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《中国兽医杂志》2019,(8)
分析不同血清型/生物型沙门菌全基因组序列筛选出鸡白痢和鸡伤寒沙门菌特异性基因组序列,设计两对引物,建立双重PCR方法鉴别检测鸡白痢和鸡伤寒沙门菌不同生物型,并进行初步的临床应用。双重PCR方法结果显示,鸡白痢沙门菌显示417 bp条带,鸡伤寒沙门菌显示417 bp和636 bp两个条带,而阴性对照未出现条带,与预期设计相符。双重PCR体系对56株不同血清型沙门菌鉴定结果与细菌学分离的血清型鉴定结果完全一致,说明本试验建立的双重PCR体系特异性良好,应用上述方法检测鸡场疑似20份临床样本,结果发现8株鸡白痢沙门菌阳性,1株鸡伤寒沙门菌阳性。上述结果表明,已建立双重PCR方法特异性检测鸡白痢和鸡伤寒沙门菌。本试验为鸡白痢和鸡伤寒不同生物型沙门菌的检测提供了一种简洁、敏感、特异的新方法。 相似文献
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根据鸡白痢沙门菌与鸡伤寒沙门菌的rfbS基因在第237和第598位碱基的不同，设计和合成了等位基因特异性PCR引物，建立了快速检测鸡白痢沙门菌的PCR方法，并应用该方法对鸡白痢沙门菌临床分离样品进行了PCR鉴定。结果显示，该PCR方法能够特异性地鉴定鸡白痢沙门菌，检测灵敏度达100PgDNA。对35个经常规方法鉴定的鸡白痢沙门菌分离株应用等位基因特异性PCR方法进行鉴定，鉴定出33株鸡白痢沙门菌，符合率为94．3％。表明，建立的等位基因特异性PCR方法能够准确而快速地鉴定鸡白痢沙门菌。 相似文献
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鸡志贺氏菌感染的血清流行病学调查 总被引：3，自引：0，他引：3
利用研制的鸡志贺氏菌平板凝集抗原及微量平板凝集试验，对我国河南、山东等不同地区35家鸡场具有拉稀病史的670只鸡血清灭活样品进行了检测。通过检测对鸡志贺氏菌病的发病率、流行地区、品种，日龄及其混合感染等进行血清流行病学调查。结果表明：我国部分地区存在有鸡志贺氏菌感染，其血清抗体阳性率较高，达28．3％（155／547）～33．7％（226／670），已远远超过鸡白痢鸡伤寒（1．4％）。目前该病在我国部分地区已广泛流行，不同品种和日龄的鸡均可发生，但血清阳性率存在显著差异，其中固始鸡和罗曼鸡血清阳性率高于其他品种，幼雏血清阳性率高于成鸡。鲍氏血清型阳性率高于痢疾型，并以二者混合感染为主，而该病与鸡白痢鸡伤寒的混合感染很少发生。 相似文献
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陕西关中地区鸡白痢现状调查 总被引：3，自引：1，他引：2
对陕西关中地区鸡场的生产状况及鸡白痢沙门菌感染现状进行调查，随机选取20个鸡场，采集血清305份，采用血清平板凝集试验进行鏊定，并进行分离菌株药敏和动物治疗试验。结果显示，陕西关中地区鸡白痢沙门菌感染严重，阳性率为29％，其中父母代鸡场4个，血清65份，阳性率为20％，商品代鸡场16个，血清240份，阳性率为31％，商品代鸡场比父母代鸡场阳性率高出11％；咸阳、杨陵阳性率为24％，而宝鸡为31％，武功为35％。分离菌株对卡那霉素、恩诺沙星、氟哌酸、头孢噻肟高敏，用以上高敏药物对患病鸡群进行治疗，有良好效果。 相似文献
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禽伤寒和鸡白痢分别是由鸡伤寒和鸡白痢沙门菌引起的禽类的两种不同的败血性传染病。一个多世纪以来,科学家们一直努力在家禽养殖业中控制这两种疾病,但是防控效果不佳或气候条件适于鸡伤寒和鸡白痢沙门菌传播的国家,禽伤寒和鸡白痢仍然是养殖业面临的一个严重问题。过去的15~20年出现了大量有关这两种沙门菌的遗传和免疫学信息,使得人们能更好地了解这两种沙门菌及其与宿主的互作。然而,在了解这两种沙门菌病理学等基础知识的同时,还需要有创造性的思维,以解决当前存在的问题,并研发出控制禽伤寒和鸡白痢沙门菌感染的新方法。 相似文献
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为调查河北地区鸡源沙门菌及其血清型的流行及分布情况,并分析其的致病性,本研究于2018年～2021年在河北省7个地区34个养鸡场共收集631份发病鸡肛拭子和62份病料样品共计693份,分别接种普通琼脂培养基分离细菌并纯化后经革兰氏染色镜检;将分离菌分别接种不同的培养基培养,并对分离菌经生化及16S r RNA基因的PCR及测序鉴定,随机选择32条测序序列经NCBI的BLAST比对,并采用DNAStar软件分析该基因的同源性。采用玻片凝集法鉴定分离菌的血清群及血清型并统计各血清群与血清型在河北不同地区的分布。结果显示,从693份病料样品中共分离到238株沙门菌,除3株未定群外,其余分离菌分属于A、B、C、D、E、F 6个血清群,其中D群最多达69.7%(166/238);238株沙门菌分属于7个不同的血清型和未定型,依次为鸡白痢沙门菌(91/38.2%)、甲型副伤寒沙门菌(43/18.0%)、鸡伤寒沙门菌(40/16.8%)、肠炎沙门菌(39/16.4%)等。血清群与血清型分布的统计结果显示,除张家口地区流行A群血清群外,其余地区流行的血清群均为D群;张家口地区流行鸡白痢沙门菌;秦皇岛地... 相似文献
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斑点免疫金渗滤法检测鸡白痢/鸡伤寒沙门氏菌抗体的应用研究 总被引：2，自引：0，他引：2
将鸡伤寒沙门氏菌LPS抗原包被于渗滤盒检测区（T区），针对沙门氏菌O9抗原单克隆抗体3-47-0包被在质控区（C区），用胶体金标记鸡伤寒沙门氏菌LPS抗原，建立了一种抗原夹心法快速检测鸡白痢、鸡伤寒沙门氏菌抗体的斑点免疫金渗滤法（DIGFA）。DIGFA比玻板凝集试验（PAT）灵敏度高，人工感染鸡试验表明在感染后第7d即可从32只鸡中的7只检测到抗体，在时间上早于PAT。722份现场血清样品的检疫结果表明，DIGFA的阳性检出率稍高于PAT，阳性样品抗体几何平均滴度DIGFA大于PAT法（P〈0．05）。DIGFA的结果得到细菌分离试验的验证。这些结果表明DIGFA快速、灵敏、准确，为鸡伤寒和鸡白痢的检疫提供了一个新的有效手段。 相似文献
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K H Hinz G Glünder S Rottmann M Friederichs 《Berliner und Münchener tier?rztliche Wochenschrift》1989,102(6):205-208
