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1.
In a two-year experiment, three silages were prepared from herbage treated either with an inoculant at 1·25 × 105 organisms (g fresh material (FM))−1. formic acid (850 g kg−1) at 4 1 (t FM)−1, or no additive (untreated). In Experiment 1, unwilted and in Experiment 2, wilted silages were investigated and had mean dry matter (DM) and water soluble carbohydrate (WSC) concentrations at ensiling of 171 g kg−1 and 17·6 g (kg FM)−1 and 263 g kg−1 and 25·1 g (kg FM)−1, respectively. In Experiment 1, 45 and in Experiment 2, 54 individually fed cows were used to evaluate the silages in three-treatment, randomized-block design experiments. During weeks 4-12 of lactation the cows were offered silages ad libitum and during weeks 15-26 a constant amount of silage was fed. There were few major differences in chemical composition of the resulting silages. Formic acid had no effect on silage digestibility. Inoculant treatment increased digestibility when the grass had been wilted. The use of formic acid resulted in increased silage DM intake of 9% during weeks 4-12 of lactation in Experiment 1 but not in Experiment 2. The inoculant gave no increase in silage DM intake over the control in Experiment 1 but increased silage DM intake by 7% in Experiment 2. There was no significant response in milk yield to formic acid. In Experiment 2 the response in milk yield to inoculant treatment was significant both in weeks 4-12 of lactation (4%) and in weeks 15-26 of lactation (5%). It is concluded that the response in milk yield to the use of a specific inoculant appears to be mediated through increased intake of metabolizable energy (ME).  相似文献   

2.
Determination of microbial protein in perennial ryegrass silage   总被引：1，自引：0，他引：1  
The microbial matter fraction was determined in perennial ryegrass silages of different dry-matter (DM) contents, ensiled with or without Lactobacillus plantarum . 15N-Leucine and the bacterial cell wall constituent diaminopimelic acid (DAPA) were used as markers for microbial-N. Perennial ryegrass crops with DM contents of 202, 280 or 366 g kg−1 fresh weight were ensiled in laboratory-scale silos and stored for 3 to 4 months. At different times after ensiling, silages were analysed and microbial fractions were isolated. Microbial-N concentration determined with 15N-leucine reached a maximum during the first week of ensilage. It remained unchanged thereafter, except in silage with a DM content of 280 g kg−1 in which it decreased ( P  < 0·01) by 32% during storage. After 3 to 4 months ensilage, microbial-N concentration varied from ≈0·3 to ≈1·7 g kg−1 DM. A negative relationship was observed between microbial-N concentration and silage DM content. Inoculation resulted in an approximately twofold increase ( P  < 0·001) in microbial-N concentration. Microbial-N concentrations determined with DAPA were 1·14–2·07 times higher than those determined with 15N-leucine. However, 19–35% of the DAPA in silage occurred in a soluble form, indicating that this fraction of DAPA was not associated with intact bacteria.  相似文献   

3.
In three separate feeding experiments using a total of twenty-six individually-housed Ayrshire cows, three wilted silages made from Blanca white clover were offered ad libitum with either different supplements or different proportions of grass silage. The clover silages contained 680 g white clover kg−1 on a DM basis, and had a mean DM concentration of 263 g kg−1 with 231 g CP kg−1 DM and 91 g ammonia-N kg−1 N. The pH values averaged 4·16 and the DOMD concentrations 611 g kg−1. In experiment 1 the daily intake of clover silage given alone was 15.2 kg DM per cow, i.e. 30·1 g kg−1 live weight, and decreased by 0·76 kg DM kg−1 barley DM and by 0·66 kg DM kg−1barley plus soybean meal DM when these feeds were offered as supplements. Milk yield and fat concentration were higher on the supplement treatments than on the clover silage-only treatment. In experiments 2 and 3 the intakes of silage and total DM increased as the weight of clover in the diet increased from 0 to 700 g kg−1 with parallel increases in milk yield. The effects on milk composition were small and generally non-significant. Although white clover silages with excellent fermentations were made, it is concluded that the main role of white clover in a silage system will be in mixed swards with grass to reduce the input of fertilizer N and to increase the voluntary intake of silage.  相似文献   

