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1.
A combination of stable isotope and acetylene (0.01% v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha–1) silt loam soil at contrasting (20–70%) water-filled pore space (WFPS). 15N-N2O emissions from 14NH415NO3 replicates were attributed to denitrification and 15N-N2O from 15NH415NO3 minus that from 14NH415NO3 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of 15N-NO3–. All of the N2O emitted at 70% WFPS (31.6 mg N2O-N m–2 over 24 days; 1.12 g N2O-N g dry soil–1; 0.16% of N applied) was produced during denitrification, but at 35–60% WFPS nitrification was the main process producing N2O, accounting for 81% of 15N-N2O emitted at 60% WFPS, and 7.9 g 15N-N2O m–2 (0.28 ng 15N-N2O g dry soil–1) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50% WFPS treatment and accounted for 20% of 15N-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20% WFPS (2.6 g 15N-N2O m–2 over 7 days; 0.09 ng 15N-N2O g dry soil–1; 85% of 15N-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.  相似文献   

2.
Soil N2O emissions can affect global environments because N2O is a potent greenhouse gas and ozone depletion substance. In the context of global warming, there is increasing concern over the emissions of N2O from turfgrass systems. It is possible that management practices could be tailored to reduce emissions, but this would require a better understanding of factors controlling N2O production. In the present study we evaluated the spatial variability of soil N2O production and its correlation with soil physical, chemical and microbial properties. The impacts of grass clipping addition on soil N2O production were also examined. Soil samples were collected from a chronosequence of three golf courses (10, 30, and 100-year-old) and incubated for 60 days at either 60% or 90% water filled-pore space (WFPS) with or without the addition of grass clippings or wheat straw. Both soil N2O flux and soil inorganic N were measured periodically throughout the incubation. For unamended soils, cumulative soil N2O production during the incubation ranged from 75 to 972 ng N g−1 soil at 60% WFPS and from 76 to 8842 ng N g−1 soil at 90% WFPS. Among all the soil physical, chemical and microbial properties examined, soil N2O production showed the largest spatial variability with the coefficient of variation ~110% and 207% for 60% and 90% WFPS, respectively. At 60% WFPS, soil N2O production was positively correlated with soil clay fraction (Pearson's r = 0.91, P < 0.01) and soil NH4+–N (Pearson's r = 0.82, P < 0.01). At 90% WFPS, however, soil N2O production appeared to be positively related to total soil C and N, but negatively related to soil pH. Addition of grass clippings and wheat straw did not consistently affect soil N2O production across moisture treatments. Soil N2O production at 60% WFPS was enhanced by the addition of grass clippings and unaffected by wheat straw (P < 0.05). In contrast, soil N2O production at 90% WFPS was inhibited by the addition of wheat straw and little influenced by glass clippings (P < 0.05), except for soil samples with >2.5% organic C. Net N mineralization in soil samples with >2.5% organic C was similar between the two moisture regimes, suggesting that O2 availability was greater than expected from 90% WFPS. Nonetheless, small and moderate changes in the percentage of clay fraction, soil organic matter content, and soil pH were found to be associated with large variations in soil N2O production. Our study suggested that managing soil acidity via liming could substantially control soil N2O production in turfgrass systems.  相似文献   

3.
It is increasingly believed that substantial soil organic carbon (SOC) can be sequestered in conservation tillage system by manipulating the functional groups of soil biota. Soil aggregates of different size provide diverse microhabitats for soil biota and consequently influence C sequestration. Our objective was to evaluate the contributions of soil biota induced by tillage systems to C sequestration among different aggregate size fractions. Soil microbial and nematode communities were examined within four aggregate fractions: large macroaggregates (>2 mm), macroaggregates (2–1 mm), small macroaggregates (1–0.25 mm) and microaggregates (<0.25 mm) isolated from three tillage systems: no tillage (NT), ridge tillage (RT) and conventional tillage (CT) in Northeast China. Soil microbial and nematode communities varied across both tillage systems and aggregate fractions. The activity and abundance of microbes and nematodes were generally higher under NT and RT than under CT. Among the four aggregate fractions, soil microbial biomass and diversity were higher in microaggregates, while soil nematode abundance and diversity were higher in large macroaggregates. Structural equation modelling (SEM) revealed that the linkage between microbial and nematode communities and their contributions to soil C accumulation in >1 mm aggregate fractions were different from those in <1 mm aggregate fractions. Higher abundance of arbuscular mycorrhizal fungi (AMF) could enhance C retention within >1 mm aggregates, while more gram-positive bacteria and plant-parasitic nematodes might increase C accumulation within <1 mm aggregates. Our findings suggested that the increase in microbial biomass and nematode abundance and the alteration in their community composition at the micro-niche within aggregates could contribute to the higher C sequestration in conservation tillage systems (NT and RT).  相似文献   

