共查询到20条相似文献，搜索用时 203 毫秒
1.
对北京市周边6省份怀疑感染鹦鹉热嗜性衣原体的鸡鸭血清样品374份、病料81份，分别使用IHA诊断试剂盒、ELISA试剂盒以及抗酸染色试剂和荧光抗体诊断试剂进行了检查，以评价北京市及其周边地区家禽鹦鹉热嗜性衣原体的流行性。结果，上述4种试剂检测出的阳性率依次为24．9％、77．9%、18．5%和38．2％；北京市10份SPF鸡血清的抗体全部为阳性；患病肉鸡、肉鸭气囊样品，蛋鸡输卵管样品的检出率较高。表明，北京市及其周边地区家禽已经感染了鹦鹉热嗜性衣原体，酶联免疫吸附法和荧光抗体染色法能分别提高抗体和抗原的检出率。 相似文献
2.
《中国家禽》2016,(7)
鸡传染性贫血病病毒(CIAV)是禽弱毒疫苗中常见外源病毒之一,选择未被CIAV污染的SPF鸡胚是生产合格疫苗的关键环节,疫苗生产企业通常通过检测SPF鸡血清中CIAV的抗体来评估CIAV感染状况,本研究以取样更为方便的卵黄抗体检测来评估SPF鸡群中CIAV感染状态。将40只SPF鸡于19周龄时人工接种CIAV,同时30只SPF鸡饲养于另一隔离环境内作为空白对照。23周龄时每只鸡分别采集种蛋和血清并一一对应,然后将卵黄分别进行1∶10、1∶20、1∶30稀释后与血清同时检测CIAV抗体,结果发现卵黄抗体1∶20倍稀释后判定结果与血清抗体吻合率最高。对70只SPF鸡从25~34周龄进行连续10周观察,结果显示卵黄抗体检测结果与血清抗体检测结果吻合率达91.57%。建议疫苗生产企业可通过抽检卵黄抗体来判断SPF鸡群的CIAV洁净度。 相似文献
3.
拟研究以卵黄抗体替代血清抗体检测判定SPF鸡群禽白血病病毒(ALV)感染状态的可行性。在人工接种ALV-A/B后的40只SPF鸡刚开产时,从21只抗体阳性鸡及19只抗体阴性鸡分别采集血清和卵黄并一一对应,比较卵黄不同稀释度与血清中ALV-Ab抗体的阴阳性吻合率及ELISA检测S/P值相关系数。另外36只同批次不攻毒的SPF鸡单独饲养作为对照,76只鸡在25~34周龄期间,每隔3周分别采集1次血清,每周采集1次种蛋,共采集了304份血清样品及836份卵黄样品。所有血清和卵黄抗体用IDEXX的ALV-Ab抗体ELISA检测试剂盒检测。血清按试剂盒规定的1∶500稀释,卵黄按预试验结果选定稀释度稀释。同一只鸡同一时段采集的血清和卵黄样品,严格在同一次ELISA试验中检测。结果表明,相对于血清抗体,将卵黄做1∶300稀释时,假阳性和假阴性最少,确定1∶300为卵黄最适稀释度。在25~34周龄,836份卵黄抗体的检测判定结果与304份血清抗体检测结果吻合率达94.7%,即这些血清抗体阳性鸡同时期采集的卵黄抗体也全部阳性,血清阴性鸡的卵黄抗体也均为阴性。结果提示疫苗生产企业可通过检测SPF种蛋的卵黄抗体来判断SPF鸡场对ALV的洁净度。 相似文献
4.
5.
为研究以卵黄抗体替代血清抗体判定SPF鸡群J亚群禽白血病病毒(ALV-J)感染状态的可行性,人工接种ALV-J的SPF鸡23周龄时,分别从25只抗体阳性鸡及22只抗体阴性鸡采集血清和卵黄,比较不同稀释度的卵黄与血清中ALV-J抗体的阴阳性吻合率及ELISA检测S/P值相关系数。结果表明,相对于血清抗体,将卵黄1∶400稀释,假阳性和假阴性最少,确定1∶400为卵黄最适稀释度。对40只攻毒SPF鸡和36只同批次单独饲养的空白SPF鸡在25~34周龄,每隔3周采集一次血清,每周采集一次种蛋,共采集304份血清样品和760份卵黄样品。血清按1∶500稀释,卵黄按1∶400稀释,所有血清和卵黄抗体用美国IDEXX公司ALV-J抗体ELISA检测试剂盒检测,同一只鸡同一时段采集的血清和卵黄样品严格在同一次ELISA中检测。结果显示,在25~34周龄,卵黄抗体检测判定结果与血清整体吻合率为82.5%~95%。上述结果表明用卵黄抗体替代血清样品检测来监控SPF鸡群对ALV-J的感染状态是可行的,疫苗生产企业可通过抽检SPF鸡场提供SPF种蛋的卵黄抗体水平来判断SPF鸡场的洁净度。 相似文献
6.
