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附红细胞体感染对猪红细胞免疫功能的影响 总被引：2，自引：0，他引：2
对急性附红细胞体病猪的红细胞免疫功能进行了检测。结果显示，感染附红细胞体病猪的RBC-CRl花环率显著下降，RBC-IC花环率变化不明显。表明附红细胞体侵袭红细胞的同时，破坏了红细胞膜表面的C3b受体，红细胞膜表面游离状态的C3b受体数量明显减少，导致机体红细胞的免疫黏附活性降低，红细胞免疫功能低下。 相似文献
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为探讨附红细胞体感染是否对仔猪的免疫水平产生影响,本试验从本地区某猪场选择4头附红细胞体自然感染发病仔猪和4头无附红细胞体感染的健康仔猪,进行了若干免疫学指标如红细胞C3b受体花环率、IC花环率;淋巴细胞转化率、T淋巴细胞Ea花环率;B淋巴细胞EAC花环率;外周血嗜中性白细胞吞噬率及吞噬指数的测定,并进行比较。试验结果表明,与健康仔猪相比,附红细胞体自然感染发病仔猪的红细胞C3b受体花环率和IC花环率明显降低;淋巴细胞转化率、T淋巴细胞Ea花环率明显降低;B淋巴细胞EAC花环率明显降低;外周血嗜中性白细胞吞噬率及吞噬指数明显降低,统计学分析表明,差异均极显著(P<0.01)。说明,附红细胞体感染对仔猪的红细胞免疫功能、细胞免疫水平、体液免疫水平及嗜中性白细胞功能均有明显的抑制作用。 相似文献
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本试验分别选用10头健康仔猪和附红细胞体感染仔猪,进行了血液红细胞压积、红细胞总数、血红蛋白、白细胞总数等生理指标的检测。结果显示,附红细胞体感染的仔猪红细胞压积(P0.01)、红细胞总数(P0.01)和血红蛋白(P0.01)显著低于健康仔猪,白细胞总数(P0.01)显著高于健康仔猪;附红细胞体感染的仔猪嗜中性白细胞(P0.05)、单核细胞(P0.05)显著高于健康仔猪,嗜酸性白细胞(P0.05)和淋巴细胞(P0.01)显著低于健康仔猪。结果表明,附红细胞体的侵袭引起猪血液指标的变化是猪附红细胞体病的共同特征。 相似文献
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猪附红细胞体实验室检查方法 总被引：4，自引：0，他引：4
附红细胞体病是广泛存在于人和动物体内的一种人畜共患病,是由附红细胞体寄生于人畜红细胞表面及其血浆、骨髓中而引起的,临床上以发热、贫血为主要特征。附红细胞体病具有高感染率、低发病率的特点,人工培养病原体没有获得成功,其形态结构和生物学特性均不完全清楚。现对附红细胞体实验室检查方法总结如下:1血液参数的变化经检测,感染附红细胞体的病猪红细胞总数可减少到1.78×109个/ml,血红蛋白降低到43g/L,均低于正常值。2鲜血压片法将等量鲜血和生理盐水混合,滴一滴于载玻片上加盖玻片,置油镜暗视野观察,可见到红细胞有形态学变化,在红… 相似文献
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附红细胞体自然感染对猪红细胞的影响 总被引：11，自引：1，他引：10
选择临床健康的 2月龄附红细胞体自然感染仔猪 1 5头 ,每周采血一次 ,连续 4次 ,观察红细胞的形态 ,并测定RBC、红细胞脆性 (最小脆性和最大脆性 )、红细胞ATPase和SOD活性、红细胞C3b受体 (E_C3bRR)率和红细胞免疫复合物花环 (E_ICR)率。检测结果 :随着猪附红细胞体感染强度的增强 ,所测定指标的变化无明显规律性或无明显差异 (P >0 .0 5 ) ,但红细胞脆性增高、红细胞ATPase和SOD活性及E_C3bRR率和E_ICR率均有降低趋势。表明猪感染附红细胞体后 ,红细胞易破裂而溶血、红细胞内外阳离子平衡失调、红细胞抗氧化能力降低、红细胞免疫功能下降。 相似文献
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附红细胞体病是由附红细胞体引起的一种人兽共患传染病,虽早在1928年就被发现,但由于多呈隐性感染,所以易被人们忽视,直至上世纪80年代,由于对畜牧业造成巨大危害,甚至危及人身健康,才逐渐被重视起来。到目前为止,国内大部分研究仅局限于该病的流行病学调查、诊断和治疗,但有关致病机理方面的研究还不多。附红细胞体的种类很多,现已正式命名的有14种,温氏附红体(E.wenyoni)是引起牛附红体病的病原体。本试验研究了不同感染程度牛红细胞免疫功能及血液中ANAE T淋巴细胞百分率的变化,旨在为探讨附红细胞体病的发病机理提供科学理论依据。1… 相似文献
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Characterization of feline T and B cells 总被引：1，自引：0，他引：1
T Kuramochi M Takeishi T Ishida K Kato M Ishida 《American journal of veterinary research》1987,48(2):183-185
Feline peripheral-blood lymphocyte populations (n = 22) were examined for the following markers: rosette formation with guinea pig erythrocytes (GPE-T cells), rosette formation with human RBC (HRBC-T cells), rosette formation with sheep RBC, mixed rosette formation with GPE-T cells and HRBC-T cells (total T cells), erythrocyte antibody-complement rosettes, and surface immunoglobulin. An average of 28% +/- 7% (range, 16% to 39%) of the feline lymphocytes formed rosettes with GPE-T cells, and 27% +/- 7% (range, 11% to 36%), with HRBC-T cells. An average of 57% +/- 9% (range, 33% to 75%) of the lymphocytes formed mixed rosettes. The erythrocyte antibody-complement rosette-forming cells and surface immunoglobulin-bearing cells were found in peripheral blood lymphocytes (10% +/- 6% and 24% +/- 8%, respectively). The murine monoclonal antibodies OKT 11 and HuLy-m1, specific for a framework determinant of human E-rosette receptor antigens, cross-reacted with feline cell membrane molecules recognizing a bimolecular complex (45,000 to 50,000 daltons) similar to that described in persons. We investigated the distribution of these E-rosette receptor-like antigens on feline lymphocytes. By complement-mediated lymphocytotoxicity, about 30% of the feline lymphocytes expressed the antigens. When lymphocytes were treated with HuLy-m1 antibody, spontaneous rosette formation with HRBC-T cells was significantly inhibited. 相似文献
