共查询到19条相似文献，搜索用时 120 毫秒
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一个水稻叶片白化转绿叶突变体的遗传分析和精细定位 总被引：5，自引：0，他引：5
在水稻品种宜香B中发现了一个白化转绿叶突变体,经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色,之后逐渐变绿,从苗龄4周到12周,突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变受1对隐性核基因控制,暂命名为gra。利用微卫星标记将gra初步定位于第10染色体标记RM596和RM5620之间,进一步利用极端个体定位法把gra精细定位于标记RM25522和RM25535之间。gra基因距RM25522和RM25535标记的遗传距离均为0.05 cM, 其物理距离约为136 kb。 相似文献
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为保证设施叶用莴苣周年生产的供给,研究了人工气候条件下不同光照模式对叶用莴苣生长期生长特性的影响,以确定叶用莴苣生长的最优光环境,实验以‘意大利’全年耐抽薹叶用莴苣（Lactuca sativa L.）为试材,设计 LED 红蓝配比（7:3、5:5、3:7）、光周期（12L/12D、16L/8D、20L/4D）及光照强度[100 μmol/(m2·s)、150 μmol/(m2·s)、200 μmol/(m2·s)]的 3 因素 3 水平正交实验,利用灰色关联分析法分析不同光照模式对生长期叶用莴苣植株的生长、光合色素含量及营养品质的影响。结果表明,不同处理对叶用莴苣生长期生长及品质的影响不同。相比于其他光环境条件,处理 9[红蓝配比 3:7,光周期 20L/4D,光强 150 μmol/(m2·s)]更有利于光合色素的积累。红蓝配比 3:7 的光照模式较其他处理更有利于蛋白质合成;处理 3[红蓝配比 7:3,光周期 20L/4D,光强 200 μmol/(m2·s)]有利于降低生长期叶用莴苣叶片硝态氮含量,促进可溶性糖的积累。综合分析得出 3 个光照因子对水培叶用莴苣生长及品质影响的重要程度依次为：光强、光质配比、光周期,最适的光照模式组合为：红蓝配比 7:3、光强 200 μmol/(m2·s)、光周期 20L/4D。本研究结果可为叶用莴苣生长的最适光环境提供参考,同时为 LED 灯具在植物照明领域的设计提供
技术支持。 相似文献
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研究了短根突变体RM1、RM2及其原品种Ohchikara在水培和土培条件下的农艺性状。结果表明，用木村氏B溶液水培，从播种后14 d至成熟，突变体冠根的长度、鲜重和干重分别只有原品种的50%、50%和60%。从播后60 d至成熟，突变体的冠根数是原品种的1.2倍，在这之前它们之间无显著差异。在所有调查的地下部性状中，RM1和RM2之间无显著差异。在地上部的株高、分蘖数、地上部鲜重、干重及全株干重性状上，两个突变体与原品种之间、RM1和RM2之间无显著差异。突变体的全株鲜重为原品种的90%，这个差异是显著的。突变体的单株粒重只有原品种的60%，这是由于每穗总粒数和结实率下降所致。在大田土培条件下，所有调查过的地上部性状值，突变体都显著地小于原品种，而RM1和RM2之间除穗长外，均无显著差异。 相似文献
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随着作物设施栽培技术的发展,遮荫与补光栽培成为提高作物产量与品质的重要手段。本研究以胡椒扦插苗为材料,采用室内营养液浇灌法培养,研究不同光照强度环境对胡椒扦插苗抽穗生理过程的影响。结果表明：在135 μmol/(m 2·s)光强环境下胡椒叶片的光合速率相对较低,植株同化的生物量较少,花芽抽生少,叶片厚且单叶面积较大;270 μmol/(m 2·s)光强环境下,胡椒的光合速率维持较高水平;405 μmol/(m 2·s)光照强度下,胡椒扦插苗成熟叶片中可溶性糖、蔗糖、淀粉等糖类物质含量与赤霉素水平较高,叶片抽生数多,净增生物量最大,胡椒成花数多;540 μmol/(m 2·s)光照环境下,胡椒扦插苗生长发育受到轻微抑制;相关性分析表明可溶性糖、果糖、GA3含量与胡椒抽穗量成显著相关。此次研究结果表明：胡椒扦插苗在405 μmol/(m 2·s)光强环境下糖类物质含量与赤霉素水平相对较高,最有利于胡椒多抽穗。本研究可以为我国胡椒控光栽培技术的改进提供理论参考。 相似文献
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水稻扭曲叶突变体rtl1是利用甲基磺酸乙酯（EMS）诱变粳稻品种日本晴获得的。在苗期,该突变体的叶片就表现出皱缩和扭曲状。将该突变体分别与籼稻品种台中本地1号和浙辐802进行配组。遗传分析表明该突变体性状受1对隐性单基因控制。通过混合分离法,找到了位于第4染色体上的紧密连锁SSR标记RM1155,通过新发展的多态性STS标记,最终将该基因定位在STS标记T1591和SSR标记RM1359之间,其遗传距离分别为0.48和0.96 cM。为进一步克隆该基因打下了基础。 相似文献
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一个新的水稻叶绿素缺失突变基因的遗传分析和分子标记定位 总被引：4，自引：0，他引：4
从正常绿色水稻品种824B中发现1个黄化突变体824ys。该突变体具有叶绿素缺失突变特性,表现为植株黄绿色,分蘖数减少,生育期延长,总叶绿素、叶绿素a、叶绿素b的含量以及净光合速率比野生型亲本824B明显下降,每穗着粒数、结实率、千粒重等降低。对824ys与3个正常绿色品种杂交F1、F2的遗传分析表明,控制824ys的叶绿素缺失突变性状为1对隐性核基因。以495R/824ys F2作为定位群体,应用微卫星标记将824ys的叶绿素缺失突变基因定位于水稻第3染色体短臂,与RM218、RM282和RM6959等标记之间的遗传距离分别为25.6、 5.2和21.8 cM。认为该基因为一个新的水稻叶绿素缺失突变基因,暂命名为chl11(t)。 相似文献
