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1.
【目的】豆科与禾本科间作体系中对磷有效性的影响主要集中在根系分泌物的活化作用,由根际沉淀引起的土壤碳含量与磷酸酶活性变化及其对红壤磷有效性的影响机制尚不清楚。【方法】本研究以间作玉米大豆为研究对象,设置根系完全分隔、尼龙网分隔、不分隔3种方式,在0、21.83、43.67、65.50和87.34 P mg kg-1（分别记为P0、P1、P2、P3和P4）磷肥施用水平下进行盆栽试验,研究根系分隔方式对间作玉米大豆根际土壤微生物量碳（MBC）、溶解性有机碳（DOC）、根际土壤有机碳（ROC）、酸性磷酸酶活性（ACP）、碱性磷酸酶活性（ALP）、速效磷和Hedley磷组分的影响。【结果】相比完全分隔,根系不分隔可提高玉米和大豆根际土壤MBC含量,显著降低玉米根际土壤DOC含量,低磷水平（P0、P1）时显著提高大豆DOC含量,显著提高玉米（仅在低磷时）和大豆根际土壤ACP活性,低磷时显著提高大豆根际土壤ALP活性。除玉米活性磷组分外,根系分隔方式对间作玉米大豆根际土壤速效磷、磷组分有显著或极显著影响。根系不分隔较完全分隔可通过降低大豆根际活性无机磷（Pi）(P0除外)和中活性Pi从而提高玉米根...  相似文献   

2.
The leguminous cover crops Atylosia scarabaeoides (L.) Benth., Centrosema pubescens Benth., and Pueraria phaseoloides (Roxb.) Benth., were grown in the interspaces of a 19 y–old coconut plantation and incorporated into the soil at the end of the monsoon season every year. At the end of the 12th year, soils from different depths were collected and analyzed for various microbial indices and their interrelationships. The objectives were to assess the effects of long‐term cover cropping on microbial biomass and microbial‐community structure successively down the soil profile. In general, total N (TN), organic C (OC), inorganic N, extractable P, and the levels of biological substrates viz., dissolved organic C (DOC) and N (DON), labile organic N (LON), and light‐fraction organic matter (LFOM) C and N decreased with depth at all the sites. Among sites, the cover‐cropped (CC) sites possessed significantly greater levels of TN, OC, DOC, DON, and LON compared to the control. Consequently, microbial biomass C (MBC), N (MBN), and P (MBP), CO2 evolution, and ATP levels, in general, decreased with depth at all sites and were also significantly higher in the CC sites. Among the ratios of various microbial indices, the ratio of MBC to OC and metabolic quotient (qCO2) declined with depth. Higher MBC‐to‐OC ratios and large qCO2 levels in the surface soils could be ascribed to greater levels of readily degradable C content and indicated short turnover times of the microbial biomass. In contrast, the ratios of MBC to MBN and MBC to MBP increased with depth due to low N/P availability and relatively higher C availability in the subsoils. Cover cropping tended to enhance the ratios of MBC to OC, MBC to MBN, MBC to MBP, and ergosterol to MBC and decreased the ATP‐to‐MBC ratio at all depths. The relatively lower ATP‐to‐MBC ratios in the CC site, especially in the subsoil indicated microbial‐community structure possibly dominated by fungi. By converting the ergosterol content to fungal biomass, it was observed that fungi constituted 52%–63% of total biomass C at the CC site, but only 33%–40% of total biomass C at the control site. Overall, the study indicated that leguminous cover crops like P. phaseoloides or A. scarabaeoides significantly enhanced the levels of OC, N and microbial activity in the soils, even down to 50 cm soil depth.  相似文献   

