共查询到20条相似文献，搜索用时 78 毫秒
1.
《林业科学》2017,(7)
【目的】对6个耐寒相关调控基因(RAP2.7,ABR1,At HSFA6B,Atb ZIP44,GRXC6和HSP18.2)在耐寒和不耐寒木麻黄种质中精细表达模式进行分析,为深入解析木麻黄耐寒的分子机制提供理论基础。【方法】测定耐寒(ZS7)和不耐寒(HN1)2种短枝木麻黄无性系在-2~-11℃低温胁迫下的相对电导率,基于Logistic方程对处理温度和对应的相对电导率进行拟合,计算低温半致死温度(LT50)。根据前期转录组测序得到的6个耐寒相关调控基因的EST序列设计特异引物,利用逆转录实时荧光定量PCR分析6个基因在-2,-5,-8℃连续3个温度梯度胁迫下的表达差异,以及在-5℃低温下连续胁迫1,2,5,8,16,24,48,72 h后,在耐寒和不耐寒短枝木麻黄无性系中的精细表达模式。基因相对表达量的计算采用2-ΔΔCT法。【结果】在低温胁迫下,耐寒和不耐寒短枝木麻黄无性系的相对电导率存在极显著差异(P0.01),低温半致死温度分别为-5.92℃和-2.87℃。在常温条件下,6个基因在耐寒和不耐寒种质中的相对表达量皆无明显差异。在临近不耐寒种质半致死温度的-2℃下持续2 h后,6个耐寒相关基因在不耐寒种质中的表达被强烈抑制,而在耐寒种质中的表达被激活;在临近耐寒种质半致死温度的-5℃下持续2 h后,不耐寒种质中的各基因被进一步抑制,同时在耐寒种质中的表达亦被强烈抑制;在低于半致死温度的-8℃下持续2 h后,各基因的表达继续受到抑制。对在临近耐寒种质半致死温度的-5℃胁迫下,耐寒种质和不耐寒种质的精细表达模式分析表明,在-5℃低温胁迫下,耐寒种质中6个基因的表达在8 h后开始呈现极显著上调,表达量最高值集中在低温胁迫后的第8,24,48 h;不耐寒种质中6个基因的表达在1~16 h内皆呈现极显著下调,表达量最低值集中在1~5 h内。【结论】在耐寒相关调控基因的表达水平上,耐寒和不耐寒短枝木麻黄对低温的应答机制明显不同。低温激活了耐寒种质中转录因子、ROS家族基因、ROS应答因子等调控基因的增强表达以抵御和适应逆境胁迫,但抑制了在不耐寒种质中的表达,显著降低了不耐寒种质对低温逆境的耐受能力。该研究在一定程度上对于丰富木麻黄适应低温的分子机制,以及耐寒无性系的分子选育都具有一定的参考价值。 相似文献
2.
《中南林业科技大学学报(自然科学版)》2015,(6)
对7个短枝木麻黄种源的离体小枝在人工气候箱中进行梯度低温处理,在25℃,5℃,0℃,-5℃和-10℃温度下处理24 h,重复3次,每个种源的每个处理采集10株苗的枝条,测定了渗透率、丙二醛含量、脯氨酸含量、可溶性蛋白含量,可溶性糖含量等9个耐寒指标。通过半致死温度评价了7个种源的耐寒能力,并通过耐寒指标的特征结合相关分析对耐寒指标进行筛选。运用主成分分析、回归分析和聚类分析对耐寒指标进一步综合分析。结果表明:利用短枝木麻黄的半致死温度对7个种源进行排序,耐寒能力按种源号依次为18402182681811918267181281801518244。用逐步回归的方式建立短枝木麻黄的耐寒回归方程为Y=9.462-0.119Chl4-0.026SP1-0.039MDA1+0.051Chla3-0.008Chla2,(R2=1.000),估计精度大于98%。 相似文献
3.