In 40 cases salmonellae of the serovar Salmonella (S.) gallinarum were culturally isolated from domesticated gallinaceous birds submitted for diagnostic purposes in the period of 1979-1989. On the basis of the cultural and biochemical features found 35 of them could be assigned to the biovar Pullorum and 5 to the biovar Gallinarum. Of 35 isolates of the biovar Pullorum, 29 were isolated from pure bred chickens of small fancy-exhibition type flocks, 4 from floor-housed adult brown hybrid laying hens and one each from broiler chicks and pheasant chicks (Phasianus colchicus). Acute to subacute courses of the pullorum disease were observed in the 4 flocks of brown hybrid hens. Of 5 isolates of the biovar Gallinarum, 4 were isolated from adult brown hybrid laying hens kept in battery cages and one from floor-housed brown hybrid pullets of the laying type. First cases of fowl typhoid occurred early in the summer of 1988. The disease was characterized by a peracute course in the 4 flocks of brown laying hens and by a more acute course in the pullet flock. The primary source of the fowl typhoid producing organisms was not elucidated. 相似文献
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Serological response of chickens to infection with Salmonella gallinarum-S. pullorum detected by enzyme-linked immunosorbent assay. 总被引：1，自引：0，他引：1
The serological response to Salmonella pullorum and S. gallinarum infection in chickens was studied with an indirect enzyme-linked immunosorbent assay (ELISA). In broiler chickens, a more virulent strain of S. pullorum produced a significantly lower serum IgG titer than did a less virulent strain. In laying hens, the serum and egg-yolk IgG titers were very similar. In chickens infected with S. gallinarum, high IgG titers persisted for 30 weeks. In chickens reinfected with this strain, each reinfection was followed by transitory increases in IgG lasting no longer than 2 weeks. Serum samples from Brazil taken from a laying flock with evidence of fowl typhoid showed much higher antibody levels than did those from three uninfected flocks. Using lipopolysaccharide as the detecting antigen, infections caused by these salmonellae could be differentiated from those caused by other groups. Incorporation of the appropriate flagella antigen in the ELISA allowed differentiation between infections caused by S. pullorum and S. enteritidis. 相似文献
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Salmonella pullorum in the common pheasant (Phasianus colchicus) 总被引：1，自引：0，他引：1