4.
The fermentation characteristics and chemical composition of 57 first-cut and 30 second-cut samples of grass silages, made in bunker silos on commercial dairy farms in Wales in 1990, and treated with a nominal 61 t−1 of an acid salt-type additive at ensilage, is described. Typical chemical composition of grass cut for ensilage was 156 g kg−1 dry matter (DM) and 28 g kg−1 water soluble carbohydrate (WSC), with 181 g (kg DM) −1 crude protein (CP) and 232 g (kg DM) −1 modified acid detergent fibre (MADF). The effect of additive use was to produce silages with DM 230 g kg−1 pH 3·93, ammonia N 70 g kg−1 total N, with residual WSC 35 g (kg DM) −1, lactic acid 83 g (kg DM) −1, total acids 118 g (kg DM) −1 and butyric acid 0·7 g (kg DM) −1. No significant differences were found between first- and second-cut silages. Silage fermentation was restricted (i.e. lactic acid less than 60 g kg DM−1) in only 20% of the samples.
It is suggested that on commercial farms the application rate achieved may be insufficient to produce a restricted fermentation.  相似文献   

5.
Grass (220 g DM kg−1 was ensiled after adding 106 and 108 enterobacteria (90% Rahnella aquatilis , 9·9% Hafnia alvei and 0·1% Escherichia coli g−1 fresh mutter. The silages were exposed to aerobic conditions alter 125 d of storage. In all treatments H. alvei rapidly superseded the initial high number of Enterobacter agglomerans (naturally present) and R. aquatilis. The maximum number of enterobacteria was detected about I d after initiation of fermentation. After 4 d of fermentation, when concentrations of undissociated lactic and acetic acids ranged from 52 to 36 mM and 41 to 51 mM respectively, no enterobacteria were detectable. Inoculating the crop with enterobacteria restilted in a temporarily retarded rate of production of lactic and acetic acids and a 50% increase in the concentration of ammonia-N. The concentration of endotoxin in the silages was 6–7 μg g−1 fresh matter. There was no change in the level of endotoxin during ensiling. High numbers of enterobacteria at the beginning of the fermentation improved the aerobic stability of the silages. There was a lag in the decrease in concentrations of acids and delays in the increase in pH, respiration rate and numbers of yeast and Bacillus spores, in uninoculated silages the pH increased from 4·1 to 5·0 after 8 d of aerobic storage, whereas in inoculated silages a pH of 5·0 was not reached until after 16 d of storage.  相似文献   

6.
Changes in the crude protein (CP) concentration of white clover and perennial ryegrass herbage from a mixed sward were determined on six sampling dates from May to October in each of 2 years. The swards were grown without fertilizer N in an organic farming system and continuously grazed by dairy cows during the grazing season. The annual mean contents of white clover in the dry matter (DM) of the sward were 272·3 and 307·0 g kg−1 in Years 1 and 2. The mean CP concentrations of the white clover and perennial ryegrass herbage were 251·6 and 151·9 g kg−1 DM in Year 1 and 271·9 and 174·0 g kg−1 DM in Year 2 respectively. The CP concentration of the white clover increased significantly during the grazing season from 220·0 to 284·1 g kg−1 DM in Year 1 and from 269·0 to 315·5 g kg−1 DM in Year 2. In the perennial ryegrass herbage the CP concentration increased from 112·2 to 172·6 g kg−1 DM in Year 1 and from 142·7 to 239·5 g kg−1 DM in Year 2. The rate of increase during the season in the CP concentration of the perennial ryegrass herbage was similar to the rate of increase recorded in the white clover herbage.  相似文献   