《西部林业科学》2019,(6)
为了解橡胶树老幼态无性系植株的抗寒生理特性,比较其抗寒能力。以3个橡胶树品种老幼态芽接无性系的一年生休眠枝条为试验材料,通过人工模拟低温胁迫(4℃对照、2℃、0℃、-2℃、-4℃)处理后,测定其相对电导率、丙二醛(MDA)、过氧化氢(H_2O_2)、游离脯氨酸、可溶性糖及可溶性蛋白含量6个指标,分析各指标随温度下降的变化规律,利用隶属函数法综合评价各无性系老幼态植株的抗寒性。结果表明,随胁迫温度的降低,橡胶树老幼态无性系枝条相对电导率均呈上升的趋势;MDA和H_2O_2呈先上升后下降的趋势;多数无性系游离脯氨酸和可溶性糖含量呈"降-升-降"的变化趋势;可溶性蛋白含量呈先上升后下降的趋势。所有幼态无性系相对电导率、H_2O_2含量及可溶性蛋白含量均低于老态无性系;多数幼态无性系丙二醛低于老态无性系;多数幼态无性系游离脯氨酸和可溶性糖含量高于老态无性系。各无性系抗寒性进行综合评价结果表明,3个品种幼态无性系的平均隶属度都远高于老态无性系。参试的橡胶树品种幼态无性系的抗寒性都比老态无性系强。研究结果为橡胶树幼态无性系的利用潜力和栽培方式提供了参考。 相似文献
4.
《山东林业科技》2021,(4)
以南栾(Koelreuteria bipinnata Franch)无性系"滕选"4号、5号为材料,以北栾(Koelreuteria paniculata)为对照,采用人工低温胁迫方法,研究抗寒性差异及生理指标变化。结果表明:随着胁迫温度降低,不同品种枝条相对电导率持续升高;"滕选"4号、5号、北栾低温半致死温度分别为-20.895℃、-18.723℃、-27.549℃;3种栾树的SOD活性、脯氨酸含量、可溶性糖、可溶性蛋白、MDA含量表现出不同的变化且与对照差异显著;2个选育的南栾无性系"滕选"4号、5号抗寒性低于北栾,但能够在山东省范围内正常越冬。 相似文献
5.
《河北林果研究》2020,(2)
为了解不同榆树资源的抗寒特性,以白榆、黑榆、春榆等3个树种的9个无性系1 a生枝条为试验材料,对枝条进行4℃(对照)、-10℃、-20℃、-30℃、-40℃低温处理,测定各无性系枝条的相对电导率、可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、SOD酶活性、POD酶活性、CAT酶活性、丙二醛质量摩尔浓度8个相关指标,比较各生理指标与榆树抗寒性的相关性,并利用隶属函数方法和主成分分析对9种榆树无性系抗寒性进行对比分析和评价。结果表明:不同无性系枝条电解质渗透率、 MDA与抗寒性呈负相关, SOD、 CAT及POD与抗寒性呈正相关;这8个相关指标可以用于评价9个无性系的抗寒性,且各无性系间抗寒性差异显著(P0.05);根据Logistic方程拟合9个榆树无性系枝条的半致死温度在-33℃～-40℃之间,隶函数综合评价对比分析其抗寒性由强到弱为:白榆、黑榆、春榆;无性系之间由强到弱依次为:D1520、 D1507、D1526、 D2、 H1506、 Y1、 B19、 Y3、 D28,该结果与LT50拟合结果一致。 相似文献
6.
【目的】为野杏的引种驯化及繁育栽培提供参考。【方法】以引种到辽西地区的野杏12个无性系和12个家系为研究对象,以引种地西伯利亚杏无性系和家系作为对照,测定了不同种源的引种野杏无性系及家系枝条的含水量,研究了低温胁迫（0、-20、-25、-30、-35、-40℃）下其可溶性糖含量、可溶性蛋白含量、过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、电导率的变化,应用隶属函数法对其抗寒性进行评价,并基于隶属函数值对各无性系及家系进行聚类。【结果】野杏无性系和家系的枝条电导率曲线均符合典型的“S”形曲线,MDA含量、SOD活性、POD活性均表现为先增后减,可溶性糖含量随温度降低呈上升趋势,可溶性蛋白含量随温度降低表现为先增后减,半致死温度为-42.33～-29.93℃。12个野杏无性系的平均隶属函数值为0.14～0.90,12个家系的平均隶属函数值为0.24～0.74。各无性系按其抗寒性由强到弱排序依次为J170、J19、J171、J148、J162、J144、J210、J105、J118、J101、J211、J270、J121,各家系按其抗寒性由强到弱排序依次为1... 相似文献
7.
