共查询到20条相似文献，搜索用时 421 毫秒
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120只SPF级昆明种雌性小鼠平均分成3组，菌草灵芝孢子粉分三个剂量组给小鼠灌胃，共30d。测定小鼠的体重、免疫器官脏器／体重比值、迟发型变态反应（DTH）、ConA诱导的小鼠淋巴细胞转化能力、抗体生成细胞数、半数溶血值（HC50）、单核一巨噬细胞碳廓清、巨噬细胞吞噬鸡红细胞吞噬率指标。结果：0．150g／kg．bw剂量能提高小鼠的半数溶血值（P〈0．05）；0．450g／kg．bw剂量能提高小鼠迟发型变态反应（DTH）能力、ConA诱导的淋巴细胞转化能力、增加抗体生成细胞数（P〈0．05）；菌草灵芝孢子粉对小鼠体重增长、胸噱体重比值、脾脏体重比值、单核-巨噬细胞碳廓清能力、小鼠巨噬细胞吞噬鸡红细胞的能力均无明显影响（P〉0．05）。 相似文献
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《中国食用菌》2016,(6)
探讨灵芝(Ganoderma lucidum)孢子油提取物复合制剂对小鼠急性酒精肝损伤的保护作用。分别设定0.167 g·kg~(-1)、0.333 g·kg~(-1)、1.00 g·kg~(-1)三个剂量组,以及乙醇模型对照组和空白对照组。各剂量组经口灌胃给予受试物,溶剂对照组和乙醇模型组给予食用植物油。30 d后,用乙醇建立急性酒精性肝损伤小鼠模型。16 h后处死动物,测定肝组织中甘油三酯(TG)、谷胱甘肽(GSH)、丙二醛(MDA),同时取肝脏进行组织病理学检查。结果表明样品可以降低3个剂量组小鼠急性酒精性肝损伤后第16小时肝组织中TG含量和中剂量组MDA含量,肝脏组织病理学检查发现,乙醇模型对照组动物肝损伤程度高于溶剂对照组(P0.05)。结论证明灵芝孢子油提取物复合制剂对急性酒精肝损伤具有辅助保护功能。 相似文献
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灵芝多糖降血糖作用的研究 总被引：6，自引：0，他引：6
目的：研究灵芝多糖对四氧嘧啶致高血糖小鼠、去甲肾上腺素致高血糖小鼠及正常小鼠血糖水平的影响。方法：制备四氧嘧啶致糖尿病小鼠模型及去甲肾上腺素致高血糖小鼠模型，给药2周后取血测定血糖水平。结果：灵芝多糖能明显降低四氧嘧啶所致糖尿病小鼠及去甲肾上腺索所致高血糖小鼠的血糖水平，其中高剂量组与模型对照组相比均有显著意义（P〈0．05），而对正常小鼠血糖水平影响较小，低、中、高剂量组与正常对照组相比均无显著意义（P〉0．05）。结论：灵芝多糖对四氧嘧啶致高血糖小鼠及去甲肾上腺素致高血糖小鼠具有明显降血糖作用，而对正常小鼠血糖水平影响较小。 相似文献
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１．本文较详细地把笔者多年来对原木灵芝精粉成份所进行的检测数据整理成文，其中包括原木灵芝精粉主要化学成分、三大营养素、微量元素、氨基酸组成、重金属及有害物含量、微生物含量、其他等，这些数据仅供参考。２．浅述原木灵芝精粉各成份对治疗疾病的药理作用。３．分析高纯度的“原木灵芝精粉”的三大特点，即原料优良、加工设备先进完善、工艺技术条件先进合理。 相似文献
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对以糙米浆、麦芽汁和黄豆芽汁为培养基组成的灵芝深层发酵条件进行优化，结果表明：灵芝深层发酵的起始pH5．5、接种量10％、装液量40mL和接种菌龄5d时可以获得最大灵芝菌丝干重。方差分析表明装液量和接种菌龄对灵芝生长有显著影响。在优化发酵工艺条件下，发酵第7d胞外多糖产量达到5．7g／L。 相似文献
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LIU Guang-jie LIU Guang-ze LAN Dan CHEN Mei-juan LIAO Han-xiong LIU Hai-xia ZOU Qing-yan 《园艺学报》2013,29(6):1114-1118
AIM: To evaluate the effect of betaine on lipid metabolism disorder in inherited db/db mice with long-term nonalcoholic fatty liver disease (NAFLD).METHODS: Experimental NAFLD models were established by feeding the db/db mice with high-fat diet. Fifty 7-month-old db/db mice were randomly divided into 5 groups: the mice in low, medium and high dose groups were given betaine by intragastric administration at doses of 200 mg/kg, 400 mg/kg and 800 mg/kg for 6 weeks, respectively,while the mice in saline control group and positive drug group were given normal saline and positive control drug,respctively. All the animals were killed, and serum alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and glucose tolerance were detected. The pathological changes of the liver tissues were also observed.RESULTS: Betaine significantly decreased the levels of ALT, TC and LDL (P<0.05 or P<0.01). The pathological changes of the liver tissues indicated that the content of lipid in the hepatocytes of betaine treatment groups was less than that in saline control group.CONCLUSION: Betaine significantly improves the lipid metabolism and the liver function in the aging db/db mice, and reduces the accumulation of lipid in the hepatocytes. 相似文献
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YUE Dong-xu ZHAO Juan-juan CHEN Hui-zi DING Tao HU Lin PAN Fei-fei GUO Meng-meng CHEN Chao XU Lin 《园艺学报》2018,34(9):1660-1665
AIM:To study the effect of adoptive transfer of CD4+ T cells with microRNA-7 (miR-7) knockdown (KD) on mouse acute liver injury model and to investigate its significance. METHODS:CD4+ CD62L+ T cells were purified from the spleen of normal wild-type (WT) mice and miR-7KD mice by magnetic bead sorting, and were stained with CFSE. These 2×106 CFSE-labeling cells were injected into normal mice via tail vein, and then the mouse acute liver injury model was induced by intraperitoneal injection of 30 mg/kg concanavalin A. After 72 h, the appearance, weight and weight index of the liver were investigated. The pathological change of the liver tissues was observed by HE staining. Real-time PCR was used to examine the mRNA expression of Bax and P53. The expression levels of CD62L, interleukin-4 (IL-4) and interferon-γ (IFN-γ) in the CD4+ T cells were analyzed by flow cytometry. RESULTS:We