3.
ABSTRACT

The effectiveness of plant–soil synergies is largely modulated by interaction between cultivar and rhizosphere microbiome. We evaluated the agronomic performance of six durum wheat cultivars, in two semi-arid locations in Tunisia that differed in their irrigation water salinity: S1 (6 dS m?1) and S2 (12 dS m?1). The two-consecutive-year field experiments assessed the effects of the microbial biomass carbon (MBC), leaf phosphorus (LP) and rhizosphere phosphorus (P) on the grain yield (GY) and yield components at tillering and flowering stages. Overall, in saline conditions, cultivars differed in above- and below-ground traits, particularly, with tolerant cultivars presenting relatively greater MBC, P and LP. Furthermore, in S2, GY positively correlated with MBC (r = 0.69), LP (r = 0.80) and P (r = 0.79). Additionally, in S2, MBC positively correlated with P (r = 0.87) and LP (r = 0.85) at flowering. This result was further confirmed by multiple linear regression (step-wise) analysis, which revealed that MBC and LP were the determinant components of GY variability under S2. The present study demonstrates that LP and soil P are mandatory for improving the management of durum wheat. Salinity tolerance was largely affected by the cultivars’ rhizosphere MBC.  相似文献   

4.
The interactions between soil P availability and mycorrhizal fungi could potentially impact the activity of soil microorganisms and enzymes involved in nutrient turnover and cycling, and subsequent plant growth. However, much remains to be known of the possible interactions among phosphorus availability and mycorrhizal fungi in the rhizosphere of berseem clover (Trifolium alexandrinum L.) grown in calcareous soils deficient in available P. The primary purpose of this study was to look at the interaction between P availability and an arbuscular mycorrhizal (AM) fungus (Glomus intraradices) on the growth of berseem clover and on soil microbial activity associated with plant growth. Berseem clover was grown in P unfertilized soil (−P) and P fertilized soil (+P), inoculated (+M) and non-inoculated (−M) with the mycorrhizal fungus for 70 days under greenhouse conditions. We found an increased biomass production of shoot and root for AM fungus-inoculated berseem relative to uninoculated berseem grown at low P levels. AM fungus inoculation led to an improvement of P and N uptake. Soil respiration (SR) responded positively to P addition, but negatively to AM fungus inoculation, suggesting that P limitation may be responsible for stimulating effects on microbial activity by P fertilization. Results showed decreases in microbial respiration and biomass C in mycorrhizal treatments, implying that reduced availability of C may account for the suppressive effects of AM fungus inoculation on microbial activity. However, both AM fungus inoculation and P fertilization affected neither substrate-induced respiration (SIR) nor microbial metabolic quotients (qCO2). So, both P and C availability may concurrently limit the microbial activity in these calcareous P-fixing soils. On the contrary, the activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) enzymes responded negatively to P addition, but positively to AM fungus inoculation, indicating that AM fungus may only contribute to plant P nutrition without a significant contribution from the total microbial activity in the rhizosphere. Therefore, the contrasting effects of P and AM fungus on the soil microbial activity and biomass C and enzymes may have a positive or negative feedback to C dynamics and decomposition, and subsequently to nutrient cycling in these calcareous soils. In conclusion, soil microbial activity depended on the addition of P and/or the presence of AM fungus, which could affect either P or C availability.  相似文献   

5.
The effects of vermicompost (VC) (0% and 1% w/w) on treated calcareous clay soil with 0 and 50 mg phosphorus (P) kg?1 as calcium phosphate [Ca(H2PO4)2.H2O] was investigated. The soil samples were incubated for 7, 30, 60, 120, and 150 d at 25 ± 1°C and Olsen-P was measured after each incubation time. Results showed that Olsen-P increased 36% and 38% after VC addition in treated soil with 0 and 50 mg P kg?1, respectively. Recovery of Olsen-P in treated soils with VC, combined fertilizer VC + P, and fertilizer P was 42%, 42%, and 17%, respectively. The rate coefficient in treated soils with fertilizer, VC, and combined fertilizer VC + P was 0.033, 0.026, and 0.023 mg kg?1 d?1/2, respectively. It seems that the process that leads to the decrease in available P in amended soils, is controlled by P diffusion into sorption sites in micropores of aggregates.  相似文献   