低温胁迫对金叶白蜡5个生理生化指标的影响 总被引：2，自引：0，他引：2
以金叶白蜡的1a生休眠枝条为试材、白蜡为对照,对不同温度下的相对电导率、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性及可溶性糖和可溶性蛋白含量进行研究。结果表明：随着胁迫温度的降低,金叶白蜡和白蜡的相对电导率均呈“S”型曲线上升;SOD和POD活性则呈先上升后下降的变化规律;可溶性糖和可溶性蛋白含量总体呈上升趋势。对所有指标进行相关性分析,并利用相对电导率结合Logistic方程进行拟合,结果表明：相对电导率、SOD活性、POD活性、可溶性糖含量和可溶性蛋白含量均可作为金叶白蜡的抗寒性鉴定指标;金叶白蜡和白蜡的半致死温度分别为-29．2℃和-32．3℃,金叶白蜡的抗寒性弱于白蜡。 相似文献
8.
9.
《西部林业科学》2019,(6)
为掌握北京地区引种栽培大果榉的抗寒特性,以垂枝榆为对照,6年生大果榉为试材,选取其一年生休眠枝条分别在5℃(CK)、-10℃、-15℃、-20℃、-25℃、-30℃和-35℃下进行低温处理,测定枝条的相对电导率,应用Logistic方程拟合半致死温度(LT_(50));并测定其枝条的丙二醛(MDA)和可溶性糖的含量,过氧化物酶(POD)和过氧化氢酶(CAT)的活性,枝条恢复生长后的萌芽率,研究低温胁迫下大果榉休眠枝条的抗寒性。结果表明:随温度降低,相对电导率整体呈上升趋势;MDA、可溶性糖的含量以及POD和CAT酶活性均呈现先上升后下降的趋势;恢复生长率鉴定植物抗寒能力的方法易受到植物扦插成活难易程度的影响;相关性最高的抗寒指标是相对电导率,其次是MDA和可溶性糖。大果榉和垂枝榆的LT_(50)分别为-33.11℃和-29.83℃,大果榉的抗寒性高于垂枝榆。 相似文献
10.
【目的】筛选适宜甘肃兰州及同类型气候区发展的品质优良、抗寒性好的葡萄新品种。【方法】以10个引进的鲜食葡萄品种‘春光’‘贵妃玫瑰’‘巨玫瑰’‘蜜光’‘晚黑宝’‘无核翠宝’‘浪漫红颜’‘阳光玫瑰’‘晶红宝’和‘SP9715’为研究对象,分析评价盛果期葡萄的单粒质量、单穗质量、香味、可溶性固形物含量、可溶性糖含量、可滴定酸含量、糖酸比、维生素C含量等主要品质指标,测定低温胁迫后各品种1年生休眠枝的相对电导率、可溶性糖含量、可溶性蛋白含量、脯氨酸含量、丙二醛含量,将10个品种枝条在不同低温处理下的相对电导率运用Logistic方程进行拟合,得出各品种的半致死温度。采用主成分分析法对不同品种的果实品质和枝条抗寒力进行综合评价。【结果】随着温度的降低,10个鲜食葡萄品种枝条的相对电导率逐渐升高,可溶性蛋白含量逐渐上升,脯氨酸含量逐渐升高,可溶性糖含量的变化不尽相同,丙二醛含量总体呈现逐渐上升的趋势。在10个品种中：‘阳光玫瑰’的半致死温度最低,为-22.38℃;其次为‘浪漫红颜’和‘春光’,半致死温度分别为-21.96、-20.75℃;‘晚黑宝’的半致死温度最高,为-18.94℃。【结论】在甘肃... 相似文献
11.