4.
Soil aggregation is influenced by the tillage system used, which in turn affects the amount of C and N in the different aggregate fractions. This study assessed the impact of different tillage systems on soil aggregates by measuring the aggregate stability, the organic carbon (Corg) and the total nitrogen (Ntot) contents within different aggregate fractions, and their release of dissolved organic carbon (DOC). Soil samples were collected from the top 0 to 10 cm of a long-term tillage experiment at Fuchsenbigl (Marchfeld, Austria) where conventional tillage (CT), reduced tillage (RT), and minimum tillage (MT) treatments were applied to a Chernozem fine sandy loam. The stable aggregates (1000–2000 μm) were subject to dispersion by the soil aggregate stability (SAS or wet sieving) method after Kemper and Rosenau (1986), and the ultrasonic method of Mayer et al. (2002). Chemical analysis of the soil was obtained for the aggregate fractions 630–1000, 250–630 and 63–250 μm gathered from the ultrasonic method. Using the SAS method, CT and RT had the least amounts of stable aggregates (18.2% and 18.9%, respectively), whereas MT had twice as much stable aggregates (37.6%). Using the ultrasonic method, MT also had the highest amount of water stable aggregates in all three fractions (1.5%, 3.7%, and 35%, respectively), followed by RT (1%, 2.3%, 32.3%), and CT (0.8%, 1.7%, 29.1%). For comparison, a reference soil, EUROSOIL 7 (ES-7) was also analysed (40%, 6.7%, and 12.1%). The highest amounts of Corg and Ntot were measured under MT in all three fractions, with 8.9%, 3.8%, and 1.3% for Corg, and 0.4%, 0.3%, and 0.1% for Ntot. Apart from the fraction 630–1000 μm, the aggregates of RT and CT contained <50% of the Corg and Ntot values of MT. The C/N ratio was least favourable for CT (42.6) in the aggregate fraction 630–1000 μm. The DOC release from stable aggregates after 10 min of ultrasonic dispersion was highest from MT soil (86.7 mg l−1). The values for RT and CT were 21% and 25% below this value. The results demonstrate that tillage type influences both aggregate stability and aggregate chemical composition. This research confirms that CT interferes more with the natural soil properties than RT and MT. Furthermore, MT has the highest potential to sequester C and N in this agriculturally used soil.  相似文献   

5.
We studied the effects of soil management and changes of land use on soils of three adjacent plots of cropland, pasture and oak (Quercus robur) forest. The pasture and the forest were established in part of the cropland, respectively, 20 and 40 yr before the study began. Soil organic matter (SOM) dynamics, water-filled pore space (WFPS), soil temperature, inorganic N and microbial C, as well as fluxes of CO2, CH4 and N2O were measured in the plots over 25 months. The transformation of the cropland to mowed pasture slightly increased the soil organic and microbial C contents, whereas afforestation significantly increased these variables. The cropland and pasture soils showed low CH4 uptake rates (<1 kg C ha−1 yr−1) and, coinciding with WFPS values >70%, episodes of CH4 emission, which could be favoured by soil compaction. In the forest site, possibly because of the changes in soil structure and microbial activity, the soil always acted as a sink for CH4 (4.7 kg C ha−1 yr−1). The N2O releases at the cropland and pasture sites (2.7 and 4.8 kg N2O-N ha−1 yr−1) were, respectively, 3 and 6 times higher than at the forest site (0.8 kg N2O-N ha−1 yr−1). The highest N2O emissions in the cultivated soils were related to fertilisation and slurry application, and always occurred when the WFPS >60%. These results show that the changes in soil properties as a consequence of the transformation of cropfield to intensive grassland do not imply substantial changes in SOM or in the dynamics of CH4 and N2O. On the contrary, afforestation resulted in increases in SOM content and CH4 uptake, as well as decreases in N2O emissions.  相似文献   