6.
《Applied soil ecology》2011,47(3):341-346
We examined acid phosphatase activity (APA), N mineralization and nitrification rates, available N and P, and microbial biomass C, N and P in rhizosphere and bulk soils of 18-year-old Siberian elm (Ulmus pumila), Simon poplar (Populus simonii) and Mongolian pine (Pinus sylvestris var. mongolica) plantations on a nutrient-poor sandy soil in Northeast China. The main objective was to compare the rhizosphere effects of different tree species on N and P cycling under nutrient-deficient conditions. All tree species had the similar pattern but considerably different magnitude of rhizosphere effects. The APA, potential net N mineralization and nitrification rates increased significantly (by 27–60%, 110–188% and 106–142% respectively across the three species) in rhizosphere soil compared to bulk soil. This led to significantly higher Olsen-P and NH4+-N concentrations in rhizosphere soil, whereas NO3−-N concentration was significantly lower in rhizosphere soil owing to increased microbial immobilization and root uptake. Microbial biomass C and N generally increased while microbial biomass P remained constant in rhizosphere soil relative to bulk soil, indicating the N-limited rather than P-limited microbial growth. Rhizosphere effects on P transformation were most pronounced for Siberian elm, while rhizosphere effects on N transformation were most pronounced for Mongolian pine, implying the different capacities of these species to acquire nutrients.  相似文献   

7.
Drying and rewetting cycles are known to be important for the dynamics of carbon (C), phosphorus (P), and nitrogen (N) in soils. This study reports the short‐term responses of these nutrients to consecutive drying and rewetting cycles and how varying soil moisture content affects microbial biomass C and P (MBC and MBP), as well as associated carbon dioxide (CO2) and nitrous oxide (N2O) emissions. The soil was incubated for 14 d during which two successive drying–rewetting episodes were imposed on the soils. Soils subjected to drying (DRW) were rewetted on the seventh day of each drying period to return them to 60% water holding capacity, whilst continually moist samples (M), with soil maintained at 60% water holding capacity, were used as control samples. During the first seven days, the DRW samples showed significant increases in extractable ammonium, total oxidized nitrogen, and bicarbonate extractable P concentrations. Rewetting after the first drying event produced significant increases only in CO2 flux (55.4 µg C g?1 d?1). The MBC and MBP concentrations fluctuated throughout the incubation in both treatments and only the second drying–rewetting event resulted in a significantly MBC decrease (416.2 and 366.8 mg kg?1 in M and DRW soils, respectively). The two drying–rewetting events impacted the microbial biomass, but distinguishing the different impacts of microbial versus physical impacts of the perturbation is difficult. However, this study, having a combined approach (C, N, and P), indicates the importance of understanding how soils will react to changing patterns of drying–rewetting under future climate change.  相似文献   

8.
Evidence of the impacts of land-use change on soil biological activities, a determinant of nutrient cycling in soil, will provide a better understanding of soil health and productivity. The study investigates temporal changes in soil chemical and microbial properties in a forest converted to oil palm plantation. Soil samples from four locations: native forest and Elaeis guineensis plantations of 2-, 13- and 14-year were collected. Total carbon C and N were significantly higher (p < 0.05) in the native forest (NF) followed by 14- and 13-year E. guineensis plantations respectively. Microbial biomass carbon (MBC) and nitrogen (MBN) in NF were significantly higher (p < 0.05) than in the E. guineensis plantations. There were no significant differences in the microbial biomass phosphorus (MBP) among the E. guineensis plantation of all ages. The qCO2 in the 13- and 14-year E. guineensis plantations was higher than in NF and 2-year E. guineensis plantation. There was a positive correlation between MBC, MBN, MBP and pH, P, TC, and TN. These results indicated that conversion of native forest to E. guineensis plantation affected soil nutrient and microbial properties. And there could be a return to healthy soil condition as age of E. guineensis plantation increased.  相似文献   

