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1.
The identification and study of adaptively important genes in forest trees represents a formidable challenge because of their long generation spans. In annual or perennial herbs, formal genetic studies can be employed to identify the quantitative trait loci (QTLs) and/or candidate genes that underlie important traits, and the segregating populations can be transplanted into natural populations to measure the strength and direction of selection. However, the application of these methods to forest trees is difficult, because the creation of appropriate genetic material is extremely time-consuming in long-lived, woody plants, and lifetime fitness estimates are difficult or impossible to obtain. Although QTL mapping should in principle be feasible in wild intraspecific populations (as an alternative to artificial crosses), this approach is less likely to be successful in trees because LD (linkage disequilibrium) will decay quickly in large outbreeding plant populations. Within the present paper, we discuss a modified approach based on natural hybrid zones. We describe the use of wild annual sunflowers (Helianthus spp.) as a model for exploring the hybrid zone approach. Transplanted experimental hybrids allowed us to assess the adaptive value of individual chromosomal blocks in nature, and data on natural Helianthus hybrids suggest that similar approaches are possible in natural hybrid zones. Our results allowed us to test the role of hybridization in the origin of ecological divergence in wild sunflowers. In addition, they have practical implications for identifying adaptively important genes or QTLs in trees. This is exemplified by three temperate forest taxa, Populus (poplars, aspens, cottonwoods), Fraxinus (ash), and Quercus (oak). All three are diploid and important genomic tools are under development. Moreover, all three offer extensive hybrid zones whose likely age can be inferred from fossil data. Age data enables estimates of the size and frequency of chromosomal blocks in hybrids, thereby providing guidance in designing marker-based experiments. We predict that natural hybrid zones will be valuable tools for identifying the QTLs and/or candidate genes responsible for adaptive traits in forest trees. 相似文献
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Use of DNA markers in forest tree improvement research 总被引：4，自引：0，他引：4
D. B. Neale M. E. Devey K. D. Jermstad M. R. Ahuja M. C. Alosi K. A. Marshall 《New Forests》1992,6(1-4):391-407
DNA markers are rapidly being developed for forest trees. The most important markers are restriction fragment length polymorphisms (RFLPs), polymerase chain reaction- (PCR) based markers such as random amplified polymorphic DNA (RAPD), and fingerprinting markers. DNA markers can supplement isozyme markers for monitoring tree improvement activities such as; estimating genetic diversity in breeding populations, germplasm identification, verifying controlled crosses, and estimating seed orchard efficiencies. Because the number of DNA markers is potentially limitless, it should be possible to map individual quantitative trait loci (QTL) by linkage analysis with high-density maps. Finally, if such associations can be found, it may also be possible to design marker-assisted breeding strategies for forest trees. 相似文献
4.
James W. Hanover 《New Forests》1992,6(1-4):159-178
Terpenoid substances in forest trees are versatile biochemical systems for use as genetic markers and for studying genetic regulation at the biochemical level. Univariate, multivariate and correlative data analyses from numerous studies on different coniferous species are presented. These data indicate that fitness values for most terpenes examined are probably so low as to be unmeasurable except in long evolutionary time spans. Variation patterns for monoterpenes often closely parallel racial differentiation for morphological traits and reflect the migratory history of species and species-complexes. Random drift and gene flow are likely the major influences on genetic organization of terpene variability. Mutation in regulatory genes may be responsible for quantitative variation within major gene systems for terpenes. The mono-, sesqui- and diterpene systems in conifers offer good opportunities for research on the nature of gene regulation in plants and its evolutionary significance. 相似文献
