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1.
  【目的】  依托位于江西红壤研究所的有机肥长期定位试验,探究长期施用紫云英、猪粪和秸秆还田对土壤微生物量、酶活性和产量的影响,阐明土壤微生物学特性对土壤肥力的生物指示功能,揭示影响作物产量和微生物学特性的关键环境因子。  【方法】  长期定位试验始于1981年,选取的6个处理分别为:CK (不施肥)、NPK (单施化肥)、M1 (早稻施绿肥)、M2 (早稻加倍施绿肥)、M3 (早稻施绿肥+晚稻施猪粪) 和M4 (早稻施绿肥+晚稻秸秆还田),此外,有机处理 (M1、M2、M3和M4) 在施用有机物料的基础上,每季还补充一定量化肥(N 69 kg/hm2、P2O5 30 kg/hm2、K2O 67.5 kg/hm2)。于每季收获后测定水稻产量。2018 年早稻收获后,采集耕层 (0—20 cm)土壤,测定土壤化学指标、微生物量碳氮、脲酶及其他参与土壤碳、氮、磷和硫循环的胞外酶活性。  【结果】  1) 2009—2018年间,长期施肥处理明显提高了水稻产量,其中M3处理的产量最高,其次为M2处理,二者均明显高于NPK处理;2) 土壤微生物量及酶活性均以有机处理 (M1、M2、M3和M4) 较高,其中M3处理土壤微生物量碳含量及各类土壤酶活性最高,M2处理土壤微生物量氮含量最高。有机处理较CK和NPK处理提高了土壤微生物熵。此外,微生物量碳氮和土壤酶活性与有机碳、全氮间存在显著或极显著相关关系;3) 有机碳与土壤微生物量 (0.895)、碳循环相关酶活性 (0.903)、氮循环相关酶活性 (0.854) 及水稻产量 (0.827) 呈显著正影响 (P < 0.05);土壤pH与土壤碳循环相关酶活性 (0.378) 和氮循环相关酶活性 (0.365) 呈显著正影响 (P < 0.05),对水稻产量 (0.211) 也表现为正影响,但不显著 (P > 0.05)。相较于pH,土壤有机碳含量在提高水稻产量、改善土壤微生物学特性上发挥了更为重要的作用。  【结论】  土壤微生物量碳氮和参与土壤碳、氮、磷和硫循环的胞外酶活性均可作为土壤微生物学指标表征土壤肥力的变化。土壤有机碳含量是提高作物产量、调控土壤微生物量和酶活性的关键因子。在供试条件下,长期实行早稻施绿肥+晚稻施猪粪的有机培肥,是提高土壤微生物量及酶活性并提高水稻产量的最佳选择。  相似文献   

2.
根际土壤有机氮组分在土壤养分和作物氮素营养中具有重要作用。本研究依托长期(37年)定位施肥试验田,设置4个施肥处理:不施肥对照(CK)、单独施用化肥(CF)、秸秆还田+化肥(RF)和30%有机肥+70%化肥(OM),于晚稻成熟期测定大麦-双季稻田根际土壤基础理化性质、微生物量氮和有机氮组分(氨基酸态氮、氨基糖态氮、酸解氨态氮、酸解未知态氮、非酸解性氮)含量。研究表明:相对CK处理,RF和OM处理显著增加了稻田根际土壤有机碳、全氮、铵态氮和硝态氮的含量。RF和OM处理土壤微生物量氮含量分别比CK处理增加了19.8%和30.7%。酸解性氮作为根际土壤全氮的主体部分,占全氮的59.6%~72.1%;各处理根际土壤酸解性氮含量大小顺序表现为OM>RF>CF>CK。各施肥处理中,酸解有机氮中的氨基糖态氮、氨基酸态氮和酸解未知态氮含量均以OM处理最大,分别比CK处理增加139.3%、47.9%和110.0%;酸解氨态氮以RF处理最大,比CK处理增加69.9%。土壤有机碳、全氮、铵态氮、硝态氮与土壤氨基酸态氮、氨基糖态氮、酸解未知态氮以及微生物量氮均呈极显著(p<0.01)正相关。因此,秸秆、有机肥配施化肥均能有效提高大麦-双季稻田根际土壤的供氮能力,是改善稻田土壤肥力的有效手段。  相似文献   

