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1.
Hasna Hena Begum Mitsuru Osaki Takuro Shinano Hiroaki Miyatake Jun Wasaki Takuya Yamamura Toshihiro Watanabe 《Soil Science and Plant Nutrition》2005,51(4):497-506
Transgenic rice ( Oryza sativa L., a C3 plant) lines carrying a complete phospho enol pyruvate carboxylase (PEPC) gene from maize (a C4 plant) were tested for their performance in terms of organic acid synthesis and organic acid exudation into the rhizosphere under phosphorus (P)-deficient conditions. High PEPC activity increased the fraction of photosynthetically fixed carbon allocated to the organic acid pool, and P deficiency enhanced oxalate exudation from the roots of the transgenic plants. There was no evidence that the transformed PEPC was involved in internal P recycling in the plant. However, the root PEPC activity was positively correlated with the oxalate exudation and negatively correlated with the root P concentration, and a higher root PEPC activity led to a higher oxalate exudation. Thus, it is suggested that C4 -PEPC transgenic rice plants had acquired the ability to exude oxalate, which enhanced their capacity to adapt to low P soil conditions. 相似文献
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Organic acid anions exuded from roots of Picea abies (Norway spruce) seedlings grown on glass beads in the presence and absence of mycorrhiza (Laccaria bicolor) and aluminum (Al) at pH 3.9 were measured. We wanted to test if the roots exuded more organic acid anions when exposed to Al and if mycorrhization influenced the exudation. Oxalate was exuded in far higher amounts than any other organic acid anion, with a maximum rate of 1.7 nmol (mg root DW)–1 d–1. Mycorrhizal roots exuded significantly more oxalate than nonmycorrhizal roots. The presence of Al did not enhance oxalate exudation. We conclude that the oxalate exuded constitutively by Picea abies/Laccaria bicolor may lead to rhizosphere oxalate concentrations that are relevant for Al resistance. 相似文献
4.
The effects of low aluminum (Al) activity in nutrient solution on the concentrations of organic acids in two cultivars of maize (Zea mays L.), HS7777 Al‐sensitive and C525‐M Al‐tolerant, were studied. Aluminum stress increased total organic acid concentration in the roots and in the shoots for both cultivars. The relative increase of t‐aconitic, citric, formic, malic, and quinic acids was higher in the roots than in the shoots for both cultivars. The concentrations of c‐aconitic, isocitric, malonic, oxalic, and succinic organic acids were reduced by Al stress, principally for C525‐M. There were no consistent differences in organic acid concentrations between the cultivars to discriminate Al tolerance. The Al tolerance for C525‐M may be justified by lower Al concentrations in the root tips where cellular division takes place and/or by higher excretion of organic acids from roots to the rhizosphere for detoxification of Al by chelation. 相似文献
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Phytotoxicity of aluminum (Al) has become a serious problem in inhibiting plant growth on acid soils. Under Al stress, the changes of rhizosphere pH, root elongation, absorption of Al by wheat roots, organic acids exuded from roots, and some main factors related to Al-tolerant mechanisms have been studied using hydroponics, fluorescence spectrophotometry, and high performance liquid chromatography (HPLC). Two wheat cultivars, Brevor and Atlas66, differing in Al tolerance are chosen in the study. Accordingly, the rhizosphere pH has a positive effect on Al tolerance. Atlas66 (Al-tolerant) has higher capability to maintain high rhizosphere pH than Brevor (Al-sensitive) does. High pH can reduce Al3+ activity and toxicity, and increase the efficiency of exuding organic acids from the roots. More inhibition of root elongation has been found in Brevor because of the exposure of roots to Al3+ solution at low pH. Brevor accumulate more Al in roots than Atlas66 even at higher pH. Al-induced exudation of malic and citric acids has been found in Atlas66 roots, while no Al-induced organic acids have been found in Brevor. These results indicate that the Al-induced secretion of organic acids from Atlas66 roots has a positive correlation with Al tolerance. Comprehensive treatment of Al3+ and H+ indicates that wheat is adversely influenced by excess Al3+, rather than low pH. 相似文献
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Koranda M Schnecker J Kaiser C Fuchslueger L Kitzler B Stange CF Sessitsch A Zechmeister-Boltenstern S Richter A 《Soil biology & biochemistry》2011,43(3):551-558
Plant roots strongly influence C and N availability in the rhizosphere via rhizodeposition and uptake of nutrients. This study aimed at investigating the effect of resource availability on microbial processes and community structure in the rhizosphere. We analyzed C and N availability, as well as microbial processes and microbial community composition in rhizosphere soil of European beech and compared it to the bulk soil. Additionally, we performed a girdling experiment in order to disrupt root exudation into the soil. By this novel approach we were able to demonstrate that enhanced resource availability positively affected N mineralization and hydrolytic enzyme activities in the rhizosphere, but negatively affected nitrification rates and oxidative enzyme activities, which are involved in the degradation of soil organic matter. Both rhizosphere effects on N mineralization and oxidative enzyme activities disappeared in the girdling treatment. Microbial community structure in the rhizosphere, assessed by phospholipid fatty acid analysis, differed only slightly from bulk soil but was markedly altered by the girdling treatment, indicating additional effects of the girdling treatment beyond the reduction of root exudation. Differences in oxidative enzyme activities and nitrification rates between rhizosphere soil and bulk soil, however, suggest considerable differences in the (functional) microbial community composition. 相似文献
7.
