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1.
Adzuki bean(Vigna angularis(Willd.) Ohwi Ohashi) is an annual cultivated leguminous crop commonly grown in Asia and consumed worldwide. However, there has been limited research regarding adzuki bean genetics, which has prevented the efficient application of genes during breeding. In the present study, we constructed a high-density genetic map based on whole genome re-sequencing technology and validated its utility by mining QTLs related to seed size. Moreover, we analyzed the sequences flanking insertions/deletions(In Dels) to develop a set of PCR-based markers useful for characterizing adzuki bean genetics. A total of 2 904 markers were mapped to 11 linkage groups(LGs). The total length of the map was 1 365.0 cM, with an average distance between markers of 0.47 cM. Among the LGs, the number of markers ranged from 208(LG7) to 397(LG1) and the total distance ranged from 97.4 cM(LG9) to 155.6 cM(LG1). Twelve QTLs related to seed size were identified using the constructed map. The two major QTLs in LG2 and LG9 explained 22.1 and 18.8% of the total phenotypic variation, respectively. Ten minor QTLs in LG4, LG5 and LG6 explained 3.0–10.4% of the total phenotypic variation. A total of 9 718 primer pairs were designed based on the sequences flanking In Dels. Among the 200 selected primer pairs, 75 revealed polymorphisms in 24 adzuki bean germplasms. The genetic map constructed in this study will be useful for screening genes related to other traits. Furthermore, the QTL analysis of seed size and the novel markers described herein may be relevant for future molecular investigations of adzuki bean and will be useful for exploiting the mechanisms underlying legume seed development.  相似文献   

2.
【目的】利用分子标记技术,构建甘薯遗传连锁图谱,并分析甘薯淀粉含量性状的QTL位点,为高淀粉含量甘薯种质资源利用及甘薯分子标记辅助育种提供理论和实践依据。【方法】以高淀粉含量品种万薯5号为母本、低淀粉含量品种商丘52-7为父本建立杂交群体,利用EST-SSR标记,采用"双假测交"策略和运用Join Map4.0软件,分别构建双亲遗传连锁图谱,并结合F1(2012、2013年)群体表型数据采用区间作图法对淀粉含量性状进行QTL检测。【结果】利用1 679对EST-SSR引物筛选出的1 045对多态性引物检测F1群体的标记基因型,获得了1 418个标记位点。分别对上述获得的父母本多态性标记进行遗传连锁分析,在LOD≥5.0情况下,分别构建父母本的连锁遗传图谱。采用642个标记的多态性位点构建母本连锁群74个,其中,215个标记位点位于连锁图谱上,占标记多态性位点总数的33.5%。每个连锁群上有2—11个标记位点,连锁群长度在0.6—129.4 cM,图谱总长为3 826.07 c M,标记间平均距离为17.80 c M。属于父本的776个标记位点构建了80个连锁群,共有252个标记位点构建在连锁图谱上,占标记总数的32.5%,每个连锁群上有2—24个标记位点,连锁群长度在2.0—156.8 c M,图谱总长为3 955.0 cM,标记间平均距离为15.7 c M。以F1杂交群体构建的遗传连锁图谱,结合2012年、2013年2个环境,利用QTL作图软件MapQTL5.0,采用区间作图法进行分析,共检测到17个与淀粉含量性状相关的QTL,贡献率在8.4%—40.5%。其中qWsc-1、qWsc-2、qWsc-3 3个QTL位于母本万薯5号连锁群上,且在2年环境中均可检测到;14个QTL位于父本商丘52-7连锁群上,qSsc-1、qSsc-2、qSsc-3、qSsc-4、qSsc-8、qSsc-10、qSsc-11、qSsc-12是在2个环境均检测到的QTL。qSsc-5、qSsc-6、qSsc-7、qSsc-9、qSsc-13、qSsc-14是只在1个环境检测到的QTL。标记GDAAS0603在双亲中和2个环境中均同时检测到,这些环境稳定QTL可用于分子标记辅助选择。【结论】分别构建了亲本EST-SSR分子标记连锁群图谱,丰富了构建甘薯图谱的标记类型,定位了17个与淀粉含量相关的QTL位点。  相似文献   

