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1.
为研究手掌参多糖对电离辐射损伤小鼠造血和抗氧化功能的治疗作用,本试验选用48只健康雌性昆明小白鼠,随机分为8组,分别为空白组,辐射组,手掌参多糖低、中、高给药组(150、300、600 mg·kg-1 组)和辐射低、中、高给药组;辐射组和辐射给药组分别用60Co-γ射线进行辐射,辐射剂量5.0 Gy(剂量率0.8 Gy·min-1)。分别在照射后 30 min内灌胃相应剂量的手掌参多糖和生理盐水(空白组、辐射组),连续5 d。末次灌胃后48 h内测定所有小鼠外周血细胞计数、脾脏指数、肝脏超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量、骨髓DNA含量、骨髓嗜多染红细胞微核 (MN) 数目及脾脏T淋巴细胞亚群。结果表明,与空白组相比,给药组小鼠抗氧化能力和造血系统得到不同程度的改善,辐射组外周血细胞计数、脾脏指数、骨髓DNA含量、肝脏抗氧化能力及T淋巴细胞亚群极显著降低,MDA含量与MN数目显著升高; 与辐射组相比,辐射给药组小鼠脾脏指数和T-AOC、SOD活性极显著升高,DNA含量、外周血细胞计数也有所升高,CD3+、CD4+、CD8+ T淋巴细胞比率升高,MDA含量、MN数目显著降低。上述试验结果表明,手掌参多糖对60Co-γ射线照射造成的小鼠造血、抗氧化功能损伤具有治疗作用。本研究为手掌参多糖对辐射损伤的治疗提供了技术参考。 相似文献
2.
为了明确电子束辐照对人参抗肿瘤作用的影响,以长白山林下参为研究对象,电子束辐照后测定人参皂苷的含量,Hek-293、HCT-116、Hela、HepG2细胞的抑制情况以及荷瘤小鼠的治疗情况,并测定荷瘤小鼠血清MDA含量、T-SOD活性和T-AOC水平。结果表明,辐照能够提高人参中皂苷的含量,其中人参皂苷Rg3含量变化明显。体外试验中,30 kGy辐照组人参提取物对HCT-116、Hela、Hep G2细胞的最高抑制率分别达到75.63%、85.33%、84.87%,均显著高于未辐照人参组;体内试验中,辐照人参组瘤重低于阴性对照组,同时能够提高荷瘤小鼠体内抗氧化能力,从而提高小鼠机体抗肿瘤能力。本试验结果为人参抗肿瘤作用的研究提供了新的思路。 相似文献
3.
为探究雅安藏茶茶多糖对~(60)Co-γ射线辐射损伤小鼠抗辐射作用的影响,从雅安藏茶中提取茶多糖,用不同浓度(50、100、200 mg·kg-1·d-1)的茶多糖连续15 d灌胃小鼠,在第6天采用5Gy的~(60)Co-γ射线一次性全身辐照各组小鼠,灌胃第16天测定各组小鼠外周血细胞、肝脏组织总抗氧化能力(T-AOC)、总超强氧化物歧化酶(T-SOD)活性和总蛋白(TP)含量等指标。结果表明,与单纯辐照组相比,雅安藏茶茶多糖能显著提高辐照损伤小鼠的外周血细胞数量,极显著提高肝脏组织T-AOC、T-SOD活性和股骨骨髓DNA含量,极显著降低肝脏MDA含量,缓解了免疫器官胸腺的萎缩;且随着茶多糖浓度的增加,其抗辐射作用效果增强。综上,雅安藏茶茶多糖对~(60)Co-γ射线辐照损伤小鼠抗氧化功能和造血功能具有较强的防护作用,且具有一定的剂量效应。本研究结果为进一步深入探究茶多糖防辐射作用机理提供了新参考,也为雅安藏茶辐照保健功效研究提供了一定的理论依据。 相似文献
4.