Oliveira DF Pereira AC Figueiredo HC Carvalho DA Silva G Nunes AS Alves DS Carvalho HW 《Fitoterapia》2007,78(2):142-145
A screening was conducted with 26 plants collected in the Brazilian southeast region, to identify plant extracts with antibacterial properties against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Initially, the agar diffusion method was employed. Then, those extracts presenting activity were submitted to a broth microdilution assay to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). It was observed that 13 of the tested extracts showed antibacterial activity. The best results were obtained with those from Lantana lilacina and Phyllanthus tenellus. 相似文献
12.
The Eastern Black Sea Region has an extensive flora because of ample rainfall lasting all year. In this study, antimicrobial effects of 74 crude extracts of 22 endemic plants were investigated. Among the 30 active crude extracts, water-insoluble crude extracts from Betula medwediewii, Heracleum platytaenium, Primula longipes, Anthemis cretica ssp. argaea and Centaurea helenioides were the prominent ones with their MIC values. 相似文献
13.
14.
从落叶松梢栖真菌筛选出对落叶松枯梢病病原菌有抑制作用的菌株,对落叶松枯梢病进行生物防治。将自落叶松梢部分离到的11种梢栖真菌与落叶松枯梢病病原菌进行对峙培养,根据被覆盖程度、抑菌率、拮抗系数和防治预试验的结果,综合评价后发现:粪生粪壳、深绿木霉和球毛壳3种梢栖真菌对落叶松枯梢病有良好的拮抗作用。利用这3个菌株在黑龙江省勃利县通天一林场国富沟进行林间防治试验,结果表明:防治效果为粪生粪壳>代森锰锌400倍液>深绿木霉>球毛壳;深绿木霉和粪生粪壳喷洒浓度50%和球毛壳喷洒浓度75%时,真菌多样性最多,梢栖真菌系统较其他浓度更为稳定,与化学防治相比,既达到了防治目的又具有不污染环境的优势。 相似文献
15.
Growth and physiological response of six Australian rainforest tree species to a light gradient 总被引：1，自引：0，他引：1
An understanding of growth and photosynthetic potential of subtropical rainforest species to variations in light environment can be useful for determining the sequence of species introductions in rainforest restoration projects and mixed species plantations. We examined the growth and physiology of six Australian subtropical rainforest tree species in a greenhouse consisting of three artificial light environments (10%, 30%, and 60% full sunlight). Morphological responses followed the typical sun-shade dichotomy, with early and late secondary species (Elaeocarpus grandis, Flindersia brayleyana, Flindersia schottiana, and Gmelina leichhardtii) displaying higher relative growth rate (RGR) compared to mature stage species (Cryptocarya erythroxylon and Heritiera trifoliolatum). Growth and photosynthetic performance of most species reached a maximum in 30–60% full sunlight. Physiological responses provided limited evidence of a distinct dichotomy between early and late successional species. E. grandis and F. brayleyana, provided a clear representation of early successional species, with marked increase in Amax in high light and an ability to down regulate photosynthetic machinery in low light conditions. The remaining species (F. schottiana, G. leichhardtii, and H. trifoliolatum) were better represented as falling along a shade-tolerant continuum, with limited ability to adjust physiologically to an increase or decrease in light, maintaining similar Amax across all light environments. Results show that most species belong to a shade-tolerant constituency, with an ability to grow and persist across a wide range of light environments. The species offer a wide range of potential planting scenarios and silvicultural options, with ample potential to achieve rapid canopy closure and rainforest restoration goals. 相似文献
16.
Euphorbia acanthothamnos, E. macroclada and E. rigida were investigated for their antioxidant activity. The antioxidant potential of extracts of E. acanthothamnos, E. macroclada and E. rigida was evaluated using different complementary antioxidant tests. 相似文献
17.
The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1-3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC(50) values of 3.14μM and 4.74μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC(50) values of 11.81μM and 25.80μM for HBsAg and HBeAg respectively. 相似文献
18.
