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本文通过鸡胚接种的方法就蜂胶乙醇浸出物对NDV的灭活作用进行了试验观察，结果表明常温下3min，1mg/ml的47.5%乙醇烯释液对ND克隆－30具有灭活作用。含蜂胶乙醇出物0.1mg/ml的95％乙醇稀释液，含蜂胶乙醇出物10mg/ml的47．5％乙醇稀释,含峰胶乙醇浸出物10mg/ml的生理盐水稀释液，37℃3min，含蜂胶乙醇浸出物0.1mg/ml的95％乙醇稀释液，含蜂胶乙醇出物0.1mg/ml的47．5％乙醇稀释液，含蜂胶乙醇浸出物10mg/ml的生理盐水稀释液均对ND克隆－30具有完全灭活作用。 相似文献
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本文通过鸡胚接种的方法就蜂胶乙醇浸出物对NDV的灭活作用进行了试验观察,结果表明常温下3分钟,含蜂胶乙醇浸出物0.1mg/ml的95%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的47.5%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的生理盐水稀释液;37℃3分钟,含蜂胶乙醇浸出物0.1mg/ml的95%乙醇稀释液、含蜂胶乙醇浸出物0.1mg/ml的47.5%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的生理盐水稀释液均对ND克隆-30具有完全灭活作用;常温下3分钟,1mg/ml的47.5%乙醇稀释液对ND克隆-30具有灭活作用。 相似文献
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本文通过鸡胚接种的方法就蜂胶乙醇浸出物对禽流感H9亚型Shandong／chicken／98株的灭活作用进行了研究。结果表明，常温下3分钟、30分钟，37℃3分钟、30分钟，含蜂胶乙醇浸出物0．1mg/mL的95％乙醇稀释液，含蜂胶乙醇浸出物2．5mg／mL的47．5％乙醇稀释液。含蜂胶乙醇浸出物10mg/mL的生理盐水稀释液，接种鸡胚后病毒HA测定为0。这充分说明以上浓度蜂胶乙醇浸出物对禽流感H9亚型Shandong／chicken／98株具有完全的灭活作用。37℃3分钟、30分钟，含蜂胶乙醇浸出物0．1mg／mL的47．5％乙醇稀释液，含蜂胶乙醇浸出物6．25mg/mL的生理盐水稀释液对禽流感H9株有部分灭活作用。 相似文献
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蜂胶是一种具有广谱生物学活性的天然物质,本文就蜂胶对禽源多杀性巴氏杆菌与大肠杆菌的抑菌作用进行了试验观察,结果表明0．05％－5％（g/ml)的蜂胶乙醇浸出物对多杀性巴氏杆菌具有完全抑杀作用,0．1％－5％(g/ml)的蜂胶乙醇浸出物对大肠杆菌具有完全抑杀作用;0．01％（g/ml)的蜂胶乙醇浸出物对多杀性巴氏杆菌和大肠杆菌具有部分抑制作用。 相似文献
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禽霍乱蜂胶菌苗是以禽巴氏杆菌C_(48-1)(A:5)株加蜂胶乙醇浸液为佐剂制备的灭活菌苗。每ml菌苗含菌量为100亿,蜂胶干物质15mg。禽霍乱蜂胶菌苗对鸡具有良好的安全性与免疫原性,每只鸡接种1ml,7天即产生坚强免疫力,近期像护率(7～25天)为100％(35/35)。 相似文献
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应用冻精活力、解冻后4小时内生存指数、顶体完整率、谷草转氨酶(GOT)释放量等指标，在以前筛选稀释液、添加保护成分的基础上，对蔗糖组(0．8g／100ml，1．6g／100ml，2．4g／100m1)、谷氨酸组(20mg／100ml，30mg／100ml，40mg／100m1)及柠檬酸组(1．972g／100ml，2．185g／100ml，2．399g／100m1)三因素按正交设计L9(3^4)组合，比较冷冻波尔山羊精液的效果。稀释液优化的最佳组成为Tris4．361g，葡萄糖0．6540g，蔗糖1．6g，柠檬酸1．972g，谷氨酸0．04g，卵黄18ml，甘油6ml，青霉素、链霉素各10万u，双蒸水100ml。应用该配方制作的波尔山羊冻精进行配种，一个情期受胎率高达65．77％(146／222)，其中，在贵州乌当区种羊场一个情期不返情率66％(33／50)，产羔率84．85％(28／33，其中2只流产羔)，说明本试验筛选的最佳稀释液配方完全可以在生产中推广应用，并可获得较为理想的结果。 相似文献
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鸭传染性浆膜炎蜂胶疫苗研制 总被引：9，自引：0，他引：9
从重庆疫鸭中分离到2株细菌，经细菌形态学、生理生化指标鉴定，证明为鸭疫里氏杆菌（Riemerella anatipestifer,RA)。用改良的血清酵母肉汤进行培菌培养，将2菌株菌液混合（1：1，体积比），经甲醛灭活后加入蜂胶左剂，制成蜂胶灭活疫苗。用其免疫5－7日龄雏鸭，一免后7、10、15d皮下注射RA混合菌液攻毒（0．2mL/只）,平均保护率达66．67％；10d后二免，二免后7、10d攻毒（0．2mL／只）,平均保护率达94．47％。 相似文献
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Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5 总被引：1，自引：0，他引：1
Fischer G Cleff MB Dummer LA Paulino N Paulino AS de Oliveira Vilela C Campos FS Storch T D'Avila Vargas G de Oliveira Hübner S Vidor T 《Veterinary immunology and immunopathology》2007,116(1-2):79-84
Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines. 相似文献
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J Hwang 《American journal of veterinary research》1975,36(11):1683-1684