found that the liver tissue became lighter, and the weight (P<0.01) and weight index (P<0.05) were changed significantly in miR-7KD mice compared with control group. Moreover, HE staining showed that the liver cell damage was increased in the liver of miR-7KD mice. Meanwhile, the expression levels of Bax and P53 were significantly increased in miR-7KD group (P<0.05). The percentage of CD62L in CD4+ T cells was significantly decreased (P<0.01) in miR-7KD mice, with high expression of IFN-γ (P<0.05) and low expression of IL-4 (P<0.01) in CD4+T cells. CONCLUSION:These findings suggest that miR-7 knockdown significantly promotes the pathology of CD4+ T cell-mediated acute liver injury, which provides a preliminary experimental basis for further exploration on the mechanism of acute liver injury occurrence. 相似文献
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AIM: To analyze the expression profile of long non-coding RNA(lncRNA) in the liver tissues of drug-induced liver injury(DILI) and immune liver injury(ILI). METHODS: The technique of lncRNA microarray was used to inspect the lncRNA expression profile in the mouse liver tissues that the liver injury was induced by acetaminophen or concanavalin A. The raw data of lncRNA were pretreated for normalization. RESULTS: Compared with normal hepatic tissue, the lncRNA which had more than 1.5-fold variation and significant difference(P<0.05) by statistical analysis were regarded as lncRNA with differential expression. A total of 68 lncRNA with differential expression were found in the hepatic tissues of DILI, with 21 increased more than 1.5 folds and 47 reduced more than 1.5 folds. A total of 60 lncRNA with differential expression in the liver tissues of ILI were observed, with 17 increased more than 1.5 folds and 43 reduced more than 1.5 folds. In all lncRNA, 8 was simultaneously up-regulated in 2 liver injury models, accounting for 38% and 47% respectively, while 28 was simultaneously down-regulated in 2 liver injury models, accounting for 59% and 65% respectively. CONCLUSION: lncRNA expression profiles of DILI and ILI change significantly in comparison with normal hepatic tissue, and there are also differences between 2 hepatic damage models. The simultaneous changes of lncRNA may participate in the same or similar pathophysiological process, while the differences may be involved in relatively particular mechanisms. 相似文献
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AIM: To investigate the effect of short-term high-fructose feeding on liver triglyceride content and hepatic insulin sensitivity in mice. METHODS: Male C57BL/J6 mice were divided into control group and high (HFru) fructose group. After 3-day feeding, intraperitoneal glucose tolerance test (ipGTT) was performed to evaluate whole-body insulin sensitivity. The mice were sacrificed,and the liver samples were collected for measuring the liver triglyceride content and observing the pathological changes of the liver under light microscope with HE staining. The protein levels of lipogenic enzymes in the liver tissues were measured. To evaluate the hepatic insulin sensitivity, the protein levels (expressed as the ratio) of phosphorylated Akt/total Akt (p-Akt/t- Akt) and phosphorylated GSK-3α/β/total GSK-3α/β(p- GSK-3α/β/t- GSK-3α/β) were compared between 2 groups of the mice with or without insulin injection. RESULTS: After 3-day feeding of high-fructose diet, compared with control group, the area under the curve of ipGTT and triglyceride contents in the liver tissues were significantly increased in HFru group. HE staining of the liver in the mice in HFru group showed obvious lipid droplet formation. Compared with control group, the protein expression of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD-1) was significantly increased in HFru group. After insulin injection, the ratio of p-Akt/t-Akt and p-GSK-3α/β/t-GSK-3α/β was significantly decreased in HFru group as compared with control group. CONCLUSION: A 3-day short-term high-fructose feeding induces liver steatosis, which is related to the increased protein expression of FAS, ACC and SCD-1. Liver steatosis occurs simultaneously with the development of hepatic insulin resistance. 相似文献