6.
Few studies address nutrient cycling during the transition period (e.g., 1–4 years following conversion) from standard to some form of conservation tillage. This study compares the influence of minimum versus standard tillage on changes in soil nitrogen (N) stabilization, nitrous oxide (N2O) emissions, short-term N cycling, and crop N use efficiency 1 year after tillage conversion in conventional (i.e., synthetic fertilizer-N only), low-input (i.e., alternating annual synthetic fertilizer- and cover crop-N), and organic (i.e., manure- and cover crop-N) irrigated, maize–tomato systems in California. To understand the mechanisms governing N cycling in these systems, we traced 15N-labeled fertilizer/cover crop into the maize grain, whole soil, and three soil fractions: macroaggregates (>250 μm), microaggregates (53–250 μm) and silt-and-clay (<53 μm). We found a cropping system effect on soil Nnew (i.e., N derived from 15N-fertilizer or -15N-cover crop), with 173 kg Nnew ha−1 in the conventional system compared to 71.6 and 69.2 kg Nnew ha−1 in the low-input and organic systems, respectively. In the conventional system, more Nnew was found in the microaggregate and silt-and-clay fractions, whereas, the Nnew of the organic and low-input systems resided mainly in the macroaggregates. Even though no effect of tillage was found on soil aggregation, the minimum tillage systems showed greater soil fraction-Nnew than the standard tillage systems, suggesting greater potential for N stabilization under minimum tillage. Grain-Nnew was also higher in the minimum versus standard tillage systems. Nevertheless, minimum tillage led to the greatest N2O emissions (39.5 g N2O–N ha−1 day−1) from the conventional cropping system, where N turnover was already the fastest among the cropping systems. In contrast, minimum tillage combined with the low-input system (which received the least N ha−1) produced intermediate N2O emissions, soil N stabilization, and crop N use efficiency. Although total soil N did not change after 1 year of conversion from standard to minimum tillage, our use of stable isotopes permitted the early detection of interactive effects between tillage regimes and cropping systems that determine the trade-offs among N stabilization, N2O emissions, and N availability.  相似文献   

7.
Sharp peaks in nitrous oxide (N2O) fluxes under no-tillage in wet conditions appear to be related to near surface soil and crop cover conditions. Here we explored some of the factors influencing tillage effects on short-term variations in gas flux so that we could learn about the mechanisms involved. Field investigations revealed that a cumulative emission of 13 kg N2O–N ha−1 over a 12-week period was possible under no-tillage for spring barley. We investigated how reducing crop cover and changing the structural arrangement of the water-filled pore space (WFPS) by short-term laboratory compaction influenced N2O and carbon dioxide (CO2) fluxes in upward and downward directions in core samples from tilled and untilled soil. Increasing the downward flux of N2O within a soil profile by changing soil or moisture conditions may increase the likelihood of its further reduction to N2 or dissolution. We took undisturbed cores from 3 to 8 cm depth, equilibrated them to −1 or −6 kPa matric potential, incubated them and measured N2O and CO2 fluxes from the upper and lower surfaces in a purpose-designed apparatus before and after compaction in an uniaxial tester. We also measured WFPS, air permeability, bulk density and air-filled porosity before and after compaction. Spring barley was tested in 1999 and winter barley in 2000.Fluxes of N2O were from 1.5 to 35 times higher from no-tilled than ploughed even where the soil was of similar bulk density. Reduction of the crop cover increased CO2 flux and could reduce N2O flux. The effects of structural changes induced by laboratory compaction on the fluxes of N2O and CO2 were not influenced greatly by the tillage and crop cover treatments. Fluxes from the upper surfaces of cores (corresponding to 3 cm soil depth, upwards direction) could be up to 100 times greater (N2O) or 8 times (CO2) than from the lower surfaces (8 cm depth, downwards direction). These differences between surfaces were greatest when N2O fluxes were very high in no-tilled soil (4.2 mg N2O–N m−2 h−1) as occurred when WFPS exceeded 80% or became blocked with water, an effect that was increased by our compaction treatment. In general N2O fluxes increased with WFPS. The production and emission of N2O were strongly influenced by the soil physical environment, the magnitude of the water-filled pore space and continuity of the air-filled pore space in particular, produced in no-till versus plough cultivation.  相似文献   

8.
Mixed responses of soil nitrous oxide (N2O) fluxes to reduced tillage/no-till are widely reported across soil types and regions. In a field experiment on a Danish sandy loam soil we compared N2O emissions during winter barley growth following five years of direct drilling (DD), reduced tillage (RT) or conventional tillage (CT). Each of these tillage treatments further varied in respect to whether the resulting plot crop residues were retained (+Res) or removed (−Res). Sampling took place from autumn 2007 to the end of spring 2008. Overall N2O emissions were 27 and 26% lower in DD and RT, respectively, relative to N2O emissions from CT plots (P < 0.05). We observed that in residue removal scenarios N2O emissions were similar for all tillage treatments, but in residue retention scenarios N2O emissions were significantly higher in CT than in either DD or RT (P < 0.05). Irrespective of residue management, N2O emissions from DD and RT plots never exceeded emissions from CT plots. Retention of residue was estimated to reduce emissions from DD plots by 39% and in RT plots by 9%, but to increase N2O emissions from the CT plots by 35%. Relative soil gas diffusivity (Rdiff), soil NO3-N, soil temperature, tillage and residue were important driving factors for N2O emission (P < 0.05). A multiple linear regression model using Rdiff to represent the water factor explained N2O emissions better than a water-filled pore space (WFPS) based model, suggesting a need for review of the current use of WFPS in N2O prediction models. We conclude that on light textured soils, no-till has the potential for reducing N2O emissions when crop residues are returned to the soil.  相似文献   

