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1.
Cool white fluorescent (CWF) light reduces Fe3+ to Fe2+ while low pressure sodium (LPS) light does not. Cotton plants grown under CWF light are green, while those yrown under LPS light develop a chlorosis very similar to the chlorosis that develops when the plants are deficient in iron (Fe). It could be that CWF light (which has ultra violet) makes iron more available for plant use by maintaining more Fe2+ in the plant. Two of the factors commonly induced by Fe‐stress in dicotyledonous plants‐‐hydroyen ions and reductants released by the roots‐‐were measured as indicators of the Fe‐deficiency stress response mechanism in M8 cotton.

The plants were grown under LPS and CWF light in nutrient solutions containing either NO3‐N or NH4‐N as the source of nitrogen, and also in a fertilized alkaline soil. Leaf chlorophyll concentration varied significantly in plants grown under the two light sources as follows: CWF+Fe > LPS+Fe > CWF‐Fe ≥ LPS‐Fe. The leaf nitrate and root Fe concentrations were significantly greater and leaf Fe was generally lower in plants grown under LPS than CWF light. Hydrogen ions were extruded by Fe‐deficiency stressed roots grown under either LPS or CWF light, but “reductants”; were extruded only by the plants grown under CWF light. In tests demonstrating the ability of light to reduce Fe3+ to Fe2+ in solutions, enough ultra violet penetrated the chlorotic leaf of LPS yrown plants to reduce some Fe3+ in a beaker below, but no reduction was evident through a yreen CWF grown leaf.

The chlorosis that developed in these cotton plants appeared to be induced by a response to the source of liyht and not by the fertilizer added. It seems possible that ultra violet liyht could affect the reduction of Fe3+ to Fe2+ in leaves and thus control the availability of this iron to biological systems requiring iron in the plant.  相似文献   

2.
In a companion paper (10), varieties of four plant species [two monocotyledons (oats and corn) and two dicotyledons (soybeans and tomato)] were shown to differ widely in their ability to respond to Fe‐stress. The ability of the more Fe‐efficient varieties was manifested by a lowering of the pH of the ambient medium of the root and/or by loss of reductants from the root. Both effects can enhance uptake of Fe by the roots, since Fe is taken up primarily, if not entirely, as Fe2+ ions. Thus, a given stressed plant has a means, under some degree of metabolic control, for modifying the root environment and, thereby, alleviating its chlorotic condition.

The present investigation deals with environmental factors, particularly chemical inhibitors, modifying the effectiveness of the stress response. Without inhibitors, excised root samples of the four species exhibited a wide range of abilities to reduce Fe3+ to Fe2+. Roots of the dicotyledonous species reduced about twice as much Fe3+ as did equal weights of the monocotyledonous species. Iron‐efficient tomato, soybean, and oat roots reduced more Fe3+ than did roots of the Fe‐inefficient varieties. The two corn varieties were about equal in their effectiveness.

Comparable samples of roots were also exposed to chemicals that induce or aggravate Fe chlorosis. Those found to be very effective inhibitors of Fe3+ reduction by the roots included: hydroxide, orthophosphate, pyrophosphate, Cu2+ and Ni2+. Other ions (includ ing Mn2+, Zn2+ and molybdate) and ethyl ammonium phosphate also inhibited Fe3+ reduction but to a lesser degree. Citrate, however, enhanced Fe3+ reduction. The degree of inhibition or enhancement differed for each of the varieties. In general, the Fe‐efficient plants were best able to reduce Fe3+ in spite of the inhibitory influence of the imposed treatments. Thus, our findings indicated that inhibition of the Fe3+ ‐reduction process at, or near, the periphery of the root is an apparent cause of Fe chlorosis.  相似文献   

