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近几年，零散发生于兽医临床上羊只突然死亡的病例，经笔者深入调查及实验室病原分离，将其确诊为此病是由腐败梭菌和魏氏梭菌所引起。 相似文献
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二重PCR方法鉴别气肿疽梭菌和腐败梭菌 总被引：1，自引：1，他引：0
用包含16S～23S rDNA间隔区和23S rDNA的部分序列作为气肿疽梭菌特异性标志,以α毒素部分序列作为腐败梭菌特异性标志,建立了鉴别气肿疽梭菌和腐败梭菌的二重PCR方法。结果显示：气肿疽梭菌C54-1株扩增出大小为509 bp的条带,腐败梭菌C55-1株、C55-16株均扩增出大小为148 bp的条带,均与预期吻合;而产气荚膜梭菌C57-1株、C59-37株,肉毒梭菌C62-4株,诺维梭菌C61-4株均未扩增出任何条带。扩增产物的测序结果进一步证实了本方法的特异性。菌株的生物学特性试验结果也符合相应气肿疽梭菌和腐败梭菌的特点。本研究所建立的二重PCR方法可用于气肿疽梭菌和腐败梭菌的快速鉴定。 相似文献
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刘家森 《畜牧兽医科技信息》2003,19(9):55-56
魏氏梭菌病,又称兔产气荚膜梭菌(A型)病,是兔的一种急性致死性腹泻病,其发病率、死亡率均高,对养兔业的危害极大,造成巨大的经济损失。1 病原:魏氏梭菌即产气荚膜梭菌,一般可分为A、B、C、D、E、F六型。兔的魏氏梭菌病主要由A型引起,少数为E型。A型魏氏梭菌为革兰氏阳性大杆菌,两端稍钝圆,无鞭 相似文献
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笔者从某猪场疑似猪魏氏梭菌病病例中分离到一株魏氏梭菌,为了研究其生物学特性及其发病机理,对分离菌进行了形态培养、生化试验、动物实验等鉴定,鉴定结果为C型魏氏梭菌.药敏试验表明本株魏氏梭菌对青霉素、磺胺药敏感性较高. 相似文献
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猪魏氏梭菌病的诊断与防治 总被引：1，自引：0，他引：1
<正>猪魏氏梭菌病由产气荚膜梭菌引起的一种传染病,又称为猪梭菌性肠炎、猪传染性坏死性肠炎、仔猪肠毒血症,俗称仔猪红痢。被农业部列为多种动物共患的二类疫病。临诊上多以秋末冬初尤其是 相似文献
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4CL（4-coumarate： coenzyme A ligase）是木质素合成途径关键酶,已被证明在生物和非生物胁迫、机械损伤抗性等生物过程中具有重要作用,但与柠檬酸分泌相关的耐铝功能还没有报道。本研究选择丹波黑大豆Gm4CL2,利用RT-PCR技术克隆其全长编码序列,蛋白质序列多重序列比对和进化树分析不同物种间的亲缘关系,农杆菌介导浸花法和叶盘法分别遗传转化拟南芥和紫花苜蓿,qRT-PCR技术检测基因的表达水平。序列分析结果发现,Gm4CL2全长编码序列为1668 bp,该基因编码555个氨基酸,为双子叶植物Ⅰ类 4CL。Real-time PCR结果显示,50 μmol·L-1 AlCl3 （pH 4.5）特异诱导Gm4CL2在丹波黑大豆幼苗0~2 cm的根尖组织表达;过表达Gm4CL2拟南芥,在铝处理条件下其根尖AtMATE、AtSTAR1和AtSTAR2表达量显著上调（P<0.05）。Al3+胁迫条件下,过表达Gm4CL2拟南芥根相对伸长量、根尖SOD、POD活性和柠檬酸分泌量显著高于野生型,根尖伊文思蓝和铬天青S染色以及Al3+、ROS、MDA含量显著低于野生型（P<0.05）;过表达Gm4CL2紫花苜蓿根相对伸长率、根尖柠檬酸分泌量和生物量显著高于野生型,根尖Al3+含量、伊文思蓝染色显著低于野生型（P<0.05）。细胞壁成分分析表明,Al3+胁迫和非胁迫下,过表达Gm4CL2拟南芥根尖果胶、咖啡酸、阿魏酸比野生型显著降低（P<0.05）;Al3+胁迫下,过表达Gm4CL2拟南芥根尖木质素含量显著降低,但是4-香豆酸含量显著升高（P<0.05）。上述结果表明,Gm4CL2为耐铝基因,该基因通过促进细胞壁修饰和柠檬酸分泌提高拟南芥和紫花苜蓿的耐铝性。 相似文献
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象草4CL基因片段的克隆及RNAi 表达载体构建 总被引：1，自引：0，他引：1
象草是热带和亚热带地区广泛栽培的多年生资源植物。为探讨华南象草４-香豆酸：ＣｏＡ 连接酶（4CL）基因下调表达对木质素合成的影响,本研究设计１对特异引物,ＰＣＲ 扩增获得一段长为３７４ｂｐ的干扰片段。通过ＴＯＰＯ 克隆,将该片段克隆到载体ｐＣＲ ／ＧＷ／ＴＯＰＯ ,构建了入门克隆载体ｐＥＮＴＲ-４CL;再经过ＬＲ 反应使ｐＥＮＴＲ-４CL上的干扰片段进入目的载体ｐＣＢ２００４Ｂ 上,形成表达载体ｐＣＢ２００４Ｂ-４CL,最后通过冻融法将其转入到根癌农杆菌ＥＨＡ１０５中。菌液ＰＣＲ 结果表明ＲＮＡｉ质粒已成功转入农杆菌,为进一步利用ＲＮＡｉ技术研究４CL基因的功能奠定了基础。 相似文献
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Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep 
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下载免费PDF全文 Longfei Xiao Junjie Hu Xingxu Zhao Liangli Song Yong Zhang Weitao Dong Quanwei Zhang Youji Ma Fadi Li 《Reproduction in domestic animals》2018,53(5):1142-1148
Melatonin is an important factor involved in regulating reproduction; it is synthesized enzymatically by the sequential action of melatonin‐synthesizing enzymes, arylalkylamine N‐acetyltransferase (AANAT) and hydroxyindole‐O‐methyltransferase (HIOMT), and exerts its biological functions mainly through receptor‐mediated action. To evaluate the expression of melatonin, two melatonin‐synthesizing enzymes (HIOMT and AANAT), and membrane receptors (MT1 and MT2) in oestrous corpus luteum (CL) and CL verum of sheep (Ovis aries), we performed