3.
有机物料对原生盐碱地土壤生物学性质的影响   总被引:5,自引:0,他引:5  
何瑞成  吴景贵 《土壤学报》2018,55(3):774-782
采用连续2年大田试验方法,研究了颗粒型秸秆(KL)、正常秸秆(JG)、牧草(MC)以及羊粪(YF)4种有机物料处理对试验地微生物数量、微生物生物量碳氮、土壤呼吸强度、三种土壤酶活性以及水稻产量的影响,并分析了生物学性质与测产指标的相关性。结果表明:与不施有机物料处理(CK)对比,施用有机物料的JG、MC、YF处理均不同程度提高了微生物数量、微生物生物量碳氮含量、土壤呼吸强度、土壤酶活性以及水稻产量,但差异不明显。同种有机物料不同形态之间差异显著,颗粒秸秆效果优于正常秸秆,颗粒秸秆与CK相比,提高了39.45%的土壤细菌、50.28%放线菌、89.91%真菌数量、63.21%和46.02%的土壤微生物生物量碳氮含量,土壤呼吸强度提高46.22%,过氧化氢酶活性提高18.03%,转化酶活性提高23.22%,纤维素酶活性提高79.32%,增产130.6%。土壤微生物数量(细菌、真菌和放线菌)与千粒重、结实率、每穗总粒数和产量具有相关性,土壤微生物活性(土壤呼吸强度和微生物生物量碳氮)与有效穗数、每穗总粒数、千粒重以及产量具有相关性,土壤酶活性(过氧化氢酶、转化酶和纤维素酶)与穗长和千粒重表现出相关性。结果表明相同条件下,原生盐碱地改良前期,不同种类有机物料对原生盐碱地作用效果相似,但同种有机物料不同形态其作用效果差异显著。  相似文献   

4.
Amino sugars, as a microbial residue biomarker, are highly involved in microbial-mediated soil organic matter formation. However, accumulation of microbial biomass and responses of bacterial and fungal residues to the management practices are different and poorly characterized in rice soils. The objectives of this study were to evaluate the effects of mineral fertiliser (MIN), farmyard manure (FYM) and groundnut oil cake (GOC) on crop yield and co-accumulation of microbial residues and microbial biomass under rice-monoculture (RRR) and rice–legume–rice (RLR) systems. In the organic fertiliser treatments and RLR, rice grain yield and stocks of soil and microbial nutrients were significantly higher than those of the MIN treatment and RRR, respectively. The increased presence of saprotrophic fungi in the organic fertiliser treatments and RRR was indicated by significantly increased ergosterol/Cmic ratio and extractable sulphur. In both crop rotation systems, the long-term application of FYM and GOC led to increased bacterial residues as indicated by greater accumulation of muramic acid. In contrast, the higher fungal C/bacterial C ratio and lower ergosterol/Cmic ratio in the MIN treatment, is likely caused by a shift within the fungal community structure towards ergosterol-free arbuscular mycorrhizal fungi (AMF). The organic fertiliser treatments contributed 22 % more microbial residual C to soil organic C compared to the MIN treatment. Our results suggest that the negative relationship between the ratios ergosterol/Cmic and fungal C/bacterial C encourages studying responses of both saprotrophic fungi and AMF when assessing management effects on the soil microbial community.  相似文献   