3.
Cotton (Gossypium hirsutum L.) plants grown under low pressure sodium lamps (LPS) developed chlorosis which was similar in appearance to iron‐stress induced chlorosis, while plants under cool white fluorescent lamps (CWF) at the same level of photosynthetically active radiation (PAR) developed normally. These illumination sources differ in spectral irradiance; CWF lamps emit ultra violet (UV), whereas LPS lamps do not. Ultraviolet radiation is capable of reducing Fe3+ to Fe2+ through a chlorotic leaf which may be important in establishing an active iron fraction in the leaf. Root reduction of Fe3+ to Fe2+ was lacking in Fe‐stressed cotton under LPS light, but was present under CWF light. Net photosynthesis, photosynthetic electron transport, and leaf chlorophyll content were lower under LPS than CWF light in most of the growing media studies (soil or solutions with nitrate‐ or ammonium‐nitrogen supplied). Chloroplast ultrastructure and leaf thickness were also altered by LPS irradiance. Electron microscopic studies with plants grown in nutrient solutions for 4 weeks suggested that chioroplastic granal disorganization was more directly associated with diminished iron supplies than with light source. However, plants grown in soil for 6 weeks under LPS light had granal disorganization similar to that found in iron‐stressed plants. These studies suggest an important role for UV radiation in influencing the activity of iron in plants.  相似文献   

4.
Abstract

Iron (Fe) chlorosis is a major nutritional constraint to groundnut (Arachis hypogaea L.) productivity in many parts of the world. On‐farm research was conducted at a Fe‐chlorotic site to evaluate the performance of three genotypes (TMV‐2, ICGS‐11, and ICGV‐86031), three fertilizer practices [no fertilizer control, fanners practice (125: 200: 0 kg NPK ha?1), recommended practice (20: 50: 30 kg NPK ha?1)], and two Fe treatments (non‐sprayed control and foliar FeSO4 sprays) for their effect on Fe‐chlorosis and haulm and pod yields. These treatments were tested in a strip‐split plot design with four replicates. Results revealed that TMV‐2 and ICGS‐11 were susceptible to Fe‐chlorosis and produced significantly smaller haulm and pod yield, whereas, ICGV‐8603 1 was tolerant to Fe‐chlorosis. Farmer's fertilizer practice had the highest incidence of Fe‐chlorosis. Extractable Fe and chlorophyll content in the fresh leaves were the best indices of Fe‐status and were significantly (P<0.01) correlated with visual chlorosis ratings. Foliar application of FeSO4 (0.5 w/ v) was effective in correcting Fe‐chlorosis and increased pod yield by about 30 to 40% in susceptible genotypes. These results suggests that use of tolerant genotypes such as ICGV‐86031 or foliar application of FeSO4 in susceptible genotypes such as TMV‐2 and ICGS‐11 in combination with recommended fertilizer levels is an effective management package for alleviating Fe‐chlorosis in groundnut.  相似文献   

5.
Iron (Fe) chlorosis reduces the concentration of photosynthetic pigments, photosynthates, and crop yield. The effect of Fe chlorosis on leaf composition and cell structure was evaluated in Mexican lime (Citrus aurantifolia) with different degrees of Fe chlorosis. Iron chlorosis significantly reduced concentrations of chlorophylls a, b, and a + b, and caused thickening of leaves, due to the increase in palisade and spongy parenchyma cells. The chloroplasts of the chlorotic and albino leaves showed a disorganized ultrastructure; they had an elongated shape with disarrayed thylakoids, underdeveloped grana, scarce starch granules, and hole-like folds in the thylakoid membranes. The accumulation of calcium oxalate crystals in the upper and lower sides of the epidermis, crystal length, and total crystal content increased with Fe chlorosis severity. The green leaves, in contrast, had chloroplasts with typical ultrastructure. The degree of Fe chlorosis in the leaves significantly affected the concentrations of potassium (K); Fe, manganese (Mn), Fe2+, and the phosphorus (P)/Fe and K/calcium (Ca) ratios.  相似文献   

6.
The timing and rate of application of iron (Fe) chelates (seques‐trene 138 Fe) to correct Fe chlorosis of peanut grown on calcareous soils was studied for three seasons (1985–87) in seven experiments. It was found that the biological yield of peanut increased up to the highest rate of chelate used (8 g/m2). However, under the existing prices of the chelate and peanuts, application is financially untenable above 4 g/m2. The time of chelate application should not be at sowing, but after the crop becomes chlorotic. Even though the yield differences between early application at branching, 25 to 30 days after emergence, and an application at anthesis, 45 to 50 days after emergence, was small, the trend in yield and size of nuts favored the earlier application. Therefore, it is recommended that the application of the Fe chelate be at the early stages of plant development, particularly in cases of severe chlorosis.  相似文献   