9.
The main aim of this research work is to prepare an enriched compost using rice straw mixed with rock phosphate, waste mica and Aspergillus awamori and to study their effect on changes in microbial properties in soils with and without chemical fertilizers under wheat-soybean rotation. Data revealed that significant increase in microbial biomass carbon (MBC), dehydrogenase activity, phosphatase activities, and microbial biomass phosphorus (MBP) in soil were maintained in enriched compost than ordinary compost after both the crops. Significant increase in MBC, dehydrogenase activity, phosphatase activities, and MBP were found in surface soil. The maximum microbial activities were observed in the treatment receiving 50% recommended dose of fertilizer (RDF) + enriched compost at 5 t ha?1 indicating that integrated use of chemical fertilizers and enriched compost significantly improved the biological properties of soil under wheat–soybean rotation thereby enhanced soil fertility and crop production.  相似文献   

10.
Microbial biomass phosphorus (MBP) and its relationships with other biological and chemical properties were studied on loess soil with an 11-year long-term fertilization experiment. The results indicated that inorganic fertilizers (F) improved soil microbial biomass carbon (MBC), nitrogen (MBN), and MBP levels and F plus maize stalk (SNPK) improved MBC and MBN. Manuring markedly increased soil MBC, MBN, and MBP levels. Fertilization decreased the ratios of MBC/MBN, MBC/MBP, and MBN/MBP. Microbial biomass phosphorus was positively and linearly correlated with MBC, MBN, organic carbon (SOC), total phosphorus (TP), water-soluble P, and Olsen P but negatively correlated with soil pH. Microbial biomass phosphorus constituted 2% of TP on control (CK) and inorganic fertilizer treatments and 12% on manure plots. Microbial biomass phosphorus to Olsen P ratios were 50% on CK, F, and SNPK and 80% on manure treatments. Measurements of MBP in soil containing high Olsen P were subject to analytical problems of unknown reasons.  相似文献   

11.
Rhizosphere processes have a major impact on Zn desorption and Zn uptake by plant. However, information about Zn desorption characteristics in the rhizosphere of wheat is limited. Therefore, a greenhouse experiment was performed to determine Zn desorption characteristics in the bulk and rhizosphere soil of wheat (Triticum aestivum L.) of 10 soils amended and un-amended with municipal sewage sludge using rhizoboxes. The kinetics of Zn desorption was determined by successive extraction with 10 mM citric acid in a period of 1–504 h at 25 ± 1°C in the bulk and rhizosphere of un-amended and amended soils. The results showed that the Zn amount extracted after 504 h in the rhizosphere soils was significantly (p < 0.01) higher than the bulk soils. The mean of Zn desorption in the bulk and rhizosphere of un-amended soils were 10.4 ± 0.34 and 11.4 ± 0.43 mg kg?1, respectively, while the mean of Zn desorption in the bulk and the rhizosphere of amended soils were 13.2 ± 0.48 and 14.8 ± 0.67 mg kg?1, respectively. Desorption kinetics of Zn conformed fairly well to the first-order, parabolic diffusion, power function, and zero-order equations. The results of Zn fractionation indicated that exchangeable Zn and Zn associated with organic matter decreased and Zn associated with iron-manganese oxides and residual Zn increased in the rhizosphere soils compared to the bulk soils. Zinc desorption after 504 h and residual Zn in the bulk and rhizosphere of un-amended and amended soils were significantly positively correlated (p < 0.05). Therefore, residual Zn was the main Zn pool that controlled Zn desorption after 504 h in the bulk and rhizosphere soils studied.  相似文献   

