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对单叶省藤、短叶省藤组培快繁成苗芽的选择研究试验结果表明:生长健壮、形态正常的不同高度未展叶单芽组,在成苗培养过程中,高生长、生根率、生根条数和生根长度存在差异。高度在2.1~4.0cm的未展叶单芽为伸长培养和生根诱导培养的最佳高度组,生根诱导培养3个月后,芽苗生长可达长到组培苗移植规格(苗高、根长〉4.0cm)。研究试验结果还显示,2种省藤组培成苗培养过程,伸长及生根诱导培养可浓缩为一步,直接从增殖培养材料中选择2.1~4.0cm的单芽进入生根诱导培养,一步成苗。 相似文献
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不同碳源对草莓脱毒苗组培快繁的影响 总被引:7,自引:0,他引:7
实验在筛选出草莓脱毒快繁培养基的基础上,对影响草莓组培繁殖系数的碳源因素进行了探讨研究。结果显示:蔗糖和市售白砂糖为草莓组培快繁的最佳碳源,恒温培养26天,繁殖系数均能达到15以上,从经济角度考虑,可以用白砂糖取代蔗糖;用白砂糖作碳源,当浓度为3%时,草莓脱毒组培苗的繁殖系数最高,长势最好。 相似文献
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以早实薄皮核桃‘绿岭’成熟种胚诱导出的无叶腋芽为外植体,MS为基本培养基,通过调节激素种类和浓度配比,初步研究了‘绿岭’核桃种胚无叶腋芽快繁体系。比较试验结果表明:最佳种胚萌发诱导培养基为MS+IBA 1.0mg/L+6-BA 1.0mg/L+蔗糖30g/L+琼脂6g/L;种胚无叶腋芽伸长培养基为MS+6-BA 2.0mg/L+IAA 1.0mg/L+GA31.5mg/L+蔗糖30g/L+琼脂6g/L。生根采用两步生根法:离叶柄基部2 mm切的茎段先在DKW+IBA 8.0mg/L+蔗糖30g/L+琼脂6.0g/L暗培养20d,然后转入DKW+蔗糖30g/L+琼脂6g/L培养基中进行光照培养,生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%。 相似文献
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探讨了大量元素含量对我国主要栽培藤种黄藤、短叶省藤和单叶省藤组培增殖的影响,确定了各大量元素的适宜范围.以优化的配方进行多次继代培养后,抽取离体芽和培养基进行大量元素含量测定,评价配方的好坏和确定多次继代使用是否需要变换配方 相似文献
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研究了橡皮树的组织培养流程及技术要点,进行了最佳培养基配方的筛选,通过数据汇总分析总结出了切实可行的最佳培养基和快繁技术。 相似文献
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In the western United States, many native riparian forests are declining downstream from dams and diversions, primarily due to a lack of successful regeneration of native species. Conceptual models have been described that incorporate stream flow characteristics that promote successful regeneration of western riparian Populus seedlings. However, these models need to be calibrated for additional species and specific stream characteristics. In this study, we examined the growth and survival of two dominant Sonoran desert riparian tree species, a native Salix gooddingii, and an exotic, Tamarix chinensis, to simulated water table declines at rates of 0, 1, 2, and 4 cm/day. Salix seedlings performed best in the 0 cm/day treatment in which the soil was saturated and showed decreasing survival and growth with increasing rates of water table decline. Root-to-shoot ratios were high for Salix, but root elongation rates were low with an emphasis on lateral root development. This root growth pattern may be an adaptation to habitat where Salix typically grows, near the stream channel where the water table is shallow but the likelihood of flood scour is high. Lateral root development may help to protect Salix seedlings from late season flood scour. Tamarix seedlings showed consistently high survival across all treatments and had the greatest growth in the 0 and 1 cm/day treatments. Tamarix seedlings had greater root elongation rates in response to water table decline relative to the control, with the greatest elongation in the 1 cm/day treatment. Greater root elongation rates and higher drought tolerance allowed Tamarix seedlings to persist in dry soils where Salix seedlings died. Differences in growth and survival of these two species in response to water table decline will need to be incorporated into management practices to promote successful native riparian tree regeneration. 相似文献