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The light-sensitive red-root mutant, designated as HG1, was newly observed from an indica rice variety, Nankinkodo, when seedlings were grown with roots exposed to natural light. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 μmol/(m2·s), then turned dark-red at the light intensity of 180 μmol/(m2·s), suggesting that the root color of the mutant was evidently sensitive to light. Furthermore, genetic analysis showed that the character of ... 相似文献
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一个水稻新黄绿叶突变体基因的分子定位 总被引：17，自引：1，他引：16
在水稻品种武运粳7号中发现了一个黄绿叶自然突变体,经过多代自交形成了稳定的突变系。该突变系和武运粳7号的正反交F2代的遗传分析表明该材料的黄绿叶由1对隐性基因控制,命名为 ygl 2。利用已有的微卫星（SSR）标记和新发展的SSR标记将 ygl 2基因定位于RM1340、RM7269、RM6298、SSR6 16和RM7434、SSR6 5、SSR6 9、RM5957之间,排列位置为RM1340-RM7269-RM6298-SSR6 16 -ygl 2-RM7434-SSR6 5、SSR6 9-RM5957,它们之间的遗传距离分别为238、0.37、0.00、0.62、0.74、0.49、0.86和1.62 cM,这为 ygl 2基因的分子标记辅助选择育种和图位克隆奠定了基础。 相似文献
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LI Jin-bo XIA Ming-yuan WAN Bing-liang DU Xue-shu ZHA Zhong-ping YU Da-zhao QI Hua-xiong 《水稻科学》2009,16(1):79-82
A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene. 相似文献
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A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B.It showed whole green-yellow plant from the seedling stage,reduced number of tillers and longer growth duration.The contents of chlorophyll,chlorophyll a,chlorophyll b and net photosynthetic rate in leaves of the mutant obviously decreased,as well as the number of spikelets per panicle,seed setting rate and 1000-grain weight compared with its wild-type parent.Genetic analyses on F1 and F2 generetions of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by a pair of recessive nuclear gene.Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys,and the mutant gene of 824ys was mapped on the shon arm of rice chromosome 3.The genetic distances from the target gene to the markers RM218,RM282 and RM6959 were 25.6 cM,5.2 cM and 21.8 cM,respectively.It was considered to be a now chlorophyll-deficit mutant gene and tentatively named as chl11(t). 相似文献
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稻米蒸煮品质性状的QTL定位 总被引：5，自引：1，他引：4
以D50/HB277衍生的由190个家系组成的F7重组自交系群体为研究材料,构建了包含102个SSR标记的遗传连锁图谱,覆盖水稻基因组1226.4 cM区域,平均图距为12.02 cM。利用Windows QTL Cartographer 2.5软件,对蒸煮品质进行QTL分析。在杭州和海南两地实验中,共检测到6个具有显著加性效应的QTL。其中,分别控制直链淀粉含量和胶稠度的主效QTL（qAC6和qGC6）均位于第6染色体短臂RM508和RM587之间,控制碱消值的主效QTL（qASV6）位于第6染色体短臂RM111和RM3438之间,且均能在两地稳定表达。 相似文献
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FANG Li-kui SANG Xian-chun YANG Zheng-lin LIN Ying-hua WAN Nan HE Guang-hua 《水稻科学》2009,16(4):323-326
Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller angle controlling 2 (tac2) mutant was obtained from a restorer line Jinhui 10 by ethyl methane sulphonate mutagenesis. The tac2 mutant displayed normal phenotype at the seedling stage and the tiller angle significantly increased at the tillering stage. A preliminary physiological research indicated that the mutant was sensitive to GA. Thus, it is speculated that TAC2 and TAC1 might control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene and was located on chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 cM and 16.7 cM, respectively. 相似文献
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水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位 总被引：1，自引：0，他引：1
通过EMS诱变籼稻品种IR64获得一个稳定遗传的淡褐色斑点叶突变体lbsl1（light brown spotted leaf 1）。在自然条件下,突变体播种后10~14 d,叶片上出现淡褐色斑点,随后逐渐扩散至全叶,第1叶至剑叶上均有淡褐色斑,为全生育期性状。斑点性状的表达对株高、生育期、结实率和千粒重等农艺性状具有显著的影响。遗传分析结果表明,该淡褐色斑点叶性状受一个隐性核基因控制。将突变体lbsl1与正常叶色水稻Morobereken杂交构建F2定位群体,利用SSR标记,最终将该淡褐叶基因lbsl1(t)定位在第6染色体短臂上一个约130 kb的区段上。定位的结果和发展的群体为该基因的进一步精细定位和克隆奠定了基础。 相似文献
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HUANG Ting-you WANG Zhi HU Yun-gao SHI Shou-pei PENG Tao CHU Xu-dong SHI Jun XIANG Zu-fen LIU Ding-you 《水稻科学》2008,15(2):153-156
To understand the genetic characteristics of a new photoperiod-sensitive genic male sterile line Mian 9S,some reciprocal crosses were made between Mian 9S and six indica rice materials,Yangdao 6,Luhui 602,Shuihui 527,Mianhui 725,Fuhui 838 and Yixiang 1B.Genetic analysis results suggested that the photoperiod-sensitive genic male sterility (PGMS) of Mian 9S was controlled by a single recessive nuclear gene.Thus,the F2 population derived from the cross of Yangdao 6/Mian 9S was used to map the PGMS gene in Mian 9S.By using SSR markers,the PGMS gene of Mian 9S was mapped on one side of the markers,RM6659 and RM1305,on rice chromosome 4,with the genetic distances of 3.0 cM and 3.5 cM,respectively.The gene was a novel PGMS gene and designated tentatively as pros4.In addition,the application of the pros4 gene was discussed. 相似文献
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LIu Ming-wei LIU Yong WANG Shi-quan DENG Qi-ming LI Ping 《水稻科学》2005,12(4):243-248
Grain size, determined chiefly by grain length, is one of the main factors affecting the grain yield in rice production. To study the trait of rice grain size, F1 and F2 populations were developed from crosses Shuhui 881/Y34 and Shuhui 527/Y34, and genetic analysis for minute grain was performed. The F1 populations showed minute grains, and grain size segregations in the two F2 populations were both in accordance with the ratio of 3：1, indicating that minute grain in Y34 was controlled by a completely dominant gene. By using the F2 population from Shuhui 881/Y34, this dominant gene, tentatively designated as Mi3（t）, was mapped based on SSR markers in the interval between RM282 （genetic distance of 5.1 cM） and RM6283 （genetic distance of 0.9 cM） on the short arm of chromosome 3. 相似文献