7.
Tumorous crown gall tissue in sunflower (Helianthus annus L.) initiates a mechanism for making Fe available to itself as evidenced by its ability to reduce Fe3+ to Fe2+. The objective of this study was to determine if a limited Fe supply to the plant might affect the growth, nutrition and reduction of Fe3+ to Fe2+ by the tumorous crown gall. Healthy green 14‐day‐old sunflower plants (cv mammoth Russian) were either stem‐inoculated with Agrobacterium tumefaciens to induce tumorous crown gall tissue development or were left uninoculated for comparison. The plants were grown in a modified Hoagland nutrient solution with treatments containing 0.0, 0.15, 0.6 and 2.0 mg Fe L‐1. The 0 mg Fe L‐1 treatment induced maximum Fe chlorosis, and consequently there was a release of hydrogen ions and of a yellow pigment by the roots, but there was no measureable release of ‘reductants’ by the roots. Iron‐deficiency stress (0 mg Fe L‐1) also resulted in reduced tumorous crown gall growth, less reduction of Fe3+ to Fe2+, and lower levels of Fe in the tumorous tissue compared to tumorous tissues adequately supplied with Fe. The tumorous crown gall tissue on the stem reduced much more Fe3+ to Fe2+ than the nontumorous stem tissue regardless of Fe level in the treatment. Tumor tissue contained more Fe, Cu and P than the nontumorous stem tissues which may indicate a modified metabolism in this tissue. An abundant supply of Fe seems to enhance the development and growth of the tumorous crown gall tissue and a deficient supply of Fe retards its growth.  相似文献   

8.
This study addressed some complementary aspects related to plant Fe nutrition. A field and a greenhouse experiment were conducted to monitor changes in chlorophyll, Fe3+, Fe2+, Ca2+ and K+ along with the progressive evolution of lime‐induced chlorosis, and following soil (Fe‐EDDHA, Fe‐EDTA, Fe‐DTPA, DTPA) and foliar (Fe‐EDDHA, FeSO4, “Fe‐Metalosate") treatments, in a chlorosis‐susceptible ornamental plant, Hydrangea macrophylla, over a year's growing period. Though soil Fe‐EDDHA was the most effective compound in alleviating chlorosis symptoms, it became less so with time and was only partly effective as a foliar spray. Leaf analysis showed that as chlorosis intensified and chlorophyll content decreased, phenanthroline ‐ Fe (Fe2+) decreased with corresponding increases in total iron (Fe3+) and K+ concentrations. The reliability of these chlorosis‐indicators was confirmed as the reverse changes occurred upon chlorosis plant recovery.  相似文献   

9.
In growth chambers, cotton (Gossypium hirsutum L. genetic selection ‘M8') was grown in a synthetic growth medium under four light regimes: low pressure sodium (LPS), LPS + Incandescent (Inc), cool white fluorescent (CWF) and CWF + Inc lamps at 22 C under LPS lamps. Less chlorosis developed at 26 C than at 22 C and less under LPS + Inc than under LPS lamps. All plants were green under CWF and CWF + Inc light. Green and chlorotic plant tissue contained about the same concentrations of Fe. The proposed hypothesis was that chlorotic tissue’ contained less Fe2+ than green tissue. Chlorotic leaves treated with FeSO4 turned a green color. Enough CWF + Inc light passed through an intact leaf to reduce Fe3+ to Fe2+ in vitro. Also in vitro, Fe3+ was reduced by CWF, by Inc, but not by LPS light. The amount of Fe3+ reduced during an illumination period was directly proportional to the quantity of light used. In vitro, citrate and malate enhanced Fe3+ reduction, whereas phosphate, pyrophosphate, OH‐, Cu2+, Ni2+, Mn2+, Zn2+, and F‐ all inhibited Fe3+ reduction by light. Orthophosphate was about 8 times as effective as organic P in decreasing Fe3+ reduction. Citrate largely alleviated the inhibitory effects of Pi and pH (up to pH 6). The data also provide a possible explanation of a role for many of the elements known to induce or aggravate Fe chlorosis (inhibit Fe3+ reduction). Quantity and quality of light apparently play key roles in plant growth as related to reduction of Fe3+ to Fe2+ in plant tops.  相似文献   