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西双版纳2008年成功引进橡胶树籽苗芽接技术,并已向生产推广。籽苗芽接用播种后约2周的种子籽苗芽接,苗圃培育7~10个月,可育成2~4篷叶、茎干直径0.4cm以上的成苗;苗木定植成活率高,雨季定植可达99.85%;植后恢复生长快,植后6月龄7篷植株可达19.73%,降低了生产成本和芽接劳动强度,提高了苗圃单位面积生产率。 相似文献
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The aim of this study was to describe the dynamics of seedling establishment and development in spruce-dominated uneven-aged boreal forests. The study was based on empirical data from 15 stands with permanent plots, which had been intensively monitored for 10 years in southern Finland. All trees (height > 1.3 m) were measured every fifth year. Regeneration was measured on 64 permanent sample plots (4 m2) in each stand. The establishment of first-year seedlings was analysed on a yearly basis. The survival and development of older Norway spruce (Picea abies (L.) H. Karst.) seedlings were analysed based on observations made every five years. The establishment of spruce seedlings was closely correlated with the abundance of seed crops. Seedling cohorts originating from abundant seed crops were clearly detectable in the development of seedling height distributions over time. It took about 15 years for spruce seedlings to reach a height level of 15-30 cm. Local basal area had hardly any effect on the emergence or survival of small spruce seedlings, while the number of higher spruce seedlings decreased with increasing local basal area. 相似文献
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福建省木荷苗木标准的研究 总被引:1,自引:0,他引:1
对近2a福建省各地培育的1年生木荷播种苗进行调查,应用分层抽样技术和逐步聚类分析,对苗木质量进行分级,并结合生产实际探讨了福建省木荷苗木标准:Ⅰ级苗地径0.7cm,苗高65cm,Ⅱ级苗地径0.5cm,苗高45cm,合格苗率80%,密度70株/m2,各指标值大于现行国家标准,能较好地反映实际生产水平,为育苗生产和制定地方标准提供参考 相似文献
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普拉索芦荟组织培养技术研究 总被引:1,自引:1,他引:1
取普拉索芦菩嫩茎作外植体,培养于附加不同种类和激素浓度的MS或改良的MS培养基上,在附加 6-BA1.5 mg/L时,丛生芽诱导效果最佳,萌发数量最多;在附加 6-BA1.0mg/L+NAA0.3mg/L时,丛生芽生长最好,芽长且健壮,;在附加I AA0.2 mg/L时,生根效果最佳;当苗高 3 cm以上,根长达2 cm以上时,开盖 3 d移入温室(10~16℃),炼苗 3 d后,移栽于蛙石;河沙;腐质土为0.5:1:1.5的混合基质的土壤中,成活率达91.3%。 相似文献
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通过对山皂角采取营养钵和苗床两种方法进行育苗试验,结果表明:山皂角采用营养钵育苗,无论是树高还是地径生长量,均较对照苗床育苗高,出苗率达到96.4%,平均苗高达到59.3cm,平均地径达到0.41cm。山皂角的营养钵育苗有利于生产高品质的苗木,提高造林成活率。 相似文献
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云南高氏甜龙竹苗木质量分级及造林效果 总被引:1,自引:1,他引:0
为保障高氏甜龙竹(商品名)苗木质量,提高造林效果,在昆明市嵩明县、富民县,保山市昌宁县,玉溪市元江县开展了播种育苗和扦插育苗试验,以苗高、地径为分级指标研究了高氏甜龙竹1年生苗的分级标准。结果显示,不同地区苗木的分级标准具有一定差异,其中富民县和元江县对Ⅰ级苗的要求较高,富民县Ⅰ级播种苗要求苗高≥8.31 m、地径≥4.63 cm,元江县Ⅰ级扦插苗要求苗高≥6.55 m、地径≥3.67 cm。高氏甜龙竹不同育苗方式,其苗木生长状况存在差异,表现为播种苗的各项生长指标均好于扦插苗,特别是根系的生长状况和每丛株数,播种苗的平均根系数量、根系总质量、每丛株数分别是扦插苗的7.07倍、7.13倍和6.69倍,表明播种苗以后的生长潜力更大。不同等级的高氏甜龙竹苗木的造林效果,无论是播种苗还是扦插苗,Ⅰ级苗的造林成活率均达到100%,Ⅱ级苗的造林成活率也可达到95%以上,Ⅲ级苗的造林成活率均低于82%。 相似文献