本试验将新城疫（ＮＤ）疫苗与Ｂ１１００１株鹦鹉热衣原体混合，用ＮＤ阳性血清中和，每个接种剂量最终含ＮＤ疫苗１０羽份，衣原体浓度分别为１０~（－９）、１０~（－２）、１０~（－３）、１０~（－４），分组接种ＳＰＦ鸡胚进行测定，检测了９株１０~（－４）稀释的衣原体。并以Ｂ１１００１株衣原体试验性污染５批ＮＤ疫苗作检测。试验中取卵黄囊膜涂片，用中科院动物所提供的衣原体萤光抗体试剂盒进行检测，并辅以姬姆萨染色法检查，结果表明用荧光抗体染色可以检查到疫苗中衣原体或包涵体的存在，与姬姆萨染色法基本一致。 相似文献
7.
间接血凝试验检测衣原体抗体的研究 总被引：1，自引：0，他引：1
用标准株沙眼衣原体 T_(55)株和鹦鹉热衣原体 B_(11001)株感染的鸡胚卵黄膜乙醚浸提抗原致敏的公绵羊红细胞和两个标准株的高免血清分别与湖北分离的4株鹦鹅热衣原体的实验感染动物的抗血清和乙醚抗原致敏的绵羊红细胞进行间接血凝试验(IHA)双交叉反应,并与补体结合试验(CF)和间接补体结合试验(ICF)进行比较。结果表明,IHA 在检测衣原体抗体中能发生特异性的双交叉反应,而且重复性良好,比 CF 敏感8～16倍,比 ICF 敏感64～128倍。IHA 的效价高达1:4096～8192。用 IHA 和 CF 两法同时检测自然发病猪群的血清样品212份,阳性率分别为44.8%和44.3%,二者的符合率为98.9%。 相似文献
8.
本试验对北京郊区5个规模化猪场断奶仔猪、育成猪、育肥猪和怀孕母猪采集血清共507份,同时采集密切接触饲养人员、仔猪、育肥猪的喉头拭子、母猪阴道拭子及公猪的精液共183份。分别使用间接血凝诊断试剂盒、法国ELISA试剂盒检测抗体;利用直接荧光染色法检测抗原,包括166份猪喉头拭子、阴道拭子和精液以及17份饲养人员喉头拭子。结果发现间接血凝诊断试剂盒、ELISA试剂盒抗体阳性率分别为4.14%和2.17%;抗原平均阳性率为14.8%,其中精液阳性率达到37.5%,母猪阴道拭子阳性率为27.5%,饲养人员阳性率为23.5%。本试验证实北京郊区猪嗜流产衣原体在种公猪、母猪群和饲养员呈高流行趋势。同时研究结果显示,5个被调查的规模化猪场嗜流产衣原体抗体阳性率显著低于有报道的其他省市,这与间接血凝诊断试剂盒检测结果高于ELISA试剂有关。针对发现的问题,必须采取有效措施控制种公猪精液污染衣原体现象,阻断向母猪和饲养人员传播,以降低人兽共患病发生的风险。 相似文献
9.
《中兽医学杂志》2015,(9)
目的:本研究对某肉鸡养殖场发生疑似鹦鹉热衣原体病的肉鸡群进行实验室诊断。方法:采集病鸡血清进行间接血凝试验做出初诊,将采集的病料组织接种于SPF鸡胚进行病原分离,通过特异性免疫荧光染色、姬姆萨染色观察包涵体进行鉴定。结果:血清学试验结果显示该肉鸡群感染衣原体发病率为25~30%,死亡率约为10%,病株在细菌培养基上不生长,SPF鸡胚盲传三代后4~6d鸡胚死亡,特异性免疫荧光染色,姬姆萨染色观察包涵体。结论:本研究通过血清学调查,细菌培养、病料接种SPF鸡胚进行病原分离,通过特异性免疫荧光染色,姬姆萨染色观察包涵体,初步判定该肉鸡场发病是由鹦鹉热衣原体感染所引起。 相似文献
10.