3.
Genetic linkage maps are important for quantitative trait locus (QTL) and marker-assisted selection breeding. The wolfberry (Lycium spp.) is an important food and traditional medicine in China. However, few construction genetic linkage maps have been reported because of the lack of genomic and genetic resources. In this study, a population of 89 F1 seedings was derived from a cross between two heterozygous parents, L. chinense var. potaninii ‘BF-01’ (female) and L. barbarum var. auranticarpum ‘NH-01’ (male), in order to construct a genetic linkage map using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers based on the double pseudo-test cross mapping strategy. The resulting genetic map consisted of 165 markers (74 AFLPs and 91 SSRs) distributed across 12 linkage groups and spanned a total length of 557.6 cM with an average distance of 3.38 cM between adjacent markers. The 12 linkage groups contained 3 to 21 markers and ranged in length from 8.6 to 58.3 cM. Twenty-nine segregated markers distributed in the map were mainly located on LG4 and LG9 linkage groups at P<0.05. This is the first linkage map of Lycium species using SSR and AFLP markers, which can serve as basis for improving genes and selective breeding of the genome assembly.  相似文献   

4.
黄瓜雌性性状的QTL定位分析   总被引：1，自引：0，他引：1  
以黄瓜雌性系D0420×强雄性系D06103的211株F2单株为作图群体,应用SSR分子标记进行多态性筛选,得到与黄瓜雌性性状相关的标记位点21个,分属4个连锁群,连锁群全长为98.5 cM,标记间平均距离4.9 cM,最短的连锁群0.5 cM(LG1),最长的连锁群53.4 cM(LG2);标记间最小的遗传距离0.2 cM,最大的遗传距离25.2 cM;采用复合区间定位分析,检测到与黄瓜雌性性状相关的QTL位点2个,均位于第3连锁群上,距离最近标记的遗传距离分别为2.1和1.4 cM,LOD值分别为50.04和6.48,贡献率分别为15.36%和5.69%.  相似文献   

5.
QTL Analysis of the Oil Content and the Hull Content in Brassica napus L.   总被引：4，自引：0，他引：4  
The QTLs of the oil content and the hull content were analyzed in Brassica napus L. By constructing the linkage map. The F26 RIL population with 188 lines, derived from the cross of GH06 × P147, was used as the mapping population. The SRAP, SSR, AFLP, and TRAP markers were used to construct the linkage map, and the composite interval mapping (CIM) to identify the quantitative trait loci associated with the oil content and the hull content. 300 markers were integrated into 19 linkage groups, covering 1 248.5 cM in total. Seven QTLs were found to be responsible for the oil content with the single contribution to phenotypic variance ranging from 3.73 to 10.46%; four QTLs were found for the hull content with the single contribution to phenotypic variance ranging from 4.89 to 6.84%. The yellow-seeded Brassica napus L. Has the advantage of higher oil content and the hull content has a significant effect on the oil content. In addition, the SRAP marker is good for detecting QTL.  相似文献   

6.
Litchi chinensis Sonn is widely cultivated in subtropical regions and has an important economic value. A high-density genetic map is a valuable tool for mapping quantitative trait loci (QTL) and marker-assisted breeding programs. In this study, a single nucleotide polymorphism (SNP)-based high-density linkage map was constructed by a genotyping-by-sequencing (GBS) protocol using an F1 population of 178 progenies between two commercial litchi cultivars, ‘Ziniangxi’ (dwarf) and ‘Feizixiao’ (vigorous). The genetic map consisted of 3027 SNP markers with a total length of 1711.97 cM in 15 linkage groups (LGs) and an average marker distance of 0.57 cM. Based on this high-density linkage map and three years of phenotyping, a total of 37 QTLs were detected for eight dwarf-related traits, including length of new branch (LNB), diameter of new branch (DNB), length of common petiole (LCP), diameter of common petiole (DCP), length of internode (LI), length of single leaf (LSL), width of single leaf (WSL), and plant height (PH). These QTLs could explain 8.0 to 14.7% (mean=9.7%) of the phenotypic variation. Among them, several QTL clusters were observed, particularly on LG04 and LG11, which might show enrichment for genes regulating the dwarf-related traits in litchi. There were 126 candidate genes identified within the QTL regions, 55 of which are differentially expressed genes by RNA-seq analysis between ‘Ziniangxi’ and ‘Feizixiao’. These DEGs were found to participate in the regulation of cell development, material transportation, signal transduction, and plant morphogenesis, so they might play important roles in regulating plant dwarf-related traits. The high-density genetic map and QTLs identification related to dwarf traits can provide a valuable genetic resource and a basis for marker-assisted selection and genomic studies of litchi.  相似文献   