9.
Organic matter influences soil structure and compactibility by binding soil mineral particles, reducing aggregate wettability, and influencing the mechanical strength of soil aggregates, which is the measure of coherence of inter-particle bonds. This work was carried out to examine how differences in water-stable aggregates influence the distribution of soil organic carbon and soil organic nitrogen under two tillage techniques [minimum tillage (only planting holes were opened) and conventional tillage (raised beds, 30 cm high, prepared manually with traditional hoes)] in soils of a Fluvisol in Owerri, southeastern Nigeria. Three pedons were dug and studied for each of the tillage technique along a soil sequence. Soil organic carbon and soil organic nitrogen distribution in whole soil and in water-stable aggregates under minimum tillage and conventional tillage were determined for the soils. Soil organic carbon contents in water-stable aggregates (WSA) of the pedons varied according to method of tillage. The highest mean values of soil organic carbon were obtained from minimum tillage and in water-stable aggregates 4.75–2.00 mm (16.03 Mg C ha−1), 1.00–0.50 mm (14.06 Mg C ha−1) and water-stable aggregates 2.00–1.00 mm (13.99 Mg C ha−1) whereas under conventional tillage, water-stable aggregates 1.00–0.50 mm with soil organic carbon of 24.6 Mg C ha−1 had the highest soil organic carbon content. Soil organic carbon correlated significantly with mean weight diameter (r = 0.48; P = 0.05; n = 15), water-stable aggregates 4.75–2.00 mm (r = 0.73; P = 0.05; n = 15), water-stable aggregates 2.00–1.00 mm (r = 0.55; P = 0.05, n = 15), water-stable aggregates 1.00–0.50 mm (r = 0.44; P = 0.05; n = 15) whereas no relationship was found between soil organic carbon and water-stable aggregates 0.50–0.25 mm (r = 0.15; P = 0.05; n = 15) and water-stable aggregates <0.25 mm (r = 0.17; P = 0.05; n = 15) in soils under minimum tillage. There was a significant correlation (r = 0.45–0.58; P = 0.05; n = 14) between all water-stable aggregates classes studied and soil organic carbon in soils under conventional tillage. Mean values of soil organic nitrogen were higher in soils under minimum tillage with 4.75–2.00 mm and 2.00–1.00 mm aggregate classes having 1.64 Mg N ha−1 and 1.57 Mg N ha−1 soil organic nitrogen when compared to 1.01 Mg N ha−1 and 1.00 Mg N ha−1 in conventionally tilled soils of the same aggregate classes, respectively. Larger water-stable aggregate classes (4.75–2.00; 2.00–1.00) had slightly more soil organic nitrogen (22–26%) than smaller aggregate classes (1.00–0.50; 0.50–0.25; >0.25) with 14–24% soil organic nitrogen in minimum tilled soils. In soils under conventional tillage, 1.00–0.50 mm, 0.50–0.25 mm and <0.25 mm aggregate classes contributed more soil organic nitrogen (19.66–22.40%) to the soil whereas larger water-stable aggregate classes contributed 19.22% soil organic nitrogen. The proportion of soil organic carbon and total nitrogen retained in soils with higher percentage of water-stable aggregates are less likely to be lost through soil and wind erosion. The higher values of SOC in the whole soil and WSA classes less than 2.00 mm are indications of positive influence of SOC on the stability of these peds.  相似文献   

10.
Emission of N2O and CH4 oxidation rates were measured from soils of contrasting (30-75%) water-filled pore space (WFPS). Oxidation rates of 13C-CH4 were determined after application of 10 μl 13C-CH4 l−1 (10 at. % excess 13C) to soil headspace and comparisons made with estimates from changes in net CH4 emission in these treatments and under ambient CH4 where no 13C-CH4 had been applied. We found a significant effect of soil WFPS on 13C-CH4 oxidation rates and evidence for oxidation of 2.2 μg 13C-CH4 d−1 occurring in the 75% WFPS soil, which may have been either aerobic oxidation occurring in aerobic microsites in this soil or anaerobic CH4 oxidation. The lowest 13C-CH4 oxidation rate was measured in the 30% WFPS soil and was attributed to inhibition of methanotroph activity in this dry soil. However, oxidation was lowest in the wetter soils when estimated from changes in concentration of 12+13C-CH4. Thus, both methanogenesis and CH4 oxidation may have been occurring simultaneously in these wet soils, indicating the advantage of using a stable isotope approach to determine oxidation rates. Application of 13C-CH4 at 10 μl 13C-CH4 l−1 resulted in more rapid oxidation than under ambient CH4 conditions, suggesting CH4 oxidation in this soil was substrate limited, particularly in the wetter soils. Application of and (80 mg N kg soil−1; 9.9 at.% excess 15N) to different replicates enabled determination of the respective contributions of nitrification and denitrification to N2O emissions. The highest N2O emission (119 μg 14+15N-N2O kg soil−1 over 72 h) was measured from the 75% WFPS soil and was mostly produced during denitrification (18.1 μg 15N-N2O kg soil−1; 90% of 15N-N2O from this treatment). Strong negative correlations between 14+15N-N2O emissions, denitrified 15N-N2O emissions and 13C-CH4 concentrations (r=−0.93 to −0.95, N2O; r=−0.87 to −0.95, denitrified 15N-N2O; P<0.05) suggest a close relationship between CH4 oxidation and denitrification in our soil, the nature of which requires further investigation.  相似文献   