7.
Seven laboratory-scale experiments were carried out to study the effects of cellulases/hemicellulases on silage fermentation of herbage from mixed swards of timothy ( Phleum pratense ), meadow fescue ( Festuca pratensis ) and red clover ( Trifolium pretense ). Enzyme-treated silage (approximately 3500 HEC units kg−1 grass) reached a low pH sooner, had lower end pH, contained less NH3-N and more lactic acid than did the untreated silage ( P < 0·05). Applied with an inoculant, these effects were even stronger. With easily ensiled crops (experiments 1, 2, 5 and 6) preservation was first of all improved by inoculation of lactic acid bacteria; however, for the low-sugar crops (experiments 3, 4 and 7) enzyme treatment was more significant. The enzymes derived from Aspergillus spp. gave more acetic acid than the enzymes from Trichoderma reesei. The Trichoderma enzymes liberated 4·8 g WSC kg−1 FM gamma-irradiated grass during 60 d at pH 4·3 ( P < 0·05). On average, for all silages enzyme treatment increased the sum of residual sugar and fermentation products by 3·7 g kg−1 FM (21 g kg−1 DM) compared with the silages not treated with enzymes ( P < 0·001). Enzyme treatment increased the instantly degradable part of the feed, but total in sacco and in vitro digestibilities were not affected.  相似文献   

8.
The effect of maturity at harvest of whole-crop barley for ensiling on intake and liveweight gain of dairy steers differing in initial live weight (LW) was evaluated in an experiment over two years. Light (104–120 kg) and heavy (402–419 kg) dairy steers were fed diets containing predominantly whole-crop barley silage harvested at the milk stage [dry matter (DM) content of 284 g kg−1 and neutral-detergent fibre (NDF) concentration of 526 g kg−1 DM] or the dough stage of maturity (DM content of 328 g kg−1 and NDF concentration of 445 g kg−1 DM) and supplemented with up to 1 kg of concentrate. Dry matter intake (g kg−1 LW) was higher for whole-crop barley harvested at the dough stage than at the milk stage of maturity and the difference was greater in heavy than in light steers ( P <  0·001). Liveweight gain was higher and feed conversion ratio was lower for dough-stage compared with milk-stage silage ( P  <   0·05) but there was no interaction with size of steer. Whole-crop barley harvested at the dough stage of maturity promoted higher liveweight gains in dairy steers compared with whole-crop barley harvested at the milk stage due to a higher DM intake.  相似文献   

9.
A randomized block experiment was conducted to compare unwilted and wilted grass silages and the effects of the feed additive monensin sodium on the silage intake and performance of finishing beef cattle. Two regrowths from a predominantly perennial ryegrass (cv. S24) sward were ensiled either without wilting or after field wilting for 3 d (dry matter (DM) concentrations 161 and 266 g kg−1 respectively). Both silages were treated with formic acid (2·6 and 30 litre t−1 respectively) and were well preserved. The silages were offered ad libitum to forty-eight Charolais-cross cattle (thirty-two steers and sixteen heifers, mean initial live weight 351 kg) for 145 d. All animals received 2·2 kg concentrates per head daily and half of those on each silage treatment received in addition 200 mg monensin sodium per head daily. Silage DM intake was 5.04, 504. 5·48, 5·63 ± 0.134 kg d−l; fasted liveweight gain was 0·69, 0·77. 0·64 and 0·73 ± 0.033 kg d−l and carcass gain was 0·47, 0·50, 0·40 and 0·45 ± 0·020 kg d−1 for the unwilted silage without and with monensin and the wilted silage without and with monensin respectively. It is concluded that wilting grass of low DM concentration for 3 d prior to ensiling reduced the performance of finishing beef cattle below that obtained from well-preserved unwilted silage in spite of a higher DM intake being achieved with the wilted silage. The inclusion of monensin sodium in a silage-based diet increased performance without significantly affecting feed intake.  相似文献   