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分子标记辅助选择在林木育种中的应用 总被引：8，自引：0，他引：8
历史上,数量遗传学被认为是指导林木育种的唯一有效工具,这有两个方面的原因：第一,在林木上具有重要经济价值的性状,如树干材积生长、木材纤维长度及适应性等,均是以数量方式遗传的性状,并且控制这些性状的每一个过程是假设受微效多基因所控制的;第二,林木具有很长的世代间隔,很高的遗传杂合性和很高的遗传负荷,这些特性限制了一些经典的遗传材料,如近交系,突变体及特异类型的产生。然而,传统的数量遗传学方法用于指导林木育种的实践并不是完美无缺的。林木个体高大,占地面积大,且大多栽植在非农用耕地上,这样的环境异质性在很大程度上影响性状遗传力的提高,有些林木树种人工杂交比较困难,很难获得足够大的家系,从而产生不平衡的交配设计。Ｎａｍｋｏｏｎｇ 和Ｒｏｂｅｒｄｓ（１９７６） 认为,不平衡的交配设计很难获得准确的遗传参数估计。到目前为止,大多数成功的林木育种方案往往都是基于对表型的简单选择。这种表型选择不能利用林木固有的非加性效应,以及对异质环境的反应潜能,因而育种效果是极为有限的。分子标记技术为现代数量遗传学的发展提供了新的方向。通过利用中性标记与影响数量性状的位点（ＱＴＬ） 之间的连锁关系,人们已经提出了一套统计方法用来鉴定重要的Ｑ 相似文献
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Diana Krajmerová Matúš Hrivnák Ľubica Ditmarová Gabriela Jamnická Jaroslav Kmeť Daniel Kurjak Dušan Gömöry 《New Forests》2017,48(3):463-477
Although European beech (Fagus sylvatica L.) is one of the most widespread and ecologically and commercially most important deciduous trees in Europe, little is known about its adaptive genetic variation. We explored single-nucleotide polymorphism (SNP) variation in candidate genes for budburst and drought resistance in beech populations sampled across most of the distribution range, represented in an international provenance trial. SNP variation was monitored for six candidate genes, in 114 individuals from 19 natural populations. Population structure was deduced from the analysis of 20 nuclear microsatellite markers. Different methods to detect imprints of natural selection were used (F ST-outlier, SNP-climate regression, association tests). The F ST-outlier approach identified the COV gene with unambiguous signal of selection, which is an orthologue of Arabidopsis gene for a membrane protein previously reported as phenology-related. Based on environmental association analysis at the population level, the dehydrin gene was found associated with drought-related climatic variables. At the individual level, dehydrin gene also showed a significant association with chlorophyll fluorescence parameters, which are considered stress markers. The importance of the knowledge of physiological variation and geographical patterns of adaptive genetic variation for guiding reproductive materials transfer under climate change is stressed. 相似文献
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A total of 24 candidate plus trees (CPTs) of Pongamia pinnata (L.) Pierre. were selected to elucidate their variation and diversity based on thirteen quantitative traits (4 pod traits,
6 seed traits of parent trees and 3 progeny traits) at Forest Research Centre, Institute of Forest Productivity — Mandar,
Ranchi district during 2005–2007. The results show that, CPT-19 had maximum for seven traits viz, pod length (65.6 mm), 100-pod
weight (542.4 g), seed 2D (two dimension) area (351.2 mm2), seed length (27.9 mm), seed breadth (17.4 mm), 100-seed weight (217.9 g) and plant height (164.3 cm). The traits, 100-pod
weight and 100-seed weight had a high heritability (98.4%, 96.9%) accompanied with high genetic advance (46.0%, 34.9%). There
is a positive significant correlation between 100-pod weight and 100-seed weight traits at both genotypic and phenotypic levels
with plant height, collar diameter and volume index at 30 MAS (months after sowing). Volume index expressed a moderate heritability
(47.4%) accompanied with high genetic advance (48.4%), indicating that the character is governed by additive gene effects.
In divergence study, 24 accessions were grouped into 6 clusters on the basis of non-hierarchical euclidian cluster analysis.
The genotypes in cluster IV (CPT-5, CPT-6, CPT-7, CPT-12, CPT-16, CPT-18, CPT-22) and cluster III (CPT-4, CPT-8, CPT-9, CPT-20,
CPT-21) were most heterogeneous and can be best used within group hybridization. The wide diversity exists between the cluster
V and II, followed by cluster II and I and crosses between CPTs of these clusters may result in substantial segregates. It