7.
基于PCR技术的谷子分子标记遗传图谱构建   总被引：1，自引：0，他引：1  
【目的】构建一张基于PCR技术的谷子分子标记遗传图谱。【方法】以谷子高146A和K103杂交自交F2分离群体为作图群体,以分布于谷子9条染色体上的81个SSR标记为主要参考标记,采用来自谷子、水稻、珍珠粟和高羊茅的SSR、STS、SNP、SV和ACGM标记共1 733个,在亲本间筛选多态性标记,并进一步在F2群体间进行验证,利用MAPMAKER VERSION 3.0软件进行连锁分析,采用MapDraw V2软件绘制遗传连锁图谱。【结果】构建了一张包含192个不同类型分子标记的谷子遗传图谱,新定位标记33个,其中32个来自谷子,另1个来自珍珠粟。遗传图谱包含9个连锁群,覆盖基因组全长2 082.5 cM,连锁群长度介于119.5-475.2 cM,平均长度231.39 cM,标记间平均距离10.85 cM,每个连锁群上的标记数介于10-37个。部分连锁群上标记存在偏分离现象,在定位的192个标记中,共有36个标记发生偏分离,占图谱总标记的18.75%,其中,在LG2、LG6和LG7上分别聚集分布了10、15和7个偏分离标记,出现了偏分离热点区域,而在LG1、LG4、LG5和LG8上仅有零星分布,LG3和LG9上则没有偏分离标记。对来自不同作物的分子标记在谷子上的可转移性分析发现,来自谷子的1 235个PCR标记中有205个标记在双亲及其F2群体间有多态性,多态率为16.60%,而来自珍珠粟、高羊茅和水稻的498个PCR标记,在该群体上仅发现1个多态性标记。【结论】利用不同物种中的分子标记构建了一张覆盖基因组长度为2 082.5 cM的谷子遗传连锁图谱,其分子标记主要来自于谷子。  相似文献   

8.
High sugar content of sorghum stalk is an important factor in the sorghum silage production. To identify the genomic regions controlling sugar content and to develop molecular markers linked to sugar content in sweet sorghum, we used an F2：3 segregating population consisting of 207 individuals derived from a cross between a high sugar content inbred line, Early Folger, and a normal inbred line, N32B, for genetic linkage mapping and quantitative trait locus （QTL） analysis. We constructed a genetic linkage map spanning 983.5 cM based on a total of 327 markers comprising 31 restriction fragment length polymorphism （RFLP） markers, 254 amplified fragment length polymorphism （AFLP） markers, and 42 simple sequence repeat （SSR） markers. In the 20 linkage groups detected, 98.2% of markers aligned to the 10 linkage groups of sorghum. Variations in sugar content at different growth stages and among internodes suggested that the sugar content of middle internodes is stable and suitable for measuring at early dough stage. The broad sense heritability （hB0 of sugar content was 0.64 and 0.62 estimated from the data of F3 families and each parent in 2003 and 2004. We identified one and two QTLs accounting for 22.2 to 25.0% of phenotypic variance using simple interval mapping method in 2003 and 2004, respectively. These two QTLs showed a negative additive effect, and over-dominance effect. A QTL on LG-D was detected in both two years. Above results will be help us to understand the genetic mechanism of sugar content in sorghum and the QTL detected in this study might be useful in the improvement of sugar content by marker-assisted selection.  相似文献   

9.
对火炬松×加勒比松杂种部分形质性状的QTL进行定位分析,为杂种育种和株型培育提供借鉴。以火炬松1.5代种子园优良单株T1为母本,加勒比松第一代种子园优良单株C1为父本,杂交产生F1代113个单株。使用JoinMap? 3.0作图软件构建火炬松母本和加勒比松父本的SSR 标记遗传图谱,并用MapQTL4.0?作图软件对形质性状的QTL进行定位。火炬松母本图谱总长度293.9 cM,标记间距范围为3.7~ 47.9 cM,标记平均间距15.5 cM。加勒比松父本图谱总长度245.5 cM,标记间距范围为6.8~ 49.8 cM,标记平均间距13.6 cM。检测到4个与形质性状有关的QTL位点,其中有2个QTL定位于火炬松染色体LG4上。4个形质性状的QTLs解释方差为8.6%～13.6%,可以利用这些QTLs进行特定株型的培育,如分枝角度的控制培育。  相似文献   