铝和镉胁迫对两个大麦品种矿质营养和根系分泌物的影响 总被引：7，自引：0，他引：7
A hydroponic experiment was carried out to study the effect of aluminum （Al） and cadmium （Cd） on Al and mineral nutrient contents in plants and Al-induced organic acid exudation in two barley varieties with different Al tolerance. Al- sensitive cv. Shang 70-119 had significantly higher Al content and accumulation in plants than Al-tolerant cv. Gebeina, especially in roots, when subjected to low pH （4.0） and Al treatments （100 μmol L^-1 Al and 100 μmol L^-1 Al ＋1.0 μmol L^-1 Cd）. Cd addition increased Al content in plants exposed to Al stress. Both low pH and Al treatments caused marked reduction in Ca and Mg contents in all plant parts, P and K contents in the shoots and leaves, Fe, Zn and Mo contents in the leaves, Zn and B contents in the shoots, and Mn contents both in the roots and leaves. Moreover, changes in nutrient concentrations were greater in the plants exposed to both Al and Cd than in those exposed only to Al treatment. A dramatic enhancement of malate, citrate, and succinate was found in the plants exposed to 100 μmol L^-1 Al relative to the control, and the Al-tolerant cultivar had a considerable higher exudation of these organic acids than the Al-sensitive one, indicating that Al-induced enhancement of these organic acids is very likely to be associated with Al tolerance. 相似文献
8.
Cultivated tea (Camellia sinensis) plants acidify the rhizosphere, and Aluminum (Al) toxicity is recognized as a major limiting factor for plant growth in acidic soils. However, the mechanisms responsible for rhizosphere acidification associated with Al have not been fully elucidated. The present study examined the effect of Al on root-induced rhizosphere acidification, plasma membrane H+-adenosine triphosphatase (H+-ATPase) activity, and cation-anion balance in tea plant roots. The exudation of H+ from tea plant roots with or without Al treatment was visualized using an agar sheet with bromocresol purple. The H+-ATPase activity of plasma membranes isolated from the roots was measured after hydrolysis using the two-phase partition system. The Al treatment strongly enhanced the exudation of H+, and the acidification of tea plant roots by Al was closely associated with plasma membrane H+-ATPase activity. The root plasma membrane H+-ATPase activity increased with Al concentration. The Al content, amount of protons released, and H+-ATPase activity were significantly higher in roots treated with Al than in those untreated. The results of the cation-anion balance in roots showed an excess of cations relative to anions, with the amount of excess cation uptake increasing with increasing Al concentrations. These suggest that Al-enhanced proton release is associated with plasma membrane H+-ATPase activity and excess cation uptake. Findings of this study would provide insights into the contributing factors of soil acidification in tea plantations. 相似文献
9.