The comparative thermostability of 4 duck hepatitis (DH) viruses were tested at various temperatures for different times. Titer of duckling-passaged, pathogenic DH virus decreased from 10(4.50) to 10(2.33) and 10(2.20) median infective doses (ID50/0.1 ml, respectively, in 2 tests; titer of chicken embryo-passaged, nonpathogenic, but embryo-lethal, DH virus decreased from 10(6.00) to 10(0.46) and from 10(6.62) to 10(0.63) ID50/0.1 ml, respectively; duck embryo fibroblast culture-passaged and duck embryo liver cell culture-passaged, chicken ebryo-infective, but nonlethal, DH viruses were completely inactivated or nearly so after being kept at 56 C for 30 minutes. Duckling-passaged DH virus was not detected on day 21, whereas 10(0.62) ID50 of chicken embryo-passaged DH virus per 0.1 ml remained on day 32 when being kept at 37 C. Titer of chicken embryo-passaged DH virus decreased from 10(7.00) to 10(1.16) ID50/0.1 ml after being kept at room at room temperature for 150 days, to 10(5.17) ID50/0.1 ml after being kept at 4 C for 70 weeks, to 10(6.17) ID50/0.1 ml after being kept at -20 C for 70 weeks, and to 10(6.38) ID50/0.1 ml after being kept at -60 C for 1 year. 相似文献
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D T Shen T B Crawford J R Gorham T C McGuire 《American journal of veterinary research》1977,38(8):1217-1219
Twelve chemicals and commercial disinfectants were tested for inactivation of equine infectious anemia virus. In the presence of 10% bovine serum, all chemicals inactivated 4 log10 (based on 0.1 ml) of the virus within 5 minutes at 23 C. A reduction of at least 4 log10 was observed when the virus was exposed for 1 minute to substituted phenolic disinfectants (3 commercial preparations and sodium orthophenylphenate), halogen derivatives (iodophor and sodium hypochlorite), chlorhexidine, and 70% ethanol. Sodium hydroxide (5%), 2% formalin, and 2% glutaraldehyde were slower to inactivate the virus, but achieved 4 log10 reduction in titer by 5 minutes' contact time. The susceptibility of the equine infectious anemia virus to chemical disinfectants is similar to that of other enveloped viruses. 相似文献
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Bizimenyera SE Swan GE Chikoto H Eloff JN 《Journal of the South African Veterinary Association》2005,76(2):54-58
Peltophorum africanum (Fabaceae) is a deciduous tree widespread in southern Africa. The plant has many ethnomedical and ethnoveterinary uses. Root and bark decoctions are used to treat diarrhoea, dysentery, sore throat, wounds, back and joint pains, HIV-AIDS, venereal diseases and infertility. Pastoralists and rural farmers use the root and bark extracts to treat diarrhoea, dysentery, infertility, and to promote well-being and resistance to diseases in cattle. To evaluate these ethnobotanical leads, dried leaves, stem bark and root bark were extracted with ethanol, acetone, dichloromethane and hexane. Polyphenols in the extract were determined by the Folin-Ciocalteu method with gallic acid as standard. Qualitative antioxidant activity was screened by spraying thin layer chromatograms (TLC) of the extracts with 0.2% 1,1-diphenyl-2-picryl hydrazyl (DPPH), and quantified with Trolox equivalent antioxidant capacity (TEAC) assay. Minimum inhibitory concentration (MIC) and total antibacterial activity (TAA) were determined by serial microplate dilution for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis, with gentamicin as standard and tetrazolium violet as growth indicator. Acetone and ethanol extracted the largest quantity of material. Polyphenols concentration was 49.2% in acetone extract of the root and 3.8% in dichloromethane extract of the leaf. Antioxidant activity of at least 5 antioxidant compounds as measured by TEAC ranged from 1.34 (ethanol extract of the root) to 0.01 (hexane extract of the leaf). The total antibacterial