12.
A greenhouse experiment was conducted to investigate the effect of phytoextraction by Chinese brake fern (Pteris vittata L.) on microbial activity and biomass in arsenic-contaminated soil (naturally occurring arsenic-contaminated soils of West Bengal, India). P. vittata was grown for two successive growing cycles (4 months each) with two phosphate sources (di-ammonium phosphate (DAP) and single superphosphate (SSP)). After phytoextraction by P. vittata, the rhizosphere soils were analyzed for microbial biomass C (MBC), C mineralization (Cmin), dehydrogenase activity (DHA), phosphomonoesterase activities, and aryl sulphatase activity. All enzyme activities increased after two successive growing cycles of P. vittata as compared to one growing cycle and unplanted control. The arsenic (As) phytoextraction by this fern also increased the MBC by 34 %, Cmin by 63 %, DHA by 38 %, acid phosphomonoesterase activity by 30 %, alkaline phosphomonoesterase activity by 6 %, and aryl-sulphatase activity by 33 % with two successive growing cycles over unplanted control. The di-ammonium phosphate was better as compared to single super phosphate for enhancing microbiological and biochemical parameters except phosphomonoesterase activities.  相似文献   

13.
  目的  探讨桂西北喀斯特峰丛洼地不同植被类型的土壤理化性质和微生物碳(MBC)、微生物氮（MBN）、微生物磷（MBP）含量的变化特征及它们之间的关系。  方法  利用生态化学计量方法和Pearson相关性分析方法研究不同植被类型和土层深度对土壤MBC、MBN、MBP含量和土壤养分含量分布特征的影响。  结果  （1）不同植被类型土壤养分含量和MBC、MBN、MBP含量依次为次生林 > 灌木 > 灌草 > 草地 > 耕地;土壤养分垂直分布表现为随着土层深度加深而下降,不同土层间土壤有机碳（SOC）、全氮（TN）、全磷（TP）含量差异显著,土壤MBC、MBN和MBP含量在不同植被类型和不同土层间差异显著,均表现为MBC > MBN > MBP。（2）不同植被类型土壤MBC/SOC和MBP/TP的值较小,MBN/TN的差异较大。不同植被类型的土壤MBC/MBN差异显著,MBC/MBP变化范围较大,MBN/MBP表现为次生林 > 灌草 > 灌木 > 草地 > 耕地。（3）土壤MBC和MBN与SOC、TN、速效氮和速效钾呈显著或极显著正相关,与土壤容重、pH值表现出不同程度的负相关,表明植被恢复过程中土壤MBC和MBN可作为衡量土壤养分的敏感性指标。  结论  不同植被类型的土壤微生物生物量碳氮磷、养分含量和化学计量特征有明显的表聚效应,随着植被的正向演替,土壤结构、养分和微生物群落功能得到显著提高。  相似文献   

14.
The primary factor that influences release of zinc (Zn) for plants is the rhizosphere. However, information about Zn-desorption characteristics in the rhizosphere is limited. A greenhouse experiment was performed to determine Zn-release characteristics in the bulk and the rhizosphere soils using a rhizobox. The kinetics of Zn release was determined by successive extraction with diethylenetriaminepentaacetic acid (DTPA)– triethanolamine (TEA) in a period of 1 to 504 h at 25 ± 1 °C in the bulk and the rhizosphere soils. Moreover, Zn extracted by using three extractants [DTPA-TEA, ammonium bicarbonate (AB)-DTPA, and Mehlich 3] in the bulk and the rhizosphere soils. The results showed that Zn extracted in the rhizosphere soils were significantly (P < 0.01) lower than the bulk soils. The mean of Zn release in the bulk and the rhizosphere soils were 5.31 and 4.91 mg kg?1, respectively. Release kinetics of Zn conformed fairly well to power function, first order, parabolic diffusion, and simplified Elovich equations. The results of kinetics study indicated that release-rate coefficients decreased in the rhizosphere soils compare to the bulk soils. The correlation studies showed that Zn release after 504 h was significantly correlated (P < 0.05) with Zn extracted by using DTPA-TEA, AB-DTPA, and Mehlich 3 in the bulk and the rhizosphere soils. The results of this research showed that Zn-release characteristics in the bean rhizosphere soils were different from the bulk soils.  相似文献   

