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1.
Sasikumar JM  Doss PA 《Fitoterapia》2006,77(7-8):605-607
Methanol extract of Rhaphidophora pertusa stem was analyzed for its antioxidant (DPPH, reducing power and Fe(3+) metal chelation methods) and antibacterial activities. The extract was found effective against the three antioxidant test models and exhibited strong and moderate antibacterial activity against the tested pathogenic bacteria.  相似文献   

2.
The hydroalcoholic extract of Maytenus krukovii bark was investigated for its in vitro mutageno-protective activities by means of the Ames Salmonella/microsome assay. The extract showed an inhibitory effect in both T98 and T100 strains against the mutagenic activity of promutagen 2-aminoanthracene but was not protective against directly acting mutagens sodium azide and 2-nitrofluorene. When tested as a radical scavenger and antioxidant it produced a dose-dependent inhibition. The extract did not show significant antibacterial properties, and was weakly active against dermatophyte and phytopathogenic fungi, but inhibited the growth of phytopathogen Pithyum ultimum.  相似文献   

3.
The n-hexane-dichloromethane (1:1) extract of the roots of Onosma argentatum and the methanol extract (partitioned between water and chloroform, ethyl acetate and n-butanol, respectively), of the underground parts (roots and rhizomes) of Rubia peregrina were tested in vitro for their antioxidant and antimicrobial activities. The highest antioxidant activity (98%) was observed at 0.1% concentration for the roots of O. argentatum. It was 96% at 0.25% concentration on the ethyl acetate fraction of R. peregrina. O. argentatum extract was effective on Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The ethyl acetate and chloroform fractions of R. peregrina were effective on S. aureus and E. coli, respectively. These two species did not have any antifungal activity.  相似文献   

4.
Amanlou M  Fazeli MR  Arvin A  Amin HG  Farsam H 《Fitoterapia》2004,75(7-8):768-770
The methanolic extract of the aerial parts of Satureja khuzistanica was investigated for its antimicrobial activity. The maximum antibacterial and antifungal activities were observed against Staphylococcus aureus and Candida albicans.  相似文献   

5.
Ay M  Bahadori F  Oztürk M  Kolak U  Topçu G 《Fitoterapia》2007,78(7-8):571-573
The antioxidant properties of the methanol extract of leaves and flowers of Erica arborea and the ethyl acetate, butanol and water soluble fractions were investigated. The ethyl acetate extract was found to be the richest for phenolic and flavonoid content which showed the highest antioxidant activity.  相似文献   

6.
Pleurotus citrinopileatus Singer has recently become a popular delicacy in East Asian countries. We prepared a methanol extract, soluble fractions from the methanol extract, and a hot water extract of the fruiting bodies of P. citrinopileatus. The biological activities such as melanin biosynthesis inhibition, tyrosinase inhibition, antioxidant activities [1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC), and superoxide dismutase (SOD)-like activity], antibacterial activities, and antihyaluronidase activities of these extracts were evaluated. We found that the n-hexanesoluble, diethyl ether-soluble, and ethyl acetate-soluble fractions exhibited melanin biosynthesis inhibition in B16 melanoma cells, as well as antioxidant (ORAC) and antibacterial activities. However, the n-butanol-soluble and water-soluble fractions and the methanol and hot water extracts exhibited antioxidant (DPPH radical scavenging, SOD-like activity) and antihyaluronidase activities. These results indicate that the fruiting bodies of P. citrinopileatus have the potential to be used as an ingredient in skin cosmetics.  相似文献   

7.
为给鸭儿芹的综合利用提供科学依据,以鸭儿芹茎、叶为原料,采用水蒸气蒸馏法提取鸭儿芹精油,利用气相色谱-质谱联用技术(GC-MS)对鸭儿芹精油成分进行了分析,利用DPPH、ABTS自由基清除能力试验与FRAP试验及打孔法抑菌试验对其精油的抗氧化抗菌活性进行了分析。结果表明:(1)精油成分方面,从鸭儿芹精油中共分离出32个峰,占总离子峰的93.75%,共鉴定出30种化合物,其主要成分分别为α-芹子烯(42.01%)、β-芹子烯(19.86%)、(Z)-β-金合欢烯(12.76%)、镰叶芹醇(4.16%)、α-没药醇(3.93%)等;(2)抗氧化能力方面,当鸭儿芹精油浓度由19μg/m L上升到38μg/m L时,DPPH反应液中Trolox的当量由0.003μmol上升到0.007μmol,ABTS反应液中Trolox的当量由0.069μmol上升到0.074μmol,FRAP反应液中Trolox的当量由0.225μmol上升到0.280μmol,当精油浓度在一定范围内,其DPPH清除能力、ABTS自由基清除能力及铁离子还原抗氧化能力与其浓度间均呈量效关系,且均呈正相关效应,表明鸭儿芹精油具有良好的抗氧化能力;(3)抗菌活性方面,当鸭儿芹精油浓度为1 280 mg/m L时,金黄色葡萄球菌的抑菌圈直径可达7.05 mm,表现出一定的抑菌活性,且其对金黄色葡萄球菌的抑菌效果显著优于其对大肠杆菌的抑菌效果。  相似文献   