5.
采用盆栽试验研究了蔬菜废弃物堆肥对小白菜的增产效果、土壤养分含量、土壤微生物量和酶活性的影响。结果表明,蔬菜废弃物堆肥能够显著提高盆栽小白菜的产量和品质,其中30 t/hm2的高量蔬菜废弃物堆肥将产量提高了66.26%,将品质指标Vc、可溶性糖和可溶性蛋白含量显著提高了35.64%、183.36%和39.42%。蔬菜废弃物堆肥能够显著提高土壤质量,有机质、总氮、碱解氮、有效磷、速效钾含量和土壤微生物量碳、氮,以及土壤淀粉酶、脲酶、磷酸酶、脱氢酶活性的土壤质量指标。与牛粪相比较,高用量的蔬菜废弃物堆肥处理在小白菜产量、可溶性蛋白含量、土壤碱解氮、有效磷、速效钾含量、磷酸酶活性上显著高于牛粪;而在可溶性糖含量、土壤有机质含量、土壤微生物量、淀粉酶、脱氢酶活性上显著低于牛粪。综合而言,蔬菜废弃物堆肥能够提高土壤质量,增加蔬菜产量和品质;在蔬菜产量方面,蔬菜废弃物堆肥优于牛粪,在蔬菜品质和土壤质量方面,蔬菜废弃物堆肥与牛粪相当。  相似文献   

6.
The addition of organic matter via green manure rotation with rice is considered a smart agricultural practice to maintain soil productivity and support environmental sustainability. However, few studies have quantitatively assessed the impact of green manure rotation and application on the interactions between agronomic management practice, soil fertility, and crop production. In this study, 800 pairs of data from 108 studies conducted in the agricultural region of the Yangtze River, China were...  相似文献   

7.
有机无机配施对水稻产量及氮肥残效的影响   总被引:1,自引:0,他引:1       下载免费PDF全文
为揭示有机无机配施条件下15N尿素在稻田土壤中的残效状况,在我国北方棕壤性水稻土上进行盆栽试验,结合土壤氮素供应及水稻对肥料氮的吸收利用,探寻最佳的施肥方式.设置空白(CK)、尿素(N)、秸秆+尿素(NS)、猪粪+尿素(NM)、稳定性尿素(NI)、秸秆+稳定性尿素(NIS)、猪粪+稳定性尿素(NIM)7个处理.结果 表...  相似文献   

8.
Substituting chemical fertilizers with manure is an important method for efficient nutrient management in rice cropping systems of China.Labile nitrogen(N) is the most active component of the soil N pool and plays an essential role in soil fertility.However,the effects of manure substitution on soil labile N in rice cropping systems and their relationships with soil properties,fertilization practices,and climatic conditions remain unclear and should be systematically quantified.Here,we investiga...  相似文献   

9.
Soils receiving organic manures with and without chemical fertilizers for the last 7 yr with pearlmillet–wheat cropping sequence were compared for soil chemical and biological properties. The application of farmyard manure, poultry manure, and sugarcane filter cake alone or in combination with chemical fertilizers improved the soil organic C, total N, P, and K status. The increase in soil microbial‐biomass C and N was observed in soils receiving organic manures only or with the combined application of organic manures and chemical fertilizers compared to soils receiving chemical fertilizers only. Basal and glucose‐induced respiration, potentially mineralizable N, and arginine ammonification were higher in soils amended with organic manures with or without chemical fertilizers, indicating that more active microflora is associated with organic and integrated system using organic manures and chemical fertilizers together which is important for nutrient cycling.  相似文献   

