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1.
In the course of searching for cytotoxic terpenoids from medicinal plants in China, two new eudesmane sesquiterpenoids, 5α-hydroxy-13-methoxy-7αH,11αH-eudesm-4(15)-en-12,8β-lactone (1) and 1β-hydroxy-7αH,11αH-eudesm-4(15)-en-12,8β-lactone (2), along with fourteen known sesquiterpenoids were isolated from the whole plant of Carpesium divaricatum. The structures of new compounds were determined using spectroscopic methods, including IR, HRESIMS, and 1D and 2D NMR spectroscopy. The cytotoxicity of selected sesquiterpene lactones against human oral epidermoid carcinoma (KB), human breast cancer (MCF-7) and human hepatoma (HepG-2) cells was also evaluated by MTT method. 相似文献
2.
The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1-3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC(50) values of 3.14μM and 4.74μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC(50) values of 11.81μM and 25.80μM for HBsAg and HBeAg respectively. 相似文献
3.
Thirteen flavone glucosides from the herb of Euphorbia humifusa were isolated and elucidated. Among them, five compounds including apigenin-7-O-β-d-glucopyranoside (2), apigenin-7-O-(6′′-O-galloyl)-β-d-glucopyranoside (3), luteolin-7-O-β-d-glucopyranoside (7), luteolin-7-O-(6′′-O-trans-feruloyl)-β-d-glucopyranoside (8) and luteolin-7-O-(6′′-O-coumaroyl)-β-d-glucopyranoside (9) showed anti-HBV activity in vitro. The structure–activity relationship showed that the parent structure was closely relevant to the anti-HBV activity of these compounds (agigenin > luteolin > quercetin). It was found that the number of glucoside in the structure may significantly influence their activities (flavone monoglucoside > flavone diglucoside) and cytotoxicity (flavone > flavone monoglucoside > flavone diglucoside). In addition, the substitution of acyl group on glucoside may be important to keep the anti-HBV activities of these compounds (galloyl > feruloyl > coumaroyl). 相似文献
4.
A new guaianolide and a new eudesmanolide were isolated from Lactuca tatarica, as well as eight known sesquiterpenoids. The new compounds were elucidated on the basis of spectroscopic methods including IR, HRESIMS, 1D and 2D NMR, and the known compounds were established by comparing their physical data with those of the corresponding compounds in the literature. 相似文献
5.
《Fitoterapia》2013
Activity-guided fractionation of seeds of Solanum indicum for anti-HBV activity led to the isolation of two novel coumarinolignoid alkaloids (indicumines A–B, 1–2) and two new coumarinolignoids (indicumines C–D, 3–4), together with four known coumarins (5–8). Their structures were established on the basis of spectroscopic data. The two novel coumarinolignoid alkaloids shows anti-HBV activities through specifically inhibiting the secretion of HBsAg in HepG2.2.15. 相似文献
6.
Three new phenolic glycosides, curculigosides F–H (1–3), were isolated from rhizomes of Curculigo orchioides Gaertn. Their structures were elucidated based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (HSQC, COSY, and HMBC). Curculigosides F–H (1–3) were evaluated for their anti-HBV activity in vitro using the HBV transfected Hep G2.2.15 cell line. Compound 1 exhibited weak activity with an IC50 value of 2.08 mM on hepatitis B virus (HBV) e antigen (HBeAg) secretion of the HepG2.2.15 cell line. 相似文献
7.
Three new sesquiterpenoids, 1α-(3″-ethyl-cis-crotonoyloxy)-8-angeloyloxy-3β,4β-epoxy-bisabola-7(14),10-diene (1), 7β-angeloyloxy-14-hydroxy-notonipetranone (2) and 1α-hydroxy-7β-(4-methylsenecioyloxy)-oplopa-3(14)Z,8(10)-dien-2-one (3) were isolated from ethanolic extract of the dried flower buds of Tussilago farfara L., along with nine known sesquiterpenoids (4-12). All of these compounds were evaluated for their effect on the inhibition of nitric oxide (NO) production induced by lipopolysaccharide in macrophage cell line RAW 264.7 and exhibited inhibitory activity on NO production in a dose-dependent manner. 7β-(4-Methylsenecioyloxy)-oplopa-3(14)E,8(10)-dien-2-one (8) was proved to be the best among these tested sesquiterpenoids with an IC(50) value of 10.80 μM. 相似文献
8.
