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猪睾丸中脂联素受体与LHR、CYP11A1、StAR基因表达的发育变化及其相关性研究 总被引：1，自引：0，他引：1
旨在了解皖南花猪睾丸组织脂联素受体mRNA与睾酮合成相关因子mRNA表达的发育性变化及其相关性,探讨脂联素与睾酮生成的关系.本研究用荧光实时定量PCR法检测0、30、45、90和180 d等不同日龄(各日龄样本数5头)皖南花猪睾丸组织脂联素受体(AdpR1、AdpR2)和LHR、CYP11A1、StAR mRNA的表达水平.结果,AdpR1、CYP11 A1和StAR mRNA的表达有极显著的发育性变化(P＜0.01),AdpR1 mRNA的表达先增加后降低,45 d达到最大值;CYP11A1和StAR mRNA的表达先升高后降低再升高,45 d达到最大表达量.LHR mRNA的表达也有显著的发育性变化(P＜0.05),整体表现为先升高后降低.AdpR1与CYP11A1 mRNA的表达量显著相关(r=0.587,P＜0.05);LHR与CYP11A1、StAR mRNA的表达量分别显著相关(r=0.528,P＜0.05;r=0.552,P＜0.05);CYP11 A1与StAR mRNA表达量极显著相关(r=0.709,P＜0.01).AdpR1mRNA在睾丸组织的高表达提示脂联素可能通过AdpR1介导对睾丸的调节作用,其发育性变化和CYP11A1 mRNA呈正相关,提示脂联素对睾酮的生成有一定的促进作用. 相似文献
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脂联素研究进展及其在畜禽生产中的应用前景 总被引：1，自引：0，他引：1
脂联素(Adiponectin)是主要由脂肪细胞分泌的细胞因子,通过受体(AdipoR1或Adi- poR2)作用于相关组织,激活过氧化物酶体增殖物激活受体α(PPARα)和AMP激酶(AMPK)信号转导通路,发挥调节胰岛素敏感性、维持机体能量平衡、调控体脂沉积等功效。 相似文献
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脂联素（AdipoQ）是一种主要由脂肪组织分泌的内源性细胞因子,在调节脂类代谢中发挥重要作用。AdipoQ首先与其受体[脂联素受体1(AdipoRl)和脂联素受体2(AdipoR2)]位于膜外的C端结合,再通过AdipoQ受体N端与信号接头蛋白结合,进而激活下游腺苷酸活化蛋白激酶（AMPK）与过氧化物酶体增殖物激活受体α(PPARα)等多条信号通路,促进脂肪酸氧化,抑制脂质合成,从而调节脂类代谢。本文就AdipoQ及其受体的结构、信号接头蛋白和AdipoQ对动物脂质代谢的调节机制进行综述。 相似文献
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脂联素及其受体对脂代谢信号转导通路的调控机制 总被引：1，自引：0，他引：1
脂联素(Adipo Q)是一种由脂肪组织分泌的细胞因子,在调节畜禽的脂代谢等方面具有重要作用。Adipo Q主要通过与脂联素受体1(Adipo R1)和脂联素受体2(Adipo R2)2种受体结合来调控腺苷酸活化蛋白激酶α(AMPKα)、p38丝裂原激活蛋白激酶(p38MARK)和过氧化物酶体增殖物激活受体α(PPARα)等信号转导通路,参与机体内的脂代谢途径。目前对Adipo Q介导的脂代谢信号转导通路的研究已有了一定进展。本文就Adipo Q及其受体的结构,以及Adipo Q及其受体对脂代谢的调控机制进行了综述。 相似文献
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脂联素是一种由脂肪组织分泌的具有多种生物学功能的特殊蛋白,在增强脂肪酸氧化、抗炎症反应、抗糖尿病等方面起重要作用。脂联素通过AdipoR1和AdipoR2这两种受体的介导经过AMPK、PPAR、p38MAPK等信号通路来发挥生物学作用。脂联素及其受体AdipoR1和AdipoR2能在多种组织器官中表达,AdipoR1主要在肌肉组织中表达,AdipoR2则高表达于肝脏组织。此外,脂联素及其受体还能在下丘脑、垂体、子宫、胚胎等多种生殖腺和生殖组织中表达,说明脂联素在调控动物生殖及胚胎生长发育方面起重要作用。 相似文献
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取单层生长状态良好的犊牛脂肪细胞,分别添加0、2.5、5、15、30、50、100 μg/mL外源牛重组脂联素（adiponectin,ADPN）,培养12 h后提取总RNA,采用荧光定量PCR方法检测外源脂联素对脂肪细胞ADPN mRNA和HSL mRNA表达水平的影响。结果显示,随着ADPN添加量的增多,ADPN mRNA相对表达量呈下降趋势;添加ADPN 15 μg/mL时,HSL mRNA的表达量达到峰值,之后呈下降趋势。结果表明体外培养的脂肪细胞中ADPN mRNA表达量受自身的负调节,而添加适量的ADPN可刺激脂肪细胞HSL mRNA的表达。 相似文献
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体外原代培养犊牛肝细胞,添加0、16、64和128μg/L脂联素（adiponectin,ADPN）,分别培养4、8h后,提取细胞总RNA。应用实时荧光定量PCR方法,检测脂氧化关键酶脂酰辅酶A氧化酶（ACO）、肝脏脂肪酸结合蛋白（L-FABP）、肉碱脂酰转移酶-Ⅰ（CPTⅠ）、肉碱脂酰转移酶-Ⅱ（CPTⅡ）mRNA表达变化。结果显示,在ADPN作用4h后,ACO、L-FABP、CPTⅠ、CPTⅡ基因mRNA水平均随ADPN浓度的增加而增加,且均显著高于对照组,呈剂量依赖关系;作用8h时,ACO表达也增加,但差异不显著,L-FABP在中高剂量组显著高于对照组,CPTⅠ和CPTⅡ在各ADPN处理组均显著高于对照组。结果表明,ADPN可显著促进脂氧化关键酶的表达,增强肝脏脂氧化作用,减少肝脂储存。 相似文献