10.
基于四交群体的玉米叶夹角和叶向值QTL定位分析   总被引：1，自引：0，他引：1  
选育耐密紧凑株型是增加玉米单位面积产量的重要途径之一,而叶夹角和叶向值是衡量株型的重要参数。本研究选用叶夹角和叶向值存在差异的玉米自交系郑58、PH6WC、87-1和自330构建1个四交(郑58/豫87-1//PH6WC/自330)组合,以228个四交F1单株为作图群体,构建了1张含225个SSR位点,全长1 387.2cM的玉米分子标记遗传连锁图谱,标记间平均间距为6.19cM。基于四交群体应用区间作图法检测4个环境下的QTL,共检测到13个叶夹角相关QTL,分别位于第1、2、3、4、5、7和10染色体上,单个QTL可解释5.1%~20.0%的表型变异;检测到15个叶向值相关QTL,分别位于第1、2、4、5、7、8和9染色体上,单个QTL可解释5.4%~20.1%的表型变异。其中qLA-E2-2和qLA-E4-2落在同一标记区间umc1692-umc2297(bin 5.03),分别解释16.6%和13.2%的表型变异;qLO-E1-1、qLO-E3-2和qLA-E4-1落在同一标记区间umc1568-bnlg1953(bin1.02),分别解释10.1%、19.9%和12.3%的表型变异;qLO-E2-1和qLO-E3-1落在同一标记区间phi056-phi427913(bin 1.01),分别解释13.8%和10.0%的表型变异。这些多个环境共同检测到的QTL将为玉米耐密理想株型育种中叶夹角叶向值的分子标记辅助选择提供有益信息,加速耐密株型玉米品种的选育。  相似文献   

11.
玉米抗矮花叶病QTL的定位分析   总被引：1，自引：0，他引：1  
以黄早四（抗）和Mo 17（感）为亲本建立了239个RIL系的群体,利用该群体构建了包含101个SSR标记的遗传图谱,覆盖玉米基因组1422．7cM,标记间的平均距离为15.6cM。通过人工接种鉴定,评价了亲本及239个RIL系对MDMV病毒B株系的抗性反应。采用复合区间作图法对玉米矮花叶病抗性QTL进行了定位及其遗传效应分析,在第5,6染色体上各定位了控制发病率的1个微效QTL和一个主效QTL,分别与标记bnlg602,bnlg161连锁,其遗传效应能分别解释表型方差的2．3％和33．8％。在第6染色体上定位了控制病情指数的2个主效QTL,分别与标记bnlg161,y1 ssr连锁．其遗传效应分别能解释表型方差的56．5％和76．8％。  相似文献   

12.
The major incompatibility barriers to specific inbred lines and the long generation duration in Pyrus L. may hinder the Pyrus breeding process. A genetic linkage map provides the foundation for quantitative trait loci (QTL) mapping and molecular marker-assisted breeding. In this study, we constructed a genetic map with 145 F1 populations from a cross of two cultivars, Yali and Jingbaili, using AFLP and SSR markers. The map consisted of 18 linkage groups which included 402 genetic markers and covered 1395.9 cM, with an average genetic distance of 3.8 cM. The interval mapping was used to identify quantitative trait loci associated with four leaf agronomic traits in the F1 population. The results indicated that four QTLs were associated with leaf length, two QTLs with leaf width, two with leaf length/leaf width, and three with petiole length. The eleven QTLs were associated with 9.9%–48.5% of the phenotypic variation in different traits. It is considered that the map covers almost the whole genome, and molecular markers will be greatly helpful to the related breeding.  相似文献   

