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1株难以定型的鸭疫里默氏菌分离株 总被引：6，自引：0，他引：6
采用未经过吸收和经过吸收的抗血清，通过凝集试验和沉淀试验对1999年从河北某鸭场分离到的1株鸭疫里默氏菌(编号为C515)进行了研究。玻片凝集试验可将C515鉴定为19型，试管凝集试验表明，C515与19型参考菌株只有单向低度交叉凝集反应；C515不在10型抗血清中凝集，但C515抗血清可与10型参考菌株发生凝集反应；交互吸收可消除C515与10型和19型之间的交叉凝集反应，但不影响同源凝集反应和同源沉淀反应。C515与1～19型中的其他17个型的参考菌株没有可见的凝集反应，c515与1～19型参考菌株均不产生可见的沉淀反应。单独比较c515与1～19型参考菌株的关系，可将它鉴定为不同于1～19型的血清型，但该菌株还与10型的4个亚型菌株存在不同程度的交叉反应，尤其与亚型4(代表菌株为C598)存在高度交叉凝集反应和清晰的交叉沉淀反应；若将C515定为10型的亚型，又不符合10型分离株的共性。因此，该菌株属于难以定型的一类，可将它暂时鉴定为C598菌株的一个变异株。 相似文献
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用3种分型方法研究鸭疫里默氏菌的血清型 总被引：6，自引：0，他引：6
用玻片凝集试验、试管凝集试验和琼脂扩散沉淀试验3种方法，检测了鸭疫里默氏菌的19个参考菌株和部分分离株与同型和异型抗血清的反应。结果表明，3种分型方法具有很好的相关性，但在检测异型菌株之间的交叉反应时表现出不同。玻片凝集试验适于对大量分离株的快速筛选，但不能作出准确定型。以各型参考菌株为参照，根据试管凝集试验可将被检菌株分型，但当分离株与一个以上血清型的抗血清产生差异较小的凝集效价时，则难以定型。琼扩试验也适合于对分离株进行进一步检测，但要求制备较高效价的抗血清。对血清进行吸收可消除3种分型试验中的所有交叉反应，并制备出单因子血清。但这些单因子血清只是相对于已知血清型具有特异性，某些分离株虽然只与某型单因子血清发生凝集反应，仍可能属于新的血清型或某个已知型的亚型。 相似文献
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鸭疫里默氏菌6型、12型与16型之间的交叉反应 总被引：8，自引：1，他引：8
鸭疫里默氏菌6型和12型仅在玻片凝集试验中存在较弱的交叉反应，这一交叉反应可经血清吸收试验得到消除；12型和16型在玻片凝集试验和试管凝集试验中均表现较强的双向交叉反应，但在琼扩试验中表现为较弱的单向交叉反应；6型和16型之间没有交叉反应。12型和16型之间的交叉反应和交叉沉淀反应均可经血清吸收后得以消除，但经过16型参考菌株吸收过的12型抗血清，还与6型菌株存在交叉凝集反应，只有同时用6型和16型菌株吸收，12型抗血清才具有血清型特异性。血清吸收对6型和12型抗血清的同型凝集反应能力和沉淀反应能力没有影响，但对16型抗血清的同源凝集能力和沉淀反应能力均有较大的影响，经、2型参考菌株吸收后，16型抗血清与同型抗原之间的凝集效价下降2个滴度，而且不再形成清晰的沉淀线。结果表明，6型和12型之间、12型和16型之间均存在ab-bc的抗原关系。 相似文献
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鸭疫里氏杆菌2型与17型之间交叉反应的研究 总被引：16，自引：1，他引：15
本文在国内首次分离到17型鸭疫里氏杆菌。2型与17型在玻片凝集试验中存在明显的双向交叉反尖，在试管凝集试验中表现为单向低度的交叉反应，在琼扩试验中无交叉反尖。2型和17型抗血清经过交互吸收后不再与异型菌株发生凝集反应。沉淀反应模式表明，2型分离株与2型参考菌株、17型分离株与17型参考菌株分别具有相同的热稳定抗原。 相似文献
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血清10型鸭疫里默氏菌4个亚型的分析 总被引：8，自引：1，他引：8
采用琼脂扩散沉淀试验，对只与血清10型鸭疫里默氏菌参考菌株H2199的抗血清发生凝集反应的58株分离株进行了抗原性分析。根据沉淀反应模式，可将这些分离株分成4类：第1类是以C2为代表的30株；第2类是以C449为代表的3株；第3类是以C459为代表的6株；第4类是以C598为代表的19株。第1类分离株与H2199具有相同的热稳定抗原模式，其他类型的分离株与各类代表菌株的热稳定抗原具有同一性，但彼此之间以及与H2199之间存在明显的抗原差异。用未经吸收和经过吸收的抗血清经玻片凝集试验和试管凝集试验对H2199和4类分离株彼此之间的抗原关系进行了进一步的分析。以C2为代表的分离株属于真正的10型；以C459为代表的分离株与H2199存在显著的抗原差异，它们除了与H2199拥有共同抗原外，还有自己的特异性抗原成分，因此，H2199与C459之间的抗原关系可描述为ab．bc。类似的抗原关系还分别存在于H2199与C598、C598与C449之间。但是，在试管凝集试验中，分别相对于C449、C459和C459而言，菌株H2199、C598和C449缺乏自己的特异性抗原成分，因此，H2199-C449、C449-C459、C598-C459之间的抗原关系可分别描述为b-bc。基于上述结果，可将只与10型抗血清发生凝集反应的分离株分成4个亚型，分别以菌株H2199(或C2)、C449、C459、C598作为亚型1～4的代表菌株。 相似文献
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抗副鸡嗜血杆菌血清A和C型株所制备的两个血清型单克隆抗体（MAbs），分别对副鸡嗜血杆菌血清型A、B、C中的各型参考株作HI和dot－blotting试验。一种MAb（E5C12D10）为抗血清型A代表株221，另一种MAb（F2E6）为抗血清型C代表株S1。在两种试验中，不同血清型的MAbs可与对应的血清型中的副鸡嗜血杆菌株血凝（HA）抗原反应，而与血清型B代表株91、147均无反应。故这些MAbs可用于dot－blotting或HI试验进行副鸡嗜血杆菌定型。 相似文献
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我国鸭疫里氏杆菌血清型的鉴定 总被引：42，自引：2，他引：42
１９９７年１月－１９９８年３月,从北京市２０个商品鸭场自然病死的北京白鸭和河南省与上海市部分鸭场的樱桃谷鸭分离到２７６株鸭疫里氏杆菌,采用凝集试验和琼脂扩散沉淀试验,对其进行了血清型的研究。其中７０株细菌为１型,６４株为２型,其余１４２株怀１,２,型参考菌株的抗血清发生反应。 相似文献
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An evaluation of agglutination and coagglutination techniques for serotyping of Haemophilus pleuropneumoniae isolates 总被引：4，自引：0，他引：4