activity (volume to which active compounds present in 1 g plant material can be diluted and still inhibit bacterial growth) was 1263 ml/g for ethanol extract of the root against S. aureus, and 800 ml/g for acetone extract of the root against P. aeruginosa. There was substantial activity against both Gram-positive and Gram-negative bacteria, with MIC values of 0.08 mg/ml for S. aureus and 0.16 mg/ml for P. aeruginosa. There is therefore a rationale for the traditional use of root and bark of P. africanum in treating bacterial infection related diseases. 相似文献
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【目的】制备鸭疫里默氏杆菌(Riemerella anatipestifer,RA)贵州流行株蜂胶灭活疫苗。【方法】选取RA血清2型贵州流行株(RA-SS-8株)为基础菌株,依次利用分光光度法和平板计数法测定细菌生长曲线,再进行灭活条件筛选,以蜂胶为佐剂制备RA贵州流行株蜂胶灭活疫苗,并进行无菌检验、安全性检验及免疫鸭攻毒保护性试验。【结果】分光光度法测定RA-SS-8株菌液D600 nm值随培养时间变化显示,细菌在0~3 h时增殖缓慢,在3~10 h时增殖趋势明显加快,在10 h以后增殖逐渐趋于平缓,随着培养时间的延长,细菌最终进入衰亡期;平板计数法结果显示,RA-SS-8株D600 nm值为0.1~0.8时,该菌处于对数生长期,且D600 nm值与活菌数呈现良好的线性关系;RA-SS-8株最佳灭活条件为0.2%甲醛溶液、37 ℃灭活12 h;蜂胶灭活疫苗含菌量为3.8×109 CFU/mL,蜂胶干物质含量为10 mg/mL;无菌检验巧克力琼脂培养基上未见菌落生长;安全性检验以2倍免疫剂量接种雏鸭在观察期内未表现出不良反应,大体病变观察未见明显病变;免疫鸭攻毒保护性试验显示,蜂胶灭活疫苗免疫组鸭对RA-SS-8株的攻击后保护率为70%,蜂胶灭活疫苗对试验鸭心脏、肝脏组织均具有良好的保护效果。【结论】本研究成功制备了RA贵州流行株蜂胶灭活疫苗,为蜂胶灭活疫苗制备和动物免疫试验奠定基础。 相似文献
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采用不同提取液、不同提取液倍数、不同提取方法对狼毒大戟进行了提取率的研究，同时对不同提取液及提取方法所得提取物质成分进行了分析。结果表明：以甲醇作为提取溶液采用6倍常温浸泡后索氏提取然后再常温浸泡的提取方法提取率最高，以95％乙醇作为提取液采用同样的提取方法其提取率次之；以95％乙醇、石油醚、乙酸乙酯作为提取液采用6倍常温浸泡后索氏提取再常温浸泡的提取方法较其他方法提取率高。甲醇在该方法下不仅提取率最高，而且其提取物所含成分也比较多，乙醇次之。由此可以得出，对于狼毒大戟的粗提取．采用甲醇或者乙醇作为提取溶液比较合适，其最佳提取方法为6倍常温浸泡后索氏提取然后再常温浸泡的提取方法。 相似文献
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A modified version of the test method of the Comité Européen de Normalisation (CEN) was developed using formic acid and three commercial disinfectants to evaluate virucidal activity against three non-enveloped viruses, bovine enterovirus type 1 (ECBO virus), mammalian orthoreovirus type 1 and bovine adenovirus type 1 (BAV 1). Determination of the effects of temperature was carried out at 20 and 10 degrees C. All tests with protein load used bovine serum albumin (BSA) and yeast extract. The investigations were performed in suspension tests and in carrier tests using poplar wood virus carriers. The carrier tests showed that ECBO virus could be inactivated at 20 degrees C with 1% formic acid within a 60 min reaction time. For disinfection of ECBO virus at 10 degrees C within 60 min, a 2% concentration of formic acid was necessary. Formic acid was ineffective against reovirus and bovine adenovirus and cannot be recommended as a reference disinfectant. Inactivation of ECBO virus and adenovirus type 1 using a disinfectant containing aldehydes and alcohols could be achieved, but only at room temperature. The disinfection of reovirus type 1 at room temperature with this product was possible without a protein load. This disinfectant exhibited disinfection ability at 10 degrees C at a concentration of more than 2% or with a longer exposure time. A disinfectant containing aldehydes was effective at room temperature but its effect was reduced in the presence of organic matter. Inactivation at 10 degrees C was found only against adenovirus. The fourth disinfectant, which contained peroxiacetic acid, inactivated all test viruses at a concentration of 0.5% within 15 min independent of temperature and protein load. 相似文献