10.
【目的】 施肥能直接或间接改变农田生态系统的养分平衡,从而影响土壤的物理、化学和生物学特性。本研究探讨不同种植制度和土壤条件下施肥对农田土壤生物学特性的影响程度,为合理施肥和土壤肥力提升提供科学依据。 【方法】 通过收集近10年 (2008—2018年) 来发表的文献,建立了包含185组微生物量及群落结构等相关内容的数据库。采用整合分析方法(Meta-analysis),定量分析了施肥对土壤微生物量、群落结构以及酶活性的影响。 【结果】 与不施肥相比,施肥显著提高了土壤微生物磷脂脂肪酸 (PLFA) 和微生物量碳、氮含量,提高幅度分别为28.5%、30.9%和41.6%。施用 (单施或配施) 有机物料对土壤微生物总PLFA含量及微生物量碳、氮含量的提高幅度分别为47.3%、50.4%和58.7%,相当于施用化肥的2.8、2.4和3.9倍。与不施肥相比,施肥均能增加各类微生物菌群PLFA含量,对细菌、真菌及放线菌的提高幅度为23.8%~30.4%,对革兰氏阴性菌(G–)和革兰氏阳性菌 (G+) 的提高幅度为37.8%~43.2%,且施用有机物料处理对各类微生物菌群PLFA含量的提高幅度显著高于施化肥处理。施用化肥对土壤微生物总PLFA含量的提高幅度在一年两熟制区为17.9%,在水田和水旱轮作条件下为18.3%~27.6%,而在一年一熟制区及旱地条件下对土壤微生物总PLFA含量无显著影响。在不同pH的土壤中,施用有机物料对微生物总PLFA的提高幅度均显著高于施化肥处理。在pH < 6与pH > 8的土壤上施用化肥对微生物总PLFA含量无明显影响。施肥显著提高了与土壤有机质分解相关的β-葡萄糖苷酶(42.4%)和乙酰氨基葡萄糖苷酶(174.5%)的活性,对与氮循环相关的亮氨酸氨基肽酶活性无显著影响。统计分析还表明,施肥并未改变土壤微生物的真菌细菌比(F∶B)和革兰氏阳性菌革兰氏阴性菌比(G +∶G–)。 【结论】 在不同种植制度、土地利用类型和土壤pH下,施肥显著改变了土壤微生物量和与有机质分解相关的酶活性,但未改变土壤微生物的真菌细菌比(F∶B)和革兰氏阳性菌革兰氏阴性菌比(G+∶G–)。单施或配施有机物料均有利于提高农田土壤微生物总量及各类菌群的生物量,效果显著好于单施化肥。   相似文献   

11.
Evaluation of biofuel production cropping systems should address not only energy yields but also the impacts on soil attributes. In this study, forage sorghum (Sorghum bicolor L. Moench) cropping systems were initiated on a low organic matter soil (<0.9 %) with a history of intensively tilled low-input cotton production in the semiarid Southern High Plains of the U.S. Sorghum cropping systems were evaluated in a split-plot design with sorghum cultivar as the main plot and the combination of irrigation level (non-irrigated and deficit irrigated) and aboveground biomass removal rate (50 % and 100 %) as the split plot. The sorghum cultivars used varied in yield potential and lignin content, which are important features for feedstock-producing crops. Within 1 year, the transition from long-term cotton cropping systems to sorghum biofuel cropping systems resulted in increased soil microbial biomass C (16 %) and N (17 %) and shifts in the microbial community composition as indicated by differences in fatty acid methyl ester (FAME) profiles. Additionally, enzyme activities targeting C, N, P and S cycles increased 15–75 % (depending on the enzyme) after two growing seasons. Increased enzyme activities (16–19 %) and differences in FAME profiles were seen due to irrigation regardless of aboveground biomass removal rate. Biomass removal rate and the cultivar type had little effect on the soil microbial properties during the time frame of this study. Early results from this study suggest improvements in soil quality and the sustainability of sorghum biofuel cropping for low organic matter agricultural soils.  相似文献   

12.
 Soil organic matter level, mineralizable C and N, microbial biomass C and dehydrogenase, urease and alkaline phosphatase activities were studied in soils from a field experiment under a pearl millet-wheat cropping sequence receiving inorganic fertilizers and a combination of inorganic fertilizers and organic amendments for the last 11 years. The amounts of soil organic matter and mineralizable C and N increased with the application of inorganic fertilizers. However, there were greater increases of these parameters when farmyard manure, wheat straw or Sesbania bispinosa green manure was applied along with inorganic fertilizers. Microbial biomass C increased from 147 mg kg–1 soil in unfertilized soil to 423 mg kg–1 soil in soil amended with wheat straw and inorganic fertilizers. The urease and alkaline phosphatase activities of soils increased significantly with a combination of inorganic fertilizers and organic amendments. The results indicate that soil organic matter level and soil microbial activities, vital for the nutrient turnover and long-term productivity of the soil, are enhanced by use of organic amendments along with inorganic fertilizers. Received: 6 May 1998  相似文献   

