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《棉花学报》2021,(2)
【目的】筛选防治烟蓟马(Thrips tabaci Lindeman)的高效生物源农药,探究其对烟蓟马3种主要解毒酶细胞色素P450单加氧酶(Cytochrome P450 monooxygenase,P450)、谷胱甘肽S-转移酶(Glutathione S-transferase,GST)和羧酸酯酶(Carboxylesterase,CarE)活性的影响。【方法】以棉田采集并室内稳定饲养的烟蓟马为供试虫源,用浸叶法测定7种生物源农药对烟蓟马若虫的毒力,选择活性好的3种药剂开展田间药效试验,并在这3种生物源农药亚致死浓度(LC25)剂量处理后测定烟蓟马体内3种主要解毒酶活性。【结果】(1)7种生物源农药对烟蓟马的毒力从高到低依次为乙基多杀菌素、多杀霉素、苦参碱、印楝素、除虫菊素、鱼藤酮、藜芦碱。乙基多杀菌素、多杀霉素和苦参碱对烟蓟马的致死中浓度(LC50)分别为0.011 mg·L-1、0.274 mg·L-1、1.479 mg·L-1。(2)田间药效试验发现,每666.7 m2用2 g、4 g、6 g的60 g·L-1乙基多杀菌素悬浮剂和10 g的5%(质量分数,下同)多杀霉素悬浮剂处理,施药后7 d防治效果均在90%以上,优于1.3%苦参碱水剂和对照药剂30%噻虫嗪悬浮剂各剂量的防效。(3)LC25剂量处理烟蓟马48 h后,乙基多杀菌素处理的烟蓟马体内P450、GST和CarE活性升高,苦参碱和多杀霉素处理后CarE活性升高,噻虫嗪处理后P450和CarE活性升高。【结论】乙基多杀菌素、多杀霉素和苦参碱对烟蓟马的毒力较高,可用于防控棉田烟蓟马;CarE可能参与了烟蓟马对多杀霉素和苦参碱的解毒代谢,3种主要解毒酶可能均参与了烟蓟马对乙基多杀菌素的解毒代谢。 相似文献
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常用生物杀虫剂对3种中草药蚜虫的室内毒力测定 总被引：2，自引：0，他引：2
明确常用生物杀虫剂对中草药蚜虫的室内毒力,为中草药蚜虫的有效防控提供理论依据。用浸渍法测定了4种生物杀虫剂(阿维菌素、印楝素、乙基多杀菌素、苦参碱)对金银花胡萝卜微管蚜、丹参桃蚜、红花指管蚜的室内毒力。试验结果显示,阿维菌素对3种中草药蚜虫的室内毒力最高,其LC50值分别为9.125、0.730、1.135 mg/L;其次为印楝素,LC50值分别为103.425、2.318、2.483 mg/L;乙基多杀菌素、苦参碱对3种中草药蚜虫的室内毒力较低。阿维菌素、印楝素是防治中草药蚜虫的较好药剂,进一步做田间试验后可在生产上推广应用。 相似文献
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9种杀虫剂对芒果蓟马的毒力测定及田间防效 总被引：1，自引：0，他引：1
为弄清常用药剂对芒果蓟马的毒力及田间防效,在室内采用叶管药膜法,分别测定了9种常用农药对茶黄蓟马2龄若虫及成虫的毒力,并开展了田间防效试验。室内结果表明:9种药剂对茶黄蓟马2龄若虫和成虫的LC_(50)以1.5%甲氨基阿维菌素苯甲酸盐EC的最低,分别为0.90、0.91 mg/L;其次为6%乙基多杀菌素SC,分别为0.91、0.92 mg/L;LC_(50)以4.5%高效氯氰菊酯最高,分别为471.38、1626.92 mg/L。除2.8%阿维菌素外,其余供试药剂对茶黄蓟马2龄若虫的LC_(50)值都小于成虫,说明茶黄蓟马2龄若虫对上述供试药剂的敏感度均大于成虫。田间结果表明:10%啶虫脒ME、6%乙基多杀菌素SC、1.5%甲氨基阿维菌素苯甲酸盐EC、480 g/L毒死蜱EC、20%吡虫啉SL及12.5%阿维菌素·啶虫脒ME供试浓度对芒果蓟马复合种具有良好的速效性;22.4%螺虫乙酯SC供试浓度对芒果蓟马复合种的速效性较差,但添加哈速虅助剂后的持效性较好,可考虑与速效性较好且相溶性好的药剂合理混配使用,以提高药剂对芒果蓟马复合种的综合防效。 相似文献
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豇豆蓟马发生规律及防治药剂筛选的研究 总被引：1，自引：0，他引：1
为探明在冬种豇豆的整个生育期内,为害豇豆的蓟马种类及各种蓟马的种群消长情况,于2014—2016年在海南三亚开展了冬种豇豆的蓟马种类和数量的调查。结果表明:为害豇豆的蓟马有4种,即豆大蓟马、花蓟马、棕榈蓟马和黄胸蓟马;其中,豆大蓟马与棕榈蓟马在豇豆的整个生育期内均可为害,花蓟马与黄胸蓟马仅在花期发生。豆大蓟马为豇豆蓟马的优势种,其发生高峰期在豇豆的盛花期;其次是花蓟马,其数量在豇豆生长后期逐渐增加;棕榈蓟马的数量在豇豆花期虽有所增加,但始终维持在较低水平;黄胸蓟马仅偶尔出现,且数量极少。为了解新型杀虫剂对豇豆蓟马的防效,采用喷雾法,开展了豇豆蓟马的田间药效试验。试验结果表明:在参试的5种新型药剂中,乙基多杀菌素和苦参碱对豇豆蓟马具有较好的防效,药后3天防效分别为79.14%和62.14%,高于对照药剂啶虫脒的防效。药后7天,所有参试药剂对豇豆蓟马的防效均不理想,除了乙基多杀菌素,其余药剂防效均小于50%。在参试的药剂中,乙基多杀菌素、苦参碱对豇豆蓟马具有较好的防效,可以与啶虫脒轮换使用,用于豇豆蓟马的田间防治。 相似文献
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<正>科迪华宣布经董事会批准,公司将投资1.45亿美元扩增其位于密歇根州米德兰的生产设施产能,满足公司对天然虫害管理产品Spinosyns(含多杀霉素、乙基多杀菌素)的高需求。产能扩增项目是科迪华对多杀霉素、乙基多杀菌素两产品需求提升的响应,预计未来几年内完成。该项目可将现有产能提高 相似文献
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【目的】本研究旨在科学合理地利用浅层咸水资源。【方法】依托短期定位试验开展了在第3年和第4年不同咸水利用方式下(CK:造墒和蕾期灌淡水;T1:造墒和蕾期灌咸淡混配矿化度3 g·L^-1的微咸水;T2:淡水造墒蕾期灌矿化度5 g·L^-1咸水;T3:造墒和蕾期灌矿化度5 g·L^-1咸水;T4:淡水造墒蕾期不灌水)棉花长势、叶绿素荧光参数、土壤盐分累积及其运移的变化。【结果】T1和T2处理的齐苗率、株高、干物质质量、叶面积指数、叶绿素荧光参数、产量及霜前花率与CK无显著差异,土壤盐分含量较CK有所增加,但未对棉花生长产生明显抑制。T3处理的棉花长势指标、叶绿素荧光参数较CK显著降低,0~100 cm土壤盐分含量较CK明显增加。【结论】从土壤质量安全和咸水高效利用的角度考虑,连续4年用3 g·L^-1的咸淡混合水灌溉或用淡水与5 g·L^-1的咸水轮灌不仅能节约淡水,且不影响棉花产量。本研究结果为当地在棉花生产中安全利用咸水提供技术参考。 相似文献
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Chen Li Liu Liantao Ma Tongtong Jiang Dan Sun Hongchun Zhang Yongjiang Zhang Ke Bai Zhiying Li Cundong 《棉花学报》2019,31(5):438-447