为了解山东地区家禽鹦鹉热衣原体(Cps)感染现状,本研究采用间接血凝法(IHA)对2013年~2014年采集自山东潍坊、淄博、济南、临沂、烟台等地区的1 020份鸡、鸭血清样品进行Cps抗体的检测,并对检测数据进行了统计分析;结果显示:IHA测得总阳性率为29.51%(301/1 020);鸡阳性率为25.26%(197/780);鸭阳性率为43.33%(104/240);各地区间阳性率存在一定差异。本调查结果表明,家禽Cps感染在山东地区具有较高的感染率。 相似文献
11.
This study, for the first time in Turkey, investigated the existence of Chlamydophila psittaci and determined the prevalence of its disease, chlamydiosis, in pet birds. Polymerase chain reaction (PCR) was compared with other testing methods that have been typically used in the diagnosis of C. psittaci. Fecal specimens (n =96) of avian origin were tested by PCR and two identification methods, modified Gimenez staining (mGS) and direct fluorescein-conjugated monoclonal antibody staining (FA). The identification methods were implemented by staining the yolk sacs of embryonated chicken eggs inoculated at 6 days of age and harvested between 3 and 10 days after inoculation. Fecal specimens from pet birds were randomly collected from pet shops and homes. These specimens were then used to isolate C. psittaci and to detect its specific DNA. The inocula that were prepared from fecal specimens were then inoculated into yolks of 6-day-old embryonated chicken eggs. The preparations from egg yolk sacs were examined with mGS and direct FA after three blind passages. The PCR method was used to detect specific DNA in feces. In 96 fecal specimens, 33 (34.4%) were positive with PCR, 21 (21.9%) were positive with mGS, and 29 (30.2%) were positive with FA. Among 33 positive specimens with PCR, 28 specimens were positive with FA, and 20 specimens were positive with mGS. The sensitivity and specificity were 59% and 94% between FA and mGS, and 97% and 93% between FA and PCR, respectively. 相似文献
12.
The objective of this study was to isolate and identify a hypothetical Chlamydiaceae pathogen from laying hens with an oviduct cyst, and to characterize its potential causal relation with decreased egg production. Our clinical survey showed that cystic oviducts were prevalent at rates of 10% and 15.1% in breeder and commercial hen flocks, respectively. Chlamydial antigens were detected in 20 of 50 pharyngeal swabs (40%) and in 17 of 20 oviduct tissues (85%) using enzyme-linked immunosorbent assay (ELISA) antigen detection kits. The isolated pathogen was identified as Chlamydophila psittaci via complement fixation test, PCE-ELISA, and immunofluorescence assay. Avian influenza virus, Newcastle disease virus, and infectious bronchitis virus were excluded after oviduct tissues were inoculated onto the chorioallantoic membrane of embryonating eggs. The nucleotide sequence of the omp1 gene (accession no. EF202608) from the isolate was similar to that of C. psittaci avian type C (accession no. L25436). Typical cystic oviducts were observed in specific-pathogen-free hens inoculated intraperitoneally with the isolate. The high presence of chlamydial antigen is consistent with the cystic oviducts and poor egg production. We conclude that the isolated C psittaci is most likely associated with cystic oviducts in laying hens. 相似文献
13.
青藏高原地方鸡种——海东鸡与商品鸡蛋品质分析比较 总被引：3，自引：1，他引：2
作者对青藏高原地方鸡种—海东鸡与商品新罗曼鸡的蛋品质及蛋中营养成分进行测定并分析比较。结果表明,海东鸡的蛋重、蛋壳重、蛋白重及蛋白重/蛋重极显著低于新罗曼鸡(P0.01);而蛋形指数和蛋黄重/蛋重极显著高于新罗曼鸡(P0.01);蛋壳厚度和蛋壳重/蛋重海东鸡显著高于新罗曼鸡,且海东鸡的哈氏单位显著高于新罗曼鸡(P0.05);蛋黄和蛋壳强度重二者无显著差异。海东鸡蛋全蛋粗蛋白质含量比新罗曼鸡蛋低21.3%,达到极显著水平(P0.01);粗脂肪含量比新罗曼鸡高18.1%,达到显著水平(P0.05)。 相似文献
14.
