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1.
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2.
Prebiotic galactosyl oligosaccharides (GOS) are produced from lactose by the enzyme β-galactosidase. It is widely reported that the highest GOS levels are achieved when the initial lactose concentration is as high as possible; however, little evidence has been presented to explain this phenomenon. Using a system composed of the commercial β-galactosidase derived from Bacillus circulans known as Biolacta FN5, lactose and sucrose, the relative contribution of water activity, and substrate availability were assessed. Oligosaccharide levels did not appear to be affected by changes in water activity between 1.0 and 0.77 at a constant lactose concentration. The maximum oligosaccharide concentration increased at higher initial concentrations of lactose and sucrose, while initial reaction rates for transfer increased but remained constant for hydrolysis. This suggests that the high oligosaccharide levels achieved at the raised initial saccharide concentration are due to increases in reactions that form oligosaccharides rather than decreases in concurrent reactions, which degrade oligosaccharides. There were different effects from changing the initial concentration of lactose compared to sucrose, suggesting that the ability of lactose to act as a donor saccharide may be more important for increasing maximum oligosaccharide concentrations than the combined ability of both saccharides to act as galactosyl acceptors.  相似文献   

3.
One of the most serious environmental problems is the current acceleration in the rate of species extinction associated with human activities, which is occurring particularly rapidly in freshwaters. Here we examine whether endemic water beetles are effectively protected by existing conservation measures in the Iberian Peninsula and the Balearic Islands, a region of high diversity and intense human pressure. We used an exhaustive database for aquatic beetles in the region to address such issues. Firstly, we identify the most threatened endemic taxa using a categorization system to rank species according to their conservation priority or vulnerability. Of the 120 endemic species of water beetles used in the analysis, only two (Ochthebius ferroi and Ochthebius javieri) were identified as being extremely vulnerable, 71 were highly vulnerable and 46 moderately vulnerable, with only a single species identified as having low vulnerability status. Since no Iberian species of aquatic Coleoptera has legal protection, the only conservation measure available for these species is the extent to which they occur in protected areas. Here we identify distributional hotspots for threatened endemic species, and evaluate the extent to which these are already included in the Natura 2000 network in Spain and Portugal. Despite a high degree of concordance between hotspots and Natura 2000 sites, the distribution of four species falls completely outside the network. The analysis also reveals that Natura 2000 fails to protect saline water bodies, despite their high conservation interest and narrow global distribution. The picture revealed here with water beetles is likely to be similar for others groups of freshwater macroinvertebrates, since Coleoptera are known to be good surrogates of aquatic biodiversity in the region. Finally, the degree of protection provided via Natura 2000, and the utility of red lists are discussed.  相似文献   

4.
Hydraulic Resistance and Capacitance in the Soil-Plant System   总被引：1，自引：0，他引：1  
In this paper, the hydraulic resistances and capacitances were evaluated. based on the development of non-(?) model of water flow in the soil-plant system and the simulating experiment work.The results show that the mean hydraulic resistance in the soil-plant system is 6.79×109 MPa·S·m-3; the mean hydraulic capacitance in the system is 5.2×107m3·MPa-1. In the components of hydraulic capacitance in the system, the capacitance in soil (81.8×10-6m3·MPa ) is the biggest and its variability with suii water potential is extremely strong, the capacitance in plant (5.3×10-7m3·MPa-1) is much smaller than that in soil, and the capacitance in shoots (15.5×10-7m3·2MPa-1) is bigger than that in roots (8.4×10-7m3·2MPa-1). An interesting result is that the capacitance in plant is almost equivalent to that in the soil-plant system.  相似文献   

5.
Eurasian Soil Science - Acid–base characteristics and composition of clay minerals were estimated in the rhizospheres of Norway maple (Acer platanoides) and common spruce (Picea abies) and in...  相似文献   