In 1996, pullorum disease due to Salmonella enterica serovar Gallinarum biovar pullorum (Salmonella pullorum) was diagnosed in pheasants on a gamebird rearing enterprise in south-west Scotland. The gross pathology and bacteriological findings are described, as are the results of screening for S pullorum on the site in 1997. The causal organism was readily isolated from the lung, liver, yolk sac and heart blood on direct culture, but less readily from the digestive tract or by the use of selective media. The bacteria recovered from the pheasants were identified as S pullorum phage type 7, a phage type previously associated with pheasants rather than domestic fowl, and the organisms were most probably introduced to the site by the movement of carrier pheasants. 相似文献
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Nebbia P Tramuta C Ortoffi M Bert E Cerruti Sola S Robino P 《Schweizer Archiv für Tierheilkunde》2007,149(9):403-407
The presence of enteric Helicobacter species was investigated in poultry (n=130) and in pet and ornamental birds (n=50) using a PCR sequencing method which permits the differentiation of many Helicobhacter species derived from animal tissues. All samples were of Italian origin, except for 21 Guinea fowl from a French flock. About 80% of poultry (chickens, laying hens, Guinea fowl) were positive to Helicobacter DNA. H. pullorum was most frequently (62.1%) identified whereas H. pylori and 3 H. sp. hamster B strains were seen in only 3 cases each. Pet and ornamental birds were all negative. H. canadensis was found in all Guinea fowl from a French farm. This is the first report on the occurrence of this bacterium in poultry. 相似文献
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Basnet HB Kwon HJ Cho SH Kim SJ Yoo HS Park YH Yoon SI Shin NS Youn HJ 《Avian diseases》2008,52(1):156-159
Fowl typhoid is a disease of adult chickens and is caused by Salmonella Gallinarum infection via the alimentary tract. The experimental reproduction of fowl typhoid per os (PO) requires artificial conditions to minimize the effect of gastric acid, and several Salmonella serovars have been known to be transmitted via the respiratory route. Therefore, we have hypothesized the existence of a respiratory route for Salmonella Gallinarum infection and have attempted to reproduce fowl typhoid via intratracheal challenge. In accordance with our hypothesis, the intratracheal challenges of Salmonella Gallinarum reproduced exactly same lesions as fowl typhoid and induced higher mortality and morbidity than those of the PO challenge. Therefore, this study represents the first reproduction of fowl typhoid via respiratory route, and our findings may be useful for understanding the transmission of Salmonella Gallinarum in the field. 相似文献
