共查询到18条相似文献，搜索用时 156 毫秒
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NAA、IBA对樱桃砧木(Prunus pseudocerasus Colt)插条的生理、生化代谢和生根的影响 总被引：7，自引：0，他引：7
用NAA、IBA处理樱桃砧木嫩枝和无根组培苗, 测定了插条生根率、生根条数和生理、生化指标的动态变化。结果表明: 经处理后插穗的各项生根指标都得到了显著提高, 其中100 mg/L 浓度的NAA与IBA处理插穗生根率达到了88.3%和85% , 组织培养的试管苗瓶外生根率达89%和87.5%; 嫩枝插穗的可溶性糖、可溶性蛋白质、叶绿素、核酸物质的含量与对照组相比发生了显著的变化: 在不定根原基的诱导期, 插穗叶绿素、核酸、可溶性糖含量显著增加, 可溶性蛋白质含量下降; 在不定根的形成期,插穗的可溶性糖被不定根的形成所消耗而含量显著下降, 但可溶性蛋白质含量逐渐上升; 在不定根形成后插穗具有了吸收外界营养的能力, 故在不定根伸长期叶片中可溶性糖开始积累, 含量上升。这些物质含量的动态变化与插穗生根相关, 说明生长调节剂是通过调节插条内代谢物质的含量来促进插穗的生根。 相似文献
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诱导樱桃度管苗生根的研究 总被引：1，自引：1，他引：0
以甜樱桃和酸樱桃杂种“Ｓ２”的试管苗为试材，研究了ＩＢＡ、蔗糖及暗培养时间对不定根产生的影响。结果表明，暗培养对不定根产生有显著影响，ＩＢＡ和蔗糖的影响作用较小，获得高生根率的最优组合为１／２ＭＳ＋ＩＢＡ０．１ｍｇ／Ｌ＋蔗糖３％培养基、暗培养７天。 相似文献
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以三角枫插穗为试材,采用不同种类、浓度的生长素进行处理,分别于春夏秋3个季节进行扦插繁殖试验,并以夏插生根插穗的韧皮部为材料,研究三角枫扦插生根过程中营养物质含量的动态变化。结果表明:与对照(清水浸泡)比,用100mg·L~(-1) NAA+100mg·L~(-1) IBA浸泡插穗3h生根率最高(77%),生根指数为34.28cm;在插穗生根过程中,该处理与对照插穗韧皮部营养物质含量的动态变化趋势相似,可溶性糖含量的变化趋势为"下降-上升-下降-上升-下降"、可溶性淀粉含量为"下降-上升-下降-上升",可溶性蛋白质含量为"上升-下降-上升";100mg·L~(-1)NAA+100mg·L~(-1) IBA浸泡插穗3h明显提高了可溶性糖和可溶性蛋白质含量、促进了可溶性淀粉含量的降解转化;在缩短不定根发生周期方面,生长素处理无明显作用。 相似文献
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植物生长调节剂对竹节秋海棠插枝生根的影响 总被引：6，自引：0，他引：6
试验观察了植物生长调节剂NAA、IAA、IBA、6-BA、2，4-D等对竹节秋海棠插枝生根的效应．结果表明，不同植物生长调节剂对竹节秋海棠插枝生根的影响不同，同一植物生长调节剂不同浓度对其生根也有所不同。其中NAA以150mg／L(处理插条基部3．5h)效果最好；IAA以1250mg／L(处理茎尖基部2h)效果最好；IBA以200rag／L(处理茎尖基部5h)效果最好；2，4-D以1．5mg／L(处理插条基部4h)效果最好：而6-BA则对竹节秋海棠插枝生根有抑制作用，并且随着浓度的升高而增大，当浓度为3．5mg／L时，所有插条均不生根． 相似文献
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以苹果矮化砧木‘冀砧2号’为试材，研究绞缢、环割压条生根效果和IAA含量及相关指标变化，结果表明：绞缢处理的压条生根率为78.92%，显著高于环割和对照；压条生根过程中，绞缢处理的IAA含量显著高于环割和对照，35 d时出现最大值，为39.93 ng·g-1；不定根诱导期和形成期，绞缢处理的POD、PPO活性显著高于环割和对照，IAAO活性均低于环割和对照；不定根诱导期，绞缢处理的Md PAT1、MdPIN1相对表达量逐渐上升，35 d时相对表达量最高，显著高于对照；25 d时MdYUCCA4相对表达量显著高于环割和对照，MdARF17的相对表达量则显著低于环割和对照。综上，绞缢可显著提高‘冀砧2号’砧木压条生根率和生根指数，生长素IAA对‘冀砧2号’不定根根原基的诱导和促进不定根伸长具有重要作用。 相似文献
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以厚皮甜瓜品种“古拉巴”试管苗为材料研究了不同培养因子对其生根的影响以及瓶外直接扦插生根的可行性。结果表明：古拉巴试管苗生根较难，即使在较适宜的 １／２ ＭＳ＋ＩＡＡ ０．５ ｍｇ／ Ｌ和 １／２ ＭＳ＋ＩＢＡ ０．１ｍｇ／Ｌ培养基上，其生根率也不足８０％，但如将转接的材料先经暗处理３天，则在同样培养基上生根率可达９０％以上．瓶外直接扦插也显示出较好的生根效果，在精心管理并合理使用生长调节剂的条件下，生根率达８１．４％－８８．３％，与组培诱导的结果相近，且成本低，易于移栽成活。 相似文献
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预处理和繁殖环境对榛子带叶绿枝扦插生根影响 总被引：1，自引：0，他引：1
预先在生长季里早期将母株插条基部贴上黑胶带进行插条的局部黄化处理，然后用金属丝将其基部环缢。这种方式可明显提高榛子带叶绿枝扦插生根率，使两同以后生根率从４１．０％提高到８２．０％。发现在不考虑繁殖环境的条件下，这种综合处理胜过单独使用黄化或环缢。而在高湿、通风、弥雾环境中，这种综合处理可使生根率提高９０％。这种方法也增加了主根数量和长度，并且插条基部坏死在这种处理下也是最少的。而在环缢条件下，根系 相似文献
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IBA和发根农杆菌对糠椴嫩枝扦插的影响 总被引：5，自引：0，他引：5