Hui Ming Luo Toshihiro Watanabe Takuro Shinano Toshiaki Tadano 《Soil Science and Plant Nutrition》2013,59(4):897-907
Aluminum (Al) tolerance and phosphate absorption in rape and tomato were compared under water culture and field conditions. The relative growth rate in the Al treatment compared with -A1 treatment was similar in the two crops under water culture conditions, while under field conditions, the growth rate was 2- to 3-fold higher in rape than in tomato in spite of the higher Al concentration in the soil solution than in the culture solution. The relative amount of phosphate absorbed in the Al treatment compared with - Al was not appreciably different between rape and tomato under water culture conditions, while under field conditions, it was 3- to 6-fold larger in rape than in tomato. The exudation rate of citric acid by roots was much higher in rape than in tomato. The plant growth, root elongation, and amount of phosphate absorbed in rape were inhibited in the 150 µM Al in the culture solution. However, the inhibition was alleviated by the addition of 200 µM citric acid or 500 µM malic acid. The P concentration in the culture solution decreased by the presence of Al as aluminum phosphate. However, addition of citric and malic acids increased the amount of phosphate released from the precipitated aluminum phosphate. In conclusion, one of the mechanisms for the higher Al tolerance and larger phosphate absorption in rape than in tomato under field conditions was ascribed to the higher concentration of exuded citric acid by Al in the rhizosphere. It was suggested that the exudation of citric acid might contribute to the detoxification of Al and to the increase phosphate availability in the rhizosphere in rape. 相似文献
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Purpose
Genetic modification of Bt rice may affect straw decomposition and soil carbon pool under flood conditions. This study aims to assess the effects of cry gene transformation in rice on the residue decomposition and fate of C from residues under flooded conditions.Materials and methods
A decomposition experiment was set up using 13C-enriched rice straws from transgenic and nontransgenic Bt rice to evaluate the soil C dynamics and CH4 or CO2 emission rates in the root and non-root zones. The concentrations and stable carbon isotope compositions of the soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC), CH4, and CO2 of the root and non-root zones were determined from 7 to 110 days after rice straw incorporation.Results and discussion
Rice straw incorporation into soil significantly increased the SOC, DOC, and MBC concentrations and the CH4 and CO2 emission rates. The percentage of 13C-SOC remaining in the root zone was significantly lower than that in the non-root zone with rice straw decomposition. The DOC and MBC concentrations significantly increased in both the root and non-root zones between 0 and 80 days after rice straw incorporation. However, no significant differences were found after Bts (Bt rice straw added into soil) and Cks (nontransgenic Bt rice straw added into soil) incorporation in the root and non-root zones. This result may be attributed to the priming effects of sufficient oxygen and nutrients on straw degradation in the root zone.Conclusions
Bt gene insertion did not affect the SOC, DOC, and MBC concentrations and the CH4 and CO2 emission rates in both the root and non-root zones. However, rice straw incorporation and root exudation significantly increased the SOC, DOC, and MBC concentrations and the CH4 and CO2 emission rates. 相似文献12.
Exudation of organic anions by roots of cabbage,carrot, and potato as influenced by environmental factors and plant age 总被引：1，自引：0，他引：1
A previous study demonstrated that cabbage was P efficient compared to carrot and potato. However, calculating plant P uptake by a mechanistic simulation model based on P transport by diffusion and mass flow, P uptake of roots according to the Michaelis‐Menten kinetics, and morphological root characteristics including root hairs, revealed that these parameters could explain only 2/5 of the total P uptake of cabbage, but 4/5 of that of carrot and potato (Dechassa et al., 2003). Therefore, it was hypothesized that a higher root exudation of organic anions may enhance P mobilization and hence P uptake of cabbage. The objective of this research was to determine root exudation of organic anions by the three species, and to investigate the influence of plant age and dark/light period on organic‐anion exudation by cabbage. Experiments were conducted in a growth chamber in nutrient solution with or without P. Organic anions were determined in root exudates and in root tissue. With cabbage and potato, P deficiency induced exudation of citrate and succinate, respectively. Citrate‐exudation rate of P‐deficient cabbage plants was correlated with accumulation of citrate in root tissue. In contrast, high succinate‐exudation rates in potato were not correlated with an increased concentration in root tissue. For carrot, no change was observed in the exudation of any of the organic anions in response to P deficiency. The results also showed that succinate‐ and citrate‐exudation rates of cabbage roots increased with increased plant age. There was also a significant increase in exudation rates of organic anions of cabbage roots during the light period of the day. It was concluded that cabbage had the ability to exude large amounts of citrate in response to P deficiency by which it can additionally enhance its P‐uptake efficiency, whereas carrot and potato showed little evidence of possessing such a mechanism. 相似文献
13.