A comparative evaluation of rapid slide agglutination, tube agglutination, 2-mercaptoethanol tube agglutination, and coagglutination tests was made for serotyping isolates of Haemophilus pleuropneumoniae. The results indicated that a majority of the isolates could be serotyped by any of these tests. But, it was not uncommon to find isolates which were inagglutinable or poorly agglutinable in homologous sera. Heat treatment of whole-cell suspensions of such isolates was essential to unmask the serotype-specific antigenic determinants; however, in the process of heat treatment, cross-reactive common antigens of minor nature were also exposed. The antibodies involved in such cross-reactions were mainly of immunoglobulin M type, because the cross-reactivities were completely abolished in coagglutination and 2-mercaptoethanol agglutination tests. Thus, both these tests were satisfactory for serotyping inagglutinable mucoid strains. For serotyping strains which were either polyagglutinating or autoagglutinating, agglutination tests could not be used, but the coagglutination test proved to be satisfactory. The coagglutination test was serotype-specific, sensitive, simple, rapid, reproducible, and easier to read and interpret than rapid slide or tube agglutination tests. This test could be used to serotype mucoid, smooth, or rough isolates. 相似文献
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Eight strains of Haemophilus pleuropneumoniae isolated from 8 herd outbreaks of pleuropneumonia in pigs were studied by means of the slide agglutination test, the tube agglutination test, the IHA test and by gel diffusion.The 8 strains were antigenically homogeneous and serologically distinct from serotypes 1 through 7. It is therefore proposed to refer these strains to a new serotype: serotype 8, with strain 405 as the type strain.In addition to the serotype-specific capsular antigens, capsular antigen of serotype 3 (strain 1421) and serotype 6 (strain Femø) could be demonstrated in the 8 strains by means of the IHA test and by gel diffusion analyses. 相似文献
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A total of 100 field isolates of Actinobacillus pleuropneumoniae isolated from lung tissues of pigs with severe pleuropneumonia were serotyped by slide agglutination and precipitation tests. Polymerase chain reactions for apxICA, apxIICA, apxIIICA, apxIBD and apxIIIBD genes were used to determine their genotype prevalence. Serotypes 2 (56 isolates), 5 (28 isolates) and 6 (11 isolates) were the most common; only two isolates belonged to serotype 7, and three were untyped. Among the 97 isolates identified by serotype, 70 had the same apx genes as their respective serotype reference strains, but 27 did not have any of the apx genes present in the corresponding serotype reference strain. Among these 27 isolates, 10 were serotype 2, 12 were serotype 5, three were serotype 6 and two were serotype 7. 相似文献
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Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine 总被引：14，自引：0，他引：14
One hundred and forty-one isolates of Haemophilus pleuropneumoniae from Iowa and Illinois swine were characterized morphologically and biochemically and serotyped by rapid slide agglutination (RSA) and indirect fluorescent antibody (IFA) tests. Hyperimmune antisera were produced in rabbits using inactivated whole-cell suspensions of the reference strains for H pleuropneumoniae serotypes 1 to 7 and strain 202, representing the taxon "minor group." Cross testing of the reference strains and reference antisera indicated the antisera to be essentially serotype-specific, although reactivity of some antisera with heterologous strains was observed. Cultures of the 141 isolates formed adherent or smooth