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AIM: To study the effect of acute renal failure (ARF) on the differentiated frequency of Sca-1+ cells from murine fetal liver in irradiated mice. METHODS: The Sca-1+ cells from murine fetal liver were isolated with magnetic cell sorting (MACS) technique, the sex of which was identified by PCR. The 2×104 Sca-1+ cells were transplanted into a lethally irradiated (［60Co］, 8 Gy) inbred female mouse. After 8 weeks, these recipient mice were divided to A, B, and C groups at random (A group: irradiated; B group: ARF; C group: ARF and Sca-1+). The mice in B and C groups were induced to ARF with 50% (V/V) glycerin (11.6 mL/kg). 72 hours later, the mice in C group were injected with the fresh prepared Sca-1+ cells again. 8 weeks later, mice were sacrificed, and their kidneys were taken out, fixed and slices were prepared. Fluorescence in situ hybridization (FISH) of renal slices was performed and the pictures of them were taken and analyzed. RESULTS: The cells containing Y chromosome were found in renal slices from the mice in A, B and C groups, which located in epithelial cells of renal tubules, interstitium, glomeruli, and glomerular margin and increased gradually. The double and encircle zone of Y chromosome cells were found in the slices from the mice in B and C groups separately, which was consist of new renal tubules. The differentiation frequency of Sca-1+ cells in kidney in A, B and C groups were (1.65±0.18)%， (8.58±1.34)% and (18.13±1.91)%， respectively, which showed significant difference between former group and later group (P<0.01). CONCLUSION: The differentiation of Sca-1+ cells from murine fetal liver into renal cells and tissue is promoted by physiological microenvironment of acute damage and regeneration. 相似文献
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AIM: To study the effect of microRNA-7 (miR-7) knockdown (KD) on concanavalin A (ConA)-induced acute liver injury (ALI) in mice.METHODS: Wild type (WT) mice and miR-7KD mice were received ConA (30 mg/kg) to induced acute liver injury model by intraperitoneal injection, and the morphological changes, liver weight and weight index were measured 48 h later. The pathological changes of the liver tissues were observed by HE staining. The levels of serum alanine aminotransferase (ALT), IL-4 and IFN-γ were detected by ELISA. The proportional changes of CD4+ T cells and the relative levels of IL-4 and IFN-γ were analyzed by flow cytometry.RESULTS: The color of the liver tissue became lighter, and the weight and weight index were changed significantly in miR-7KD mice compared with control group (P<0.05). HE staining showed that the inflammatory cell infiltration was increased in the liver of miR-7KD mice. Moreover, the level of serum ALT was significantly increased (P<0.05). The serum level of IFN-γ elevated significantly (P<0.01), while the IL-4 levels decreased significantly (P<0.01) in the serum of miR-7KD mice. Furthermore, the proportion of CD4+ T cells and relative IFN-γ cells increased obviously (P<0.01).CONCLUSION: miR-7 knockdown promotes the pathogenesis of the ConA-induced acute liver injury in mice. 相似文献
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ZHANG Wen-jiang LIU Yuan-yue Yi-Jian ZHANG Xiao-qin HOU Ju-hua LIU Bai-yan 《园艺学报》2020,36(7):1274-1280
AIM To explore the effects of Anhua dark tea on the prevention and treatment of non-alcoholic fatty liver induced by high-fat diet in apolipoprotein E knockout (ApoE -/-) mice and the relevant mechanisms. METHODS Male ApoE -/- mice (n =50, 8 weeks old) were randomly divided into model group, atorvastatin group, and high-, medium- and low-dose Anhua dark tea groups, with 10 mice in each group. In addition, 10 homologous wild-type male C57BL/6J mice were selected as normal control group. The ApoE -/- and wild-type mice were fed with the same amount of high-fat feed and common feed for 17 weeks, respectively, and intervened by