The objective of this study was to isolate and identify suspected pathogens from peacocks and peacock farmers with severe pneumonia and to investigate its potential association with peacocks' pneumonia, caused by Chlamydophila psittaci infection. A clinical examination of infected peacocks identified birds with symptoms of anorexia, weight loss, yellowish droppings, airsacculitis, sinusitis, and conjunctivitis, whereas the infected farmers showed high fever and respiratory distress. Immunofluorescence tests detected chlamydial antigens in pharyngeal swabs (12 of 20) and lung tissue samples (four of five) from peacocks. One of four swabs taken from farmers was also positive by the same test. Specific anti-chlamydia immunoglobulin G was detected in 16 of 20 peacocks and four of four peacock farmers. The isolated pathogen was able to grow in specific-pathogen-free (SPF) chicken embryos and McCoy cell lines and was identified as Chlamydiae by immunofluorescence assay and PCR. Avian influenza virus, Newcastle disease virus, and infectious bronchitis virus were eliminated as potential causative agents after pharyngeal swabs inoculated onto the chorioallantoic membrane of embryonate eggs failed to recover viable virus. PCR and restriction fragment length polymorphism indicated the ompA gene from the isolate was similar to that of avian C. psittaci type B. Three-week-old SPF chickens challenged with the peacock isolate via intraperitoneal injection showed a typical pneumonia, airsacculitis, and splenitis. Subsequently, the inoculating strain was recovered from the lungs of challenged birds. This is the first report of C. psittaci infection in peacocks and peacock farmers. 相似文献
15.
Gary W. Webb PhD PAS Codi L. Burris MS Sarah E. Harmon MS Rachel H. Baker MS 《Journal of Equine Veterinary Science》2011,31(4):166-173
Three experiments were conducted to determine whether replacement of chicken egg yolk, as a component of freezing extenders, with egg yolk from other avian species would improve the post-thaw motility and percentage of intact acrosomes of stallion spermatozoa. In the first experiment, substitution of chicken egg yolk with chukar egg yolk, as a component of the lactose-ethylenediaminetetraacetic acid extender, improved (P ≤ .05) the post-thaw motility of stallion spermatozoa. These results were not replicated in (IMV Technologies, Maple Grove, MN, USA) a more expansive study comparing 2%, 4%, 6%, or 8% egg yolk combined with INRA 96 when a “slow freeze” method was used, or the same substitution at levels ranging from 13% to 22% when egg yolk was combined with lactose-ethylenediaminetetraacetic acid for diluents used for a “fast freeze” method of cryopreservation. In the third study, egg yolks from regular and high omega-3 chicken eggs as well as from turkey, chukar, and mallard duck eggs were analyzed for lipid content and fatty acid profile. The yolk from the turkey eggs was higher (1,300 mg/100 g) and that from mallard ducks was lower (560 mg/100 g) in cholesterol as compared with the two types of chicken eggs and chukar egg yolk (range, 1,046-1,094 mg/100 g). In addition, the high omega-3 eggs did test higher for fatty acids (4.51 g/100 g) than other types of eggs (range, 0.28-0.73 g/100 g). Substitution of chicken egg yolk with turkey, but not duck, egg yolk resulted in higher post-thaw total motility (P ≤ .05) for spermatozoa obtained from two of the three stallions used in the third experiment. 相似文献
16.
M M Wittenbrink X Wen N B?hmer G Amtsberg A Binder 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(6):411-420
By inoculation of embryonating chicken eggs via the yolk sac route Chlamydia psittaci was grown from 11 lungs of 45 pigs with pneumonia (24.4%). From the lungs of 55 pigs with other diseases the organism was isolated in five cases (9.1%). Chlamydiae were not detectable by cultural methods in the uterine mucosa of 87 sows, arthritic joints of 30 store pigs and in aborted fetuses. A commercial available enzyme amplified immunoassay indicated the presence of chlamydial antigen in mucosal scrapings from the uterus of two sows and in the fetal membranes as well as fetal organs in one case of porcine abortion. 相似文献
17.
不同品牌市售鲜鸡蛋贮存过程中微生物变化比较研究 总被引：2，自引：0，他引：2
鸡蛋是人类膳食的重要组成部分,其产出与贮运销售过程中均存在沙门氏菌等致病菌污染隐患,直接影响鸡蛋的消费安全。作者对北京市场德青源、留民营等4种品牌包装鲜鸡蛋及散装鸡蛋在贮存过程中细菌总数、大肠菌群数及沙门氏菌数量变化进行了测定,研究了贮存过程中各品牌鸡蛋蛋壳、蛋清及蛋黄中的细菌总数,大肠菌群数随时间的变化情况及沙门氏菌的检出情况。研究结果发现,不同品牌的鸡蛋蛋壳表面在贮存初期细菌总数介于9.315×102～1.367×104 CFU/个,且随着贮存时间的延长呈上升趋势;品牌鸡蛋贮存初期蛋内容物均未检出污染细菌,而在贮存6 d后检出污染细菌,细菌总数与大肠菌群存在随着贮存时间的延长而增加的变化。除A品牌鸡蛋外,市售鲜蛋均存在沙门氏菌污染,蛋壳表面污染率为1.67%～8.3%,内容物中污染率为1.67%～5%。其中散装鸡蛋的细菌总数,大肠菌群数及沙门氏菌检出率均高于其它几种品牌的鸡蛋。 相似文献
18.