为探讨金蝉花多糖对环磷酰胺(CTX)致免疫抑制小鼠的免疫调节作用,将70只试验小鼠随机分为7组,除正常组外,其他6组腹腔注射CTX以建立免疫低下模型,其中,高、中、低剂量组灌胃不同剂量的金蝉花多糖溶液30 d,正常对照组、CTX模型组给予等体积生理盐水,茯苓多糖组、盐酸左旋咪唑组分别给予等体积茯苓多糖溶液和盐酸左旋咪唑溶液,分别测定各组的小鼠体重增量、免疫器官指数、迟发型超敏反应、血清溶血素水平、IFN-γ、IL-2、IgG含量等指标。结果表明,与CTX模型组相比,金蝉花多糖中、高剂量组能显著提高小鼠脾脏指数、胸腺指数、血清溶血素水平(P0.05);各剂量组均能显著影响小鼠迟发型超敏反应(P0.05),均显著提高了IFN-γ、IL-2、IgG含量(P0.05),说明金蝉花多糖对小鼠免疫功能具有一定的调节作用。本研究结果为进一步探究金蝉花多糖的免疫作用机制提供了参考,有利于金蝉花多糖在保健功效方面的应用研究。 相似文献
5.
研究了姜辣素对60Coγ射线损伤小鼠造血和抗氧化功能的治疗作用。18只雌性健康昆明小鼠被随机分为3组,每组6只,分别为对照组、照射组、照射给药组。对照组不照射,连续灌胃5d蒸馏水;照射组和照射给药组分别用3Gy60Coγ射线进行照射,并分别在照射后30min内灌胃蒸馏水和姜辣素,连续5d。末次灌胃后48h内测定所有小鼠血液中白细胞(WBC)和红细胞(RBC)数量、脏器指数、肝脏超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量及骨髓嗜多染红细胞微核(MN)数目。与对照组相比,照射组脾脏指数、WBC极显著降低(P<0.01),肝脏MDA含量、骨髓MN数目极显著升高(P<0.01);与照射组相比,照射给药组脾脏指数、T-AOC、SOD活性极显著升高(P<0.01),肝脏指数、WBC、RBC含量也有所升高,MDA含量、MN数目极显著降低(P<0.01)。结果表明,姜辣素对60Coγ射线照射造成的小鼠造血、抗氧化功能损伤具有治疗作用。 相似文献
6.
本文建立高效的亮菌多糖分离纯化工艺,并研究其体内抗肿瘤活性。以市售亮菌粗粉为原料,采用水提醇沉、Sevag法脱蛋白、DEAE纤维素离子交换层析、SephadexG-100凝胶过滤层析等方法得到亮菌多糖ATPSⅡ和ATPSⅡ-2;建立小鼠S180实体瘤模型,以生理盐水和环磷酰胺为阴性和阳性对照,观察ATPSⅡ和ATPSⅡ-2在不同剂量（10mg／kg40mg／kg80mg／kg）给药10d后,对小鼠肿瘤抑制的作用。结果表明：亮菌粗粉中多糖含量为22．7g／100g;紫外光谱分析显示纯化得到的ATPSⅡ纯度接近100％;ATPSⅡ和ATPSⅡ-2对肿瘤的抑瘤率随着多糖浓度的增大而增大,其高剂量组对S180的抑瘤率分别为46．7％和51．7％,与阳性对照组相比,亮菌精制多糖ATPSⅡ和ATPSⅡ-2高剂量组抗s180效果接近于临床化疗药环磷酰胺,具有显著的抗肿瘤活性。 相似文献
7.
Z0206细菌富硒多糖对小鼠的免疫调节作用研究 总被引：1，自引：0，他引：1
本试验通过建立环磷酰胺（CP）诱导的免疫抑制小鼠模型,研究了Z0206细菌富硒多糖的免疫调节功能。试验分为四个处理：Ⅰ组为对照组;Ⅱ组为CP组;Ⅲ组为细菌富硒多糖组;Ⅳ组为细菌富硒多糖预防组。结果表明,与对照组相比,细菌富硒多糖组小鼠免疫器官指数、NK细胞活性、血液溶血素水平（HC50）和脾细胞抗体形成数（PFC）均显著提高（p<0.05）,T细胞亚群增殖有所提高,但差异不显著（p>0.05）。与CP组相比,细菌富硒多糖预防组能使CP诱导的免疫抑制得到修复,改善试验小鼠免疫器官指数、T淋巴细胞和自然杀伤细胞（NK）活性、血液溶血素水平和脾细胞抗体形成数,差异显著（p<0.05）。提示：细菌富硒多糖能增强小鼠的免疫功能,对CP诱导的免疫抑制具有修复作用。 相似文献
8.