13.
基于90K芯片标记的小麦芒长QTL定位   总被引：1，自引：1，他引：0  
【目的】麦芒对小麦的抗逆性以及穗部光合等方面具有重要影响。研究旨在挖掘控制芒长的主效QTL及与其紧密连锁或共分离的分子标记,为全基因组分子标记辅助选择育种、近等基因系的构建、候选基因的筛选以及基因克隆提供依据。【方法】以小偃81/周8425B、小偃81/西农1376这2个组合的F9代RIL群体（分别含有102个和120个家系）为作图群体,利用覆盖小麦21条染色体组的90K标记构建2个遗传连锁图谱,于2016年10月至2017年6月将这2个F9群体衍生的F9﹕10群体分别种植在陕西杨凌、河南南阳和河南驻马店,小麦蜡熟期对芒长进行表型鉴定,用完备区间模型和多环境的联合分析对此性状进行QTL定位。【结果】构建了覆盖小麦21条染色体的2张遗传图谱,图谱长度分别为4 412.14和4 281.67 cM,平均遗传距离为分别为2.65和2.31 cM。2个连锁图谱的连锁标记数表明,90K标记在小麦基因组A、B和D间分布不均衡,但均表现为B基因组的标记数＞A基因组的标记数＞D基因组的标记数。对于2个连锁图谱的公共标记而言D基因组公共标记最少,从侧面反映出D基因组具有较高的保守性。2个RIL群体在陕西杨凌、河南南阳和河南驻马店3个环境下共检测到6个控制芒长的QTL。其中主效位点Qal5A-1在2个群体3种环境下都能被检测到,属于环境钝感QTL,表型变异贡献率变幅为46.01%-79.82%,对芒长具有强烈的抑制作用,加性效应来自短芒亲本小偃81,该主效QTL位点被定位在5A染色体末端,与分子标记RAC875_c8121_1147紧密连锁。另外Qal6B-1、Qal1B-1、Qal3B-1、Qal2D-1和Qal2D-2这5个QTL,分别被定位在6B、1B、3B、和2D染色体上,其表型变异的贡献率分别为1.39%、3.66%、3.93%、5.53%和3.51%,为微效QTL。小偃81/周8425B组合的RIL群体共检测的2个QTL,其中1个主效位点Qal5A-1和1个微效QTL位点Qal6B-1,2个QTL表型变异的贡献率总和为79.91%。小偃81/西农1376组合的RIL群体检测出5个QTL,1个主效位点Qal5A-1和4个微效位点Qal1B-1、Qal3B-1、Qal2D-1和Qal2D-2,5个QTL表型变异的贡献率总和为63.96%。多环境的联合分析得到了6个QTL,其互作效应的表型变异贡献率都远低于加性效应的表型变异贡献率,说明QTL与环境间的互作不是影响芒长的主要因素;加性效应值在不同的环境下近似相等,进一步表明这6个QTL在3个环境间有着稳定的遗传效应。【结论】2个群体检测到1个主效位点Qal5A-1,此位点能够稳定表达且与分子标记RAC875_c8121_1147紧密连锁,表型变异贡献率46.01%-79.82%,对芒长具有较强的抑制作用。  相似文献   

14.
Protein and starch are the most important traits in determining processing quality in wheat. In order to understand the genetic basis of the influence of Waxy protein (Wx) and high molecular weight gluten subunit (HMW-GS) on processing quality, 256 recombinant inbred lines (RILs) derived from the cross of waxy wheat Nuomai 1 and Gaocheng 8901 were used as mapping population. DArT (diversity arrays technology), SSR (simple sequence repeat), HMW-GS, and Wx markers were used to construct the molecular genetic linkage map. QTLs for mixing peak time (MPT), mixing peak value (MPV), mixing peak width (MPW), and mixing peak integral (MPI) of Mixograph parameters were evaluated in three different environments. The genetic map comprised 498 markers, including 479 DArT, 14 SSR, 2 HMW-GS, and 3 Wx protein markers, covering 4 229.7 cM with an average distance of 9.77 cM. These markers were identified on 21 chromosomes. Eighteen additive QTLs were detected in three different environments, which were distributed on chromosomes 1A, 1B, 1D, 4A, 6A, and 7D. QMPT-1D.1 and QMPT-1D.2 were close to the Glu-D1 marker accounting for 35.2, 22.22 and 36.57% of the phenotypic variance in three environments, respectively. QMPV-1D and QMPV-4A were detected in all environments, and QMPV-4A was the nearest to Wx-B1. One minor QTL, QMPI-1A, was detected under three environments with the genetic distances of 0.9 cM from the nearest marker Glu-A1, explaining from 5.31 to 6.67% of the phenotypic variance. Three pairs of epistatic QTLs were identified on chromosomes 2D and 4A. Therefore, this genetic map is very important and useful for quality trait related QTL mapping in wheat. In addition, the finding of several major QTLs, based on the genetic analyses, further suggested the importance of Glu-1 loci on dough mixing characteristics.  相似文献   