11.
Nitrous oxide (N2O) and methane (CH4) emitted by anthropogenic activities have been linked to the observed and predicted climate change. Conservation tillage practices such as no-tillage (NT) have potential to increase C sequestration in agricultural soils but patterns of N2O and CH4 emissions associated with NT practices are variable. Thus, the objective of this study was to evaluate the effects of tillage practices on N2O and CH4 emissions in long-term continuous corn (Zea mays) plots. The study was conducted on continuous corn experimental plots established in 1962 on a Crosby silt loam (fine, mixed, mesic Aeric Ochraqualf) in Ohio. The experimental design consisted of NT, chisel till (CT) and moldboard plow till (MT) treatments arranged in a randomized block design with four replications. The N2O and CH4 fluxes were measured for 1-year at 2-week intervals during growing season and at 4-week intervals during the off season. Long-term NT practice significantly decreased soil bulk density (ρb) and increased total N concentration of the 0–15 cm layer compared to MT and CT. Generally, NT treatment contained higher soil moisture contents and lower soil temperatures in the surface soil than CT and MT during summer, spring and autumn. Average daily fluxes and annual N2O emissions were more in MT (0.67 mg m−2 d−1 and 1.82 kg N ha−1 year−1) and CT (0.74 mg m−2 d−1 and 1.96 kg N ha−1 year−1) than NT (0.29 mg m−2 d−1 and 0.94 kg N ha−1 year−1). On average, NT was a sink for CH4, oxidizing 0.32 kg CH4-C ha−1 year−1, while MT and CT were sources of CH4 emitting 2.76 and 2.27 kg CH4-C ha−1 year−1, respectively. Lower N2O emission and increased CH4 oxidation in the NT practice are attributed to decrease in surface ρb, suggesting increased gaseous exchange. The N2O flux was strongly correlated with precipitation, air and soil temperatures, but not with gravimetric moisture content. Data from this study suggested that adoption of long-term NT under continuous corn cropping system in the U.S. Corn Belt region may reduce GWP associated with N2O and CH4 emissions by approximately 50% compared to MT and CT management.  相似文献   

12.
This study evaluated carbon and nutrient distributions within water-stable aggregates (WSA) of soils of two contrasting ecosystems under different land uses. Surface soil samples were collected from uncultivated and cultivated land in rainforest and savannah agro-ecological areas and separated by wet-sieving technique into 4.76–2.0, 2.0–1.0, 1.0–0.50, 0.50–0.25 and <0.25 mm aggregate fractions. The results show that irrespective of the agro-ecological area, cultivation significantly (p < 0.05) reduced the macroaggregate fractions (>0.25 mm) to smaller diameters. Distribution of organic carbon (C), total nitrogen (N) and available phosphorus (P) within the WSA showed preferential enrichment of these elements in the large macroaggregate fraction (4.76–2.0 mm) for the uncultivated soils and microaggregate fraction (<0.25 mm) for the cultivated soils. The overall pattern indicates higher accumulation of C, N and P in the WSA of the uncultivated soils over the cultivated soils. Average distribution of total exchangeable bases (TEB), i.e., sum of Ca2+, Mg2+, K+ and Na+, within WSA of the uncultivated soils of the rainforest region were 7.35 and 7.39 cmol/kg for 4.76–2.0 and <0.25 mm fractions, respectively. The distributions of TEB for cultivated soils of the rainforest region were 2.76 cmol/kg (4.76–2.0 mm fraction) and 7.73 cmol/kg for <0.25 mm fraction. This showed that cultivation significantly (p < 0.05) led to 62% reduction in these nutrients in the 4.76–2.0 mm fraction and 5% increase in concentrations of these cations in <0.25 mm fraction. For savannah soils, distributions of TEB were 7.44 and 6.77 cmol/kg for 4.76–2.0 and <0.25 mm fractions, respectively, in uncultivated sites, whereas TEB were 2.19 cmol/kg (4.76–2.0 mm) and 6.35 cmol/kg (<0.25 mm) for cultivated savannah. This indicated that cultivation significantly (p < 0.05) led to 71% and 6% reductions in Ca2+, Mg2+, K+ and Na+ concentrations within the 4.76–2.0 and <0.25 mm aggregate fractions, respectively. However, there were 18% and 50% increase in these elements in the 2.0–1.0 and 1.0–0.50 mm fractions of the cultivated soils of the savannah region, respectively. The general trend showed that in uncultivated soils, the 4.76–2.0 and <0.25 mm fractions were preferentially enriched with Ca2+, Mg2+, K+ and Na +; whereas, cultivation led to redistribution of these elements into the smaller aggregates. Since smaller aggregates are preferentially removed by erosion, this study underscores the need for sustainable soil management practices that would minimize nutrient loss when forest or fallow lands are converted to cropland.  相似文献   