15.
Rhizosphere processes have a major impact on copper (Cu) availability and its fractions in soils. A greenhouse experiment with wheat was performed to investigate availability (using seven chemical procedures) and fractionation of Cu in the rhizosphere of ten agricultural soils (Typic Calcixerepts) amended with sewage sludge (1% w/w) using rhizoboxes. The results show that available Cu concentrations in rhizosphere soils were significantly (P < 1%) lower than in bulk soils. In comparison with the bulk soils, in the rhizosphere soils the concentration of Cu associated with organic matter and residual Cu increased by 24 and 4%, respectively, whereas exchangeable Cu, Cu associated with iron‐manganese oxides, and Cu associated with carbonate decreased by 20, 14, and 12%, respectively. Dissolved organic carbon (DOC) and Cu associated with iron‐manganese oxides and Cu associated with organic matter in the rhizosphere and bulk soils were significantly correlated (P < 5%). The results show that the differences between rhizosphere and bulk soils in chemical conditions such as DOC concentrations can change the proportion of soil Cu fractions and, therefore, Cu availability for wheat in calcareous soils amended with sewage sludge. The results show that the wheat root‐induced modifications of chemical and biological soil conditions do not only lead to Cu depletion in mobile soil Cu fractions, but also to modification in soil Cu fractions which are commonly considered as more stable.  相似文献   

16.
Many questions have surfaced regarding long-term impacts of land-use and cultivation system on soil carbon (C) sequestration. The experiment was conducted at Ohio Agricultural Research and Development Center. Only minor variations of soil organic carbon (SOC) and nitrogen (N) fractions with depth under plow tillage (PT). The SOC, total nitrogen (TN), microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) concentrations were higher under grassland and forestland in the top 0–15 cm depth than arable soils. No-tillage (NT) also increased SOC and N fractions concentrations in the surface soils than PT. Compared to arable, grass and forest could significantly improve proportions of MBC and MBN, and reduce proportions of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON). NT and forest also increased the ratio of SOC/TN, MBC/MBN, and DOC/DON. Overall, grass and forest provided more labile C and improved C sequestration than arable. So did NT under arable land-use.  相似文献   

17.
  【目的】  探讨不同氮磷配施条件下土壤无机磷组分转化特征和无机磷组分转化的影响因素,为陇中黄土高原旱作农业区农田磷素的高效利用及农田养分平衡提供参考。  【方法】  基于2017年布设在陇中黄土高原定西市李家堡镇麻子川村的不同氮磷配施春小麦长期定位试验,氮 (N)、磷 (P2O5) 各设4个水平,分别为0、75.0、115.0、190.0 kg/hm2,两两正交共16个处理。使用顾益初-蒋柏藩法测定收获后耕层 (0―20 cm) 土壤中各形态无机磷组分含量以及环境因子 (土壤有机碳、全氮、全磷、有效磷、pH、籽粒产量、地上部生物量、磷肥回收利用率、微生物量碳、氮、磷和碱性磷酸酶)。  【结果】  土壤无机磷组分变化顺序为Ca10-P > Ca8-P > O-P > Fe-P ≈ Al-P > Ca2-P,无机磷含量主要以Ca-P为主,Al-P、Fe-P、O-P 3种形态占无机磷总量的20%左右。施磷显著增加土壤各无机磷组分含量,施氮显著降低除O-P、Ca8-P外其它无机磷组分含量,使O-P显著增加。施氮对各无机磷组分比例变化影响较小,Ca2-P、Ca8-P占无机磷总量的比例随着施磷量的增加而增加,Ca10-P、O-P所占的比例随着施磷量的增加呈下降趋势。Fe-P占无机磷的比例随着施磷量的增加基本无变化。本研究土壤有效磷与Ca2-P、Ca8-P、Fe-P、O-P之间呈极显著正相关 (P < 0.01),与Al-P呈显著正相关 (P < 0.05),与Ca10-P相关性不显著 (P > 0.05)。通径分析结果显示,各形态无机磷对有效磷的直接贡献顺序为Ca2-P > O-P > Al-P > Ca10-P > Fe-P > Ca8-P,在本区Ca2-P是土壤有效磷的主要磷源,Ca8-P、Fe-P是潜在磷源。施氮显著提高了土壤有机碳、全氮、籽粒产量、地上部生物量及微生物量碳、氮、磷及碱性磷酸酶活性和磷肥回收利用率,降低了全磷、有效磷、pH。施磷显著提高了全氮、全磷、有效磷、籽粒产量、地上部生物量及微生物量碳、氮、磷及碱性磷酸酶活性,降低了有机碳。冗余分析结果显示,土壤有机碳是影响陇中黄土高原旱作春小麦农田耕层土壤无机磷组分变化的关键因子;Ca8-P与全氮、Al-P与磷肥回收利用率、O-P与籽粒产量、Fe-P与地上部生物量和碱性磷酸酶活性以及微生物量氮呈极显著正相关,土壤有机碳与各无机磷组分均呈负相关。  【结论】  氮磷配施能够促进土壤磷素的活化,提高可供植物直接利用的Ca2-P和具有缓效作用Ca8-P、Al-P的比例,降低了土壤中难溶性Ca10-P、O-P的比例,提升了土壤潜在供磷能力。土壤有机碳是调控该区耕层土壤磷组分转化的关键因子。  相似文献   