15.
Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.  相似文献   

16.
【目的】水稻栽培区土壤的盐、碱化日趋严重,植物体内Na+、K+浓度及Na+/K+是植物耐盐、碱性重要指标。在盐、碱胁迫条件下检测水稻苗期地上部和根部的Na+、K+浓度及Na+/K+的QTL位点,为水稻的耐盐、碱性遗传机制及分子标记辅助育种提供理论依据。【方法】以优质高产水稻品种东农425与耐盐、碱水稻品种长白10为亲本构建重组自交系(RIL)为作图群体,利用102对SSR标记构建遗传连锁图谱,该图谱覆盖水稻基因组约1 915.05 c M,标记间平均距离为18.77 c M;在140 mmol·L-1 Na Cl盐胁迫和0.15%Na2CO3碱胁迫处理条件下,对水稻苗期地上部和根部的Na+、K+浓度及Na+/K+等性状进行测定,利用SPSS v19.0对各性状进行相关分析,并采用QTL Ici Mapping v3.3的完备区间作图法(ICIM)进行QTL定位。【结果】盐、碱胁迫条件下,亲本及RIL群体地上部Na+、K+浓度均高于地下部Na+、K+浓度,各性状在RIL群体中基本符合正态分布,表现出典型的数量性状遗传特征,符合QTL定位要求。相关分析结果表明,盐、碱胁迫条件下,地上部Na+与K+及根部Na+与K+均呈极显著正相关,2种胁迫条件下的各性状相关性不显著。盐、碱胁迫条件下共检测到15个与Na+、K+浓度和Na+/K+相关的QTL,2种条件下所检测到的QTL位于不同染色体区域。在盐胁迫下共检测到5个QTL,包括1个与地上部K+浓度相关QTL,位于第8染色体的RM1308—RM281区间内,贡献率为6.83%;3个与根部Na+浓度相关QTL,位于第3和第8染色体上,其中q SRNC3-1贡献率最大,为16.41%;1个与根部K+浓度相关QTL,贡献率为3.52%;未检测到与地上部Na+浓度、Na+/K+及根部Na+/K+相关的QTL。在碱胁迫下共检测到10个QTL,包括1个与地上部Na+浓度相关的QTL,位于第2染色体的RM1347—RM48区间内,贡献率为14.41%;1个与地上部K+浓度相关QTL,位于第2染色体的RM1255—RM213区间内;3个与地上部Na+/K+相关QTL,分别位于第2、7、10染色体上,其中q ASNK2贡献率最大,为7.57%;1个与根部Na+浓度相关QTL,位于第3染色体的RM293—RM232区间内,贡献率为13.71%;2个与根部K+含量相关QTL,分别位于第1染色体的RM5—RM9和第2染色体的RM12865—RM12941区间内;2个与根部Na+/K+相关QTL,分别位于在第3和第4染色体上,其中q ARNK3贡献率较大,为10.48%。通过比较图谱发现,本研究中的大部分QTL与以往不同群体中影响耐盐、碱相关性状的QTL定位在同一或相邻的染色体区域,另外在碱胁迫下所检测到的q ASKC2和q ARKC2在前人研究中未见报道,可能存在新的耐碱性位点。【结论】在盐、碱胁迫条件下,Na+、K+的吸收和运输均是平行而独立的过程,且根部对Na+和K+的吸收与向地上部运输存在不同的遗传机制;盐、碱胁迫条件下,水稻Na+、K+浓度的遗传是相互独立的。  相似文献   