试验旨在研究饲粮中添加不同水平凝结芽孢杆菌对蛋鸭产蛋性能、蛋品质及血清生化指标的影响。选取200日龄,产蛋率约80%的临武鸭240羽,随机分成4组,每组6个重复,每个重复10羽鸭,对照组饲喂基础饲粮,试验组在基础饲粮中分别添加100 mg/kg、150 mg/kg和200 mg/kg凝结芽孢杆菌,预试期7 d,正试期28 d。结果表明：（1）饲粮添加凝结芽孢杆菌可提高蛋鸭产蛋率、平均日产蛋量、平均蛋重和饲料转化率,但未达显著水平（P>0.05）;添加100 mg/kg和150 mg/kg凝结芽孢杆菌可显著降低产蛋鸭采食量（P<0.05）,添加200 mg/kg与各组间均无显著差异（P>0.05）;(2）饲粮添加凝结芽孢杆菌可显著降低鸭蛋蛋壳厚度（P<0.05）,当添加150 mg/kg和200 mg/kg时,差异达极显著水平（P<0.01）,凝结芽孢杆菌还可显著提高鸭蛋蛋黄颜色,蛋白高度和哈氏单位（P<0.05）;（3）芽孢杆菌对产蛋鸭血清中血糖、总蛋白、总胆固醇、甘油三脂等血清生化指标均无显著影响（P>0.05）。由此可知,饲粮中添加凝结芽孢杆菌能在一定程度上提高蛋鸭饲料转化率和产蛋性能,虽会引起蛋壳厚度降低,但不影响合格蛋率,还可提高蛋黄颜色和哈氏单位,对蛋品质有一定改善。综合考虑,蛋鸭饲粮中凝结芽孢杆菌的添加量以150 mg/kg较为适宜。 相似文献
19.
SH. Golzar Adabi M. Ahbab A. R. Fani A. Hajbabaei N. Ceylan R. G. Cooper 《Journal of animal physiology and animal nutrition》2013,97(1):27-38
Lipids are an important nutritional component of the avian egg. A review of the literature was completed to determine the fatty acid compositions in egg yolk from some avian species. Additionally, the nutritional influence of lipid and lipoprotein content on the plasma of male participants during 30‐day feeding was discussed. The ostrich eggs had the highest unsaturated fatty acid and the lowest cholesterol content in relation to other avian species. Ostrich had a higher proportion of 18:3n‐3 (p < 0.01) compared with other species. Chicken yolk numerically contained much higher levels of 22:6n‐3 than those found in turkeys, quails and geese, but the amount of 22:6n‐3 in ostrich egg was lower by comparison with other species (p < 0.01). After the storage of eggs at the room temperature, there was a notable loss of vitamin E (vitE) in the yolks of all species and this decrease was marginal (p < 0.01) in ostrich compared with other species. There were significant (p < 0.05) increases in plasma low‐density lipoprotein (LDL) level in all male subjects. Plasma high‐density lipoprotein (HDL) level decreased (p < 0.05) only in men who were fed chicken or ostrich eggs daily. Consumption of different species’ eggs had no influence on the total male plasma cholesterol and triglyceride levels. LDL‐C:HDL‐C ratio increased (p < 0.05) after goose and turkey egg consumption. Consumption of one egg/month by healthy human subjects had no effect on serum total cholesterol and triglyceride. The LDL‐C:HDL‐C ratio (which is a strong predictor of coronary heart disease risk) increased, although non‐significantly, by consuming chicken, quail and ostrich eggs. 相似文献
20.
Okuda H Ohya K Shiota Y Kato H Fukushi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(2):249-254
Chlamydophila psittaci is the causative agent of human psittacosis and avian chlamydiosis. This zoonotic pathogen is frequently transmitted from infected birds to humans. Therefore proper and rapid detection of C. psittaci in birds is important to control this disease. We developed a method for detecting C. psittaci by using SYBR Green Real-time PCR based on targeting the cysteine-rich protein gene (envB) of C. psittaci. This one step procedure was highly sensitive and rapid for detection and quantification of C. psittaci from fecal samples. This assay was also able to detect other zoonotic Chlamydophila species such as C. abortus and C. felis. The assay is well suited for use as a routine detection method in veterinary medicine. 相似文献