Yuan Cao Nin-Hua Tan Ji-Jun Chen Guang-Zhi Zeng Yun-Bao Ma Yong-Ping Wu He Yan Jie Yang Lai-Feng Lu Qiang Wang 《Fitoterapia》2010
Three new flavones named 5-carboxymethyl-4′,7-dihydroxyflavone (1), its ethyl ester (2) and butyl ester (3) were isolated from the herb Selaginella moellendorffii Hieron., together with ten known compounds. Their structures were elucidated on the basis of spectroscopic and chemical analysis. Selected compounds were evaluated for their anti-HBV and cytotoxic activity. Among them, compounds 2 and 3 displayed inhibitory activity in vitro on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line with IC50 values of 0.17 mg/ml and 0.46 mg/ml, and on HBV e antigen (HBeAg) secretion with IC50 values of 0.42 mg/ml and 0.42 mg/ml, respectively. Compounds 7, 8, 10 and 12 exhibited selective cytotoxicity against the three human cancer cell lines tested. 相似文献
9.
Two new guaiane-type sesquiterpenoids containing an interesting epoxy unit, daucuside (1) and daucusol (2) were isolated from the fruits of Daucus carota L. Their chemical structures were elucidated on the basis of MS, NMR spectroscopic analyses coupled with chemical degradation and they were also evaluated for the cytotoxic effects against two human gastric cancer cell lines BGC-823 and AGS. 相似文献
10.
《Fitoterapia》2014
A new caryophyllane derivative, caryophyllenol A, and a new germacrane derivative, isovolvalerenal D, together with 11 known sesquiterpenoids, were isolated from a petroleum ether partition of the roots and rhizomes of Valeriana amurensis. Structure elucidation of caryophyllenol A and isovolvalerenal D was accomplished on the basis of various spectroscopic techniques including HRESIMS and 2D NMR analyses. The structure of caryophyllenol A was further confirmed by X-ray crystallography and using quantum-chemical ECD calculation adopting TDDFT method. Caryophyllenol A and other eight sesquiterpenoids were evaluated for sedative activity with the model of Drosophila melanogaster, and eight of them showed the effect of prolonging the total sleeping time (TST) of D. melanogaster, displaying significant sedative action. 相似文献
11.
《Fitoterapia》2013
Bioassay-guided phytochemical investigation on Piper longum resulted in the isolation of two new compounds. By analyses of the MS, IR, UV, 1D and 2D NMR data, the two new compounds were identified as 3β, 4α-dihydroxy-1-(3-phenylpropanoyl)-piperidine-2-one (1) and (2E, 4E, 14Z)-6-hydroxyl-N-isobutyleicosa-2,4,14-trienamide (2). In vitro anti-HBV bioassay demonstrated compound 1 possessed remarkable activity suppressing the secretion of HBeAg in Hep G2.2.15 cell line, with an IC50 value of 0.21 mM and SI value of 16.4. 相似文献
12.
《Fitoterapia》2014
Six new 14-noreudesmane sesquiterpenoids, nicotabacosides A–F (1–6), along with five known sesquiterpenoids (7–11), were isolated from the leaves of Nicotiana tabacum. The structures of compounds 1–6 were elucidated as isorishitin 3-O-β-d-glucopyranoside (1), rishitin 3-O-β-d-glucopyranoside (2), rishitin 2-O-β-d-glucopyranoside (3), 1, 6-dehydro-rishitin 3-O-β-d-glucopyranoside (4), 2-hydroxyl-ligudentatol 3-O-β-d-glucopyranoside (5) and oxyglutinosone 3-O-β-d-glucopyranoside (6) based on extensive spectroscopic analyses (HRESIMS, UV, IR, 1D and 2D NMR). Their absolute configurations were determined by X-ray single-crystal diffraction and comparison of their electronic circular dichroism (ECD) spectra. 相似文献
13.