17.
温室黄瓜产量相关农艺性状QTLs的定位   总被引：7，自引：3，他引：4  
【目的】秋冬茬和冬春茬是目前中国日光温室黄瓜栽培的两种重要茬口,对两茬黄瓜产量相关性状的QTLs进行定位,为温室黄瓜产量分子标记辅助选择的研究提供理论依据。【方法】选用欧洲8号×秋棚自交系的113份黄瓜重组自交系(RILs)群体作为试验材料,并利用该群体已经构建的包含182个标记的分子连锁图谱对与产量相关的9个性状进行QTL分析。【结果】共检测到58个QTLs,其中与单株平均产量相关的QTL1个,定位于LG4连锁群上;控制黄瓜日增重量的QTL位点6个,分别位于LG2、LG3、LG6连锁群上;控制平均单瓜重的QTL位点5个,分别位于LG1和LG5连锁群上;控制坐瓜数的QTLs2个,位于LG2和LG4连锁群上;控制化瓜率的QTL1个,位于LG7连锁群上;控制第一雌花节位的QTLs28个,在1-8个连锁群上都有分布;控制总叶片数的QTLs8个,分别位于LG2、LG7和LG4连锁群上;控制叶面积的QTLs2个,分别位于LG1和LG3连锁群上。以上产量相关性状的QTLs仅在一个茬口中被检测到。控制雌花总数的QTLs5个,全部位于LG2连锁群上,其中ffa2a、ffa2b是两个茬口共有的QTLs,并且其遗传效应方向一致。研究还发现若干QTL富集区域和成束分布的QTLs。【结论】本项研究共检测到温室黄瓜与产量相关的9个性状的58个QTLs,其中ffa2a、ffa2b在两个栽培环境中表达稳定。  相似文献   

18.
【目的】豆象是危害豇豆最主要的仓储害虫。发掘豇豆抗豆象基因为抗性品种的选育,以及减少豆象对豇豆生产的危害奠定基础。【方法】以中豇1号（感）和Pant-lobia-1（抗）为亲本构建的包含282个株系的RIL群体为研究材料,利用人工接种法分别对282个株系接种绿豆象和四纹豆象,进行抗豆象表型鉴定,并利用两亲本对3 992个来源于绿豆、小豆和豇豆的SSR标记进行多态性筛选,然后利用筛选到的多态性标记对282个株系进行基因型分析,最后结合RIL群体各株系抗豆象表型鉴定数据和基因型分型数据,采用完备区间作图法（ICIM-ADD）进行抗豆象QTL定位分析,在此基础上构建遗传连锁图谱,并定位豇豆抗豆象基因。【结果】中豇1号和F1籽粒的被害率均为100%,Pant-lobia-1籽粒的被害率分别为22.5%和42.5%。推测Pant-lobia-1对绿豆象和四纹豆象的抗性均为隐性遗传;筛选到182个多态性标记,利用这些多态性标记构建了一个包含11个连锁群的豇豆遗传连锁图谱,图谱总长1 065.23 cM,相邻标记间平均遗传距离为5.85 cM;经2种豆象处理,分别在连锁群1和连锁群5上检测到2个稳定的QTL位点,暂定名为vubr1-1和vubr5-1,其中vubr1-1位于标记XD11-44和HAAS_VR_2274之间,标记间的遗传距离为7.6 cM,在2种豆象处理中分别可以解释表型变异的7.16%和6.92%;vubr5-1位于标记XD1-14和CP185之间,标记间的遗传距离为2.90 cM,在2种豆象处理中分别可以解释表型变异的6.96%和6.37%。【结论】构建了一个包含11个连锁群、182个多态性标记的豇豆遗传连锁图谱,检测到2个与抗豆象相关的QTL位点。  相似文献   