colonies or mixtures of these colony forms. Adherent and smooth colony types were found in all serotypes identified. Microscopic and biochemical characteristics of all isolates were typical of those previously described for H pleuropneumoniae. The overall incidence of H pleuropneumoniae serotypes was serotype 5, 55.3%; serotype 1, 34.0%; serotype 7, 7.8%; and nontypeable, 2.8%. Comparing the 2 test procedures, 87.2% of the isolates could be typed by RSA, and 66.0% could be typed by IFA. Cross-reactions between serotype 4 antisera and serotype 5 and 7 isolates were common with the IFA test. The reactions with serotype 7, but not serotype 5, were eliminated by cross adsorption of serotype 4 antisera. There was good correlation between the 2 test procedures, but RSA was judged to be more specific and sensitive than IFA. 相似文献
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Serological studies of Actinobacillus (Haemophilus) pleuropneumoniae strains of serotype 6 and their antigenic relationship with other serotypes 总被引：3，自引：0，他引：3
Serological tests such as agglutination, coagglutination, precipitation and indirect haemagglutination were used to study the antigenic relationship of reference and field strains of Actinobacillus (Haemophilus) pleuropneumoniae of serotype 6 with reference strains of other serotypes. Both cell-associated particulate and cell-free soluble antigens prepared from unheated and heat-treated bacterial suspensions of reference and field strains of serotype 6 were used in the studies. Species-specific, common antigenic determinants associated mainly with heat-treated particulate antigens of serotype 6 were cross-reactive in tube agglutination tests with almost all the serotypes. The species-specific antigens were of a minor nature because the cross-reactivities were abolished in both 2-mercaptoethanol agglutination and coagglutination tests. Cell-free saline extracts of both unheated and heat-treated suspensions of serotype 6 strains possessed epitopes specific for serotypes 3, 5 and 8 in addition to their own specific determinants. The epitopes were dominant because the reactions of strains of serotype 6 with antisera against serotypes 3, 5 and 8 persisted in almost all the serological tests used. Serotype 6 strains were antigenically closer to serotype 8 than to serotypes 3 or 5. A combination of serological tests such as coagglutination followed by 2-mercaptoethanol tube agglutination and, or, immunodiffusion tests differentiated serotype 6 strains from those of other cross-reacting serotypes. 相似文献
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Rapid detection of Streptococcus suis serotype 2 in weaned pigs 总被引：5，自引：0，他引：5
A Moreau R Higgins M Bigras-Poulin M Nadeau 《American journal of veterinary research》1989,50(10):1667-1671
A survey to detect Streptococcus suis serotype 2 in 1,716 weaned pigs was done in Quebec. Forty-nine sow herds were included in this survey: in 26 herds, S suis serotype 2 had been isolated during the preceding 12 months and in 23 herds (control), the organism had not been detected during a previous study. Swab specimens of the nasal cavity and tonsils of pigs were obtained for bacteriologic culture, and S suis serotype 2 was easily detected by the use of brain-heart infusion agar containing a Streptococcus-selective supplement and 5% goat antiserum raised against S suis serotype 2. After measurement of the diameter of the precipitation