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旨在探究不同发育阶段湖羊生殖器官中脂联素受体(adiponectin receptor,AdpR)及睾酮(testosterone,T)分泌相关基因mRNA的表达变化及相关性分析。选取3、9、24月龄(M)湖羊各5只,颈静脉采血并收集血清,屠宰后,采集下丘脑、垂体、睾丸、附睾头、体、尾组织;ELISA技术检测各月龄湖羊血清中脂联素(adiponectin,Adp)、T、促性腺激素释放激素(gonadotropin releasing hormone,GnRH)、促性腺激素抑制激素(gonadotropin inhibitory hormone,GnIH)浓度;qRT-PCT技术检测不同发育阶段湖羊下丘脑-垂体-性腺轴(hypothalamus-pituitary-gonad axis,HPG)中AdpR1和AdpR2及T分泌关键基因mRNA表达规律;免疫组化技术对AdpR1在湖羊睾丸和附睾中进行定位分析。结果表明:不同发育阶段湖羊血清中Adp、GnRH、GnIH浓度呈现不同的变化趋势,其中Adp含量在性成熟后(9和24月龄)显著高于性成熟前(3月龄)(P0.05)。AdpR1mRNA表达量在各个月龄睾丸和附睾尾中均显著高于AdpR2(P0.05),其中AdpR1mRNA表达量在二者中呈上升趋势,且差异显著(P0.05),而在附睾头、体差异不显著(P0.05)。AdpR1存在于睾丸间质细胞、生精细胞、曲精细管管壁肌样细胞、精子细胞以及附睾头、体、尾的上皮细胞。血清中Adp与T含量呈显著正相关(P0.05);Adp含量与GnIH负相关以及与GnRH正相关,但差异不显著(P0.05);AdpR1mRNA表达水平与StAR、3β-HSD mRNA表达水平呈显著正相关(P0.05)。综上表明,在湖羊生殖器官发育过程中,Adp通过与其受体AdpR1结合,促进T的产生,进而影响湖羊睾丸发育。 相似文献
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摘要：为探讨皖南花猪骨骼肌组织中脂联素受体（AdpRl、AdpR2）和不同类型肌球蛋白重链（MyHC）mRNA的发育性变化及性别差异,选择0（出生当天）、30、45、90、180日龄的皖南花猪公母各5头,以B～Actin为内标,采用△△Ct相对定量实时荧光PCR方法对背最长肌和半腱肌中AdpRl、AdpR2、MyHCI、MyHC2a、MyHC2b和My-HC2xmRNA进行定量分析。结果显示,背最长肌和半腱肌AdpRl、AdpR2、MyHCl、MyHC2a、MyHC2b和My—HC2XmRNA的表达都有显著或极显著的发育变化规律（P〈0．05或P〈0．01）。总体上AdpRl、AdpR2、My—HC2a、MyHC2b和MyHC2XmRNA在背最长肌显著或极显著高于半腱肌（P〈0．05或P〈0．01）;MyHClmRNA在背最长肌极显著低于半腱肌（P〈0．01）。半腱肌MyHClmRNA在母猪显著大于公猪（P〈0．05）,而MyHC2amRNA在母猪显著小于公猪（P〈0．05）。背最长肌和半腱肌AdpR2mRNA的表达分别与MyHCl正相关（P〈0．05）,半腱肌AdpRlmRNA的表达与MyHC2x正相关（P〈0．05）。结果表明,皖南花猪骨骼肌组织中AdpR和MyHC的基因表达有特定的发育模式和组织特异性,且有一定性别差异。 相似文献
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T. E. Weber B. J. Kerr M. E. Spurlock 《Journal of animal physiology and animal nutrition》2008,92(5):569-577
Soy protein regulates adiponectin and peroxisome proliferator‐activated receptor α (PPARα) in some species, but the effect of dietary soy protein on adiponectin and PPARα in the pig has not been studied. Therefore, the objective of this study was to determine whether soya bean meal reduction or replacement influences serum adiponectin, adiponectin mRNA, serum metabolites and the expression of PPARα and other genes involved in lipid deposition. Thirty‐three pigs (11 pigs per treatment) were subjected to one of three dietary treatments: (i) reduced crude protein (CP) diet containing soya bean meal (RCP‐Soy), (ii) high CP diet containing soya bean meal (HCP‐Soy) or (iii) high CP diet with corn gluten meal replacing soya bean meal (HCP‐CGM) for 35 days. Dietary treatment had no effect on overall growth performance, feed intake or measures of body composition. There was no effect of dietary treatment on serum adiponectin or leptin. Dietary treatment did not affect the abundance of the mRNAs for adiponectin, PPARα, PPARγ2, lipoprotein lipase or fatty acid synthase in adipose tissue. The mRNA expression of PPARα, PPARγ2, lipoprotein lipase or fatty acid synthetase in loin muscle was not affected by dietary treatment. In liver tissue, the relative abundance of PPARα mRNA was greater (p < 0.05) in pigs fed the