ELISA, qRT‐PCR, western blotting and immunohistochemistry. The quantitative results showed that melatonin, HIOMT and AANAT levels in the CL verum were significantly higher than those in oestrous CL (p < 0.05), whereas MT1 and MT2 exhibited no change between the oestrous CL and CL verum (p > 0.05); moreover, the localization results showed that HIOMT, AANAT, MT1 and MT2 were mainly expressed in large luteal cells (LLCs). In summary, the above results suggested that sheep CL has potential for the synthesis of melatonin; meanwhile, they also suggested that CL is one of the targets of melatonin. These results provide not only a basis for whether sheep CL can synthesize melatonin but also provide a reference for further study on the mechanism of melatonin in the CL. 相似文献
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Tzu-Yin Lin Rachael Thomas Pei-Chien Tsai Matthew Breen Cheryl A. London 《Veterinary immunology and immunopathology》2009,127(1-2):114-124
Studies using the currently available malignant canine mast cell lines and bone marrow-derived cultured mast cells (BMCMCs) have provided an in-depth understanding of normal and neoplastic canine mast cell biology. However, many of the currently available malignant canine mast cell lines possess limitations, including loss of cell surface markers and inability to bind canine IgE. We have recently generated a novel mast cell line, CL1, from an 11-year-old spayed female Labrador retriever diagnosed with systemic mastocytosis and neoplastic effusion. The CL1 cells express KIT, Fc?RI, CD44, CD45, CD14, CD11a, CD11b and CD18 as well as chymase. Interestingly, these cells express wild-type KIT, with no evidence of autophosphorylation, but are able to proliferate independently without the addition of exogenous stem cell factor (SCF), KIT ligand. However, stimulation of CL1 cells with SCF induces KIT phosphorylation promoting cell proliferation. The CL1 cells retain functional properties of mast cells, degranulating in a dose-dependent manner in response to both IgE cross-linking and chemical stimulation. Lastly, cytogenetic evaluation revealed several recurrent tumor-associated chromosome copy number imbalances in the CL1 line. In summary, the CL1 cell line possesses phenotypic and functional properties similar to those found in canine BMCMCs, and will likely be a useful tool to study mast cell biology, factors regulating transformation of mast cells, cytogenetic abnormalities in mast cell tumors, and novel preclinical therapies. 相似文献
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C型肉毒梭菌毒素灭鼠剂试验与灭鼠效果的研究 总被引：4，自引：2，他引：2
对Ｃ型肉毒梭菌干燥毒素的ＬＤ５０，耐药性，蓄积中毒，致畸，毒饵残效期，保存期及灭效方面进行了实验研究，结果为高原鼠兔ＬＤ５０６．７０μｌ／ｋｇ，ＳＬＤ５０１．６７μｌ／ｋｇ，ＬＤ５０９５％置信限３．４７～１１．７０μｌ／ｋｇ小白鼠ＬＤ５０１７０．７０μｌ／ｋｇ，ＳＬＤ５０４．９１μｌ／ｋｇ，ＬＤ５０９５％置信限１０３．００～２４７．００μｌ／ｋｇ，高原鼠兔摄食系数为１．０７，校正灭洞率８６．２１％ 相似文献
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黄芪多糖和香菇多糖对vAMV感染雏鸡淋巴细胞化学发光的影响 总被引：4，自引：0，他引：4
３日龄雏鸡人工感染ｖＡＭＶ并分别注射黄芪多糖（ＡＰＳ）和香菇多糖（Ｌｅｎ）后,于１０,１７,２４,３１日龄检测实验雏鸡的淋巴细胞化学发光（ＣＬ）变化。结果表明：ＡＰＳ对ｖＡＭＶ感染雏鸡脾脏和法氏囊淋巴细胞的ＣＬ有显著影响;Ｌｅｎ对ｖＡＭＶ感染雏手脏和胸腺淋巴细胞ＣＬ值有显著影响。实验结果提示,ＡＰＳ和Ｌｅｎ抗ＡＭＶ及其肿瘤的作用机理可能与其增强淋巴细胞氧自由基代谢有关。 相似文献