6.
In this paper, we present the first detailed study of the reaction kinetics and the characterization of the products from the endothermic reactions between β-lactoglobulin and genipin. The effects of the concentration, temperature, and pH were investigated. In the temperature range studied, the reaction was approximately a pseudo-first-order with respect to genipin and 0.22-order and -0.24-order with respect to β-lactoglobulin for pH 6.75 and 10.5 with corresponding activation energy (E(a)) estimated to be 66.2 ± 3.8 and 9.40 ± 0.36 kJ/mol, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis studies, validated by matrix-assisted laser desorption ionization-time of flight mass spectrometry, showed the presence of oligomeric, i.e., di-, tri-, quadri-, and pentameric, forms of cross-linked β-lactoglobulin by genipin at neutral but not alkaline pH; however, an extensive cross-linked network was not observed, consistent with the atomic force microscopy images. It was demonstrated that the reaction temperature and the concentration of genipin but not that of β-lactoglobulin positively affected the extent of the cross-linking reactions.  相似文献   

7.
A local variety of tomato (Lycopersicon esculentum, Ife plum cv. 51691) was grown in soil culture for 5 months and treated with B at concentrations of 0, 1, 2, 4, 8, and 16 ppm as H3BO3, and Ca at 0, 40, 80, and 160 ppm as Ca(OH)2. A significantly positive correlation was established between organic matter and water ‐ soluble B (r = 0.970), while the relationship between pH and B was negative (r = ‐0.490). Application of B at 2 ppm improved all growth parameters studied. Boron application higher than 2 ppm, induced leaf chlorosis and later necrosis of nodes and roots. Fruit yield correlated positively with soil ‐ B, stem diameter and floral number (r = 0.597, r = 0.650 and r = 0.812, respectively). Soil‐ and plant‐B were positively correlated (r = 0.790). Calcium when applied singly at higher levels increased total chlorophyll content of the leaf. Tomato fruit yield was optimum at B:Ca treatment concentration of 2 ppm B (4.48 kg/ha B) and 160 ppm Ca (358.4 kg/ha Ca), corresponding to a B:Ca fertilizer ratio of 1.80.  相似文献   

8.
The changes in the material composition of the buried soils and loesses in relation to the dynamics of the climate and sediment accumulation were studied for revealing the pedogenetic features and assessing the natural conditions in the steppe zone of the southern Russian Plain. A comparative analysis of the chemical compositions of the different-aged Pleistocene loess-soil complexes (the Otkaznoe, Port-Katon, and Shabel’skoe profiles) on the Terek-Kuma Plain and the Azov-Kuban’ Lowland was performed. An increase in the concentrations of Fe and Mn, which are intensively involved in the biological cycle, and Rb, which is accumulated due to the activation of weathering processes, was observed in the paleosols that developed in interglacial periods of activation of pedogenesis. Increased coefficients of weathering (chemical index of alteration (CIA)) = [Al/(Al + Ca + Na + K)] 100, Al/(Al + Ca + Na + Mg), Rb/Sr, and Mn/Sr), leaching (Ba/Sr), and biological activity and bioproductivity (Mn/Fe, Mn/Al) were also noted for the paleosol horizons as compared with the loess horizons. It is argued that geochemical coefficients can be used as an efficient tool in the soil and paleogeographic studies aimed at the reconstruction and refinement of the schemes of changes in the bioclimatic conditions during the Pleistocene.  相似文献   

9.
The transition from hunting gathering to a farming based economy – the Neolithic Revolution, was a crucial junction in the human career, attracting the attention of many scholars: archaeologists, anthropologists, geographers, botanists, geneticists and evolutionists among others. Our understanding of this major transformation is rather limited mainly due to the inability to fully reconstruct the cultural, biological and environmental setup of the relevant period and organisms involved. Many students of the subject of plant domestication have seriously entertained the hypothesis that man's first crop plants have originated from weeds associated with the disturbed habitats surrounding pre-agricultural ancient human dwellings and or with human refuse heaps – the so called ‘dump heap hypothesis’. In this paper we re-examine this hypothesis in light of the known biology of the Near Eastern founder crops and the ecological preferences of their wild progenitors. Contrary to the ‘dump-heap hypothesis’, we propose that Near Eastern farming originated as a result of a long term interaction between humans and plants and was mainly driven by the nutritional features of the respective crops and cultural forces.  相似文献   

