共查询到18条相似文献，搜索用时 250 毫秒
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茧壳水煎剂对四氧嘧啶糖尿病模型小鼠的降血糖作用 总被引：2，自引：0，他引：2
用不同剂量的茧壳水煎剂分别对正常小鼠和四氧嘧啶糖尿病模型小鼠进行了降血糖作用研究。结果显示 :干茧壳剂量为 1 6、3 2g/kg·d的茧壳水煎剂对正常小鼠连续灌胃 2 0d ,其血糖水平没有明显变化 ;用干茧壳剂量为 1 6g/kg·d的茧壳水煎剂对四氧嘧啶糖尿病模型小鼠连续灌胃 2 0d ,或用干茧壳剂量为 3 2g/kg·d的茧壳水煎剂对四氧嘧啶糖尿病模型小鼠连续灌胃 10d ,其血糖没有显著降低 ;而干茧壳剂量为 3 2g/kg·d的茧壳水煎剂对四氧嘧啶糖尿病模型小鼠连续灌胃 14、2 0d ,有显著的降血糖作用 ,但是若不继续灌胃给药 ,则 7d后 ,这种降血糖作用又丧失。该研究结果为利用蚕茧治疗糖尿病提供了药理学方面的依据 相似文献
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柞树叶水提物对Ⅱ型糖尿病模型小鼠的治疗作用 总被引：1，自引：0，他引：1
《蚕业科学》2017,(4)
采用高糖高脂膳食加链脲佐菌素诱导制作Ⅱ型糖尿病模型小鼠,研究柞树叶水提物调节糖尿病模型小鼠血糖浓度、改善生命体征和减轻脏器损伤的作用。与模型对照组相比,按照5、15、30 mg/(kg·d)3种剂量灌胃给予柞树叶水提物的糖尿病模型小鼠的体征均有所改善,饮水和摄食量减少,且呈剂量依赖性;试验第4周,3种剂量给药治疗组糖尿病模型小鼠的空腹血糖浓度均显著或极显著降低,胰岛素敏感指数(ISI)显著升高,血清胰岛素(INS)和胰岛素受体β(IRS-β)浓度未见明显变化;3种剂量给药治疗组小鼠肝组织病理变化程度明显减轻,以30 mg/(kg·d)剂量给药治疗组效果最佳。试验结果提示,柞树叶水提物可能通过提高受体对胰岛素的敏感性,改善胰岛素抵抗而发挥降血糖作用,并使肝损伤明显减轻。 相似文献
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富铬酵母对试验性糖尿病小鼠糖代谢酶活性的影响 总被引：1，自引：0，他引：1
为了探讨富铬酵母对链脲佐菌素造模糖尿病小鼠糖代谢及关键酶己糖激酶、苹果酸脱氢酶活性的影响,探讨有机铬对糖尿病作用的机理和有效剂量,试验用腹腔注射链脲佐菌素(STZ)的方法建立小鼠糖尿病模型,分别以高、中、低剂量富铬酵母和西药二甲双胍进行干预,试验期为4周,每周末尾静脉检测空腹血糖值,试验结束时检测血糖值、己糖激酶(HK)、苹果酸脱氢酶活性(MDH).结果表明:富铬酵母在125 mg/kg剂量时显示降糖效果,在中、高剂量时与模型组相比有显著性差异,富铬酵母剂量越高,对葡萄糖耐受能力越强.用药组肌肉组织己糖激酶、肝脏组织苹果酸脱氢酶活性与模型组相比有显著差异(P<0.05).糖尿病小鼠血糖水平与己糖激酶、苹果酸脱氢酶活性呈显著的负相关(P<0.05).说明富铬酵母降低血糖的作用机制之一可能是直接或间接提高了葡萄糖氧化分解酶的活性. 相似文献
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桑叶粉对肉鸡生长性能和屠宰性能及肉质的影响 总被引：1，自引：0，他引：1
本试验旨在研究桑叶粉对肉鸡生长性能、屠宰性能及肉质的影响。选择35日龄的广西青脚麻公鸡172只,随机分为4组,分别饲以桑叶粉添加量为0(对照组)、5%、8%和11%的饲粮。结果表明:8%、11%桑叶粉组的饲料增重比相对于对照组显著提高(P<0.05);5%桑叶粉组的肉鸡腹脂率显著降低(P<0.05);与对照组相比,桑叶粉组的肌内脂肪(IMF)含量显著降低(P<0.05);11%桑叶粉组的不饱和脂肪酸(UFA)、ω-3脂肪酸含量显著提高(P<0.05),饱和脂肪酸(SFA)含量显著降低(P<0.05);桑叶粉组的矫正肌苷酸含量有上升趋势,但差异不显著(P>0.05)。综上,桑叶粉的添加具有降低腹脂率、IMF和提高UFA、ω-3脂肪酸含量的效果,但使肉鸡的饲料利用率降低。 相似文献
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抗病毒合剂的抗炎作用研究 总被引：2，自引：2，他引：0
抗病毒合剂由具有抗病毒、清热、滋阴凉血等作用的中草药经水煎醇沉、浓缩制备而成 ,以小鼠为试验动物模型 ,选择不同剂量 (0 .3ml、0 .5 ml、0 .8m l)研究了该合剂的抗炎作用。结果 :0 .5 m l和 0 .8m l剂量对小鼠腹腔毛细血管通透性、小鼠皮肤毛细血管通透性、二甲苯所致小鼠耳壳肿胀及小鼠棉球肉芽肿均有极显著的抑制作用 (P<0 .0 1) ,0 .3m l剂量对前三个试验小鼠也有显著的抑制作用 (P<0 .0 5 )。结论 :该合剂有很强的抗炎作用 ,而且与剂量呈正相关性。 相似文献