Rapid nitrogen（N） transformations and losses occur in the rice rhizosphere through root uptake and microbial activities. However,the relationships between rice roots and rhizosphere microbes for N utilization are still unclear. We analyzed different N forms（NH＋4,NO-3, and dissolved organic N）, microbial biomass N and C, dissolved organic C, CH4 and N2O emissions, and abundance of microbial functional genes in both rhizosphere and bulk soils after 37-d rice growth in a greenhouse pot experiment. Results showed that the dissolved organic C was significantly higher in the rhizosphere soil than in the non-rhizosphere bulk soil, but microbial biomass C showed no significant difference. The concentrations of NH＋4, dissolved organic N, and microbial biomass N in the rhizosphere soil were significantly lower than those of the bulk soil, whereas NO-3in the rhizosphere soil was comparable to that in the bulk soil. The CH4 and N2O fluxes from the rhizosphere soil were much higher than those from the bulk soil. Real-time polymerase chain reaction analysis showed that the abundance of seven selected genes, bacterial and archaeal 16 S rRNA genes, amoA genes of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, nosZ gene, mcrA gene, and pmoA gene, was lower in the rhizosphere soil than in the bulk soil, which is contrary to the results of previous studies. The lower concentration of N in the rhizosphere soil indicated that the competition for N in the rhizosphere soil was very strong, thus having a negative effect on the numbers of microbes. We concluded that when N was limiting, the growth of rhizosphere microorganisms depended on their competitive abilities with rice roots for N. 相似文献
14.
为探讨铝(Al)胁迫条件下脱落酸(ABA)调控植物根系有机酸分泌的机制,进行了ABA与Al诱导大豆根系柠檬酸分泌的关系试验。结果表明:1)外源ABA和ABA合成抑制剂fluridone分别提高和降低了Al诱导的大豆根尖ABA含量的增加,但对根系柠檬酸分泌量均无影响,ABA对根系内源柠檬酸含量和柠檬酸合成酶的活性也没有影响;2)分根试验表明,与Al直接接触的根部(Part A)内源ABA含量发生变化,且有柠檬酸的分泌,而不与Al直接接触的根部(Part B)内源ABA含量也发生变化,但没有柠檬酸分泌;3)Al胁迫下,大豆耐Al基因型柠檬酸分泌量远高于敏感基因型,但二者的内源ABA含量却没有差异;4)30μmol AlCl3处理,在0~12 h柠檬酸分泌速率和内源ABA含量随Al处理时间增加而增加,去除Al胁迫时(12~18 h),柠檬酸分泌速率继续增加,但内源ABA含量则迅速下降。综合以上结果,推测ABA不是通过提高Al诱导柠檬酸分泌来调控大豆耐Al性。 相似文献
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Mesfin Tesfaye Nicholas S DufaultMelinda R Dornbusch Deborah L AllanCarroll P Vance Deborah A Samac 《Soil biology & biochemistry》2003,35(8):1103-1113
Transgenic alfalfa over-expressing a nodule-enhanced malate dehydrogenase (neMDH) cDNA and untransformed alfalfa plants were grown at the same field site and rhizosphere soils collected after 53 weeks of plant growth. These alfalfa lines differ in the amount and composition of root organic acids produced and exuded into the rhizosphere. Nucleotide sequencing of PCR-based 16S ribosomal DNA (rDNA) clone libraries and Biolog™ GN microtiter plates were employed to assess the activity of naturally occurring rhizobacteria in the two alfalfa rhizospheres. Selected macro- and micro-elements in the two alfalfa rhizosphere soils were also measured. Analysis of 240 16S rDNA clone sequences indicated the existence of about 11 bacterial phyla and their major subdivisions in the two alfalfa rhizosphere samples. There were qualitative changes in the abundance of bacterial phylogenetic groups between rhizosphere soils of transgenic and untransformed alfalfa. Carbon substrate utilization profiles suggested that rhizosphere samples from transgenic alfalfa had significantly greater microbial functional diversity compared with rhizosphere samples from untransformed alfalfa. The concentrations of nitric acid extractable P, K, Mn, Zn and Cu increased significantly in the transgenic alfalfa rhizosphere compared with the untransformed alfalfa rhizosphere. These observations indicate that organic acids produced by plant roots significantly influence rhizosphere microbial diversity and availability of macro- and micro-nutrients and demonstrate the utility of such trangenic plants as tools for studying the potential impact of plant root exudates on soil microbial ecosystems. 相似文献
16.