13.
 In arable soils in Schleswig-Holstein (Northwest Germany) nearly 30% of the total organic C (TOC) stored in former times in the soil has been mineralized in the last 20 years. Microbial biomass, enzyme activities and the soil organic matter (SOM) composition were investigated in order to elucidate if a low TOC level affects microbial parameters, SOM quality and crop yield. Microbial biomass C (Cmic) and enzyme activities decreased in soils with a low TOC level compared to soils with a typical TOC level. The decrease in the Cmic/TOC ratio suggested low-level, steady-state microbial activity. The SOM quality changed with respect to an enrichment of initial litter compounds in the top soil layers with a low TOC level. Recent management of the soils had not maintained a desirable level of humic compounds. However, we found no significant decrease in crop yield. We suggest that microbial biomass and dehydrogenase and alkaline phosphatase activities are not necessarily indicators of soil fertility in soils with a high fertilization level without forage production and manure application. Received: 12 December 1997  相似文献   

14.
生物有机肥提高设施土壤生产力减缓黄瓜连作障碍的机制   总被引:9,自引:0,他引:9  
【目的】针对土壤连作障碍问题,探索高强度连作条件下生物有机肥在减缓土壤连作障碍发生、平衡土壤养分、调控土壤酶活性等方面的作用机制,为生物有机肥在改良土壤、防控黄瓜连作障碍、促进农业可持续发展等方面的应用奠定基础。【方法】以如皋市农业科学研究所设施蔬菜种植基地为试验平台,采用田间定位试验的方法,设置CK (空白对照)、B10 (生物有机肥施用量为10 t/hm2)、B20 (生物有机肥施用量为20 t/hm2) 3个处理,于第一季黄瓜种植前将生物有机肥一次性施入试验小区,于2016年12月5日、2017年6月28日和2017年11月26日,连续三季,在黄瓜成熟采集0—20 cm土壤样品。将土壤样品分成两份,一份放于4℃冰箱冷藏保存,用于测定土壤生物学性质;另一份风干后过筛处理,以供土壤理化性状分析。同时,调查黄瓜产量。【结果】随着黄瓜连续种植季数增加,CK处理中土壤容重和电导率呈增加趋势,土壤各肥力指标、酶活性、微生物量碳氮含量及黄瓜产量呈下降趋势;在同一季黄瓜成熟期,与CK相比,施用生物有机肥可降低土壤容重和电导率,增加土壤总孔隙度、pH、有机质、全氮、硝态氮和速效磷含量,其中连续种植3季黄瓜过程中,土壤有机质、速效磷和硝态氮含量分别增加了39.3%~83.9%、5.98~14.2%和10.0~22.0%,而土壤电导率降低了8.57%~12.7%。同一季黄瓜成熟期,不同处理中土壤脲酶、过氧化氢酶、蔗糖酶和磷酸酶活性由大到小排列均表现为B20 > B10 > CK,且连续种植3季黄瓜后B10和B20处理中4种土壤酶活性均高于第一季黄瓜成熟期CK处理;连续种植3季黄瓜期间,施用生物有机肥处理中土壤微生物量碳、氮含量均明显高于CK,B10和B20处理中微生物量碳、氮含量较CK分别增加了4.26%~23.2%和21.8%~56.2%。与CK相比,第一季、第二季、第三季B10处理黄瓜产量分别提高了3.93%、9.73%、10.6%,B20处理分别提高了12.9%、18.8%、20.2%。黄瓜连作过程中,土壤容重、有机质、硝态氮和速效磷与生物学指标和黄瓜产量相关显著,且土壤pH和电导率与土壤过氧化氢酶活性、蔗糖酶活性、微生物量氮含量和黄瓜产量呈显著相关关系。【结论】生物有机肥可改善黄瓜连作过程中土壤耕性变差、养分失衡和酸化等理化性质变劣的现象,缓解黄瓜连作土壤的次生盐渍化状况,还能提高土壤酶活性、微生物量碳氮含量和黄瓜产量,且黄瓜连作过程中土壤理化性质和生物学特性指标无明显变化,从而减缓黄瓜连作障碍的发生。  相似文献   