10.
The metabolizable energy (ME) content, digestibility in vivo and chemical composition of fermented and urea-treated whole crop wheat (WCW) forage harvested were measured at different maturities. Over 2 years, twenty-four wheat forages (cv. Slepjner, Hussar and Cadenza) were harvested at 376, 516 and 632 g dry matter (DM) kg−1 in Year 1 and 341, 467 and 544 g DM kg−1 in Year 2 (Cuts 1, 2 and 3 respectively). Forages were conserved in 200−-ι barrels with or without a formic acid-based additive and with urea in Cuts 2 and 3 (20 or 40 g kg−1 DM). Chemical compositions, digestibility in vivo and energy losses in faeces, urine and methane were measured in wethers fed 12 g DM kg−1 live weight. Respective ranges in pH, crude protein, water-soluble carbohydrates, starch and neutral detergent fibre plus amylase (NDFA) contents were 3·8–8·5, 89–394, 2–43, 23–424 and 306–655 g kg−1 DM. ME content, digestible organic matter content (DOMD) and digestibilities of starch and NDFA ranged between 8·4 and 15·7 MJ kg−1 DM, 558 and 708 g kg−1 DM, 0·901 and 0·999 and 0·362 and 0·693 respectively. Predicted methane energy losses were poorly correlated with measured values ( r 2 < 0·45) in both years, but this accounted for less than 0.1 MJ ME kg−1 DM. Significant ( P < 0.05) effects of maturity on the ME/DOMD ratio were observed and ascribed to differences in the extent of fermentation. Therefore, treatment application and maturity exert considerable influence on the ME content of WCW.  相似文献   

11.
A total of eighty-nine big-bale (BB) silages made on commercial farms in South Wales during the autumn in 1983-87 were analysed to discern the effect of dry matter (DM) content on fermentation. Silages were made predominantly in late September, during fine weather from permanent pasture or perennial ryegrass pastures, which had not been grazed for 9 weeks. On average 7·8 ha of pasture were cut; most farmers attempted to pre-wilt grass for an average of 36 h prior to ensilage in individual plastic bags. Silage making was usually completed within 4 d of starting to cut. Silage ammonia-N content was inversely correlated with DM content. The results indicated that a DM content of about 280 g kg−1 was needed to produce well-preserved silage with an ammonia-N content of 100 g (kg total N)−1. Silages were, therefore, generally poorly preserved because the DM increase from wilting was only 2·3 g kg−1 h−1.  相似文献   

12.
In two separate feeding experiments using a total of twenty-four individually housed Ayrshire cows six silages made from perennial ryegrass were offered ad libitum with supplements of concentrates. In Experiment I herbage with a dry matter (DM) concentration of 225 g kg−1 received either formic acid ('Add-F') at the rate of 2·0 litres t−1 or undiluted cane molasses at rates of 10, 20 and 30 litres t−l; the mean daily silage intakes were 9·54, 908, 9·27 and 9·49 kg DM per cow and the daily milk yields, corrected to 40 g fat kg−1, were 23·2, 22·3, 22·8 and 22·9 kg per cow respectively but none of the differences between the four treatments was significant. In Experiment 2 herbage with a DM concentration of 269 g kg−1 received formic acid at a uniform rate of 2·6 litres t−1 either with or without an additional application of molasses at 20 litres t−1; the mean daily silage DM intakes were 8·70 and 9·28 kg per cow and the daily fat-corrected milk yields were 22·2 and 21·9 kg per cow respectively and were not significantly different. In both experiments the effects of the treatments on milk composition were small and not significant. It is concluded that there were no advantages in applying molasses to herbage treated with formic acid, and that the rate of application of molasses to untreated herbage which equated with the formic acid application was 20·30 litres t−l when assessed on the basis of silage composition, intake and milk production.  相似文献   

13.
The agronomic performance and nutritive value of twelve annual and perennial grasses and legumes were analysed in order to define alternatives to local forages for dry-season feeding of ruminants in the Peruvian Andes. There were twelve species and two fertilizer treatments (no fertilizer and a N;P;K fertilizer mainly applied at sowing) in an experiment with a randomized complete block design with three replicates at each of two sites. Plant height, soil cover by forage and weed species, frost damage, dry matter (DM) yield and nutritive value of herbage were evaluated in 2005 and 2006. Among the annual species, Hordeum vulgare L. cv. UNA 80 and × Triticosecale Wittm. had the highest DM yields when fertilized (8226 and 6934 kg ha−1 respectively). Without fertilizer the alternative cultivars had similar DM yields to that of the local forages. Cultivars of Avena sativa L. had lower concentrations of neutral-detergent fibre (NDF) (557 g kg−1 DM) and higher concentrations of predicted net energy for lactation (5·86 MJ kg−1 DM) than the other annual grass species (625 g kg−1 DM and 5·01 MJ kg−1 DM respectively), while the legumes were superior in concentrations of crude protein (277 g kg−1 DM) and NDF (362 g kg−1 DM). Considering the low agronomic performance of the perennial forages, a mixture of fertilized annual grasses and legumes appears the most appropriate approach to meeting the demand for forage of high nutritive value in the Peruvian highlands.  相似文献   