10.
The popular foliage houseplant, Tolmiea Menziesii (piggyback plant), grown in an all NO3‐N, half‐strength Hoagland Solution No. 1 without Fe or with 0.5 g/liter Fe2O3 became severely Fe chlorotic and caused the nutrient solution pH to rise from 5.1 initially to above 7. Plants supplied 90 μM Fe‐EDTA also raised solution pH but did not become chlorotic. When Fe chlorotic plants were transferred to a solution with 0.5 gAiter Fe2O3, modified to contain 25 to 100% of the N as NH4, the solution pH dropped to between 4.3 and 3.1, and the chlorotic plants regreened. However, if the pH of the modified solution was buffered above 7 with 1 g/liter CaCO3, no regreening occurred. Solution pH also dropped if the solution lacked N, and there was a temporary regreening of Fe chlorotic plants before N deficiency chlorosis appeared. These solution culture results indicate that Tolmiea should be classified as an Fe inefficient plant.  相似文献   

11.
Onion (Allium sativum) plants grown without iron (Fe) in sterile nutrient solutions readily developed chlorosis symptoms. Iron deficiency in the sterile‐grown plants stimulated the rates of root extracellular reduction of Fe3+, copper (Cu2+), manganese (Mn4+), and other artificial electron acceptors. While rapid reduction occurred with the synthetic chelate Fe3+HEDTA, no short‐term reduction occurred with the fungal siderophore Fe3+ferrioxamine B (FeFOB). In addition to the increased rate of extracellular electron transfer at the root surfaces, the Fe‐deficient plants showed greater rates of Fe uptake and translocation than the onion plants grown with Fe. The rates of uptake and translocation of Fe were sharply higher for the Fe‐deficient plants supplied with FeHEDTA than for similar plants supplied with FeFOB. Inhibition by BPDS of the Fe uptake by the Fe‐deficient onion plants further supported the importance of Fe3+ chelate reduction for the uptake of Fe into the roots. Rates of Fe uptake and translocation by Fe‐deficient onion plants supplied with 55FeFOB were identical to the rates of uptake of ferrated [14C]‐FOD; a result that gives evidence of the uptake and translocation of the intact ferrated siderophore, presumably by a mechanism not involving prior extracellular Fe3+ reduction. Differences in the rates of transport of other micronutrients into the roots of the Fe‐deficient onion plants were evident by the significantly higher Zn and Mn levels in the shoots of the Fe‐deficient onion.  相似文献   

12.
In comparison studies (11, 12), monocotyledonous corn (Zea mays L.) and oats (Avena byzantina C. Koch) did not respond to Fe stress as effectively nor to the same degree as the dicotyledonous soybeans (Glycine max (L.) Merr.) or tomatoes (Lycopersicon esculentum Mill.). Both the Fe‐inefficient and Fe‐efficient corn and oats developed Fe chlorosis; the Fe‐efficient dicotyledonous plants were green. In the present study, the method of inducing Fe stress was changed to make it less severe. Instead of using only NO3‐N and no Fe to induce Fe stress (11, 12), both NH4‐N and NO3‐N were used along with varied concentrations of Fe. Iron stress was induced with BPDS (4,7‐diphenyl‐l, 10‐phenan‐throline disulfonic acid) and phosphate; both competed with the plant for Fe. Phosphate also inhibits reduction of Fe3+ to Fe2+ (12). This method of inducing Fe stress in the plants was less severe than using only NO3‐N and no Fe in the nutrient solutions and we were able to measure a difference in Fe‐stress response for all four plant species (Fe‐inefficient and Fe‐efficient). At the lower Fe treatments, the roots of Fe‐efficient plants usually reduced more Fe3+ to Fe2+ than did the roots of Fe‐inefficient plants. The ‘inefficient’ ys1 corn and TAM 0–312 oat roots did not compete with BPDS or phosphate for Fe as effectively as did the ‘efficient’ WF9 corn and Coker 227 oat roots. The same type mechanism for solubilization, absorption, and transport of Fe seems to function in both monocotyledenous and dicotyledenous plants but it is more effective and more readily detected in the dicot than in the monocot plants. The reactions involved in reduction of Fe3+ to Fe2+ seemed to be confined inside or at the root surface for the inefficient genotypes; the efficient genotypes alter the ambient medium to a greater degree.  相似文献   