[Objective] Cotton (Gossypium hirsutum L.) seed germination, in which melatonin plays an important regulatory role, is seriously affected by soil salinization. Cotton seed germination, antioxidant enzyme activity levels and other physiological indicators were analyzed to clarify the regulatory effects of exogenous melatonin on cotton seed germination under salt-stress conditions. [Method] The experiment was conducted in a greenhouse of Hebei Agricultural University in Baoding City, Hebei Province from 2018 to 2019 using Guoxin Cotton 9 as the material. The germination rate, germination potential, seed biomass after germination, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities, as well as the malondialdehyde (MDA) content, of cotton seeds were measured after exposure to different melatonin concentrations (0, 10, 20, 50 and 100 μmol·L－1) and 150 mmol·L－1 NaCl treatments. [Result] After germination, the germination rate, germination potential, radical length and seed biomass significantly decreased, as did the SOD, POD and CAT activities. However, the MDA content significantly increased under salt-stress conditions. Low exogenous melatonin concentrations (10 and 20 μmol·L－1) increased the germination potential, germination rate and biomass of cotton seeds, and promoted the elongation of radicals; however high melatonin concentrations (50 and 100 μmol·L－1) inhibited cotton seed germination and decreased seed biomass after germination. Low melatonin concentrations (10 and 20 μmol·L－1) increased the SOD, POD and CAT activities and decreased the MDA content in cotton seeds. [Conclusion] Low melatonin concentrations could promote germination and improve the salt tolerance of cotton seeds, while high melatonin concentrations could inhibit their germination. A 20 μmol·L－1 melatonin concentration is appropriate for regulating cotton seed germination under salt-stress conditions. 相似文献
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【目的】研究脱落酸(Abscisic acid, ABA)对棉花体细胞胚胎发生过程中下胚轴脱分化和再分化的影响,优化体细胞胚胎发生体系和初步解析脱落酸调控棉花体细胞胚胎发生分子机制。【方法】以棉花品种中棉所24(CCRI 24)下胚轴为外植体,设置5个ABA浓度0、0.02、0.04、0.06、0.08μmol·L^-1,分别以A0、A1、A2、A3、A4表示,添加至MSB(MS培养基+B5维生素)培养基诱导愈伤和胚性愈伤,研究ABA对棉花下胚轴初始细胞脱分化、愈伤组织诱导和胚性愈伤组织诱导的影响。【结果】ABA促进下胚轴初始细胞脱分化;显著提高愈伤组织的脱分化率和增殖率;0.02μmol·L^-1ABA显著提高胚性愈伤分化率,0.04~0.08μmol·L^-1ABA显著降低胚性愈伤分化率。ABA处理后胚性愈伤和非胚性愈伤的增殖率均显著提高且质地受到影响。0.02~0.08μmol ABA处理下,LBD和LBD在愈伤起始期上调表达。0.02μmol·L^-1ABA处理下,在愈伤增殖早期和中期BBM、LEC1和AGL15上调表达,愈伤增殖后期FUS3、LEA、ABI3基因上调表达。【结论】脱落酸调控的棉花体细胞胚胎发生与相关标记基因的时空性表达密切相关,这些基因表达水平的增加是ABA调控愈伤和胚性愈伤分化的分子基础。 相似文献
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Fu Huijie Xue Guojuan Liao Baopeng Qi Haikun Xu Dongyong Tan Weiming Du Mingwei Tian Xiaoli Li Zhaohu 《棉花学报》2021,32(6):561-568
[Objective] Early initiation and early maturity are the foundation of high yield and good quality of cotton. The purpose of this study is to determine the effects of plant growth regulators applied at the seedling and squaring stage on the early initiation of flower bud and the rate of the opened cotton boll (ROCB) during later development period, and to provide practical measures for hastening the maturity of cotton. [Method] Several plant growth regulators were applied from cotyledonary to squaring stage under greenhouse and field conditions, water was used as the control. The first fruiting branch node (indicating the initiation of flower bud), the number of bud prior to blooming and the ROCB at mid-term of boll maturation period (23 September, 2017) were compared among treatments. [Result] Under greenhouse conditions, gibberellic acid (GA3) applied at the cotyledonary stage with 140 μmol·L－1 as well as the three consecutive applications of sodium nitrophenolate (CSN, 2.23 μmol·L－1) at the cotyledonary, two-leaf and four-leaf stage made the first fruiting branch node move down by about 0.9 nodes. In field experiments, the application of gibberellin4+7(GA4+7, 288 and 576 μmol·L－1) at the cotyledonary stage significantly decreased the first fruiting branch node by about 0.4 nodes. Also, the application of 6-benzylaminopurine (6-BA, 44.4 μmol·L－1) at the three-leaf stage significantly decreased the first fruiting branch node by 0.2 nodes. However, there was no significant correlation between the first fruiting branch node and the ROCB in late September. Moreover, the application of Brassinolide (BR, 0.10 μmol·L－1) at the bud stage increased the ROCB in late September, which was mainly associated with the increased boll set in the lower and middle fruiting branches. [Conclusion] The reasonable distribution of bolls (concentrated in the lower and middle fruiting branches as well as inner fruiting sites) is more important for the earliness of cotton than lowering the first fruiting branch node. 相似文献
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[Objective] This study investigates the effects of exogenous proline on the growth, physiological responses, and proline metabolism of cotton (Ekang No.8) seedlings treated with varying boron (B) concentrations. [Method] We conducted a randomized experiment with six treatments using the hydroponic method in a greenhouse at Huazhong Agricultural University. We applied three different concentrations of boron, including 0.02 μmol·L－1 (low-concentration B), 2.5 μmol·L－1 (medium-concentration B), and 100 μmol·L－1 (sufficient-concentration B). The first two are boron-deficient treatments. We also applied an exogenous proline treatment at 20 μmol·L－1(0 μmol·L－1 as control). When any significant difference among the treatments were observed, the related indicator was measured. [Result] The results showed that exogenous proline inhibited the growth of cotton seedlings under sufficient-concentration B treatment while promoting the absorption of B by roots under low-concentration B treatment. The application of exogenous proline to the seedlings under low-concentration B treatment reduced the contents of proline and H2O2 in leaves but increased the accumulation of MDA and H2O2 in roots. The activities of SOD and antioxidant enzymes (APX) in the roots and leaves were dramatically enhanced. Conversely, POD activity reduced significantly and there was no significant change in CAT activity relative to low-concentration B treatment. More importantly, we found that the application of exogenous proline under B deficiency increased the activities of P5CS, P5CR, OAT (synthetase), and PRODH (degrading enzyme) in proline metabolic pathways. [Conclusion] Applying exogenous proline under sufficient-B concentration inhibits growth. The application of proline to seedlings under low-B concentration promotes the absorption of B by roots, increases the activity of APX, and decreases the membrane lipid peroxidation in leaves. A B deficiency leads to proline accumulation in plants. The addition of proline under low-B concentration can reduce the proline content in leaves, which is caused by affecting the critical synthetase and catabolic enzyme activities in the proline metabolism pathway (Glu and Orn pathway). The main reason for this occurrence is the significant increase of proline dehydrogenase (PRODH) activity. 相似文献
