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1.
利用分子标记辅助育种技术选育高抗白叶枯病恢复系 总被引：15，自引：2，他引：13
通过常规回交育种结合分子标记辅助选择技术,已将来自IRBB24的2个抗白叶枯病基因, Xa21和Xa4, 聚合到感病的杂交稻恢复系绵恢７２５中。通过分子标记检测目标基因和亲本遗传背景差异分析快速获得了4个高抗白叶枯病的恢复系姊妹系R207 1、R207 2、R207 3和R207 4。采用与目标抗性基因相应的菲律宾菌系P1、P6 和7个中国病原型代表菌系（CⅠ~CⅦ）对4个姊妹系及其杂交组合进行田间接种。结果表明,这些姊妹系及所配制的杂交组合抗病性强、抗谱广,其中杂交组合G46A/R207 2具有良好的产量潜力,将R207 2定名为蜀恢207。 相似文献
2.
Yong-Li ZhouVeronica N.E. Uzokwe Cong-He ZhangLi-Rui Cheng Lei WangKai Chen Xiao-Qing GaoYong Sun Jin-Jie ChenLing-Hua Zhu Qi ZhangJauhar Ali Jian-Long Xu Zhi-Kang Li 《Crop Protection》2011,30(6):637-644
A novel bacterial blight (BB) resistance gene, Xa23, identified from Oryza rufipogon was introgressed into three popular restorer lines (Minghui63, YR293 and Y1671) for wild abortive cytoplasmic male sterility by marker-assisted backcross breeding approach in combination with artificial inoculation and stringent phenotypic selections. The three derived BB resistant restorer lines (Minghui63-Xa23, YR293-Xa23 and Y1671-Xa23) and their hybrid combinations with Zhenshan97A (Shanyou63-Xa23), NongfengA (Fengyou293-Xa23) and Zhong9A (Zhongyou1671-Xa23) demonstrated similar BB resistance spectrum as the donor parent, CBB23 (B). The newly developed BB resistant restorers and their derived hybrids were identical to their respective original versions for agronomic traits especially under disease free condition. However, under severe disease condition, the three BB resistant restorer lines exhibited significantly higher grain weight and spikelet fertility as compared to the respective original restorer lines thus further resulting in BB resistant hybrids with significantly higher grain yields than their respective popular original hybrids. The results indicated that the Xa23 gene could completely express its dominant broad spectrum resistance in different backgrounds of both restorer and male sterile lines across different growth stages, suggesting its immense breeding value in BB resistance improvement for hybrid rice. Moreover, a reasonable utilization and deployment of Xa23 gene for efficient control of BB disease in hybrid rice production was recommended. 相似文献
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香5是由湖北省农科院选育的优质两系杂交稻恢复系,所配组合广两优5号(广占63-4S/香5)于2013年通过了湖北省审定.利用回交和分子标记辅助选择技术,将供体亲本MD12086-1351中的抗稻瘟病基因Pi9、抗褐飞虱基因Bph14、Bph15和抗白叶枯病基因Xa23渗入到香5背景中,育成了3个同时携带Pi9、Bph14、Bph15和Xa23基因的新株系.鉴定结果表明,新株系的叶瘟抗性明显提高,穗颈瘟抗性部分提高,苗期抗褐飞虱,分蘖盛期高抗白叶枯病;产量、主要农艺性状、香味和稻米品质主要指标与香5相似.新株系所配的组合在产量、主要农艺性状上与香5所配的组合相似.表明新株系可以作为香5的替代系用于培育抗稻瘟病、抗褐飞虱和抗白叶枯病的两系杂交稻新组合. 相似文献
4.
ZHAN Xiao-deng ZHOU Hai-peng CHAI Rong-yao ZHUANG Jie-yun CHENG Shi-hua CAO Li-yong Chinese National Center for Rice Improvement State 《水稻科学》2012,19(1):29-35
Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight(BB) diseases,the two most prevalent diseases jeopardizing rice production.Rice hybrids with dural resistance to blast and BB are needed for sustainable production of food.An incomplete diallele design resulted in 25 crosses between five blast and five BB resistant germplasm accessions.Only one pair of parents,DH146 × TM487,showed polymorphism for all the markers to identify one blast resistance gene Pi25 and three BB resistance genes,Xa21,xa13 and xa5,thus it was used in the marker-assisted selection(MAS).F2 individuals of DH146 × TM487 were genotyped using flanking markers of RM3330 and sequence tagged site(STS) marker SA7 for Pi25.The resistant F2 plants with Pi25 were used for pyramiding BB resistance genes Xa21,xa13 and xa5 identified by the markers pTA248,RM264 and RM153,respectively in subsequent generations.Finally,after selection for agronomic traits and restoration ability among 12 pyramided lines,we acquired an elite restorer line,R8012 including all four target genes(Pi25+Xa21+xa13+xa5).Hybrid Zhong 9A/R8012 derived from the selected line showed stronger resistance to blast and BB,and higher grain yield than the commercial checks uniformally in experimental plots,2007 state-wide yield trial and 2008 nation-wide yield trial.This study provides a paradigmatic example to show that MAS is a practically feasible tool in effectively pyramiding multiple resistance genes.The resultant restoring line and its hybrid would play an important role in securing rice production in China. 相似文献
5.
