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Thiago M. Costa Alice K. Inoue-Nagata Andreza H. Vidal Simone da G. Ribeiro Tatsuya Nagata 《Plant pathology》2020,69(6):1042-1050
The severe yellowing disease (amarelão) on melon plants is a serious problem in Brazil, although the causative agent remained unknown for a long time. Recently, recombinant isolates of cucurbit aphid-borne yellows virus (CABYV) were reported as the possible causative agents of this disease on melon plants. Although aphids are known to be the vectors of the common type of CABYV isolates, almost no aphid colony was observed in the major melon fields in Brazil with high incidence of the severe yellowing disease. In contrast, whiteflies are often abundant. Based on this observation, the hypothesis of the transmission of recombinant CABYV by whiteflies was evaluated. After thorough transmission experiments, we found that this recombinant CABYV isolate was transmitted by the whitefly Bemisia tabaci MEAM1, but not by Aphis gossipii. Furthermore, the host response by whitefly-based inoculation in cucurbits and other indicator plants showed differences in host range when compared to the common type of CABYV. Due to its transmissibility by the whitefly and the distant relationship of the P3/P5 protein to CABYV, the name “cucurbit whitefly-borne yellows virus” is proposed for this recombinant CABYV. This is the second report of polerovirus transmission by the whitefly B. tabaci, following the report of pepper whitefly-borne vein yellows virus. 相似文献
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Beet western yellows virus (BWYV; genus Polerovirus, family Luteoviridae) is one of the most important viruses causing yellowing disease of many field and vegetable crops. This study isolated different poleroviruses from sugar beet, spinach, radish and brassica in Japan, and identified them as BWYV-JP, Beet leaf yellowing virus (BLYV), Brassica yellows virus (BrYV) and BrYV-R (radish strain) based on host range and molecular analysis. Among over 100 plant species from 19 families inoculated with the vector Myzus persicae, about half of the species in 13 families were infected with some of these viruses. BLYV shared a similar host range to Beet mild yellowing virus (BMYV). These had a much more limited host range than BWYV-JP, which resembled BWYV-USA. The host range of BrYV was similar to that of Turnip yellows virus (TuYV). Phylogenetic analyses at the 5′ portion (replication-related gene) of the genome showed that BLYV, BMYV, BWYV (-JP and -USA) and Cucurbit aphid-borne yellows virus (CABYV) formed one large group, whereas BrYV and TuYV were grouped together. BLYV and BWYV were most closely related to each other, and were more closely related to CABYV than to BMYV. However, at the 3′ end (coat protein gene), BLYV and BWYV-JP formed a distinct group, separated from the BrYV group, which in turn was more closely related to BWYV-USA, BMYV, TuYV and Beet chlorosis virus, a group originating from outside Asia. Thus, this study presents host range differences and phylogeographical relationships of BWYV-like poleroviruses that are distributed worldwide. 相似文献
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A. Chomič M. N. Pearson G. R. G. Clover K. Farreyrol D. Saul J. G. Hampton K. F. Armstrong 《Plant pathology》2010,59(3):429-442
This study, using RT‐PCR, is the first comprehensive assessment since 1991 of a generic detection method for the Luteoviridae. Thirteen Luteoviridae species were detected using three separate sets of low‐degeneracy generic primers with RT‐PCR to amplify 68‐, 75‐ and 129/156‐bp regions of the Luteoviridae coat‐protein gene. Species detected include all members of the genus Luteovirus [Barley yellow dwarf virus (BYDV)‐PAV, BYDV‐PAS, BYDV‐MAV (129 and/or 156 bp amplicons), Soybean dwarf virus, Bean leafroll virus (68 bp amplicon)] and eight of nine species from the genus Polerovirus [Beet western yellows virus, Beet chlorosis virus, Beet mild yellowing virus, Turnip yellows virus, Potato leafroll virus, Cucurbit aphid‐borne yellows virus, Cereal yellow dwarf virus‐RPV (68‐bp amplicon) and Sugarcane yellow leaf virus (75‐bp amplicon)]. These primers were not able to detect Carrot red leaf virus, Sweet potato leaf speckling virus (both belong to unassigned Luteoviridae) and Pea enation mosaic virus‐1 (genus Enamovirus). A synthetic positive control containing all primer sequence priming sites was designed to facilitate this method as a generic tool for use with a variety of host plants. The Luteoviridae primers described in this study present a simple infection‐detection tool of benefit to biosecurity authorities in nursery‐stock surveillance, disease management or outbreak prevention, and may also be useful in detection of as‐yet undiscovered species within the Luteovirus and Polerovirus genera. 相似文献
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Anna Kozłowska-Makulska Jerzy Syller Marek S. Szyndel Olivier Lemaire Salah Bouzoubaa Etienne Herrbach 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(2):337-341
Different field isolates of the ‘beet poleroviruses’ Beet mild yellowing virus (BMYV) and Beet chlorosis virus (BChV) (genus Polerovirus, family Luteoviridae) collected in France and Poland were evaluated for transmissibility from and to sugar beet plants by different aphid species.