is revealed that the existence of substantial variation and diversity can be utilized for genetic resource conservation and
further tree improvement programmers of the species. 相似文献
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中国杨树分子遗传改良的研究现状 总被引：1，自引：0，他引：1
杨树是中国广泛栽培的重要造林树种之一 ,我国是世界上杨树资源丰富的国家 .杨树具有速生丰产、实用性强、无性繁殖能力强 ,且基因组较小等特点 ,现已成为研究林木生理和基因工程研究的模式树种 .该文概述了基因工程技术、遗传图谱构建、重要性状基因定位以及分子标记辅助选择育种等方面在中国杨树遗传改良中的应用研究进展 ,并对现代分子生物学技术在林木遗传改良应用中存在的主要问题和应用前景进行了讨论 相似文献
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分子标记在林业辅助选择育种中的应用 总被引：4，自引：0，他引：4
本文主要阐述分子标记在林木辅助选择育种中的应用。利用多种分子标记(RAPD,RFLP,AFLP,STS,SSR,STR等),可以在林木早期生长阶段对一些性状进行鉴别, 构建单种分子标记遗传连锁图谱或几种分子标记共存的混合连锁图谱和对控制数量性状的基因进行定位, 对林木群体遗传结构、遗传变异、遗传分化和基因流动进行研究, 在基因工程中, 能够追踪目的基因行为和对控制质量性状的基因进行鉴别, 对单株进行指纹图谱, 对种子质量进行监测和对品系、品种和无性系进行鉴定。 相似文献
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通过对孟家岗林场40年生樟子松母树林不同密度疏伐处理后14个主要性状的统计分析,结果表明,疏伐处理对樟子松母树多个性状造成了显著影响,而且不同性状之间存在极为复杂的相关性;利用聚类分析和主分量分析法将存在显著差异性和相关性的8个性状分成3类,分别代表了生长、树冠和结实性状;通过这3类性状在各主成分上得分值的加权和比较表明,孟家岗林场40年生樟子松母树林疏伐抚育最佳密度是150株·hm-2。 相似文献
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林木遗传育种基础研究热点述评 总被引：1，自引：0，他引：1
林木由于世代周期长、个体高大、遗传负荷高等自身固有的一些特性,一直被认为是一种非常难以操作的遗传学材料,导致林木遗传育种在基础研究方面远远滞后于模式动、植物和其他重要作物,成为限制林木遗传改良进程的主要因素。分子育种是突破林木育种周期长的关键技术,通过基因组学研究,分析和阐明林木基因的功能是进行林木分子育种设计的前提。近年来随着生命科学领域新技术的快速发展,林木遗传育种基础研究也出现一些新的热点。本文主要阐述林木基因组和功能基因组学、连锁与关联分析、木材形成机制及分子育种技术的研究进展,虽然目前相关研究成果实际应用的条件还不成熟,但作为技术储备是急需加强的研究方向。 相似文献
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林木重要性状的杂种优势和遗传方式以及杂优预测的可能性 总被引：1，自引：0，他引：1
林木杂交育种成功的先决条件, 取决于杂种具有的正向杂种优势是否明显, 因此林木杂种优势的机理研究十分重要。一般来讲, 林木重要性状的杂种优势产生的机理应和该性状的遗传控制方式一致, 但是林木重要性状的遗传控制方式取决于交配群体的遗传结构, 并和多种因素有关, 因而不应随便地根据几个随机亲本的配合力分析结果, 就断然肯定该树种杂交是否有前途。与以往更多地重视种间杂交的观点不同, 文中认为林木的种内杂交在林木多性状改良中也有着巨大的潜力, 并将发挥重要作用。最后根据林木杂种优势与配合力间的联系, 就其对林木杂优预测的影响提出了笔者的认识。 相似文献
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Sapindus mukorossi Gaertn. and S. delavayi Franchet are among the most valuable species in the genus Sapindus for their commercially exploitable plant oils and chemicals. However, few studies have addressed genetic variation and improvement for either species. We evaluated the genetic diversity of germplasm from selected plus trees within a wide region and established the relationship between fruit traits and molecular markers. An association analysis based on inter-simple sequence repeats(ISSRs)provided a genetic basis for studies of fruit traits. A total of 247 loci were detected by scanning 61 trees of S. mukorossi and S. delavayi using 16 ISSR markers. Genetic diversity parameters were estimated for selected superior trees(or germplasm) and S. mukorossi and S. delavayi were categorized into two main groups, as well as into four groups within S. mukorossi. An association analysis between the ISSR markers and 14 fruit traits used the TASSEL MLM model. A genetic structure analysis differentiated S.mukorossi and S. delavayi. Eighteen ISSR loci associated with 13 fruit traits(P<0.005) were identified, with 13, 1,and 4 loci associated with seed oil production, fruit saponin production, and fruit quality, respectively. Using this information, a core collection was selected with adequate genetic diversity and good seed oil characters. Our results demonstrate the feasibility of effectively estimating fruit trait associations in Sapindus using ISSR markers, and the method is applicable and valuable for select germplasm conservation. The markers obtained in this study are potentially useful for molecular-assisted breeding of Sapindus spp. 相似文献
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A. Dounavi N. Koutsias M. Ziehe H. H. Hattemer 《European Journal of Forest Research》2010,129(6):1191-1202
Analyses of distribution patterns and genetic structures of forest stands can address distinct family structures and provide
insights into the association of genetic and phenotypic variation patterns. In this study, point pattern analysis and spatial
autocorrelation were used to examine the spatial and genetic structures in two naturally generated beech stands, which differ