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Osmotic fragility of erythrocytes was tested in weaned pigs experimentally infected with Eperythrozoon (E.) suis. Acute eperythrozoonosis of splenectomized pigs led to an increase of osmotic fragility. It is supposed that E. suis infection causes a structural change in erythrocyte membrane. Possible mechanisms of this cell membrane injury are discussed. 相似文献
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免疫调节剂对球虫感染鸡的免疫学指标的影响 总被引：6，自引：0，他引：6
为探讨免疫调节剂对鸡球虫病的免疫反应调节作用 ,设计并进行了免疫调节剂对球虫感染鸡的免疫学指标影响的研究。测定指标包括 :淋巴细胞玫瑰花环细胞形成率 ,淋巴细胞转化率 ,红细胞免疫复合物花环形成率 ,红细胞 C3b受体花环形成率 ,脾脏重 /体重相对重 ;血清中 Ig G,Ig M,Ig A的 EL ISA P/ N值。结果各项免疫学指标的差异显著。表明胸腺素、黄芪多糖和卡介苗等免疫调节剂可提高球虫感染鸡的淋巴细胞玫瑰花形成率、淋巴细胞转化率。 相似文献
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Sheep antibodies against a pig E-rosette-forming lymphoblastic T lymphoma raised by two intravenous injections of 10(10) cells showed little lymphocytotoxic activity which could be absorbed with red cells, alveolar macrophages or kidney or liver cell homogenates. Bone marrow absorption yielded subpopulation specific antibody which binds to E rosette-forming cells (E.RFC) using either complement-mediated cytotoxicity or indirect antiglobulin rosette formation. In 30 blood lymphocyte preparations from 20 pigs with a range of approximately 20-85% E rosettes the mean E% 43.5 +/- 2.7 agreed with the % antigen+ cells by cytotoxicity mean = 42.6 +/- 2.7 and in each individual sample these figures also agreed closely. In samples of blood lymphocytes enriched and depleted for E rosettes, results of %E+ also agreed closely with % antigen+ cells. This relationship also held for thymocytes and the specific antibodies could be completely absorbed with thymocytes. These data show that the antibody identified peripheral and thymic E.RFC. Bound to lymphocytes the antibody inhibited E rosette-formation with sheep red blood cells (SRBC) in saline (S) and dextran (DS) and with pig RBC in dextran and in Ficoll, but did not affect B cells shown by immunofluorescence, direct antiglobulin rosette formation or Fc rosette-formation, either in saline or dextran, (which include T gamma cells). E rosette inhibition was dependent on antibody concentration, showing single and double sigmoid curves for S and DS rosettes respectively, consistent with differing ease of inhibition of the strong and weak rosette formation. The same spectrum of inhibition of rosette formation by antibody binding followed subsequent incubation with C'6-deficient rabbit serum, but with C'-sufficient serum resulted in loss of cells which require dextran for Fc rosette-formation (T gamma). Thus the serum reveals E rosette-forming T cells and their subpopulations, perhaps by binding to the SRBC receptor. 相似文献
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C H Miller A R Carbonell R Peng M R MacKenzie M Shifrine 《American journal of veterinary research》1978,39(7):1191-1194