18.
Earthworms and arbuscular mycorrhizal fungi (AMF) are known to independently affect soil microbial and biochemical properties, in particular soil microbial biomass (SMB) and enzymes. However, less information is available about their interactive effects, particularly in soils contaminated with heavy metals such as cadmium (Cd). The amount of soil microbial biomass C (MBC), the rate of soil respiration (SRR) and the activities of urease and alkaline phosphatase (ALP) were measured in a calcareous soil artificially spiked with Cd (10 and 20 mg Cd kg−1), inoculated with earthworm (Lumbricus rubellus L.), and AMF (Glomus intraradices and Glomus mosseae species) under maize (Zea mays L.) crop for 60 days. Results showed that the quantity of MBC, SRR and enzyme activities decreased with increasing Cd levels as a result of the elevated exchangeable Cd concentration. Earthworm addition increased soil exchangeable Cd levels, while AMF and their interaction with earthworms had no influence on this fraction of Cd. Earthworm activity resulted in no change in soil MBC, while inoculation with both AMF species significantly enhanced soil MBC contents. However, the presence of earthworms lowered soil MBC when inoculated with G. mosseae fungi, showing an interaction between the two organisms. Soil enzyme activities and SRR values tended to increase considerably with the inoculation of both earthworms and AMF. Nevertheless, earthworm activity did not affect ALP activity when inoculated with G. mosseae fungi, while the presence of earthworm enhanced urease activity only with G. intraradices species. The increases in enzyme activities and SRR were better ascribed to changes in soil organic carbon (OC), MBC and dissolved organic carbon (DOC) contents. In summary, results demonstrated that the influence of earthworms alone on Cd availability is more important than that of AMF in Cd-polluted soils; and that the interaction effects between these organisms on soil microorganism are much more important than on Cd availability. Thus, the presence of both earthworms and AMF could alleviate Cd effects on soil microbial life.  相似文献   

