性状有关的分子标记辅助育种奠定理论基础,以高品质中长绒棉品种‘新陆早24号’为父本,转基因抗虫棉常规品种‘鲁棉研28号’和高产、优质棉花新品种‘冀棉516’为母本,构建F2和F2:3分离群体;利用7638对SSR引物对‘鲁棉研28号’和‘新陆早24号’进行多态性进行筛选,共获得225对多态性引物,对238个F2单株DNA扩增获得238个多态性标记位点,其中185个构建了包括44个连锁群,总长为1509.38 cM的遗传连锁图谱,标记间的平均距离为8.16 cM,覆盖棉花总基因组的33.91%。根据已有图谱的定位结果,40个连锁群与染色体建立联系。利用复合区间作图法定位‘鲁棉研28号’与‘新陆早24号’分离群体F2单株和F2:3家系的衣分和子指性状QTL,其中得到3个衣分和5个子指的QTL;根据定位结果,选择了14对SSR引物,分析‘冀棉516’与‘新陆早24号’的多态性,其中6个标记构建了两个连锁群。1个衣分和1个子指的QTL在两个群体中均检测到,这些共同QTLs为分子标记辅助育种奠定了基础。 相似文献
10.
Jiwen Yu Shuxun Yu Michael Gore Man Wu Honghong Zhai Xingli Li Shuli Fan Meizhen Song Jinfa Zhang 《Euphytica》2013,191(3):375-389
The most widely grown tetraploid Gossypium hirsutum and G. barbadense differ greatly in yield potential and fiber quality and numerous quantitative trait loci (QTLs) have been reported. However, correspondence of QTLs between experiments and populations is poor due to limited number of markers, small population size and inaccurate phenotyping. The purpose of the present study was to map QTLs for yield, yield components and fiber quality traits using testcross progenies between a large interspecific F2 population and a commercial cotton cultivar as the tester. The results were compared to these from its F2 and F2:3 progenies. Of the 177 QTLs identified from the three populations, 65 fiber QTLs and 51 yield QTLs were unique with an average of 8–12 QTLs per traits. All the 26 chromosomes carried QTLs, but differed in the number of QTLs and the number of QTLs between fiber and yield QTLs. The congruence of QTLs identified across populations was higher (20–60 %) for traits with higher heritabilities including fiber quality, seed index and lint percentage, but lower (10–25 %) for lower heritability traits-seedcotton and lint yields. Major QTLs, QTL clusters for the same traits and QTL ‘hotspots’ for different traits were also identified. This research represents the first report using a testcross population in QTL mapping in interspecific cotton crosses and provides useful information for further comparative analysis and marker-assisted selection. 相似文献
11.
陆地棉高品质品系纤维品质性状QTL的分子标记及定位 总被引：4，自引：1，他引：3
为进一步挖掘利用高品质品系NM03102的优异纤维品质性状的基因,利用陆地棉鲁棉研21作为母本、NM03102为父本构建了F2和F2∶3分离群体。通过7892对SSR引物对亲本进行筛选,获得222对多态性引物,进一步对195个F2群体单株分析得到242个标记位点。其中,182个标记位点连锁构建37个连锁群,共覆盖1661.6 cM,每个连锁群平均包含4.9个标记位点,标记间平均相距9.1 cM,其中35个连锁群被定位到了20条染色体上。利用F2和F2∶3纤维品质数据,通过复合区间作图法,共检测到20个纤维品质性状QTL。其中,1个纤维强度的QTL和1个纤维整齐度的QTL与已有的报道一致,1个纤维强度的QTL和1个麦克隆值的QTL在两世代中稳定存在,这为标记辅助选择奠定了基础。 相似文献
12.
【目的】定位棉花纤维品质性状相关的数量性状位点(Quantitative trait locus,QTL)。【方法】以陆地棉高强纤维品系中棉所679和纤维品质一般的农垦5号为亲本构建包含200个单株的F2群体及对应的F2:3家系群体,对2个群体的纤维长度、断裂比强度等5个纤维品质性状进行检测。用6 688对简单重复序列(Simple sequence repeat, SSR)引物在双亲间筛选,得到149对多态性引物,以F2为作图群体,使用QTL IciMapping软件进行连锁图谱构建,并对F2及F2:3群体进行QTL定位。【结果】根据F2群体基因型信息构建了1张包含119个标记、28个连锁群、总长为1 173.5 cM(centiMorgan)的遗传连锁图谱。分别在F_2、F2:3群体中检测到9个和11个与纤维品质性状相关的QTLs,这些QTLs分布在11个连锁群上。其中F2群体的qFL-D11-1、q BT-D11-1与F2:3群体的qFL-D11-1、q MIC-D11-1均定位在标记DPL0062与HAU0423之间,推测这些位点可能是控制纤维品质性状的重要QTL。【结论】利用多个群体进行QTL定位有益于发现稳定的QTL位点,控制纤维品质性状的基因可能成簇存在,为挖掘纤维品质性状相关基因及分子标记辅助育种奠定基础。 相似文献
13.