Reference values for T and B lymphocytes were determined on lymphocytes from canine thymus, spleen, lymph node, bone marrow, and peripheral blood by use of erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosette assays, plus a direct fluorescent technique for assay of surface immunoglobulins. Numbers of T lymphocytes, indicated by E rosette formation with human erythrocytes, ranged from a low of 1% in the thymus to 13% in the peripheral blood, whereas B-lymphocyte numbers ranged from 3% (thymus) to 41% (bone marrow) and from 6% (thymus) to 36% (bone marrow), as indicated by EAC rosette formation or presence of surface immunoglobulins respectively. Stimulation of peripheral blood lymphocytes with either phytohemagglutinin or concanavalin A increased the total number of E-rosetting cells two to threefold, whereas the number of EAC-rosetting cells decreased by half. Further, the percentage of cells bearing Fc receptors increased after phytohemagglutinin stimulation. These results indicate the E rosette technique can be used to identify and to monitor a population of canine T lymphocytes. 相似文献
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选用24只3月龄、体质量（13±1.0）kg的雄性波尔山羊,随机分为4组,每组6只,试验期60d。Ⅰ组为对照组（饲料添加微量元素）;Ⅱ组（低剂量组）、Ⅲ组（中剂量组）、Ⅳ组（高剂量组）分别肌肉注射＂生命元＂0.5,1.0,2.0mL/kg。试验前1d及试验后15,30,45,60d静脉采血测定外周血液T淋巴细胞酸性α-醋酸萘酯酶阳性率（ANAE）、红细胞补体受体花环率（C3bRR）及免疫复合花环率（ICR）;试验后30,45,60d测定外周血液淋巴细胞周期指标。结果表明：（1）＂生命元＂能提高波尔山羊血液中T淋巴细胞酸性α-醋酸萘酯酶阳性率,且在试验后45,60d试验组显著高于对照组（P〈0.05或P〈0.01）,并以中剂量组效果最佳。（2）＂生命元＂能够提高波尔山羊红细胞补体受体花环率,降低红细胞的免疫复合物花环率,增强波尔山羊的红细胞免疫功能。（3）与对照组相比,试验组不同程度降低G0/G1期和DNA的百分含量,提高S、G2-M期DNA的百分含量以及细胞分裂指数（PI）和DNA百分含量,并在一定时期呈现显著性（P〈0.05或P〈0.01）差异,并以中剂量组效果最佳。 相似文献
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Erythrocyte membrane alterations associated with the attachment and replication of Eperythrozoon suis: a light and electron microscopic study 总被引：8，自引：0，他引：8
Erythrocytes from pigs with experimental porcine eperythrozoonosis were examined using light microscopy and scanning and transmission electron microscopy. Three distinct forms of Eperythrozoon suis were attached to the plasma surface of erythrocyte membrane. Erythrocytes were initially parasitized by one or several immature forms. Immature forms enlarged and developed into juvenile and mature forms. The parasite replicated by budding of small immature forms from larger immature forms, juvenile forms, and mature forms. Small immature forms attached to adjacent membrane of the same erythrocyte or a nonparasitized erythrocyte. E. suis organisms were intimately associated with, but distinctly separated from erythrocyte membrane by a 30-nm electron lucent zone. Cell membrane in this area was denser than adjacent nonparasitized membrane. Early interaction between erythrocyte membrane and small immature forms resulted in no membrane deformation, but as this form enlarged, it later became embedded in a deep cup-like membrane invagination. As the immature form developed into the juvenile form a shallow, broad-based depression was observed in the membrane. A similar depression that covered a greater surface area was observed in the membrane parasitized by the mature form. The interaction between the parasitic forms and erythrocyte membrane resulted ultimately in severe membrane deformation. Parasite-membrane interactions may play an important role in the development of misdirected immune responses in experimental porcine eperythrozoonosis. 相似文献
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为了研究早期断奶应激对仔猪红细胞免疫功能的影响,试验采用同窝杜×(大×长)三元杂交健康仔猪12只,分别于断奶前3天、断奶后3天及断奶后2周进行红细胞受体C3b花环试验、免疫复合物IC花环试验及血常规检验,观察断奶应激对仔猪红细胞免疫功能、红细胞数量及血红蛋白含量影响的动态变化。结果表明:断奶应激导致仔猪红细胞C3b受体花环率(C3bRR率)、红细胞总数和血红蛋白含量降低,IC花环率(ICR率)的变化明显。说明断奶应激可导致仔猪红细胞免疫功能下降,红细胞免疫功能低的仔猪更容易发生(断奶)应激性腹泻。 相似文献