13.
Previous pot cropping and laboratory incubation experiments were consistent with field observations showing that temporary flooding before cropping can increase the availability of soil Fe to plants. To study the effect of temporary flooding on changes in soil Fe phytoavailability we used 24 highly calcareous, Fe chlorosis–inducing soils to carry out a pot experiment where peanut and chickpea were successively grown after flooding for 30 d. At the end of the cropping experiment, the preflooded soil samples exhibited higher concentrations of acid oxalate‐, citrate/ascorbate‐ and diethylenetriaminepentacetic acid (DTPA)–extractable Fe (Feox, Feca, and FeDTPA, respectively) than the control (nonflooded) samples. Also, Feox and Feca exhibited no change by effect of reflooding of the cropped soils or three wetting–drying cycles in freeze‐dried slurries of soils previously incubated anaerobically for several weeks. Leaf chlorophyll concentration (LCC) in both peanut and chickpea was greatly increased by preflooding. The best predictor for LCC was Feox, followed by Feca and FeDTPA. The LCC–soil Fe relationships found suggest that the Fe species extracted by oxalate and citrate/ascorbate from preflooded soils were more phytoavailable than those extracted from control soils. However, the increased phytoavailability of extractable Fe forms was seemingly limited to the first crop (peanut). Flooding dramatically increased FeDTPA; however, high FeDTPA levels did not result in high LCC values, particularly in the second crop. Therefore, this test is a poor predictor of the severity of Fe chlorosis in preflooded soils.  相似文献   

14.
Radioactively labeled iron (59Fe) was used to study differential uptake in sorghum plants in the recovery stage of chlorosis. Radio-labeled 59Fe was supplied through root feeding in nutrient solution experiment (48 hrs, pH 6.2) to non-chlorotic and chlorotic plants. Chlorotic plants were further treated with foliar spray [ferrous sulfate (FeSO4), FeSO4 + thiourea (TU), FeSO4 + citric acid (CA), FeSO4 + thioglycollic acid (TGA)] to study the uptake of radio-labeled 59Fe through root feeding during recovery process of chlorosis. Under iron deficiency, the differential uptake of 59Fe was markedly increased in leaves and stem of chlorotic control (-Fe) sorghum plants as compared to non-chlorotic control (+Fe) and foliar sprayed (FeSO4, FeSO4 + TU, FeSO4 + CA, and FeSO4 + TGA) plants. The lowest uptake of 59Fe was observed in younger leaves (24.33 nmol, g?1 fresh weight h?1) and stem (1.98 nmol, g?1 fresh weight h?1) of non-chlorotic control followed by foliar sprayed plants in comparison to chlorotic control, respectively. Similarly less 59Fe uptake was observed in the older leaves of FeSO4 + CA sprayed (21.70 nmol, g?1 fresh weight h?1) plants in comparison to chlorotic control (35.60 nmol, g?1 fresh weight h?1). The highest differential 59Fe uptake through nutrient medium was in the roots of plants, which were foliar sprayed with FeSO4 along with TU. The role of iron alone and along with citric acid and thiol compounds is discussed in recovery of chlorosis.  相似文献   

15.
Bush beans (Phaseolus vulgaris L. cv Contender) were grown on perlite with nutrient solution and 0, 1, 2.5 and 5 ppm levels of Na2CrO4 Significant decrease of top growth and chlorosis in trifoliated leaves were observed for 2.5 and 5 ppm Cr, with Cr concentrations (μg/g) in tops:≥ 12.1, in roots:≥ 509.9. Cr decreased K, Na, Mg and Fe concentrations, and increased P and Mn concentrations in roots. In tops decreased N, K, Na and Fe concentrations and increased Mn and Ca concentrations were observed, Translocation of P, Zn, Cu and Fe was inhibited; Ca and Mn translocation was generally enhanced. P/Fe ratio was increased up to 60% in chlorotic plants, indicating a shift from Fe2+ to Fe3+.  相似文献   