In general, both BMYV and BChV were efficiently transmitted by Myzus persicae and by a French clone of Macrosiphum euphorbiae. In contrast, transmissibility of the two poleroviruses by an English clone of M. euphorbiae was evidently weaker, although the aphid samples contained the virus as demonstrated by RT-PCR. None of the BMYV or BChV
isolates was transmitted by Aphis fabae or Myzus ascalonicus. In attempting to correlate biological properties with molecular variations, the RT proteins were sequenced. Some amino acid
point variations, presumably affecting aphid transmissibility, were identified. 相似文献
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A new yellowing disease of cucurbits caused by a luteovirus, cucurbit aphid-borne yellows virus 总被引：3，自引：0，他引：3
H. LECOQ D. BOURDIN C. WIPF-SCHEIBEL M. BON H. LOT O. LEMAIRE E. HERRBACH 《Plant pathology》1992,41(6):749-761
Since 1988, a yellowing disease of melon, cucumber and zucchini squash has been frequently observed in summer and autumn crops in France. Infected plants show yellowing and thickening of the older leaves; symptom intensity differs depending upon cultivar and season, and can be easily overlooked when plants are already infected by mosaic-inducing viruses or other pathogens. The disease is associated with the presence of a virus with spherical particles c. 25 nm in diameter, which is readily transmitted in a persistent manner by the aphids Myzus persicae and Aphis gossypii , but not mechanically. Serological analysis, nucleic-acid-hybridization experiments and host-range studies indicate that the virus is distantly related to, but distinct from, beet western yellows virus (BWYV). We propose to name this virus cucurbit aphid-borne yellows virus (CABYV), and to consider it as a tentative new member of the luteovirus group. CABYV was found to reduce significantly the yields of melon and cucumber by decreasing the number of fruit per plant but not by altering the fruit shape or quality. Preliminary investigations of the epidemiology of CABYV indicate that the virus is common in weeds and in cultivated cucurbits. CABYV was frequently detected in various regions of France, suggesting that it is one of the most prevalent viruses infecting cucurbits in this country. 相似文献
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Aphid-borne viruses are responsible for major cucurbit diseases and hamper the sustainability of crop production. Systematic monitoring can reveal the occurrence and distribution of these viruses, in addition to unadvertised viruses, facilitating the control of diseases. For three consecutive (2018–2020) seasons, the presence of aphid-borne viruses was monitored from a total of 292 samples of watermelon and squash plants that showed yellowing symptoms in three major cucurbit-producing areas (Castilla La-Mancha, Alicante, and Murcia) in Spain. We observed that cucurbit aphid-borne yellows virus (CABYV) was the most common virus found (29%) in the plants from both crops. Likewise, except for squash samples from Castilla La-Mancha and Alicante, watermelon mosaic virus (WMV) was also found (23%) with a relatively high frequency. Furthermore, we observed the exacerbation of bright yellowing symptoms in watermelon plants that was often accompanied by considerable fruit abortion. CABYV was the only causative agent for this new yellowing disease, and two infectious cDNA clones (one from watermelon, CABYV-LP63, and another from melon, CABYV-MEC12.1) were constructed to further compare and characterize this CABYV disease. Based on the full-length genome, both isolates were grouped phylogenetically together within the Mediterranean clade. However, the Koch's postulates tests were only successfully completed for the LP63 isolate, which also showed several amino acid changes and two potential recombination events, as compared to MEC12.1. Remarkably, the LP63 isolate caused more severe symptoms and showed higher RNA accumulation than MEC12.1 in five cucurbit plant species. These results suggest that a novel CABYV variant that causes severe yellowing symptoms may be causing outbreaks in cucurbit crops. 相似文献
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Distribution and properties of geographically distinct isolates of sugar beet yellowing viruses 总被引：1，自引：0，他引：1
M. Stevens N. J. Patron ‡ C. A. Dolby R. Weekes § P. B. Hallsworth O. Lemaire H. G. Smith 《Plant pathology》2005,54(2):100-107