13.
Soil organic matter improves the physical, chemical and biological properties of soil, and crop residue recycling is an important factor influencing soil organic matter levels. We studied the impact of continuous application of rice straw compost either alone or in conjunction with inorganic fertilizers on aggregate stability and distribution of carbon (C) and nitrogen (N) in different aggregate fractions after 10 cycles of rice–wheat cropping on a sandy loam soil at Punjab Agricultural University research farm, Ludhiana, India. Changes in water stable aggregates (WSA), mean weight diameter (MWD), aggregate-associated C and N, total soil C and N, relative to control and inorganically fertilized soil were measured. Total WSA were significantly (p = 0.05) higher for soils when rice straw compost either alone or in combination with inorganic fertilizers was applied as compared to control. The application of rice straw compost either alone or in combination with inorganic fertilizers increased the macroaggregate size fractions except for 0.25–0.50 mm fraction. The MWD was significantly (p = 0.05) higher in plots receiving rice straw compost either alone at 8 tonnes ha−1 (0.51 mm at wheat harvest and 0.41 mm at rice harvest) or at 2 tonnes ha−1 in combination with inorganic fertilizers (0.43 and 0.38 mm) as compared to control (0.34 and 0.33 mm) or inorganically fertilized plots (0.33 and 0.31 mm). The macroaggregates had higher C and N density compared to microaggregates. Application of rice straw compost at 2 tonnes ha−1 along with inorganic fertilizers (IN + 2RSC) increased C and N concentration significantly over control. The C and N concentration increased further when rice straw compost at 8 tonnes ha−1 (8RSC) was added. It is concluded that soils can be rehabilitated and can sustain the soil C and N levels with the continuous application of rice straw compost either alone or in combination with inorganic fertilizers. This will also help in controlling the rising levels of atmospheric carbon dioxide.  相似文献   

14.
It has been well documented that restored wetlands in the Prairie Pothole Region of North America do store carbon. However, the net benefit of carbon sequestration in wetlands in terms of a reduction in global warming forcing has often been questioned because of potentially greater emissions of greenhouse gases (GHGs) such as nitrous oxide (N2O) and methane (CH4). We compared gas emissions (N2O, CH4, carbon dioxide [CO2]) and soil moisture and temperature from eight cropland and eight restored grassland wetlands in the Prairie Pothole Region from May to October, 2003, to better understand the atmospheric carbon mitigation potential of restored wetlands. Results show that carbon dioxide contributed the most (90%) to net-GHG flux, followed by CH4 (9%) and N2O (1%). Fluxes of N2O, CH4, CO2, and their combined global warming potential (CO2 equivalents) did not significantly differ between cropland and grassland wetlands. The seasonal pattern in flux was similar in cropland and grassland wetlands with peak emissions of N2O and CH4 occurring when soil water-filled pore space (WFPS) was 40-60% and >60%, respectively; negative CH4 fluxes were observed when WFPS approached 40%. Negative CH4 fluxes from grassland wetlands occurred earlier in the season and were more pronounced than those from cropland sites because WFPS declined more rapidly in grassland wetlands; this decline was likely due to higher infiltration and evapotranspiration rates associated with grasslands. Our results suggest that restoring cropland wetlands does not result in greater emissions of N2O and CH4, and therefore would not offset potential soil carbon sequestration. These findings, however, are limited to a small sample of seasonal wetlands with relatively short hydroperiods. A more comprehensive assessment of the GHG mitigation potential of restored wetlands should include a diversity of wetland types and land-use practices and consider the impact of variable climatic cycles that affect wetland hydrology.  相似文献   