分子标记辅助选择抗稻白叶枯病基因在选育水稻广亲和恢复系上的应用 总被引：18，自引：2，他引：18
以IRBB21为Xa21基因供体,广亲和恢复系4183为受体，进行1次杂交并回交3次，逐代用分子标记检测手段，导入广谱抗白叶枯病基因Xa21,在保持广亲和恢复系4183优良经济性状的基础上，增强其抗白叶枯病的能力，育成抗白叶枯病的广亲和恢复系抗4183，其抗性达到了IRBB21的抗性水平，且保持了4183的广亲和性、恢复性及优良的经济性状。并就杂交水稻白叶枯病的抗性改良及育种对策进行了讨论。 相似文献
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7.
四川主要水稻恢复系抗白叶枯病基因的分子检测 总被引：2，自引：0，他引：2
用分别与水稻抗白叶枯病基因Xa4、Xa7、Xa21和Xa23紧密连锁的PCR标记MP12、M3、pTA248和RM206对四川省新选育的以及主要应用的39份恢复系进行了分子检测，结果表明，这些恢复系均不含有Xa7（M3）和Xa21（pTA248）抗性等位点，17份携有榭（MP12）抗性等位点，少数（6个）携有Xa23（RM206）抗性等位点。在杂交水稻恢复系抗性改良中，应采用分子标记辅助选择（MAS）加快引入利用除工卅外的其它儿个显性抗性基因。 相似文献
8.
疣粒野生稻抗白叶枯病新基因的初步鉴定 总被引：1，自引：0，他引：1
以10个菲律宾白叶枯病菌小种和1个中国白叶枯病菌小种为供试菌系,以高感白叶枯病水稻品种IR24及携有不同抗白叶枯病基因的近等基因系IRBB1等16个材料作为参照,对粳稻品种8411/疣粒野生稻体细胞杂交获得的两个抗白叶枯病新种质SH5和SH76进行了白叶枯病抗谱鉴定。结果表明SH5和SH76在苗期的抗谱较广,并且与已知抗病基因的抗谱不同,但与IRBB5(xa5)和IRBB7(Xa7)相似。分别用xa5和Xa7的分子标记2F_1R和M5进行检测,确定SH5和SH76中不含有xa5和Xa7基因。初步推测SH5、SH76可能含有一个新的抗病基因或者一个连锁的基因簇群。 相似文献
9.
水稻三系恢复系镇恢084的螟虫抗性改良 总被引：1，自引：0，他引：1
以对螟虫有良好抗性的T1C - 19(含抗螟虫的cry1C*基因)作为供体,优良恢复系镇恢084为受体和轮回亲本,通过杂交、回交并结合分子辅助选择的方法将cry1C*基因导入镇恢084中,选育了携带cry1C*基因的纯合株系4个.对育成的抗螟虫新株系及其杂交组合的CRY1C*蛋白表达量、螟虫抗性、产量、主要农艺性状和稻米品质进行了研究.结果表明,4个抗虫新株系的CRY1C*蛋白表达量与供体T1C - 19基本一致,表明cry1C*基因在新株系中能够很好地表达;在自然条件下的田间调查结果显示,培育的4个抗螟虫株系及其所配杂交组合与供体亲本表现一致,都没有受到螟虫的危害,而受体亲本受到一定程度的螟虫危害.此外,新育成的4个抗螟虫株系在主要农艺性状、稻米品质和配合力上都与受体亲本镇恢084相近. 相似文献
10.