《Fitoterapia》2013
There are 350,000 hepatitis B virus (HBV) carriers all over the world. Chronic HBV infection is at a high risk of developing liver cirrhosis and hepatocelluar carcinoma (HCC), and heavily threatened people's health. Two kinds of drugs approved by FDA for anti-HBV therapy are immunomodulators (interferon α, pegylated-interferon α) and nucleos(t)ide analogues (lamivudine, adefovir dipivoxil, entecavir, telbivudine, and tenofovir disoproxil fumarate). These drugs have been proved to be far from being satisfactory due to their low specificity, side effects, and high rate of drug resistance. There is an urgent need to discover and develop novel effective anti-HBV drugs. With vast resources, various structures, diverse biological activities and action mechanisms, as well as abundant clinical experiences, botanical agents become a promising source of finding new anti-HBV drugs. This review summarizes the recent research and development of anti-HBV agents derived from botanical origin on their sources and active components, inhibitory effects and possible toxicities, as well as action targets and mechanisms, and also addresses the advantages and the existing shortcomings in the development of botanical inhibitors. This information may not only broaden the knowledge of anti-HBV therapy, and offer possible alternative or substitutive drugs for CHB patients, but also provides considerable information for developing new safe and effective anti-HBV drugs. 相似文献
14.
枯萎病是油桐毁灭性病害。中国广泛种植的油桐有三年桐和千年桐,三年桐易感枯萎病,千年桐抗枯萎病。为探讨三年桐与千年桐在枯萎病应答过程中的差异,在对油桐枯萎病灾区调研的基础上,进行枯萎病病原菌的分离、鉴定,并进一步探讨三年桐、千年桐接种枯萎病病原菌后超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的变化。结果表明:油桐枯萎病病原菌为尖孢镰刀菌,接种试验显示千年桐在接种尖孢镰刀菌后SOD、POD、CAT较三年桐均呈现较高活性,在接种后SOD、POD活性先升高后降低,CAT活性升高并维持高活性,MDA含量变化不明显;三年桐SOD活性和MDA含量均先升高后降低,POD活性先降低再升高,CAT活性先降低后升高又降低。千年桐作为抗病种,其抗病性可能与其本身具有较高的SOD、POD、CAT活性有关,并且和病原菌感染后酶活上升有关;三年桐为易感病种,接种后SOD、POD、CAT活性有变化,但仍不能抵御病原菌的危害。 相似文献
15.
Three new lignans, erythro-strebluslignanol (1), threo-7'-methoxyl strebluslignanol (2) and erythro-7'-methoxyl strebluslignanol (3), together with twelve known compounds were isolated from the n-butanol and chloroform fractions of the heartwood of Streblus asper. Their structures were elucidated through extensive spectroscopic methods, including MS and 2D NMR experiments (HMQC and HMBC). The stereochemistry at the chiral center was determined using CD spectra, as well as analysis of coupling constants and optical rotation data, respectively. Primary bioassays showed that 6-hydroxyl-7-methoxyl-coumarin (5) and ursolic acid (10) showed anti-HBV activities, with IC(50) values of 29.60 μM and 89.91 μM for HBsAg at no cytotoxicity, and IC(50) values of 46.41 μM and 97.61 μM for HBeAg at no cytotoxicity, respectively. 相似文献
16.
《Fitoterapia》2013
Three new secoiridoids, swermacrolactones A–C (1–3), together with fourteen known compounds were isolated from Swertia macrosperma. Their structures were elucidated based on extensive spectroscopic analyses (IR, UV, MS, 1D and 2D NMR). By anti-HBV assay on the Hep G 2.2.15 cell line in vitro, the most active compound, luteolin (9), inhibited the secretion of hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) with IC50 values of 0.02 and 0.02 mM, respectively. 相似文献
17.
18.