15.
Agricultural intensification has led to the use of very high inputs of nitrogen fertilizers into cultivated land. As a consequence of this, nitrous oxide (N2O) emissions have increased significantly. Nowadays, the challenge is to mitigate these emissions in order to reduce global warming. Addition of nitrification inhibitors (NI) to fertilizers can reduce the losses of N2O to the atmosphere, but field studies have shown that their efficiency varies depending greatly on the environmental conditions. Soil water content and temperature are key factors controlling N2O emissions from soils and they seem to be also key parameters responsible for the variation in nitrification inhibitors efficiency. We present a laboratory study aimed at evaluating the effectiveness of the nitrification inhibitor 3,4-dimethylpyrazol phosphate (DMPP) at three different temperatures (10, 15 and 20 °C) and three soil water contents (40%, 60% and 80% of WFPS) on N2O emissions following the application of 1.2 mg N kg−1 dry soil (equivalent to 140 kg N ha−1). Also the CO2 and CH4 emissions were followed to see the possible side effects of DMPP on the overall microbial activities. Nitrogen was applied either as ammonium sulfate nitrate (ASN) or as ENTEC 26 (ASN + DMPP). The application of ENTEC 26 was effective reducing N2O losses up to the levels of an unfertilized control treatment in all conditions. Nevertheless, the percentage of reduction induced by DMPP in the ENTEC treatment with respect to the ASN varied from 3% to 45% depending on temperature and soil water content conditions. At 40% of WFPS, when nitrification is expected to be the main process producing N2O, the increase of N2O emissions in ASN together with temperature provoked an increase in DMPP efficiency reducing these emissions from 17% up to 42%. Contrarily, at 80% of WFPS, when denitrification is expected to be the main source of N2O, emissions after ASN application decreased with temperature, which induced a decrease from 45% to 23% in the efficiency of DMPP reducing N2O losses. Overall, the results obtained in this study suggest that DMPP performance regarding N2O emissions reduction would be the best in cold and wet conditions. Neither CO2 emissions nor CH4 emissions were affected by the use of DMPP at the different soil water contents and temperatures.  相似文献   

16.
硝化反应是土壤、特别是干旱半干旱地区农业土壤N2O产生的重要途径之一。但是,目前环境条件对硝化反应中N2O排放的影响研究较少,而在国内外通用的几个模型中均用固定比例估算硝化反应过程中N2O的排放。本文通过砂壤土培养试验,研究了土壤温度、水分和NH4+-N浓度对硝化反应速度及硝化反应中N2O排放的影响,并用数学模型定量表示了各因素对硝化反应的作用,用最小二乘法最优拟合求得该土壤的最大硝化反应速度及N2O最大排放比例。结果表明,随着温度升高,硝化反应速度呈指数增长;水分含量由20%充水孔隙度(WFPS)增加到40%WFPS时,反应速度增加,水分含量增加到60%WFPS时反应速度略有降低;NH4+-N浓度增加对硝化反应速度起抑制作用。用米氏方程描述该土壤的硝化反应过程,其最大硝化反应速度为6.67mg·kg?1·d?1。硝化反应中N2O排放比例随温度升高而降低;随NH4+-N浓度增加而略有增加;20%和40%WFPS水分含量时,硝化反应中N2O排放比例为0.43%～1.50%,最小二乘法求得的最大比例为3.03%,60%WFPS时可能由于反硝化作用,N2O排放比例急剧增加,还需进一步研究水分对硝化反应中N2O排放的影响。  相似文献   

17.
Farmyard manure (FYM) and fertilizer applications are important management practices used to improve nutrient status and organic matter in soils and thus to increase crop productivity and carbon (C) sequestration. However, the long-term effects of fertilization on C, nitrogen (N) and sulfur (S) associated with aggregates, especially on S are not fully understood. We investigated the effects of more than 80 years of FYM (medium level of 40 Mg ka−1 and high level of 60 Mg ka−1) and mineral fertilizer (NPKS and NK) on the concentrations and pools of C, N, and S and on their ratios in bulk soil, dry aggregates and water stable aggregates on an Aquic Eutrocryepts soil in South-eastern Norway. A high level of FYM and NPKS application increased the proportion of small dry aggregates (<0.6 mm) by 8%, compared with the control (without fertilizer). However, both medium and high level of FYM application increased the proportion of large water stable aggregates (>2 mm) compared with mineral fertilizer (NPKS and NK). The total C and N pools in bulk soils were also increased in FYM treatments but no such increase was seen with mineral fertilizer treatments. The increased total S pool was only found under high level of FYM application. Water stable macroaggregates (>2 and 1–2 mm) and microaggregates (<0.106 mm) contained higher concentrations of C, N and S than the other aggregate sizes, but due to their abundance, medium size water stable aggregates (0.5–1 mm) contained higher total pools of all three elements. High level of FYM application increased the C concentration in water stable aggregates >2, 0.5–1 and <0.106 mm, and increased the S concentration in most aggregates as compared with unfertilized soils. Higher C/N, C/S and N/S ratios were found both in large dry aggregates (>20 and 6–20 mm) and in the smallest aggregates (<0.6 mm) than in other aggregate sizes. In water stable aggregates, the C/N ratio generally increased with decreasing aggregate size. However, macroaggregates (>2 mm) showed higher N/S ratios than microaggregates (<0.106 mm). We can thus conclude, that long-term application of high amounts of FYM resulted in C, N and S accumulation in bulk soil, and C and S accumulation in most aggregates, but that the accumulation pattern was dependent on aggregate size and the element (C, N and S) considered.  相似文献   