10.
Protected areas are considered fundamental to the preservation of nature. The International Union for Conservation of Nature (IUCN) has standardized categories for protected areas designation, which are intended to represent varying levels of regulatory protection. We evaluate whether the present assignment of protected areas to IUCN categories corresponds to the expected gradient of naturalness in a globally consistent manner. Our proxy of naturalness was a global map of human influence known as Human Footprint (HF). Higher HF values represent less intact natural areas. Our final sample of protected areas included 21,186 IUCN-designated sites that were ?1 km2. We used multiple linear regression to test for the effect of IUCN categories on mean HF while accounting for biome and protected area size.The present assignment of protected areas to IUCN categories does not correspond to the expected gradient of naturalness. We observed that IUCN Category Ia areas have higher HF than expected and Category VI protected areas have unexpectedly low HF; Category VI protected areas also are generally larger than protected areas of other categories. Reporting of area protected in different IUCN categories is one measure of progress towards meeting commitments under the Convention on Biological Diversity, yet because IUCN categories are not interpreted consistently across the globe, this is not a reliable metric. Further, despite the social, economic and ecological importance of protected areas with a very low HF, our results suggest that the availability of such areas within the global protected areas network is very limited.  相似文献   

11.
Few data are available about the effects of complexation of polyphenols with polysaccharide on their bioavailability. The complex of tea polyphenols (TP) with oat β-glucan was characterized by ultraviolet-visible spectrometry, Fourier transform infrared spectrometry, differential scanning calorimetry, atomic force microscopy, and solid-state (13)C NMR spectroscopy. The results indicated that the bonds which governed the interaction between TP and oat β-glucan were strong hydrogen bonds. The in vitro antioxidant activity of TP, β-glucan, their complex, and physical mixture was assessed using four systems, namely, DPPH(?), OH(?), and O(2)(?-) scavenging activities and reducing power. The complexation and blending of TP and β-glucan exhibited different impacts on the index of in vitro and in vivo antioxidant capacities. In the concentration range of 0.5-2.5 mg mL(-1), the complex had highest O(2)(?-) scavenging activity, whereas the highest OH(?) scavenging activity was found with the physical mixture. For antioxidant testing in vivo, there was no significant difference between the complex and the physical mixture in terms of glutathione peroxidase activity and levels of malondialdehyde and total antioxidant capacity in serums. However, the complex exhibited much higher activities of superoxide dismutase and glutathione peroxidase in livers than the physical mixture. The present study provided a deeper understanding of the influence of molecular interaction between TP and oat β-glucan on their antioxidant activities.  相似文献   

12.
The cold tolerance of flower buds of apple Malus domestica Borkh. ('Mclntosh'/M.7) declined during spring growth. The absence of fruit in the previous year increased the ability of the buds to withstand lower temperatures. All flower buds retained the capacity to tolerate freezing injury until first pink stage. The median lethal temperature (MLT) of the flower buds increased relatively throughout the sampling period. The flower buds of cropped trees were more susceptible to low temperature injury during spring at all stages of development. The flower buds of non‐cropped trees contained higher levels of hydrophilic and acidic amino acids from October until April, which may be related to their greater cold tolerance. A new nitrogen:protein conversion factor (5.51) was calculated based on total amino acid analysis which could be substituted for 6.25 as a means of estimating total protein content of apple flower bud tissue using the Kjeldahl method.  相似文献   

13.
The topsoils of two sites, comprising natural forest and 4- and 20-year-old pastures, respectively, were selected in Rondônia to evaluate the changes of soil organic matter due to pasture establishment. These changes were evaluated by measuring the proportions of the C and N associated with clay and silt fractions, and by the C decomposition (CD) rate of the whole topsoils and their size fractions. The topsoils studied had large proportions of C and N associated with fine fractions, especially with clay fractions. The CD rate of the silt fractions was higher than that of the clay fractions under the two forest topsoils and under the 20-year-old pasture. The CD rate of the silt fractions under forest vegetation at each site was significantly higher than that of the silt fractions under pasture vegetation at the same site. The CD of clay fractions followed the same trend as the silt fractions, showing an improvement in the stability of C associated with clay and silt fractions under pasture vegetation.  相似文献   

14.