复合污染土壤中水稻根际元素特性及效应研究 总被引：1，自引：1，他引：0
【目的】以广东大宝山重金属复合污染农田为生长介质,通过研究水稻不同部位生长量、 金属含量、 对金属的富集系数,及其与根际、 非根际土金属含量、 形态变化的相关关系,探讨根际效应可能对水稻体内金属积累转运以及生物量的影响。【方法】选取了广东大宝山稻田重金属复合污染(As、 Pb、 Fe、 Cu、 Zn)土壤及当地常见的20个水稻品种进行根际袋试验,即将根际袋内的土视为根际土,根际袋外的土视为非根际土,将供试水稻品种种植于根际袋土壤中60天后收获,测定水稻各部位的生长量、 不同金属的含量,根际土和非根际土中各金属有效态的含量。【结果】Fe、 Cu、 Pb、 Zn、 As在根部的富集系数均大于其在茎叶的富集系数,各金属在茎叶和根部的富集能力排序分别为Zn Cu As ≈ Pb ≈ Fe和Fe Zn As Cu Pb。根际土和非根际土中各种金属有效态含量均为Fe Cu Pb Zn As。研究还发现,有效态Fe、 Cu和Zn浓度对整株干重的影响显著,作用强弱顺序为Cu Zn Fe,对水稻生长影响作用显著的三种有效态金属Fe、 Cu和Zn均为植物生长所必需的元素。供试土壤中有效态Cu浓度对水稻的生长所起的作用最强。根际土有效态Fe浓度对根系Fe的积累作用效果显著,有效态As浓度显著抑制了根系Fe的积累,且有效态As浓度的作用强于有效态Fe。【结论】根际土中有效态Fe对株高、 根干重、 茎叶干重和整株干重均起着抑制作用,有效态Cu对水稻生长起到了促进作用。根际土有效态As和非根际土有效态Zn对根部Fe的积累起到了抑制作用,根际土有效态Fe和非根际土有效态Cu则起到了促进作用。非根际土有效态Fe和有效态Zn对水稻根长的增加均起到了促进作用。 相似文献
17.
Rice roots and CH4 oxidation: the activity of bacteria, their distribution and the microenvironment 总被引：9，自引：0，他引：9
Irrigated rice fields account for 10–30% of global methane emissions. Rice plants ventilate the soil and enlarge the oxic–anoxic interface by their root system, thus supplying the necessary O2 to aerobic CH4 oxidizing bacteria (MOB). Rice plants (Oryza sativa type japonica var. Roma) were grown in microcosms in a greenhouse. The roots were sandwiched between two blocks of flooded rice field soil separated by a nylon gauze bag. A root mat developed which mimicked the dense root texture in the upper layer of a natural rice field. Flux measurements under oxic and anoxic conditions showed that CH4 was oxidized with a constant rate of 19% of the anoxically emitted CH4, suggesting that CH4 oxidation in the rhizosphere was at least sometimes limited by CH4 availability. Washed rice roots could both produce and oxidize CH4, depending upon incubation conditions. CH4 production by washed rice roots accounted for at most 10% of the CH4 emitted under anoxic conditions. Initial CH4 oxidation rates of washed roots equaled oxidation rates calculated from the difference between oxic and anoxic fluxes in situ. Oxidation rates became twice as high after an induction period of 20 h, indicating a limitation by O2 or CH4 in situ. The micro-environmental conditions near to the root mat were measured using microelectrodes for O2, redox potential and NH4+ and diffusion probes for CH4. Up to 42 μM O2 was detected in the root mat and concentrations were >2.5 μM in 45% of all measurements. In the bulk soil, no O2 was detected below 2 mm depth, but the root mat significantly increased the redox potential. Plant roots and associated bacteria decreased porewater CH4 and NH4+ concentrations. In the root mat, concentrations of dissolved CH4 were below the detection limit of our probes (<5 μM). Cell numbers of MOB increased with time in the rhizosphere and in the rhizoplane. MOB and aerobic heterotrophic bacteria (AHB) each numbered from 106 to 108 cells g−1 dry weight of soil or root biomass). Active MOB occurred near to a root mat similar to the dense root texture in the upper layer of rice fields. We speculate about O2 or CH4 limitation of MOB. 相似文献
18.