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Li Shuye Ma Huijuan Zhang Siping Liu Shaodong Shen Qian Chen Jing Ge Changwei Pang Chaoyou Zhao Xinhua 《棉花学报》2018,30(3):252-260
[Objective] The objective of this study was to investigate the effects of exogenous 24-epibrassinolide (EBR) on the photosynthetic physiology of cotton seedlings under low temperature and to provide basis for improving the cold tolerance of cotton by using EBR as growth regulator. [Method] Taking CCRI 60, Lumianyan 28 and Simian 3 as materials, a field experiment was carried out in Institute of Cotton Research of CAAS(Anyang county, Henan province). Before the first low temperature treatment, the cotton seedlings were sprayed with distilled water (Control) and different concentrations of EBR (0.1 mg·L－1 and 0.2 mg·L－1), respectively. After 3 days, the relative electrical conductivity, chlorophyll content, rapid chlorophyll fluorescence induction kinetic curve (OJIP) and fluorescence parameters were measured. [Result] Under low temperature, the relative conductivity of CCRI 60, Lumianyan 28 and Simian 3 spraying with EBR decreased by 17.7%～32.8% compared with control, and there was no significant difference between CCRI 60 and Lumianyan 28 in different concentrations of EBR treatments, butthe relative conductivity of Simian 3 treating with 0.2 mg·L－1 EBR was significantly lower than those treatments with 0.1 mg·L－1 EBR . The chl a and chl b contents increased by 9.7%～32.6% and 15.0%～18.9%, respectively. The maximum photochemical efficiency of photosystemⅡ (Fv/FM) and photosynthetic performance index on absorption basis(PIABS) increased significantly. PIABS of CCRI 60 increase the maximum by 75.6% using 0.1 mg·L－1 EBR. Lumianyan 28 and Simian 3 increased the maximum by 101.1% and 265.6% using 0.2 mg·L－1 EBR, respectively; Absorbed photon flux per cross section (ABS/CSm), electron transport flux (further than QA) per active reactive center (ETo/RC) and probability for electron transport (φEo) are significantly increased. [Conclusion] Exogenous EBR can enhance the ability of low temperature tolerance of cotton seedlings and alleviate the inhibition of photosynthesis in cotton at low temperature. The study showed that 0.1 mg·L－1 EBR performs well in CCRI 60 and 0.2 mg·L－1 in Lumianyan 28 and Simian 3. 相似文献
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[Objective] The purpose of this study was to expand upland cotton genotypes suitable for regeneration via somatic embryogenesis, so as to provide excellent receptor materials for the genetic transformation of cotton. [Method] Six different hormone combinations were studied to establish a somatic embryo regeneration system for hypocotyl explants of Xinluzao 45, a widely planted variety in Xinjiang Province. [Result] Faint yellow callus was induced on a medium consisting of hormone combinations C1 (0.1 mg·L－1 acetic acid dichlorobenzene oxide [2, 4-D] + 0.1 mg·L－1 kinetin [KT]) or C5 (0.1 mg·L－1 2, 4-D + 0.5 mg·L－1 indo-3-butyric acid + 0.1 mg·L－1 KT). The generated callus was able to successfully induce embryogenic callus on hormone-free medium containing doubled KNO3. The embryogenic callus then