Ungulate herbivory of regenerating conifers in relation to foliar nutrition and terpenoid production 总被引：1，自引：0，他引：1
Owen T. BurneyDouglass F. Jacobs 《Forest Ecology and Management》2011,262(9):1834-1845
Ungulate browsing greatly influences regeneration dynamics of some forest ecosystems, yet the relationship between browse susceptibility and foliar chemistry of forest tree seedlings is not well understood. We applied field fertilization (15N-9P-10K controlled-release fertilizer at 0, 20, 40, and 60 g per seedling) and investigated how subsequent changes in terpenoid production and foliar nutrition influence ungulate browse preference for Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco), western hemlock (Tsuga heterophylla Raf. Sarg.), and western redcedar (Thujaplicata Donn ex D. Don) seedlings across four sites in northwestern Oregon, USA. Fertilization increased foliar N concentration of all three species, but above-ground growth of only Douglas-fir and western hemlock. Foliar monoterpene concentrations for western hemlock and western redcedar also increased at higher fertilization rates, while Douglas-fir monoterpene production was not affected by fertilization. Regardless of monoterpene levels, ungulate browse preference was greater for fertilized western hemlock seedlings. The opposite response, however, was observed for western redcedar at two of the four study sites where the likelihood of browse was greater for non-fertilized than fertilized seedlings. Differences in browse preference among species may depend on the type and amount of individual monoterpenes manufactured in response to fertilization. Western redcedar produce α- and β-thujone, oxygenated monoterpenes known to promote gastroenteritis and possibly inhibit microbial rumen activity, which were absent from Douglas-fir or western hemlock. Higher concentrations of α- and β-thujone associated with increasing fertilizer rate provide a plausible explanation as to why ungulates preferred non-fertilized western redcedar. Our results illustrate species-specific adaptation in browse avoidance and selective ungulate browsing behavior of individual trees as linked to foliar chemistry. 相似文献
19.
Emile S. Gardiner Magnus Löf Joseph J. O’Brien John A. Stanturf Palle Madsen 《Forest Ecology and Management》2009
Efforts in Europe to convert Norway spruce (Picea abies) plantations to broadleaf or mixed broadleaf-conifer forests could be bolstered by an increased understanding of how artificial regeneration acclimates and functions under a range of Norway spruce stand conditions. We studied foliage characteristics and leaf-level photosynthesis on 7-year-old European beech (Fagus sylvatica) and pedunculate oak (Quercus robur) regeneration established in open patches and shelterwoods of a partially harvested Norway spruce plantation in southwestern Sweden. Both species exhibited morphological plasticity at the leaf level by developing leaf blades in patches with an average mass per unit area (LMA) 54% greater than of those in shelterwoods, and at the plant level by maintaining a leaf area ratio (LAR) in shelterwoods that was 78% greater than in patches. However, we observed interspecific differences in photosynthetic capacity relative to spruce canopy openness. Photosynthetic capacity (A1600, net photosynthesis at a photosynthetic photon flux density of 1600 μmol photons m−2 s−1) of beech in respect to the canopy gradient was best related to leaf mass, and declined substantially with increasing canopy openness primarily because leaf nitrogen (N) in this species decreased about 0.9 mg g−1 with each 10% rise in canopy openness. In contrast, A1600 of oak showed a weak response to mass-based N, and furthermore the percentage of N remained constant in oak leaf tissues across the canopy gradient. Therefore, oak photosynthetic capacity along the canopy gradient was best related to leaf area, and increased as the spruce canopy thinned primarily because LMA rose 8.6 g m−2 for each 10% increase in canopy openness. These findings support the premise that spruce stand structure regulates photosynthetic capacity of beech through processes that determine N status of this species; leaf N (mass basis) was greatest under relatively closed spruce canopies where leaves apparently acclimate by enhancing light harvesting mechanisms. Spruce stand structure regulates photosynthetic capacity of oak through processes that control LMA; LMA was greatest under open spruce canopies of high light availability where leaves apparently acclimate by enhancing CO2 fixation mechanisms. 相似文献
20.
Insecticidal activity of Vitex mollis 总被引：3，自引：0，他引：3
Organic extracts from the leaves of Vitex mollis were assessed for their toxic effect on fall armyworm neonate larvae (Spodoptera frugiperda), an important insect pest of corn. The extracts showed insecticidal and insect growth regulatory activity, being CHCl(3)-MeOH (1:1) extract the most active, also found to be toxic in the Artemia salina test. 相似文献