Gibberellin A3 or 2,4-dichlorophenoxyacetic acid were applied to the foliage of peanut plants under axenic culture and in the greenhouse. Leaves, roots and exudates from axenically grown plants were analyzed for total lipids, free sterols, free fatty acids and paraffinic hydrocarbons. The total lipid concentrations of leaves, roots and root exudates were not altered. The free sterol of roots, and the paraffinie hydrocarbon concentrations of both leaves and roots increased, but the free fatty acid of root exudates decreased. Plants treated in the greenhouse were rated for disease severity after soil infestation with Pythium myriotylum. Roots of treated plants exhibited less rot than roots of non-treated plants. We believe there are possibilites of altering disease susceptibility using foliar applications of growth regulators through their effects on root lipids and root-lipid exudation patterns. 相似文献
19.
The Effects of AlCl3 Additions on Rhizosphere Soil and Fine Root Chemistry of Sugar Maple (Acer Saccharum) 总被引：1，自引：0，他引：1
Increased Al mobilization and Ca and Mg leaching have been linked to nutritional imbalances in sugar maple across the northeastern US and Canada. The susceptibility of sugar maple fine roots to Al stress is poorly understood, in part because roots respond to Al stress by altering the chemistry of the rhizosphere. AlCl3 was applied to plots of sugar maple at the Hubbard Brook Experimental Forest, NH. After two years of treatment, we sampled fine roots of sugar maple, rhizosphere soil, and bulk soil in the Oa horizon and the upper 10 cm of the mineral soil. AlCl3 treatments resulted in significantly less Ca (21%) and Mg (30%) in fine roots from the organic horizon, but had no significant effect on fine root Al. Fine root (Ca+Mg):Al ratios were 42% lower in AlCl3 plots than in controls, though most roots had ratios above critical toxicity thresholds developed for hydroponically grown sugar maple seedlings. In the mineral horizon, roots differed only in Mg concentration, which was 22% lower in AlCl3 plots. In the AlCl3 treated plots, rhizosphere soil in the organic horizon had 47% greater Al and 29% less Mg than in controls. Combining data from both treatments we found significantly less Al and organically bound Al in rhizosphere soil than in bulk soil, possibly due to leaching of Al from the rhizosphere by organic acids released by roots. These results suggest that increased mobilization of Al in soil lowers (Ca+Mg):Al ratios in sugar maple fine roots, though roots may minimize Al stress by leaching Al from the rhizosphere. 相似文献
20.
The wheat (Triticum aestivum L.) cultivar Yangzhou 158 was used as a reference. The wheat root exudates were collected using a hydroponic mode. The changes of the electrolytes, H+, sugar, organic acids, amino acids, and secondary metabolites in wheat root exudates induced by aluminum (Al) were studied. The research results show that Al stress affects wheat root exudation. The secreted electrolytes and sugar increase with the increasing of the external Al3+ concentration. The total amount of secreted amino acids has a specific correlation with the external Al3+ concentration. At first, the amino acids secrete normally, but when Al3+ concentration is over 10 mg.L-1, the amino acid constitution varies obviously. Under Al stress, some original secondary metabolites disappear gradually, and other new secondary metabolites release simultaneously. Increasing the external Al3+ concentration gradually stimulates the exudation of organic acids. The organic acid levels in the wheat root zone increase in response to Al treatments. Active Al ions are accumulated in wheat roots. This Al-dependent variation in wheat root exudates suggests a specific Al-induced response of the wheat. 相似文献