From a total of 261 yellow sugarbeet leaves collected from 10 countries representing three continents, the incidence and distribution of strains of Beet mild yellowing virus (BMYV), Beet chlorosis virus (BChV) and Beet yellows virus (BYV) were analysed using serological and molecular methods. BMYV was found in all countries except Greece, and more frequently in the northern and western areas of Europe, whereas BYV predominated in Turkey, Spain, Greece, the USA and Chile. BChV, originally found in the USA and the UK in 1989, was identified in France, Spain, the Netherlands and Chile. Nine sugar beet poleroviruses, plus a reference isolate of Turnip yellows virus (TuYV, syn. Beet western yellows virus ), were further characterized and compared. Isolates obtained from sugar beet infected this species, but not oilseed rape or lettuce; all isolates except one infected Capsella bursa-pastoris . The coat-protein sequences of these isolates were highly similar, with the consensus sequence representing 89% of nucleotide residues. Within the coat-protein gene, two regions were identified that could represent specific epitopes to which monoclonal antibody BYDV-PAV-IL-1 could bind; this antibody is used to distinguish beet poleroviruses in ELISA. Comparison of the sequences at the 5' end showed that sequence homology existed only between isolates with the same host range. The first sequence data of polerovirus isolates from Chile are presented, showing that the coat protein and the 5' end of their genomes are highly similar to those of BMYV isolates found in Europe. Chilean polerovirus isolates may have been imported from the northern hemisphere in sugar beet breeding material. 相似文献
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R. L. Lamprecht G. Pietersen G. G. F. Kasdorf L. H. Nel 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(1):105-110
A previously uncharacterised plant rhabdovirus, infecting Bermuda grass (Cynodon dactylon) in the North West Province, South Africa, has been found. To determine the morphology and virion size of this virus, embedded
ultra-thin sections of infected plant samples were observed under a transmission electron microscope. The virion distribution
within the cell, its bullet-shaped morphology and its size (240 × 63 nm) indicated that this might be a rhabdovirus of the
genus Nucleorhabdovirus. Degenerate polymerase chain reaction (PCR) primers were designed by alignment of the polymerase gene sequences of several
plant rhabdoviruses in order to identify conserved regions. Standard PCR and sequencing protocols were used to determine a
partial polymerase gene sequence of this virus sample which was then compared to the most closely related sequences available
on Genbank. The analysis indicated that the virus was indeed most closely related to known nucleorhabdoviruses, with the highest
nucleotide sequence similarities being to Maize mosaic virus and Taro vein chlorosis virus (70% and 69.7% respectively). Serological testing indicated that the South African Cynodon rhabdovirus had a close serological relationship with the nucleorhabdovirus Cynodon chlorotic streak virus. 相似文献
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A. Fox A. R. Fowkes A. Skelton V. Harju A. Buxton-Kirk M. Kelly S. M. D. Forde H. Pufal C. Conyers R. Ward R. Weekes N. Boonham I. P. Adams 《Plant pathology》2019,68(3):576-587
Ullucus tuberosus (ulluco) is a tuber-forming species that has become a novel crop in highland and temperate maritime climates. Eight viruses have been previously reported infecting Ullucus, including Andean potato latent virus (APLV), a quarantine virus within the European Union. No reference sequences have been published for the viruses previously described from U. tuberosus. Plants grown in the UK for the internet trade were tested for the presence of quarantine viruses using ELISA and real-time RT-PCR. ELISA positive results were obtained for APLV and multiple other viruses. A similar suite of viruses was detected at a second outbreak site linked to horticultural trade. Virus identification was by high-throughput sequencing (HTS) using a ribosomal RNA (rRNA)-depleted total RNA approach. Analysis of viral contigs indicated the presence of several novel viruses closely related to, but not consistent with, the viruses indicated by ELISA. Further confirmatory testing by real-time RT-PCR indicated that two tymoviruses, tentatively named Ullucus tymovirus 1 and Ullucus tymovirus 2, were more closely related to each other (85% identity), than to APLV or Andean potato mild mosaic virus (63–66% identity). APLV could not be confirmed from either site by either HTS or PCR. A novel tobamovirus (Ullucus tobamovirus 1) was only detected at the initial outbreak site. A novel polerovirus (Ullucus polerovirus 1) and a distinct genotype of Papaya mosaic virus were detected from both outbreak sites. Deploying HTS during a plant health outbreak demonstrates the potential of this approach to give rapid, accurate diagnosis. 相似文献