8.
Rahman MS  Sadhu SK  Hasan CM 《Fitoterapia》2007,78(7-8):552-555
The ethanolic extract of Leucas aspera root was subjected to acetic acid induced writhing inhibition, 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay and brine shrimp lethality bioassay for screening of antinociceptive, antioxidant and cytotoxic activity, respectively. The extract produced significant writhing inhibition in acetic acid induced writhing in mice at the doses of 250 and 500 mg/kg. The extract showed a significant free radical scavenging activity with an IC(50) of 8 microg/ml. The extract showed significant lethality to brine shrimp with an LC(50) value.  相似文献   

9.
Indonesian medicinal plants were screened as potential sources of antiacne agents. The screening methods were performed using antibacterial assay against Propionibacterium acnes, lipase inhibitor assay, and antioxidant assay. The results showed that from 40 plant materials extracted with methanol and 50% ethanol in water, Caesalpinia sappan was the best extract based on the combined activities: antibacterial (minimum inhibitory concentration 0.13 mg/ml; minimum bactericidal concentration 0.25 mg/ml), lipase inhibitory [50% inhibitory concentration (IC50) 120.0 μg/ml], and antioxidative (IC50 6.47 μg/ml). Another prospective extract is Intsia palembanica based on its lipase inhibitory activity (IC50 4.1 μg/ml) and antioxidant activity (IC50 3.87 μg/ml). Part of this report was presented at the 58th Annual Meeting of the Japan Wood Research Society, Tsukuba, March 2008  相似文献   

10.
Russo A  Longo R  Vanella A 《Fitoterapia》2002,73(Z1):S21-S29
Propolis, a natural product produced by the honeybee, has been used for thousands of years in folk medicine for several purposes. The extract contains amino acids, phenolic acids, phenolic acid esters, flavonoids, cinnamic acid, terpenes and caffeic acid. It possesses several biological activities such as antiinflammatory, immunostimulatory, antiviral and antibacterial. The exact mode of physiological or biochemical mechanisms responsible for the medical effects, however, is yet to be determined. In this work, we have investigated the antioxidant activity of a propolis extract deprived of caffeic acid phenethyl ester (CAPE). In addition, the activity of CAPE and galangin was also examined. Propolis extract (with and without CAPE) and its active components showed a dose-dependent free radical scavenging effect, a significant inhibition of xanthine oxidase activity, and an antilipoperoxidative capacity. Propolis extract with CAPE was more active than propolis extract without CAPE. CAPE, used alone, exhibited a strong antioxidant activity, higher than galangin. The experimental evidence, therefore, suggests that CAPE plays an important role in the antioxidant activity of propolis.  相似文献   

11.
Onay-Uçar E  Karagöz A  Arda N 《Fitoterapia》2006,77(7-8):556-560
Methanolic extracts of Viscum album ssp. album (mistletoe) grown on different host trees were investigated for their potential antioxidant activity. Scavenging activity was tested by 1,1­diphenyl­2­picrylhydrazyl (DPPH) method and the inhibitory effect on lipid peroxidation was examined by ferric thiocyanate and thiobarbituric acid methods. The extract from mistletoe grown on lime tree in summer showed the highest activity. It was found that antioxidant capacity of the plant differed according to the harvesting time as well as the host tree.  相似文献   

12.
Contents of bioactive components, free radical scavenging and anti-acne producing bacteria activities of young and mature fruit rind extracts of mangosteen were compared. The young fruit rind extract contained significantly higher contents of phenolics and tannins and promoted higher free radical scavenging activity than the mature fruit rind extract, while the later extract contained higher contents of flavonoids and α-mangostin xanthone and gave higher anti-acne producing bacteria activity than the young fruit rind extract. Thus, the young and mature stages of mangosteen fruit rind should be beneficial for further development of antioxidant and anti-acne pharmaceutical preparations, respectively.  相似文献   

13.
The methanol extract of Leucas aspera flowers, its fractions, the alkaloidal residue and the expressed flower juice, tested for antimicrobial activity, showed good antibacterial activity for methanol extract and methanol fraction with maximum activity for the alkaloidal residue.  相似文献   