HCP‐Soy diets when compared to pigs fed RCP‐Soy or HCP‐CGM diets. Hepatic mRNA expression of acyl‐CoA oxidase or fatty acid synthase was not affected by dietary treatment. Western blot analysis indicated that hepatic PPARα protein levels were decreased (p < 0.05) in pigs fed the RCP‐Soy diets when compared to pigs fed the HCP‐Soy diets. These data suggest that increasing the soy protein content of swine diets increases hepatic expression of PPARα without associated changes in body composition. 相似文献
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冷应激对猪脂肪、肝脏、心脏与肾脏组织中PPARγ2 mRNA及蛋白表达的影响 总被引：1，自引：0，他引：1
为了研究冷应激对机体的影响及过氧化物酶体增殖物激活受体γ2（PPARγ2）在冷应激中的作用,本试验选用30头军牧一号猪,并随机分成5组,包括常温对照组和4个冷应激组。常温组在（21±2）℃饲养,冷应激组的温度设置分别为：（-10±2）、（-5±2）、（0±2）、（5±2）℃。冷应激2h屠宰后分别采集猪腹腔脂肪、颈部皮下脂肪、胸部皮下脂肪、肝脏、心脏与肾脏组织样品提取总RNA与总蛋白。通过荧光定量PCR技术检测了PPARγ2mR-NA的表达量;通过Western blot检测PPARγ2蛋白表达量。结果显示,各温度组猪腹腔脂肪、颈部皮下脂肪、胸部皮下脂肪、肝脏、心脏PPARγ2mRNA及蛋白表达量总体上差异显著（P〈0.01）,肾脏PPARγ2mRNA表达量总体显著升高,但其蛋白表达量总体呈下降趋势。结果表明,PPARγ2对冷刺激非常敏感,它除了可以调节冷应激时的脂肪代谢平衡,还对心脏有较好的保护作用,但是对肾脏的作用有限。本试验显示了冷应激中PPARγ2在脂肪代谢与能量代谢中作用,为研究冷应激对机体的影响及畜禽品质的提高奠定了基础。 相似文献
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【目的】探索二十二碳六烯酸(DHA)对高脂饲粮诱导肝脏脂肪积累的预防机制。【方法】将32只雄性SPF级C57BL/6小鼠均分为4组,对照组(Con)饲喂普通饲粮,模型组(Model)饲喂高脂饲粮,DHA组分别在高脂饲粮中添加0.2 g DHA(DHAL)和1.0 g DHA(DHAH),饲喂周期为20周。饲喂期间每天称量体重及食物重量,计算摄食量;饲喂结束,采集肝脏和血液,ELISA法检测肝脏脂联素和血清甘油三酯(TG)的含量,实时荧光定量PCR检测新生脂肪合成关键酶(SREBP-1c、FAS)、脂肪酸氧化关键基因(PPARα、PPARγ、CPT-1A和ACOX)、线粒体基因(PGC-1α)、褐色脂肪化基因(Prdm16、UCP1)的表达,Western blotting法检测肝脏磷酸化ACC、AMPK和AKT蛋白的表达。【结果】与Con组相比,Model组终体重、体脂重量和TG含量均显著增加(P<0.05),肝脏脂联素浓度显著降低(P<0.05)。与Model组相比,DHAL和DHAH组终体重、体脂重量和TG含量均显著降低(P<0.05),肝脏脂联素水平显著增加(P... 相似文献
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由断奶仔猪皮下脂肪分离血管基质细胞,增殖培养至80%融合时以10-6mol/L地塞米松处理48 h,同时设地塞米松拮抗剂RU486对照组。采用油红O染色提取法测定细胞内甘油三酯含量,相对定量实时荧光PCR方法检测细胞perilipin,GR,C/EBPβ,PPARγmRNA表达。结果显示,地塞米松显著增加细胞甘油三酯含量(P0.05),添加RU486后这种作用显著降低(P0.05);地塞米松处理组细胞中perilipin,PPARγ的mRNA表达显著高于对照组(P0.05),而C/EBPβmRNA表达较对照组显著降低(P0.05);GR mRNA表达与对照组相比无显著差异。以上结果提示:地塞米松能显著促进猪原代脂肪细胞的分化,这种作用可能通过上调perilipin mRNA表达而实现,其中PPARγ基因参与了调节。 相似文献
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Mahesh Gupta Krushna Bibichand Bahiram Vikas Muralidhar Sardar Jayant Pandurang Korde Swapnil P. Magar Sachin Wasudeorao Bonde Nitin Vasantrao Kurkure 《Reproduction in domestic animals》2019,54(9):1291-1303
Adiponectin is an adipocyte‐derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. The current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors AdipoR1 and AdipoR2 in ovarian follicles of different developmental stages in water buffalo (Bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qPCR, western blotting and immunohistochemistry were applied to demonstrate mRNA expression, protein expression and immunolocalization, respectively. The results indicate that