16.
Results of a field experiment designed to assess the effects of phosphate carriers, iron (Fe), and indoleacetic acid (IAA) on the Fe nutrition of peanut grown on a calcareous soil showed that single superphosphate (SSP) was more effective than diammonium phosphate (DAP) in improving Fe nutrition and chlorophyll synthesis. Increased phosphorus (P) and Fe contents of chlorotic leaves showing symptoms of Fe deficiency suggested that Fe, despite absorption and uptake, was subjected to inactivation, and that the Fe content per se was not the cause of the observed chlorosis. Better amelioration of chlorosis with the SSP treatment as compared with DAP indicated a role of sulphur (S) in preventing inactivation of Fe, possibly caused by excessive P accumulation. A foliar spray of Fe‐EDDHA corrected the chlorosis, but a ferric citrate foliar treatment did not. This further suggested that the mobility of Fe was impaired in chlorotic plants. An IAA foliar spray only also tended to improve Fe nutrition. Significant increase in peanut productivity was observed following improvement in Fe nutrition both with soil and foliar treatments.  相似文献   

17.
The jute (Corchorus capsularis L.) cv. 3RC‐212 which is Fe‐efficient, was subjected to Fe‐deficiency stress, and the nutrient medium was examined for chemicals, when the plants became chlorotic and the pH was lowered to about 4. While phenolic acids could not be detected, DBP (dibutyl phthalate) was identified in the extract by means of TLC and HPLC. The effect of DBP and caffeic acid was studied in JRC‐212 and DBP was found to cause recovery of the plants from chlorosis in 5 days. The chemicals, PA (phthalic acid), a derivative of DBP (50 mg/1) were supplied to chlorotic plants of JRO‐632, an Fe‐inefficient jute cultivar, and both the chemicals were effective in chlorosis recovery. PA application caused more rapid greening than DBP.

Jute is the second crop species in which DBP is identified in the root exudate. The detection of DBP was first recorded in sorghum CSH‐7.  相似文献   


18.
To assess the role of salicylic acid (SA) supplied with 5 approaches in alleviating chlorosis induced by iron (Fe) deficiency in peanut plants growing on calcareous soil, SA was supplied as soil incorporation, making slow-release particles, seed soaking, irrigation and foliar application. SA application, particularly, SA supplied by slow release particles, dramatically increased growth parameters, yield and quality of peanut, and increased Fe concentration in peanut grain. Meanwhile, SA application increased the H+-ATPase activity, reduced pH of soil, increased Fe3+-Chelate Reductase (FCR) activity in roots, and increased Fe concentration in roots. Furthermore, SA increased active Fe content and increased chlorophyll content. In addition, SA improved enzymes activities containing superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and protected Fe deficiency induced oxidative stress. Therefore, SA has a good effect on alleviating chlorosis induced by Fe deficiency on calcareous soil. However, in the 5 SA supplied approaches, foliar application and making slow release particles were more effective.  相似文献   

19.
Abstract

Genotypic evaluation is critical to development of soybean [Glycine max (L.) Merr.] cultivars with genetic resistance to Fe‐deficiency chlorosis. Root Fe3+ reducing activity is correlated with genotypic resistance to Fe chlorosis measured in field nurseries, and thus may be a reliable method for identifying chlorosis‐resistat genotypes. However, to develop methods useful for large‐scale screening, several modifications of the previously published procedure for measuring root Fe3+ reducing activity were investigated. Several hydroponic experiments were conducted to test proposed modifications. It was determined that: (a) different genotypes may be grown together in the same nutrient solution without affecting Fe3+ reduction, (b) genotype separation is maximized by growth in CaCO3 buffered solution (37.5 mg L?1), (c) a labor‐intensive elongation step can be eliminated, and (d) denotype evaluation can be accomplished without introducing Fe into the hydroponic solutions. These refinements to the procedure should allow its adaptation and use in soybean breeding programs.  相似文献   

20.
Sodium carbonate added to nutrient solution in sand culture depressed the growth of tomatoes both by the influence of high pH and HCO3? causing chlorosis and by the effect of Na+. Foliar sprays of iron and manganese removed chlorosis and increased growth but did not remove the effect of Na. In a sandy soil Na2CO3 did not cause chlorosis but Na+ depressed yield. Chelated Fe and Mn in the soil solutions (up to 7.3 × 10?4 M Fe and 2.6 × 10?5 M Mn at pH 9.0) were sufficient to supply the crop needs as shown by a second sand culture experiment where plants were fed with nutrient solution plus extracted soil solution.  相似文献   

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