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Monarda yellows occurring in southern Alberta was found to be associated with a phytoplasma. Using two pairs of universal primers, 16S ribosomal DNA fragments (about 1.5 and 1.2 kb) were amplified separately by polymerase chain reaction (PCR) from DNA samples that had been extracted from infected monarda. No such DNA bands were observed using DNA samples from uninfected monarda. The DNA fragment (1.2 kb) amplified by nested-PCR was analysed and compared with western aster yellows (AY27, Canada), eastern aster yellows (EAY, USA), French hydrangea aster yellows (AYHF), Belgium hydrangea aster yellows (AYHB), clover proliferation (CP, Canada) and potato witches'-broom (PWB, Canada) by means of restriction fragment length polymorphism (RFLP) using endonucleases Alu I, Mse I, Hpa II, Sau 3AI, Kpn I and Rsa I. The results showed that monarda yellows phytoplasma belongs to the aster yellows subclade and is different from CP and PWB. This is the first report of aster yellows phytoplasma infecting monarda. 相似文献
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南瓜蚜传黄化病毒遗传多样性分析 总被引：2，自引：1，他引：1
哈密瓜是新疆重要的经济作物之一。南瓜蚜传黄化病毒(cucurbit aphid-borne yellows virus, CABYV)是世界重要的瓜类黄化病毒,在新疆哈密瓜植株上普遍发生,严重威胁新疆哈密瓜的品质与产量。本研究从新疆阿克苏市随机采集120份哈密瓜叶片样品,经过RT-PCR检测证实38个样品为CABYV阳性,从阳性样品中分离、测序、克隆得到一个新的CABYV分离物。将该分离物与22个来自不同国家的分离物(NCBI)进行核酸和氨基酸序列一致性和系统发育分析,结果显示23个CABYV分离物根据地域差异被划分为2个不同进化群体。进一步对不同CABYV群体进行遗传多样性分析,结果表明CABYV在寄主和地理种群之间存在明显的遗传差异。选择压分析和偏离中立性检验显示CABYV种群近期可能发生了种群扩张或背景选择,并且负向选择可能是CABYV遗传变异的原因之一。本文较全面研究了CABYV的发生、危害与遗传结构及进化机制,可为新疆病毒病防治提供理论指导。 相似文献
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J. A. Herrera‐Vásquez M. C. Córdoba‐Sellés M. C. Cebrián J. A. Rosselló A. Alfaro‐Fernández C. Jordá 《Plant pathology》2010,59(2):240-251
The geographic incidence, genetic diversity and phylogenetic relationships of Melon necrotic spot virus (MNSV) and Olpidium isolates were studied in three cucurbit species from several Latin American and European countries on different collecting dates. Of the 112 cucurbit samples analysed, 69 from Guatemala, Honduras, Mexico, Panama and Spain were DAS‐ELISA‐positive for MNSV. Olpidium bornovanus and O. virulentus infections, and MNSV infections mixed with these Olpidium species, were observed for all these countries. Twenty‐nine MNSV isolates from all the origins where the virus was detected were selected and amplified by RT‐PCR. The resulting RT‐PCR of the p29, p89, p7A, p7B and p42 proteins was used to estimate the genetic diversity and the phylogenetic relationships of the MNSV population. The sequences obtained in this study were compared with the MNSV sequences of the NCBI database, and three groups were recovered by nucleotide composition according to geographical origins: the EU‐LA genotype group (with two subgroups: EU and LA, European and Latin American isolates, respectively), the JP melon genotype group (Japanese melon reference isolates) and the JP watermelon genotype group (Japanese watermelon reference isolates). The genetic diversity in the entire p7A and p7B proteins of MNSV suggests that these coding regions are under strong selective pressure. Additionally, the rDNA‐ITS region was analysed in 40 O. bornovanus and O. virulentus isolates associated with each geographical location and host examined. Phylogenetic analysis showed two groups for each Olpidium species, and these groupings were related to the host from which they were originally isolated. 相似文献