adiponectin, AdipoR1 and AdipoR2 were present in granulosa cells (GC) and theca interna (TI) of ovarian follicles and the expression of adiponectin, AdipoR1, AdipoR2 in GC and AdipoR1 and AdipoR2 in TI increased with increase in follicle size (p < .05). Expression of adiponectin was high in small and medium size follicles in TI. The adiponectin and its receptors were immunolocalized in the cytoplasm of GC and TI cells. Further, in the in‐vitro study, GCs were cultured and treated with recombinant adiponectin each at 0, 1 and 10 µg/ml alone or with follicle stimulating hormone (FSH) at 30 ng/ml) or Insulin‐like growth factor I (IGF‐I) at 10 ng/ml for 48 hr after obtaining 75%–80%s confluency. Adiponectin at 10 µg/ml increased IGF‐I‐induced estradiol (E2) and progesterone (P4) secretion and FSH‐induced E2 secretion from GC and also increased the abundance of factors involved in E2 and P4 production (cytochrome P45019A1 [CYP19A1] and 3‐beta‐hydroxysteroid dehydrogenase [3β‐HSD]). In conclusion, this study provides novel evidence for the presence of adiponectin and its receptors in ovarian follicles and modulatory role of adiponectin on steroid production in buffalo. 相似文献
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A. De Rosa M.L. Monaco E. Nigro O. Scudiero M. D'Andrea F. Pilla G. Oriani A. Daniele 《Domestic animal endocrinology》2013
Adiponectin's beneficial effects are mediated by the AdipoR1 and AdipoR2 receptors (AdipoRs). The pig is a good model to study complex disorders such as obesity. We analyzed the expression of adiponectin, AdipoRs and some key molecules of energy metabolism (AMP-activated protein kinase α [AMPKα], p38 mitogen-activated protein kinase [p38 MAPK], and PPARα) in 2 pig breeds that displayed an opposite genetic behavior for energy metabolism: Casertana (CE), a fat-type animal, and Large White (LW), a lean-type animal. Muscle, liver, visceral and subcutaneous adipose tissues, and brain tissues were examined. The AdipoRs cDNA sequences were identical in the 2 breeds. AdipoRs mRNA expression, measured in all tissues, was significantly lower only in the 2 adipose tissues of CE pigs (P < 0.05). The muscle expression of AdipoRs, AMPKα, p38 MAPK, and PPARα was lower in CE than in LW animals (P < 0.01, P < 0.05, P < 0.01, P < 0.01, respectively). In liver, no molecule differed between breeds. The expression of both AdipoRs in visceral and subcutaneous adipose tissues was lower in CE pigs (P < 0.01). In brain, AdipoR1 and AMPKα expression was lower in CE pigs (P < 0.01), whereas AdipoR2 tended to be lower in CE than LW pigs (P = 0.05). In conclusion, our results suggest that tissue-specific downregulation of Adiponectin, AdipoRs, and of the key molecules of energy metabolism may be associated with the tendency of CE pigs to accumulate fat. 相似文献