FU Guan-fu SONG Jian XIONG Jie LIAO Xi-yuan ZHANG Xiu-fu WANG Xi LE Ming-kai TAO Long-xing 《水稻科学》2012,19(4):309-314
Fifteen common rice maintainer lines and 26 high-yielding restorer lines were used to evaluate their thermal resistance and fertility during flowering and early grain filling stages.The rice plants were subjected to high temperature stress(39-43 ℃) for 1-15 d from main stem flowering.Based on the heat stress index,they were divided into thermal resistant lines,semi-thermal resistant lines,semi-thermal sensitive lines and thermal sensitive lines.Therefore,the maintainer lines K22B,Bobai B and V20B belonged to thermal resistant lines,whereas II-32B,Zhongzhe B and Zhong 9B belonged to thermal sensitive lines.For rice restorer lines,Minghui 63 had the highest thermal resistance,followed by R207,P32,P929,and the lowest thermal resistant lines P62-2-2,R8006 and P51.The correlation analysis indicated that the heat stress index was significantly correlated with seed-setting rate and abortive grain rate under heat stress,but not under natural conditions.This indicated that heat stress occurred during flowering and early grain filling stages mainly decreased the seedsetting rate and significantly increased the abortive grain rate in both rice maintainer and restorer lines. 相似文献
11.
Field resistances of nine accessions of common wild rice (Oryza rufipogon Griff.) and one rice variety (IR24) were evaluated by using nine strains of bacterial blight pathogen (Xanthomonas oryzae pv. oryzae) from the Philippines. IR24 was highly susceptible to all the strains, and six common wild rice accessions resisted all the nine strains, with a resistance frequency of 67%. The accessions Yulin and Wanning were only susceptible to PXO280 and PXO71, respectively. The accession Gaozhou was susceptible to the three strains PXO79, PXO99 and PXO339, whereas resistant to the other six strains. It could be concluded that there is at least one resistance gene in each common wild rice accession. The functional markers of the genes xa5, xa13, Xa21 and Xa27 were used to detect the presence of these resistance genes in the nine tested wild rice accessions, and it was found that four wild rice accessions contained heterozygous xa13. Among the nine common wild rice accessions, five were homozygous for Xa27 and three homozygous for xa27, and the accession Laibin contained neither xa27 nor Xa27. In addition, there were no xa5 and Xa21 in all of these accessions. 相似文献
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滇型杂交粳稻亲本种质资源抗瘟性研究 总被引：5，自引：0，他引：5
１９９３～１９９６年，测定了８０６份滇型杂交粳稻种质资源的抗瘟谱，筛选出抗谱较广的材料６４份。探讨了恢复系对杂种Ｆ１抗瘟性的贡献，提出了利用恢复系进行杂交粳稻抗病基因布局来控制稻瘟病发生的育种对策。 相似文献
14.
利用功能标记鉴定普通野生稻中的白叶枯病抗性基因 总被引：1，自引：0，他引：1
以9个菲律宾白叶枯病菌小种对供试的9份普通野生稻（Oryza rufipogon Griff.）及1份高感白叶枯病材料IR24进行抗性鉴定,发现IR24对所有的菌株都表现为高感, 6份野生稻材料对9个菌株表现全抗,占参试野生稻总数的67%。取自广西玉林的1份材料只感PXO280(P8),海南万宁的1份材料感PXO71(P4),广州高州的1份材料对PXO79、PXO99和PXO339感病,而这几份材料对其余菌株都表现为抗病,说明每份材料至少含有1个抗性基因。利用已克隆的白叶枯病抗性基因xa5、xa13、Xa21和Xa27的功能标记检测,结果表明9份供试普通野生稻中都不含抗性基因xa5、Xa21;5份为显性Xa13纯合体,4份为隐性抗病xa13杂合体;5份为抗病显性Xa27纯合体,3份为隐性xa27纯合体,1份材料中xa27和Xa27都不存在。 相似文献
15.