Purpose

This work explores the application of the use of Zn, Cu, and Pb relative contents as a new type of normalization method for geochemical properties of soils and sediments in an Atlantic Basin (Anllóns River, NW Spain). The method is based on the conservative behavior of these elements, which exhibit a certain concentration ratio that remains stable as long as there are no human disturbances.

Materials and methods

The average relative contents of Zn, Cu, and Pb were calculated by dividing the concentration of each metal in soils or sediments, in the <63-μm fraction, by the sum of Zn, Cu, and Pb, expressed as a percentage. The evaluation of the sum of the average relative concentrations of Zn, Cu, and Pb (Ri), together with three standard deviations for each element, namely, Ri ± 3Si, allows a hexagon to be constructed, represented in ternary diagrams of Zn:Cu:Pb. Following the method proposed by Weng et al. (Environ Geol 45:79–85, 2003), those samples falling outside the hexagon must be considered outliers.

Results and discussion

Results obtained confimed the conservative behavior between the relative contents of Zn, Cu, and Pb in surficial samples (soils, bed, and suspended sediments). Only sediment cores displayed nonconservative behavior, showing a marked Pb enrichment, with respect to the surficial samples. When Zn, Cu, and Pb relative contents were plotted in ternary diagrams, outliers were best classified when the hexagon was drawn with standard deviations of samples from the study area. The hexagon drawn with an international database of soils and sediments showed a poorer classification of outliers.

Conclusions

The results showed that total Zn, Cu, and Pb relative contents may be employed to investigate anthropogenic disturbances of these elements in soils and sediments of the Anllóns River Basin, thus corroborating that this type of normalization may be employed as a tool to assess outliers in a contaminated area.  相似文献   

15.
Abstract

Micronutrients may be provided to plants in containers either via pre‐plant inclusion in the growing medium or via fertigation, or both. For Hebe ’Inspiration’ plants growing in a soilless potting medium, micronutrients applied as a single pre‐plant application or via weekly liquid fertilizer applications were equally effective in producing optimum growth over a 12‐month period. Under the conditions of this experiment, the liquid fertilizer did not need to contain more than (in mg/L) 0.5 = iron (Fe), 0.1 = copper (Cu), 0.1 = zinc (Zn), 0.8 = manganese (Mn), and 0.1 = boron (B). Growth was excellent for the full 12 months, without supplementation via the liquid fertilizer, when there were micronutrient concentrations of (in mg/L extract) 29 = Fe, 0.5 = Cu, 3 = Mn, 0.6 = Zn, and 0.14 = B in a 1:1.5 volume in a 2mM DTPA extract of the medium at potting.  相似文献   

16.
Purpose

The aim of this work was to study the level and degree of mobility of heavy metals in the soil–plant system and to perform bioindication observations in the Don River estuarine region and the Russian sector of the Taganrog Bay coast.

Materials and methods

The objects of the study included samples of zonal soils (chernozem) and intrazonal soils (alluvial meadow and alluvial-stratified soils, Solonchak, sandy primitive soil) from monitoring stations of the Don river estuarine region and the Taganrog Bay coast, as well as their higher plants: Phragmites australis Cav., Typha angustifolia L., Carex riparia Curtis, Cichorium intybus L., Bolboschoenus maritimus L. Palla, and Rumex confertus Willd. The total concentrations of Mn, Ni, Cd, Cu, Zn, Pb, and Cr in the soils were determined by X-ray fluorescent scanning spectrometer. The concentration of heavy metal mobile forms exchangeable, complex compounds, and acid-soluble metal were extracted using the following reagents: 1 N NH4Ac, pH 4.8; 1 % EDTA in NH4Ac, pH 4.8; 1 N HCl, respectively. Heavy metals in plants were prepared for analysis by dry combustion at 450 °C. The heavy metal concentration in extracts from plants and soils was determined by AAS.