Furong Wang Yongchao Gong Chuanyun Zhang Guodong Liu Liuming Wang Zhenzhen Xu Jun Zhang 《Euphytica》2011,181(1):41-53
The germplasm with exotic genomic components especially from Sea Island cotton (Gossypium barbadense L. Gb) is the dominant genetic resources to enhance fiber quality of upland cotton (G. hirsutum L., Gh). Due to low efficiency of phenotypic evaluation and selection on fiber quality, genetic dissection of favorable alleles
using molecular markers is essential. Genetic dissection on putative Gb introgressions related to fiber traits were conducted by SSR markers with mapping populations derived from a cross between
Luyuan343 (LY343), a superior fiber quality introgression line (IL) with genomic components from Gb, and an elite Upland cotton cv. Lumianyan#22 (LMY22). Among 82 polymorphic loci screened out from 4050 SSRs, 42 were identified
as putative introgression alleles. A total of 29 fiber-related QTLs (23 for fiber quality and six for lint percentage) were
detected and most of which clustered on the putative Gb introgression chromosomal segments of Chr.2, Chr.16, Chr.23 and Chr.25. As expected, a majority of favorable alleles of fiber
quality QTLs (12/17, not considering the QTLs for fiber fineness) came from the IL parent and most of which (11/12) were conferred
by the introgression genomic components while three of the six (3/6) favorable alleles for lint percentage came from the Gh parent. Validation of these QTLs using an F8 breeding population from the same cross made previously indicated that 13 out of 29 QTLs showed considerable stability. The
results suggest that fiber quality improvement using the introgression components could be facilitated by marker-assisted
selection in cotton breeding program. 相似文献
14.
定位棉花种子性状的基因对揭示棉花种子性状的遗传规律,以及明确棉花种子、产量、纤维品质等性状间的遗传关系具有重要意义。以(渝棉1号×T586) F2:7重组近交系群体构建的遗传连锁图谱,在鉴定270个家系3个环境种子物理性状的基础上,利用MQM作图方法,共检测到34个种子物理性状QTL,包括9个种子重(qSW)、5个短绒重(qFW)、3个短绒率(qFP)、8个种仁重(qKW)、6个种子壳重(qHW)和3个种仁率(qKP)QTL,它们可解释4.6%~80.1%的性状表型变异。9个QTL在2个或3个环境中被检测到,其中包括第12染色体显性光子位点的短绒重与短绒率QTL,以及另外7个微效应QTL。34个QTL分布于15条染色体,其中A染色体组20个,D染色体组14个。有12个染色体区段分布有2个或2个以上的QTL,而且同一染色体区域同一亲本所具有的不同性状QTL的方向大多数与性状表型相关系数的正负一致。 相似文献
15.
Tomoko Hashida Ryoichi Nakatsuji Holger Budahn Otto Schrader Herbert Peterka Tatsuhito Fujimura Nakao Kubo Masashi Hirai 《Breeding Science》2013,63(2):218-226
The radish displays great morphological variation but the genetic factors underlying this variability are mostly unknown. To identify quantitative trait loci (QTLs) controlling radish morphological traits, we cultivated 94 F4 and F5 recombinant inbred lines derived from a cross between the rat-tail radish and the Japanese radish cultivar ‘Harufuku’ inbred lines. Eight morphological traits (ovule and seed numbers per silique, plant shape, pubescence and root formation) were measured for investigation. We constructed a map composed of 322 markers with a total length of 673.6 cM. The linkage groups were assigned to the radish chromosomes using disomic rape-radish chromosome-addition lines. On the map, eight and 10 QTLs were identified in 2008 and 2009, respectively. The chromosome-linkage group correspondence, the sequence-specific markers and the QTLs detected here will provide useful information for further genetic studies and for selection during radish breeding programs. 相似文献
16.
鲁棉研15号纤维品质性状QTL定位研究 总被引：3，自引：3，他引：0
以陆地棉（Gossypium hirsutum L.）杂交种鲁棉研15号的F2群体为作图群体,利用SSR标记和JoinMap3.0软件构建遗传连锁图谱;利用复合区间作图法分别对随机组成的3个鲁棉研15号的F2:3家系亚群体进行纤维品质性状QTL定位。构建的遗传连锁图谱包含116个多态位点,25个连锁群,全长892.25 cM,覆盖棉花总基因组的20.05%,平均每个连锁群4.64个标记,标记间平均距离7.76 cM;根据已有图谱的定位结果,19个连锁群与染色体建立了联系。在3个F2:3家系亚群体中共检测到46个QTL,其中16个为纤维长度（FL）QTL、7个为纤维强度（FS）、12个为麦克隆值（FM）、6个为伸长率（FE）,5个为整齐度指数（FU）。发现在Ah05、Ah08、Ah09、Dh02染色体上QTL有成簇分布的现象,并在3个亚群体中检测到一些受环境影响较小、稳定遗传的QTL。这些QTL可以在今后应用于分子标记辅助选择。 相似文献
17.