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为了研究体重与剂量对链脲佐菌素致糖尿病小鼠模型的影响,采用不同体重范围的雄性小鼠,对其腹腔注射不同剂量的链脲佐菌素,剂量范围为:100～300mg/(kg·bw),分5次注射造模。在不同时间测量试验鼠的血糖值、死亡率及成模率,观察小鼠的糖尿病发展病程,确定链脲佐菌素最佳用药剂量。结果表明,当给雄性小鼠腹腔注射200mg/(kg·bw)的链脲佐菌素后,造模成功率最高为85%,小鼠表现出典型的糖尿病症状,且成模饲养1周后血糖、体重变化不大,为制备糖尿病小鼠模型的最佳剂量;同等剂量的情况下,低体重小鼠比高体重小鼠的成模率高、死亡率低。 相似文献
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多化性黄血蚕的食用和药用价值的研究 总被引：18，自引：8，他引：10
黄血蚕含有 6 0 %的蛋白质 ,2 1%的脂肪 ,其中不饱和脂肪酸占油脂总量的 93% ,亚油酸、α 亚麻酸含量达 13 4 9%及 30 95 % ,此外还含有较高的维生素A。实验表明 :黄血蚕降脂Ⅰ号表现极显著降低高血脂模型小鼠血清总胆固醇 (TC)和血清甘油三脂 (TG)的效果 (P <0 0 1) ,并在一定剂量范围内显示出明显的剂量效应关系 ;金蚕宝胶囊可显著改善四氧嘧啶诱导的糖尿病小鼠三多一少的症状及降低血糖值 ,其中饲料消耗量、饮水量和尿量分别降低 2 5 %、4 7%和 4 3% ,并能维持体重 ,血糖值在对照组上升 5 6 %的状况下降低 33% (P <0 0 1)。由此认为黄血蚕有较高的食用和药用开发价值 相似文献
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饲料中添加桑叶粉对单胃哺乳动物小鼠的脂肪代谢影响 总被引：1，自引：0，他引：1
以幼年及不同性别成年小鼠为动物模型,探讨桑叶作为动物饲料对单胃动物脂肪代谢的影响。在主要营养水平基本一致的条件下,给不同类型模型小鼠分别饲喂含20%和40%桑叶粉的饲料,幼年小鼠连续饲喂21 d、成年小鼠连续饲喂15 d,分别取血清、小肠、胰腺、腹部脂肪、肌肉等组织器官做检测。测定小鼠脂类代谢指标的结果表明,饲喂添加桑叶粉的饲料可不同程度降低小鼠血清甘油三酯含量,并极显著降低小鼠总胆固醇含量、腹脂系数和雌性小鼠肌肉脂肪含量(P<0.01),但可极显著促进雄性小鼠肌肉脂肪沉积(P<0.01);测定小鼠营养物质代谢关键酶活性的结果表明,饲喂添加桑叶粉的饲料可不同程度提高幼年小鼠血清中的淀粉酶活性,但会极显著抑制小肠中的蔗糖酶、麦芽糖酶以及血清中的脂肪酶活性(P<0.01),而胰腺脂肪酶活性则不同程度升高。另对小鼠的生长发育调查表明,饲喂含20%桑叶粉的饲料,不影响幼年小鼠正常生长发育,但可减缓成年小鼠体质量增长速度。推测当饲料中的桑叶粉质量分数≥20%时,桑叶粉中的活性物质可调节哺乳动物体内碳水化合物和脂肪代谢关键酶的活性,从而控制体内的脂肪沉积,这为生产低脂高品质畜禽产品以及拓宽桑叶新用途提供了新思路。 相似文献
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通过体外α-葡萄糖苷酶抑制试验和糖尿病模型小鼠动物试验,检验桑树内生菌菌株Stenotrophomonas maltophilia Y1和Micrococcus luteus Y2发酵产物的降血糖作用。结果表明,Y1和Y2菌株发酵液提取物对α-葡萄糖苷酶均具有显著的体外抑制作用,并呈现一定的剂量依赖性,半数抑制浓度(IC50)分别为2 307.56 mg/L和111.58 mg/L,在试验最大剂量下的抑制率分别达到59.17%和69.21%,均高于对照药物拜糖平(抑制率为53.06%)。动物试验表明,饲喂Y1菌株发酵液浓缩产物的糖尿病模型小鼠的空腹血糖(FBG)值和餐后血糖(PBG)值均低于模型组,分别存在显著和极显著差异,而对正常小鼠的血糖无明显影响,也未表现出毒副作用;调查饲喂发酵产物后糖尿病模型小鼠的体质量、进食量、饮水量、排尿量等指标,均得到明显改善。试验结果显示,2株桑树内生菌菌株的发酵液提取物均表现出与1-脱氧野尻霉素(DNJ)相同的体外抑制α-葡萄糖苷酶活性的作用,Y1菌株发酵产物表现出与DNJ相同的降低糖尿病模型小鼠血糖及改善糖尿病症状的作用特点。 相似文献
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桑叶多糖MLPⅡ的基本结构及对糖尿病模型大鼠的降血糖作用 总被引：2，自引：2，他引：0
从桑叶中分离纯化获得一种具有降血糖活性的均一多糖组分MLPⅡ。高效液相色谱分析桑叶多糖MLPⅡ主要由甘露糖(man)、鼠李糖(rha)、葡萄糖(glc)、木糖(xyl)和阿拉伯糖(ara)等5种单糖组成,其摩尔比为n(man)∶n(rha)∶n(glc)∶n(xyl)∶n(ara)=8.73∶1.04∶6.53∶2.13∶1.00;红外光谱显示桑叶多糖MLPⅡ的主要连接键为β-糖苷键。桑叶多糖MLPⅡ对糖尿病模型大鼠的降血糖试验结果表明,150 mg/kg MLPⅡ治疗组糖尿病模型大鼠的体质量与对照组糖尿病模型大鼠相比提高了26.22%,而空腹血糖(FBG)浓度降低了52.89%,达到极显著水平,并且治疗组糖尿病模型大鼠的糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)和C-肽(C-P)水平得到改善,损伤胰岛细胞得到修复,胰岛β细胞合成和胰岛素分泌的能力增强。研究结果提示,桑叶多糖MLPⅡ可有效控制糖尿病模型大鼠的体质量和空腹血糖水平,并改善糖耐量,而促进糖尿病模型大鼠胰岛β细胞合成及胰岛素分泌可能是桑叶多糖MLPⅡ降血糖作用机制之一。 相似文献