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Symptom expression and levels of the ipomovirus Cucumber vein yellowing virus (CVYV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV) were compared in greenhouse cucumbers in single and mixed infections. Results were contrasted with those obtained for plants infected with the potyvirus Zucchini yellow mosaic virus (ZYMV) in single and in mixed infections with either CVYV or CYSDV. Cucumber showed leaf symptoms of each co‐infecting virus, except for the combination of CYSDV with ZYMV, where the typical CYSDV‐like symptoms of interveinal leaf yellowing were inconspicuous or absent. The progression of CVYV as quantified by real‐time RT‐PCR was similar in plants with single infections and in mixed infection with CYSDV between 15 and 60 days post‐inoculation (dpi). However, CYSDV was detected at significantly enhanced levels in plants when co‐infected with CVYV but not when co‐infected with ZYMV. In the latter case, ZYMV levels were reduced when compared with single infections. During mixed infections of ZYMV and CVYV, the titre levels of the ipomovirus were significantly lower when compared with single infections. Cucumber had reduced plant height, internode length, dry weight and fruit yield, positively correlated with the titre levels of CVYV and not of CYSDV during mixed infections. It is concluded that co‐infections with CVYV enhance the titre of CYSDV, which could have epidemiological significance. 相似文献
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C. G. Orfanidou L. C. Papayiannis P. G. Pappi N. I. Katis V. I. Maliogka 《Plant pathology》2019,68(4):764-774
Cucurbit yellow stunting disorder virus (CYSDV), Cucurbit chlorotic yellows virus (CCYV) and Beet pseudo-yellows virus (BPYV) are whitefly-transmitted criniviruses that cause foliar interveinal yellowing symptoms and result in high economic losses for cucurbit production. CYSDV and CCYV are transmitted by Bemisia tabaci, whereas BPYV is transmitted by Trialeurodes vaporariorum. During 2012–2017, an extensive survey was conducted to identify the viruses causing cucurbit yellows disease in Greece and Cyprus. The study sampled the main cucurbits and alternative hosts in these regions to determine crinivirus incidence, to identify the whitefly species present in the two countries and to characterize molecularly the virus populations. Results showed that CYSDV was the most widespread virus in Greece (49.9%), followed by CCYV (20.3%) and BPYV (18.4%). Bemisia tabaci and T. vaporariorum were identified in 54.5% and 45.6% of whitefly samples, respectively. In Cyprus, CYSDV was predominant (96.7%), followed by CCYV (19.2%), while no BPYV infection was detected. Approximately 15% of weed samples from 17 different species that belong to 12 botanical families were identified as hosts for one or more of these criniviruses. Finally, sequencing of the capsid protein gene of the crinivirus isolates revealed very low levels of genetic diversity, further supporting the genetic stability of crinivirus populations. The results of this long-lasting epidemiological study in two countries of the eastern Mediterranean revealed substantial changes in the relative incidence and distribution of cucurbit-infecting criniviruses and their whitefly vectors over the past 15 years, suggesting the need for adoption of novel management strategies. 相似文献
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Yellows-diseased plants of Crepis setosa (hawksbeard), Knautia arvensis (field scabious), Convolvulus arvensis (field bindweed), Picris echioides (bristly oxtongue), Echium vulgare (blueweed) and Calendula officinalis (pot marigold) collected in central and southern Italy were examined for phytoplasma infection by means of polymerase chain reaction (PCR) technology using universal phytoplasma primers directed to ribosomal sequences. The detected phytoplasmas were characterized and differentiated using restriction fragment length polymorphism analysis of PCR-amplified DNA. The phytoplasma detected in diseased pot marigold plants was identified as a member of the aster yellows group and proved indistinguishable from a strain of the American aster yellows phytoplasma. The phytoplasma identified in diseased field bindweed plants is a putative new type of the stolbur group that differed from the typical stolbur phytoplasma. Phytoplasmas detected in diseased hawksbeard, blueweed and field scabious plants are all putative new members of the sugarcane white leaf group while the phytoplasma detected in diseased bristly oxtongue plants represents a new member of the faba bean phyllody group. For hawksbeard and field scabious this is the first report on the occurrence of phytoplasma diseases, whereas phytoplasmas infecting bristly oxtongue and blueweed have never been characterized before. 相似文献