研究了IBA和发根农杆菌处理对糠椴嫩枝扦插生根的影响, 并对其影响生根的机理进行了初步探讨。结果表明: IBA和发根农杆菌处理显著( P < 0.01) 提高了插条生根率, 增加了成活苗的生根数和根长, 明显缩短了生根时间。IBA和发根农杆菌处理还显著( P < 0.01) 提高了插条生根期内源GA3和IAA、可溶性总糖和全氮的含量及IAA /ABA比值, 降低了ABA含量, 对糠椴嫩枝扦插生根有明显的促进作用, 其中以500 mg·kg-1 IBA +发根农杆菌处理的生根效果最好, 插条的生根率、成活苗的生根数、成活苗的根长分别比对照增加了122.08%、65.75%和23.63% , 生根时间比对照缩短了14 d。 相似文献
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为了探明吲哚丁酸(Indole-3-butyric acid,IBA)对辣椒(Capsicum annuum L.)下胚轴不定根发生的诱导机制,采用IBA浸蘸后扦插的方法,研究了外源IBA处理后辣椒下胚轴不定根发生进程、糖类物质转化与不定根发生相关基因表达情况。结果表明:外源IBA诱导辣椒下胚轴不定根发生具有浓度依存效应,150μmol·L~(-1) IBA处理后不定根发生区段长度、不定根数及平均根长分别是对照的3.47倍、3.28倍和1.91倍;150μmol·L~(-1) IBA上调了不定根启动相关基因Ca ARL1和Ca Cyc B1的表达,下胚轴的不定根原基发生区域明显增加;此外,150μmol·L~(-1) IBA还上调了下胚轴不定根发生区段糖代谢相关基因Ca CWI和Ca STP的表达,可溶性糖、淀粉含量及细胞壁转化酶活性显著提高。结果表明适宜浓度IBA处理可以促进辣椒下胚轴不定根发生区段糖类物质的积累和转化,诱导辣椒下胚轴不定根的发生。 相似文献
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B. H. Howard M. S. Ridout 《The Journal of Horticultural Science and Biotechnology》2013,88(6):681-687
There was a positive correlation between shoot length and the proximal diameter of the prepared cutting in stockplant sources of Prunus insititia ‘Pixy’ that had different rooting potential. The precise relationship depended on source, with shoots from a severely pruned micropropagated source, and those developing from the trunks of various stock- plants, usually being thinner for any given length than normal distal cuttings from pruned and non-pruned conventional source stockplants. To a large extent rooting percentage, root numbers and root length increased between sources as the overall ratio of proximal stem diameter to shoot length decreased. Cuttings from the relatively thick, short shoots of the unpruned source were among the poorest rooting, while those from the relatively thin, long shoots from the trunk, and from the micropropagated source, rooted best. This between-source effect reflected the fact that within the less ready rooting sources there was also a negative correlation between the proximal diameter of the cutting and its rooting ability. This relationship was lost progressively as the rooting potential of sources increased, thus giving no opportunity for a trend to occur in the trunk-derived cuttings from the micropropagated source where virtually all cuttings rooted. 相似文献
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《Scientia Horticulturae》2005,103(4):479-488
The effect of the mature leaf retention and the exogenous application of flavonoids (naringenin, quercetin and rutin at 30 μM for 12 h) was studied for adventitious rooting of Ilex paraguariensis cuttings. Softwood cuttings harvested from young 3-year-old plants and adult 10- and 20-year-old plants were rooted under intermittent fog. A strong correlation (r2 = 0.72) between leaf retention and rooting was noted. The highest percentage of adventitious root formation (40%) was obtained when the leaf was artificially removed