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柞蚕全蚕粉对诱导型小鼠糖尿病的预防作用 总被引：2，自引：0，他引：2
饲喂添加不同剂量柞蚕全蚕粉的全价配合饲料4周后,通过尾静脉注射四氧嘧啶建立糖尿病小鼠模型,观察柞蚕全蚕粉对糖尿病小鼠血糖、脂代谢[甘油三酯(TG),总胆固醇(TC),高密度脂蛋白(HDL-C),低密度脂蛋白(LDL-C)]的影响。结果表明:添食中、高剂量柞蚕全蚕粉可显著降低糖尿病小鼠血糖,分别比阳性对照组降低了31.3%、42.5%(p<0.01)。各剂量组TG、TC、LDL-C较阳性对照组均有不同程度降低,其中:高剂量组LDL-C与阴性对照组无差异(p>0.05);HDL-C较阳性对照组均有升高,中、高剂量组与阳性对照组有显著性差异(p<0.05)。柞蚕全蚕粉能够降低糖尿病小鼠的血糖,且调节糖尿病小鼠的脂代谢和降低TG,TC。 相似文献
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Nelson RW Lynn RC Wagner-Mann CC Michels GM 《Journal of the American Veterinary Medical Association》2001,218(1):38-42
OBJECTIVE: To evaluate effects of protamine zinc insulin (PZI) on control of glycemia in cats with newly diagnosed diabetes mellitus or poorly controlled diabetes. DESIGN: Clinical trial. ANIMALS: 67 diabetic cats. PROCEDURE: 34 cats with newly diagnosed diabetes and 33 cats with poorly controlled diabetes were treated with PZI twice daily for 45 days. Control of glycemia was assessed on days 7, 14, 30, and 45 by evaluation of clinical response, change in body weight, serum fructosamine concentration, blood glucose concentration measured 1, 3, 5, 7, and 9 hours after administration of PZI, lowest blood glucose concentration, and mean blood glucose concentration during the 9-hour period after administration. Adjustments in dosage of PZI were made as needed to attain control of glycemia. RESULTS: For all cats, a significant increase in mean dosage of PZI and significant decreases in 9-hour mean blood glucose concentration, lowest mean blood glucose concentration, and mean serum fructosamine concentration were detected. For cats with poorly controlled diabetes, 9-hour mean blood glucose concentration and mean serum fructosamine concentration were significantly decreased on day 45, compared with day 0. Ninety percent of owners reported improvement or resolution of clinical signs by day 45. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that PZI was effective for control of glycemia in cats with newly diagnosed or poorly controlled diabetes and may be used as an initial treatment or as an alternative treatment in cats that do not respond to treatment with other types of insulin. 相似文献