14.
Calocedrus formosana Florin (Cupressaceae) is an endemic tree to Taiwan. To evaluate the antioxidant activity of the plant extracts from heartwood, bark, and leaf of C. formosana, assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion scavenging activities, as well as prevention of DNA strand cleavage were performed in this study. Similar IC50 values against the DPPH radical were found for the heartwood and bark extracts at approximately 23µg/ml. Moreover, the heartwood extract exhibited the highest inhibitory activity against superoxide radicals among the test samples; a 2.3-fold lower value of IC50 for superoxide radical inhibition was found in the heartwood extract relative to that of (+)-catechin. Much less effect on inhibition of DPPH and superoxide radicals was found from the leaf extract of C. formosana. More than 70% of superoxide radicals were inhibited in the presence of 10µg/ml heartwood extract, whereas only 15% inhibition was obtained from the leaf extract. The heartwood extract, at a dose of approximately 0.5mg/ml, apparently completely prevented the X174 supercoiled DNA cleavage induced by ultraviolet photolysis of H2O2, as judged by agarose gel electrophoresis. This report also suggests that the antioxidant activities of the plant extracts of C. formosana are in good correlation with their phenolic contents.  相似文献   

15.
The scavenger activities of Equisetum arvense, Equisetum ramosissimum and Equisetum telmateia aboveground parts phosphate buffer (pH 7) extracts were evaluated using three different methods: DPPH assay, ESR and NO radical inhibition assay. Total reducing power was determined by FRAP assay. The E. telmateia extract demonstrated the most relevant scavenger and antioxidant properties. ESR signal of DMPO-OH radical adducts in the presence of E. telmateia phosphate buffer (pH 7) extract was reduced to 98.9% which indicated that E. telmateia could be a useful source of antioxidants with huge scavenger ability.  相似文献   

16.
以广东紫珠药材粉末为原料,探讨其不同提取部位的抑菌作用,并筛选其有效部位。对广东紫珠药材用不同极性大小溶剂（石油醚、二氯甲烷、乙酸乙酯、乙醇、70%乙醇、水）回流提取,获得广东紫珠粗提物,用滤纸片法测定其抑菌圈大小（金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌、白色念珠菌）,对抑菌活性明显的提取部位进行梯度萃取,并分别测定其抑菌圈大小,得到广东紫珠抑菌有效部位,并测定其有抑菌作用的有效部位的最小抑菌浓度。结果显示广东紫珠70%乙醇提取物对金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌均表现了不同程度的抑菌活性,初步判定为广东紫珠的抑菌活性部位,70%广东紫珠提取物经过萃取后,石油醚萃取物及二氯甲烷萃取物没有抑菌作用,乙酸乙酯萃取物、正丁醇萃取物及剩余的水部分均有不同程度的抑菌作用。70%乙醇广东紫珠提取物通过二氯甲烷萃取除去部分脂溶性杂质,即得到广东紫珠抑菌部位,可以此为基础寻找广东紫珠抑菌活性成分,为后期开发利用提供理论依据。  相似文献   

17.
Antioxidant activity of Litsea cubeba   总被引：5，自引：0，他引：5  
Hwang JK  Choi EM  Lee JH 《Fitoterapia》2005,76(7-8):684-686
The antioxidant activity of Litsea cubeba was studied in terms of three different assay systems: DPPH assay, peroxidase/guaiacol assay, and TBA test. The L. cubeba methanol extract and its fractions showed remarkable antioxidant activity in comparison with alpha-tocopherol and ascorbic acid.  相似文献   

18.
Antibacterial activity of Cichorium intybus   总被引：1，自引：0，他引：1  
Antibacterial activity of the water, ethanol and ethyl acetate extracts of Cichorium intybus was investigated. All the tested extracts showed antibacterial activity, the ethyl acetate extract being the most active. Water extract inhibits Agrobacterium radiobacter sp. tumefaciens, Erwinia carotovora, Pseudomonas fluorescens and P. aeruginosa.  相似文献   

19.
The methanolic extract of the root of Garcinia multiflora and its derived soluble fractions, namely those soluble in ethyl acetate (EtOAc), n-butanol, and water, were screened for their antioxidant activities. Among them, the EtOAc-soluble fraction exhibited the highest scavenging activity against the 1,1-dipheny1,2-picrylhydrazyl radical, the highest superoxide radical scavenging activity, and the strongest reducing power. In addition, according to the bioactivity-guided isolation, 26 specific phytocompounds, including 3 aromatics, 3 benzophenones, 3 flavonoids, 3 isocoumarins, 1 phloroglucinol, 6 steroids, and 7 xanthones, were isolated from the EtOAc-soluble fraction and identified. Of these, 2,4,3′,4′-tetrahydroxy-6-methoxybenzophenone and 1,3,6,7-tetrahydroxyxanthone were found to be the major bioactive constituents, present in the crude extract in concentrations of 3.9 and 15.6 mg/g, respectively. These two compounds had similar antioxidant activities to (+)-catechin, a well-known antioxidant.  相似文献   

20.
The antioxidant activity of the methanol extract of Filipendula hexapetala flowers was assessed by the assay for ferric-reducing antioxidant power (FRAP), the assay for DPPH free radical scavenging ability (DPPH) and the assay for the influence of lipid peroxidation in liposomes, induced by Fe(2+)/ascorbate system and measured by the TBA test (LP). The activity of the investigated extract in all test-systems was found to be significant. The principal constituent responsible for the observed effects was isolated and identified as spiraeoside.  相似文献   

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