14.
Ensiling of manured crops—effects on fermentation   总被引：1，自引：0，他引：1  
The quality of silage from crops fertilized with cattle manure and an inorganic fertilizer was compared in experiments from 1985 to 1989. Manure was spread either as farmyard manure (FYM, 25t ha−1) or as slurry (20-50t ha−1). Crops were direct cut (approximately 200 g DM kg−1) or wilted (approximately 300 g DM kg−1), precision chopped and ensiled in experimental silos. Silage was treated with 4 kg 85% fonnic acid t−1 fresh matter (FM), an inoculant or no additives. The use of manure, particularly FYM, resulted in more Bacillus spores on crops at harvest compared with fertilized crops. Clostridium spores increased as a result of manuring in 1989 only on FYM-treated crops. Differences in the chemical composition of crops were usually small between fertilizer treatments. The quality of silage from slurry-dressed crops, compared with that of silage from fertilized crops, varied between years. The FYM resulted in reduced silage quality, i.e. high pH values (> 4·5), high ammonia N (> 150 g kg−1 total N) and butyric acid (> 6·3 g kg−1 water) concentrations, and high numbers of Bacillus (105 g−1 FM) and Clostridium spores (105 g−1 FM). The concentration of lactic acid was low (≤ 12 g kg−1 water). Wilting and additives generally improved silage quality and reduced the differences between treatments. However, the efficiency of the inoculant on farmyard manured crops was limited.  相似文献   

15.
An experiment was carried out during 1984 to study the effect of treating grass at ensiling with three commercially available inoculant-type additives (H/M Inoculant, Grass Sile and Siron), formic acid (850 g kg−1; Add-F) or no additive on grass preservation, in-silo loss, intake and animal performance. Primary growth grass ensiled from 28–29 May into concrete-walled covered silos was of high dry matter (DM, 234 g kg−1), water-soluble carbohydrate content (WSC. 212 g kg DM−1) and digestibility (MADF, 250 g kg DM−1).
The untreated silage displayed good preservation and with the exception of the Sirontreated silage which showed significantly lower buffering capacity (Be) and volatile fatty acid (VFA) contents than the untreated silage, the application of inoculant-type additives did not improve silage preservation or decrease in-silo DM losses. The formic acid-treated silage displayed significantly lower Be, water-soluble carbohydrate, ash, ammonia nitrogen (g kg total N−1) and lactate contents than the untreated silage.
After a 133 d storage period, silages were offered to finishing beef cattle for an 84-d period. Cattle offered the silages displayed similar and non-significant daily DM intakes, daily liveweight gains, dressing proportions and daily carcass gains. From this experiment it appears unlikely that any of the additives evaluated will improve animal performance relative to a well-preserved untreated silage.  相似文献   

16.
The effects of sowing date and autumn management of sainfoin ( Onobrychis viciifolia Scop.) were investigated over 3 years in the UK. Replicated plots were sown between April and September in 2003 and 2004. Autumn management treatments were early and late cutting carried out in the establishment year and in subsequent years. Dry matter (DM) yields were measured over 3 years. One harvest was taken from April to July sowings in the establishment year and three harvests in each of the following years. DM yields from sowings in April and May were 2·8 and 3·3 t DM ha−1, respectively, in the establishment year, which were higher ( P  <   0·001) than from sowings in June and July. Sowings from April to July yielded 10·9–12·5 t DM ha−1 in the first full-harvest year, and 9·5–11·5 t DM ha−1 in the following year. Sowings in August and September only gave 5 t DM ha−1 year−1. Early-autumn cutting of an established sward reduced yields of sainfoin at the second harvest in the first and second full-harvest years. Sowing in May had the lowest proportion of weed species (0·06) in the establishment year, and sowing in July had the highest (0·53). Crude protein concentration increased as the seasons progressed from 149·8 to 230·1 g kg−1 DM.  相似文献   