Nikolaos M. Fyllas Panayiotis G. DimitrakopoulosAndreas Y. Troumbis 《Forest Ecology and Management》2008
The aim of this study is to determine the competing regeneration and expansion patterns of two co-occurring pine species (Pinus brutia, Pinus nigra ssp. pallasiana), in a transitional montane Mediterranean zone. We measured the regeneration density of all woody species in 102 randomly located stands along an altitudinal gradient on the island of Lesbos, Greece. Individuals of pines were assigned to different size classes. Topographic factors (altitude, aspect, and soil depth) and light availability (through hemispherical photographs) were measured for each stand. Statistical analyses were applied to explore the effect of each factor on recruitment density of the competing pine species, and to elucidate patterns of interaction. Canopy openness was the most important parameter controlling the recruitment of P. brutia, while the regeneration density of P. nigra was mainly related to canopy openness and heat load. An idiosyncratic response of the recruitment vigour of the two species was identified along gradients of shade and drought stress. The decline in P. nigra recruitment density with drought conditions underlines threats to its population maintenance even in the absence of fire. On the other hand P. brutia seems to be a stronger invader in transitional zones. The studied species could be considered typical representatives of the two most widely distributed pine functional types across the Mediterranean basin, and our results agree with the theoretical ability of such species to maintain and expand their populations. 相似文献
19.
Because of their high growth rate and tolerance to bare soil, two exotic Acacia species, Acacia auriculiformis and Acacia mangium, have been commonly planted in degraded areas of South China. With their large canopies and ability to fix nitrogen, the two Acacia species have also been considered to act as nurse plants for understory plants. The current study clarified the nursing effects of the Acacia species by comparing microclimate characteristics and physiological traits of native plant seedlings at three sites: under the canopies of the each Acacia species and on bare land (open site). Although the sites were not replicated, the results indicated that adult trees of both Acacia species can facilitate native species, but that A. mangium has greater facilitating effects due to greater temperature buffering, radiation reduction, and nutrient amelioration. In response to facilitation, three species (Castanopsis hystrix, Michelia macclurei, and Manglietia glauca) with different shade-tolerant traits growing under Acacia canopies expressed distinct adaptations. For the three species, the chlorophyll fluorescence curves of rETR and ΔF/Fm′ were higher under A. auriculiformis and on the open site than under A. mangium. The maximum quantum yield in PSII(Fv/Fm) in diurnal changes of the three species showed that all the Fv/Fm values were between 0.70 and 0.84 and that the Fv/Fm values were mostly higher under A. mangium than on the open site or under A. auriculiformis. Total chlorophyll content and both chlorophyll a and b contents in the three species were higher under the Acacia species than on the open site, while chlorophyll a/b ratio was higher on the open site. In contrast, the carotenoid content in C. hystrix and M. macclurei was lower under the two Acacia species than on the open site, while the opposite was true for M. glauca. The results demonstrate that the adaptation of the understory species to abiotic environmental factors is not restricted to a single mechanism but apparently involves a group of interrelated, adaptive suites. And also these adaptations were species-specific and especially related to their shade tolerance. 相似文献
20.
Patrick Vallet Céline Meredieu Ingrid Seynave Thierry Bélouard Jean-François Dhôte 《Forest Ecology and Management》2009
Species choice is potentially an important management decision for increasing carbon stocks in forest ecosystems. The substitution of a slow-growing hardwood species (Quercus petraea) by a fast-growing conifer plantation (Pinus nigra subsp. laricio) was studied in central France. Simulations of carbon stocks in tree biomass were conducted using stand growth models Fagacées for sessile oak and PNL for Corsican pine. The changes in soil carbon were assessed using the Century model and data from two European soil monitoring networks: 16 km × 16 km grid and RENECOFOR. Carbon in wood products was assessed with life cycle analysis and lifespan of final products. However, only carbon stocks and their variation were accounted for: effects of energy-consuming materials or fossil fuel substitution are excluded from the analysis. To compare the growth of these two types of forest stands, an important part of the study was to assess the productivity of both species at the same site, using National Forest Inventory data. 相似文献