in age, trunk morphology, and stand management. Significant tree clumping was observed at distances up to 20 m in the young
forest stand, whereas dispersion at distances under 10 m was observed in the old stand. The spatial analysis based on Ripley’s k function of the two different groups of trees showed that the non-forked trees match in both stands the spatial pattern of
all trees while the forked were randomly distributed. Additionally, according to the bivariate analysis, forked trees in both
stands were randomly distributed as related to non-forked tree positions. Finally, Moran’s I values were not very high, though significant genetic autocorrelation was identified at distances up to 20 m in the young
stand, suggesting the existence of distinct family structures. However, no significant genetic structuring was observed in
the old stand. Our findings suggest that spatial genetic patterns are impacted by stand age, environmental factors and human
activities. The spatial distribution of forked trees was not clearly associated to family structures. Random effects and also
micro-environmental variation could be additional factors explaining forking of beech individuals. 相似文献
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The recent completion of a draft sequence of the poplar (Populus trichocarpa Torr. & Gray ex Brayshaw) genome has advanced forest tree genetics to an unprecedented level. A "parts list" for a forest tree has been produced, opening up new opportunities for dissecting the interworkings of tree growth and development. In the relatively near future we can anticipate additional reference genome sequences, including the much larger Pinus genome. One goal is to use this information to define the genomic attributes that affect the phenotypic performances of trees growing in various environments. A first step is the definition of ideotypes that constitute optimal tree and stand-level performance. Following this, the genome can be systematically searched for genetic elements and their allelic variants that affect the specified traits. Knowledge of these alleles and their effects will facilitate the development of efficient tree improvement programs through genome-guided breeding and genetic engineering and further our mechanistic understanding of trait variation. Improved mechanistic understanding of tree growth and development is needed to develop process models that will allow us to anticipate and manage change in forest ecosystems. Here we consider the development of an ideotype for loblolly pine (Pinus taeda L.) and discuss genomic approaches for studying the component traits that will enable advances in process model development and the genetic improvement of this important conifer. 相似文献
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[目的]建立一套适合印楝药用优树无性系测定的分析评价方法,并进行农药型印楝优株评价。[方法]以印楝药用优树和平均木无性系为试验材料,通过不完全谱系设计建立无性系品比试验,采用重复力估算法在表型水平上对无性系当代种子农药品质性状进行遗传变异分析,提出"农药型印楝"的概念和标准,根据该标准进行农药型印楝优株评价。[结果]无性系种子印楝素组分含量随优树种子印楝素组分含量的变化而变化,但在无性系内和无性系间都存在不同程度变异:同一无性系各分株间的印楝素B变异(5.26%37.55%)较印楝素A变异(6.06%15.80%)和印楝素AB变异(2.95%16.14%)大,印楝素B和印楝素AB几乎呈退化和/或趋中遗传倾向,而印楝素A则既有退化和/或趋中遗传倾向又有超母遗传倾向;无性系间种子印楝素组分含量性状具有异质性(P0.01)。无性系印楝素A重复力(0.78)、印楝素B重复力(0.81)和印楝素AB重复力(0.83)均较高,表明无性系间印楝素组分含量的差异主要是由遗传效应和一般环境效应造成的,受特殊环境效应影响较小。按农药型印楝标准,评定LD0505、WW0401、AT0515三个无性系为"农药型印楝品种",LD0505和WW0421是"农药型印楝亲本"。[结论]重复力估算法适用于印楝药用优树无性系测定的分析评价,优树种子农药品质性状的遗传传递力强,无性系能保持优树农药品质的优良特性;评定LD0505、WW0401、AT0515为农药型印楝品种,LD0505和WW0421还可作为农药型印楝亲本,其余优树无性系不宜选择。 相似文献
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地基激光雷达在森林参数反演中的应用 总被引：2，自引：1，他引：1
近年来地基激光雷达(TLS)得到了快速发展, 它能够快速地获取高精度、高密度的3D点云数据, 可以应用到工程设计、林业、农业、矿产、交通、城市规划和遗产记录等领域。在森林参数的定量测量应用上, 利用TLS测量森林参数如树高、胸径、密度和蓄积量等, 不仅节省人力, 还提高了工作效率, 现在已经成为一种快速获取树木几何参数的有效方法。文中主要介绍地基激光雷达遥感的工作原理、数据处理过程和方法、在林业中的应用现状、利用TLS数据反演森林参数(位置、胸径、树高、蓄积量、树干和郁闭度等)的方法和研究进展, 同时对地基激光雷达在林业中的应用前景进行了分析和展望。 相似文献
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松树是世界上森林生态系统和人工林的重要树种,松树的遗传改良开展早,进展快,成效大.分子标记技术为缩短育种周期,提高育种效率提供了有力的工具.本文回顾了世界上松树分子标记遗传图谱构建、比较遗传作图、数量性状位点定位和标记辅助选择的研究进展.已经构建遗传连锁图的林木有13个属,近40个树种,其中松树占40%,而大多数松树遗传图谱仍然是不完整的,不能覆盖全基因组;比较遗传作图显示松属树种具有高度的遗传保守性;数量性状位点(QTL)定位表明,大多数性状的遗传基础存在着主效基因,为开展分子标记辅助选择提供了良好的基础.杂种松部分重要性状的遗传控制中存在着树种效应,对标记辅助选择和育种策略的制订具有指导意义. 相似文献