18.
氧化亚氮（N2O）是主要温室气体之一,土壤是N2O的重要排放源,其排放主要受N2O产生和还原的功能微生物影响。土壤团聚体是由原生颗粒（砂、粉、黏粒）、胶结物质和孔隙组成的土壤基本结构单元。土壤不同粒径团聚体之间因基质和孔隙差异形成特殊独立的微生境被视为N2O的生物化学反应器。在不同的微生境中,N2O产生和还原的功能微生物分布不同,因而土壤不同粒径团聚体N2O排放可能存在差异。目前在不同生态系统土壤全土N2O排放特征的报道较多,而对于不同粒径土壤团聚体N2O排放相对贡献尚不清楚、功能微生物分布还未知、N2O产生和还原热区尚未明确。本文综述了近年来国内外关于土壤团聚体对N2O产生和排放机制的研究,总结了土壤团聚体性状特征对N2O产生和还原的影响,阐述了不同粒径土壤团聚体对N2O排放影响的微生物学机制,进一步明确了今后需加强土壤团聚体N2O产生和还原的热区、环境因子阈值范围的确定、系列功能基因（酶）整体性的研究,以期为N2O模拟排放模型优化提供参考,为土壤N2O减排提供理论依据。  相似文献   

19.
ABSTRACT

Antecedent soil moisture before freezing can affect greenhouse gases (GHG) fluxes from soils during thaw, but their critical threshold values for GHG fluxes and the underlying mechanisms are still not clear. By using packed soil-core incubation experiments, we have studied nitrous oxide (N2O), carbon dioxide (CO2) and methane (CH4) fluxes from a mature broadleaf and Korean pine-mixed forest soil and an adjacent white birch forest soil with nine levels of soil moisture ranging from 10 to 90% water-filled pore space (WFPS) during a 2-month freezing at ?8°C and the following 10-day thaw at 10°C. The threshold values of soil moisture ranged from 50 to 70% WFPS for CH4 uptake and from 70 to 90% WFPS for N2O and CO2 emissions from the two soils during the freeze-thaw period. Under the optimum soil moisture condition, fulvic-like compounds with high bioavailability contributed more than 60% of dissolved organic matter (DOM) in the soil. Cumulative N2O emissions from forest soils during the freeze-thaw period were greatest when the concentration ratio of nitrate-N to dissolved organic carbon (DOC) was 0.04 g N g?1 C. Cumulative soil CO2 emissions and CH4 uptake during the freeze-thaw period were both regulated by the interaction between soil DOC and net N mineralization. The activities of β-1,4-glucosidase and β-1,4-N-acetyl-glucosaminidase, microbial biomass C and N, and the microbial biomass C-to-N ratios, were all significantly correlated to the soil N2O, CO2, and CH4 fluxes. Overall, upon a freeze-thaw period with different soil moistures, GHG fluxes from forest soils were jointly regulated by inorganic N and DOC concentrations, and related to the labile components of DOM released into the soil, which could be strictly controlled by the related microbial properties.  相似文献   

20.
Knowledge of the cycling and compartmentalization of soil C that influence C storage may lead to the development of strategies to increase soil C storage potentials. The objective of this study was to use soil hydrolases and soil aggregate fractionation to explore the relationship between C cycling activity and soil aggregate structure. The prairie chronosequence soils were native prairie (NP) and agricultural (AG) and tallgrass prairies restored from agriculture in 1979 (RP-79) and 1993 (RP-93). Assays for -glucosidase (E.C. 3.2.1.21) and N-acetyl--glucosaminidase (NAGase, EC 3.2.1.30) activities were conducted on four aggregate size fractions (>2 mm, 1–2 mm, 250 m–1 mm, and 2–250 m) from each soil. There were significantly greater amounts of >2-mm aggregates in the RP-79 and RP-93 soils compared to the NP and AG soils due to rapid C accumulation from native plant establishment. Activities for both enzymes (g PNP g–1 soil h–1) were greatest in the microaggregate (2–250 m) compared to the macroaggregate (>2 mm) fraction; however, microaggregates are a small proportion of each soil (<12%) compared to the macroaggregates (75%). The RP soils have a hierarchical aggregate system with most of the enzyme activity in the largest aggregate fractions. The NP and AG soils show no hierarchical structure based on aggregate C accretion and significant C enzyme activity in smaller aggregates. The distribution of enzyme activity may play a role in the storage of C whereby the aggrading restored soils may be more susceptible to C loss during turnover of macroaggregates compared to the AG and NP soils with less macroaggregates.  相似文献   

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