Quantitative analysis and QTL mapping for agronomic and fiber traits in an RI population of upland cotton 总被引：3，自引：1，他引：2
Jixiang Wu Osman Ariel Gutierrez Johnie N. Jenkins Jack C. McCarty Jun Zhu 《Euphytica》2009,165(2):231-245
Genetic mapping is an essential tool for cotton (Gossypium hirsutum L.) molecular breeding and application of DNA markers for cotton improvement. In this present study, we evaluated an RI population
including 188 RI lines developed from 94 F2-derived families and their two parental lines, ‘HS 46’ and ‘MARCABUCAG8US-1-88’, at Mississippi State, MS, for two years.
Fourteen agronomic and fiber traits were measured. One hundred forty one (141) polymorphic SSR markers were screened for this
population and 125 markers were used to construct a linkage map. Twenty six linkage groups were constructed, covering 125
SSR loci and 965 cM of overall map distance. Twenty four linkage groups (115 SSR loci) were assigned to specific chromosomes.
Quantitative genetic analysis showed that the genotypic effects accounted for more than 20% of the phenotypic variation for
all traits except fiber perimeter (18%). Fifty six QTLs (LOD > 3.0) associated with 14 agronomic and fiber traits were located
on 17 chromosomes. One QTL associated with fiber elongation was located on linkage group LGU01. Nine chromosomes in sub-A
genome harbored 27 QTLs with 10 associated with agronomic traits and 17 with fiber traits. Eight chromosomes in D sub-genome
harbored 29 QTLs with 13 associated with agronomic traits and 16 with fiber traits. Chromosomes 3, 5, 12, 13, 14, 16, 20,
and 26 harbor important QTLs for both yield and fiber quality compared to other chromosomes. Since this RI population was
developed from an intraspecific cross within upland cotton, these QTLs should be useful for marker assisted selection for
improving breeding efficiency in cotton line development.
Paper number J1116 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi
State, MS 39762. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product
by USDA, ARS and does not imply its approval to the exclusion of other products or vendors that may also be suitable. 相似文献
18.
Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.) 总被引：13，自引：0，他引：13
Summary Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) × SD35 (intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non-radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits. 相似文献
19.
An extensive genetic linkage map was constructed for bitter gourd (Momordica charantia L.) via the study of F2 progenies derived from two cultivated inbred lines (gynoecia Z-1-4 and 189-4-1). The map included 194 loci on 11 chromosomes consisting of 26 EST-SSR loci, 28 SSR loci, 124 AFLP loci, and 16 SRAP loci. This map covered 1005.9 cM with 12 linkage groups. A total of 43 quantitative trait loci (QTLs), with a single QTL associated with 5.1–33.1 % phenotypic variance, were identified on nine chromosomes for 13 horticulture traits by analyzing the F2-3 families and the genetic linkage map. The 13 horticulture traits which were investigated in three environments included female flower ratios (FFR), first female flower node (FFFN), fruit length, fruit diameter, flesh thickness, fruit shape, fruit pedicel length, fruit length pedicel ratios, fruit weight (FW), fruit numbers per plant (FPP), yield per plant (YPP), stem diameter (SD), and internodes length (IL). One QTL cluster region was detected on Lg-5 which contained the most important QTLs for YPP, FPP, FFFN, FFR, and FW with high contributions to phenotypic variance (5.8–25.4 %). 相似文献
20.
棉花BC_5F_2代换系的产量及品质相关性状表型分析及QTL定位 总被引：7，自引：0，他引：7
本研究利用生产上推广的优良早熟陆地棉栽培品种中棉所36为受体亲本,海岛棉海1为供体亲本,选择培育了一套由303个单株组成的BC5F2代换系。从已构建的BC1F1遗传图谱上以5~10cM为标准挑选391对多态性标记进行分子检测,多数单株含有海岛棉代换片段数为2~10个。对该群体的单株产量、品质性状进行了表型鉴定,存在大量具有优良品质的单株,纤维强度最高的能达到37.8cN/tex,铃重、衣分、纤维长度、整齐度、马克隆值、伸长率及纤维强度超轮回亲本分别为17.82%、44.55%、46.86%、33.33%、74.92%、41.58%、42.57%。采用性状-标记间的单向方差分析,共定位了20个与产量性状和33个与纤维品质性状有关的QTL,其中qUN-14-2、qBW-2-20、qFL-2-20和qMV-1-38这4个QTL存在一定的遗传稳定性。鉴定的QTL大多是微效基因,解释表型变异为3.01%~9.69%,该研究为染色体单片段代换系的精细的分子研究奠定了基础。 相似文献