Results and discussion

The total contents of heavy metals in the soil may be described with a successively decreasing series: Mn?>?Cr?>?Zn?>?Ni?>?Cu?>?Pb?>?As?>?Cd. The total concentrations of As, Cd, and Zn in the soil exceed the maximum permissible concentrations levels. Contamination of alluvial soils in the estuarine zone with mobile Сu, Zn, Pb, and Cd has been revealed, which is confirmed by the high bioavailability of Cu and Zn and, to a lesser degree, Cd and Pb accumulating in the tissues of macrophytic plants. Data on the translocation of elements to plant organs have showed their predominant accumulation in the roots. Bioindication by the morphofunctional parameters of macrophytic plants (with a Typha L. species as an example) can be used for revealing the existence of impact zones with elevated contents of metals in aquatic ecosystems.

Conclusions

The results revealed that increased content of Zn, Pb, Cu, Ni, and As in soil have anthropogenic sources. The high content of Cr in the soils is related to the lithogenic factor and, hence, has a natural source.

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17.
The Norfolk Broads and rivers of eastern England (Fig. 1) comprise an area hitherto farmed for the diversity of its wildlife and submerged aquatic plant communities. The latter have progressively disappeared since the early 1950s, until only four sites currently retain more than remnants of the original sub-aquatic macrophyte flora and its associated invertebrate fauna. Increases in turbidity of the water have been associated with the loss of macrophytes, and these increase have been variously attributed to phytoplankton and to disturbance of sediment by the many boats of visiting tourist and residents. Synoptic surveys of turbidity were carried out in the navigable waterways of Broadland in summer and winter 1973, and of phytoplankton in summer 1973. The differential distribution of phytoplankton is discussed in terms of the nutrient loadings on, and flushing coefficients of, the waterway. Highly significant correlations were obtained between phytoplankton numbers and turbidity in the system as a whole and Broads and rivers considered separately. A very weak correlation between boat activity and turbidity was shown to be non-causative. It is concluded that increase in turbidity is a function of increased nutrient loading from human activities in the catchment area and that boat disturbance does not contribute significantly to the sustained turbidity.  相似文献   

18.
A methodological approach to the physically sound mathematical simulation of the hydrothermal regime for a complex soil cover on an agricultural field scale was proposed. To realize the approach, it is necessary (1) to restore the hydrothermal regime of two contrasting soils using a physically sound mathematical model on the basis of the experimental thermophysical and hydrophysical characteristics, (2) to adapt and optimize the model using the available experimental regime data, (3) to determine the hydrophysical and thermophysical properties for the entire plot under study using pedotransfer functions, and (4) to restore the hydrothermal regime of the entire soil plot using a mathematical model and the meteorological data for a specific time period. The proposed procedure allows simulating the hydrothermal regime of an agrogray soil complex in the Vladimir Opol??e region with a normalized standard error of about 8%. The analysis of the hydrothermal regime for the soil cover of the studied plot calculated from the meteorological data for the period from May to August of 2009 showed that the lower temperature values were confined to the areas of the agrogray soils with the second humus horizon: their average temperature was lower than the temperature of the agrogray soils by 0.44, 0.93, and 1.32°C at depths of 20, 40, and 70 cm, respectively, and the differences between their sums of the active temperatures for the considered period of 2009 reached 89 and 74°C at depths of 20 and 40 cm, respectively.  相似文献   

19.
Journal of Soils and Sediments - The application of a large amount of inorganic nitrogen (N) fertilizer resulted in an increasing N loss. It is an effective practice that biochar and organic...  相似文献   

20.
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