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This experiment was conducted to investigate the effect of mulberry leaf meal and fermented mulberry leaf meal on slaughter performance,meat quality and cecum microflora of Huxu chicken at the late growth stage.Three hundred and ninety-two Lingnan Ⅲ Huxu chickens aged ninety-five days were assigned to seven dietary treatments randomly,they were control group,5%,10%,20% mulberry leaf meal groups and 5%,10%,20% fermented mulberry leaf meal groups.Every treatment had 4 replicates,14 chickens per replicates (7 males and 7 females).The pre-trial period was 4 days,and the formal experiment period was 28 days.The results showed as follows:① Compared with the control group,expect the 10% mulberry leaf meal group,the semi-eviscerated percentage were significantly decreased in other experimental groups (P<0.05).Adding 10% mulberry leaf meal or 20% fermenterd mulberry leaf meal extremely significantly reduced the eviscerated percentage (P<0.01) and 10% fermenterd mulberry leaf meal reduced it significantly (P<0.05).The leg muscle percentage of chickens in 5% fermenterd mulberry leaf meal group and 20% mulberry leaf meal group reduced extremely significantly (P<0.01) and significantly (P<0.05),respectively.② Compared with the control group,the shear force of chest muscle in all experimental groups,and leg muscle in all mulberry leaf meal groups decreased,but only the shear force of chest muscle in 10% mulberry leaf meal group decreased significantly (P<0.05).Adding 20% mulberry leaf meal increased the pH45 min of leg muscle significantly (P<0.05).The b* value of leg muscle reduced in all experimental groups,and only that in the 20% mulberry leaf group reduced significantly (P<0.05).③ Compared with the control group,the number of E.coli of the cecum of chickens in 5% and 10% mulberry leaf meal groups reduced extremely significantly (P<0.05),and the ratio of Lactobacillus to E.coli of cecum in all experimental groups had an increasing trend (P>0.05).All over,the content of mulberry leaf meal in Huxu chickens'diet at the late growth stage could be up to 10%,and fermented mulberry leaf meal could be up to 20%. 相似文献