15.
Soil in short-term crop rotation systems (STCR) is still in the initial development stage of farmland soil, whereas after long-term crop rotation treatment (LTCR), soil properties are significantly different. This study compares STCR (4 years) and LTCR (30 years) rice-rice-fallow, rice-rice-rape rotation practices under the same soil type background and management system. To reveal ecosystem mechanisms within soils and their effects on rice yield following LTCR, we analyzed the physical, chemical, and microbiological properties of soils with different rotations and rotation times. Relative to STCR, LTCR significantly reduced soil water-stable aggregate (WSA) content in the <?0.053-mm range, while >?2 mm WSA content significantly increased. Soil organic matter increased in fields under LTCR, mainly in >?2 mm, 2–0.25 mm, and <?0.053 mm soil WSA in 0–10 cm soil layer. LTCR was associated with significantly increased total soil organic matter, at the same time being associated with increasing the amount of active organic matter in the 0–20 cm soil layer. The two crop rotation regimes significantly differed in soil aggregate composition as well as in soil N and P, microbial biomass, and community composition. Relative to STCR, LTCR field soils had significantly higher soil organic matter, active organic matter content, soil enzyme activities, and overall microbial biomass, while soil WSA and microbial community composition was significantly different. Our results demonstrate that LTCR could significantly improve soil quality and rice yield and suggest that length of rotation time and rice-rice-rape rotation are critical factors for the development of green agriculture.  相似文献   

16.
A field experiment was conducted on summer mungbean residue recycling (SMBRR) and basmati rice–wheat cropping system (BRWCS) at New Delhi, India. The SMBRR enhanced the system productivity and net returns by ~19.1% and 22.1% compared to summer fallow (SF) with highest magnitude under genotypic sequence of P 2511/HD 2967. Two genotypes each in basmati rice (PB 1 and P 2511) and wheat (HD 2967 and HD 2733) responded well to SMBRR with respect to grain yield efficiency index (GYEI) ≥ 1.0. SMBRR also registered ~13.5% higher microbial biomass carbon (MBC) than SF. Soil organic carbon (SOC) storage also increased by ~6.8% in 0–30 cm soil layer. The rice–wheat–summer mungbean system produced significantly highest energy efficiency compared to the rice–wheat–summer fallow system with highest values under genotypic sequence of P 2511/HD 2967 as a result of better yield expression. Overall, SMBRR with suitable genotypic sequence improved the system productivity, profitability, and nutrient dynamics in BRWCS, which are vital for long-term sustainability of this system.  相似文献   

17.
研究了添加有机、无机营养物质对外加除草剂莠去津土壤(每1g土中含莠去津10mg/)中微生物生物量碳、氮和磷的动态变化过程。研究结果表明,在整个培养过程中,仅加莠去津的土壤中微生物生物量碳、氮、磷的含量均显著降低,与对照相比,分别平均降低了13.5%,10.1%,20.0%。但是,施用有机、无机营养物质的处理,土壤微生物生物量碳、氮、磷的含量均显著增加。不同处理对微生物生物量碳、氮的含量影响程度依次为:腐熟猪粪>紫云英>水稻秸秆腐熟猪粪>N、P肥配施>单施N肥>单施P肥。而对微生物生物量磷含量的影响则为:腐熟猪粪>N、P肥配施>紫云英>单施N肥>单施P肥>水稻秸秆。  相似文献   

18.
We have studied the influence of long-term fertilization on organic C, nutrients, microbial biomass of soil, and grain yield of rice (Oryza sativa L.) after 18 years of inorganic and organic fertilizer application to an infertile paddy field under subtropical conditions. After 18 years of fertilization, soil organic C and total N contents were 9.56~12.17 and 1.01~1.25 g kg−1, which were 190~269% and 135~191% higher than those of the initial soil, respectively. Organic manure application increased soil organic C, total N, available N, and available P contents by 19.2%, 14.4%, 13.2%, and 78.3% on average compared with organic manure-omitted treatments. Phosphorus fertilizer application increased soil available P content more significantly than organic manure application, and it was an average 385% higher than P-omitted treatments. Soil microbial biomass C, N, and rice yield were 48.9%, 33.2%, and 133% higher in organic manure application treatments and 36.8%, 38.8%, and 239% higher in P fertilizer application treatments than organic manure-omitted or P-omitted treatments, respectively. Incorporation of organic manure-enhanced and P fertilizer-enhanced rice yields by 382% compared with the unfertilized treatment and yield increment index was 123% compared with that observed during 1991–1994, implying that organic manure application combined with P fertilizer was needed to ensure high and sustainable productivity. Rice yield was significantly correlated with all soil chemical properties except available K content, and stepwise regression analysis showed that soil available P content was the limiting factor to rice yield.  相似文献   