17.
The effect of adding rolled barley on the ensiling characteristics and nutritive value of lucerne and corn ( Zea mays ) forage was studied using 19-1 plastic mini-silos. Other factors evaluated with lucerne were the effect of wilting and added lactic acid bacteria. Rolled barley was added at rates of 0, 50, 100 and 150gkg−1 (wet weight) to lucerne or corn forage. Lucerne was ensiled at 170–330 g kg−1 dry matter (DM) and lactic acid bacteria were applied at 105g−1 wet forage. Addition of rolled barley consistently improved the fermentation of lucerne by lowering the pH and decreasing the concentration of acetate and ammonia nitrogen. Wilting of lucerne had variable effects on fermentation in two experiments. The addition of lactic acid bacteria improved fermentation by decreasing the concentrations of acetate and ammonia nitrogen, improved the nutritive value by increasing the in vitro dry matter digestibility (IVDMD), and was associated with decreased amounts of lactic acid. Corn was ensiled at 250 g kg−1 DM, and added rolled barley had little effect on the fermentation of corn silage. The digestibility of all silages determined in vitro was improved by the addition of rolled barley.  相似文献   

18.
Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10−6 MJ kg MD−1 and 160 and 191 g kg DM−1 respectively. The straw mix had an ME content of 10−1 MJ kg DM−1 and CP content of 134 g kg DM−1. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d−1) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd−1) 163, 155, 163.
Animal performance was, for treatments G, Si and St respectively: milk yield (kg d−1) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg−1) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg−1) 35·3, 32·9, 33·4 (s.e.d. 0·76).  相似文献   

19.
A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.
Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.
The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.
The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg−1. Use of formic acid significantly reduced this requirement to 240 g kg−1 and to 252 g kg−1 for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg−1 is approximately 37 g kg−1 without additive and 30 g kg−1 with formic acid.
It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.  相似文献   

20.
Two silages were made from primary growth perennial ryegrass and ensiled after the application of either formic acid or an enzyme mixture of cellulase and hemicellulase. Silage analysis showed both silages to be well preserved with low pH of 3·70 and 3·62 for the formic and enzyme treatments respectively. Formic acid-treated silage had a higher total amino acid concentration than enzyme-treated silage. The silages were offered to growing steers either as the sole diet or supplemented with rapeseed meal at 60 g or 120 g fresh weight kg−1 silage DM offered, in a 6 × 6 Latin square arrangement.
Non-ammonia nitrogen and microbial nitrogen flows at the duodenum (g d−1) were significantly ( P < 0·05) increased by supplementation of enzyme-treated silage compared with formic acid-treated silage (enzyme, 83·6, 58·7; enzyme + 60 g, 101·7, 75·3; enzyme + 120 g, 112·5, 80·7; formic, 91·9, 63·7; formic + 60g, 88·3, 67·9; formic + 120 g, 95·5, 67·1) respectively. Efficiencies of microbial protein synthesis were increased for supplemented enzyme-treated silage diets and values were reduced for supplemented formic acid-treated silage diets compared with the silage only diets (enzyme, 27·9; enzyme + 60 37·7; enzyme + 120 g, 38·6; formic, 33·7; formic + 60g, 31·2; formic + 120 g, 28·8). Total amino acid flow at the duodenum increased with supplementation of both silages; however, microbial amino acid flow increased significantly ( P < 0·05) with supplementation of enzyme-treated silage compared with formic acid-treated silage diets. Significantly greater amounts of cystine, methionine, alanine, valine and aspartic acid entered the small intestines of animals receiving supplemented enzyme silages compared with supplemented formic acid silages.  相似文献   

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