19.
磷肥减施对玉米根系生长及根际土壤磷组分的影响   总被引：5，自引：1，他引：4  
【目的】 我国农业过量和不合理施用磷肥现象普遍存在,导致磷资源的浪费,对环境也造成潜在威胁。研究减少磷肥用量对玉米产量、根系形态及根际中磷转化特征的影响,为集约化农业生产体系中磷肥合理施用提供技术基础。 【方法】 在河北省衡水小麦玉米轮作体系下连续三年进行了田间试验,在冬小麦季设置4个P2O5用量处理:0、112.5、150.0、187.5 kg/hm2,收获后在原处理小区免耕播种夏玉米。利用WinRHIZO根系分析系统分析获取根长、直径等数据,测定玉米籽粒产量、生物量和地上部磷含量及根际土壤中磷形态等指标。 【结果】 与农民习惯磷肥用量(P2O5187.5 kg/hm2)相比,3年磷肥用量减施20%~40%处理(P2O5150和112.5 kg/hm2),玉米籽粒产量、根系长度与直径和土壤有效磷含量尚未发生明显变化。但3年不施磷处理,根际土壤有效形态磷含量和玉米籽粒产量开始出现下降趋势。2009年和2010年玉米收获期,不施磷肥处理根际土壤有机磷含量低于非根际土壤。2008年玉米苗期和收获期土壤有机磷分组中,中等活性有机磷含量最高;磷肥减施20%~40%处理苗期根际中中等活性有机磷含量显著低于非根际土壤。土壤无机磷形态分组研究发现:从玉米苗期到收获期,各磷肥处理根际和非根际土壤中Ca2-P下降明显;而不同磷肥处理间土壤中Ca10-P、Ca8-P、O-P (闭蓄态磷)、Al-P和Fe-P含量差异不显著。减施磷肥处理2008年玉米苗期根际土壤微生物量P含量较非根际土壤高;与习惯施肥量相比,磷肥减施未明显降低根际土壤微生物量磷。 【结论】 在华北小麦玉米轮作种植体系下,在土壤肥力水平较高地区,连续3年将小麦季磷肥的习惯用量减少20%~40%,对夏玉米产量、根系形态以及根际土壤无机磷、有机磷、微生物量磷含量影响尚不明显,因此,该地区磷肥施用量可从习惯用量的P2O5180 kg/hm2减至112.5 kg/hm2。  相似文献   

20.
Zhang  Wenyuan  Liu  Shun  Zhang  Manyun  Li  Yinan  Sheng  Keyin  Xu  Zhihong 《Journal of Soils and Sediments》2019,19(7):2913-2926
Purpose

Rhizosphere and fertilization might affect soil microbial activities, biomass, and community. This study aimed to evaluate the impacts of Phyllostachys edulis (moso bamboo) rhizospheres on soil nutrient contents and microbial properties in a moso bamboo forest with different fertilizer applications and to link soil microbial activities with abiotic and biotic factors.

Materials and methods

The experiment included three treatments: (1) application of 45% slag fertilizer (45%-SF); (2) application of special compound fertilizer for bamboos (SCF); and (3) the control without any fertilizer application (CK). Simultaneously, bulk soils and 0.5, 2.5, 4.5, and 6.5-year-old (y) bamboo rhizosphere soils were selected. Soil nutrient contents were analyzed. Microbial activities were evaluated based on the activities of soil enzymes including β-glucosidase, urease, protease, phosphatase, and catalase. The total microbial biomass and community were assessed with the phospholipid fatty acids (PLFAs) method.

Results and discussion

In the CK and SCF treatments, organic matter contents of rhizosphere soils were significantly higher than those of bulk soils. Soil β-glucosidase, urease, protease, phosphatase, and catalase activities in rhizosphere soils were higher than those of bulk soils, with the sole exception of β-glucosidase of 0.5 y rhizosphere soil in the 45%-SF treatment. Compared with the CK treatment, fertilizer applications tended to increase soil total PLFAs contents and changed soil microbial community. Moso bamboo rhizospheres did not significantly increase the total microbial biomass. In the SCF treatment, the Shannon index of bulk soil was significantly lower than those of rhizosphere soils.

Conclusions

Our results suggested that both rhizospheres and fertilizer applications could change the soil microbial community structures and that moso bamboo rhizosphere could increase microbial activity rather than biomass in the forest soils with different fertilizer applications.

  相似文献   

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