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Early in the summer of 1988 two flocks each of them consisted of about 7500 brown laying hens of a heavy hybrid line were affected by fowl typhoid. The birds have been bought at the age of 20 weeks and housed in two buildings placed close together in one farm. The disease started in flock I at the age of 26 weeks and in flock II at the age of 36 weeks. In repeated trials amoxicillin was effective in the treatment of fowl typhoid when given in the drinking water for ad libitum consumption over a 4-7 days period; however relapse occurred 3-4 days after withdrawal of the drug. All the hens were slaughtered 5 days after termination of the last therapy. In spite of the treatment 18.3% of the hens in flock I and 14.3% of those in flock II died within the observation period of 47 days and 22 days respectively. Egg production was not affected. The source of the fowl typhoid producing organisms could be not elucidated. 相似文献
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Kang MS Kwon YK Jung BY Kim A Lee KM An BK Song EA Kwon JH Chung GS 《Veterinary microbiology》2011,147(1-2):181-185
Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum cause fowl typhoid and pullorum disease in avian species, respectively, and have been of considerable economic importance to the poultry industry in parts of the world. The definitive diagnosis of these diseases can be made only by isolation and identification of the causative agent. However, rapid identification of biovars Gallinarum and Pullorum is not easily feasible due to their common antigenic structure and genomic sequence similarity. We developed a duplex polymerase chain reaction (PCR) assay to identify and discriminate between strains of biovars Gallinarum and Pullorum. Duplex PCR primers were designed to target polymorphic regions of glgC and speC genes showing multiple mutations in the sequenced S. enterica subsp. enterica serovar Gallinarum 287/91 genome and were applied to the specific identification of biovars Gallinarum and Pullorum. Boiled lysates of 131 reference and field strains of Salmonella and other related Gram-negative bacteria were tested to validate the duplex PCR assay. All strains of biovars Gallinarum (n=53) and Pullorum (n=21) tested were correctly identified based on this assay (100% sensitivity) while the other strains (n=57) were PCR negative (100% specificity). These results demonstrate that a highly accurate biovar-specific duplex PCR assay can be performed for the rapid identification and discrimination of biovars Gallinarum and Pullorum from field isolates. 相似文献
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为了解贵州省规模化鸡场鸡白痢流行现状,2017—2019年选取31个规模化鸡场抽检1340份鸡血清样品,采用鸡白痢抗体平板凝集试验,结合鸡场生物安全措施及净化情况,开展了鸡白痢血清学调查。结果显示:鸡白痢平均场群阳性率为83.87%,个体阳性率为13.43%;商品场(15.24%)鸡白痢个体阳性率最高,与父母代鸡场差异显著(P=0.044);育成期(18.77%)个体阳性率高于产蛋期(11.83%),差异显著(P=0.002);不同饮水来源、不同饲养方式的鸡白痢个体阳性率间有差异,但不显著(P>0.05)。车辆人员进出场区时消毒、空舍消毒的鸡白痢个体阳性率较低,但不显著(P>0.05),消毒频率越高,阳性率越低;31个调查场点中,引种前开展疫病检测及采取鸡白痢净化控制措施的场点个体阳性率低于未开展净化的场点,差异极显著(P=0.001、<0.001);仅有32.26%(10/31)场点有意愿开展鸡白痢净化。结果表明:贵州省规模鸡场鸡白痢沙门氏菌感染较为普遍,污染面较广;其感染受场点类型、生长阶段影响较大,而受饲养方式和饮水来源影响较小。结果提示:鸡场实施鸡白痢净化时,可以着重考虑场点类型和生长阶段这两个因素;合理设置场点地理位置、加强场点消毒和开展鸡白痢净化,可显著降低鸡白痢流行率,因此应加强宣传,提高规模化鸡场的鸡白痢防控及净化意识,主动开展鸡白痢净化工作,以控制鸡白痢流行。 相似文献