developed into somatic embryos and regenerated plants. Maximum rates of callus induction and regeneration were 92.2% and 40.3%, respectively. Although no significant difference was observed in the rate of callus induction of Xinluzao 45 hypocotyls between hormone combinations C1 and C5, the incidence of induced embryonic callus was higher on C1 than on C5. [Conclusion] The somatic embryo regeneration system developed in this study provides technical support that will facilitate Agrobacterium-mediated transformation of upland cotton Xinluzao 45. 相似文献
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Qi Haikun Wang Sai Xu Dongyong Lu Zhengying Zhao Wenchao Hao Yanjie Zhang Xiang Li Wei Han Huanyong Wang Jiangtao Wang Hongzhe Chen Hongzhang Wang Lin Du Mingwei Tian Xiaoli Li Zhaohu 《棉花学报》2013,32(5):425-437
[Objective] The objective of this study was to investigate the stability and universality of cotton chemical topping by applying mepiquat chloride (1,1-dimethyl-piperidinium chloride, DPC) in different cotton-growing regions. [Method] Field experiments were conducted in 2018 at 10 locations in the Yellow River basin (Hejian and Handan, Hebei province; Dezhou and Wudi, Shandong province), the Yangtze River basin (Dafeng, Jiangsu province; Huanggang, Hubei province), and Xinjiang area (Shihezi location I and loacation II, northern Xinjiang and Luntai and Shaya, southern Xinjiang). Local cultivars/lines were used, and the experiments were performed using a randomized complete block design with three or four replicates. Accompanied with typical DPC multi-application in each location, chemical topping was conducted at 10 days before manual topping (T1) or at the same time with manual topping (T2) by applying four dosages of DPC (0, 90, 180, 270 g·hm－2), manual topping was used as the first control and non-topping as the second control. [Result] The time of chemical topping significantly affected cotton plant height (except for the results in Handan, Dezhou and Wudi) and the number of fruit branches (except for the results in Dafeng and Huanggang). It was observed that earlier chemical topping would result in lower cotton plant height and a fewer fruit branches. In Hejian and Shihezi location I, the average plant height across DPC chemical topping at T1 stage was not only lower than that of T2 stage but also 3.3 cm and 4.6 cm lower than that of manual topping, respectively. In most locations, chemical topping at T1 stage increased around two fruit branches per plant compared with manual topping, while in T2 stage the increased fruit branches per plant ranged from 2.3 to 7.7. Also, we found that a higher dosage of DPC resulted in shorter plant height (except for that in Huanggang). In some locations, plant heights of chemical topping with 180 g·hm－2 or 270 g·hm－2 DPC were even shorter than that of manual topping. The number of fruit branches per plant of 0 g·hm－2 DPC increased by 2.4-8.3 compared with manual topping. However, chemical topping with 90-270 g·hm－2 DPC significantly reduced the number of fruit branches compared with 0 g·hm－2 DPC. There were no significant differences in the number of fruit branches among three DPC dosages (90, 180, and 270 g·hm－2). In Handan, seed cotton yield of chemical topping at T2 stage was significantly lower than that of manual topping due to the decreased boll number, which is