为研究牦牛血低聚肽对缺氧所致H9c2心肌细胞损伤以及对运动后小鼠抗疲劳能力的影响,本研究通过建立H9c2 细胞缺氧损伤模型以及小鼠负重游泳试验,研究其抗缺氧能力以及抗疲劳能力。结果表明,与正常对照组相比,缺氧组的H9c2细胞存活率极显著降低(P<0.01),表明缺氧对H9c2心肌细胞有一定损伤;与缺氧组相比,低聚肽干预组的细胞存活率增加且存在剂量依赖性;与常用抗缺氧药物红景天苷相比,牦牛血低聚肽高剂量组细胞存活率为75.1%,而红景天苷组为88.0%。牦牛血低聚肽及其复合肽均能延长小鼠负重游泳时间,提高运动后小鼠肝脏肝糖元(HG)含量,降低运动后小鼠血清乳酸(BLA)和血清尿素氮(BUN)含量。综上,牦牛血低聚肽对缺氧介导的心肌细胞损伤具有一定的保护作用,能提高小鼠的抗疲劳能力,且牦牛血低聚肽与大豆低聚肽按照 5∶5 比例复配后的抗疲劳能力优于单一使用牦牛血低聚肽。本研究结果为耐缺氧活性因子的研究提供了一条新途径。 相似文献
9.
为探讨人红白血病HEL细胞经羊栖菜硫酸多糖(SFPS Ⅱ)处理后基因表达谱的变化,本研究用SFPS Ⅱ作用HEL细胞24 h,应用转录组技术筛选差异表达基因,分析差异基因富集的GO功能和KEGG信号通路,并通过实时荧光定量PCR(RT-qPCR)对部分差异表达基因进行验证。结果表明,SFPS Ⅱ处理HEL细胞后,HEL细胞活力降低,呈浓度依赖性,但对正常人胚肺成纤维细胞MRC-5几乎无毒性。与对照组相比,共有1 248个基因差异表达,其中219个上调,1 029个下调。GO分析显示,差异表达基因主要改变DNA构象、硫酸盐转运及对肿瘤坏死因子和外在凋亡信号反应等生物学功能。KEGG信号通路分析显示,显著富集的通路与Rap 1信号通路等癌症和免疫密切相关。试验随机选择16个显著差异基因,并通过RT-qPCR进行确认,发现SFPS Ⅱ诱导HEL细胞后,导致表达差异基因与肿瘤生物学进程和通路显著相关。本研究结果为揭示羊栖菜硫酸多糖抗肿瘤机制的提供了理论依据。 相似文献
10.
在结核分枝杆菌(Mycobacterium tuberculosis,MTB)与宿主巨噬细胞的相互作用中,巨噬细胞的凋亡对抵御及杀灭MTB起着重要的作用。为了探讨MTB的BCG和H37Ra两个弱毒株对巨噬细胞凋亡的影响及其相关机制,本研究比较了体外培养的BCG和H37Ra两种菌株感染小鼠(Mus musculus)巨噬细胞后对细胞凋亡的影响,并检测了肿瘤坏死因子-alpha(tumor necrosis factor-α,TNF-α)、凋亡相关蛋白半胱氨酸蛋白酶-3(Caspase-3)和B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)的表达情况。结果发现,与对照组相比,BCG和H37Ra感染后显著提高了巨噬细胞的凋亡率(P<0.05),TNF-α和Caspase-3的表达及活性也显著增加(P<0.01或P<0.05),同时极显著抑制了Bcl-2的表达(P<0.01)。但BCG感染组细胞凋亡率极显著高于H37Ra组(P<0.01),BCG感染组Caspase-3的表达和活性极显著高于H37Ra组(P<0.01),而Bcl-2和TNF-α的表达在BCG和H37Ra感染组间差异不显著(P>0.05)。结果提示,BCG和H37Ra两个弱毒株感染后对巨噬细胞凋亡的影响不同,可能与Caspase-3的表达相关,而与Bcl-2和TNF-α的表达无关。本研究为深入研究MTB毒力与巨噬细胞凋亡的相互作用机制提供了基础资料。 相似文献
11.