19.
Removing solids from liquid dairy manure slurry reduces manure phosphorus (P) and increases the available (mineral) fraction of nitrogen (N) but also decreases the organic matter content of the manure. While this novel treatment reduces environmental concerns associated with excess N and P application to soils, it may also reduce microbial biomass and activity in soil. This study evaluated the long-term effects of this alternative manure treatment compared to more typical nutrient applications in a perennial grass sward (tall fescue, Festuca arundinacea Schreb.) on soil microbial biomass, community composition, hydrolytic enzyme activity, and forage yield. Nutrient treatments for this long-term field experiment in Agassiz, British Columbia, Canada were started in 2003. The treatments included liquid dairy manure slurry, liquid dairy manure with solids removed, commercial fertilizer, a combination of fertilizer and dairy manure, and a control. All treatments were applied at 400–600 kg total N ha?1 year?1 in four equal doses. Soil microbial community composition (phospholipid fatty acid analysis) and activity (hydrolytic enzyme activity) were determined several times during the 2013 and 2014 growing seasons to a depth of 15 cm. Time of sampling (date) had a strong influence on microbial biomass, community composition, and activity, while the response to soil properties and yield was more varied. All manure treatments (dairy manure slurry, liquid fraction, and the combination) increased microbial biomass (by 19–32%) and the potential activity of cellulose-degrading enzymes (by 31–47%) compared to commercial fertilizer and unamended plots. The commercial fertilizer and liquid fraction lowered fungal/bacterial ratios compared with both whole manure and unamended plots. Our results indicate that separating the solid from the liquid fraction of manure, to improve crop yield and reduce P loading, did not reduce microbial community size and activity and that all manure treatments increased microbial biomass and activity compared to mineral fertilizer application.  相似文献   

20.
稻草高茬-紫云英联合还田改善土壤肥力提高作物产量   总被引:20,自引:4,他引:20  
研究旨在探讨稻草留高茬套种绿肥、稻草-绿肥联合还田下的生产及土壤肥力特征,为南方稻区综合利用稻草和绿肥提供理论及技术支撑。2012—2016年设置定位试验,研究高茬稻草-绿肥联合还田下的绿肥和水稻产量、土壤碳氮库活性及其他养分特征。试验包括5个处理:冬闲+稻草不还田(CK),冬闲+稻草全量还田(RS),冬种紫云英+稻草不还田(MV),冬种紫云英+稻草低茬全量还田(MV+LRS),冬种紫云英+稻草高茬全量还田(MV+HRS),各处理施用等量化肥。结果表明:稻草-绿肥联合还田提高绿肥产草量及其含氮量,与MV相比,分别增加了13.1%和6.8%(MV+LRS)、32.2%和5.2%(MV+HRS);增加水稻产量,以MV+HRS处理最高,4 a平均产量较RS、MV增加556.8和412.8 kg/hm2。2013和2015年,MV+HRS处理水稻产量高于MV+LRS。稻草-绿肥联合还田培肥地力效果明显,土壤有机质、全氮含量均比CK、RS和MV增加;且联合还田下有效养分提升更为全面。与稻草和绿肥单独应用相比,稻草-绿肥联合还田还能提升土壤微生物量氮及可溶性有机碳氮含量。可见,稻草-绿肥联合还田能够改善绿肥生长、提高水稻产量、提升土壤肥力;其中,高茬稻草与绿肥联合还田下的紫云英和水稻产量最高,土壤肥力也优于低茬处理,是综合利用稻草和绿肥资源的较好方式。  相似文献   

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