19.
油菜籽含油量和蛋白质含量的种子胚与母体植株QTL定位   总被引：1，自引：0，他引：1  
【目的】利用甘蓝型油菜TN DH群体分别与双亲Tapidor和Ningyou7回交构建的BC1F1 1和BC1F1 2两个群体,分析油菜籽含油量和蛋白质含量的种子胚和母体植株两套不同核基因组的QTL及其遗传效应,以明确QTL在不同遗传体系中的分布状况以及连锁的分子标记,研究环境互作效应对不同遗传体系QTL定位的影响,探讨相应品质性状分子标记辅助选择的最优策略和方法。【方法】按照常规田间试验方法种植202个TN DH群体材料与双亲,采用2年、2次重复、随机区组试验设计,开花时通过双向回交构建BC1F1 1和BC1F1 2两个群体,收获双亲和回交群体的种子。利用可分析含油量和蛋白质含量的近红外分析模型和方法测定油菜籽含油量和蛋白质含量。结合甘蓝型油菜分子标记连锁遗传图谱以及新创建的双子叶作物种子品质性状两套遗传体系的QTL定位方法和作图软件,对不同年份BC1F1 1和BC1F1 2油菜籽含油量和蛋白质含量进行QTL定位分析。【结果】共检测到7个与油菜籽含油量和蛋白质含量相关的QTL,分布在A1、A4、A6、A7、C2和C5连锁群上,其中,4个与含油量相关的QTL和3个控制蛋白质含量的QTL对表型的总贡献率分别为49.1%和59.6%。检测到的QTL均具有极显著的胚加性主效应和母体加性主效应,其中4个QTL具有显著或极显著的胚显性主效应、2个与含油量相关的QTL具有极显著的环境互作效应。qOC-6-3和qPC-4-1作为控制含油量和蛋白质含量的重要QTL,分别能解释36.3%和37.9%的表型变异;而qOC-4-2和qPC-4-1均被定位在甘蓝型油菜A4连锁群相同的位点上,位于分子标记HS-K02-2和HBR094之间,QTL峰值位置为18.5 cM,置信区间为17.5-19.4 cM。【结论】甘蓝型油菜籽含油量和蛋白质含量的表现会同时受到种子胚和母体植株两套不同遗传体系核基因组QTL表达效应的影响,其中环境互作效应对含油量表现的作用更为明显,而控制蛋白质含量表现的QTL在不同环境条件下的表达较为稳定。在A6和A4连锁群上检测到的qOC-6-3和qPC-4-1是2个控制含油量和蛋白质含量的主效QTL,同时2个控制蛋白质含量的QTL尚未见报道。  相似文献   

20.
【目的】构建裸燕麦分子遗传图谱,发掘燕麦β-葡聚糖基因紧密连锁的分子标记,为高β-葡聚糖含量燕麦种质资源的利用及裸燕麦分子标记辅助育种提供理论和实践依据。【方法】以高β-葡聚糖地方品种夏莜麦为父本,育成品种赤38莜麦为母本构建的包含215个F2:3家系为图谱构建群体,利用SSR分子标记进行遗传分析,构建分子遗传图谱。通过美国谷类化学会（AACC）发表的标准葡聚糖含量测定方法（AACC Method 32-23）测定各家系的β-葡聚糖含量,利用复合区间作图法进行燕麦β-葡聚糖含量性状进行遗传定位与分析。【结果】利用筛选出的231对SSR引物在F2 后代群体上进行检测,共得到261个多态性标记位点,利用JoinMap 4.0软件对上述获得多态性分子标记进行遗传连锁分析,在LOD≥5.0情况下,构建遗传图谱,得到包含26个连锁群、182个标记位点的遗传图谱,覆盖基因组1 869.7 cM,标记间平均距离为10.6 cM,每个连锁群上的标记数在2-14个之间,连锁群长度在10.6-235.1 cM。对亲本及后代群体β-葡聚糖含量的测定结果表明,β-葡聚糖含量在后代群体中表现出明显的分离,且呈现为连续变异,变异系数为18.72%,说明β-葡聚糖含量性状是受多基因控制的数量性状,群体符合QTL定位的要求。利用QTL分析软件WinQTLCart 2.5对SSR数据进行分析,采用复合区间作图法（composite interval mapping,CIM）对全基因组进行QTL扫描,以LOD值5作为阈值对β-葡聚糖含量可能存在的QTL进行定位和效应估计,检测到4个与β-葡聚糖含量相关的QTL位点,其中qBG-1位于连锁群LG20上,与最近的标记AM591的距离10.0 cM。加性效应值为0.21,可以解释的表型变异为10.9%;qBG-2和qBG-3位于连锁群LG23上,其中qBG-2与最近的标记AM1823的距离4.6 cM,qBG-3与最近的标记AM641的距离1.9 cM,加性效应值分别为-0.23和-0.22,可以解释的表型变异分别为3.2%和2.7%;qBG -4位于连锁群LG25上,与最近的标记AM302的距离6.8 cM,加性效应为0.84,可以解释的表型变异为27.6%,其中存在的2个主效QTL qBG-1和qBG -4,都来自于高β-葡聚糖含量的父本夏莜麦。【结论】构建了大粒裸燕麦SSR分子标记连锁群图谱,并定位了4个控制β-葡聚糖含量的QTL位点。  相似文献   

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