after 42 days of incubation. This data was supported by the histological analysis which provided anatomical evidence that cuttings have initiated root primordia by 21 days and the regenerated roots emerge through the epidermis after 35 days of incubation. A strong correlation between the position of the leaf and the site of roots regeneration was observed. A 100% of the rooted cutting with a single leaf only formed roots along the leaf axis at the base of the cutting. Quercetin increased the rooting percentage more than three times compared to the control and all flavonoids tested improved the distribution of roots around the stem without impacting the number of regenerated roots per rooted cutting from 20-year-old plants. 相似文献
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Stem cuttings of oregano (Origanum vulgare L.) with pre-formed roots responded to a range of IBA and IAA concentrations with increased root emergence and without damage to the cuttings. No similar promotion of rooting was observed in cuttings lacking pre-formed root primordia. The data are discussed with respect to the differentiation and elongation phases of adventitious root formation. Root emergence from oregano cuttings and the continuity of stem vessels into the growing root are described. 相似文献
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P.J. Wilson 《The Journal of Horticultural Science and Biotechnology》2013,88(4):591-600
SUMMARYA rooting morphogen, arbitrarily defined, can be assumed to induce adventitious roots in woody stem cuttings. Auxins generally promote rooting in herbaceous cuttings and are often considered to limit rooting, but they often have little or noeffect when applied to difficult-to-root woody cuttings. They can move in a polar (basipetal) pathway but the polarity of root emergence equally reflects the activity of the vascular system in general, including the phloem, whose contents have a complex composition. The interactions between a morphogen of vascular origin and potential sites for root initiation arelikely to be dynamic and variables such as flux rate or flux direction could be more relevant than static variables such as composition or concentration. The potential sites are not equally sensitive to the rooting morphogen since each cell hasa unique lineage, ontogeny and position, and a prevailing insensitivity could restrict rooting. The contents of the vascular system are inaccessible to potential sites in the stem, so factors affecting efflux and the subsequent attenuation of the efflux, which depend on activity in the whole cutting, could be critical to rooting ability. No simply defined morphogen can be said to limit rooting, and the chemical identity of the morphogen (if definable) is probably unimportant. 相似文献