the corresponding drugs and normal saline. At the end of the experiment, HE staining was used to observe the histopathological changes of the liver. The levels of alanine aminotransfease (ALT), aspartate aminotransfease (AST), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the liver tissues were detected. The mRNA expression levels of hydroxymethyl glutaryl coenzyme A reductase (HMGCR), peroxisome proliferator-activated receptor-γ (PPAR-γ) and steroyl coenzyme A desaturase-1 （SCD-1） were detected by RT-qPCR to observe the lipid synthesis. RESULTS In model group, increased volume, smooth surface, tight membrane and dull edge of the liver were observed, and microscopic images with HE staining showed the formation of vacuoles with varying sizes, indicating the success of establishing the model. Compared with the model group, the degree of liver steatosis, the levels of ALT, AST and MDA, and the mRNA expression of HMGCR, SCD-1 and PPAR-γ in different doses of Anhua dark tea groups were significantly decreased, while the activity of SOD and GSH-Px was increased (P <0.05). The effect of Anhua dark tea in high- and medium-dose groups was better than that in low-dose group (P <0.05). CONCLUSION Anhua dark tea prevents high-fat diet-induced non-alcoholic fatty liver in ApoE -/- mice. The mechanism may be related to reducing lipid synthesis by inhibition of HMGCR, SCD-1 and PPAR-γ expression, and protecting liver cells through anti-oxidative activity. 相似文献
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AIM: To study the influence of insulin resistance on fatty liver in the mice fed with high-fat diet (HFD).METHODS: Male 8-week-old C57BL/6J mice were randomly divided into HFD group (with 60% calories by high saturated fatty acid) and control group (with chow diet).The mice in both groups were fed for 12 weeks. The body weight, liver weight, serum triglyceride (TG) and total cholesterol (TC), and blood glucose and insulin levels were measured. Hyperinsulinemic euglycemic clamp experiment was applied to reflect insulin sensitivity. The lipid deposition in the liver was analyzed by HE staining, Sudan IV staining and measurement of liver fat content. The phosphorylation levels of IRS1 and Akt, and the protein levels of SREBP-1 and FAS were determined by Western blot to reflect the activities of insulin signaling and lipid synthesis.RESULTS: Compared with control group, the body weight and liver weight were significantly increased in HFD group. TG and TC contents in serum and liver tissues were remarkably increased in HFD group. High-fat diet induced insulin resistance, as evidenced by increased serum insulin levels, reduced glucose infusion rate and decreases in IRS1 and Akt phosphorylation levels. In livers of HFD group, HE staining showed that the cytoplasm of hepatocytes was filled with vacuoles. Sudan IV staining also displayed that many different sizes of red lipid drops existed in the hepatocytes, and the protein levels of SREBP-1 and FAS were significantly increased. In primary normal hepatocytes with exogenous oleic acid intervention for 48 h, the phosphorylation levels of IRS1 and Akt were reduced, and the protein expression of SREBP-1 and FAS was significantly increased in a dose-dependent manner.CONCLUSION: Feeding with HFD leads to insulin resistance, resulting in activation of lipid synthesis and accumulation of lipid deposition in the liver, thus inducing fatty liver. 相似文献
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AIM:To assess the antioxidative activity of a new cyclic diaryheptanoid (compound 1) from ginger on free radical damage. METHODS: Hemolysis, MDA levels of mice liver tissue homogenates, the conformation changes of irradiated PUC18 were used as indices. RESULTS:Compound 1 significantly reduced the hemolysis of human red blood cells induced by H2O2 (P＜0.01) and MDA level in the liver of mice (P＜0.01), raised the percentage of the supercoil conformation of plasmid PUC18 induced by[60Co]γ-rays (P＜0.05 or P＜0.01).CONCLUSION:Compound 1 had a good activity on suppressing oxygen free radical damage. 相似文献