为探究番石榴结合态多酚对小鼠肠道菌群的影响,本试验将CD-1小鼠分为空白组、高浓度组(500 mg·kg-1)和低浓度组(100 mg·kg-1)3组,在灌胃5周后,收集小鼠的无菌粪便,扩增肠道菌群16S rRNA基因高变域V3-V4,通过16S rRNA基因扩增子测序技术检测小鼠肠道菌群的α多样性、β多样性,以及门、属水平的物种组成。结果表明,与空白组相比,低浓度组中菌群的均匀性和丰富度显著提高(P<0.05);基于门水平分析,厚壁菌门、拟杆菌门和疣微菌门3个菌门的相对丰度值较大;在属水平中,高低浓度组均能降低拟杆菌属相对丰度,低浓度组能提高乳杆菌相对丰度,并且高浓度组中艾克曼菌属的相对丰度大幅提高。综上所述,番石榴结合态多酚能提高小鼠肠道菌群的均匀性和丰富度,且对菌群结构具有调节作用,既能提高益生菌的相对丰度,也能降低有害菌的相对丰度。本研究结果可为番石榴结合态多酚的活性研究和实际应用提供理论参考。 相似文献
12.
Two-year toxicity and carcinogenicity study of methyleugenol in F344/N rats and B6C3F(1) mice 总被引：2，自引：0，他引：2
Johnson JD Ryan MJ Toft JD II Graves SW Hejtmancik MR Cunningham ML Herbert R Abdo KM 《Journal of agricultural and food chemistry》2000,48(8):3620-3632
Methyleugenol (MEG) was tested for toxicity/carcinogenicity in a 2-yr carcinogenesis bioassay because of its widespread use in a variety of foods, beverages, and cosmetics as well as its structural resemblance to the known carcinogen safrole. F344/N rats and B6C3F(1) mice (50 animals/sex/dose group) were given MEG suspended in 0.5% methylcellulose by gavage at doses of 37, 75, or 150 mg/kg/day for 2 yr. Control groups (60 rats/sex and 50 mice/sex) received only the vehicle. A stop-exposure group of 60 rats/sex received 300 mg/kg/day by gavage for 53 weeks followed by the vehicle only for the remaining 52 weeks of the study. A special study group (10 animals/sex/species/dose group) were used for toxicokinetic studies. All male rats given 150 and 300 mg/kg/day died before the end of the study; survival of female rats given 150 mg/kg/day and all treated female mice was decreased. Mean body weights of treated male and female rats and mice were decreased when compared to control. Area under the curve results indicated that greater than dose proportional increases in plasma MEG occurred for male 150 and 300 mg/kg/day group rats (6 and 12 month) and male 150 mg/kg/day mice (12 month). Target organs included the liver, glandular stomach, forestomach (female rats) and kidney, mammary gland, and subcutaneous tissue (male rats). Liver neoplasms occurred in all dose groups of rats and mice and included hepatoadenoma, hepatocarcinoma, hepatocholangioma (rats only), hepatocholangiocarcinoma, and hepatoblastoma (mice only). Nonneoplastic liver lesions included eosinophilic and mixed cell foci (rats only), hypertrophy, oval cell hyperplasia, cystic degeneration (rats only), and bile duct hyperplasia. Mice also exhibited necrosis, hematopoietic cell proliferation, and hemosiderin pigmentation. Glandular stomach lesions in rats and mice included benign and malignant neuroendocrine tumors, neuroendocrine cell hyperplasia, and atrophy and in mice included glandular ectasia/chronic active inflammation. In female rats, the forestomach showed a positive trend in the incidences of squamous cell papilloma or carcinoma (combined). Male rats also exhibited kidney (renal tubule hyperplasia, nephropathy, and adenomacarcinoma), mammary gland (fibroadenoma), and subcutaneous tissue (fibroma, fibrosarcoma) lesions. Male rats also exhibited malignant mesotheliomas and splenic fibrosis. These data demonstrate that MEG is a multisite, multispecies carcinogen. 相似文献
13.