zone of 539 isolates, a slide agglutination test was performed to identify the S suis serotype 2 isolates. The mean precipitation zone diameter obtained for group S suis serotype 2 was larger (P less than 0.001) than that for the group designated as "others". With slide agglutination test results as reference and on the basis of discriminant analysis to stimulate detection of S suis serotype 2, 93.1% of all isolates were correctly classified, using the precipitation zone diameter as unique classification criterion. Relative specificity was 94.5% and relative sensitivity was 88.7%. Use of the precipitation zone diameter on a quantitative basis led to the proposal of a simple and reliable technique to screen swine herds for S suis serotype 2 in weaned pigs. Nasal and tonsillar swab specimens were obtained and analyzed concurrently for S suis serotype 2. The organism was found in both sites in only 20.4% of 103 carrier pigs.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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Serological characterization of Haemophilus pleuropneumoniae (Actinobacillus pleuropneumoniae) strains and proposal of a new serotype: serotype 9 总被引：15，自引：0，他引：15
R Nielsen 《Acta veterinaria Scandinavica》1985,26(4):501-512
Ten strains of H. pleuropneumoniae isolated from 10 herd outbreaks of pleuropneumonia were studied by means of the slide agglutination test, the indirect haemaggluitiniation (IHA) test and by gel diffusion. The strains were antigenically homogeneous and serologically distinct from serotypes 1 through 8. It is therefore proposed to refer these strains to a new serotype: serotype 9, with strain CVJ 13261 as the type strain.In addition to the serotype-specific capsular antigens, capsular antigen of serotype 1 (strain 4074) could be demonstrated in the 10 strains by means of gel diffusion analyses.In cross protection studies it was shown that the antigenic determinants shared by serotypes 9 and 1 were unable to yield a sufficient protection against disease. Thus, parenteral immunization with a killed 6-h culture of serotype 9 did not afford an acceptable protection against challenge with serotype 1 since only 3 of the 5 vaccinates were protected. The reverse experiment showed that parenteral immunization with serotype 1 only protected 1 out of 4 vaccinates. 相似文献
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J Mazurová 《Veterinární medicína》1985,30(4):247-254
The method of rapid slide agglutination and coagglutination was tested in the detection of Haemophilus equigenitalis (Taylorella equigenitalis)--the causal agent of contagious equine metritis (CEM). It was demonstrated that both methods were suitable for the serological diagnosis of the species under study. The antisera obtained from rabbits immunized with Haemophilus equigenitalis strains treated in different ways were specific, but with different antibody titres. When cross reactions with other species of microorganisms were verified, the antisera did not react with any of the strains, even after binding them to protein A of the positive strain Staphylococcus aureus--Cowan I. Coagglutination was much more rapid and pronounced than the ordinary rapid agglutination test. It was characterized by a low consumption of specific antiserum. The specific antibodies bound to staphylococci were kept at the temperature of 4 degrees C for several months without losing agglutinin activity. 相似文献