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T. J. Rieger C. T. de Oliveira J. Q. Pereira A. Brandelli 《British poultry science》2017,58(3):329-335
1. Feathers are recalcitrant protein-rich wastes produced in huge amounts by poultry processing for meat production. Hence, feather bioconversion and protease production by Bacillus sp. CL18 were investigated.2. Bacillus sp. CL18 demonstrated a remarkable feather-degrading potential. Through cultivations on feather broth (10 g l?1 feathers), 94.5% ± 3% of whole feathers were degraded after 4 d. Increases in soluble protein contents were observed and protease production was maximal also at d 4. This strain produced diverse proteolytic enzymes during growth.3. Crude protease displayed optimal activity at 55°C (50–62°C), pH 8.0 (7.0–9.0) and a low thermal stability. Proteolytic activity increased in the presence of Ca2+, Mg2+, Triton X-100, Tween 20 and dimethyl sulphoxide. Inhibition profile indicated that crude protease contains, mainly, serine proteases. Enzyme preparation hydrolysed mainly casein and soy protein isolate.4. The keratinolytic capacity of Bacillus sp. CL18 at moderate temperatures (30°C) might be appropriate for feather conversion, resulting in protein hydrolysates and proteolytic enzymes. Proteases are postulated to be added-value products that can be obtained from such a bioprocess. 相似文献
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LH and PGF(2alpha) are the principal luteotrophic and luteolytic hormones in domestic animals, however, it is becoming increasingly apparent that intra-ovarian factors can modulate luteal function. For example, the insulin-like growth factors (IGF-I and -II) can regulate ovarian function, and have direct effects on ovarian cells. An important role for the IGFs in regulating ovarian function is suggested by the multiple effects of IGFs on both follicular and luteal steroidogenesis. Expression of mRNA encoding IGF-I, IGF-II and the type 1 IGF receptor has also been detected in the ruminant CL and is suggestive of autocrine/paracrine roles for both IGF-I and -II in the regulation of luteal function. The actions of the IGFs are further modulated by their association with specific binding proteins (IGFBPs), which regulate the transport of IGFs and their presentation to specific receptors. IGFBPs have been detected in the CL of domestic animals, and inhibitory effects on IGF-I-stimulated progesterone production have been demonstrated. The rapid cyclical changes in luteal growth and regression are associated with rapid changes in vasculature. The principle angiogenic factors include the fibroblast growth factors (FGFs), vascular endothelial growth factor (VEGF) and the angiopoietins (Ang). Other locally produced factors include cytokines such as TNF-alpha and IL-1beta. One such factor is monocyte chemoattractant protein (MCP-1), which increases after exogenous PGF(2alpha). An influx of macrophages takes place in the CL around luteolysis, possibly in response to MCP-1 release, but these changes are not observed in cattle when luteolysis is inhibited. In conclusion locally produced factors are important in the control of luteal function, although their roles have yet to fully elucidated. 相似文献