《Journal of Crop Improvement》2013,27(1):101-116
Abstract In pathogen population analysis of 208 Xanthomonas oryzae pv. oryzae(Xoo) strains that were assembled from different parts of India, 21 pathotypes were identified on the basis of disease reactions on near-isogenic lines (NILs) and 13 pathotypes, on rice differentials. Rice cultivars, Jyothi and IR50, which are high yielding but highly prone to bacterial blight (BB) caused by pathogen populations of Xanthomonas oryzae pv. oryzae in India, were chosen. To improve the BB resistance of these two varieties, a pyramid line, NH56, containing four R-genes, Xa4, xa5, xa13, and Xa21, was selected as the R-donor based on resistance to existing pathogen population. The four R-genes were successfully transferred to cultivars through a traditional backcross method and their presence was documented with marker-aided selection (MAS). Thirty BC4F2 plants derived from JxNH56 (cv. Jyothi) and 45 BC4F2 plants derived from IR50xNH56 (cv. IR50) had all four resistance genes (Xa4, xa5, xa13, and Xa21), which should be useful resistance donors for breeding other BB-resistant elite indica varieties. 相似文献
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中国常用水稻保持系及恢复系开花灌浆期耐热性评价 总被引：2，自引：0，他引：2
以我国常用的15个水稻保持系和26个丰产性较好的恢复系为材料,于主茎见穗当天起连续15 d进行高温胁迫处理（9:00-15:00,39~43 ℃）,研究花期高温胁迫对水稻保持系和恢复系结实特性的影响。结果表明,根据高温胁迫指数,可把水稻品种分为热钝感型、耐热型、不耐热型和热敏感型等4个类型。其中,水稻保持系K22B、博白B和V20B为热钝感型材料,Ⅱ 32B、中浙B和中9B为热敏感型材料;水稻恢复系明恢63耐热性最强,其次为R207和P929,P62 2 2、R8006 和P51等耐热性最弱。进一步分析表明,高温胁迫指数与水稻在自然条件下结实率、空壳率和秕谷率之间相关不显著,但与胁迫环境下结实率和空壳率的相关性均达极显著水平。花期高温胁迫致使水稻保持系和恢复系结实率下降,空壳率大幅度上升。 相似文献
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滇型杂交粳稻主要亲本的SSR 指纹图谱及其遗传差异分析 总被引：1，自引：0，他引：1
用筛选的13对SSR引物,构建了41个水稻品种(包括35份滇型杂交粳稻恢复系、保持系材料、2个杂交种以及4个籼稻品系)的分子指纹图谱.聚类分析表明,41个水稻品种间遗传相似系数在0.52～0.94之间;在相似系数0.52处,可以将粳稻与籼稻区分开;在相似系数0.66处,可将杂交粳稻与其它粳稻品系区分开;在相似系数0.71处,又可将大部分恢复系与保持系区分开.生产中应用的一些强优势滇型杂交粳稻的亲本分别聚于不同的类群,表明亲本间的遗传差异较大. 相似文献
18.
聚合白背飞虱和褐飞虱抗性基因创制杂交水稻恢复系 总被引：1，自引：1，他引：1
【目的】为了创制兼抗白背飞虱和褐飞虱的水稻恢复系，【方法】分别以抗褐飞虱材料B5（携带褐飞虱抗性基因Bph14和Bph15）及携带白背飞虱抗性位点qsI-4的籼型恢复系福恢7011为供体亲本，以骨干恢复系福恢676为轮回亲本，应用低世代分离群体田间表型结合单株鉴定与高世代稳定株系室内筛选和分子标记辅助选择相结合的方法，并对抗虫株系及其测交后代进行考查和农艺性状分析。【结果】选育出聚合Bph14、Bph15 和qsI-4的恢复系材料3份，携带2个抗虫基因的恢复系材料3份。其中6份恢复系的褐飞虱抗性鉴定结果均表现中抗以上。通过抗性鉴定和杂交后代农艺性状分析筛选出具有生产应用潜力的恢复系材料2份。【结论】为褐飞虱和白背飞虱抗性聚合新种质的创制和应用提供了基础材料。 相似文献
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【目的】为了创制兼抗白背飞虱和褐飞虱的水稻恢复系,【方法】分别以抗褐飞虱材料B5(携带褐飞虱抗性基因Bph14和Bph15)及携带白背飞虱抗性位点qsI-4的籼型恢复系福恢7011为供体亲本,以骨干恢复系福恢676为轮回亲本,应用低世代分离群体田间表型结合单株鉴定与高世代稳定株系室内筛选和分子标记辅助选择相结合的方法,并对抗虫株系及其测交后代进行考查和农艺性状分析。【结果】选育出聚合Bph14、Bph15和qsI-4的恢复系材料3份,携带2个抗虫基因的恢复系材料3份。其中6份恢复系的褐飞虱抗性鉴定结果均表现中抗以上。通过抗性鉴定和杂交后代农艺性状分析筛选出具有生产应用潜力的恢复系材料2份。【结论】为褐飞虱和白背飞虱抗性聚合新种质的创制和应用提供了基础材料。 相似文献
20.
杂交粳稻同核异质不育系细胞质效应的研究 总被引：14，自引：1，他引：13
以具有ChinsurahBoroⅡ（BT）、滇型（D）、野败型（WA）和野栽型（WC）4种不育细胞质的不育系南粳11A和南粳34A及其相应保持系与77302－1、宁恢3－2、C57和C堡4个恢复系配制的杂种F1为材料,探讨了不育系细胞质对F1代12个性状的遗传效应。不育系细胞质对F1代的株高、穗颈长、每稳实粒数、结实率、收获指数和单株产量等性状有明显的负效应,而对稳数、每穗总粒数、千粒重和生物产量则有明显的正效应。不育系细胞质的这种遗传效应随细胞质来源的不同表现明显的差异,D型和BT型细胞质在多数性状上的表现优于WA型和WC型细胞质。此外,不育系细胞质与保持系细胞核和恢复系细胞核及三者之间的互作在多数性状上也有明显差异。 相似文献