possibly associated with the high temperature and drought weather after chemical topping. While at other locations, most treatments of chemical topping by using DPC did not produce significant effects on yield. In addition, chemical topping by using DPC did not delay cotton maturity, characterized by their similar boll-opening rate and the first harvest rate to those of manual topping before spraying harvest aids. [Conclusion] Cotton chemical topping with DPC is more stable and universal across different cotton-growing regions. We suggest that 90-180 g·hm－2 DPC could be used at the same time with manual topping for cotton chemical topping. 相似文献
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江苏滨海盐碱地麦后直播棉氮、磷、钾肥料优化配比研究 总被引：4，自引：0，他引：4
【目的】建立江苏省滨海盐碱地麦后直播棉合理的施肥技术体系。【方法】2017―2018年在江苏省滨海棉田,以中棉所50为材料,采用正交设计,研究了不同氮、磷、钾肥配施量对棉花生物量、养分累积与利用及产量的影响。【结果】氮、磷、钾肥3因子对棉株地上部和生殖器官生物量、棉株地上部氮和钾素累积量及皮棉产量的影响均为氮肥>磷肥>钾肥。施N 150~225 kg·hm-2、P2O575 kg·hm-2下棉株地上部和生殖器官生物量、氮和钾累积量及皮棉产量较高。钾肥因子对生殖器官生物量和皮棉产量影响不显著。钾肥因子对氮、钾素利用效率的影响大于氮肥和磷肥,氮肥因子对磷素利用效率的影响大于磷肥和钾肥,施K2O 75~150 kg·hm-2氮、钾素利用效率较高、施氮225 kg·hm-2磷素利用效率较高。土壤碱解氮、速效磷、速效钾含量较高棉田的氮、磷(P2O5)、钾(K2O)肥配施量分别为225 kg·hm-2、75 kg·hm-2和150~225 kg·hm-2。相关分析表明,皮棉产量与棉株氮、钾素累积量显著正相关。【结论】长江流域棉区滨海盐碱地麦后直播棉利于产量和养分利用效率提高的最佳氮、磷(P2O5)、钾(K2O)肥配施量分别为225 kg·hm-2、75 kg·hm-2、75 kg·hm-2。 相似文献
20.
Effects of Planting Density and Chemical control on Yield and Mineral Elements Accumulation of Cotton Direct-Seeded after Wheat 总被引：1，自引：1，他引：0
Wu Xinling Geng Jijia Cao Lifang Lin Yuan Liu Xiaofei Cai Zezhou Chen Yuan Zhang Xiang Chen Dehua 《棉花学报》2013,32(5):438-448
[Objective] The effects of planting density and Miantaijin treatment on yield and nitrogen, phosphorus and potassium uptake and accumulation in cotton direct-seeded after wheat in the Yangtze River basin were studied in order to clarify the high-yield cultivation techniques under this cropping system. 【Method】 In 2011 and 2012, Guoxinzao 11-1 at the experimental material, and adopted three densities (75 000, 90 000 and 105 000 plants·hm－2) and three Miantaijin doses (0 mL·hm－2, 1 170 mL·hm－2 and 2 340 mL·hm－2). 【Result】 The results showed that the highest yield was 3 551.3～3 687.5 kg·hm－2 under 90 000～105 000 plant·hm－2 density combined with 1 170 mL·hm－2 Miantaijin (seedling stage: 90 mL·hm－2, peak squaring stage: 180 mL·hm－2, peak flowering stage: 360 mL·hm－2, peak boll-setting stage: 540 mL·hm－2). Under these conditions, the highest uptake of nitrogen, phosphorus and potassium was 117.8 kg·hm－2, 77.4 kg·hm－2, 116.4 kg·hm－2, respectively. Among them, nitrogen uptake was the highest in the peak-squaring stage to peak-flowering stage, while the highest phosphorus and potassium uptake were both detected in the peak-flowering to boll-opening stage. The correlation analysis showed that there was a significant linear positive correlation between the yield of direct seeded cotton after wheat and the total absorption and accumulation of nitrogen, phosphorus and potassium, especially during the peak flowering to boll-opening stage. 【Conclusion】 The suitable application dose of Miantaijin under medium and high density could enhance the absorption of nitrogen, phosphorus and potassium in the whole growth period of cotton, especially in the peak flowering to boll-opening stage. and thus result in the high yield of direct-seeded cotton after wheat in the Yangtze River basin. 相似文献