In a previous study, a flavonoid-rich extract of Hypericum perforatum L. (FEHP) was prepared and its antioxidant activity was determined by a series of models in vitro. In this study, the hypocholesterolemic effects of FEHP in rats fed a cholesterol-rich diet were tested. Forty Wistar rats fed a standard laboratory diet or a cholesterol-rich diet for 16 weeks were used. The serum lipid levels, as well as malondialdehyde (MDA) and activity of superoxide dismutase (SOD) and catalase (CAT) in serum and liver, were examined. Cholesterol-rich diet induced hypercholesterolemia was manifested in the elevation of serum lipid levels such as total cholesterol (TC), total triglycerides (TG), and low density lipoprotein cholesterol (LDL-C). Administration of middle-dose (75 mg/kg of BW/day) and high-dose (150 mg/kg of BW/day) FEHP significantly lowered the serum levels of TC, TG, and LDL-C, while increasing the serum level of high density lipoprotein cholesterol (HDL-C). Also, the content of MDA in serum and liver decreased significantly after oral administration of FEHP compared with those of rats fed a cholesterol-rich diet. In addition, FEHP increased the activity of SOD in serum and liver, but the activity of CAT was significantly elevated only in liver. These results suggested that the hypocholesterolemic effects of FEHP might be due to its abilities to lower serum TC, TG, and LDL-C levels as well as to slow the lipid peroxidation process and to enhance the antioxidant enzyme activity. 相似文献
14.
Mushroom dietary fiber or nonstarch polysaccharides (NSPs) that were soluble in hot alkali and belonged to the beta-glucan type were isolated from the sclerotia of an edible mushroom, Pleurotus tuber-regium. The mushroom NSPs were further separated into a number of fractions [hot alkali extracts (HAEs)] with weight-average molecular weights (M(w)) ranging from 1 x 10(4) to 42.2 x 10(4). The HAE fractions [with M(w) of (5.8-17.1) x 10(4)] administered intraperitoneally at a dose of 20 mg/kg of body weight to BALB/c mice implanted with solid tumor Sarcoma 180 were found to be effective in inhibiting tumor proliferation with an inhibition ratio of > or =50%. In vitro experiments using human tumor cell lines HL-60 and HepG2 had shown that HAE fractions with M(w) of (5.8-42.2) x 10(4) also had antiproliferative activity at three different concentrations (50, 100, and 200 microg/mL) toward the tumor cell lines tested. All HAE fractions did not inhibit the growth of a normal kidney cell line (Vero) from monkey. It is therefore postulated that the antitumoral effect of NSPs from the sclerotia of P. tuber-regium is probably host-mediated and cytocidal. 相似文献
15.
He RR Wang M Wang CZ Chen BT Lu CN Yao XS Chen JX Kurihara H 《Journal of agricultural and food chemistry》2011,59(8):3730-3737
This study was conducted to investigate the effects of apple polyphenol extract (APE) against influenza virus in mice loaded with restraint stress. The high-performance liquid chromatography (HPLC) fingerprint of APE was recorded, and the percentage composition of polyphenols was determined as 81.7%. Our results showed that restraint stress significantly promoted the mortality and duration of complications of mice infected with the H1N1 virus. However, oral administration of APE (100 and 200 mg/kg) improved the survival rates and prolonged living time of stressed mice infected with influenza virus in a dose-dependent manner. APE was further found to significantly improve the number of immunocytes, ratio of CD4 helper cells, secretion of IL-2, and capabilities of natural killer (NK) cytotoxicity (LU10/spleen) in spleens of restraint-stressed mice. In addition, APE also significantly decreased the level of lipid peroxidation and increased oxygen radical absorbance capacity (ORAC) in splenocytes. These results indicated that the protective effects of APE on mice infected with influenza virus were related to the alleviation of stress-induced impairment of immune functions and its antioxidant property might contribute to the immune recovery. 相似文献
16.
不同氮、磷、钾、钙水平对香水百合切花品质效应的研究 总被引：1，自引：0，他引：1
以盆栽香水百合为试验材料,用氮、磷、钾、钙四因素四水平L16(44)正交试验设计方法,测定了百合切花形态、瓶插寿命、花瓣中的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、可溶性糖、可溶性蛋白质含量等指标,以期探讨配比施肥对香水百合切花品质的影响。结果表明,以施用氮肥300 mg/kg、磷肥100 mg/kg、钾肥150 mg/kg、钙肥120 mg/kg配比处理最佳,提高了百合的株高、花径叶面积,延长了切花瓶插寿命,维持了花瓣中SOD活性在较高水平,还减缓了瓶插期间花瓣中可溶性糖、可溶性蛋白质的下降。在瓶插3个时期内切花SOD活性、可溶性糖、可溶性蛋白质含量均与氮、磷、钙营养呈正相关,而与钾营养呈负相关。 相似文献