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Nelson RW Robertson J Feldman EC Briggs C 《Journal of the American Veterinary Medical Association》2000,216(8):1265-1269
OBJECTIVE: To evaluate effect of acarbose on control of glycemia in dogs with diabetes mellitus. DESIGN: Prospective randomized crossover controlled trial. ANIMALS: 5 dogs with naturally acquired diabetes mellitus. PROCEDURE: Dogs were treated with acarbose and placebo for 2 months each: in 1 of 2 randomly assigned treatment sequences. Dogs that weighed < or = 10 kg (22 lb; n = 3) or > 10 kg (2) were given 25 or 50 mg of acarbose, respectively, at each meal for 2 weeks, then 50 or 100 mg of acarbose, respectively, at each meal for 6 weeks, with a 1-month interval between treatments. Caloric intake, type of insulin, and frequency of insulin administration were kept constant, and insulin dosage was adjusted as needed to maintain control of glycemia. Serum glucose concentrations, blood glycosylated hemoglobin concentration, and serum fructosamine concentration were determined. RESULTS: Significant differences in mean body weight and daily insulin dosage among dogs treated with acarbose and placebo were not found. Mean preprandial serum glucose concentration, 8-hour mean serum glucose concentration, and blood glycosylated hemoglobin concentration were significantly lower in dogs treated with insulin and acarbose, compared with insulin and placebo. Semisoft to watery feces developed in 3 dogs treated with acarbose. CONCLUSIONS AND CLINICAL RELEVANCE: Acarbose may be useful as an adjunctive treatment in diabetic dogs in which cause for poor glycemic control cannot be identified, and insulin treatment alone is ineffective. 相似文献
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59份野生桑桑叶中的DNJ含量及粗提物对α-糖苷酶的抑制活性 总被引：8，自引：2，他引：6
对来自云南省不同地区的59份野生桑桑叶中的1-脱氧野尻霉素(DNJ)含量,以及桑叶粗提物对淀粉酶、蔗糖酶和麦芽糖酶的体外抑制活性进行了测定,同时采用液-质联用(LC-MS)仪分离纯化桑叶粗提物中的DNJ,并对其抑制蔗糖酶的机制进行了初步研究,期望筛选出适用于治疗糖尿病药物开发的药用桑资源,以及为解析桑叶粗提物和纯化的桑叶DNJ抑制α-糖苷酶活性的机制提供线索。研究结果表明:不同来源的野生桑桑叶中的DNJ含量存在明显差异,其中来自开远地区的岩桑桑叶中DNJ的质量分数高达0.791 1%,是具有药用开发价值的野生桑资源;不同来源的野生桑桑叶粗提物对淀粉酶、蔗糖酶和麦芽糖酶活力的体外抑制率差异显著,但抑制活性的大小与样品中的DNJ含量不成正比,推测桑叶中除含有DNJ外还含有具有明显的α-糖苷酶抑制活性的多种DNJ类似物;从野生桑桑叶粗提物中分离纯化的DNJ对淀粉酶无明显抑制活性,但对蔗糖酶和麦芽糖酶有显著的抑制活性,对蔗糖酶表现为非竞争性抑制作用。 相似文献